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    Differential Leukocyte Count

    Uploaded by

    Mariam Khalil
    This document provides instructions for performing a manual differential leukocyte count. Key steps include: 1. Making a well-stained thin blood film on a clean slide using fresh blood without anticoagulant or EDTA anticoagulated venous blood. 2. Examining the smear under the microscope using different magnifications and counting at least 100 leukocytes using a classification method. 3. Reporting the results as percentages of different types of leukocytes and calculating their absolute counts using a provided formula involving the total white blood cell count. 4. Reference values for the relative and absolute counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils are also listed

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    Differential Leukocyte Count

    Uploaded by

    Mariam Khalil
    144 views2 pages
    This document provides instructions for performing a manual differential leukocyte count. Key steps include: 1. Making a well-stained thin blood film on a clean slide using fresh blood without anticoagulant or EDTA anticoagulated venous blood. 2. Examining the smear under the microscope using different magnifications and counting at least 100 leukocytes using a classification method. 3. Reporting the results as percentages of different types of leukocytes and calculating their absolute counts using a provided formula involving the total white blood cell count. 4. Reference values for the relative and absolute counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils are also listed

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    WBCs

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    Differential leukocyte count

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    This document provides instructions for performing a manual differential leukocyte count. Key steps include: 1. Making a well-stained thin blood film on a clean slide using fresh blood without anticoagulant or EDTA anticoagulated venous blood. 2. Examining the smear under the microscope using different magnifications and counting at least 100 leukocytes using a classification method. 3. Reporting the results as percentages of different types of leukocytes and calculating their absolute counts using a provided formula involving the total white blood cell count. 4. Reference values for the relative and absolute counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils are also listed

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    144 views2 pages

    Differential Leukocyte Count

    Uploaded by

    Mariam Khalil
    This document provides instructions for performing a manual differential leukocyte count. Key steps include: 1. Making a well-stained thin blood film on a clean slide using fresh blood without anticoagulant or EDTA anticoagulated venous blood. 2. Examining the smear under the microscope using different magnifications and counting at least 100 leukocytes using a classification method. 3. Reporting the results as percentages of different types of leukocytes and calculating their absolute counts using a provided formula involving the total white blood cell count. 4. Reference values for the relative and absolute counts of neutrophils, lymphocytes, monocytes, eosinophils, and basophils are also listed

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 2

    Manual differential leukocyte count

    Introduction:
    A differential white cell count is the assigning of leucocytes to their individual categories, this
    categorization being expressed as a percentage or, when the WBC is available, as an absolute
    count..

    Materials:
    1- Clean, dust- free, grease-free glass slide (better to use glass slide with frosted end for
    labeling).
    2- Spreader (smooth end).
    3- Fresh blood without anticoagulant or EDTA anticoagulated venous blood. (Do not use
    heparinized venous blood because it leads to blue back ground).
    4- Leishman’s stain

    Methodology:
    1- Make well-made well stained thin blood film.
    2- Examine the smear systematically by using X10,X40 and X100.
    3- Start counting in an ideal area (not more than 50% of RBCs are overlapped).
    4- Classify at least 100 leukocytes either by battlement or longitudinal method.
    5- Report results of the 100 cells classified as percentage.
    6- Calculate the absolute leukocyte count by using the following formula:

    Absolute leukocyte count= TWBCs count X relative count

    100

    1
    Reference values:

    Absolute count Relative count

    Neutrophils 2.0–7.0 × 109/l (40–75%)

    Lymphocytes 1.0–3.0 × 109/l (20–45%)

    Monocytes 0.2–1.0 × 109/l (2–10%)

    Eosinophils 0.02–0.5 × 109/l (2–6%)

    Basophils 0.02–0.1 × 109/l (<1–2%)

    *WBC Correction for the Presence of Nucleated Red Blood Cells (nRBCs)

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