221-79041C AOC20i 20s IM EN

221-79041C
Dec. 2020
AUTOINJECTOR
AOC™-20i/AOC™-20i Plus
AUTOSAMPLER
AOC™-20s
Instruction Manual
Read this manual thoroughly before you use the product.
Keep this manual for future reference.
This page is intentionally left blank.
Introduction
Read this Instruction Manual thoroughly before using the product.
Thank you for purchasing this product.

This manual describes the installation, operation, usage cautions, and accessories and options for this product.
Read this manual thoroughly before using the product and operate the product in accordance with the instructions
in this manual.
Keep this manual for future reference.
IMPORTANT
•If the user or usage location changes, ensure that this Instruction Manual is always kept together with the product.
•If this manual or a product warning label is lost or damaged, immediately contact your Shimadzu representative to
request a replacement.
•To ensure safe operation, read all Safety Instructions before using the product.
•To ensure safe operation, contact your Shimadzu representative if product installation, adjustment, re-installation
(after the product is moved), or repair is required.
Notice •Information in this manual is subject to change without notice and does not represent
a commitment on the part of the vendor.
•Any errors or omissions which may have occurred in this manual despite the utmost
care taken in its production will be corrected as soon as possible, although not
necessarily immediately after detection.
•All rights are reserved, including those to reproduce this manual or parts thereof in
any form without permission from Shimadzu Corporation.
•AOC, GCsolution, Chromatopac, and GCMSsolution are trademarks of Shimadzu
Corporation.
Windows is a registered trademark of Microsoft Corporation in the United States and/
or other countries.
Third-party trademarks and trade names may be used in this publication to refer to
either the entities or their products/services, whether or not they are used with
trademark symbol "TM" or "®".
•In this booklet, Autoinjector AOC-20i/AOC-20i Plus is indicated as "AOC-20i" or
"Autoinjector".
2012-2020 Shimadzu Corporation. All rights reserved.

Original version is approved in English.
AOC-20i/AOC-20i Plus/AOC-20s i
Indications Used in This Manual
Precaution symbols are indicated using the following conventions:

Indication Meaning
Indicates a potentially hazardous situation which, if not avoided, could result in serious injury or possibly death.
Warning
Indicates a potentially hazardous situation which, if not avoided, may result in minor to moderate injury or
Caution
equipment damage.
Emphasizes additional information that is provided to ensure the proper use of this product.
Note
Safety Instructions
The AOC-20i Autoinjector and AOC-20s Autosampler automatically inject liquid samples into a gas chromatograph.
To ensure safe operation of the instrument, read these Safety Instructions carefully before use. Observe all of the
WARNINGS and CAUTIONS described in this section. They are extremely important for safety.
1. Do not use for any purpose other than the above-mentioned types of analyses.
2. Follow the procedures described in the Instruction Manual, otherwise the instrument may not be used safely.
3. Observe all warnings and precautions.
4. Do not disassemble or modify the unit without the express approval of an authorized Shimadzu representative.
5. For internal repair of the product, contact your Shimadzu representative.
 Product Applications
! WARNING
• Do NOT use the product for purposes other than those specified.
This product is automatically inject liquid samples into a gas chromatograph.
Using it for an unspecified purpose may result in an accident.
This product (including accessories) is manufactured for measuring and industrial purposes, and not suitable for
household usage.
• Safety regulations and standards

For notifications on installation and safety controls, follow the necessary procedures in compliance with the laws
and regulations applicable in the country where the product is used.
 Installation
To ensure safe operation, contact your Shimadzu representative for product installation, adjustment, or re-
installation (after the product is moved) is required.
ii AOC-20i/AOC-20i Plus/AOC-20s
 Operation
! WARNING
• USE CARE WHEN HANDLING FLAMMABLE SOLVENTS OR SAMPLES
To prevent flammable solvents or samples (such as carbon disulfide) from evaporating and filling the installation
site, securely tighten the cap that holds the septum in place on all vials (including rinse solvent and waste liquid
vials).
@ NOTE
Installation Precautions
Be sure to use the optional sample cooling fan when working with solvents with particularly strong flammability, such
as carbon disulfide.
For other flammable solvents or samples, it is also recommended to use the sample cooling fan.
• Prevent spills by never exceeding the waste liquid vial capacity.

If the waste liquid vial overflows or the vapor fills the installation site, the heat from the injection port could ignite
the liquid or vapor, causing a fire.
 Inspection and Maintenance
! WARNING
• Contact your Shimadzu Service Representative for removing the cover of the power supply unit.
Otherwise, electric shock could occur.
AOC-20i/AOC-20i Plus/AOC-20s iii

 In an Emergency
If any abnormality is found in Autoinjector AOC-20i/Autosampler AOC-20s, bring the device to an emergency
shutdown.
After the emergency shutdown, DO NOT restart immediately, but inspect the autoinjector/autosampler carefully
and contact your Shimadzu representative as necessary.
Emergency Shutdown Operation
1. Turn OFF the switch on the power supply unit.

2. Unplug the power cable to shut off the power supply.
For the autoinjector/autosampler featuring a built-in power supply unit in the gas chromatograph, bring the GC
to an emergency shutdown by the following procedure.
1. Turn OFF the gas chromatograph power switch.
2. Shut off the power supply to the gas chromatograph.
•If the power cable is fixed with screws to the switchboard, turn OFF the switch on the switchboard.
•If the power cable is connected with a plug, unplug the power cable.
Power switch
Power cable
Figure 1 Power switch and power cable (External power supply unit)
 During a Power Outage

Take the following measures in the event of power outage.
1. Switch OFF power to the autoinjector and the autosampler (the gas chromatograph for a built-in power supply
unit).
2. After the power outage recovery, use the standard startup procedure to start the autoinjector and the
autosampler.
iv AOC-20i/AOC-20i Plus/AOC-20s
Warning Labels
In order to ensure safety, warning labels are attached in places requiring caution.
If a warning label is lost or damaged, obtain a new label through your Shimadzu representative and attach it in the
correct position. See "Warning Labels on the Equipment" in this Instruction Manual for details on the positions where
the labels are attached.
 Autoinjector
Figure 2
 Power Supply Unit
HIGH
VOLTAGE
Figure 3
AOC-20i/AOC-20i Plus/AOC-20s v
Warranty
Shimadzu provides the following warranty for this product.
1. Period: Please contact your Shimadzu representative for information about the period of this
warranty.
2. Description: If a product/part failure occurs for reasons attributable to Shimadzu during the warranty
period, Shimadzu will repair or replace the product/part free of charge. However, in the
case of products which are usually available on the market only for a short time, such as
personal computers and their peripherals/parts, Shimadzu may not be able to provide
identical replacement products.
3. Limitation of Liability: 1) In no event will Shimadzu be liable for any lost revenue, profit or data, or for special,
indirect, consequential, incidental or punitive damages, however caused regardless of the
theory of liability, arising out of or related to the use of or inability to use the product, even
if Shimadzu has been advised of the possibility of such damage.
2) In no event will Shimadzu's liability to you, whether in contract, tort (including negligence),
or otherwise, exceed the amount you paid for the product.
4. Exceptions: Failures caused by the following are excluded from the warranty, even if they occur
during the warranty period.
1) Improper product handling
2) Repairs or modifications performed by parties other than Shimadzu or Shimadzu
designated companies
3) Product use in combination with hardware or software other than that designated by
Shimadzu
4) Computer viruses leading to device failures and damage to data and software, including
the product's basic software
5) Power failures, including power outages and sudden voltage drops, leading to device
failures and damage to data and software, including the product's basic software
6) Turning OFF the product without following the proper shutdown procedure leading to
device failures and damage to data and software, including the product's basic software
7) Reasons unrelated to the product itself
8) Product use in harsh environments, such as those subject to high temperatures or
humidity levels, corrosive gases, or strong vibrations
9) Fires, earthquakes, or any other act of nature, contamination by radioactive or hazardous
substances, or any other force majeure event, including wars, riots, and crimes
10) Product movement or transportation after installation
11)Consumables and equivalent items
Recording media such as CD-ROMs are considered consumable items.
* If there is a document such as a warranty provided with the product, or there is a separate contract agreed upon that includes warranty
conditions, the provisions of those documents shall apply.
vi AOC-20i/AOC-20i Plus/AOC-20s
After-Sales Service and Availability of Replacement Parts
After-Sales Service If any problem occurs with this product, perform an inspection and take
appropriate corrective action as described in "6 Troubleshooting" P.151 of this
manual.
If the problem persists, or the symptoms are not covered in the
Troubleshooting section, contact your Shimadzu representative.
Replacement Parts Availability Replacement parts for this product will be available for a period of seven (7)
years after the product is discontinued. Thereafter, such parts may cease to be
available.
Note, however, that the availability of parts not manufactured by Shimadzu
shall be determined by the relevant manufacturers.
Maintenance, Inspections, and Adjustment
In order to maintain the instrument's performance and obtain accurate measurement data, daily inspection and
periodic inspection/calibration are necessary.
•For daily maintenance, inspection, and replacement parts, see "5 Maintenance" of this Instruction Manual.
•Periodic inspection/calibration should be requested to your Shimadzu representative.
•Replacement cycles described for periodic replacement parts are rough estimate.
Replacement may be required earlier than the described replacement cycles depending on usage environment and
frequency.
Disposal Precautions
This product must be disposed of in compliance with the respective national or local government regulations.
AOC-20i/AOC-20i Plus/AOC-20s vii

Action for Environment (WEEE)
Equipment marked with this symbol indicates that it was sold on or after 13th
August 2005, which means it should not be disposed of with general
household waste. Note that our equipment is for industrial/professional use
only.
Contact Shimadzu service representative when the equipment has reached

the end of its life. They will advise you regarding the equipment take-back.
With your co-operation we are aiming to reduce contamination from waste electronic and electrical equipment and
preserve natural resources through re-use and recycling.
Do not hesitate to ask Shimadzu service representative, if you require further information.
viii AOC-20i/AOC-20i Plus/AOC-20s

Electromagnetic Compatibility
Descriptions of this section are only applied to the following models:

•S221-72315-38/48/58 AOC-20i for GC-2010
•S221-72315-38/48/58 AOC-20i for GC-2010Plus
•S221-72314-38/48/58 AOC-20i for GC-2014
•S221-73957-38/48/58 AOC-20i for GC-2025
•S221-80970-58 AOC-20i Plus for GC-2030
•S221-86003-58 AOC-20i Plus
•S221-72353-38, S221-72354-38/48/58 AOC-20i with external power supply unit
•S221-72300-31/41/58 AOC-20s
•S221-80975-58 AOC-20s for GC-2030
•S221-86001-58 AOC-20sU
This product complies with European standard EN61326-1, class A for electromagnetic interference (Emissions) and
basic electromagnetic environment requirement (Table1) for electromagnetic susceptibility (Immunity).
 EN61326-1 Emissions (Electromagnetic Interference)

This is a class A product and is not designed for use in a residential environment.
When this product causes an electromagnetic disturbance to devices being used near this product, create an
appropriate distance between those devices and this product in order to eliminate the disturbance.
 EN61326-1 Immunity (Electromagnetic Susceptibility)

Compliance with this standard does not ensure that the product can operate at a level of electromagnetic
interference that is stronger than the level tested. Interference stronger than the values specified in the standard
may cause the product to malfunction.
When installing or using this product, especially in an industrial location:

Locate the product away from any device emitting strong levels of electromagnetic noise.
Use a power source that is separated from the power source of any device emitting strong levels of
electromagnetic noise.
To prevent static electricity:

Prior to touching the product, the operator should be sure to discharge the static electricity stored in their body by
first touching a grounded metallic structure.
Do not touch any terminals or connectors that are not connected to cables while the product is turned ON.
AOC-20i/AOC-20i Plus/AOC-20s ix
x AOC-20i/AOC-20i Plus/AOC-20s
Contents
Introduction.............................................................................................................................. i
Indications Used in This Manual............................................................................................. ii
Safety Instructions .................................................................................................................. ii

Product Applications.......................................................................................................................... ii
Installation ......................................................................................................................................... ii
Operation...........................................................................................................................................iii
Inspection and Maintenance ............................................................................................................iii
In an Emergency .............................................................................................................................. iv
During a Power Outage.................................................................................................................... iv
Warning Labels....................................................................................................................... v
Autoinjector ....................................................................................................................................... v
Power Supply Unit............................................................................................................................. v
Warranty ................................................................................................................................ vi
After-Sales Service and Availability of Replacement Parts .................................................. vii
Maintenance, Inspections, and Adjustment.......................................................................... vii
Disposal Precautions............................................................................................................ vii
Action for Environment (WEEE) ...........................................................................................viii
Electromagnetic Compatibility ............................................................................................... ix

EN61326-1 Emissions (Electromagnetic Interference) .................................................................... ix
EN61326-1 Immunity (Electromagnetic Susceptibility) .................................................................... ix
1 Overview
1.1 Introduction ..........................................................................................................1
1.2 Components ........................................................................................................2
1.3 System Configuration and Operation...................................................................3
1.4 Unpacking............................................................................................................5
1.5 Accessories List...................................................................................................6
1.5.1 Autoinjector Standard Accessories .................................................................... 6
1.5.2 AOC Power Supply Unit ..................................................................................... 6
1.5.3 Autoinjector Installation Parts............................................................................. 8
1.5.4 Autosampler Standard Accessories ................................................................. 11
1.5.5 Autosampler Installation Parts (for GC-2014, GC-14A/B) ................................ 11
AOC-20i/AOC-20i Plus/AOC-20s xi
Contents
1.6 Features ............................................................................................................ 12

1.6.1 Autoinjector Features ....................................................................................... 12
1.6.2 Autosampler Features ...................................................................................... 12
1.6.3 Features Common to Autoinjector and Autosampler ....................................... 12
1.7 Specifications .................................................................................................... 13

1.7.1 Autoinjector Specifications ............................................................................... 13
1.7.2 Autosampler Specifications.............................................................................. 14
1.8 Part Names........................................................................................................ 15

1.8.1 Autoinjector ...................................................................................................... 15
1.8.2 Autosampler ..................................................................................................... 16
1.8.3 Power Supply Unit............................................................................................ 17
1.9 Operation Environment...................................................................................... 19
2 Autoinjector/Autosampler Installation
2.1 Installing the Autoinjector to the Gas Chromatograph....................................... 21
2.1.1 Installation to the Gas Chromatograph ............................................................ 21
2.1.2 Mounting Spacers on GC-2014/GC-14A/B ...................................................... 23
2.2 Mounting and Removing the Syringe ................................................................ 26

2.2.1 Mounting the Microsyringe ............................................................................... 26
2.2.2 Removing the Microsyringe.............................................................................. 35
2.3 Setting Vial Racks to the Rack Guide, Loading Rack into the Autoinjector....... 38
2.3.1 Setting Vial Racks to the Short Rack Guide..................................................... 38
2.3.2 Setting Vial Racks to the Long Rack Guide (Optional) .................................... 39
2.3.3 Loading Rack into the Autoinjector .................................................................. 41
2.3.4 Washing with 3 Different Solvents ................................................................... 45
2.4 Vial Types and Vial Mounting Procedure on Rack Guide.................................. 47

2.4.1 Vial Types ........................................................................................................ 47
2.4.2 Septum Replacement and Sample Volume in Vials......................................... 48
2.4.3 Vial Placement ................................................................................................. 55
2.5 Replacing INJ Port Septum ............................................................................... 57
2.6 Available Options............................................................................................... 62

2.6.1 Sample Cooling Fan (P/N S221-44995-91) ..................................................... 62
2.6.2 Long Rack (P/N S221-45622-91) ..................................................................... 62
2.6.3 FPD Cooling Fan (P/N S221-44996-91) .......................................................... 62
2.7 Installing the Autosampler (AOC-20s) [Optional]............................................... 64

2.7.1 Installing the Vial Racks ................................................................................... 64
2.7.2 Setting up Autoinjector Vial Racks ................................................................... 67
2.7.3 Using 4 ml Vials (Option) ................................................................................. 70
2.7.4 Precautions for Using the Autosampler............................................................ 70
xii AOC-20i/AOC-20i Plus/AOC-20s

Contents
2.7.5 Adjusting Sample Gripping Position (Teaching)............................................... 70

2.7.6 Mounting bracket for GC14/2014 (for AOC-20) (Optional)............................... 73
2.8 Controlling via Workstation ................................................................................75

2.8.1 Controlling AOC-20i,s via GCsolution/GCMSsolution
(on GC-2010/2010Plus/2014/2025, GCMS-QP2010) ...................................... 75
2.8.2 Controlling AOC-20i,s Using CBM-101/102
(on GCs Other than GC-2010/2010Plus/2014/2025) ....................................... 75
2.8.3 Installation for GCMS-QP5000......................................................................... 76
2.8.4 Chromatopac C-R7A Series GC Network Control............................................ 77
3 Operation
3.1 Basic Operation .................................................................................................79
3.1.1 Loading Vials in the Rack................................................................................. 79
3.1.2 Turning the Power ON/OFF ............................................................................. 80
3.1.3 AOC-20i Keypad .............................................................................................. 81
3.1.4 Setting Parameters .......................................................................................... 83
3.1.5 Basic Injection Procedure ................................................................................ 85
3.1.6 Operation Precautions ..................................................................................... 88
3.2 Display and Keypad Functions ..........................................................................89

3.2.1 Display and Arrow Keys ................................................................................... 89
3.2.2 Parameter Keys ............................................................................................... 89
3.2.3 MONITOR Key ................................................................................................. 90
3.2.4 ENTER Key ...................................................................................................... 91
3.2.5 RESET Key ...................................................................................................... 91
3.2.6 START Key ...................................................................................................... 91
3.2.7 STOP Key ........................................................................................................ 91
3.2.8 FUNCTION Key................................................................................................ 92
3.2.9 GC-2010/2010Plus/2014/2025 Menus and Autoinjector Parameters ............ 101
3.3 Sequential Analyses and Injection Modes .......................................................104

3.3.1 Sequential Analyses....................................................................................... 105
3.3.1.1 Preparation........................................................................................................... 105
3.3.1.2 Operation.............................................................................................................. 110
3.3.2 Priority Analysis.............................................................................................. 111
3.3.3 Injection Modes .............................................................................................. 113
3.4 Autosampler Operation....................................................................................117

3.4.1 Preparation..................................................................................................... 117
3.4.2 Power ON....................................................................................................... 122
3.4.3 Power Light .................................................................................................... 122
3.4.4 AOC Parameters ............................................................................................ 123
3.4.5 Priority Analysis.............................................................................................. 123
3.4.6 Solvent Flush Injection Mode ......................................................................... 124
3.5 Analysis Tips and Precautions.........................................................................126
AOC-20i/AOC-20i Plus/AOC-20s xiii

Contents
3.6 GCsolution....................................................................................................... 128

3.6.1 System Configuration..................................................................................... 128
3.6.2 Setting Parameters ........................................................................................ 129
3.7 CLASS-GC10 .................................................................................................. 130

3.7.1 System Configuration..................................................................................... 130
3.7.2 Setting Parameters ........................................................................................ 131
3.8 CLASS-VP....................................................................................................... 132

3.8.1 Autoinjector/Autosampler Configuration......................................................... 132
3.8.2 Pretreatment Setup Screen............................................................................ 132
4 External Control
4.1 Preparation ...................................................................................................... 134
4.2 External Control............................................................................................... 135

4.2.1 Transmission by Simple Procedure ............................................................... 135
4.2.1.1 Parameter Transmission (Setting) ........................................................................ 135
4.2.1.2 Parameter Reception and Status Monitoring........................................................ 136
4.2.1.3 Controlling Injector Operation ............................................................................... 137
4.2.1.4 Transmission Precautions .................................................................................... 137
4.2.2 Level 2 Transmission ..................................................................................... 138
4.2.2.1 Opening the Port .................................................................................................. 138
4.2.2.2 Transmission of Parameters (Setting) .................................................................. 138
4.2.2.3 Reception of Parameters and Status Monitoring .................................................. 138
4.2.2.4 Controlling the Autoinjector .................................................................................. 139
4.2.2.5 Closing the Port .................................................................................................... 139
4.2.3 Command List ................................................................................................ 140
4.3 Example Programs .......................................................................................... 142

4.3.1 Example Program for Simple Procedure Transmission ................................. 142
4.3.2 Example Program for Level 2 Transmission .................................................. 144
5 Maintenance
5.1 Cleaning the Vials............................................................................................ 145
5.2 Syringe Inspection and Maintenance .............................................................. 146
5.3 Consumable Parts and Standard Accessories ................................................ 147

5.3.1 Autoinjector Consumable Parts...................................................................... 147
5.3.2 Autoinjector/Autosampler Accessories........................................................... 147
5.4 Maintenance Parts........................................................................................... 148
5.5 Optional Parts.................................................................................................. 149
xiv AOC-20i/AOC-20i Plus/AOC-20s

Contents
6 Troubleshooting
6.1 Error Codes .....................................................................................................151
6.1.1 Autoinjector Error Codes ................................................................................ 151
6.1.2 Autosampler Error Codes............................................................................... 153
6.1.3 Sub-injector Error Codes................................................................................ 154
6.1.4 Miscellaneous Error Messages ...................................................................... 154
7 To Achieve Good Analysis Reproducibility

7.1 Precautions on Use of the Microsyringe ..........................................................155
7.2 Maintenance of the Microsyringe.....................................................................156
7.3 Precautions on Packing with Silica Wool.........................................................158
7.4 Precautions on Replacing the Septum ............................................................160
7.5 Other Precautions on Use ...............................................................................161
Index ............................................................................................. 163
AOC-20i/AOC-20i Plus/AOC-20s xv
Contents
xvi AOC-20i/AOC-20i Plus/AOC-20s

1 1 Overview
1.1 Introduction 1
Shimadzu Autoinjector AOC-20i/Autosampler AOC-20s automatically injects liquid samples to gas
chromatographs (GC). It can be mounted to Shimadzu gas chromatographs such as GC-14A/B, GC-15A,
GC-16A, GC-17A Ver.1/2/3, GC-1700, GC-18A, and GC-2010/2010Plus/2014/2025. 1
AOC-20i/AOC-20s for GC-2010/2010Plus/2014/2025 is a built-in power type, where the AOC power
supply unit is incorporated in the GC main unit.
The existing "External power type" can also be used with GC-2010/2010Plus/2014/2025, if the AOC-20i
1
installation kit is attached. See "5.5 Optional Parts" P.149.
1
1
1
1
1
1
1
1
1
1
1
1
1
AOC-20i/AOC-20i Plus/AOC-20s 1
1 Overview
1.2 Components
Autoinjector AOC-20i/Autosampler AOC-20s consists of three units: 1 Autoinjector, 2 Power supply unit,
and 3 Autosampler.
(The configuration without the autosampler is also available.)
1
1
3 3
Built-in
2 2
Built-in Power Type External Power Type
Figure 1-1
Name Part No. Remarks

1 Autoinjector S221-72310-41
2 Power supply Built-in For GC-2014 S221-72350-41 The terminals for the built-in power
unit power type supply unit are located on the gas
For GC-2010/2010Plus S221-72352-41 chromatograph back panel. (See
"1.8.3 Power Supply Unit" P.17)
For GC-2025 S221-73959-91
External power type S221-72347-42

3 Autosampler S221-72300-41
2 AOC-20i/AOC-20i Plus/AOC-20s
1.3 System Configuration and Operation
1.3 System Configuration and Operation

There are four ways to operate autoinjector/autosampler according to the GC configurations described
below [(1) to (4)], including the stand alone operation using only the autoinjector keypad and LED display.
This user's manual mainly describes the operation procedure for (2) and (4).
For details on procedures specific to (1) and (3), refer to the instruction manual for the operating PC
software such as GCsolution, etc. 1
 GC-2010/2010Plus/2014/2025
(1) When Using GCsolution
Figure 1-2
Complete the setting to make the autoinjector/autosampler available via the PC software.
In principle, set the parameters for the autoinjector/autosampler via the PC software.
Operate the gas chromatograph keypad only for the function settings not accessible via the
GCsolution software.
Operate the autoinjector keypad only for the function settings not available even via the gas
chromatograph.
(2) When Not Using GCsolution
Figure 1-3
In principle, set the parameters for the autoinjector/autosampler via the gas chromatograph.
Operate the autoinjector keypad for the function settings not accessible via the gas chromatograph.
Configure the AOC on the analytical flow line using the [SET] key [Line Config] on the gas
chromatograph. (Refer to the instruction manual of the gas chromatograph.)
1 Overview
 Gas Chromatographs Other than GC-2010/2010Plus/2014/2025
(3) When Using CBM-101/102 or Chromatopac GC Network
Figure 1-4
In principle, set the parameters for the autoinjector/autosampler via the computer or chromatopac.
Operate the autoinjector keypad for the functions not accessible via the operating software.
(4) In Cases Other than the Above

(E.g. GC-14A/B+C-R8A, when not using GC network)
Figure 1-5
Set all parameters via the autoinjector main unit.
@ NOTE
When GC-2010/2010Plus/2014/2025 or CBM-101/102 is not linked with AOC-20i/AOC-20s, perform the
operation procedure for (4).
1.4 Unpacking
1.4 Unpacking
Carefully inspect the condition of the shipping container before unpacking your AOC-20i/AOC-20s. If you
find any severe damage or other problems with the container, contact your Shimadzu representative
immediately.
1
After unpacking the container, verify that all items have been included by referring to the Accessories List
given in "1.5 Accessories List" P.6. If there are any missing parts or damaged items, contact your
Shimadzu representative immediately.
1 Overview
1.5 Accessories List

The autoinjector/autosampler comes with the accessories listed below. When unpacking the container,
look through the list and verify that all items have been included.
1.5.1 Autoinjector Standard Accessories

Name Part No. Q'ty Remarks
1 AOC cable S221-49794-91 1 Ferrite core on the power

supply end.
2 Rack guide (short) S221-45608-91 1 Short Rack Guide Set

S221-45610-91
3 4 ml vial rack (for solvent/waste liquid) S221-32949-01 1
4 1.5 ml vial rack S221-45609-92 1
5 Syringe (10 μl) S221-34618 1
6 Cable clamp S072-60330-01 1
7 Needle guide S221-44584 2
8 Plunger holder S221-44790 5
9 Barrel holder S221-44780 2
0 Plunger knob (Knurled screw) S037-02820 1
a Large vial (4 ml) S221-34267-91 1 pack (5 pcs.)
b Large cap (4 ml) S221-34268-91 1 pack (5 pcs.)
c Large septum (4 ml) S221-34266-91 1 pack (15 pcs.)
d Small vial (1.5 ml) S221-34272-91 1 pack (20 pcs.)
e Small cap (1.5 ml) S221-34273-91 1 pack (20 pcs.)
f Small septum (1.5 ml) S221-34239-92 1 pack (40 pcs.)
g Syringe clip S221-48989-91 1
h PAC START cable S221-35975-41 1
1.5.2 AOC Power Supply Unit

For GC-2010/2010Plus (S221-72351-41)
1 AOC power supply unit S221-72352-41 1
2 READY/START cable S221-48405-91 1 power supply unit accessories

(P/N S221-48403-91)
3 RS-232C cable for AOC S221-48406-91 1
For GC-2014 (S221-72349-41)

(P/N S221-48403-91)
1
For GC-2025 (S221-73958-91)

(P/N S221-48403-91)
For External Power Supply Unit 100 - 120 V (S221-72346-42)
1 AOC power supply unit (100 - 120 V) S221-72347-42 1
2 AC cable S071-60821-08 1 power supply unit accessories

(P/N S221-72348-92)
3 READY/START cable S221-44818-39 1
4 Stand S221-44992-92 2
5 Screw, M4 × 8 (for stand) S020-46547 4
6 Grounding wire S221-45073-91 1
For External Power Supply Unit 220 - 240 V (S221-72346-44)
1 AOC power supply unit (220 - 240 V) S221-72347-42 1
2 AC cable S071-60814-05 1 power supply unit accessories

(P/N S221-72348-94)
3 READY/START cable S221-44818-39 1
4 Stand S221-44992-92 2
5 Screw, M4 × 8 (for stand) S020-46547 4
6 Grounding wire S221-45073-91 1
1 Overview
1.5.3 Autoinjector Installation Parts

For GC-2010/2010Plus (P/N S221-44548-93) Standard Accessories
1 Spacer, rear left S221-44585 1
2 Spacer, front left S221-44892 1
3 Spacer, adjuster S221-45215 1
4 Spacer, rear right S221-48270 1
5 Spacer, front right S221-48271 1
6 Spacer S037-38786-29 1
7 Spring washer, M4 S023-77040 4
8 Washer, M4 S023-66140-01 2
9 Nut, M4 S023-04140 1
0 Wrench, 7 × 8 S086-03047-04 1
a Wrench, 5.5 × 7 S086-03047-03 1
b Screw, M4 × 30, for mounting AOC-20s S020-46554 4
For GC-2014 (P/N S221-44548-94) Standard Accessories
1 Spacer, rear S221-70185 2
6 Washer, M4 S023-66240-01 2
7 Nut, M4 S023-04140 1
8 Wrench, 7 × 8 S086-03047-04 1
9 Wrench, 5.5 × 7 S086-03047-03 1
0 Screw, M4 × 30, for mounting AOC-20s S020-46554 4
a Washer, M3 S023-77030 2
b Clamp S072-60330-01 2
c Protection sticker S221-70923 1
For GC-2025 (P/N S221-44548-95) Standard Accessories
4
Spacer, rear right
Spacer, rear left

S221-73793
S221-73794
1
1
1
6 Spacer S037-38786-29 1
8 Washer, M4 S023-66140-01 2
9 Nut, M4 S023-04140 1
0 Wrench, 7 × 8 S086-03047-04 1
a Wrench, 5.5 × 7 S086-03047-03 1
b Screw, M4 × 30, for mounting AOC-20s S020-46554 4
For GC-17A, 18A, and 1700 (P/N S221-44548-91) Optional Parts
5 INJ positioning plate, SPL S221-44785 1
6 INJ positioning plate, OCI S221-44785-01 1
7 INJ positioning plate, upper A S221-44934 1
8 INJ positioning plate, upper B S221-44935 1
9 Spacer, 2 mm, CU-402 S037-38872-21 1
0 Screw, M3 × 6 S020-46134 4
a Washer, M3 S023-66130-01 4
b Spring washer, M4 S023-77040 4
c Washer, M4 S023-66140-01 2
d Nut, M4 S023-04140 1
e Wrench, 7 × 8 S086-03047-04 1
1 Overview
For GC-14A/B, 15A, and 16A (P/N S221-44549-91) Optional Parts
4 Positioning pin S221-44725 2
5 Installation bracket S221-44731 1
6 Positioning plate (PACKED) S221-44728 1
7 Positioning plate (SPL) S221-44729 1
8 Positioning plate (OCI) S221-44730 1
9 Septum nut (PACKED) S221-44587 2
0 Septum nut (SPL) S221-44588 1
a Septum nut (OCI) S221-45204 1
b Needle guide (OCI) S221-45205 1
c Screw, M3 × 6 S020-46534 2
d Screw, M4 × 12 S020-46549 4
e Spring washer, M4 S023-77040 4
f Nut, M4 S023-04140 1
g Wrench, 7 × 8 S086-03047-04 1
1.5.4 Autosampler Standard Accessories

1 1.5 ml vial rack S221-44709-91 6
2 1.5 ml vial rack for autosampler S221-45181 1
3 4 ml vial rack
Large septum, 4 ml, 10 pcs.

S221-32949-01
S221-34266-93
2
1
1
4
5 4 ml vial, 10 pcs. S221-34267-93 1
6 Large cap, 10 pcs. S221-34268-93 1
7 Small septum S221-41233 20
8 1.5 ml vial, 20 pcs. S221-34272-91 1
9 Small cap, 20 pcs. S221-34273-91 1
0 AOC cable S221-49794-92 1 Ferrite core on both ends.
a Autosampler installation bracket S221-44486-92 1
b Autosampler installation plate for S221-44918 1

GC-17A Ver. 1
c Autosampler installation plate for S221-45168 1

GC-14B
d Spacer, CB-405 S037-38787-64 2
e Screw, M4 × 8 S020-46547 1
f Screw, M4 × 12 S020-46549 2
g Screw, M4 × 16 S020-46551 3
h Screw, M4 × 25 S020-46553 4
i Screw, M4 × 30 S020-46554 4
j Screw, M3 × 8 S221-46535 6
k Label, for rack guide S221-45094 1
l Washer, toothed lock, M4 S023-81040-01 4
m Grounding wire S221-45073-91 1
1.5.5 Autosampler Installation Parts (for GC-2014, GC-14A/B)

These parts are necessary when using the autosampler at INJ2 to 4 on GC-2014 and GC-14A/B.
Note that these are optional parts, which can be purchased as necessary.
Name Part No.

Mounting bracket for GC-2014 (for AOC-20) S221-46217-92
Long rack assembly S221-45622-91
1 Overview
1.6 Features
1.6.1 Autoinjector Features
(1) Variable injection speed
(2) High-viscosity sample aspiration available
(3) Simple and easy attachment to gas chromatographs
1.6.2 Autosampler Features

(1) Using the optional Isothermal cooling/heating vial rack, you can keep samples cool or warm.
(A circulating constant temperature bath is separately required for supplying cooling/heating water.)
1.6.3 Features Common to Autoinjector and Autosampler

(1) 1.5 ml vials and 4 ml vials are both available.
(2) Priority analysis is available by specifying a PRIORITY SAMPLE.
(3) Three different solvents can be used for washing the syringe.
(4) You can set AOC parameters via the gas chromatograph main unit (when using GC-2010/2010Plus/
2014/2025).
1.7 Specifications
1.7 Specifications
1.7.1 Autoinjector Specifications
Injection Method: Liquid sample injection via microsyringe
0.5 μl, 5 μl, 10 μl, 50 μl, and 250 μl (optional except 10 μl)
1
! CAUTION
• Be sure to use only a designated syringe. Failure to do so may damage the microsyringe,
vials, or other parts, or cause instrument malfunctions.
• The short-circuit current rating of this instrument is 50 A.
• This instrument has been designed to operate in an environment categorized as Installation

Category II and Pollution Degree 2.
• Use this instrument in locations below an altitude of 2000 meters.
• Use this instrument indoors.
Types of Vials: 1.5 ml screw cap

4 ml screw cap (optional)
Number of Samples: 6 or 12 vials (optional parts required for using 12 vials)
Syringe Speed: 2 modes: Low and high
Plunger Speed: 3 modes: Low, middle, and high
Wait Time: 0 - 99.9 sec following sample aspiration (in 0.1 sec. steps)
0 - 99.9 sec following sample injection (in 0.1 sec. steps)
Injection Volume: 0.01 - 0.50 μl in 0.01 μl steps (0.5 μl syringe)
0.1 - 4.0 μl in 0.1 μl steps (5 μl syringe)
5 - 200 μl in 5 μl steps (250 μl syringe)
Sample Wash Times: 0 - 99
Solvent Wash Times: 0 - 99
Priority Analysis: Available
Solvent: 3 different solvents are available.
Solvent Flush: Available
Power Supply: 100 V AC ± 10% (50 Hz/60 Hz), 250 VA
Dimensions, Weight: Injector Unit: 200 (W) × 78 (D) × 380 (H) (mm), 2.6 kg
External Power Supply Unit: 260 (W) × 420 (D) × 70 (H) (mm), 2.8 kg
1 Overview
1.7.2 Autosampler Specifications

Solvent Designated for Solvent Flush: Available
Standard Addition: Available
Cooling/Heating: Available when using a cooling/heating unit (optional)
(Temperature range: 0 °C to 60 °C)
Number of Samples: 1.5 ml vials: 25 vials can be set on a vial rack.
4 ml vials: 16 vials can be set on a vial rack.
Number of
1.5 ml Vials 4 ml Vials
Rack
Auto Sampler installation bracket (Standard accessory) 6 150 96
When Using the Heating 5 125 80
Optional Cooling/
Heating Unit Cooling 3 75 48
Mounting bracket for GC-2014, GC-14A/B (for AOC-20) 6 150 96

(S221-46217-92, Optional)
When Using the Heating 3 75 48
Optional Cooling/
Heating Unit Cooling 2 50 32
Cooling/Heating Unit (Optional): For 1.5 ml vials: S221-44889-91

For 4 ml vials: S221-44999-91
Standard Cooling Heating

Figure 1-6
@ NOTE
When using the Mounting bracket for GC-2014 (for AOC-20), the vial racks indicated by arrows cannot be
mounted.
Dimensions, Weight: 320 (dia.) × 135 (H) mm, 2.4 kg
1.8 Part Names
1.8 Part Names

1.8.1 Autoinjector
1
1
9 4
7
6
Figure 1-7
No. Name No. Name
1 Case 6 Rack guide
2 Door 7 Rack gear
3 Plunger drive unit 8 Keyboard unit
4 Syringe drive unit 9 Display
5 Needle guide
1 Overview
1.8.2 Autosampler
Figure 1-8
No. Name No. Name
1 Arm 4 Tray
2 Gripper 5 Power/status indicator
3 Vial rack
1.8 Part Names
1.8.3 Power Supply Unit
 Built-in Power Type

The power supply unit is located at the left bottom on the gas chromatograph back panel.
! WARNING
1
• HIGH VOLTAGE
Risk of electric shock. Only authorized Shimadzu service personnel can remove the cover.
1 1
1
2 2
2
3 3
3
4 4 4
5 5 5
6 6 6
Figure 1-9 For GC-2010/2010Plus Figure 1-10 For GC-2014 Figure 1-11 For GC-2025
No. Name No. Name
1 START/READY terminal 4 Autosampler control terminal
2 Fiber optic link terminal 5 Injector 2 control terminal
3 RS-232C terminal 6 Injector 1 control terminal
Normally, No. 2 Fiber optic link terminal is not used.
1 Overview
 External Power Type
! WARNING
• Be sure to use only a fuse of the specified type and rating.
Be sure to unplug the power supply cable before replacing a fuse.
Figure 1-12
No. Name No. Name
1 Power indicator 2 Power switch
9 8 7 6 5 4 3 2
Figure 1-13
No. Name No. Name
1 AC Power inlet and Fuse holder 6 Aux control terminal
2 Ground terminal 7 Autosampler control terminal
3 START/READY terminal 8 Injector 2 control terminal
4 Fiber optic link terminal 9 Injector 1 control terminal
5 RS-232C terminal
1.9 Operation Environment
1.9 Operation Environment

To maintain the instrument performance, always use the instrument within the specified temperature/
humidity ranges described below. This instrument can operate within the operating temperature/humidity
ranges described below.
Specified Temperature Range: 10 °C - 30 °C 1

Specified Humidity Range: 50% - 60%
Operating Temperature Range: 5 °C - 40 °C
Operating Humidity Range: 5% - 90%
1 Overview
2 2 Autoinjector/
Autosampler Installation
2.1 Installing the Autoinjector to the Gas 2

Chromatograph
2
2.1.1 Installation to the Gas Chromatograph
When installing the autoinjector to the gas chromatograph, you can use the uniform procedure described
below regardless of your gas chromatograph model. 2
The following explanation uses GC-2010 as an example in Figures.
2
1 Place the autoinjector directly upon the four installation pins located on top of the gas
chromatograph. Press the autoinjector down until it is set onto the septum nut and
2
completely secured.
Then, plug in the AOC cable to connect the AOC to the GC, if it is unplugged.
! CAUTION
2
• Be sure to connect/remove the AOC cable when the AOC power is OFF.
Connecting/removing the cable while the AOC power is ON will result in instrument malfunction 2
or failure.
1 Place directly upon the installation pins until the autoinjector is completely secured. 2
2 AOC cable: Align the parts indicated by arrows to plug/unplug the cable.
3 Place the AOC cable on the hook (accessory) to avoid interrupting the rack movement.
2
1
2
2
2
2
3
2
2
Figure 2-1
2
2
@ NOTE
If the autoinjector is not completely secured, the error message "-11" appears on the display when
the power is turned ON. In this case, check whether the autoinjector is properly mounted, and press
it thoroughly until it is securely fixed.
@ NOTE
Even though the AOC cable is connected, you can install/remove the autoinjector. Remove the AOC
cable when moving the autoinjector to places where the AOC cable cannot reach (e.g. when
carrying the autoinjector away).
To remove the autoinjector, pull it straight upwards.
@ NOTE
If the autoinjector is used for a GC-2014 or GC-14A/B, Shimadzu personnel remove at installation
the plate spring, screws, and spacers (shown in Figure 2-2) used for mounting to GC-2010 or other
gas chromatographs. These parts are necessary when using the autoinjector with GC-2010, etc.
Please store them in a safe place.
The plate spring, screws, and The hole for the septum nut
spacers here have been
removed. (They are required
for a GC-2010, GC-17A or
other chromatographs.) Bottom view of autoinjector
Figure 2-2
2.1 Installing the Autoinjector to the Gas Chromatograph
2.1.2 Mounting Spacers on GC-2014/GC-14A/B

For GC-2014, the spacers (autoinjector installation parts) are mounted to the positions described below,
according to the installation position of the autoinjector. (The positions of the spacer are also the same for
GC-14A/B even though the front and rear spacers are fixed to the mounting plate.)
Spacer, Rear
S221-70185
1 2 3 4
Spacer, Front Left

S221-44892
Spacer, Front Right
1 2 1 3 2 4 3 4 S221-70183
Figure 2-3
When installing the autoinjector on GC-2014 and GC-14A/B, you can mount up to two sets of the spacers
simultaneously. The positions can be selected from the following combinations.
(1 and 2), (2 and 3), (3 and 4), and (1 and 4)
To install the autoinjector to the injection port with no spacer mounted, move the spacers by the following
procedure:
For GC-2014
Rear Front Front

INJ/DET cover side left side right side
(GC rear side) (GC front

side)
M3 Spring washer
M4 Spring
washer
INJ/DET mounting plate
(GC-2014 viewed from left side) Spacer shapes
Figure 2-4
The front left spacer is an assembly of Spacer, front left bottom (S221-44892), Nut (S023-04140), and
Spacer, adjuster (S221-70184).
1 Turn OFF the power of GC-2014.
2 Loosen the knurled screw fixing the INJ/DET cover, and remove the cover.
3 Using the wrench (accessory), remove two of the front spacers mounted to the INJ/DET
mounting plate. Remount them to the designated positions by referring to Figure 2-3.
4 Using the wrench, remove two of the rear spacers mounted to the INJ/DET mounting
plate. Remount them to the designated positions by referring to Figure 2-3.
5 Reinstall the INJ/DET cover to the original position, and tighten the knurled screw to fix
the cover.
@ NOTE
• Be careful not to lose the spring washers, which have been set with spacers.
• Pay extra attention not to confuse the right and left front spacers.
• If the autoinjector is unstable when it is installed, open the oven door and adjust the length of the front left
spacer.
Loosening the nut in the middle of the front left spacer allows you to adjust its height of the spacer top.
After completing the adjustment, tighten the nut to fix the spacer top securely.
2.1 Installing the Autoinjector to the Gas Chromatograph
For GC-14A/B
Perform procedures similar to that for GC-2014 to mount spacers designated in the Figure 2-5 below.
Spacer, Rear (S221-44727)

Fix the spacer with M4 spring washer set in between.
M4 Spring washer
M4 Spring washer
2
Spacer, Front Left (Height
Adjuster) (S221-44726)
Adjust the spacer height when
the autoinjector is unstable after
installation. Fix with the M4 nut.
(Use the accessory wrench.)
M4 Spring washer Spacer, Front Right (S221-44895)

Fix the spacer with M4 spring washer set in
between.
M4 Nut
Figure 2-5
2.2 Mounting and Removing the Syringe

The 10 μl syringe (P/N S221-34618) is supplied with the autoinjector as a standard accessory.
This section describes the procedures for mounting/removing the microsyringe.
Use the same procedures for syringes with different volumes (optional). In this case, see also the
description in "3.3.3 Injection Modes", "Large Volume Injection Mode (Low-speed Injection Method)" P.115
to complete the required parameter change.
2.2.1 Mounting the Microsyringe
1 Turn ON the power of the autoinjector.

(For the power ON procedure, see "3.1.2 Turning the Power ON/OFF" P.80.)
2 Hook your finger at the half-circle depression on the autoinjector top, and pull the door
open.
@ NOTE
Check that "OP" appears on the display when the door is open. If it does not appear, contact your
Shimadzu representative.
3 Press the [STOP] key while the door is open.

(The plunger motor power is turned OFF, and the autoinjector becomes ready for the microsyringe to be
mounted.)
Half-circle depression on the top
[STOP] key
Figure 2-6
4 Inspect the new syringe. Check if the needle is fixed to the syringe barrel in a straight
manner.
You can check the straightness of the needle by rolling the syringe on a flat surface (Avoid the flange
interference). If the needle is set at an angle, the distance to the needle tip from the surface keeps
changing during the rotation.
If you mount the microsyringe with an angled needle to the autoinjector, the needle may be bent during
the injection operation.
When the needle is set at an angle, straighten the needle by slightly bending from the needle base (i.e.,
where the needle is fixed to the syringe barrel) with your fingers.
2
5 Attach the barrel holder (used for syringe removal: P/N S221-44780) onto the syringe
barrel.
The barrel holder should be positioned within the range shown in Figure 2-7.
1 Slide the barrel holder in the direction shown.

2 Position the barrel holder 4 to 6 mm from the flange.
3 Slide the barrel holder against the flange in the direction shown in the figure below at right.
4 Attach the barrel holder so that its protruded part comes to the graduated side of the syringe.
1 Flange
Barrel Barrel holder Protrusion side

4
2 4 - 6 mm
Barrel holder Flange
3
The syringe is positioned correctly
when the graduations are visible.
Figure 2-7
6 Lift the syringe drive unit to its highest position. Pull up the plunger drive unit at least
16 mm by holding the knurled screw.
If it is difficult to pull up the plunger drive unit, rotate the plunger motor pulley by hand.
1 Lift the syringe drive unit to its highest position.

2 With the power ON and the initial operations completed, the syringe drive unit is in its highest
position (home position).
Knurled screw
Plunger motor pulley
Plunger drive unit

1, 2
Lift up at
least 16 mm.
Syringe drive unit
Figure 2-8
@ NOTE
Until doing procedure 3 (pressing the [STOP] key while the door is open), you cannot lift up the
plunger drive unit since the plunger motor power is still ON. Be sure to complete procedure 3 before
proceeding.
7 Open the syringe clip while the syringe drive unit is located at its highest position.
1 Open the syringe clip.
8 Press the syringe plunger down to its lowest position. Tilt the syringe top toward you
and insert the needle tip to the needle guide in an oblique manner.
At this point, the scale marks on the syringe barrel should be directed toward you.
1 Position the protrusion side of the barrel holder to face outward.

2 Insert the needle into the needle guide by 2 mm.
3 Push the barrel into the clips of the syringe drive unit until it is fixed.
9 Set the syringe barrel all the way until the clips of the syringe drive unit hold the barrel
securely. Make sure that the plunger flange is fit on Clip 3.
If the barrel is not properly set here, the needle may be bent during operation.
Verify that the barrel holder is located in the position specified in Figure 2-9, and that the needle tip is
inserted into the needle guide.
1 Place the long side of the flange toward you.

2 Insert the flange above clip 3.
3 Position the barrel holder between clips 2 and 3.
4
2
Ensure that the needle is in the needle guide.
5 The syringe graduation marks should be visible.
The syringe will be positioned as in the Figure 2-9 if the barrel holder is attached as shown in Figure
2-7.
10 Fix the syringe barrel with the syringe clip.

Secure the syringe with the syringe cap.
9-1, 9-2
Clip 3
9-3
Clip 3
Clip 2
Clip 2
8-1 10
7
Clip 1
8-3 Clip 1
Needle guide
9-5
8-2 9-4
Left side view Front view
Figure 2-9
@ NOTE
With the barrel holder and syringe flange placed in the direction specified in Figure 2-9, install the
syringe to the syringe drive unit until it is completely secured. If the placement direction is not correct
or the syringe is not mounted securely, the needle may be bent during operation.
@ NOTE
Be sure to close the syringe clip before operation. If the syringe drive unit operates with the syringe
clip opened, the clip may fall off or be broken.
An extra syringe clip is supplied with the autoinjector as a standard accessory. To install the new
syringe clip, follow the instructions in Figure 2-10.
Syringe clip
(P/N S221-48989-91)
To install the syringe clip, push the clip up and down

with your fingers in the directions of arrows.
Figure 2-10
@ NOTE
Before mounting the syringe, be sure to press down the syringe plunger to its lowest position. If the
plunger is not completely pressed down, it may be bent by being caught on the plunger drive unit.
11 Verify that the syringe is securely set to the syringe drive unit, and the syringe plunger
pressed down to its lowest position. Attach the plunger holder to the plunger button.
Be sure to insert the holder all the way until it contacts the plunger button.
Plunger holder
Plunger button 2
Slide the plunger

holder onto the
plunger button from
the front.
Confirm that the

plunger is all the way
down, and then slide
the plunger holder onto
the plunger button.
Figure 2-11
12 Loosen the knurled screw on the plunger drive unit to create a gap of 7 mm or longer
between the screw and the unit.
@ NOTE
Loosen the knurled screw on the plunger drive unit sufficiently. If the top of the plunger holder
contacts the screw, you cannot fasten the screw properly and the plunger may be damaged.
Pull out of the hole.
Hole
Knurled screw
Pull out by 7 mm or more.
Figure 2-12
13 Pull down the plunger drive unit, which was lifted up 16 mm in procedure 6, until its
bottom hole is set on the plunger holder top.
To pull down, place your fingers on the upper part of the plunger drive unit or the knurled screw. If it is
hard to pull down, rotate the pulley of the plunger motor by hand.
1 Lower the plunger drive unit.

2 The top of the plunger holder fits into hole.
Upper part of plunger

drive unit
Hole
1
Knurled screw
Top of plunger holder 2
Figure 2-13
14 Press the [RESET] key (Orange).

The plunger drive unit moves to its home position.
Plunger drive unit
Moves downward.
Small clearance
should be found.
Figure 2-14
! CAUTION
• DO NOT place your hand near the drive unit while the plunger drive unit is operating.
The plunger drive unit will then stop, leaving a small gap between it and the syringe drive unit.
15 Lightly press down the plunger holder so that the syringe plunger tip contacts the
syringe barrel bottom.
Check to be sure that the plunger tip is located at the barrel zero point.
Knurled screw
Figure 2-15
16 Tighten the knurled screw on the plunger drive unit to fix the plunger holder.
@ NOTE
The plunger tip should already be in contact with the barrel bottom and located at the zero point
upon completing procedure 8. Lightly press down the plunger holder hook with your fingers, and
tighten the knurled screw securely.
DO NOT press down the plunger holder too strongly as you may bend the plunger.
Confirm that the plunger

touches the bottom.
Figure 2-16
@ NOTE
After fixing the plunger, make sure that the plunger tip is in contact with the syringe barrel bottom
(i.e., the plunger tip is located at the zero point of the barrel). When the plunger is not in contact with
the barrel bottom, replace the plunger holder and follow the syringe mounting procedure from the
beginning.
Verify that there is a small gap between the plunger drive unit and the syringe drive unit as shown in
Figure 2-16, illustrating the plunger drive unit in its home position.
! CAUTION
• Always turn ON the instrument before mounting the microsyringe. If the power is OFF,
the plunger drive unit cannot move to its home position, which may cause improper
sample aspiration.
17 Close the autoinjector door.
This completes the installation of the microsyringe to the autoinjector.

For the procedures for injecting samples, see "3 Operation" P.79.
@ NOTE
Once the plunger holder is used, the knurled screw creates a small dent in it. Therefore, it is recommended
to replace the plunger holder when replacing the microsyringe.
@ NOTE
The plunger motion becomes constricted after repeated air aspiration/discharge, or when the syringe is left
unused for a long time. Before mounting the microsyringe to the autoinjector, check to be sure that the
plunger moves smoothly. Additionally, always maintain the plunger in good condition by rinsing it with
solvents, etc.
If the plunger motion is too constricted, the autoinjector aborts the operation, displaying the error "-03". To
avoid this, always maintain the plunger in good condition.
The motion of 50 μl syringes and 250 μl syringes (both optional) especially become constricted in shorter
period of time than the standard 10 μl syringes. After using these syringes, be sure to perform maintenance
on them immediately.
@ NOTE
Remember that syringes are consumables. The plunger and barrel become loose after long periods of use,
which may cause sample leaks. The sample leaks will deteriorate analysis reproducibility. Periodically check
that there is no leak when replacing the gas chromatograph injection port septum, etc.
! CAUTION
• OCI Syringe (for only when using GC-17A (OCI-17))
Use the OCI syringe (P/N S221-37282-02: optional) when using the OCI as an injection port.
The needle of the OCI syringe thins towards its tip, allowing the introduction of samples directly to the
capillary column. To set the OCI syringe to the autoinjector, use the same procedure as that for other
syringes, except set the syringe injection position 3 mm higher than usual. If you use the autoinjector
without the parameter change, you may break your syringe barrel.
When using a standard syringe again, be sure to reset to the original value, 0 mm. If you keep using
the autoinjector with the injection position 3 mm higher, it may deteriorate analysis reproducibility.
2
1 Press the [FUNCTION] key.
(The FUNCTION number set in the last operation is displayed.)
2 Access F27 using the arrow keys, and press the [ENTER] key.
3 Change the displayed value to "003", and press the [ENTER] key.
@ NOTE
This parameter can be set within the range from 0 up to 22 mm (upward direction).
2.2.2 Removing the Microsyringe
1 If the autoinjector power is ON, press the [RESET] key to lift up the syringe drive unit
and to return the plunger to its home position.
2 Open the autoinjector door, and press the [STOP] key.
Figure 2-17
If the autoinjector power is OFF, open the door and verify that the syringe drive unit is located at its
highest position. If not, lift the syringe drive unit up to its highest position with your fingers.
Figure 2-18
3 Loosen the knurled screw fixing the plunger, and lift up the plunger drive unit 16 mm or
more.
If it is difficult to pull up the plunger drive unit, rotate the plunger motor pulley by hand. When the AOC
power is OFF, even though the plunger drive unit is not located in its home (lowest) position, you can
remove the syringe by lifting the plunger drive unit up 16 mm or more from the current unit position.
Loosen the knurled screw,

and lift the plunger drive
unit until the plunger
holder is unfastened.
Top of plunger
holder
Figure 2-19
4 Remove the plunger holder from the plunger. Then, open the syringe clip.
5 Verify that the plunger is fully pressed down to its lowest position. Hold the barrel
holder and tilt the barrel down toward you to remove the syringe from the syringe drive
unit.
Barrel holder
Figure 2-20
This completes the removal of the microsyringe from the autoinjector.
2.3 Setting Vial Racks to the Rack Guide, Loading Rack

into the Autoinjector
2.3.1 Setting Vial Racks to the Short Rack Guide

The rack guide and vial rack, as shown in Figure 2-21, are separate units which are combined according to
the application requirements.
1 Rack 2 4 ml vial rack 3 1.5 ml vial rack

(for solvent/waste liquid)
Figure 2-21
The standard accessories are: 1 short rack guide (S221-45608-91), 2 4 ml vial rack (S221-32949-01)
and 3 1.5 ml vial rack (S221-45609-92) without sockets. The short rack guide accommodates 6 vials.
To load the vial racks, spread the rack guide slightly apart and insert both 3 1.5 ml vial rack and 2 4 ml
vial rack, so that the side pegs fit into the corresponding holes in each side of 1 rack (as shown in Figure
2-22).
Figure 2-22
2.3 Setting Vial Racks to the Rack Guide, Loading Rack into the Autoinjector
2 and 3 are positioned as shown in Figure 2-23 at shipment.
Figure 2-23
2.3.2 Setting Vial Racks to the Long Rack Guide (Optional)

The long rack guide and vial racks, shown in Figure 2-24, are separate units which are combined
according to the application requirements.
2
1 Long rack 2 4 ml vial rack 3 1.5 ml vial rack (without sockets)

(for solvent/waste liquid) 3' 1.5 ml vial rack (with sockets)
Figure 2-24
To assemble the long vial racks, first connect two 1.5 ml vial racks, 3 and 3' end-to-end, by inserting the
pegs of 3 vial rack into the sockets of 3' vial rack.
Sockets
3 1.5 ml vial rack (without sockets)
3' 1.5 ml vial rack (with sockets)
Pins
Figure 2-25
The assembled vial racks are inserted into the long rack guide along with 2 4 ml vial rack (for solvent/
waste liquid vials), as shown in Figure 2-26. This is the configuration at shipment.
3' 3 2
Figure 2-26
As for the short rack guide, the pegs of 2, 3, and 3', fit into the sockets of 1 long rack guide. Spread the
rack guide apart slightly, and insert the vial racks.
3'
Figure 2-27
2.3.3 Loading Rack into the Autoinjector

Use the same procedure to load both the long and short rack guides into the autoinjector. However, the
length of the rack guide used must be specified via the gas chromatograph, according to the procedure
described below.
@ NOTE
When using PC software such as GCsolution, be sure to perform this setting via the PC software. Otherwise,
the set values will be erased. For details, refer to the system configuration section of the software instruction
manual.
For GC-2010/2010Plus/2014/2025 2
1 Press the [OPTION] key on the gas chromatograph to display the [AOC Parameters] window.
2 Press [OtherPara (PF3)] of the PF menus to display the [AOC Other Parameters] window.
3 Change the rack guide setting according to the type of rack guide used.
Figure 2-28
@ NOTE
When using GC-2010/2010Plus/2014/2025, be sure to change the parameters via the gas chromatograph.
DO NOT change the parameters by operating the autoinjector keypad, because when rebooting the
instrument all parameters on the autoinjector are updated to the AOC parameters set on the gas
chromatograph.
For details on the gas chromatograph operation, refer to the instruction manual of the gas chromatograph.
@ NOTE
When the [AOC Parameters] window does not appear even if you press the [OPTION] key many times,
press the [SET] key on the gas chromatograph and select [LineConfg] to check if the AOC is specified for the
current analytical flow line.
For Other Gas Chromatographs

3 Change the displayed value as follows, and press the [ENTER] key.
Short rack guide: "000" (default)
Long rack guide: "001"
This completes the long/short setting for rack guides to be used.
Follow the procedure below to install the rack guide and racks into the autoinjector.
1 The autoinjector power can be ON or OFF. Verify that the syringe drive unit is at its
highest position.
If it is not all the way up, it will strike the rack when it is inserted. Turn OFF the power and be sure to lift up
the syringe drive unit to its highest position.
Figure 2-29
2 Insert the rack guide into the autoinjector track so that the rack gears catch on the
motor pinion.
Once the gears catch, insert the rack guide a further 20 mm. The rack guide can be inserted from the left
or right if no autosampler is installed. If an autosampler is in use, insert the rack guide from the right.
Figure 2-30
3 When the autoinjector power is turned ON, the rack moves to the home position (all the
way to the left) as shown in Figure 2-31.
If the power is already ON, press the [RESET] key to move the rack to the home position.
Figure 2-31
@ NOTE
When the rack is in the home position, the motor is released, and the rack can be removed.
@ NOTE
During analysis, when the GC is in the READY state, the motor is engaged. The rack can still be removed
and inserted without pressing the [RESET] key; however, this will be more difficult because the motor has
not been released. Remove the rack, if necessary, then re-insert as in step (2). Press the [START] key once
to begin the analysis.
If the [RESET] key is pressed before removing or after inserting the rack, analysis will not be performed
continuously. Once the [RESET] key is pressed, pressing the [START] key begins a new analysis, not a
continued analysis.
2.3.4 Washing with 3 Different Solvents

By adding two of 4 ml vial racks, you can wash the syringe with 3 different solvents.
In this case, mount the racks as shown in the figure below. The short rack guide can measure a 1.5 ml
sample vial, and the long rack guide can measure seven of the 1.5 ml sample vials. The position indicated
with "1" is designated for 4 ml vials.
Positions for sample vials 1
C Waste
(Positions for sample vials) liquid vial
B Waste
liquid vial
A Waste
liquid vial
Solvent Vial Solvent Vial
Solvent Vial
2
1
Figure 2-32
For GC-2010/2010Plus/2014/2025
1 Press the [OPTION] key on the gas chromatograph to display the [AOC Parameters]
window.
2 Press [OtherPara (PF3)] of the PF menus to display the [AOC Other Parameters]
window.
3 Set [Using 3 Solvent Vials] to [Yes].
4 Set [Solvent Select] to [All]. (Alternatively, select one out of 3 solvents.)

For details on the gas chromatograph operation, refer to the instruction manual of the gas
chromatograph.
Figure 2-33
1 Press the [FUNCTION] key on the autoinjector main unit.

3 Change the displayed value to "001", and press the [ENTER] key.
The default value is "000" (Use one solvent).
@ NOTE
Compared to the solvent flush mode using the autosampler, this function has the following differences.
• You cannot add standard samples.
• You cannot use the solvent for solvent flush method in a designated vial.
• The wash solvent is used as the solvent for solvent flush method. (The 3rd wash solvent when three
solvents are used)
@ NOTE
When FUNCTION 08 is set to the initial value ("000"), three solvents can be used (see "3.2.8 FUNCTION
Key" P.92, Solvent select F08).
2.4 Vial Types and Vial Mounting Procedure on Rack Guide
2.4 Vial Types and Vial Mounting Procedure on Rack

Guide
The rack can be loaded with vials either on or off the autoinjector.
2.4.1 Vial Types

The autoinjector can accommodate the two vial sizes shown in Table 2-1.
Table 2-1 2
Type Part No. Use
Small vial S228-16550 Sample/standard
1.5 ml
Large vial S228-21281-01 Solvent/Waste liquid
4 ml
@ NOTE
Use the specified vials only. Use the vials for their intended purpose. 4 ml vial can be used for samples. But
optional parts are required. See "2.7.3 Using 4 ml Vials (Option)" P.70.
Be sure to use the septa and caps listed in Table 2-2 according to the vial size.
Table 2-2
CAP TYPES
Type Part No. Color

Small cap S221-41242 White
For 1.5 ml sample vial
Large cap S221-39951 White
For 4 ml vial
SEPTUM TYPES
Type Part No. Remarks

Small septum S221-41233 PTFE-coated silicon
For 1.5 ml sample vial
Large septum S221-15324
For 4 ml vial
Concerning the part numbers for above items, see "1.5 Accessories List" P.6.
! CAUTION
• The vial caps for use with the autoinjector must be white. If the vials have no caps or have
caps of another color, the autoinjector may not recognize the vials. If a part other than that
specified is used, the sensor will be unable to detect it, resulting in possible damage. Use
only the specified caps.
2.4.2 Septum Replacement and Sample Volume in Vials

Securely tighten the cap over the septum, or the sample may evaporate.
! CAUTION
• A loose cap could damage the unit.
@ NOTE
Pay attention to the septum direction. The PTFE side must be directed downwards.
Cap
Septum
PTFE surface down
Vial
Figure 2-34
Throughout this manual, polytetrafluoroethylene is abbreviated PTFE.
Replace septa frequently. The effectiveness of the septum seal deteriorates over time, so avoid using the
same septum repeatedly. Also, once the septum has been punctured, certain solvents can deteriorate the
septum and release contaminants into the sample.
The minimum amount of sample needed in a vial is determined by the length of the syringe needle, which
is 43 mm. At least approximately 3.5 mm of solvent is required for a 1.5 ml vial, as shown in Figure 2-35.
For a 4 ml vial, a minimum of approx. 7.5 mm is required.
approx. 7.5 mm
approx. 3.5 mm
1.5 ml vial 4 ml vial
Figure 2-35
 Changing the Depth of the Syringe Needle when Inserted into Sample Vials
Reducing the depth of the syringe needle when inserted into sample vials
• For GC-2010/2010Plus/2014/2025
Press the [SET] key on the gas chromatograph and select [LineConfg] to check if the AOC is specified
for the current analytical flow line.
2
3
Press [OtherPara (PF3)] of the PF menus to display the [AOC Other Parameters] window.
Set [Syringe Height (↑)] in millimeter (setting range: 0 - 20 mm). 2
4 Set [Syringe Height (↓)] to [0].
Figure 2-36
• For Other Gas Chromatographs

Change the parameter of FUNCTION 25 on the autoinjector.
"000" - "020" (0 - 20 mm) .......... Default "000"
FUNCTION 26 .......................... Leave as default value ("000").
Increasing the depth of the syringe needle when inserted into sample vials
To increase the depth of the syringe needle when inserted into vials with small sample volume, perform the
following procedure according to the vial types.
(1) When using 1.5 ml vials
For GC-2010/2010Plus/2014/2025
3 Set [Kinds of Vials] to [1.5 ml] (default).
4 Set [Syringe Height (↓)] in millimeter (setting range: 0 - 20 mm, default value: 0).
5 Set [Syringe Height (↑)] to [0].
Figure 2-37

Set the parameters as follows.
FUNCTION 29........ "000" (Vial type 1.5 ml, default value "000")
FUNCTION 26........ "000" - "002" (setting range: 0 - 2 mm, default value "000")
FUNCTION 25........ Leave as default value ("000").
(2) When using 4 ml vials

Besides vial racks for solvents/waste liquid, prepare another 4 ml vial rack (S221-32949-01)
separately.
You can set two 4 ml sample vials on the vial rack. See "2.3 Setting Vial Racks to the Rack Guide,
Loading Rack into the Autoinjector" P.38 for the procedure to mount the vial rack to the rack guide.
Complete the following settings to aspirate larger amounts of samples in the vials, since 4 ml vials
are deeper than 1.5 ml vials.
For GC-2010/2010Plus/2014/2025
1
2
Press the [OPTION] key on the gas chromatograph to display the [AOC Parameters] window.
Press [OtherPara (PF3)] of the PF menus to display the [AOC Other Parameters] window.
2
3 Set [Kinds of Vials] to [4 ml].
4 Set [Syringe Height (↓)] in millimeter (setting range: 0 - 10 mm).
Figure 2-38

FUNCTION 29........ "001" (Vial type: 4 ml)
FUNCTION 26........ "000" - "010" (setting range: 0 - 10 mm)
FUNCTION 25........ Leave as default value ("000").
To change 4 ml vials to 1.5 ml vials, complete the parameter setting described below. If this is not
done, the needle will hit the 1.5 vial bottom, breaking both the needle and vials.
For GC-2010/2010Plus/2014/2025
3 Set [Kinds of Vials] to [1.5 ml].
4 Set [Syringe Height (↓)] to [0].
Figure 2-39
chromatograph.

FUNCTION 29........ "000" (Vial type: 1.5 ml)
FUNCTION 26........ "000" (set to 0 mm)
Maximum number of sample injections
The number of injections is limited by the capacity of the 4 ml rinse solvent and waste liquid vials. Assume
that a 10 μl syringe is used, with aspiration of 8 μl per wash.
The rinse solvent vial volume limit refers to the maximum number of sample injections possible before a full
solvent vial (4 ml) is emptied by aspirations.
For example, a maximum of 125 injections can be made when specifying 3 solvent washes (as indicated
by dashed line (Graph 2-1)). Assuming 125 injections, 3 × 125, or 375 solvent washes will be performed.
This will use all of the available solvent; even if more solvent washes are attempted, no solvent will be
aspirated. (The actual solvent vial volume limit will be less than the amount indicated here due to loss by
evaporation according to the solvent type.) 2
The other factor that must be considered is the waste liquid vial volume limit. This refers to the number of
sample injections possible before the waste liquid vial overflows. Since the autoinjector performs sample
washes and solvent washes, this limit value indicates the sum of both wash times.
There are several possible combinations of solvent and sample washes which yield a total of 3 washes, as
shown in Table 2-3.
Table 2-3
Frequency
Sample Wash 0 1 2 3
Solvent Wash 3 2 1 0
Use the graph to help determine whether the limiting factor is the solvent vial volume or the waste liquid
vial capacity. Use the smallest number of injections indicated by the graph.
Possible # of injections
Was
te liq
uid vial c
apac
ity limit
Solv
ent v
ial vo
lu me li
m it
No. of washes per injection

Graph 2-1
! Warning
• USE CARE WHEN HANDLING FLAMMABLE SOLVENTS OR SAMPLES
To prevent flammable solvents or samples (such as carbon disulfide) from evaporating and filling the
installation site, securely tighten the cap that holds the septum in place on all vials (including rinse
solvent and waste liquid vials).
@ NOTE
Installation Precautions
Be sure to use the optional sample cooling fan when working with solvents with particularly strong
flammability, such as carbon disulfide.
For other flammable solvents or samples, it is also recommended to use the sample cooling fan.
• Prevent spills by never exceeding the waste liquid vial capacity.

If the waste liquid vial overflows or the vapor fills the installation site, the heat from the injection port
could ignite the liquid or vapor, causing a fire.
2.4.3 Vial Placement

In this section, the short rack introduced in "2.3 Setting Vial Racks to the Rack Guide, Loading Rack into
the Autoinjector" P.38 is described in greater detail.
As shown in Figure 2-40, the rack guide and vial racks are separate components.
1 Rack Guide 2 4 ml vial rack 3 1.5 ml vial rack

(for solvent/waste liquid vials)
Figure 2-40
See Figure 2-23 for the assembly of 2 and 3. Figure 2-41 shows the vial arrangement in the assembled
rack.
3 1.5 ml vials (6 possible positions) 2 1
4 ml solvent vial position

4 ml waste liquid vial position
Figure 2-41
1.5 ml vials can be placed in any position of 3. Injection starts from the vial closest to the injection port,
and proceeds in numeric order, as shown in Figure 2-42.
@ NOTE
Injection can proceed in an arbitrary order when performing an analysis schedule on GC-2010/2010Plus/
2014/2025 or batch analysis on GCsolution, CLASS-GC10, and Chromatopac GC net.
2
Injection port
Figure 2-42
Example: : Positions where a vial is located

: Positions with no vial
Figure 2-43
Place the samples in any position. The injection starts from the position closest to the injection port (1 → 2
→ 3 in the example above).
@ NOTE
The vial mounting procedure and injection order is the same for the long rack, except that there are more
1.5 ml vial positions (12).
@ NOTE
Ensure that a waste liquid vial is in place. If not, a "-14" error message appears.
2.5 Replacing INJ Port Septum
! WARNING
• DO NOT perform septum replacement before the INJ cools down to 50 °C or lower.
Attempting replacement work when the INJ is still hot will cause burn injuries.
! CAUTION 2
• To prevent the screw part from being burnt, DO NOT tighten the screw and nut when
the INJ is still hot.
The injection port septa are durable for only approximately 100 injections. Exceeding this number of
injections may cause gas leaks and deteriorate analysis reproducibility. Be sure to replace the septum
before it exceeds its service life.
@ NOTE
By setting the analysis counter function on GC-2010/2010Plus/2014/2025, GC-17A Ver.2,3, 1700, and 18A,
a message informing you of the septum replacement time is displayed on the GC screen. For details, refer to
the instruction manual of the gas chromatograph.
Remove the autoinjector and replace the injection port septum by the procedures described below. They
are basically the same regardless of the gas chromatograph type, except for procedure 1.
1 Turn OFF the power of the autoinjector.
For GC-2010/2010Plus/2014/2025
If the gas chromatograph power is ON, switch OFF the autoinjector as follows:
2 Set [AOC POWER] to [Off], and press the [ENTER] key.
Figure 2-44
1 Turn OFF the power switch on the power supply unit.
2 Open the autoinjector door, and verify that the syringe drive unit is located at its highest
position.
If not, lift up the syringe drive unit as shown in Figure 2-45.
Lift up the syringe drive unit to its

highest position.
Figure 2-45
! CAUTION
• If the autoinjector is removed when the syringe drive unit is not at its highest position,
the syringe needle may be broken or damaged.
3 Lift the autoinjector straight up and set it aside during septum replacement. (e.g. on the
back of the INJ/DET cover in the case of GC-2010/2010Plus or GC-17A)
Connection terminal
Figure 2-46
By unplugging the cable from the terminal, you can move the autoinjector to a place where the cable
cannot reach.
4 Replace the injection port septum.

(For the detailed procedure, refer to the instruction manual of the gas chromatograph.)
@ NOTE
Be sure to use the needle guide as well as septum nut designated for the autoinjector.
(They are standard accessories for GC-2010/2010Plus/2014/2025.)
When using GC-14A/B, 15A, and 16A, pay extra attention to the shape of the septum nut. Differently
shaped septum nut must be used according to the injection port types.
Note that the OCI-14 needle guide is designated only for OCI-14, unlike those for the other injection
ports. This needle guide is longer than others. Figure 2-47 below shows the shapes and dimensions
of various autoinjector needle guides and septum nut.
GC-14A/B septum nut for packed or septum

purge injection port
φ4.9
φ24.8
φ24.8
5.0
Autoinjector needle guide, standard 1.5 3.0

accessory (same for GC-14A/B, -15A,
-16A, and -17A). Not for use with OCI-14. 11.5 7.5
Septum nut of packed INJ, GC-14A/B septum

INJ with septum purge for nut for SPL-14
GC-14A/B (split/splitless)
φ4.9
φ24.8
12
GC-14A/B needle guide for GC-14A/B septum nut for

10.5
OCI-14 OCI-14
16.5
Figure 2-47
@ NOTE
In the case of GC-14A/B, 15A, or 16A:
When there are other INJ ports next to the one currently used (e.g. DUAL packed INJ), they also
must have the septum nut and needle guide designated for the autoinjector.
If standard items (i.e., not those designated for the autoinjector) are mounted to the other INJ ports,
the septum nut may obstruct the proper installation of the autoinjector due to its height.
You can install the autoinjector by removing the septum nut and needle guide, if those INJ ports are
not used.
Note that the standard septum nut for GC-2014 does not obstruct the autoinjector installation.
INJ unit to be used
Autoinjector
Replace these septum nuts and needle guides
with those for the autoinjector.
Figure 2-48
5 Following the installation procedure described in "2.1 Installing the Autoinjector to the
Gas Chromatograph" P.21, reinstall the autoinjector to the original position.
6 If you turned OFF the AOC power via GC-2010/2010Plus/2014/2025 in procedure 1, turn
ON the autoinjector as follows.
2 Set [AOC POWER] to [On], and press the [ENTER] key.
Figure 2-49
chromatograph.
2.6 Available Options

This section explains about the optional parts attaching to the autoinjector main unit.
2.6.1 Sample Cooling Fan (P/N S221-44995-91)

When installed on the autoinjector, this fan cools the vials in the rack. The fan turns ON and OFF along
with the autoinjector and can be moved along with the autoinjector.
2.6.2 Long Rack (P/N S221-45622-91)

The long rack is used for analyzing up to 12 sample vials (For details on the operation procedure etc., see
"2.3.2 Setting Vial Racks to the Long Rack Guide (Optional)" P.39).
Additionally, when using GC-14A/B or GC-2014, install this part and the mounting bracket for GC14/2014
(for AOC-20s) (S221-46217-92), which allows mounting the autosampler to the injection ports other than
the one at far left.
2.6.3 FPD Cooling Fan (P/N S221-44996-91)

This fan is a required accessory for the FPD detector and a GC-14, -15, and -16. The power is supplied
from the GC, so even when the autoinjector is turned OFF, the fan continues to operate as long as power is
supplied to the GC.
The cooling fan is installed on the autoinjector. Use care when removing the autoinjector with a cooling fan
attachment, because the fan is not fixed to the autoinjector.
The FPD cooling fan cable can be disconnected if necessary (for example, when moving the autoinjector
away from the GC). Be sure to turn the gas chromatograph power OFF before removing the cable.
Figure 2-50
2.6 Available Options
@ NOTE
When installing the autoinjector to the INJ located at the front of the FPD, use the FPD main unit cover
attached to the standard cooling fan.
2
Cover attached to the FPD cooling fan
Figure 2-51
If the autoinjector is installed to an INJ other than that located at the front of the FPD, use the standard FPD
cover (FPD main unit accessory). This prevents accidental contact with the FPD surface, which can cause
burns.
2.7 Installing the Autosampler (AOC-20s) [Optional]

2.7.1 Installing the Vial Racks
Six 1.5 ml vial racks (each holding up to 25 vials) are provided. Additionally, 4 ml vial racks are available as
optional parts. (See "2.7.3 Using 4 ml Vials (Option)" P.70.) Both rack sizes are installed in the same way.
1 The autosampler sample tray is divided into sections for positioning the vial racks. Seat
the vial racks between the sections, in numerical order, as shown in Figure 2-52.
An empty rack does not need to be placed in the autosampler for it to function.
Figure 2-52
2 The arm of the autosampler can be rotated manually when the power is ON (unless the
autosampler is operating). If necessary, the arm can be moved out of the way when
placing a rack on the autosampler.
Figure 2-53
Figure 2-54
! CAUTION
• Although the arm rotates manually, do not move it up and down or horizontally unless
you need to do so. Do not attempt to rotate the arm beyond the movement range.
When carrying the autosampler, do not hold it by its arm. Otherwise, it may be
damaged.
To identify vial positions, the labels shown below are available. See Figure 2-55 and Figure 2-56 for the vial
position numbers.
@ NOTE
2
When vial racks are not placed continuously, sample vial numbers are assigned sequentially skipping the
places with no vial rack. For details, see "3.4.1 Preparation", "(3) Placing vials in the autosampler tray" P.118.
Figure 2-55 1.5 ml vials
Figure 2-56 4 ml vials
Figure 2-57
2.7.2 Setting up Autoinjector Vial Racks

When using the autosampler, the standard autoinjector vial racks must be modified. The increased vial
capacity of the autosampler means that additional solvent and waste liquid vials are necessary. Vial racks
1 and 3, shown in Figure 2-58, are standard autoinjector accessories. Two additional solvent/waste liquid
vial racks 3 are provided with the autosampler. (Note that 2 4 ml vial rack is available as an option).
1 1.5 ml vial rack for 2 4 ml vial rack for autosampler 3 Solvent/waste liquid vial
autosampler × 1 (S221-45182) (Option) rack (4 ml) × 2
(S221-45181) (This holder is included in the 4 ml (S221-32949-01)
sample vial rack set (S221-41583-91).)
Figure 2-58
First, remove the current vial racks (for 6 vials) supplied with the autoinjector, by pulling them up. Then,
insert the new vial racks in the arrangement shown in Figure 2-59. Spread the rack guide apart slightly to
insert the vial racks. See Figure 2-60 and Figure 2-61 for details on vial placement.
3 from Figure 2-58

1 or 2 from Figure 2-58 3 Standard autoinjector vial rack
Figure 2-59
Arrange the 1.5 ml or 4 ml vials as shown in Figure 2-60 or Figure 2-61.
1 1.5 ml vial rack for autosampler 3 Solvent/waste liquid vial rack (4 ml)
(S221-45181) (S221-32949-01)
S = Solvent
W = Waste liquid
For sample (1.5 ml) For solvent flush injection or internal standard (4 ml)
Figure 2-60 1.5 ml vial arrangement
2 4 ml vial rack for autosampler (S221-45182) (option) 3 Solvent/waste liquid vial rack (4 ml)
(This holder is included in the 4 ml sample vial rack set (S221-32949-01)
(S221-41583-91).)
S = Solvent
W = Waste liquid
For sample (4 ml) For solvent flush injection or internal standard (4 ml)
Figure 2-61 4 ml vial arrangement
As indicated in Figure 2-60 and Figure 2-61, solvent A (S) is discharged into the waste liquid A vial (W),
and likewise for B and C. When washing with 3 solvents, each of the waste liquid is discharged into the
waste liquid vials marked A to C. When washing with only one solvent, the waste liquid of sample wash is
discharged into the waste liquid vial corresponding to the used wash solvent.
Both sizes of vial racks have pegs on the bottom. When inserting the racks, the pegs should appear at the
end of the rack guide, as shown in Figure 2-62.
Vial rack pegs
Figure 2-62
@ NOTE
Use the vial racks supplied with AOC-20s autosampler by mounting to AOC-20i rack guide. There are two
types of AOC rack guides of gray or black material. There is no problem in using vial racks with colors
different from that of a rack guide.
@ NOTE
To use the autosampler, complete the following settings.
• For GC-2010/2010Plus/2014/2025
2 Press the toggle key to display [Sampler] on the PF menu. 2
3 Press the [Sampler] (PF1) to display the [Sampler] window.
4 Set [Use of the Sampler] to [Use].
Figure 2-63
chromatograph.
• For Other Gas Chromatographs

Set the parameter as follows.
FUNCTION 40.........."001" (Use the sampler.)
A solvent or standard needs to be placed in the second vial position shown in Figure 2-60 and Figure 2-61
only in solvent flush injection mode.
The 4 ml vial for solvent flush injections is placed in the vial rack. The autosampler allows use of a different
vial for solvent flush and solvent rinse to minimize cross contamination problems.
2.7.3 Using 4 ml Vials (Option)

4 ml vials can be used instead of 1.5 ml vials. A 4 ml vial rack assembly (S221-44878-91) includes the
accessories described below.
Table 2-4 4 ml vial rack assembly (S221-44878-91)
Name Part No. Q'ty
1 4 ml vial autosampler rack S221-44710-91 6
2 4 ml vial rack for autoinjector S221-45182 ∗ 1
3 Large septum (for 4 ml) S221-34266-92 2

1 set 50 each, Total
4 4 ml vial S221-34267-92 2
100 pieces
5 Large cap (for 4 ml) S221-34268-92 2
∗ This holder is included in the 4 ml sample vial rack set (S221-41583-91).
When using 4 ml vials, both the vial racks for both the autosampler and autoinjector must be changed. For
the installation procedure, see "2.7.1 Installing the Vial Racks" P.64 and "2.7.2 Setting up Autoinjector Vial
Racks" P.67.
2.7.4 Precautions for Using the Autosampler

Please observe the following precautions when using the autosampler.
(1) Make sure that the caps of the vials being used are securely fastened.
If a cap is loose or is at an angle, the autosampler may not be able to grip the vial properly, resulting
in an error.
(2) If the autoinjector has been removed, in order to replace the septum or glass insert, make sure that
the autoinjector is reattached in the correct position.
If the autoinjector is at an angle, the autosampler may not be able to grip the vial properly, resulting
in an error.
(3) Set the position of the autosampler arm correctly (in [Rack position] for GC-2010/2010Plus/2014/
2025, and in FUNCTION 94 for other GC). If the arm position is not adjusted correctly to the cap of
the vial in the autoinjector rack, perform the following procedure to teach the autosampler the
correct position (teaching function).
2.7.5 Adjusting Sample Gripping Position (Teaching)
1 Turn the autoinjector on while pressing both the [Function] and [Monitor] keys. (For GC-
2010/2010Plus/2014/2025, set "AOC POWER" to "On" in the [AOC Parameters] window
on the GC, and press the [ENTER] key.)
2 After the initialization process has completed, place a vial (1.5 ml or 4 ml vial) on the left
side of the autoinjector turret.
3 Set the rack guide position for the autoinjector by the following procedure:
For GC-2010/2010Plus/2014/2025
3 Specify the rack guide position.
[0] : GC-2014/2025 (All INJ positions)
[1] : GC-2010/2010Plus Front
[2] : GC-2010/2010Plus Rear = Standard position
Per factory default, the autosampler has been programmed so that the arm moves to the rack guide
on the autoinjector mounted to the positions above (the far left INJ for GC-2014/2025). You can also
specify the desired arm destinations by using "teaching" procedures. 2
Figure 2-64

Enter the sample gripping position ("000" - "002") for the FUNCTION 94 value.
"000": GC-14A/B, 15A, and 16A (All INJ positions)
"001": GC-17A (GC-1700, GC-18A) Front = Standard position
"002": GC-17A (GC-1700, GC-18A) Rear
Per factory default, the autosampler has been programmed so that the arm moves to the rack guide on
the autoinjector mounted to the positions above (the far left INJ for GC-14A/B, 15A, and 16A). You can
also specify the desired arm destinations by using "teaching" procedures.
To enter "001", perform the following key operation:

2 Set "094" using the arrow keys.
3 Press the [ENTER] key.
4 Set "001" using the arrow keys.
! CAUTION
• When setting the rack guide position (FUNCTION 94), be sure to set the value for the
correct rack position beforehand, and perform the teaching procedure.
4 Allow the FUNCTION 78 value ("000") to appear on the autoinjector display.

[1] : Press the [FUNCTION] key on the autoinjector.
[2] : Set "078" using the arrow keys.
[3] : Press the [ENTER] key.
5 Place a vial on the turret. Gently move the autosampler arm so that it is positioned level
with the vial.
To position the arm in the horizontal plane, first extend the arm slightly beyond the amount required and
then align it to the correct position. (The teaching will not be performed properly when the arm is fully
extended.)
To position the arm in the vertical plane, use the [SAMPLE WASH], [SOLVENT WASH], and [NUMBER
OF INJECTIONS] keys to move the arm to the position shown in Figure 2-65. (Never move the arm up or
down while holding it near the end.)
@ NOTE
There is a slight delay between when the keys are pressed and when the arm moves.
[SAMPLE WASH] key: up
[SOLVENT WASH] key: stop
[NUMBER OF INJECTIONS] key: down
Arm
2 - 4 mm
Vial
Turret
Figure 2-65
6 After the position is aligned, adjust the function value to "001", then press the [Enter]
key.
The arm will grip the cap of the vial once, then return to the home position.
2.7.6 Mounting bracket for GC14/2014 (for AOC-20) (Optional)

Usually, when using the autosampler with GC-2014 or GC-14A/B, you can install the autoinjector only to
the injection port at the far left INJ position, even if using the standard mounting bracket that comes with
the autosampler. To make the autoinjector available at all INJ positions, use the mounting bracket for
GC14/2014 (for AOC-20). Change the positions of the vial racks for autosampler on the long rack guide
according to the selected INJ position.
Vial racks for autosampler

INJ INJ INJ INJ
Change rack positions for different INJ positions. 1 2 3 4
Vials for solvents/waste liquid
Vial destination ∗ W S W S W S
2
GC front side
for INJ4
Vial destination for INJ2 Position for solvent flush vial
Vial destination for INJ3 Vial destination for INJ1
Figure 2-66
To use this option with GC-2014, press the gas chromatograph [OPTION] key to display the [AOC
Parameters] window, and complete the following settings for each sub screen.
<Sampler sub screen>
Use of the Sampler: [Use]
<AOC Other Parameters sub screen>
Rack: [Long]
Rack Position: [0]
To use this option with GC-14A/B (GC-15A/16A), complete the following settings via the autoinjector.
FUNCTION 40........ "001" (Use sampler)
FUNCTION 93........ "001" (Long rack guide)
FUNCTION 94........ "000" (Rack guide position: 0)
@ NOTE
Be sure to perform the teaching process.
@ NOTE
DO NOT use the short rack guide when the autosampler is set for the long rack guide.
If you do, the vial racks will fall from the autoinjector gear, causing an error.
@ NOTE
When using in the solvent flush injection mode
Regardless of the INJ position, the solvent vial for solvent flush method should be set at the position marked
with (∗) in Figure 2-66. Therefore, when using this option in the solvent flush injection mode, it is required to
prepare another sampler vial rack for the solvent flush vial.
• 1.5 ml vial rack for autosampler P/N S221-45181
• 4 ml vial rack for autosampler P/N S221-45182
! CAUTION
• When using the solvent flush injection mode at INJ1
If the vial racks for autosampler are mounted as shown in Figure 2-3, the autosampler gripper
interferes with the solvent flush vial, making the vial transportation impossible.
To use the solvent flush injection mode at INJ1, perform the teaching procedure described below.
1 Mount the vial rack for autosampler to the "vial destination for INJ2" described in Figure 2-66.
2 In the [AOC Other Parameters] window, set [Rack Position] to [1]. Then, perform the teaching
procedure.
• When using the solvent flush mode at INJ1

Rack guide position: [1]
• In cases other than above

Rack guide position: [0]
2.8 Controlling via Workstation

2.8.1 Controlling AOC-20i,s via GCsolution/GCMSsolution
(on GC-2010/2010Plus/2014/2025, GCMS-QP2010)
You can control the autoinjector AOC-20i/Autosampler AOC-20s by operating the computer via the gas
chromatograph. When the parameters set on the GC and GCsolution/GCMSsolution are not identical,
those on GCsolution/GCMSsolution are applied to the autoinjector operation.
Operate the GC-2010/2010Plus/2014/2025 keypad for the parameters not available via GCsolution/
GCMSsolution. (Operate the autoinjector keypad only for the parameters not available even via gas
2
chromatograph.)
For details on the setting procedure, refer to the instruction manual for each software.
2.8.2 Controlling AOC-20i,s Using CBM-101/102

(on GCs Other than GC-2010/2010Plus/2014/2025)
The AOC-20 can be controlled by CLASS-VP software, version 4.3 or later, CLASS-GC10 software,
version 1.60 or later, and GCsolution, version 2.0 or later.
The AOC-20 series includes a fiber optic interface as standard equipment. Therefore, when controlling the
gas chromatographs via GCsolution or CLASS-GC10, only a fiber optic cable (2 m) is required (P/N: S070-
92025-52).
The fiber optic cable is connected to the 3-channel fiber optic CBM-101/102 interface equipped for GC
control.
An example system configuration for a GC-17A Ver.3 + AOC-20i (+ AOC-20s) + CLASS-GC10 is
described below.
Name Part No.

GC-17A Ver.3
AOC-20i (+AOC-20s)
CLASS-GC10DOS/V S223-04142-91
English version S223-04142-92
CBM-101 S223-04243-91
3-channel fiber optic interface S223-03727-91
Fiber optic cable (2 m) S070-92025-52
Operate the autoinjector keypad for the parameters not available via GCsolution or CLASS-GC10. For
details on the setting procedure, refer to the instruction manual for each software.
2.8.3 Installation for GCMS-QP5000

AOC control for GCMS-QP5000 is performed by CLASS-5000 software. Currently, CLASS-5000 software
does not support all AOC functions, and should be operated in AOC-20 (AOC-17) mode.
The following restrictions apply when using AOC-17 mode:
(1) The valid ranges for conventional parameters supported by CLASS-5000 are the same as those for
AOC-17.
Conventional parameters AOC-17 mode AOC-20 mode

Number of sample washes 0 - 20 0 - 99
Number of solvent washes 0 - 20 0 - 99
Wait time after aspiration 0 - 9.9 sec 0 - 99.9 sec
Plunger injection speed low/high low/mid/high
Syringe injection speed low/high low/high
Wait time after injection 0.3 - 9.9 sec 0 - 99.9 sec
Solvent flush 0, 1, 2, 3 0, 1, 2, 3, 4 Settings 3 and 4 are used with
Autosampler.
Number of pumpings 0 - 20 0 - 99
Solvent selection 0, 1, 2, 3 0, 1, 2, 3
Sample injection volume 0.1 - 8.0 μl 0.1 - 8.0 μl
Number of sample injections 1 - 100 1 - 10
Number of samples when 1 - 100 1 - 150 Note 1)
autosampler is used
Note 1) For 4 ml vials, the number of samples is 96.
(2) The CLASS-5000 software does not support new parameters introduced with AOC-20, such as
Aspiration to needle tip, Needle lower position, etc. However, these functions can be controlled
using the AOC-20i keys.
When connected to the GCMS-QP5000, a 2-m fiber optic cable (P/N S070-92025-52) is required, similar to
that for CLASS-GC10 software. A conventional AOC-17 Link Kit is not required.
2.8.4 Chromatopac C-R7A Series GC Network Control

The following Rel. No. can be used to control an AOC-20 over a GC network.
• C-R7A (e)
C-R7A system disk R3.61 or later (System V3.6 or later)
Utility disk R3.61 or later
• C-R7A (e) plus

C-R7A system disk R5.51 or later (System V5.5 or later)
Utility disk R3.61 or later
2
A three channel optical interface (P/N S223-03727-91) is required on the C-R7A side.
A 2-m fiber optic cable (P/N S070-92025-52) is required to connect the C-R7A and AOC.
3 3 Operation
3.1 Basic Operation 3

3.1.1 Loading Vials in the Rack
Place vials in the rack as shown in Figure 3-1. (See "2.4.3 Vial Placement" P.55.)
3
@ NOTE
The vial rack can be removed when the autoinjector is in home position. When re-installing the rack, insert it
3
into the rack guide until the gears catch, then at least 20 mm more, and press the [RESET] key. (See "2.3.3
Loading Rack into the Autoinjector" P.41.)
To insert the rack during analysis, press the [STOP] key instead of the [RESET] key. (See P.44.)
3
3
Waste liquid Solvent vial

3
Vials (1.5 ml) vial (4 ml) (4 ml)
Figure 3-1 3
Vial positions are designated by numbers 1 - 6 (1 - 12 for the optional long rack), as shown in the figure
above. A single vial can be placed in any of these positions; it will be automatically detected and injected. If
there are two or more vials in the rack, injections occur from the lowest numbered position to the highest.
3
For example, if vials occupy all 6 positions, injections will occur, in order, from position 1 to position 6.
3
3
3
3
3
3
3
3
3 Operation
3.1.2 Turning the Power ON/OFF

Turn ON the GC power when using GC-2010/2010Plus/2014/2025 (AOC built-in power type). Turn ON the
autoinjector power when using the other gas chromatographs (AOC external power type).
After tuning ON the power, the autoinjector automatically performs the following operations, and moves to
its home position:
• Plunger alignment
• Syringe alignment
• Moving the rack guide, plunger, and syringe to their home positions
 Autoinjector Home Position

The home position refers to the autoinjector status in which the syringe, plunger, and rack are in their home
positions.
Syringe: Highest position
Plunger: Lowest point
Rack: Far left position
The autoinjector stops its operation when the [STOP] key is pressed, or while the autoinjector is waiting for
the GC READY status. However, this does not necessarily mean the autoinjector is at its home position.
The autoinjector is at its home position after the power is turned ON or the initialization process is
completed. Otherwise, pressing the [RESET] key will set the autoinjector to its home position.
For GC-2010/2010Plus/2014/2025
@ NOTE
When using GC-2010/2010Plus/2014/2025 (AOC built-in power type), when the autoinjector does not
operate even if turning ON the gas chromatograph:
• Verify that the AOC cable is connected properly.
• Press the [OPTION] key to display the [AOC Parameters] window, and verify that [AOC POWER] is set to
[On].
@ NOTE
When the [AOC Parameters] window is not displayed even if pressing the [OPTION] key many times,
perform the following procedure:
1 Press the [SET] key on the gas chromatograph to display the [Analysis Settings] window.
2 Select [LineConfg] (PF2 key) to display the [Line Configuration] window.
3 Specify the AOC for the analytical line.
@ NOTE
If the message "AOC link ok." is not displayed, check if the RS-232C cable is properly connected to the AOC
power supply unit terminal on the GC back panel.
@ NOTE
When using gas chromatographs other than GC-2010/2010Plus/2014/2025 (AOC built-in power type), turn
OFF the autoinjector power supply unit first, then turn OFF the gas chromatograph. If the GC is turned OFF
while the autoinjector is waiting for analysis, the autoinjector may start an injection. If it is necessary to turn
OFF the gas chromatograph before the autoinjector, verify beforehand that the autoinjector is in the home
position.
3.1 Basic Operation
3.1.3 AOC-20i Keypad
Display
Arrow keys
Parameter keys
3
MONITOR key
FUNCTION key
ENTER key
RESET key
RUN light
START key / STOP key
Figure 3-2
! CAUTION
• GC-2010/2010Plus/2014/2025 [AOC Parameters] and AOC-20i Keypad
For GC-2010/2010Plus/2014/2025, operate the gas chromatograph when changing the AOC
parameters accessible from the gas chromatograph. When you have set those parameters using the
autoinjector keypad, perform the following procedure to change the AOC parameters set on the GC
according to your change:
1 Press the [OPTION] key to display the [AOC Parameters] window.

2 Press [Upload] (PF menu) to upload the autoinjector parameters to the gas chromatograph.
The parameters not accessible from GC-2010/2010Plus/2014/2025 are indicated with "∗" in
"Corresponding AOC Parameters/Other Parameters on GC" in the table shown in "3.2.8 FUNCTION
Key" P.92.
3 Operation
• Display
All set values, sample numbers and error codes (numerical values displayed at an error occurrence)
are displayed here. All parameter settings are either 000 or positive numbers. Error codes are negative
numbers or numbers that begin with "E". When an invalid value is entered, "- - -" is displayed.
@ NOTE
When using GC-2010/2010Plus/2014/2025, error messages also appear on the gas chromatograph display.
• Arrow keys
These three keys change the values displayed. Each key corresponds to the right, center and left digit,
respectively. Values changed with these keys do not become valid until the [ENTER] key is pressed.
• Parameter keys
These display and set the various parameters. When one of these keys is pressed, the LED to its left
illuminates.
@ NOTE
To set the parameters corresponding to these keys on GC-2010/2010Plus/2014/2025, be sure to operate via
the gas chromatograph.
• MONITOR key
During analysis, the [MONITOR] key is automatically activated, so that the current sample number is
displayed. To display a parameter's current value, press the [MONITOR] key, then press the parameter
key (within one second).
• FUNCTION key
This key is used to set parameters (except for those parameters which have a parameter key, such as
SAMPLE WASH), and for customizing or specifying optional accessories.
When the [FUNCTION] key is pressed, a function number beginning with "F" is displayed (this is the
most recently accessed function number). To view a different function, use the arrow keys to change
the function number. Then press [ENTER] to display the current function value. To change the current
value, use the arrow keys, then press [ENTER] to save the setting.
@ NOTE
When using GC-2010/2010Plus/2014/2025, use these keys only for setting the parameters not accessible
via the gas chromatograph.
• ENTER key
This saves the displayed numerical value as the current setting. When the [ENTER] key is pressed, the
LED to its left illuminates momentarily.
• RESET key
This key returns the autoinjector to the home position. All the parameter values remain in battery-
powered reserve memory even if the [RESET] key is pressed.
@ NOTE
When using GC-2010/2010Plus/2014/2025, the same function can be accessed through [Reset] (PF menu)
in the gas chromatograph [AOC Parameters] window.
• Run light
This light is either on or blinking, according to the autoinjector status.
Light on .................indicates that the autoinjector is performing a task.
Light blinking .........indicates that the autoinjector is waiting for completion of the gas chromatograph
analysis (waiting for the GC READY status).
3.1 Basic Operation
• START key
This initiates the injection process.
@ NOTE
When using GC-2010/2010Plus/2014/2025, the same function can be accessed through [Start] (PF menu) in
the gas chromatograph [AOC Parameters] window.
• STOP key
Pressing the [STOP] key causes the autoinjector to stop after completing the current operation. The
process resumes where it left off when the [START] key is pressed.
@ NOTE
When using GC-2010/2010Plus/2014/2025, the same function can be accessed through [Stop] (PF menu) in
the gas chromatograph [AOC Parameters] window.
3
3.1.4 Setting Parameters
The parameter default values have been preset to the autoinjector at the factory. Additionally, those
autoinjector parameter default values have also been preset to the gas chromatograph GC-2010/
2010Plus/2014/2025 at the factory. You can change these parameters as your analytical purposes dictate.
@ NOTE
When changing the parameters accessible from the gas chromatograph GC-2010/2010Plus/2014/2025, be
sure to operate from the GC.
The parameters not accessible from GC-2010/2010Plus/2014/2025 are indicated with "∗" in "Corresponding
AOC Parameters/Other Parameters on GC" in the table shown in "3.2.8 FUNCTION Key" P.92.
To set autoinjector parameters when using GC-2010/2010Plus/2014/2025, turn ON the gas chromatograph
to link it to the autoinjector. The parameter values in the file loaded on the GC are automatically transmitted
and set to the autoinjector. You can also change parameters in the GC [AOC Parameters] window
(including sub screens). After changing the parameters, press the [ENTER] key on the GC. The changed
parameters will be transmitted to the autoinjector.
Note that even though you can change the parameters by pressing the [ENTER] key on the autoinjector,
the AOC parameters set on the gas chromatograph remain unchanged.
This means that the next time the GC is linked to the autoinjector (e.g. when the GC is restarted, etc.), the
autoinjector parameters (accessible via the GC) are overwritten by all the AOC parameters transmitted
from the GC.
Example: Changing SAMPLE WASH from 2 times to 3 times
For GC-2010/2010Plus/2014/2025
1 Press the [OPTION] key to display the [AOC Parameters] window.
2 Use the [ ] or [ ] keys to move the cursor to [Sample Wash].
3 Operation
3 Enter [3] from the gas chromatograph keypad and press the [ENTER] key.
@ NOTE
DO NOT change the parameter by pressing the [SAMPLE WASH] key on the autoinjector.
Figure 3-3
@ NOTE
When setting parameters, the [ENTER] key must be pressed for the new parameter to take effect.
Otherwise, the old parameter value will be used. If an invalid value is entered, the autoinjector does
not process it. "Input parameter out of range" is displayed, and the previously set value remains in
effect.
1 Press the [SAMPLE WASH] key.

The LED to the left of the key illuminates and the display shows the current setting. ("002", as the initial
value)
2 Press the [Right arrow] key once.

The value on the display increases to "003".

SAMPLE WASH is now set for 3 washes. (The [ENTER] key LED blinks once.)
3.1 Basic Operation
Example: Changing solvent aspiration volume for solvent flush mode

Even for GC-2010/2010Plus/2014/2025, you cannot set this parameter via the gas chromatograph.
Press the autoinjector [FUNCTION] key to change the F35 parameter.
@ NOTE
When setting parameters, the [ENTER] key must be pressed for the new parameter to take effect.
Otherwise, the old parameter value will be used. If an invalid value is entered, the autoinjector does not
process it. "- - -" is displayed, and the previously set value remains in effect.
@ NOTE
Pressing any of the parameter keys causes its current value to be displayed. However, the SAMPLE
NUMBER parameter displays automatically without pressing the key. (See "3.1.6 Operation Precautions",
"Vial Detection" P.88.) Therefore, if an injection sequence is started while setting a parameter, the SAMPLE
NUMBER may be displayed. In this case, reset the parameter.
3
3.1.5 Basic Injection Procedure
The parameters required for sample injection are already set as default values in the autoinjector. (See
"3.2.2 Parameter Keys" P.89 and "3.2.8 FUNCTION Key" P.92.) Therefore you can easily perform sample
injections by (1) setting vials on the rack guide, (2) turning ON the power, and (3) pressing the [START] key
(alternatively for GC-2010/2010Plus/2014/2025, [Start] (PF menu) in [AOC Parameters] on GC.)
Create the desired injection conditions by changing the following 4 parameters, etc.: SAMPLE WASH
(Default: 2 times), SOLVENT WASH (Once), NUMBER OF INJECTIONS (Once), and SAMPLE SIZE
(1.0 μl). (See "3.2.2 Parameter Keys" P.89.)
The changed parameters are saved to the autoinjector even after the power is turned OFF.
Table 3-1
GC-2010/2010Plus/
Key Name 2014/2025 Description Setting Range Default Value
Item Name
SAMPLE WASH Sample Wash Sample washing times of the 0 - 99 times 2 times
syringe before injection
SOLVENT WASH Solvent Wash Solvent washing times of the 0 - 99 times Once
syringe after injection
NUMBER OF Number of Injection Injection times for the same 1 - 99 times Once
INJECTIONS samples
SAMPLE SIZE Sample Size (μl) Sample injection volume 0.1 - 8.0 μl 1.0 μl
If the [START] key is pressed on the autoinjector (For GC-2010/2010Plus/2014/2025, [Start] (PF menu) in
[AOC Parameters]), the injection is performed by the following procedure:
(1) Start
If the GC is READY when the [START] key is pressed, the autoinjector immediately starts injection
sequences. If not, the autoinjector starts the sequences after the GC becomes READY. However, an
injection cannot be started if an error code (-01 to -04: see "6.1 Error Codes" P.151) is displayed. In
such a case, identify and remove the cause of the error, press the [RESET] key to return the AOC to
its home position (the indication returns to "000".), and press the [START] key again.
If the GC is not READY when the [START] key is pressed, the autoinjector stands by until the GC
becomes READY, blinking the RUN light. If the [START] key is pressed again during the stand-by,
the injection process begins, regardless of the GC status.
3 Operation
(2) Wash before injection

When "PRE SOLVENT WASH" [FUNCTION 01] is set, the syringe is washed with solvent. The initial
setting is [0] (no solvent wash before injections).
During a sample wash, the syringe aspirates the sample then discards it. Using sample washes
increases injection reproducibility. Specify the washing times by the parameter [Sample Wash]. For
example, if the value is set to [2], two sample washes will be performed before injection.
@ NOTE
When pumping and washing the syringe with sample or solvent, the plunger rises to aspirate the
designated amount (8 μl using 10 μl syringe in the default setting), then holds that position for a
certain time. The plunger is then pushed down to expel the contents into the waste vial. When
aspirating high viscosity samples, increase the plunger hold time, since it takes some time for the
sample to enter the syringe after the plunger is raised.
For GC-2010/2010Plus/2014/2025
Set the waiting time during sample wash with the parameter of [Viscosity(s)]. For example, if the set
value is [0.2], the wait time is 0.2 seconds.

Set the wait time with FUNCTION 03 (VISCOSITY). For example, if the set value is "00.2", the wait
time is 0.2 seconds.
(3) Pumping
If bubbles are included in aspirated samples, the reproducibility of the sample aspiration quantity is
deteriorated. The autoinjector performs "pumpings" to pump out the bubbles from the samples.
The default value is 5 times. The number of pumpings can be changed.
For GC-2010/2010Plus/2014/2025: Pumping
For other gas chromatographs: FUNCTION 02
After the pumping is complete, the autoinjector aspirates samples with the specified volume. For
example, if the value is set to [1.0] ("01.0") when using 10 μl syringe, the autoinjector aspirates 1 μl
of the sample.
@ NOTE
The set value of [Viscosity(s)] or VISCOSITY also applies for the wait time during pumping. For
example, if the value is set to [0.2] ("00.2"), the wait time is 0.2 seconds. After aspirating the sample
for the actual injection, however, the autoinjector always waits for 4 seconds, even if the value is set
to 4 seconds or smaller. If the value is set to 4 seconds or longer, the autoinjector waits as long as
the set value.
(4) Sample injection

After the pumping is complete, the autoinjector aspirates the sample and injects it to the GC
injection port. First the syringe is lifted down and the needle is inserted into the INJ port. Then the
plunger is pressed down and the sample is injected.
For GC-2010/2010Plus/2014/2025
Select the plunger speed at injections from [Slow], [Middle], and [Fast] in [Inj. Speed(Plunger)] in the
[AOC Parameters] window. Select the syringe up/down speed from [Slow] and [Fast] in [Inj.
Speed(Syringe)] in the [AOC Other Parameters] window.

Select the plunger speed at injections from Low ("000"), Medium ("001"), and High ("002") with
FUNCTION 05. Select the syringe up/down speed from Low ("000") and High ("001") with
FUNCTION 06.
3.1 Basic Operation
(5) Wash after injection

The solvent wash rinses the syringe following injection to minimize contamination problems.
For GC-2010/2010Plus/2014/2025
The wash times can be set in [Solvent Wash] in the [AOC Parameters] window. For example, if the
value is set to [1], one solvent wash is performed.

Set the number of washes with the [SOLVENT WASH] key. For example, if the value is set to "001",
one solvent wash is performed.
@ NOTE
3
The wait time for solvent wash is fixed to 0.2 seconds regardless of the [Viscosity(s)] and
[VISCOSITY] settings.
(6) Return to home position

After the sequence above is complete, the syringe, plunger, and rack return to their home positions.
The autoinjector then waits for the gas chromatograph to complete the analysis (the autoinjector
RUN lamp blinks, and the gas chromatograph [AOC Parameters] displays [Wait]). When the
analysis is complete and the GC becomes READY, the autoinjector begins the next injection
process.
If 2 or more vials are set in the rack, the process described above is performed on each of the
samples.
If a value higher than [1] is set to [Number of Injection] in [AOC Parameters] for GC-2010/2010Plus/
2014/2025, or "NUMBER OF INJECTIONS" for other gas chromatographs, the process is repeated
on each sample according to the value.
3 Operation
3.1.6 Operation Precautions
 Parameter Settings
When an invalid parameter value is entered, the previous set value remains unchanged. (When using GC-
2010/2010Plus/2014/2025, "Input parameter out of range" appears in the GC display, and when the value
is entered with the autoinjector keypad, "- - -" is displayed.)
If a parameter is changed after an injection sequence has begun, the previous value is applied to the
current sequence. For example, if a SAMPLE WASH value is changed from 2 to 3 during a sample wash,
only two sample washes will be performed. The new setting will take effect upon the next injection, when
sample wash will be performed three times.
 Reset and Stop

The "STOP" refers to pausing an injection process temporarily; the "RESET" refers to stopping the
injection entirely.
If an injection process is stopped while the autoinjector is actively functioning, the injection process pauses
after completing the current operation. If the injection process is started, it resumes from the sequence next
to the interrupted sequence.
If an injection process is reset while the autoinjector is actively functioning, the autoinjector promptly
returns to home position. (However, if the autoinjector is in operation, it is better to first stop the procedure
before resetting it.)
If the injection process is restarted (after it is reset), it will start from the beginning. If an error ("-01" to "-04")
is displayed, reset the injection process to return the injector to the home position, clearing the error.
Reset the injection process to return the autoinjector to the home position in the following situations:
• To re-start the injection process after the process has started.
• To terminate analyses during an analysis sequence (see "3.3 Sequential Analyses and Injection Modes"
P.104).
• To clear an error code ("-01" to "-04": see "6.1 Error Codes" P.151) which appeared when attempting to start
an injection process.
Stop the injection process to pause the autoinjector temporarily in the following situation:
• To temporarily pause the injection process (for confirming the presence of sample in the syringe or to change
parameter settings, for example).
 Vial Detection
The autoinjector uses a photo-sensor to automatically detect vials in the rack. If there are no sample vials,
the injection process is not performed; if there are no solvent vials, the solvent wash procedure is not
performed.
A vial can be placed in any position of the rack to start injection. When vials are placed in several positions,
injections are made in ascending order from the lowest position number.
@ NOTE
If the autoinjector detects no waste vial, an error occurs and the autoinjector stops operating as the contents
of the syringe cannot be expelled. Always place a waste liquid vial in the rack.
@ NOTE
To allow error-free vial detection, use only white vials and caps. Avoid allowing strong light to shine directly
on the vial detection sensor, as this can cause detection errors.
3.2 Display and Keypad Functions
! CAUTION
• For GC-2010/2010Plus/2014/2025, operate the following keys only for setting the
parameters not accessible via the gas chromatograph.
The parameters not accessible via GC-2010/2010Plus/2014/2025 are indicated with "∗" in
"Corresponding AOC Parameters/Other Parameters on GC" in the table shown in "3.2.8 FUNCTION
Key" P.92.
3.2.1 Display and Arrow Keys 3

• The display shows parameter values and errors.
When an invalid parameter value is entered, "- - -" is displayed and the LED to the left of the [ENTER] key
remains on.
• Use the arrow keys to increase the displayed value when setting parameters. The displayed value cannot be
lowered; to enter a lower number, cycle through to the highest value and start again.
Each digit is increased by the corresponding arrow key below it. This means that as the right digit increases
(even past 9), the center digit is not affected.
3.2.2 Parameter Keys

(1) SAMPLE WASH
Sets the number of times the syringe is rinsed with sample before sample injection.
Default Setting: Twice ("002")
Valid Range: 0 - 99 times ("000" - "099")
Change set values as required, considering the carry-over of the previous sample or solvent wash.
(2) SOLVENT WASH

Sets the number of times the syringe is rinsed with solvent after the sample is injected.
Default Setting: Once ("001")
Change set values as required, considering the carry-over of the previous sample or solvent wash.
(3) NUMBER OF INJECTIONS

Sets the number of times to inject each sample. If this number is changed during an injection
process, the new value will be used for the remaining samples in the sequence.
Default Setting: Once ("001")
This value applies for all samples.
(4) SAMPLE SIZE

Sets the volume of sample to be injected, in μl [Set value (%) × Syringe volume].
Default Setting: 1 μl ("01.0") [when using 10 μl syringe]
Valid Range: 0.1 μl - 8.0 μl ("01.0" - "08.0") [when using 10 μl syringe]
3 Operation
@ NOTE
Be sure to set these parameters on the gas chromatograph. Otherwise, the autoinjector parameters between
the autoinjector and GC become inconsistent.
Table 3-2
Corresponding AOC Setting Default

Key Name Description
Parameters on GC Range Value
Sample Wash SAMPLE WASH Sample washing times of the 0 - 99 times 2
syringe before injection
Solvent Wash SOLVENT WASH Solvent washing times of the 0 - 99 times 1
syringe after injection
Number of Injection NUMBER OF Injection times of identical samples 1 - 99 times 1
INJECTIONS
Sample Size SAMPLE SIZE Sample injection volume ∗ ∗
∗ Syringe Volume Setting Range Default Value (μl)

0.5 0.01 - 0.50 0.10
5 0.1 - 4.0 0.5
10 0.1 - 8.0 1.0
50 0.5 - 40.0 5
250 5 - 200 25
3.2.3 MONITOR Key

• The [MONITOR] key automatically displays the number of the sample currently being analyzed. This key is
not used for setting values.
The number of the sample being analyzed is automatically entered by vial detection.
• Default value: 0 ("000")
• Possible display range: 1 - 6 ("001" - "006")......................... With short rack
1 - 12 ("001" - "012")....................... With long rack
1 - 150 ("001" - "150")..................... With autosampler
When no vial is detected, "000" is displayed. When the autoinjector starts, the rack moves and the vial is
detected. When a vial is detected, its position number (the smallest value if multiple samples are placed) is
read and displayed.
• The current value of a parameter can also be monitored. Parameters that can be monitored are:
- SAMPLE WASH, SOLVENT WASH, NUMBER OF INJECTIONS (analysis count), analysis time, analysis
start time, and pumping.
- To display the current number value of the first three parameters, press the [MONITOR] key, then
immediately press the corresponding parameter key (within one second).
- Both the [MONITOR] and the parameter key LEDs will illuminate.
- To see the analysis start time (FUNCTION 32) and the analysis time (FUNCTION 31), press the
[MONITOR] key, then the [FUNCTION] key.
- To see the number of pumpings, press the [MONITOR] key, then the [SAMPLE SIZE] key.
3.2.4 ENTER Key

• This key saves parameters values. When this key is pressed, the LED to its left lights momentarily, and the
displayed value is saved.
When a displayed value is changed, it will not be saved until the [ENTER] key is pressed.
• If an invalid parameter value is entered, it cannot be saved even if the [ENTER] key is pressed. The display
shows "- - -", and the LED at the left of the [ENTER] key remains on.
3.2.5 RESET Key

• This key forcedly aborts the injection process and returns the autoinjector to the home position. The
[RESET] key also clears errors ("-01" to "-04"), and returns the autoinjector to the home position.
@ NOTE
That if the [RESET] key is pressed during a sequence of analyses, all information on the number of
completed analyses and injections is lost.
3
• For GC-2010/2010Plus/2014/2025, [Reset] (PF menu) in the [AOC Parameters] window on GC has the
same function.
3.2.6 START Key

• This key initiates the injection procedure.
If, for some reason, the syringe, plunger or rack did not return to home position, causing an error, the
autoinjector cannot be started. (To clear the error, press the [RESET] key, then the [START] key.)
• If the [STOP] key was pressed to pause the injection process, press the [START] key to resume the injection
process from the sequence next to the interrupted sequence.
• Press the [START] key after the [RESET] key to begin the injection procedure from the first sample.
• Once the [START] key has been pressed, the autoinjector waits until the gas chromatograph becomes
READY. During this wait time, the RUN light blinks.
The autoinjector can be forced to start injection before the GC is READY; press the [START] key again, and
the injection process starts immediately.
• For GC-2010/2010Plus/2014/2025, [Start] (PF menu) in the [AOC Parameters] window on GC has the same
function.
3.2.7 STOP Key

• This key pauses the injection process. Unlike the [RESET] key, the [STOP] key does not return the
autoinjector to the home position. Therefore, pressing [START] after [STOP] resumes the injection process
from the sequence next to the interrupted sequence.
• When the [STOP] key is pressed, the current action is completed before the autoinjector stops operation. For
example, if the [STOP] key is pressed while the plunger is rising, the plunger will not stop midway, but will
continue until it has reached its highest point.
• For GC-2010/2010Plus/2014/2025, [Stop] (PF menu) in the [AOC Parameters] window on GC has the same
function.
3 Operation
3.2.8 FUNCTION Key

• The [FUNCTION] key is used to change and check the status of all the functions. When the [FUNCTION] key
is pressed, the most recently used function number is displayed with an "F", and the function key LED
illuminates. Change the numerical value to the desired FUNCTION number and press the [ENTER] key. The
current value for the function is displayed. Change the numerical value and press the [ENTER] key to
change the value.
Example: FUNCTION 03 (Viscosity): Changes [Viscosity(s)]

Table 3-3
Key operation Display

1 FUNCTION F02 Displays the last FUNCTION No. (In this example,
FUNCTION 02 was the last function used.)
2 Right arrow key F03 Press the arrow key once; the display becomes "F03".
3 ENTER 00.2 The current value of FUNCTION 03 is displayed. (In this
example, the hold time is set to 0.2 sec.)
4 Right arrow key 00.5 Press the arrow key three times, and the display becomes
"00.5".
5 ENTER 00.5 Sets the plunger hold time to 0.5 sec.
• The function setting will display, and the FUNCTION LED stays on, until a key other than an arrow key or
[ENTER] is pressed.
• For GC-2010/2010Plus/2014/2025, use the autoinjector keypad only when setting the FUNCTIONs not
accessible via the GC. (i.e., FUNCTIONs indicated with "∗" in the following table, "Corresponding AOC
Parameters/Other Parameters on GC")
(1) FUNCTION 01 - 08
The FUNCTION 01 - 08 are the parameters for injection sequence.
Table 3-4

FUNCTION Name Description
Parameter on GC Range Value
Pre Solvent Wash F01 PRE SOLVENT Solvent washing times 0 - 99 times 0
WASH of the syringe before
injection (Note 1)
Pumping F02 PUMPING Pumping times of the 0 - 99 times 5
syringe
Viscosity F03 VISCOSITY Plunger waiting time 0 - 99.9 0.2
after sample aspiration
(Note 2)
sec.
3
Dwell Time F04 DEWELL TIME Waiting time after 0 - 99.9 0
sample injection sec.
Inj. Speed (Plunger) F05 INJ SPEED- Injection speed of the 0: Low 2
PLUNGER plunger 1: Medium
2: High
Corresponding AOC
Setting Default
Other Parameter on FUNCTION Name Description
Range Value
GC
Inj. Speed (Syringe) F06 INJ SPEED- Injection speed of the 0: Low 1
SYRINGE syringe 1: High
Inj Mode F07 INJECTION MODE Injection mode 0 - 4 (Note 3) 0
Solvent Select F08 SOLVENT SELECT Solvent selection (Valid 0 - 3 (Note 4) 0

only when using the
autosampler, or
F28 = 1)
Note 1) FUNCTION 01 is available only in Normal injection mode (when FUNCTION 07 is set to [0]).
Note 2) Wait times vary, depending on the injector operation. The wait time for both sample wash and pumping is
the FUNCTION 03 set time. When aspirating a sample for injection, the plunger holds either the set time,
or 4 sec, whichever is longer. The wait time for solvent washing is fixed at 0.2 sec.
Note 3) 0: sample + (air) --- Normal injection mode
1: Solvent + air + sample + (air) --- standard solvent flush mode (See Figure 3-14.)
2: Solvent + sample + (air)
3: Solvent + air + standard (internal standard) + air + sample + (air)
4: Solvent + standard (internal standard) + sample + (air)
Settings 3 and 4 are available only when an autosampler is connected.
The option of drawing air last is set with FUNCTION 20.
Note 4) 0: 3 vials of solvent are used.
1: Only solvent A is used.
2: Only solvent B is used. (See Figure 3-18.)
3: Only solvent C is used.
FUNCTION 08 is available only when the autosampler is connected and when FUNCTION 28 is set to [1].
3 Operation
These functions designate priority samples.
For details, see "3.3.2 Priority Analysis" P.111 when not using the autosampler, or "3.4.5 Priority
Analysis" P.123 when using the autosampler.
@ NOTE
For GC-2010/2010Plus/2014/2025, the [Priority] PF menu is displayed in the gas chromatograph [AOC
Parameters] window, only when the autoinjector is operating.
Table 3-5

FUNCTION Name Description
Parameter on GC Range Value
Priority F10 PRIORITY SAMPLE Priority sample No. No. 1 - 6 0
No. 1 - 12
No. 1 - 150
(Note 1)
(Note 2) F11 INJECTION Number of priority No. 1 - 6 0

SAMPLE injections No. 1 - 12
No. 1 - 150
(Note 1)
(Note 2) F12 FINAL SAMPLE Final priority sample No. No. 1 - 6 0

No. 1 - 12
No. 1 - 150
(Note 1)
(Note 2) F13 INJECTION Number of priority No. 1 - 6 0

SAMPLE2 injections No. 1 - 12
(for Sub-AOC) No. 1 - 150
(Note 1)
Note 1) Without autosampler, short rack ............ 1 - 6

long rack ............. 1 - 12
With autosampler................................... 1 - 150
When an autosampler is used, the valid range varies, depending on the vial size and racks installed.
The default value, "0", indicates that there is no priority sample.
Note 2) F11, F12, and F13 do not have corresponding AOC parameters for GC-2010/2010Plus/2014/2025 on the
gas chromatograph.
Use the gas chromatograph batch schedule function, which allows a similar operation.
These functions customize the instrument.
Table 3-6
Corresponding AOC Default

FUNCTION Description Setting Range
Other Parameter on GC Value
Air Suction F20 Specifies whether to aspirate air to the 0: No 0
syringe (Note 1) 1: Yes
F21 F21 Specifies wash solvent before injections in 0, 1 (, 2) 0

solvent flush mode (Note 2)
Plunger Suction Speed F22 Specifies the plunger speed at sample 0: Low 2
aspiration (Note 3) 1: Medium
Speed of Plunger F23 Specifies the speed of pressing down the

2: High
0: Low 2
3
plunger during sample wash and pumping 1: Medium
2: High
Suction Volume for F24 Specifies the aspiration amount for sample 0: 80% 0
Washing wash and pumping 1: 60%
of the F34 set
value
Syringe Height (↑) F25 Specifies the syringe height in vials (upper 0 - 20 mm 0
direction).
Syringe Height (↓) F26 Specifies the syringe height in vials (lower 0 - 2 mm 0
direction). (1.5 ml vial)
0 - 10 mm
(4 ml vial)
Syringe Height (Inj) F27 Specifies the syringe height at injections 0 - 22 mm 0
(upper direction).
Using 3 Solvent Vials F28 Specifies whether to use 3 solvents in the 0: 1 solvent 0
system without the autosampler. 1: 3 solvents
Kinds of Vials F29 Selects the vial size. 0: 1.5 ml 0
1: 4 ml
∗ F30 Specifies whether to check the position 0: No 0
when returning a vial to the autosampler. 1: Yes
∗ F31 Specifies the analysis time (time before the 0 - 655 min. 0
next injection).
∗ F32 Specifies the analysis start time (delay to 0 - 99.9 hour 0
start operation after the [START] key is
pressed).
Multi-Inj. F33 Specifies the number of injections per 1 - 99 1
analysis.
Kinds of the Syringe F34 Specifies the syringe size. 0: 10 μl 0
1: 50 μl
2: 250 μl
3: 0.5 μl
4: 5 μl
∗ F35 Specifies the solvent aspiration volume for 0: 10% 0
solvent flush mode. (Note 4) 1: 5%
of the F34 set
value
3 Operation
Note 1) Specify whether to aspirate 1 μl of air after sample has been drawn. This helps to avoid discrimination for
sample components with a wide boiling point range.
! CAUTION
• When using a high initial column temperature or a column with high polarity (such as
polyethylene glycol (PEG) phase), injecting air may shorten the column service life.
Note 2) The parameter settings for solvent wash before sample injection in SOLVENT FLUSH mode (when
FUNCTION 07 is set to [1] - [4].)
0: Number of times set by the SOLVENT WASH key (the same number before and after injection)
1: Number of times set by the SAMPLE WASH parameter
2: Number of times specified in F01 (PRE SOLVENT WASH). (This is invalid for GC-2010/2010Plus/2014/
2025.)
If the solvent flush mode is set for GC-2010/2010Plus/2014/2025, the GC [AOC Parameters] window does
not display [Pre Solvent Wash], and the solvent wash is performed the same number of times before and
after injections. If the F21 is set to [1], however, [Pre Solvent Wash] is displayed in the solvent flush mode,
which allows specifying different sample wash times before and after injections.
Note 3) The aspiration speed for sample wash and sample aspiration is variable. The aspiration speed for pumping
and solvent wash is fixed at HIGH speed.
Note 4) [5%] can be selected when Injection mode (FUNCTION 07) is set to [3] or [4]. Otherwise, a value of [10%]
is applied regardless of the setting.
These functions specify whether or not to use optional units.
Table 3-7

Use of the Sampler F40 Autosampler 0: Not Use, 1: Use 0
Use of the Sub AOC F41 Sub-injector 0: Not Use, 1: Use 0
Bar Code Reader F42 Bar code reader 0: Not Use, 1: Use 0
These functions set the dual injector parameters. For details, refer to the Dual Injector User's
Manual (P/N 221-40483).
Table 3-8
Corresponding AOC
Other Parameter on FUNCTION Description Setting Range
Parameter on GC Value
GC
Alloc F50 Specifies the 0-8 0
sample allocation in
DUAL mode. (Note 1)
Use the Same Param F51 Specifies how to set 0: Set on SUB 0
the parameters for injector.
the SUB injector in 1: Use MAIN
DUAL mode. parameters.
Note 1) This FUNCTION uses the same sample vial for both the MAIN and SUB injectors in DUAL mode.
These functions specify events such as RAM initialization.
Table 3-9

∗ F60 Initializes the RAM (executed when "1" is 0, 1 (Initialize) 0
set). (Note 1)
∗ F61 Displays the ROM version (XX.X). Cannot be Ver No.
modified.
∗ F62 Specifies which injector keyboard to 0: Set on SUB 0
operate for setting the SUB injector 1: Set on MAIN
∗ F63
parameters.
Specifies which injector receives 0: Receive on 0
3
transmitted commands. MAIN
1: Receive on
SUB (via MAIN
injector)
After normal start-up, setting F60 to "001" initializes the values for FUNCTION 01 - 13. To initialize
all the values, turn on the autoinjector while pressing the [SAMPLE WASH] key and set F60 to
"001".
@ NOTE
For GC-2010/2010Plus/2014/2025, when the gas chromatograph and autoinjector are linked after
the F60 initialization, the autoinjector parameters are updated to those set on the GC.
3 Operation
These functions set the transmission parameters.
@ NOTE
Except FUNCTION 84, all changed parameters become effective after restarting the autoinjector.
Table 3-10

∗ F80 CH1 baud rate 0: NOT USE 2
1: 1200
2: 2400
3: 4800
4: 9600
∗ F81 CH1 parity during simple procedure 0: NON 0
transmission 1: EVEN
∗ F82 CH1 stop bit (1/2) during simple procedure 0: 1bit 1
transmission 1: 2bit
∗ F83 CH1 protocol 0: Simple 1
procedure
1: LEVEL2
∗ F84 Simple procedure transmission format 0: Normal 1
(Note 1) 1: AOC-17
compatible
∗ F85 CH2 baud rate (Note 2) 0: NOT USE 0
1: 1200
2: 2400
3: 4800
4: 9600
∗ F86 (DO NOT change this parameter.) 0
Note 1) The AOC-20 can be used in AOC-17 mode. AOC-17 compatible mode is selected by setting FUNCTION
84 to "001" (the default value). Then set FUNCTION 83 to "000" (transmission protocol: "simple
procedure"). When AOC-17 compatible mode is selected, valid parameter ranges for the AOC-20 become
the same as those of the AOC-17. This is useful when upgrading from an AOC-17 autoinjector, because
injection conditions created for the AOC-17 from software that supports only the AOC-17, can still be used.
0: Normal mode ..................................... numerical values consist of three digits.
1: AOC-17 compatible mode ................. numerical values consist of two digits.
Note 2) CH2 is fixed at LEVEL 2, EVEN, STOP 1. Only the baud rate can be changed.
These functions specify features not categorized above.
Table 3-11

∗ F90 Specifies ON/OFF of the auto-stop function 0: OFF 1
(Note 1) 1: ON
∗ F91 Specifies the READY signal polarity. 0: OPEN 0
1: CLOSE
∗ F92 Specifies the number of injector 0: Single 0
automatically. (DO NOT change this 1: Dual
3
parameter.)
Rack F93 Specifies the rack type. 0: Short 0
1: Long
Rack Position F94 Specifies the rack position when using the 0-2 1
autosampler. (Note 2)
∗ F95 Specifies the injector in DUAL mode 0: MAIN 0
automatically. (DO NOT change this 1: SUB
parameter.)
∗ F96 (DO NOT change this parameter.) 0
∗ F97 (DO NOT change this parameter.) (Note 3) 0, 1 0
Note 1) Stops the second and subsequent injections, when the GC READY signal is "OPEN" even though the GC
is not READY (e.g. when the READY signal cable is cut).
Note 2) When using the autosampler, specifies which injection port on the GC the autoinjector is installed. (See
Figure 3-4.)
GC-14A/B, GC-15A, GC-16A, GC-2014, and GC-2025 ...........................Position "0"
GC-17Aver.1/2/3, GC-1700, GC-18A, GC-2010, and GC-2010Plus ........Position "1" or "2"
Note 3) Set to [1] only when using the DUAL injector without the autosampler, which disables the autosampler
regardless of the "Use of the Sampler" (F40) setting.
GC-2014 GC-2010
(GC-14A/B, GC-15A, GC-16A, GC-2025) (GC-17A ver.1/2/3, GC-1700, GC-18A, GC-2010Plus)
2
0
1
Figure 3-4
3 Operation
@ NOTE
The figure above shows the INJ port positions under the factory default setting.
Change the positions using the teaching function.
(See "2.7.4 Precautions for Using the Autosampler" P.70.)
The GC-2010/2010Plus with single injection port should be installed so that 2 INJ can be used
when "Rack Position" (FUNCTION 94) is set to [1] ("001").

3.2.9 GC-2010/2010Plus/2014/2025 Menus and Autoinjector Parameters

The following note the relationships between the autoinjector parameters and the AOC parameters
displayed with the GC-2010/2010Plus/2014/2025 [OPTION] key.
AOC Parameters
Sample Wash SAMPLE WASH (When the injection mode F07 is "0")
Number of Injection NUMBER OF INJECTIONS
Sample Size SAMPLE SIZE
Pre Solvent Wash F01 (When the injection mode F07 is "0")
SAMPLE WASH (When the injection mode F07 is other than "0", and F21 is
"1")
Solvent Wash SOLVENT WASH 3
Pumping F02
Viscosity F03
Dwell Time F04
Inj. Speed (Plunger) F05
Use the Same Param F51
(Only for MAIN injector in Dual injection mode)
AOC POWER None (Use the power switch on the external power supply unit.)
PF Menus
Start START
Stop STOP
Priority F10
OtherPara None (window switching key)
Sampler None (window switching key)
Reset RESET
Upload None (Used for data transfer from AOC to GC.)
Print None (Used for outputting AOC parameters on Chromatopac.)
Next None (window switching key)
AOC Priority Analysis

Injection Sample F10
PF Menus
Set [ENTER] key after setting F10

3 Operation
AOC Other Parameters

Inj. Speed (Syringe) F06
Using 3 Solvent Vials F28
Solvent Select F08
Air Suction F20
Plunger Suction Speed F22
Speed of Plunger F23
Syringe Height (↑) F25
Syringe Height (↓) F26
Syringe Height (Inj) F27
Multi-Inj. F33
Kinds of Vials F29
Rack F93
Kinds of the Syringe F34
Suction Volume for Washing F24
Rack Position F94
PF Menus
Inj Mode F07
Injection Mode
0 Sample + (Air)
1 Solvent + Air + Sample + (Air)
2 Solvent + Sample + (Air)
3 Solvent + Air + Standard (Internal standard) + Air + Sample + (Air)
4 Solvent + Standard (Internal standard) + Sample + (Air)
PF Menus
F21 F21
Autosampler
Use of the Sampler F40
Use of the Sub AOC F41
Bar Code Reader F42
PF Menus
Alloc F50

<Items which do not have corresponding AOC parameters, etc. on GC-2010/2010Plus/2014/2025>
Key
MONITOR
FUNCTION
F11 INJECTION SAMPLE
F12 FINAL SAMPLE
F13 INJECTION SAMPLE2
F30 Checking vial return position
F31 Analysis time (time before the next injection)
F32 Analysis start time 3
F35 Aspiration volume of the solvent in solvent flush mode
F60 RAM initialization
F61 ROM version display
F62 Setting the SUB injector parameter by the MAIN injector key operation
F63 Transmit commands to the SUB injector via the MAIN injector
F80 CH1 baud rate
F81 CH1 parity during simple procedure transmission
F82 CH1 stop bit (1/2) during simple procedure transmission
F83 CH1 protocol
F84 Simple procedure transmission format
F85 CH2 baud rate
F90 Auto-stop function
F91 READY polarity
F92 Injector: Single/Dual
F95 In Dual mode: MAIN/SUB
@ NOTE
Specifying batch schedules allows the same operations as FUNCTION 11 to 13. To use other functions,
operate the autoinjector keypad.

3 Operation
3.3 Sequential Analyses and Injection Modes

The autoinjector can be used as a sample injector for sequential analyses. It also supports various
injection modes, where you can freely change the parameters to create the optimum injection conditions
for different samples. This section describes the operation for various injection modes, such as sequential
analyses and solvent flush method.

3.3.1 Sequential Analyses
3.3.1.1 Preparation
Before performing sequential analyses, it is first necessary to configure the instrument, GC, and
peripherals as shown in Figure 3-5 to Figure 3-8. For more information on the connection procedure, refer
to the appropriate operation manuals.
AOC-20i
RS-232C cable READY/START cable 3
GC-2010
GCsolution
RS-232C cable for AOC AOC cable
Figure 3-5 Connection Example (Using GCsolution): Built-in Power Type (Back view)
AOC-20i
Chromatopac signal cable READY/START cable
GC-2010
Chromatopac
RS-232C cable for AOC AOC cable
Figure 3-6 Connection Example (NOT Using GCsolution): Built-in Power Type (Back view)

3 Operation
AOC-20i
CBM-102 Chromatopac signal cable
AOC cable
GC-17A
GCsolution
External power
supply unit
Optical link cable
RS-232C cable Optical link cable READY/START cable
Figure 3-7 Connection Example (Using GCsolution): External Power Type (Back view)
AOC-20i
Chromatopac signal cable
AOC cable
GC-17A
Chromatopac External power

supply unit
PAC START cable READY/START cable
Figure 3-8 Connection Example (NOT Using GCsolution): External Power Type (Back view)

If the autoinjector is connected as shown in Figure 3-5 to Figure 3-8, two-way communication is made
possible. A START signal is sent to the gas chromatograph when the sample is injected, starting the gas
chromatograph and data processor. When the analysis is complete and the gas chromatograph becomes
READY, the GC sends a READY signal to the autoinjector, which then begins its next injection.
If the READY cable connecting the gas chromatograph and the autoinjector is not connected, the injector
will not perform the subsequent injections.
Additionally, the autoinjector will not perform the subsequent injections if the gas chromatograph was not
programmed correctly. A GC temperature program or time program is required for a READY signal to be
sent.
• Temperature Programmed Analysis

The GC temperature programmed analyses are performed in the following sequence:
1 The gas chromatograph goes into the READY status, when the column oven temperature decreases 3
to the initial temperature after the temperature program is complete.
2 The READY signal is output to the autoinjector.
3 The autoinjector starts the next injection operation.
For GC-2010, 2010Plus, 2014, 2025, 17A, 1700 and 18A, specify an equilibration time (EQUIB TIME). After
completing the temperature program, the GC pauses to generate a READY signal for the specified time after
the column oven temperature decreases to the initial temperature. This allows the autoinjector to start
injections when the column temperature is stable at its initial temperature, which improves the repetition
reproducibility. (Default value on GC: 3 min.)
TEMP Equilibrium time
T2
T1
0 READY TIME
Figure 3-9 Temperature program with equilibration time

3 Operation
Note that the equilibration time cannot be set for GC-14A/B, 15A, and 16A. To improve the reproducibility
as mentioned above, change the temperature program as shown in Figure 3-10 to that in Figure 3-11. In
this case, set the second stage temperature rate to a value larger than "0". This allows the GC to become
READY when the column oven temperature is stable at its initial temperature, at which point the
autoinjector starts the next injection.
TEMP
T2
T1
0 READY TIME
Figure 3-10 1-step temperature program
TEMP Final time of the second stage
T2
T1
0 READY TIME
Figure 3-11 2-step temperature program (Final Temp is T1)
• Isothermal Analysis
When using GC-17A, 1700, 18A, 2010, 2010Plus, 2014, and 2025, an isothermal program can be set by
specifying initial temperature and time in the column temperature program, and setting the first stage
temperature rate to "0". ([END] is displayed for GC-2010/2010Plus/2014/2025.) By doing so, the GC does
not generate a READY signal at the set initial time since the temperature program is running, and the
autoinjector does not start the next injection.
Note that for GC-14A/B, 15A, and 16A, the isothermal program cannot be set only by specifying the initial
temperature and time. Be sure to set the first stage temperature rate to a value other than [0], and set the
first stage end temperature to the same value as the initial temperature. Additionally, the time values must be
set to satisfy the next equation: [Initial time + End time] = [Analysis time].
When using GC-14A/B, 15A, and 16A, a time program with a STOP time must be used to specify the
analysis run time. The GC becomes READY after the specified time for the time program elapses, and
generates a READY signal to trigger the next injection.
For example, to set 5 minutes of analysis time for the GC-14A or -14B, perform the following key operations
on the GC keypad:

Table 3-12
Key Operation Display
1 CITP Display continues to show the previous parameter (in this case, column
SHIFT.D oven temperature). S.DOWN light on.
2 TIME? Program time requested. DIALOG light on (dialog in progress).

7
S.DOWN light out.
PROG
3 5 Function set to 5 minutes after analysis start.

5
4 FUNCVL? Function requested.

ENT
5 0 Time program parameter (STOP time) set.
6
STOP
3
0
7 TIME? Program time requested for next step.

ENT
8 S.DOWN light on.

SHIFT.D
9 Program complete. S.DOWN, DIALOG lights off.

ESCP
When using GC-17A, 1700, 18A, 2010, 2010Plus, 2014, 2025, the analysis run time can also be specified
using the time program. For details, refer to the instruction manual of the gas chromatograph.
@ NOTE
When both a temperature and time program are loaded, the gas chromatograph reverts to the READY state
when the longer of the two programs ends. For example, if STOP time settings for the temperature program
and time program are five minutes and ten minutes, respectively, the READY signal is not generated until
the end of the time program (ten minutes), even though the oven temperature has already returned to the
initial temperature.
@ NOTE
When the gas chromatograph is READY, the autoinjector starts injection. The data processor should also be
READY when injection starts. For this reason, the gas chromatograph STOP time setting should be longer
than the STOP time setting for the data processor. (For Chromatopac, add the time to finish printing results
to the STOP.TIME setting.)

3 Operation
3.3.1.2 Operation
@ NOTE
When using GCsolution, CLASS-GC10, or Chromatopac GC network, operate the computer or chromatopac
to start the autoinjector.
1 Place the vials in the rack.

Since all of the samples placed in the rack will be analyzed, remove any samples not requiring analysis.
Then, turn the power on and set the parameters.
2 Start the autoinjector.

Each of the samples is injected the number of times specified by the NUMBER OF INJECTIONS
parameter (For GC-2010/2010Plus/2014/2025 [Number of Injection] in [AOC Parameters] on GC).
If the [RESET] key (For GC-2010/2010Plus/2014/2025, [Reset] (PF menu) in [AOC Parameters] on GC)
is pressed during the analysis, all information regarding number of analyses completed on the samples is
reset. When [START] is then pressed, the analysis restarts from the first sample.
If the [STOP] key (For GC-2010/2010Plus/2014/2025, [Stop] (PF menu) in [AOC Parameters] on GC) is
pressed during an analysis, the autoinjector suspends operation, but no information is reset. Pressing the
[START] key resumes the injection process from the sequence next to the interrupted sequence.
@ NOTE
During GC analysis, the autoinjector RUN light blinks. The autoinjector pauses until the GC sends its
READY signal. If the [STOP] key is pressed, the injection process is interrupted, and the RUN light
goes out. To restart the process, press [START].
3 When all of the samples have been analyzed, the autoinjector returns to the home
position.
@ NOTE
When the NUMBER OF INJECTIONS parameter is changed during a sequence, the new setting will
take effect starting with the next sample.
For example, if the setting is changed from 2 to 5 while sample No. 1 is being analyzed, sample
No. 1 will be analyzed twice. The new setting will take effect on the analysis of the next sample, and
sample No. 2 will be analyzed 5 times.

3.3.2 Priority Analysis

During continuous analysis, the autoinjector injects samples in ascending sample number order. The order
of analysis is determined by the placement of the sample vials. However, the following settings allow you to
skip to a certain sample or analyze preferentially only certain samples.
For GC-2010/2010Plus/2014/2025
Priority Analysis
When the autoinjector is operating, [Priority] (PF menu) is displayed in the gas chromatograph [AOC
Parameters] window.
Press this menu to display the window for specifying priority samples.
Enter the sample No. to preferentially inject, then [Set] (PF menu) to start the priority analysis.
After the priority analysis, the autoinjector resumes analyses from the next sample to the priority samples.
For example, if the analysis of sample No. 2 is interrupted for the priority analysis of sample No. 4, sample
3
No. 5 will be analyzed after completing the analysis of sample No. 4.
For details on the procedure, refer to the section "AOC priority analysis" in the instruction manual of the
gas chromatograph.
@ NOTE
If a parameter for priority analysis is changed before its execution, the new parameter is applied to the
priority analysis. If "0" is set, the priority analysis will be canceled.
@ NOTE
If a priority analysis is set during a repeated analysis of the same sample (i.e., "Number of Injection" is set to
2 or more), the priority analysis will be executed after the current injection.
@ NOTE
DO NOT set a priority sample while the autoinjector is actively functioning.
If you do, a new injection sequence for the specified priority sample is started during the current sequence,
which may cause a problem in that the syringe is not washed by the priority sample. To avoid this, be sure to
set a priority analysis after the autoinjector completes the current injection sequence (i.e., during standby for
the next injection).
(1) FUNCTION 10 (PRIORITY SAMPLE)
Example: Sample in rack position No. 2 is analyzed before

Table 3-13
Key operation Display
1 FUNCTION Example) Displays the last setting. (In this example, FUNCTION 03.)
F03
2 Arrow key F10 Press the arrow keys until "F10" displays.
3 ENTER 000 The current FUNCTION 10 set value is displayed. (A value of 0 indicates
no PRIORITY SAMPLE.)
4 Arrow key 002 Pressing the right arrow key twice displays "002".
5 ENTER 002 Sample No. 2 is set as a PRIORITY SAMPLE.
• In this example, the priority sample is set up before the [START] key is pressed. Sample No. 2 will
be the first sample injected, even if a sample vial is in position 1. Once executed, the PRIORITY
SAMPLE setting returns to "000".

3 Operation
• If a priority analysis is executed during a repeated analysis, the autoinjector resumes analyses
from the next sample to the priority samples.
@ NOTE
Once a PRIORITY SAMPLE setting has been made, it can be overridden before it is executed by
making another setting. For example, once sample No. 3 has been set as a PRIORITY SAMPLE, the
setting can be canceled by then specifying 0 as the PRIORITY SAMPLE.
@ NOTE
When a PRIORITY SAMPLE is scheduled during repeated analysis of the same sample (NUMBER
OF INJECTIONS set to 2 or more), the PRIORITY SAMPLE will be executed at the next injection.
@ NOTE
Do not set a priority sample when the autoinjector is actively functioning. Otherwise, all the rinses
may not be completed before the syringe moves to the priority sample. Make the priority setting
during an analysis or when the injector is not in use.
(2) FUNCTION 11 (INJECTION SAMPLE)

Set this parameter to analyze only the sample number specified, and no other samples. After the
priority sample is analyzed, the sequence ends.
Then, the set value of FUNCTION 11 automatically returns to "000".
(3) FUNCTION 12 (FINAL SAMPLE)

Set this parameter to analyze a priority set of samples. The sample number specified will be the last
sample analyzed. The sequence continues until the specified sample number is reached. That
sample is injected, and the sequence stops.
@ NOTE
When using GC-2010/2010Plus/2014/2025, batch schedules can be substituted for the FUNCTION 10-12
(features available when the autoinjector is on hold). For details, refer to the section "Batch Schedule" in the
instruction manual of the gas chromatograph.

3.3.3 Injection Modes
 Solvent Flush Mode

When FUNCTION 07 is set to "001" - "004" (For GC-2010/2010Plus/2014/2025, when [Inj Mode (PF
menu)] is set to [1] - [4] in [AOC Other Parameters] on GC), the autoinjector uses the solvent flush injection
method. (FUNCTION "003" and "004" are available only when using an autosampler.)
First set FUNCTION 07 ([Inj Mode] for GC-2010/2010Plus/2014/2025), and then set other injection
parameters.
Figure 3-12
In the solvent flush method, as shown in Figure 3-13, the autoinjector draws solvent, air, and sample, in
that order, into the syringe. After injection, solvent, not sample, is left in the syringe needle. Since the
plunger completely expels the sample, all of the sample vaporizes at the same time, reducing
discrimination.
The solvent flush method is recommended when analyzing compounds with a wide boiling point range.
Solvent
Sample
Air
Figure 3-13
The following Figures show the sample aspiration patterns in 3 injection modes with FUNCTION 07.
Sample
Figure 3-14 FUNCTION 07 "000" (Inj Mode: "0") (Normal method)

3 Operation
Sample Air (0.5 μl) Solvent (1 μl)
Figure 3-15 FUNCTION 07 "001" (Inj Mode: "1")
Sample Solvent (1 μl)
Figure 3-16 FUNCTION 07 "002" (Inj Mode: "2")
@ NOTE
It is recommended to select 10% (i.e., 1 μl of solvent) since the needle volume of the standard 10 μl syringe
is 0.6 μl.
The wash solvent is used for solvent flush, normally. However, when FUNCTION 28 is set to "001" (three
solvent positions are used), or for GC-2010/2010Plus/2014/2025, when [Using 3 Solvent Vials] is set to [Yes]
in the GC [AOC Other Parameters] window, the solvent at position C (the least contaminated) will be used
for solvent flush. (See Figure 3-17.) In this mode, the maximum number of samples that can be analyzed is
1 for the short rack, and 7 for the long rack. (Position 1 in Figure 3-17 is only for 4 ml vials. When 4 ml vials
are used as sample vials, the maximum number of sample vials is 2 for the short rack and 8 for the long
rack). However, 4 ml vial rack for autosampler (optional, P/N S221-45182) is separately required.
Positions for sample vials 1
C Waste liquid B Waste A Waste

(Positions for sample vials) vial liquid vial liquid vial
Solvent Vial
∗See note. Solvent Vial Solvent Vial
Figure 3-17
When using the solvent flush injection mode (FUNCTION 07 is set to "001" - "004", or [Inj Mode] is set to
[1] - [4] for GC-2010/2010Plus/2014/2025), the parameter upper limit and set items change as follows:
(1) SAMPLE SIZE ([Sample Size] for GC-2010/2010Plus/2014/2025)

The upper limit of sample size is reduced to 50% of the syringe volume specified in [Kinds of the
Syringe] in [AOC Other Parameters]. (Normally, 80%)

(2) For GC-2010/2010Plus/2014/2025

The following parameters in the [AOC Parameters] window become invalid.
Sample Wash
Pre Solvent Wash
In the solvent flush mode, the sample wash of the syringe is not performed.
The solvent wash of the syringe is performed before and after injections the number of times set in
"Solvent Wash".
However, if F21 (PF2) value is set to [1] with FUNCTION 21 (Injection Mode), it also becomes
possible to specify "Pre Solvent Wash".
(3) For Other Gas Chromatographs

SAMPLE WASH and SOLVENT WASH
The sample wash of the syringe is not performed regardless of the set value in SAMPLE WASH.
3
The solvent wash before injections is performed as specified number of times in SOLVENT WASH.
However, if FUCNTION21 is set to "001", the solvent wash is performed before injections the
number of times set by SAMPLE WASH, and after injections the number of times set by SOLVENT
WASH.
 Large Volume Injection Mode (Low-speed Injection Method)

If used with the optional PTV (Programmed Temperature Vaporizer) injection port, GC-2010/2010Plus and
GC-17A Ver.1/2/3 can handle injection volumes of several thousand microliters; however, it is necessary to
slow down the speed of injection as the amount of sample increases. In this method, quartz wool or column
packing material is placed in a PTV glass insert and used as a "pre-column". The target compounds are
trapped and concentrated in the pre-column, while the solvent elutes. After the solvent has eluted from the
pre-column, the target compounds are introduced onto the column.
When using the optional large volume syringe (50 μl or 250 μl), set FUNCTION 34 (For GC-2010/
2010Plus/2014/2025, [Kinds of the Syringe] in [AOC Other Parameters] on GC) as follows to set its
suitable plunger/syringe speed. The plunger speed during syringe wash and pumping is thus automatically
optimized for the large volume syringe. In addition, the plunger injection speed [High] is shifted to [Medium]
for 10 μl syringe when using 50 μl syringe, and to [Low] when using 250 μl syringe. To set even slower
speeds for particular solvents and analysis conditions, use FUNCTION 05 (For GC-2010/2010Plus/2014/
2025, [Inj. Speed (Plunger)] in [AOC Parameters] on GC) to set [Medium] and [Low] speed on each syringe
size.
Table 3-14
Syringe FUNCTION 34 setting

50 μl syringe (P/N S221-45243) 001
250 μl syringe (P/N S221-45244) 002
To inject volumes larger than the syringe capacity, use FUNCTION 33 (For GC-2010/2010Plus/2014/2025,
[Multi-Inj.] in [AOC Other Parameters] on GC) to increase the number of syringe injections after which the
GC receives the START signal from the autoinjector. For example, when using the large volume 250 μl
syringe, although 200 μl (80% of full scale) is the maximum volume for a single injection, setting
FUNCTION 33 to "005" (For GC-GC-2010/2010Plus/2014/2025, [Multi-Inj.] to [5]) starts the GC analysis
after five injections, so that a total of 1000 μl is injected (200 μl × 5 = 1000 μl).

3 Operation
@ NOTE
Possible injection volumes for CLASS-GC10 range from 0.1 μl to 8 μl. When using a large volume syringe,
injection volumes need to be converted as follows:
10 μl syringe: 0.1 μl - 8 μl (1 - 80% of 10 μl)
↓
Example: When injecting 20 μl with the 50 μl syringe:

20/50 × 100 = 40%
Therefore, set the injection volume to [4]. (40% of 10 μl)
@ NOTE
When using the standard syringe (10 μl), be sure to set FUNCTION 34 to "000" ([Kinds of the Syringe] to
[10 μl] for GC-2010/2010Plus/2014/2025). Otherwise, poor reproducibility may result.
 Aspirating Air after Drawing Sample

To aspirate 1 μl of air after drawing the sample, set FUNCTION 20 to "001". (For GC-2010/2010Plus/2014/
2025, set [Air Suction] to [Yes] in [AOC Other Parameters] on GC.) Using this injection method decreases
the amount of time the sample remains in the hot injection port, compared to the regular injection method.
This method reduces discrimination when analyzing components with a wide boiling point range.

3.4 Autosampler Operation

In principle, control the autosampler via the gas chromatograph main unit. (Use the autoinjector keypad
occasionally.) The procedure for setting parameters is the same as for the autoinjector. This section
describes the autosampler parameter settings which differ from those of the autoinjector.
3.4.1 Preparation
(1) Setting sample gripping position
For GC-2010/2010Plus/2014/2025
Using [Rack Position] in the [AOC Other Parameters] window, specify the position of the GC
injection port where the autoinjector is installed. 3
GC-2010/2010Plus Rear (=Standard position): 2 (The autosampler can be adjusted for use under
the setting of "1".)
GC-2010/2010Plus Front: 1
GC-2014/2025: 0 (Specify [1] when using the optional mounting bracket (for AOC-20) to use the
injection port located at far left. See "2.1.2 Mounting Spacers on GC-2014/GC-14A/B" P.23.)
Then set [Use of the Sampler] to [Use] in the [Sampler] window.
When using only the autoinjector (without the autosampler), set [Use of the Sampler] to [Not Use].
For GC-17A Ver.1/2/3, GC-1700, or GC-18A

Using FUNCTION 94, specify the position of the GC injection port where the autoinjector is installed.
When the autoinjector is installed over the injection port behind the standard position, set
FUNCTION 94 to "002". (See Figure 3-4.)
Then set FUNCTION 40 to "001". This places the autoinjector in autosampler mode. (This setting is
saved when the power is turned OFF.)
When using only the autoinjector (without the autosampler), set FUNCTION 40 to "000" (The
autosampler is not used).
For GC-14A/B
When using the autosampler with a GC-14A/B, set FUNCTION 94 to "000".
For both the GC-14 and -17 series, set FUNCTION 40 to "001". This places the autoinjector in
autosampler mode. (This setting is saved when the power is turned OFF.)
The autoinjector can be used without the autosampler; set FUNCTION 40 to "000".
@ NOTE
If the position of the autosampler arm in relation to the vial is not correct at gripping position, teach the
autosampler the correct position (teaching function). (See "2.7.5 Adjusting Sample Gripping Position
(Teaching)" P.70.)

3 Operation
(2) Placing solvent vials in the autoinjector rack

When the autosampler is in use, a maximum of 150 sample vials can be used. This allows for 3 vials
each for solvent and waste liquid. The vials are transferred to position 2 during every analysis, so do
not place vials in this position. (See Figure 3-18.)
Place the solvent for solvent flush or standard sample vial in the position 1. (See "3.4.6 Solvent
Flush Injection Mode" P.124.)
Waste liquid
vial B
Solvent vial
Solvent vial Solvent vial

Sample vial position
C A
Waste liquid Waste liquid
vial vial
Figure 3-18
(3) Placing vials in the autosampler tray

The autosampler tray consists of 6 vial racks (which can each hold twenty-five 1.5 ml vials or sixteen
4 ml vials). Place the vials in the vial rack(s) to be used. Numbers are assigned to the vial positions
as shown in Figure 2-55 and Figure 2-56.
To specify the sample vial size, use FUNCTION 29. ("000" for 1.5 ml and "001" for 4 ml.)
When using GC-2010/2010Plus/2014/2025, set [Kinds of Vials] to 1.5 ml or 4 ml in the [AOC Other
Parameters] window.

It is not necessary to place empty racks in the sample tray. Place only the racks with samples. The
racks do not have to be in sequential order. For example, when 1.5 ml vials are placed as shown in
Figure 3-19, the vial rack at position 1 holds vial numbers 1 to 25. At position 4, the rack holds vial
numbers 26 to 50, and at position 5, numbers 51 to 75. If a rack is loaded for different size vials than
the size set by FUNCTION 29 ([Kinds of Vials] for GC-2010/2010Plus/2014/2025), the rack is
ignored.
Figure 3-19
Set vials in the sample rack in sequential order from position 1. The first vial used for analysis is the
vial at position 1. See Figure 3-20 for a diagram of the position of the sample vials.
Figure 3-20

3 Operation
@ NOTE
1 After pressing the autoinjector [START] key, the autosampler detects the position of the sample
rack on the tray. In this case, vial numbers are assigned to only vials in that sample rack.
To avoid malfunction, DO NOT remove the rack after sample vial numbers are assigned.
2 If you want to add racks after analysis has been started, add them behind the rack containing
the vial currently being analyzed. Numbers will be reassigned to the vials. Racks added in front
of the current rack will be ignored.
As shown in the following examples, if a position in the rack is left empty, analysis on that rack ends,
and the autosampler starts analysis on the next vial rack. If no vial is placed in the starting position
of that rack, operation stops.
For example, when 1.5 ml vials are positioned as shown in Figure 3-21 (the black circles indicate
vials), samples 1 through 18 are analyzed. Next, the autosampler searches for, but does not detect,
a vial in position 19. It then searches for a vial in position 26 (the first vial of the next rack). If the
autosampler does not detect a vial there, it stops operation.
Figure 3-21

When the vials are positioned as shown in Figure 3-22, after analyzing samples in positions 1 to 18,
the autosampler does not detect a vial in position 19. It checks in position 26, finds a vial there, and
analyzes the samples in positions 26 to 31. The autosampler finds no vial in position 32, detects no
vial in position 51, then stops.
Figure 3-22
When the vials are placed as shown in Figure 3-23, after analyzing samples in positions 1 to 18, the
autosampler does not detect a vial in position 19. It skips the vials in positions 20 to 25, and
searches for a vial at position 26. Detecting no vial in this position, the autosampler stops operation,
skipping the samples in positions 51 to 53. Only the samples in positions 1 to 18 are analyzed.
Figure 3-23
As shown in the examples above, if a position in the rack is left empty, analysis on that rack ends,
and the autosampler starts analysis on the next vial rack. If no vial is placed in the starting position
of that rack, operation stops.

3 Operation
3.4.2 Power ON
The autosampler shares the power supply with the autoinjector.
Turn ON the switch on the power supply unit.
For GC-2010/2010Plus/2014/2025, you can toggle the power ON/OFF in [AOC POWER] in the gas
chromatograph [AOC Parameters] window. When the power is turned ON, the power light starts blinking
green, the initial operation is performed (illuminates orange), the autosampler arm moves to its home
position, and the autosampler goes into stand-by.
Arm
Gripper
Vial rack
Tray
Power light
Figure 3-24
3.4.3 Power Light

The color of the power light indicates the operational status of the autosampler. When power is supplied
and the arm moves to its home position, the light blinks green. In home position, the light is steady green.
While the autosampler is operating, the light is orange, and when an error occurs, it is red.
@ NOTE
When the light is orange, the autosampler is operating, so do not touch the arm. Applying excessive force
may break the motor.
@ NOTE
Perform all key operations when the autosampler is standing by after initial operations are completed.
(Except if an error occurs.)

3.4.4 AOC Parameters

The parameters for controlling the autosampler are given below. For the setting procedure, see "3.2.8
FUNCTION Key" P.92.
• Injection Mode FUNCTION 07/ AOC Other Parameters PF2 (Inj Mode)
In solvent flush mode, the internal standard mode (3 and 4) is available. (See "3.4.6 Solvent Flush Injection
Mode" P.124.)
• Solvent Selection FUNCTION 08 Solvent Select

Specify which of the three solvents to use for solvent wash.
• Sample Vial Size FUNCTION 29/ AOC Other Parameters Kinds of Vials
Specify the sample vial size from 1.5 ml and 4 ml. This setting cannot be changed once analysis has begun.
The change made after START will be ignored. To change the size, press the [RESET] key and set the
parameter.
You can START and RESET analyses via the gas chromatograph display.
3
• Checking Vial Return Position FUNCTION 30
It is specified whether to check the rack position (to ensure that it is empty) before replacing a vial. "000"
indicates do not check, and "001" indicates check. Set this parameter with the autosampler keypad, even
when using GC-2010/2010Plus/2014/2025.
3.4.5 Priority Analysis

For GC-2010/2010Plus/2014/2025
(1) While the gas chromatograph is analyzing (i.e., the autoinjector is standing by), press [Priority]
(PF2) in the AOC parameter to display the priority analysis window.
(2) Specify the desired priority samples and press [Set] (PF2).
(3) After the analysis currently executed is complete, the autoinjector starts injecting the specified
priority samples.
(4) After the priority analysis is complete, the autoinjector starts injections from the sample next to the
one completed before interruption.
(5) The set values for priority analyses are reset to [0] after the completion of the analyses.

If a priority sample is specified (FUNCTION 10) before analysis starts, the first sample analyzed will be the
priority sample (instead of the sample in position 1). After the priority sample is analyzed, the value of
FUNCTION 10 returns to 0 (no priority sample).
When FUNCTION 10 is set while the GC is performing analysis (with the autoinjector on standby), after
completing the current analysis, the priority sample is analyzed. Analysis will then continue where it left off.

3 Operation
3.4.6 Solvent Flush Injection Mode

When using an autosampler, the solvent used to perform solvent flush injections is placed in a special
position in the rack. Because the rinse solvent is not used for solvent flush, solvent flush injections have
less chance of being contaminated.
Place the solvent for solvent flush injections in position 1 of the rack. (See Figure 3-25.)
Sample vial rack for autosampler
W S W S W S
S = Solvent
W = Waste liquid
Solvent for solvent flush (4 ml vial)
For sample
Figure 3-25
Figure 3-26 to Figure 3-29 shows each injection mode available through FUNCTION 07 ([Inj Mode] for GC-
2010/2010Plus/2014/2025) when an autosampler is used.
Sample Air (0.5 μl) Solvent for solvent flush (1 μl)
Figure 3-26 When FUNCTION 07 is "001" ([Inj Mode] is [1] for GC-2010/2010Plus/2014/2025)
Sample Solvent for solvent flush (1 μl)
Sample Air (0.5 μl) Air (0.5 μl) Solvent C (1 μl)
Standard sample (1 μl)
Sample Solvent C (1 μl)
Standard sample (1 μl)

Set FUNCTION 07 ([Inj Mode] for GC-2010/2010Plus/2014/2025) to "003" or "004" when performing
internal standard analysis and solvent flush injection mode. This automatically aspirates internal standard
and injects it along with the sample. The internal standard is placed in position 1 of the rack. The solvent for
solvent flush is then taken from the solvent in position C of the rack (Figure 3-30).
Sample vial case for sampler
W S W S W S
Sample
Standard sample (4 ml)
S = Solvent
W = Waste liquid 3
Figure 3-30
When injecting internal standard along with the sample, not only 1 μl but 0.5 μl can be selected for solvent
aspiration volume for solvent flush. To do so, set FUNCTION 35 using the autoinjector keypad.
0: 10% of syringe volume
1: 5% of syringe volume

3 Operation
3.5 Analysis Tips and Precautions

Pay attention to the following when analysis is performed with an autoinjector, gas chromatograph, and
data processor. (See also "7 To Achieve Good Analysis Reproducibility" P.155.)
(1) Set up analysis programs on GC and monitor cable connections
(a) Set up a gas chromatograph temperature, isothermal, or time program

The autoinjector starts the next injection after the temperature, isothermal, or time program (as
well as flow program for GC-17A Ver.1/2/3, GC-2010, GC-2010Plus, GC-2014 and GC-2025)
ends and the GC becomes READY. If no program was created, sequential analysis cannot be
executed. (See "3.3.1 Sequential Analyses" P.105.) If a sequence is attempted without
creating any of above-mentioned programs, the autoinjector stops and returns to home
position after injecting the first sample.
(b) Check the cables

If the autoinjector stops unexpectedly after an injection, check cable connections. If the cable
connecting the power supply box and the gas chromatograph is disconnected, or the power of
the gas chromatograph is turned OFF, the autoinjector will appear as if the [STOP] key had
been pressed.
This problem may also occur when a temperature, isothermal or time program is not set to the
gas chromatograph.
(2) Set the program time of the gas chromatograph longer than the data processor analysis time. (For
Chromatopac, add the printout time to STOP.TIME.)
The data processor has to be READY before the gas chromatograph in order to correctly follow the
sequence of [Sample injection → Gas chromatograph starts → Data processor starts]. Allow time for
any reports to print before starting the next injection, or the data of the next injection may not be
acquired.
(3) Be aware of vial capacity limitations

Remember the capacity limit of the solvent and the waste liquid vials when setting NUMBER OF
INJECTIONS ([Number of Injection] for GC-2010/2010Plus/2014/2025), SAMPLE WASH ([Sample
Wash]) or SOLVENT WASH ([Solvent Wash]). (See "2.4.2 Septum Replacement and Sample
Volume in Vials" P.48.)
(4) Replace the injection port septum frequently

The injection port septum should be replaced every 100 injections or fewer when the autoinjector is
used. Using a worn septum may cause injection port leaks and reproducibility deterioration.
Referring to "2.5 Replacing INJ Port Septum" P.57, replace the septum periodically.
(5) Inspect and maintain the syringe (See "7.1 Precautions on Use of the Microsyringe" P.155 and "7.2
Maintenance of the Microsyringe" P.156.)
When the plunger becomes stiff, the LED to the left of the [SAMPLE SIZE] key blinks as a warning
during analysis (when not in solvent flush mode). When the plunger becomes too stiff to move
normally, error "-03" displays and the autoinjector stops operation.

3.5 Analysis Tips and Precautions
(6) Monitor the injection amount

The volume of liquid sample that is normally injected into a GC is 1 to 2 μl. This volume expands
rapidly when the liquid vaporizes. The sample vaporized volume varies, depending on the solvent
type and its vaporized volume, as well as the GC analysis parameters (such as injection port
temperature, carrier gas flow rate, and inlet pressure). If the vaporized volume exceeds the capacity
of the injection port glass insert, analysis results will not be reproducible.
Other analysis reproducibility problems occur with a slow sample speed in injection port, such as in
splitless analyses using capillary column. Reproducibility problems may occur also when injection
volume is very low. In such a case, place the silica wool higher so that the syringe needle tip is
inserted into the wool during injections, which may allow small volume samples to vaporize
efficiently.
When performing splitless analyses with the GC-17A Ver.2/3, reproducibility may improve if the
pressure mode during the sampling time (split vent closed) is changed to front pressure mode. This
is especially effective for solvents with a large vapor volume (such as acetone and methanol). 3
To change the GC-17A Ver.2/3 splitless mode to front pressure mode, follow the table below.
Table 3-15
Key Operation Display Comment
1
FUNCTION NUMBER
__
2 Selects FUNCTION No.

FUNCTION NUMBER (18: Customization)
18 __
3
FUNCTION NUMBER
CUSTOMIZE 18 __
4 Displays current setting.

SPL1 SPLITLESS The default setting is for back pressure
F.PRESS B.PRESS __ mode.
5 Changes to front pressure mode.

SPL1 SPLITLESS
F.PRESS__ B.PRESS
6 Ends parameter setting.
FUNCTION ESCAPE

3 Operation
3.6 GCsolution
3.6.1 System Configuration
Select a type of the autosampler to be used under [Available Modules] in the [System Configuration]
window. Add it to the analytical line as a [Modules Used for Analysis].
AOC-20i only: AOC-20i

AOC-20i + AOC-20s: AOC-20i+s
Dual injection system (MAIN): AOC-20d (M)
Dual injection system (SUB): AOC-20d (S)
Figure 3-31
For details on the dual injection system, refer to the instruction manual of AOC-20 Dual Injection System
(P/N 221-40483).
Complete the required setting in the properties of the AOC unit icon under Analytical line.
Figure 3-32

3.6 GCsolution

Complete the parameter settings in the autosampler tab page in [Data Acquisition] - [Instrument Parameter
View].
Figure 3-33
Figure 3-34
@ NOTE
For details on the parameter settings, refer to the instruction manual of GCsolution.
For the setting procedures for GCMSsolution, refer to the instruction manual of GCMSsolution.

3 Operation
3.7 CLASS-GC10
3.7.1 System Configuration
Select the following items from the system configuration autosampler drop-down list.
AOC-20i with short rack: AOC-20i (short)
AOC-20i with long rack: AOC-20i (long)
AOC-20i + AOC-20S: AOC-20s
Dual injection system: AOC-20d
Figure 3-35 CLASS-GC10 System Configuration screen
∗ For a dual injection system, refer to the instruction manual of AOC-20 Dual Injection System (P/N 221-40483).

3.7 CLASS-GC10

Select [AUTOSAMPLER] from the [METHOD SETUP] menu, or the [AUTOSAMPLER] menu command in
the <GC SETTING> window, to open the <autosampler> window, and set up the parameters.
Figure 3-36 <Autosampler> window
The following parameters appear in this screen.

• # of Rinses with Sample: Sample rinse frequency
• # of Rinses with Solvent (pre): Solvent rinse frequency (before injection)
• Viscosity Comp. Time: Plunger hold time after sample aspiration (sec)
• Injection Speed: Plunger injection speed
• Syringe Speed: Syringe injection speed
• Inj. Port Dwell Time: Wait time after injection (sec)
• # of Rinses with Solvent (post): Solvent rinse frequency (after injection)
• Injection Mode: Injection mode (normal, solvent flush, etc.)
• Pumping Times: Number of pumpings
Specify the injection volume and the number of injections per sample, when registering samples or creating
the sample schedule.
@ NOTE
For setting up large volume sample injections, see "3.3.3 Injection Modes", "Large Volume Injection Mode
(Low-speed Injection Method)" P.115. The range of injections per sample is 0 - 10.
@ NOTE
The default values of [Injection Speed] and [Inj. Port Dwell Time] are [Medium] and [0.3 sec], respectively.
The recommended AOC-20 values are [High speed] and [0 sec].

3 Operation
3.8 CLASS-VP
3.8.1 Autoinjector/Autosampler Configuration
To configure CLASS-VP for the AOC-20 autoinjector and/or autosampler, choose Configuration from the
CLASS-VP Main Menu. Then select the Injection button. The autoinjector primary configuration screen is
displayed; choose Shimadzu AOC from the drop-down list. Click on Settings.
Figure 3-37
Choose the appropriate Comm. Port, Model, Vial Size, and Syringe Type from the drop-down list
selections. To test the connection, click on the Test button. Restart the program for the new configuration to
take effect. Please see the CLASS-VP manual for more information.
3.8.2 Pretreatment Setup Screen

From the [Batch] menu, select [Pretreatment Setup] to enter autoinjector parameters.
Figure 3-38
The parameter functions correspond to the autoinjector parameters described in "3.2 Display and Keypad
Functions" P.89. Set them up as you would on the autoinjector. Then, save the pretreatment file by
choosing [Save] from the [File menu]. The file is saved with an.aoc extension, which is then specified when
setting up the batch.
@ NOTE
When the autoinjector is controlled by CLASS-VP, FUNCTION 31 must be set to 655 min.

4 4 External Control
This section describes how to control the autoinjector externally.

When connecting the autoinjector and an external control device, the following two methods of
transmission can be used. (Set FUNCTION 83 to select transmission method.)
4
• Transmission by "Simple procedure" 4
This is the transmission method used for AOC-17 or AOC-14 communication. Use this method when
connecting a PC or Chromatopac (except for the C-R7A). Also, specify this method when creating new
programs or using programs created for the AOC-17 or AOC-14. 4
• "Level 2" transmission
4
This transmission method is used to connect the autoinjector to a C-R7A Chromatopac. This method is also
used for communication between the gas chromatograph and the C-R7A Chromatopac.
Either a fiber optic or RS-232C interface can be used for the external control connection. However, both
cannot be used at the same time. 4
The pin configuration for the RS-232C connector is shown below. It is the same configuration as that of
IBM PC/AT compatible computers. Use a commercially available null modem cable for an RS-232C
connection to external devices. 4
Table 4-1 RS-232C pin configuration
4
Pin No. Function
1
2
NC (Not used)
RxD (Received Data)
4
4
3 TxD (Transmitted Data)
4 DTR (Always ON)
5 Ground
6 NC (Not used) 4
7 RTS (Always ON)
8
9
NC (Not used)
NC (Not used)
4
4
4
4
4
4
4 External Control
4.1 Preparation
Make the following settings to prepare for the autoinjector transmission. Restart the unit for the settings to
take effect.
 Transmission by Simple Procedure

F80 ...................Baud rate. Leave the default value of 2.
F81 ...................Parity. Leave the default value of 0. (A value of 1 indicates even parity).
F82 ...................Stop bits. Leave the default value of 1.
F83 ...................CH1 protocol. Change to Simple procedure (0).
F84 ...................To remain compatible with the AOC-17 or AOC-14, leave the default value of 1; otherwise,
set to 0. See Note 1).
F86 ...................Leave the default value of 0.
Note 1) Depending on the setting for F84, the following transmission parameters are changed.
0: Numerical values consist of three digits
A response is made to RSET (reset command).
1: Numerical values consist of two digits (100 is transformed to "1*".)
The RSET (reset) command has no effect.
The parameter setting ranges become the same as those of the AOC-17.
A value larger than 100 cannot be sent when F84 is set to 1.
 Level 2 Transmission
F80 ....................Baud rate. Leave the default value of 2.
F81 ....................Setting not necessary
F83 ....................Leave the default value of 1.
F86 ....................Leave it as 0 (default value).
MODE setting of the Chromatopac (communication port parameter) .......... Change to 12917

4.2 External Control

The external control device communicates with the autoinjector as follows.
• Sends parameters to the autoinjector.
• Receives parameters or monitors the autoinjector status.
• Controls autoinjector operation (Start, Stop, Reset, etc.)
The Simple procedure method of sending/receiving commands differs from the Level 2 procedure.
Although a decimal number such as "00.3" can be displayed on the screen (as in a time or injection volume
setting), only whole integer values can be used for transmission. For example, transmit 003 instead of 00.3
and 100 instead of 10.0.
"4.2.1 Transmission by Simple Procedure" P.135 describes transmission by Simple procedure, and "4.2.2
Level 2 Transmission" P.138 describes Level 2 transmission.
"4.2.3 Command List" P.140 lists communication commands.
4.2.1 Transmission by Simple Procedure 4

When transmitting a command to the autoinjector, 3 kinds of modes are available: K, M, and S. These
codes are added to the beginning of a transmission command to distinguish each mode.
K: Setting mode (Setting parameters and Start, Stop, and Reset)
M: Actual value request mode (such as monitoring the sample number)
S: Setting value request mode (reading parameters)
4.2.1.1 Parameter Transmission (Setting)

Setting autoinjector parameters from an external device is illustrated below.
Autoinjector External control device

Parameter transmission

"AK CR " or "NK CR " returned
"CR" stands for "Carriage return."
Figure 4-1 Setting a parameter
The steps in Figure 4-1 are shown below.
1 Transmit "K" + Transmission command + " " + Parameter + CR to the autoinjector.

For example, the command for setting SAMPLE WASH to 3 is "KWRPT 003" + CHR$(0DH). Note that " "
means a space.
2 If the setting was successfully made, the autoinjector returns "AK CR ". If the setting
was not successfully made, then "NK CR " is returned.
@ NOTE
For the list of transmission commands, see "4.2.3 Command List" P.140.

4 External Control
When setting autoinjector parameters, use the format described in 1. Always designate parameters with 3
digits (for example, to set 3, use 003). However, use only 2 digits when FUNCTION 84 is set to "001".
If the setting was successfully completed, the autoinjector returns "AK CR ". If the setting was not
performed (mistaken transmission code, invalid parameter, etc.), "NK CR " is returned.
For an example, a BASIC program for setting the PUMPING frequency to 6 from a Chromatopac C-R7A or
C-R4A is shown below. (Port No. 3 is used.)
OPEN TRS 3 Port open
C$ = "KPUMP 006" + CHR$(0DH) Command for PUMPING 6 times
PRINT #3,C$; Transmission
INPUT #3,A$ Input response from the autoinjector
CLOSE TRS 3 Port close
@ NOTE
Be sure to add a semi-colon (;) at the end of the sentence when sending a command.
4.2.1.2 Parameter Reception and Status Monitoring

Reception of autoinjector parameters by an external control device is illustrated below.

Parameter transmission request

Parameter returned
Figure 4-2 Transmission and reception of a parameter
The steps in Figure 4-2 are described below.
1 Transmit "S" + Transmission command + " " + CR to the autoinjector.

(For example, the command for receiving the value for SAMPLE WASH is "SWRPT" + CHR$(0DH)).
2 The parameter is returned.

(However, if the command was sent improperly, "NK CR " is returned.)
(For example, when SAMPLE WASH is set to 2, "002 CR " is returned.)
@ NOTE
Use "M" at the beginning of a command, instead of an "S", to monitor operations (receive actual
values). See the command list in "4.2.3 Command List" P.140.

4.2.1.3 Controlling Injector Operation

To control the autoinjector (RESET, START, STOP) through an external device, follow the procedure below.
1 Transmit "K" + Transmission command + " " + CR to the autoinjector.

(For example, the command to start the autoinjector is "KSTRT" + "CHR$ (0DH).)
2 "AK CR " or "NK CR " is returned.
@ NOTE
The reset command is ignored when F84 is set to 1.
4.2.1.4 Transmission Precautions

(1) Three types of commands are available: K mode (setting mode), M mode (actual value request
mode), and S (set value request mode). Some commands can only be used for certain modes. See
the command list in "4.2.3 Command List" P.140.
4
(2) When a command is sent to the autoinjector, "AK CR ", "NK CR " or a parameter is returned. The
response is 3 bytes for AK and NK. For a parameter, it is 4 bytes. However, when FUNCTION 84 is
set to "001", the response of a parameter is 3 bytes (2 digits not including CR ), and there is no
response when the RESET command is sent.
(3) If a transmission error occurs, and the condition cannot be returned to normal, send CHR$(05H)
(ENQ command) to the autoinjector. CHR$(05H) resets the autoinjector transmission line, and "AK
CR " is returned.
(4) When " " (space) in the transmission command is replaced with "$", the autoinjector returns the
command along with the value. This makes it easy to distinguish to which command the response
corresponds.
For example:
Command to be transmitted Autoinjector
"SWRPT"+ CHR$ (0DH) "001 CR "
"SWRPT$"+ CHR$ (0DH) "WRPT = 001 CR "
"KSTRT"+ CHR$ (0DH) "AK CR "
"KSTRT$"+ CHR$ (0DH) "STRT = AK CR "
@ NOTE
Follow the procedures below to prevent transmission errors.
(5) When a command is transmitted to the autoinjector, make sure the response is returned from the
autoinjector before sending the next command.
(6) As in the program example shown in "4.2.1.1 Parameter Transmission (Setting)" P.135, be sure to
add a semi-colon (;) at the end of the sentence when sending a command. When a program is
executed without including the semi-colon, no carriage returns or line feeds occur.

4 External Control
4.2.2 Level 2 Transmission

When Level 2 transmission is used, transmission is performed with single commands instead of programs.
The following three kinds of modes are available.
K: Setting mode (Setting of parameters and START, STOP, and RESET commands.)
M: Actual value request mode (such as reading SAMPLE NUMBER)
G: Set value request mode (reading parameters)
4.2.2.1 Opening the Port

Before transmission can occur between the autoinjector and the Chromatopac, perform the following
operation on the C-R7A Win3 screen to open the port.
Be sure to insert a space between "OPEN" and "TRS" as well as "TRS" and the physical port number.
OPEN TRS 3
Physical port number
For the physical port number, refer to the GC network instruction manual.
4.2.2.2 Transmission of Parameters (Setting)

To change the autoinjector set values from the Chromatopac, perform the following operation on the Win 3
screen. (These are K mode operations.)
Example 1: Set the NUMBER OF INJECTIONS to 3.
REPT#3=3
Example 2: Set the VISCOSITY to 3 sec.
WTPP#3=30
Logical port number
For the logical port number, refer to the GC network instruction manual.
In Simple procedure transmission, the number must be transmitted in 3 digits, so that [1] becomes [001]
(or, when F84 is set to 1, in two digits). However, in Level 2 transmission, the number of digits is not taken
into consideration.
4.2.2.3 Reception of Parameters and Status Monitoring

To display set or monitored autoinjector values on the Chromatopac screen, perform the following
operations from the Win 3 screen.
Example 1: Display the number of solvent rinses. (SOLVENT WASH)
PRINT WMOD#3
Example 2: Print the SAMPLE NUMBER.
LPRINT ISNO#3M
Logical port number
Receiving the set value, as shown in Example 1, is a G mode operation.
Monitoring the status, as shown Example 2, is an M mode operation. Therefore, M is added to the
command.

4.2.2.4 Controlling the Autoinjector

To operate the autoinjector from the Chromatopac (START, STOP and RESET), perform the following
operation on the Win 3 screen (these are K mode operations).
Example 1: Start the autoinjector.
STRT#3
Example 2: Stop the autoinjector.
STOP#3
Example 3: Reset the autoinjector.
RSET#3
Logical port number
4.2.2.5 Closing the Port 4

Before turning OFF the power of either the autoinjector or Chromatopac, close the port. To close the port,
perform the following operation on the Chromatopac Win 3 screen.
Be sure to insert a space between "CLOSE" and "TRS" as well as "TRS" and the physical port number.
CLOSE TRS 3
Physical port number

4 External Control
4.2.3 Command List

• SAMU, SUBU, BARC commands
In G and S modes, the set values are returned as the response. The M mode response indicates whether
the unit is ready (a response of 1 indicates that the unit can be used.)
• RDYF command
1 is returned when waiting for analysis or START, and 0 otherwise.
• STRF
1 is returned when ready to start. 0 indicates an error.
Table 4-2
Command Default
Mode Contents Valid Range
Name Value
STRT K START
STOP K STOP
RSET K RESET
WRPT K, G, S SAMPLE WASH 000 - 099 002
WMOD K, G, S SOLVENT WASH 000 - 099 001
REPT K, G, S NUMBER OF INJECTIONS 001 - 099 001
IVOL K, G, S SAMPLE SIZE 001 - 080 010
WPRS K,G.S F01 PRE SOLVENT WASH 000 - 099 000
PUMP K, G, S F02 PUMPING 000 - 099 005
WTPP K, G, S F03 VISCOSITY 000 - 999 002
WAIT K, G, S F04 DWELL TIME 000 - 999 000
ISPD K, G, S F05 INJ SPEED (PLUNGER) 000 - 002 002
SSPD K, G, S F06 INJ SPEED (SYRINGE) 000 - 001 001
SAND K, G, S F07 INJECTION MODE 000 - 004 000
SOLV K, G, S F08 SOLVENT SELECT 000 - 003 000
SINT K, G, S F10 PRIORITY SAMPLE 000 - 150 000
SSNO K, G, S F11 INJECTION SAMPLE 000 - 150 000
FSAM K, G, S F12 FINAL SAMPLE 000 - 150 000
SNO2 K, G, S F13 INJECTION SAMPLE2 000 - 150 000
AAIR K, G, S F20 WITH/WITHOUT AIR ASPIRATION 000 - 001 000
WKEY K, G, S F21 KEY FOR SOLVENT WASH BEFORE 000 - 001 000
INJECTION
USPD K, G, S F22 PLUNGER ASPIRATION SPEED 000 - 002 002
DSPD K, G, S F23 SPEED TO LOWER PLUNGER 000 - 002 002
UVOL K, G, S F24 SUCTION VOLUME AT SAMPLE WASH AND 000 - 001 000
PUMPING
HIGH K, G, S F25 SYRINGE HEIGHT WHEN ASPIRATING (UP) 000 - 020 000
LOWS K, G, S F26 SYRING HEIGHT WHEN ASPIRATING (DOWN) 000 - 010 000
INJH K, G, S F27 SYRING HEIGHT ATINJECTION (UP) 000 - 022 000
SLMD K, G, S F28 3 SOLVENT VIALS IN USE 000 - 001 000
VIAL K, G, S F29 VIAL SIZE 000 - 001 000
CKTR K, G, S F30 WITH/WITHOUT TRAY CHECK 000 - 001 000

Command Default
Mode Contents Valid Range
Name Value
TANL K, G, S F31 ANALYSIS TIME 000 - 655 000
TSTR K, G, S F32 ANALYSIS START TIME 000 - 999 000
STRI K, G, S F33 NUMBER OF INJECTIONS PER ONE 001 - 099 001
ANALYSIS
LSYR K, G, S F34 SYRINGE SIZE 000 - 002 000
SVOL K, G, S F35 SOLVENT ASPIRATION VOLUME IN SOLVENT 000 - 001 000
FLUSH MODE 3, 4
SAMU K, G, S, M F40 USE/NOT USE AUTOSAMPLER 000 - 001 000
SUBU K, G, S, M F41 USE/NOT USE SUB AOC 000 - 001 000
BARC K, G, S, M F42 USE/NOT USE BAR-CODE READER 000 - 001 000
SPMD K, G, S F50 SAMPLE DISTRIBUTION OF DUAL AOC 000 - 008 000
PAR1 K, G, S F51 OPERATING SUB-INJECTOR BY
PARAMETERS OF MAIN-INJECTOR
000 - 001 000
4
SET2 K, G, S F63 SEND PARAMETER COMMAND TO SUB AOC 000 - 001 000
GLPM K, G, S VALIDATION MODE SETTING 000 - 001 000
CH1B K, G, S F80 CH1 BAUD RATE 000 - 004 002
CH1P K, G, S F81 CH1 PARITY FOR SIMPLE PROCEDURE 000 - 001 000
TRANSMISSION
CH1S K, G, S F82 CH1 STOP BIT FOR SIMPLE PROCEDURE 000 - 001 001
TRANSMISSION
CH1L K, G, S F83 CH1 SIMPLE PROCEDURE/LEVEL 2 000 - 001 001
TRANSMISSION
CH1M K, G, S F84 TRANSMISSION BY SIMPLE PROCEDURE 000 - 001 001
NORMAL/AOC-17 COMPATIBLE
CH2B K, G, S F85 CH2 BAUD RATE 000 - 004 000
ATSP K, G, S F90 AUTOMATIC STOP FUNCTION OFF/ON 000 - 001 001
ARSG K, G, S F91 READY SIGNAL POLARITY OPEN/CLOSE 000 - 001 000
SORD K, G, S F92 AOC SINGLE/DUAL 000 - 001 000
TLET K, G, S F93 RACK SHORT/LONG 000 - 001 000
TSEL K, G, S F94 RACK POSITION WHEN AUTOSAMPLER IS 000 - 002 001
USED
DUAL K, G, S F95 MAIN-AOC/SUB-AOC 000 - 001 000
TLT2 K, G, S F96 RACK FOR AOC-17/FOR AOC-20i 000 - 001 001
RDYF M MONITOR WHETHER ANALYSIS IS BEING 000 - 001
PERFORMED
NINJ M MONITOR INJECTION NUMBER 000 - 099
NSPS M MONITOR SAMPLE WASH NUMBER 000 - 099
NSLW M MONITOR SOLVENT WASH NUMBER 000 - 099
NPMP M MONITOR PUM FREQUENCY 000 - 099
NTIM M MONITOR ANALYSIS (START) TIME 000 - 999
ISNO M MONITOR SAMPLE No. 000 - 150
ISN2 M MONITOR SAMPLE No. OF SUB-AOC 000 - 150
STRF M MONITOR START STATUS 000 - 001

4 External Control
4.3 Example Programs

Example programs for printing Sample Number and Injection number on a C-R7A Chromatopac are given
below, along with an explanation of the programs.
"4.3.1 Example Program for Simple Procedure Transmission" P.142 shows Simple procedure example,
and "4.3.2 Example Program for Level 2 Transmission" P.144 shows a Level 2 transmission example.
4.3.1 Example Program for Simple Procedure Transmission

List 4-1
100 PO=3
110 A$="MISNO" +CHR$(0DH)
120 B$="MNINJ" +CHR$(0DH)
130 S$="KSTRT" +CHR$(0DH)
140 OPEN TRS PO : CT=0
150 PRINT #PO,S$; : GOSUB 340
160 IF ((AS$="NK" OR AS$="") AND CT<4) THEN CT=CT+1 : GOTO 150
170 IF CT>=4 THEN LPRINT "ERROR" : CLOSE TRS PO : GOTO 420
180 CLOSE TRS PO
190 WAIT START
200 OPEN TRS PO : CT=0
210 PRINT #PO,A$; : GOSUB 340
220 IF ((AS$="NK" OR AS$="") AND CT<4) THEN CT=CT+1 : GOTO 210
240 SNS=AS$ : CT=0
250 PRINT #PO,B$; :GOSUB 340
260 IF ((AS$="NK" OR AS$"") AND CT<4) THEN CT=CT+1 : GOTO 250
280 NI$=AS$
290 CLOSE TRS PO
300 IF SN$="1*" THEN SN$="100"
305 LPRINT : LPRINT
310 LPRINT "SAMPLE No. : ";SN$;" REPEAT : ";NI$"
315 LPRINT
320 WAIT STOP
330 GOTO 190
340 REM *INPUT SUBROUTINE
350 T=TIME : WT=30 : AS$="" : AB$=""
360 IF T+WT<TIME THEN GOTO 410
370 IF (TRSF%(PO) AND 01H)<>0H THEN AB$=INPUT$(1.PO)
380 IF AB$=CHR$(0DH) THEN GOTO 410
390 IF AB$=<>"" THEN AS$ [LEN(AS$)+1]=AB$
400 AB$="" : GOTO 360
410 RETURN
420 END

4.3 Example Programs
Program explanation
Line Number Explanation

100 Defines the port used by the Chromatopac RS-232C interface. When a port other than No. 3 is
used, use that number on this line.
110 - 130 These lines define the commands to be sent to the autoinjector. A$ displays the Sample No., B$
displays the Injection No., and S$ STARTs operation.
140 - 180 This starts the autoinjector operation. If "NK CR " is returned in response to the command, or if
no response is returned, the command transmission is repeated up to 4 times. If a normal
response is not returned on the fourth transmission, "ERROR" is printed and program stops.
(Repeat execution of the program until normal transmission is established.)
It is necessary to include the semi-colon (;) in line 150, which contains the statement to send the
command. Transmitting "PRINT #PO,S$" without the ";" will cause" CR LF " to be appended to
S$ in the transmission.
190 This causes the program to wait until the Chromatopac analysis starts (sample injection is
performed). When the autoinjector completes the injection, the Sample No. and Injection No. will
be displayed on the CRT monitor.
This causes the Sample No. to be displayed during analysis. 4
200 - 240 The Sample No. is displayed, substituting the value for SN$.
250 - 290 The Injection No. is displayed, substituting the value for NI$.
300 When F84 is 1, when the Sample No. is 100, this indicates that the "1*" response should be
corrected to "100".
310 This prints out the Sample No. and Injection No.
320 This causes the program to wait until the Chromatopac analysis stops (the time set using the
STOP TM key).
340 - 410 This is a sub-routine for input of responses from the autoinjector. Each time a character is
received, it is input; this routine ends when a " CR " is received. The routine will also end when
reception continues for about 30 seconds, thereby exiting from the routine after a fixed period of
time if a response is not received after a command is transmitted.
When the characters "INPUT #" are used, the reception routine will end after 1 line is received,
and in this case, the program execution will stop if the response from the autoinjector is not
received normally.
Operation procedure
1 Set the parameters for the autoinjector, GC and C-R7A.

Set the program time of the gas chromatograph longer than the data processor analysis time. (For
Chromatopac, add the printout time to STOP.TIME.)
2 Execute (RUN) the program.

When the START command is sent from the C-R7A, the autoinjector will start operation.
3 The sample will be injected.

When the C-R7A starts analysis, the Sample No. and Injection No. are transmitted for display on the
monitor and then printed out. This sequence is then repeated for each analysis.

4 External Control
4.3.2 Example Program for Level 2 Transmission

List 4-2
100 OPEN TRS 3

110 STRT#3
120 WAIT START
130 SN$=ISNO#3M
140 NI$=NINJ#3M
150 LPRINT:LPRINT
160 LPRINT"SAMPLE No.:";SN$;" REPEAT :";NI$
170 LPRINT
180 WAIT STOP
190 GOTO 120
200 END
Program explanation
Line Number Explanation

110 This starts the autoinjector operation.
120 This causes the program to wait until the Chromatopac analysis starts.
130 - 160 This prints out the Sample No, and Injection No.
180 This causes the program to wait until the Chromatopac analysis stops.
190 Repeat lines 120 - 180.

5 5 Maintenance
@ NOTE
Clean the cover of the unit with a soft cloth dampened with water or a solution of water and a mild detergent. 5
5.1 Cleaning the Vials 5
Sample and solvent vials can be re-used if they are cleaned after each use. Follow the procedure below to
ensure that vials are as clean as possible. 5
1 Remove and discard the caps and septa.
5
5
2 Place the glass part of the vials in a flask made of material that can tolerate a vacuum,
and add the cleaning solution (descaler, organic solvent, water etc.).
5
3 Reduce the pressure in the flask, with an aspirator etc., to extract the air from the vials.
5
4 Gradually allow the pressure to return to normal. The vials will fill with cleaning
solution.
5
5 Place the flask in an ultrasonic cleaning device.
5
6 Discard the cleaning solution, and wash with water.
If acid solution was used, rinse with water at least three times using the procedure described above. 5
7 If water remains, dry the vials with acetone, alcohol or another highly volatile solvent.
5
8 Use new septa on clean vials.
5
5
5
5
5
5 Maintenance
5.2 Syringe Inspection and Maintenance

For details on the inspection and maintenance procedure, see "7.1 Precautions on Use of the
Microsyringe" P.155 and "7.2 Maintenance of the Microsyringe" P.156.
! CAUTION
• Since a syringe is manufactured precisely, always use only the plunger supplied with the
syringe. Plungers are not interchangeable.

5.3 Consumable Parts and Standard Accessories
5.3 Consumable Parts and Standard Accessories

5.3.1 Autoinjector Consumable Parts
Syringe (10 μl) S221-34618
Syringe (50 μl) S221-45243
Syringe (250 μl) S221-45244
Syringe (0.5 μl) 000445 By Shimadzu GLC
Syringe (5 μl) 5F-S-0.63 By Shimadzu GLC
Large vial (4 ml) S221-34267-92 50 pcs.
Large cap (4 ml) S221-34268-92 50 pcs. (white)
Large septum (4 ml) S221-34266-92 50 pcs.
Small vial (1.5 ml) S221-34272-92 100 pcs.
Small cap (1.5 ml) S221-34273-92 100 pcs. (white) 5
Small septum (1.5 ml) S221-41239-91 100 pcs.
Plunger holder S221-45177-91 5 pcs.
Small vial set S221-34274-91 1.5 ml vial, cap and septum, 100 pcs.
Large vial set S221-34269-91 4 ml vial, cap and septum, 50 pcs.
Barrel holder S221-45178-91 5 pcs.
Super elastic microsyringe (10 μl) S221-49548 Shimadzu GLC Part No.: MS-E10AOC23G
Super elastic microsyringe (5 μl) MS-E05AOC23G By Shimadzu GLC
@ NOTE
The table above only lists the main syringes with 23 gauge needles.
5.3.2 Autoinjector/Autosampler Accessories

In addition to the consumables listed in "5.3.1 Autoinjector Consumable Parts" P.147, the autoinjector/
autosampler has various accessories described in "1.5 Accessories List" P.6. To purchase required parts,
please see the consumable list in "1.5 Accessories List" P.6 and make an order.

5 Maintenance
5.4 Maintenance Parts

Unit Name Specification Parts No.
AC100 - 120 V Fuse 250 V 5 A TYPE T S072-02004-23
AC220 - 240 V Fuse 250 V 3.15 A TYPE T S072-02004-21
@ NOTE
The design life of this instrument is seven years. After being installed for seven years, the instrument's
maintenance parts will need to be inspected.
! WARNING
For continued fire protection, replace only with fuses of the same type and rating.

5.5 Optional Parts
5.5 Optional Parts

Optional autoinjector parts

Long rack assembly S221-45622-91 Sample capacity: 12
Small sample cooling fan assembly S221-44995-91
FPD fan assembly S221-44996-91 Required for the GC-14A/B, 15A,16A when FPD is
used
50 μl syringe S221-45243
250 μl syringe S221-45244
Syringe for OCI (10 μl) S221-37282-02 Required when OCI INJ is used
Optional autosampler parts
Name Part No. Remarks 5

4 ml vial rack S221-44878-91 See "2.7.3 Using 4 ml Vials (Option)" P.70
1.5 ml vial cooling/heat insulating rack S221-44998-91 Requires constant temperature circulating water
tank separately.
4 ml vial cooling/heat insulating rack S221-44999-91 Requires constant temperature circulating water
tank separately.
Optional C-R7A parts

Fiber optic/RS-232C transmission S223-02983-91
interface
Fiber optic cable S070-92025-52 2m
Optional parts for GC-2014

AOC-20i Installation kit S221-48545-92 Used for mounting the existing AOC-20i (external
power type) to GC-2014
AOC-20i Installation parts S221-44548-94 Used for attaching the AOC mounting guides on
multiple injection ports beforehand

5 Maintenance
Optional parts for GC-2010/2010Plus

AOC-20i Installation kit S221-48545-91 Used for mounting the existing AOC-20i (external
power type) to GC-2010/2010Plus
AOC-20i Installation parts S221-44548-93 Used for attaching the AOC mounting guides on
multiple injection ports beforehand

6 6 Troubleshooting
6.1 Error Codes 6

6.1.1 Autoinjector Error Codes
If the autoinjector does not operate normally, an error code is displayed. The various error codes are listed
6
and described below.
Table 6-1 Error codes and troubleshooting 6
Error code Troubleshooting
-01 Rack not in home position or not operating properly
• Rack has not moved completely to home position.
6
↓
Remove the rack. Clean the rack gears and gear wheel thoroughly. Place the rack again
and press the [RESET] key.
• Rack not in place.
6
↓
-02
Install the rack and press the [RESET] key.
Syringe not in home position or not operating properly
6
• Syringe not completely in home position.
↓
Press the [RESET] key. 6
-03 Plunger not in home position or not operating properly
6
• Plunger not completely in home position.
↓
Press the [RESET] key.
• Plunger does not move smoothly. (See Note 1.)
↓
Turn the power off, remove the syringe and perform maintenance on or replace the plunger. 6
-04 Autoinjector is not READY to start.
• The [START] key was pressed after the autoinjector experienced an error.
↓
Press the [RESET] key to clear the error and then, press the [START] key.
6
6
-05 Autoinjector memory error (RAM) and values initialized. (See Note 2.)
↓
Re-enter the parameters.
6
-06 Autoinjector memory error (ROM) (See Note 3.)
-08 CH2 error (transmission for sub-injector or the autosampler.)
↓
6
(For GC-2010/2010Plus/2014/2025, check if the analytical line setting is correctly configured.)
-09 No PRIORITY sample (set by transmission - see Note 4) or no sample in the position specified
by the INJECTION SAMPLE.
↓
Press the [RESET] key to restart after the sample has been placed in the rack.
6
-10 Memory (RAM) does not operate correctly. (See Note 3.)
-11 Autoinjector not correctly installed
↓
6
Re-install the autoinjector.
6
6 Troubleshooting

-12 The CH1 transmission line connecting the computer or the Chromatopac is not operating
correctly. (See Note 3.)
-13 The CH2 transmission line connecting the sub-injector or the autosampler is not operating
correctly. (See Note 3.)
-14 The waste liquid vial is not in position.
↓
Place the vial in position. Press the [RESET] key to restart.
Note 1) If the plunger movement becomes slightly stiff, no error is generated, but a warning is displayed (the LED to the left
of the [SAMPLE SIZE] key blinks while waiting for analysis) and operation continues.
Note 2) The error code "-05" is displayed when a RAM error occurs, and also when parameters are initialized. If this error
occurs every time power is supplied, the RAM back-up battery may be dead. For battery replacement, contact your
Shimadzu representative.
Note 3) When errors "-06" or "-10" occur, the autoinjector does not operate. When error "-12" occurs, the autoinjector
cannot be controlled externally. When error "-13" occurs, the sub-injector or autosampler cannot be used. If these
errors occur, contact your Shimadzu representative.
Note 4) When a PRIORITY SAMPLE is set up by the keypad, no error is generated when there is no sample in the
position; the next sample number is injected. An error is generated only when a PRIORITY SAMPLE has been set
up by transmission from an external device, and there is no vial in that position. When an INJECTION SAMPLE is
set up, whether by keypad or transmission, an error is generated if no vial is found in the position specified.

6.1 Error Codes
6.1.2 Autosampler Error Codes

When the autosampler generates an error, the error codes are displayed on the autoinjector. (For dual
AOC, these error codes are displayed on the main injector.)
Table 6-2 Autosampler error codes and troubleshooting

E01 Arm does not rotate properly.
↓
E02 Arm does not move properly along the x- or y- axis.
↓
E03 Arm does not move up and down properly.
↓
E04 Autosampler is not READY to start.
• The [START] key was pressed after the autosampler experienced an error.
↓
Press the [RESET] key to clear the error, then press the [START] key.
E05 An error occurred when placing the vial in the rack.
• A vial is already in position where the vial is to be placed.
↓
Remove the extra vial and press the [RESET] key.
• Vial cannot be placed in the rack.
↓
6
Check the rack. Replace the vial in the autosampler, and press the [RESET] key, or perform
teaching process.
E06 An error occurred when returning a vial to the autosampler.
• A vial is already in position where the vial is to be placed.
↓
Remove the extra vial and press the [RESET] key.
E07 An error occurred when holding/releasing a vial.
• The grip motor did not operate normally.
↓
Replace the vial and press the [RESET] key.
• The vial could not be held or released.
↓
Check and replace the vial and press the [RESET] key.
• The grip did not let go of the vial when the [RESET] key was pressed.
↓
Remove the vial from the grip and press the [RESET] key again.
E08 Vial was removed during vial transfer.
↓
Replace the vial and press the [RESET] key.
E09 No vial in the position specified by PRIORITY SAMPLE. (See Note 1.)
No vial in the position specified by INJECTION SAMPLE.
↓
Place the vial in position and press the [RESET] key.
Note 1) This error is generated only when the priority sample setting was entered by transmission (through an external
device) before start.
When it has been set up after start, an error is generated whether the priority sample was set up by the keypad or
by transmission.

6 Troubleshooting
6.1.3 Sub-injector Error Codes

When a sub-injector error occur in dual injector system, its display shows an error code. On the main
injector, the error code is displayed with a dash after, rather than before, the error code, as in "01-". If an
"E05" error occurs on the sub-injector, then "05E" is displayed on the main injector.
6.1.4 Miscellaneous Error Messages

Message Troubleshooting
OP The door is open. (Opening the door during operation causes the autoinjector to STOP.)
↓
Close the door. (The display returns to normal.) Press the [START] key to restart.
LE1 A CH1 transmission line error has occurred (this transmission line connects the computer or
Chromatopac).
↓
Redo the cable connections, and re-type the OPEN command for the autoinjector port.
LE2 A CH2 transmission line error has occurred (this transmission line connects the sub-injector or
autosampler).
↓
Redo the cable connections, and press the [RESET] key.
(For GC-2010/2010Plus, verify that the analytical line has been properly set.)

To Achieve Good
7 7
Analysis Reproducibility
Please observe the following precautions when using the AOC-20i to ensure reliable analysis results.
@ NOTE 7
The microsyringe (P/N S221-34618) used in the autoinjector is specifically designed for the AOC. Be sure to
7
use the specified microsyringe.
7.1 Precautions on Use of the Microsyringe 7

The life and the analytical reproducibility of the microsyringe will vary considerably depending on how well
it is maintained. Observe the following precautions in daily use of the microsyringe.
• After use, be sure to wash the microsyringe thoroughly with solvent, etc. Do not leave the microsyringe with
7
traces of sample, etc. remaining on the end of the needle, inside the barrel or in the plunger.
• Before turning on the power of the autoinjector, always test that the plunger moves up and down smoothly by
moving the plunger holder by hand. If the plunger does not move smoothly, remove the microsyringe from 7
the autoinjector, and perform maintenance of the microsyringe. (For the maintenance procedure, see "7.2
Maintenance of the Microsyringe" P.156.)
• After replacing the injection port septum, turn off the power of the autoinjector and move the syringe drive 7
unit up and down to make a hole in the injection port septum once, then turn on the autoinjector. This is to
7
ensure that an excessive force will not be applied at the end of the needle, which would reduce the life of the
needle.
• Do not operate the plunger of the microsyringe when solvent or sample is not supplied sufficiently. This may
cause the barrel glass and the plunger of the autoinjector to seize up against each other and be damaged.
• Before starting to use the autoinjector, check that fragments of the septum, etc. are not clogged at the end of
the syringe needle. (In the first operation of a sequence using the autoinjector, check that the microsyringe is
7
smoothly filled with solvent or sample.)
7
! CAUTION
• The microsyringe is manufactured with extremely high precision. Always use the syringe
main unit and the plunger in the original combination. It is not permitted to replace only the
7
plunger and use the original syringe and a new plunger together.
For attachment and removal of the microsyringe, see "2.2 Mounting and Removing the Syringe" P.26.
7
7
After attaching the microsyringe and fixing the plunger, check that the plunger is touching the lower position
of the syringe barrel.
7
7
7
7
7.2 Maintenance of the Microsyringe

When performing maintenance of the microsyringe, remove it from the autoinjector.
(1) Maintenance of the plunger

Repeatedly aspirate and discharge solvent, etc. into/from the micro syringe, and check whether the
plunger is moving smoothly. Move the plunger a full stroke, and check whether the plunger moves
easily without being caught or other abnormalities.
If the plunger of the syringe does not move smoothly, the reproducibility may deteriorate, so be sure
to perform maintenance.
Wash the syringe and the plunger with solvent, etc. and perform pumping to remove dirt, etc. from
the inside of the syringe barrel and the plunger. A soft cloth, moistened with solvent etc., can be
used effectively to wipe dirt off the plunger after removing it from the barrel. (Black marks on the
plunger are caused by stainless powder.) Wipe the plunger thoroughly, set it into the syringe barrel,
then perform pumping with solvent to confirm that it moves smoothly.
If the plunger does not move smoothly after several series of pumpings, washing or wiping during
maintenance was insufficient. In these cases, wash and wipe the syringe and the plunger again in
the same way, and check that the syringe moves smoothly, then attach the syringe to the
autoinjector.
If the syringe is very dirty, it may be more effective to pull the plunger out of the syringe barrel and
wash both the plunger and the barrel with ultrasonic waves in cleaning detergent or solvent (for
approximately 1 min.).
Further, when analyzing water solution samples, use a super elastic microsyringe (See "5.3.1
Autoinjector Consumable Parts" P.147.) to prevent the plunger movement from becoming
constricted.

7.2 Maintenance of the Microsyringe
(2) Maintenance of the end of the syringe needle

Repeatedly aspirate and discharge solvent, etc. into/from the microsyringe, and check how the
sample is discharged from the end of the needle. Under normal conditions, the sample is
discharged in a straight line from the end of the needle. However, if the end of the needle is clogged,
the sample may be dispersed as a spray or discharged at an angle. Or even if the sample is
discharged in a straight line, the discharge diameter may be smaller than under normal conditions.
(To make the comparison easier, it is recommended that you compare the discharge from the
existing needle with that from a new needle that does not have any clogging.)
If the sample is discharged abnormally, the sample vaporization characteristics inside the glass
insert may change at every injection and the analytical reproducibility may deteriorate. Maintenance
of the end of the needle is required to prevent such abnormalities from occurring.
Remove clogging from the end of the needle by using a syringe cleaning wire (5 wires in 1 set,
Shimadzu GLC), and check that the sample is discharged normally before using the syringe.
To remove dirt effectively from the inside of the syringe and the end of the needle, aspirate dirt using
an aspirator or the syringe cleaner.
Normal Maintenance is required.
Figure 7-1

7.3 Precautions on Packing with Silica Wool

(1) Amount of silica wool
Although the appropriate amount of silica wool may vary slightly depending on the sample being
analyzed, use the following amount as a standard guide.
• Split analysis: Approx. 10 mg
• Splitless analysis: Approx. 2 mg (Approx. 1 mg in the GC-17A)
However, for a sample with high absorptivity such as agricultural chemicals, a good analytical result
may be obtained when the silica wool amount is decreased. On the other hand, for samples with
large vaporization latent heat such as water, a better analytical result may be obtained when the
silica wool amount is increased.
@ NOTE
The silica wool offered as standard accessory with the GC-2010/2010Plus/2014/2025 (SPL) is different from
conventional silica wool, and is inert. This silica wool has thick fibers and low density. Accordingly, even if the
weight is the same, its volume is greater than that of conventional silica wool.
(2) Stuffing position of silica wool

The silica wool should be packed in the glass insert in a position that is at a distance of 1 to 2 mm
from the end of the needle during injection.
If the needle is too far from the silica wool or if the needle touches the silica wool, the peak shape
and the reproducibility of the analysis results may deteriorate. See the figure below when packing
the silica wool.

7.3 Precautions on Packing with Silica Wool
@ NOTE
For standard GC-2014/2025 split analysis, silica wool is packed at 20 mm from the insert end. In this case,
however, the needle tip is inserted approximately 5 mm into the silica wool. This is for obtaining stable
reproducibility regardless of how samples are discharged from the needle tip. If you wish to further improve
reproducibility, place the silica wool at 25 mm from the end, as in the case of GC-2010/2010Plus.
GC-2010/2010Plus/2014/2025
Splitless and GC-14B: 25 mm
GC-17A and GC-1700: 21 mm
GC-2014/2025 Split: 20 mm
2 mg∗1 5 - 6 mm
10 - 15 mm 10 mg
∗1 Because the inner diameter is

small in the glass insert offered
Silica wool as a standard accessory of the
(Pack as evenly as possible GC-17A and the GC-1700, the
while taking care that the top proper amount is approximately
end does not become fluffy.) 1 mg.
For split analysis For splitless analysis/WBI direct injection.

7
(In the glass insert offered as a standard
accessory of the GC-17A and the GC-1700,
the lower portion is not narrower.)
Figure 7-2
@ NOTE
For splitless analysis in which the flow velocity inside the insert is slow, and WBI direct injection in
which the inlet pressure is low, it may be better to pack the silica wool at a lower position. This is due
to INJ port pressure fluctuations during vaporization and the distance from the silica wool to the
insert top end. The optimal amount of silica wool and its position also vary depending on the type of
solvent, the temperature in the injection port, and the volume of the sample.

7.4 Precautions on Replacing the Septum

Replace the injection port septum after a fixed number of injections so that fragments of the septum do not
clog the syringe. (It is recommended to replace the septum after every 100 injections.)
When replacing the septum, do not tighten the septum nut too tightly. Close the septum nut by hand until it
stops, then return it by a half turn. If the septum nut is tightened too tightly, the injection port septum will be
compressed more than necessary. As a result, fragments of the septum can easily become clogged in the
syringe, and its durability will deteriorate.
For details on the septum replacement procedure, refer to the instruction manual of the gas chromatograph
and the GC maintenance help.

7.5 Other Precautions on Use
7.5 Other Precautions on Use

(1) Check that no gas leak is detected in the column connection areas and the tubing connection areas.
(2) In order to improve the reproducibility, it is advisable to replace the silicon septum used in solvent
vials and waste liquid vials with PTFE sheeting. By doing so, the chances of fragments of the
septum becoming clogged in the syringe are reduced.
For the same reason, it is also advisable to reduce the number of times for sample washing to 0 or 1
when analyzing multiple times using the same sample.
When using the GC-2010/2010Plus/2014/2025 (SPL), be sure to also observe the following
precautions.
(3) Be sure to attach the INJ/DET cover during analysis.
(4) When attaching the column, be sure to use the small nut (column nut for injection port) on the INJ
side.


Index
A B
AC power inlet .....................................................18 Bar code reader ................................................. 96
Accessories ...........................................................6 Barrel .................................................................. 27
autoinjector .....................................................6 Barrel holder ............................................... 27, 147
autosampler ..................................................11
Air suction ...........................................................95
Alloc ....................................................................96 C
Analysis (precautions) .......................................126
Cap (vial) .............................................. 47, 48, 147
Analysis start time ...............................................95
Case ................................................................... 15
Analysis time .......................................................95
CBM-101/102 ................................................. 4, 75
AOC cable ...........................................................21
CLASS-5000 ...................................................... 76
AOC other parameters ......................................102
CLASS-GC10 ..................................... 75, 110, 130
AOC parameters ...............................................101
CLASS-VP ....................................................... 132
AOC-20i installation kit ..............................149, 150
Cleaning ........................................................... 145
AOC-20i installation parts .........................149, 150
after injection ................................................ 87
Arm (autosampler) ..................................16, 64, 72 before injection ............................................. 86
Arrow keys ....................................................82, 89 Components ......................................................... 2
Aspirating air (syringe) ................................95, 116 Configuration
Aspiration amount system ........................................................... 3
pumping ........................................................95 Configure the instrument and peripherals ........ 105
sample wash .................................................95
Consumable parts ............................................ 147
Aspiration volume
Cooling/heating .......................................... 14, 149
solvent flush mode ........................................95
Autoinjector
accessories .....................................................6 D
error codes ..................................................151
installation parts ..............................................8
DEWELL TIME ................................................... 93
installing ........................................................21
Discrimination ..................................... 96, 113, 116
names ...........................................................15
part No. ...........................................................2 Display ................................................... 15, 82, 89
specifications ................................................13 Door ................................................................... 15
washing with 3 different solvents ....................45 Dwell time ........................................................... 93
Autosampler
4 ml vial rack .................................................70
accessories ...................................................11 E
arm ...................................................16, 64, 72
autosampler tray ..........................................118 ENTER key .................................................. 82, 91
error codes ..................................................153 Equilibration time .............................................. 107
installation parts (for GC-2014, GC-14A/B) .....11 Error codes ....................................................... 151
installing ........................................................64 autoinjector ................................................ 151
name ............................................................16 autosampler ............................................... 153
part No. ...........................................................2 sub-injector ................................................ 154
specifications ................................................14
Error messages ................................................ 154
Autosampler control terminal ........................17, 18
External control ................................................ 133
Autosampler tray ...............................................118 command ................................................... 140
Aux control terminal ............................................18

Index
F I
F21 ..................................................................... 95 Initializes the RAM ............................................. 97
Fiber optic cable ............................................... 149 INJ port septum .................................................. 57
Fiber optic link terminal ................................ 17, 18 INJ SPEED-PLUNGER ...................................... 93
Final priority sample No. .................................... 94 INJ SPEED-SYRINGE ....................................... 93
FINAL SAMPLE ......................................... 94, 112 Inj. speed (plunger) ............................................ 93
Flange ................................................................ 27 Injection
FPD fan ...................................................... 62, 149 mode ............................................ 93, 104, 113
FUNCTION key ............................................ 82, 92 sample ......................................................... 86
speed ........................................................... 93
Fuse ........................................................... 18, 148
INJECTION MODE ............................................ 93
Fuse holder ........................................................ 18
Injection port septum .......................................... 59
Injection procedure ............................................. 85
G INJECTION SAMPLE ................................. 94, 112
INJECTION SAMPLE2 ....................................... 94
GC network ................................................ 77, 110 Injection volume ................................................. 85
GC14/2014 Injector 1 control terminal ............................. 17, 18
mounting bracket .......................................... 73
Injector 2 control terminal ............................. 17, 18
GC-14A/B
Installation parts
mounting bracket .......................................... 11
autoinjector .................................................... 8
spacers ........................................................ 25
autosampler ................................................. 11
GC-2010 GC-14A/B, 15A, and 16A .............................. 10
AOC other parameters ................................ 102 GC-17A, 18A, and 1700 .................................. 9
AOC parameters ........................................ 101 GC-2010 ........................................................ 8
GC-2010Plus GC-2010Plus .................................................. 8
AOC other parameters ................................ 102 GC-2014 ........................................................ 8
AOC parameters ........................................ 101 GC-2014, GC-14A/B ..................................... 11
GC-2014 GC-2025 ........................................................ 9
AOC other parameters ................................ 102 Installing
AOC parameters ........................................ 101 autoinjector .................................................. 21
mounting bracket .......................................... 11 autosampler ................................................. 64
spacers ........................................................ 23 Internal standard ........................................ 68, 125
GC-2025 Isothermal analysis .......................................... 108
AOC other parameters ................................ 102
AOC parameters ........................................ 101
GCMS-QP5000 .................................................. 76 K
GCMSsolution .................................................... 75
GCsolution ......................................... 75, 110, 128 Keyboard unit ..................................................... 15
Gripper (autosampler) .......................... 16, 72, 122 Keypad ............................................................... 81
Gripping position ................................................ 70 Kinds of vials ...................................................... 95
Ground terminal ................................................. 18
H
Heating ............................................................... 14
Home position .............................................. 80, 87
Humidity ............................................................. 19

Index
L O
Large volume injection mode ............................115 OCI syringe ................................................ 35, 149
Light Operation environment ....................................... 19
power ..............................................16, 18, 122 OPTION ............................................................. 80
run ................................................................82 Option ........................................................... 62, 70
Line .....................................................................80 autosampler ................................................. 70
Loading Optional ................................................ 64, 73, 149
rack ...............................................................41 autoinjector ................................................ 149
Long rack ..............................................39, 62, 149 autosampler ......................................... 64, 149
Low-speed injection method .............................115 cooling/heating unit ....................................... 14
C-R7A ........................................................ 149
GC-2010 .................................................... 150
M GC-2010Plus .............................................. 150
GC-2014 .............................................. 73, 149
Maintenance ..............................................145, 156 long rack guide ............................................. 39
end of the syringe needle .............................157
plunger ........................................................156
syringe ........................................................156 P
vials ............................................................145
Maintenance parts .............................................148 Parameter
precautions .................................................. 88
Mode (injection) ...................................................93
Parameter keys ............................................ 82, 89
MONITOR key ...............................................82, 90
Parameters ......................................................... 83
Mounting
list .............................................................. 101
syringe ..........................................................26
setting .......................................................... 83
Mounting bracket
Plunger
GC14/2014 ...................................................73
maintenance ............................................... 156
Multi-Inj. .......................................................95, 115 speed ..................................................... 93, 95
Plunger button .................................................... 31
N Plunger drive unit ......................................... 15, 28
Plunger holder ............................................ 31, 147
Name Plunger suction speed ........................................ 95
autoinjector ...................................................15 Power indicator .................................................. 18
autosampler ..................................................16 Power light ....................................................... 122
power supply .................................................17
Power ON ......................................................... 122
Needle guide
Power supply
autoinjector .............................................15, 29
[AOC Parameters] window ............................ 80
injection port ..................................................60
AOC power supply .......................................... 6
Number (sample) ................................................90 built-in power ................................................ 17
Number of injection .......................................85, 90 external power .......................................... 7, 18
NUMBER OF INJECTIONS ............85, 89, 90, 110 GC-2010 ........................................................ 6
Number of injections ...........................................95 GC-2010Plus .................................................. 6
Number of priority injections ................................94 GC-2014 ........................................................ 7
GC-2025 ........................................................ 7
name ............................................................ 17
part No. ...................................................... 2, 6
Power switch ...................................................... 18
Power/status indicator ........................................ 16
PRE SOLVENT WASH ...................................... 93
Pre solvent wash ................................................ 93

Index
Precautions S
analysis ...................................................... 126
other .......................................................... 161 Sample
septum ....................................................... 160 injection ........................................................ 86
silica wool .................................................. 158 number ........................................... 79, 90, 119
syringe ....................................................... 155
Sample aspiration
Pretreatment setup speed ........................................................... 95
GC network ................................................ 132 waiting time .................................................. 93
Priority ................................................................ 94 Sample cooling fan ..................................... 62, 149
Priority analysis SAMPLE SIZE ........................................ 85, 89, 90
autoinjector ................................................ 111
Sample size .................................................. 85, 90
autosampler ............................................... 123
SAMPLE WASH ..................................... 85, 89, 90
PRIORITY SAMPLE ........................... 94, 111, 123
Sample wash ................................................ 85, 90
Priority sample No. ............................................. 94
aspiration amount ......................................... 95
PTV .................................................................. 115 speed ........................................................... 95
PUMPING .......................................................... 93 Septum ....................................................... 48, 147
Pumping ....................................................... 86, 93 INJ port ........................................................ 57
aspiration amount ......................................... 95 injection port ................................................. 59
speed ........................................................... 95 precautions ................................................ 160
vial ............................................................... 47
Septum nut ................................................... 21, 59
R
Sequential analyses ......................................... 104
Rack ............................................................. 38, 99 SET .................................................................... 80
loading ......................................................... 41 Set .................................................................... 147
long rack guide ............................................. 39 Settings
vial placement ........................................ 55, 79 parameters ................................................... 83
Rack gear ........................................................... 15 precautions .................................................. 88
Rack guide ......................................................... 15 Short rack guide ................................................. 38
Rack position ........................................ 70, 99, 117 Silica wool ........................................................ 158
READY signal polarity ........................................ 99 Solvent flush ....................................... 74, 113, 124
Removing aspiration volume ......................................... 95
autoinjector .................................................. 57 solvent ......................................................... 68
syringe ......................................................... 35 SOLVENT SELECT ........................................... 93
Reset .................................................................. 88 Solvent select ..................................................... 93
RESET key ................................................... 82, 91 Solvent vial (volume limit) ................................... 53
ROM version ...................................................... 97 SOLVENT WASH ................................... 85, 89, 90
RS-232C terminal ......................................... 17, 18 Solvent wash ................................................ 85, 90
Run light ............................................................. 82 Spacers
GC-14A/B ..................................................... 25
GC-2014 ...................................................... 23
Specifications ..................................................... 13
autoinjector .................................................. 13
autosampler ................................................. 14
Speed
injection ........................................................ 93
plunger ......................................................... 93
pumping ....................................................... 95
sample aspiration ......................................... 95
sample wash ................................................ 95
syringe ......................................................... 93
Speed of plunger ................................................ 95
START key ................................................... 83, 91

Index
START/READY terminal ...............................17, 18 V

Stop .....................................................................88
STOP key ......................................................83, 91 Vial ................................................................... 147
Suction volume for washing ................................95 cleaning ..................................................... 145
Super elastic microsyringe ................................147 loading in the rack ........................................ 79
sample volume ............................................. 48
Syringe (Microsyringe) ......................................147
septum replacement ..................................... 48
aspirate air ....................................................95
types ............................................................ 47
elastic .........................................................147
large volume ...............................................115 Vial detection ................................................ 88, 90
maintenance ...............................................156 Vial rack ..................................................... 16, 118
mounting .......................................................26 1.5 ml ..................................................... 38, 39
needle ...........................................................27 4 ml .................................................. 38, 39, 67
OCI ...............................................................35 for autosampler 1.5 ml .................................. 67
precautions .................................................155 for autosampler 4 ml ..................................... 67
removing .......................................................35 Vial racks ............................................................ 64
size ...............................................................95 VISCOSITY ........................................................ 93
speed ............................................................93 Viscosity ............................................................. 93
Syringe clip ..........................................................30
Syringe drive unit ..........................................15, 28
Syringe height (↑) ..........................................49, 95 W
Syringe height (inj) ..............................................95
Waiting time (sample injection) .......................... 93
Syringe height (↓) ..........................................50, 95
Washing times
System configuration .............................................3
after injection ................................................ 85
CLASS-GC10 ..............................................130
before injection ............................................. 85
GCsolution ..................................................128
Waste liquid vial (volume limit) ........................... 53
T
Temperature ........................................................19
Temperature programmed analysis ..................107
The position when returning ................................95
Transmission interface ......................................149
Tray .............................................................16, 118
Turning the power ON/OFF .................................80
U
Upload .................................................................81
Use of the sampler ..............................................96
Use of the sub AOC ............................................96
Use the same param ...........................................96
Using 3 solvent vials .....................................45, 95

Index

221-79041C AOC20i 20s IM EN

Uploaded by

Copyright:

Available Formats

221-79041C AOC20i 20s IM EN

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

221-79041C AOC20i 20s IM EN

Uploaded by

Copyright:

Available Formats

221-79041C

Read this Instruction Manual thoroughly before using the product.

Thank you for purchasing this product.

Keep this manual for future reference.

2012-2020 Shimadzu Corporation. All rights reserved.

Precaution symbols are indicated using the following conventions:

• Safety regulations and standards

• Prevent spills by never exceeding the waste liquid vial capacity.

 Inspection and Maintenance

AOC-20i/AOC-20i Plus/AOC-20s iii

Emergency Shutdown Operation

1. Turn OFF the switch on the power supply unit.

 During a Power Outage

 Power Supply Unit

Shimadzu provides the following warranty for this product.

Maintenance, Inspections, and Adjustment

AOC-20i/AOC-20i Plus/AOC-20s vii

Contact Shimadzu service representative when the equipment has reached

viii AOC-20i/AOC-20i Plus/AOC-20s

Descriptions of this section are only applied to the following models:

 EN61326-1 Emissions (Electromagnetic Interference)

 EN61326-1 Immunity (Electromagnetic Susceptibility)

When installing or using this product, especially in an industrial location:

To prevent static electricity:

Indications Used in This Manual............................................................................................. ii

Safety Instructions .................................................................................................................. ii

After-Sales Service and Availability of Replacement Parts .................................................. vii

Maintenance, Inspections, and Adjustment.......................................................................... vii

Disposal Precautions............................................................................................................ vii

Action for Environment (WEEE) ...........................................................................................viii

Electromagnetic Compatibility ............................................................................................... ix

1.2 Components ........................................................................................................2

1.3 System Configuration and Operation...................................................................3

1.6 Features ............................................................................................................ 12

1.7 Specifications .................................................................................................... 13

1.8 Part Names........................................................................................................ 15

1.9 Operation Environment...................................................................................... 19

2.2 Mounting and Removing the Syringe ................................................................ 26

2.4 Vial Types and Vial Mounting Procedure on Rack Guide.................................. 47

2.5 Replacing INJ Port Septum ............................................................................... 57

2.6 Available Options............................................................................................... 62

2.7 Installing the Autosampler (AOC-20s) [Optional]............................................... 64

xii AOC-20i/AOC-20i Plus/AOC-20s

2.7.5 Adjusting Sample Gripping Position (Teaching)............................................... 70

2.8 Controlling via Workstation ................................................................................75

3.2 Display and Keypad Functions ..........................................................................89

3.3 Sequential Analyses and Injection Modes .......................................................104

3.4 Autosampler Operation....................................................................................117

3.5 Analysis Tips and Precautions.........................................................................126

AOC-20i/AOC-20i Plus/AOC-20s xiii

3.6 GCsolution....................................................................................................... 128

3.7 CLASS-GC10 .................................................................................................. 130

3.8 CLASS-VP....................................................................................................... 132

4.2 External Control............................................................................................... 135

4.3 Example Programs .......................................................................................... 142

5.2 Syringe Inspection and Maintenance .............................................................. 146

5.3 Consumable Parts and Standard Accessories ................................................ 147

5.4 Maintenance Parts........................................................................................... 148

5.5 Optional Parts.................................................................................................. 149