B.sc. Microbiology 1

APPENDIX - AL
MADURAI KAMARAJ UNIVERSITY

(University with Potential for Excellence)
SYLLABUS FOR B.Sc. MICROBIOLOGY

CBCS SEMESTER PATTERN
(with effect from the academic year 2023-2024 onwards)
1. Introduction of the programme
This three year Bachelor of Science course in Microbiology deals with the study of
microorganisms comprising Bacteria, Fungi, Protozoans, Algae and Virus; and its association with
the environment, plants, animals and humans. Candidates undertaking this curriculum will
understand the basic and applied concepts of Microbiology. This includes the beneficial and
harmful role of microorganism in the production of commercially important products and its role in
various diseases respectively. Basic concepts of Immunology of the host and its interaction with
infectious microorganisms are also included in the syllabus. The scope of this course is wide
which enables the candidate to get placed in diagnostics, pharma, fermentation, dairy, food and
medical arena.
2. Eligibility for admission:
A candidate who has passed Higher Secondary examination (10+2) conducted by the Board of
Higher Secondary Education, Govt. of Tamil Nadu or any other examinations accepted as
Equivalent thereto by the syndicates subject to such conditions
 Biology/Physics/Chemistry as subjects in the Higher Secondary education.
 Candidates should have secured at least 60% in the above subjects individually and as
total aggregates
 A relaxation of 10% marks in the aggregate shall be given to SC/ST candidates
2.1 Duration of the programme:
The students will undergo the prescribed course of study for a period of not less than three
academic years (six semesters)
The maximum duration for completion of the UG Program shall not exceed twelve semesters,
beyond which the candidate has to get readmitted in the course with the new syllabus if any.
2.2 Medium of Instructions of the programme: English
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3. Objectives of the Programme
 To inculcate the basic and advanced concepts of Microbiology including taxonomy,

physiology, Immunology, biomolecule interactions, genomics, proteomics and rDNA
technology.
 To impart the scope for the application of concepts learned in the subject.
 To introduce about the recent advances in the field of Microbiology and its importance in
research.
4. Outcome of the programme:

At the end of this three year course, a candidate will have a thorough understanding on the basic
concepts of Microbiology and its applications in the various fields of science and technology.
Through the knowledge and hands-on experience imparted during the practical subjects, the
candidate will get conveniently placed in the diagnostics, production and R&D units of various
hospitals and industries respectively. This course will also lay a strong foundation to build the
individual research caliber in the aspirants of Bachelor of Science in Microbiology.
5. Core subject papers:
Title of the Paper Weekly No. of Exam Marks
Contact Credits Hours
Int. Ext. Tol.
Hours
Semester – 1
General Microbiology 4 4 3 25 75 100
Microbial Physiology & 4 4 3 25 75 100
Taxonomy
Major Practical -1 (Basic 2 0 0 0 0 0
Microbiology, Microbial
physiology and Biochemistry)
Semester – 1I
Biochemistry 4 4 3 25 75 100
Major Practical -1 (Basic 2 4 3 40 60 100
Microbiology, Microbial
physiology and Biochemistry)
Semester-III
Microbial Genetics & Molecular 4 4 3 25 75 100
Biology
Major Practical-II Microbial 2 0 0
genetics, Molecular Biology &
Industrial Microbiology)
Semester -IV
Industrial Microbiology 4 4 3 25 75 100
Major Practical – II Microbial 2 4 3 40 60 100
2
genetics, Molecular Biology, &
Industrial Microbiology
Semester V
Bioinformatics 5 4 3 25 75 100
Medical Microbiology 5 5 3 25 75 100
Soil & Agricultural 4 4 3 25 75 100
Microbiology
Major Practical – III 4 0 0
(Bioinformatics, Medical
Microbiology & Soil and
Agricultural Microbiology
Major Practical – IV 4 0 0
(Biotechnology & Immunology,)
Semester VI
Biotechnology 4 4 3 25 75 100
Immunology 4 4 3 25 75 100
Major Practical – III 4 4 3 40 60 100
(Bioinformatics, Medical
Microbiology & Soil and
Agricultural Microbiology
Major Practical – IV 4 4 3 40 60 100
(Biotechnology & Immunology,)
6a. Subject elective papers
rDNA Technology & Tissue 4 3 3 25 75 100

culture Technology (Theory &
Practical
6b. Skill based subjects
Mushroom Technology 2 2 3 25 75 100

BioControl 2 2 3 25 75 100
Medical Laboratory Technology 2 2 3 25 75 100
7. Non-Major Elective papers
Basic Microbiology 2 2 3 25 75 100

Food & Dairy Microbiology 2 2 3 25 75 100
8. Unitization: Content of every paper divided into FIVE units.
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9. Pattern of semester examination
Examinations will be conducted at the end of each semester. Each semester has two pattern of
examinations for theory paper, namely Internal (25) and External (75). Each semester has two
pattern of examinations for practical paper, namely Internal (40) and External (60).
10. Scheme of Internal Evaluation:
The pattern of Internal valuation:
Tests - 10 marks (Average of the best two tests)

Assignment - 5 marks
Seminar/Group Discussion - 5 marks
Peer-Team Teaching/Quiz - 5 marks for all subjects
Total - 25 marks
Core Practical Internal examination
The pattern for internal valuation for 40 marks may be

Two internal tests of 25 marks : Average = 25 marks
Observation Book : 10 Marks
One assignment : 05 Marks
11. External Exam : As per the information furnished in No.9.
Examinations
The duration of theory examination shall be three hours to each paper at the end of each semester.
The candidate failing in any subject(s) will be permitted to appear for each failed subject(s) in the
subsequent examinations. The practical examinations for UG course should be conducted at the
end of the academic year.
12. Question Paper Pattern
THEORY QUESTION PAPER PATTERN

Time: 3 hours Max. Marks: 75
Section- A (10 x 1 = 10 Marks) (Answer all the Question; 1 to 10)
1. Choose the correct answer type questions.

2. Two questions from each unit
3. Four choices with one correct answer in each question.
4. Answer all questions.
4
Section- B (5 x 7 =35 marks)
Answer all questions – Either Or type

Answers not exceeding two pages (One question from each unit)
Question Nos.
11a or 11b
12a or 12b
13a or 13b
14a or 14b
15a or 15b
Section - C (3 x 10 = 30 Marks)
Answer Any Three out of Five
Answer not exceeding four pages (One question from each UNIT) Question Nos. 16
- 20
CORE PRACTICAL QUESTION PAPER PATTERN

Time : 6 hours Maximum Marks (University Exam) - 60
Major Practical - 1 : 20 Marks

Minor Practical - 1 : 15 Marks
Spotters - 2 : 2 x 2.5 = 5 Marks
Record : 5 Marks
Viva voce : 15 Marks
Internal Marks : 40 Marks

Total : 100Marks
13. Scheme for evaluation

Section A – 10 X 1 = 10
Section B – 5 X 7 = 35
Section C – 3 X 10 = 30
Total = 75 (External)
14. Passing minimum
To get a pass, a student should fulfill the following conditions:
a) Theory:
i) Minimum 40% or above as total aggregated marks, including both internal and external.
ii) No separate minimum pass marks for the internal, however the candidate must secure a
minimum of 27 marks out of 75 in the external examination to be declared as pass.
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b) Practical
i) 40% of the aggregated (Internal +External)
minimum of 21 marks out of 60in the external examination to be declared as pass.
c) Project
i) 40% of the aggregated (Internal +External)
minimum of 28 marks out of 80in the external examination to be declared as pass.
Candidates who have secured 60% and above in aggregates of the Part III will be given First class;
Candidates who have secured 50% and above but less than 60% will be given Second class;
Candidates who have secured 40% and above but below 50% will be given a Third class.
Ranking will be made for the candidates who have necessarily completed the course without any
arrears in each semester and scored the maximum total among all candidates appeared for the
examination in the Part III will be given the First Rank. Such candidates will be honored with a
Gold Medal if there is a sponsorship or an endowment.
14.1. Classification
S.No Range of CCPA Class

1 40 & above but below 50 III
2 50 & above but below 60 II
3 60 & above I
15. Model questions

Section A
Answer all questions (10x1=10)
1. discovered Cellular immunity.

a. Robert Kotch b. Elie Metchnikoff c. Paul Ehrlich d. Karl Landsteiner
2. Administration of antigens is an example of immunity.

a. Non-specific b. Active c. Passive d. Memory
3. is an antibody which is abundantly present in breast milk.

a. IgG b. IgA c. IgE d. IgD
4. Two heavy chains of antibodies are joined together by .

a. Hinge region b. Fab c. Fc d. Light chain
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5. During allergic conditions which antibody concentration gets elevated?
a. IgG b. IgA c. IgE d. IgD
6. In Myasthenia Gravis, auto immunity is developed against .

a. Neurons b. Acetylcholine receptors c. Muscles d. Nephrons
7. Monoclonal antibodies are specific at the level of .

a. Antigen b. Epitope c. Fab d. Paratope
8. In Precipitation antigen is involved.

a. Soluble b. Particulate c. Both a&b d. Partial
9. ABO blood grouping was given by .

a. Robert Kotch b. Elie Metchnikoff c. Paul Ehrlich d. Karl Landsteiner
10. HIV targets cells.

a. CD4-Tcell b. CD8-T-cell c. B-Cells d. Neurons
Section B:
Answer all questions (5x7=35 marks)
11. a. Explain in detail about non-specific host defense mechanism with a few examples.
(or)
11. b. Elaborate on various cells of the immune system.
12. a. Explain classical complement pathway with its significance.
(or)
12. b. What are the different types of immunoglobulins? Write short notes on any three.
13. a. Differentiate hypersensitivity from autoimmunity with a few examples.
(or)
13. b. Write in detail about Type III hypersensitivity.
14. a. Explain double immunodiffusion using schematic representation and mention about
its types and principle.
(or)
14 b. Discuss about the production of monoclonal antibodies.
15.a. Briefly explain about Erythroblastosis fetalis.
(or)
15.b. How immunodeficiency is caused by HIV? Explain.
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Section C
Answer any three questions (3x10=30 marks)
16. What are the molecular tools available for rDNA technology? Discuss in detail
about their significances.
17. Explain Sanger’s method of sequencing with diagrammatic illustrations.
Explain the difference between Sanger’s method and Automated DNA
sequencing.
18. Describe different blotting techniques that are used in the rDNA technology
highlighting the principle behind each blotting techniques.
19. Discuss the different categories of risk groups and its biosafety guidelines.
20. Explain the microbes which live in extreme environments with suitable examples.
16. Teaching methodology

Each subject is designed with lectures / tutorials / seminar / Peer-Team teaching / PPT
presentation / Assignments, etc., to meet the effective teaching and the learning requirements.
10% of the course content must be taught through peer team teaching methodology.
17. Text books

1) Prescott, Harley and Klein. 2006, Microbiology 6/e. The McGraw-Hill Companies.
2) Pelczar, M.J.. Chan. E.C.S. and Kreig. N.R. 1993. Microbiology, Tata
McGraw-Hill Publishing Co. Ltd., New Delhi.
3) Schlegel. H.G. 1993. General Microbiology. Cambridge University Press,
Cambridge.
4) Stainer. R.Y., Ingraham, Wheelis, M.G. and Paintor. P.R.I986, The Microbial
World, Prentice Hall, New Jersey.
5) Tauro. P., Kapoor, K.K.and Yadav. K.S.I989, An Introduction to Microbiology,
Wiley Publications. New Delhi.
18. Reference books
1) Microbiology: A laboratory manual, P. Gunasekaran, New Age international
publishers, 1996.
2) Laboratory manual in general microbiology, N. Kannan, Panima publishers, 2002.
3) Microbiology: A laboratory manual. J.G. Cappuccino and N. Sherman, Additon-
Wesley, 2002.
4) Bergey's manual determinative bacteriology, J.G. Holt and N.R. Krieg. Lippincott
Williams & Wilkin publishers, 2000.
5) Moat AG. Foster JW and Spector MP. Microbial Physiology. 4/e Wiley-Liss,2002.
6) Caldwell DR. Wm. Microbial physiology and metabolism. C Brown publishers, USA
2002.
7) J.C. Cappuccino and N.Sherman, Microbiology: A laboratory manual, Addition –
Wesley, 2002.
8) M.T.Maigan, J.M. Martinko and J.Parkar, 2000. Brock Biology of Microorganisms,
(9th edition), Prentice- Jall.
9) C.J.Alexopoulos and C.W.Mims 1979, Introductory Mycology (3rd edition) Wiley,
9
New York.
10) L.W.Nester, C.N. Roberts and M.L.Nester 1995, Microbiology – A Human
Perspectives, Lowa, USA.
11) R.Y.Stainer, J.I.Ingraham, M.L. Wheelis and P.R.Painter 1999 General
Microbiological, McMillian Educational Ltd. London.
12) Principles of Biochemistry. Lehninger, AL. 1993 2nd edition, CSB Publishers.
13) Outlines of Biochemistry, 5/e - Conn. E.E., Stumpf, P.K. Bruening, G and Doi. R.H.
John Wiley & Sons (1987)
14) Biochemistry, Voet. D and Voet. JG. 1990. John Wiley & Sons. NY.
15) Text book of Biochemistry. 2/e. Devlin. T.M. 1986. Wiley Medical Publications, NY.
16) Biochemistry, 2/e, Stryer. L. 1998, W.H. Freeman and Company, NY.
17) Biochemistry, 2/e. Zubay. G. 1998. McMillan Publishers NY. Collier McMillan
Company Publishers, London.
18) Enzymes. Ribozymes and DNAzyrnes, P. Palanivelu, 2007, Twenty first Century
Publications, Palkalai Nagar, Madurai - 625 021.
19) Laboratory manual in biochemistry, 5/e, J. Jayaraman, New Age international
publishers, 1996.
20) Principles of practical biochemistry, K. Wilson and J. Walker, Cambridge University
press, 2000.
21) An Introduction to practical biochemistry, D.T. Plummer. TATA McGraw Hill, 1997.
22) Microbial Physiology, 4/e, Moat AG, Foster JW and Spector MP. Wiley-Liss. 2002.
23) Gene VII. Benjamin Lewin, 2000: Oxford University Press.
24) Molecular biology of the Gene,4/e. Watson, Hopkins, Roserts. Steits and Weiner, 1987,
The Benjamin/Cumming Publishing Company, Inc.
25) Molecular Genetics of Bacteria, 2/e, Larry Snyder and Wendy Champness, 2003, ASM
press. Washington DC.
26) Microbial genetics. David Friefelder, 1987, Narosa Publishing Mouse.
27) Essential ofimmunology, Roitt. I.M. 1998, ELBS, Blackwell scientific publication.
28) Immunology, 3/e. Kuby, J. 1997, W.H.Freeman and company. NY.
29) Crueger, W. and A. Crueger (2000), Biotechnology, A Text book of Industrial
Microbiology. Panima Publishers, New Delhi.
30) Flinger, M.C., and Drew, S.W., (1999), Encyclopedia of Bioprocess technology -
Fermentation, Biocatalysis and Bioseparation (Volumes I - V), John Wiley and Sons,
New York.
31) Sambrook, J. Cold Spring Harbor laboratory (2002).
32) Advanced bacterial genetics, David, RW, Botstein, D & Roth, JR. Cold
Spring
Harbor laboratory (1980).
33) Data basis in life sciences and Biotechnology: A directory - DBT, Govt. of India,
March 1995.
34) Protein Structure Analysis - Springer Lab Manual. R.M.Kamp, T.Choli-
Papadaopoulu B. WitmanLiebold.
35) Computer in microbiology- a practical approach. T.N, Bryant, JWT Wimpenny, IRL,
10
Press, 1989.
36) Jawetz. E. Melnic. JL. &Adelberg. EA. Medical microbiology 22/e McGraw Hill
Companies, 2004.
37) Rangasami G and Bagyaraj DJ. 1993. Agricultural Microbiology 2/e Prentice- Hall
publications
38) Rangasami G and Bagyaraj DJ. 1993. Agricultural Microbiology 2/e Prentice- Hall
publications.
39) Ronald Atlas, Bartha Richard, 1987. Microbial ecology 2/e Benjamin/ Cummings
publications.
40) Enzymes, Ribozymes and DNAzymes, P. Palanivelu, Twentyfirst
Century Publications. Palkalai Nagar, Madurai - 625 021 (2006).
41) Enzymes-Biochemistry, Biotechnology, Clinical chemistry- T. Palmer -East-
West press. New Delhi (2.004).
42) THEORY QUESTION PAPER PATTERN

Time: 3 hours Max. Marks: 75
Section- A (10 x 1 = 10 Marks) (Answer all the Question; 1 to 10)
Choose the correct answer type questions.

Two questions from each unit
Four choices with one correct answer in each question.
Answer all questions.
Section- B (5 x 7 =35 marks)

Answer all questions – Either Or type
Answers not exceeding two

pages (One question from each
unit)
Question Nos.
11a or 11b
12a or 12b
13a or 13b
14a or 14b
15a or 15b
11
Section - C (3 x 10 = 30 Marks)
Answer Any Three out of Five
Answer not exceeding four pages (One question from each

UNIT)
Question Nos. 16 - 20
The pattern of internal valuation may be:
a) Two internal test of 15 marks each: Average = 15 marks

b) Group Discussion / Seminar / Quiz = 05 Marks
c) Two assignments: 5 marks each = 05 marks
CORE PRACTICAL - QUESTION PAPER PATTERN
Time : 6 hours Maximum Marks (University Exam)
- 60 Major Practical - 1 : 20 Marks

Minor Practical - 1 :15 Marks
Spotters - 2 : 2 x 2.5 = 5 Marks
Record : 5 Marks
Viva voce : 15 Marks
Internal Marks : 40 Marks

Total : 100Marks
The pattern for internal valuation for 40 marks may be

Two internal tests of 25 marks : Average = 25
marks Observation Book : 10 Marks
One assignment : 05 Marks
19. Retotalling and Revaluation Provision:

Candidates may apply of retotalling and revaluation within ten days from the date of the
result published in the University website along with the required forms and fees.
20. Transitory provision

The candidates of previous scheme may be permitted to write exams in their own schemes
upto the examinations of April 2020 as a transitory provision.
21. Subjects and Paper related websites

All the subject details along with syllabus may be downloaded from the University website
www.mkuniversity.org
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Programme : B.Sc. MICROBIOLOGY

Programme code :
Duration : 3 years [UG]
Programme Outcomes PO1: Disciplinary Knowledge:
Acquire detailed knowledge and expertise in all the
disciplines of the subject.
PO2: Communication skills:
Able to communicate scientific information,
concepts, experiments and significance.
PO3: Ethical value:
Apply knowledge on ethical and legal based issues
PO4: Analytical reasoning:
Familiarize to collect, analyze and interpret
scientific data.
PO5: Contribution to society:
Solve public issues concerned with public health
and safety for the welfare of the society.
PO6: Scientific reasoning
Solve problems understanding the issues, and find
solutions, in day to day life.
PO7 : Employability skill
Equip with skills, based on current trends and future
expectations for career development and
placements.
PO8: Entrepreneurial skill
Equip with skills and competency to become a
successful entrepreneur.
PO9:Research related skill
Proficient skills and competence to make a
prospective career in Research & Development.
PO10: Life long learning
Identify the need for skills necessary to be
successful in future.
PO11: Instrumentation skill
Handle laboratory experiments following safety
precautions and standards.
Programme Specific Outcome PSO-1: Placement
Prepare the students in all disciplines like
agriculture, industry- medical, pharma, dairy, hotel,
food and food processing, immunologicals,
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cosmetics, vermitechnology and water treatment for

effective and respectful placement.
PSO-2:Entrepreneur
To create effective entrepreneur by enhancing their
critical thinking, problem solving, decision making
and leadership skill that will facilitate startups and
high potential organizations.
PSO-3:Research and Development
Design and implement HR systems that comply
with good laboratory practices, following ethical
values, leading the organization towards growth and
development. .
PSO-4:Contribution to society
To contribute to the development of society and
produce microbiological products, by collaborating
with stake holders, related to the betterment of
environment and mankind at the national and global
level.
FIRST SEMESTER
Sl.NO Course Course Course Credit Overall Total Marks
Categor Code distribution Credits contact
y Hours/week CIA ES Total
E
L T P S
1 Part –I Language L 3 6 25 75 100
2 Part –II English L 3 4 25 75 100
3 Part -III CC-1 L 4 5 25 75 100
4 Part -III CC-2 P 4 5 40 60 100
5 Part -III AL-1 L 3 4 25 75 100
6 Part –IV SEC-1 (NME) L 2 2 25 75 100
7 Part –IV FC L 2 2 25 75 100
8 Part –IV AECC L 2 2 25 75 100
Total 23 30
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SECOND SEMESTER
Sl.N Course Course Course Credit Overal Total Marks

O Category Code distribution l contact
Credits Hours/week
L T P S CIA ESE Total
1 Part –I Languag L 3 6 25 75 100
e
3 Part -III CC-3 L 3 4 25 75 100
4 Part -III CC-4 P 4 5 40 60 100
5 Part -III AL-2 L 3 3 25 75 100
6 Part –IV SEC-2 L 2 2 25 75 100
(NME)
7 Part –IV SEC-3 L 2 2 25 75 100
8 Part –IV AECC-2 L 2 2 25 75 100
9 Part –IV Naan Commun 2 2 25 75 100
Mudhalvan icative
Scheme English
Total 24 30
THIRD SEMESTER
Sl.N Course Course Course Credit distribution Overa Total Marks
O Category Code ll contact
Credit Hours/
s week
1 Part –I Languag L 3 6 25 75 100
e
3 Part -III CC-5 L 4 5 25 75 100
4 Part -III CC-6 P 4 5 40 60 100
5 Part -III AL-3 L 3 3 25 75 100
6 Part –IV SEC-4 L 2 2 25 75 100
7 Part –IV SEC-5 L 2 2 25 75 100
8 Part –IV AECC-3 L 2 2 25 75 100
9 Part –IV E.V.S L - 1 25 75 100
Total 23 30
FOURTH SEMESTER
Sl.NO Course Course Course Credit distribution Overall Total Marks
Category Code Credits contact
L T P S Hours/ week CIA ESE Total
1 Part –I Language L 3 6 25 75 100
15

3 Part –III CC VII L 3 3 25 75 100
4 Part –III CC VIII P 4 4 40 60 100
5 Part –III AL IV L 2 3 25 75 100
6 Part –IV SEC-6 L 2 2 25 75 100
7 Part –IV SEC-7 L 2 2 25 75 100
8 Part –IV AECC-4 L 2 2 25 75 100
9 Part –IV EVS L 2 2 25 75 100
10 Part –IV Naan Office 2 2 25 75 100
Mudhalvan Fundame
Scheme ntals
Total 25 30
FIFTH SEMESTER
Sl. Course Course Course Credit distribution Overall Total contact Marks
NO Category Code Credits Hours/week
1 Part -III CC- IX L 4 5 25 75 100
2 Part –III CC –X L 4 5 25 75 100
3 Part -III CC- XI P 4 5 40 60 100

4 Part -III Core course/ 4 5 25 75 100
Project with
viva- voce-
XII
5 Part -III Elective-5 L 3 4 25 75 100
7 Part -IV Value 2 2 25 75 100
Education
8 Part -IV Internship/ 2 - 25 75 100
Industrial
visit/ Field
visit
Total 26 30
SIXTH SEMESTER
Course Course Course Credit distribution Overall Total Marks
Category Code Credits contact
Hours/week
1 Part -III CC-XIII L 4 6 25 75 100

2 Part -III CC-XIV L 4 6 25 75 100
3 Part -III CC-XV P 4 6 40 60 100
16
6 Part -IV Extension 1 - - - -

activity
7 Part -IV Professional L 2 2 25 75 100

competency
skill
8 Part -IV Naan Medical 2 2 25 75 100
Mudhalvan Coding
Scheme
Total 21 30
Credit Distribution for UG MICROBIOLOGY

S.No Part Course Details Credit
1 III Core (11x4= 44) (4x3=12) (2x2=4) 60
2 Elective Generic/ Discipline Specific Elective (6x3=18 22
+1x4=4)
(2x1=2) 2
3 I& II Language & English 24
(Lang - 4x3=12
Eng - 4x3=12)
4 NME(2x2) 4
5 EVS(1x2) 2
6 Value Education(1x2) 2
7 Extension Activity(1x1) 1
8  Ability Enhancement [AECC]- Soft Skill(4x2=8) 8
IV  Skill Enhancement Course [4 Courses x 2 credits 9
=8 credits ] SEC-4 – 1 Credit
 Summer internship/ Industrial training (2x1=2 2
credits)
 Foundation course 2
 Professional Competency Skill 2
 Naan Mudhalvan Scheme 6
141
17
First Year
Semester-1
Subject Subject Name Category L T P S Cr Inst. Marks
Code edi Hours CIA Exter Total
ts nal
FUNDAMENTALS Core Y - - - 4 5 25 75 100
OF Course –
MICROBIOLOGY 1
AND
MICROBIAL
DIVERSITY
Course Objectives
CO1 Learn the fundamental principles about different aspects of Microbiology including recent
developments in the area.
CO2 Describe the structural organization, morphology and reproduction of microbes.
CO3 Explain the methods of cultivation of microbes and measurement of growth.
CO4 Understand the microscopy and other basic laboratory techniques – culturing, disinfection
and sterilization in Microbiology.
CO5 Compare and contrast the different methods of sterilization.
UNIT Details No.of Course
Hours Objectives
I History and Evolution of Microbiology, Classification – Three 12 CO1
kingdom, five kingdom, six kingdom and eight kingdom.
Microbial biodiversity: Introduction to microbial biodiversity-
ecological niche. Basic concepts of Eubacteria, Archaebacteria
and Eucarya. Conservation of Biodiversity.
II General characteristics of cellular microorganisms (Bacteria, 12 CO2
Algae, Fungi and Protozoa) and acellular microorganisms -
(Viruses, Viroids, Prions), Differences between prokaryotic and
eukaryotic microorganisms. Structure of Bacterial cell wall, cell
membrane, capsule, flagella, pili, mesosomes, chlorosomes,
phycobilisomes, spores, and gas vesicles. Structure of fungi
(Mold and Yeast), Structure of microalgae.
III Bacterial culture media and pure culture techniques. Mode of 12 CO3
cell division, Quantitative measurement of growth. Anaerobic
culture techniques.
IV Microscopy – Simple, bright field, dark field, phase contrast, 12 CO4
fluorescent, electron microscope – TEM & SEM, Confocal
microscopy, and Atomic Force Microscopy. Stains and staining
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methods.
V Sterilization–moist heat - autoclaving, dry heat – Hot air oven, 12 CO5
radiation – UV, Ionization, filtration – membrane filter and
disinfection, antiseptic; Antimicrobial agents.
Total 60
Course Outcomes
Course On completion of this course, students will;
Outcomes
CO1 Study the historical events that led to the discoveries and PO5, PO6, PO10
inventions and understand the Classification of
Microorganisms.
CO2 Gain Knowledge of detailed structure and functions of PO10
prokaryotic cell organelles.
CO3 Understand the various microbiological techniques, different PO11
types of media, and techniques involved in culturing
microorganisms.
CO4 Explain the principles and working mechanism of different PO4, PO11
microscopes/Microscope, their function and scope of
application.
CO5 Understand the concept of asepsis and modes of sterilization PO4, PO11
and disinfectants.
Text Books
Pelczar.M. J., Chan E.C.S. and Noel. R.K. (2007). Microbiology. 7thEdition.,McGraw –
1
Hill, New York.
Willey J., Sherwood L., and Woolverton C. J., (2017). Prescott’s Microbiology. 10th
2
Edition., McGraw-Hill International edition.
Tortora, G.J., Funke, B.R., Case,C.L. (2013). Microbiology. An Introduction 11thEdition.,
3
A La Carte Pearson.
Salle. A.J (1992). Fundamental Principles of Bacteriology. 7thEdition., McGraw Hill
4
Inc.New York.
Boyd, R.F. (1998). General Microbiology,2ndEdition., Times Mirror, Mosby
5
CollegePublishing, St Louis.
References Books
1 Jeffrey C. Pommerville., Alcamo’s Fundamentals of Microbiology (9thEdition). Jones
&Bartlett learning 2010.
2 Stanier R.Y, Ingraham J. L., Wheelis M. L., and Painter R. R. (2010). General
Microbiology, 5thEdition., MacMillan Press Ltd
3 Tortora, G.J., Funke, B.R. and, Case, C.L (2013). Microbiology-An Introduction,
11thEdition., Benjamin Cummings.
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4 Nester E., Anderson D., Roberts C. E., and Nester M. (2006). Microbiology-A Human
Perspective, 5thEdition., McGraw Hill Publications.
5 Madigan M.T., Martinko J.M., Stahl D.A, and Clark D. P. (2010). Brock - Biology of
Microorganisms, 13th Edition Benjamin-Cummings Pub Co.
Web Resources
https://www.cliffsnotes.com/study-guides/biology/microbiology/introduction-to-
1
microbiology/a-brief-history-of-microbiology
2 https://www.keyence.com/ss/products/microscope/bz-x/study/principle/structure.jsp
3 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6604941/#
4 https://bio.libretexts.org/@go/page/9188
https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-
5
nutrition/
Methods of Evaluation
Continuous Internal Assessment Test
Internal Assignments
25 Marks
Evaluation Seminars
Attendance and Class Participation
External
End Semester Examination 75 Marks
Evaluation
Total 100 Marks
Methods of Assessment
Recall (K1) Simple definitions, MCQ, Recall steps, Concept definitions
Understand/
Comprehend MCQ, True/False, Short essays, Concept explanations, Short summary or overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems, Observe,
(K3) Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate between
Analyze (K4)
various ideas, Map knowledge
Evaluate
Longer essay/ Evaluation essay, Critique or justify with pros and cons
(K5)
Check knowledge in specific or offbeat situations, Discussion, Debating or
Create (K6)
Presentations
20
Mapping with Programme Outcomes:

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 M M M
CO2 M M
CO3 S
CO4 M S
CO5 M S
Subject Subject Name Category L T P S Cr Inst. Marks

Code edi Hou CIA External Total
ts rs
PRACTICAL I - Core - - Y - 4 5 40 60 100
FUNDAMENTALS Course
OF II-
MICROBIOLOGY Practical
AND MICROBIAL
I
DIVERSITY
Course Objectives
CO1 Acquire knowledge on Cleaning of glass wares, GLP and sterilization.
CO2 Gain knowledge on media preparation and cultural characteristics.
CO3 Learn the pure culture technique
CO4 Learn the microscopic techniques and staining methods.
CO5 Acquire knowledge on stain and staining methods
Hours Objectives
I Cleaning of glass wares, Microbiological good laboratory 12 CO1
practice and safety. Sterilization and assessment of sterility–
Autoclave, hot air oven, and membrane filtration.
II Media preparation: liquid media, solid media, semi-solid 12 CO2
media, agar slants, agar deeps, agar plates.
III Preparation of basal, differential, enriched, enrichment, 12 CO3
transport, and selective media preparation- quality control
of media, growth supporting properties, sterility check of
media.
Pure culture techniques: streak plate, pour plate, decimal
dilution.
IV Culture characteristics of microorganisms: growth on 12 CO4
different media, growth characteristics, and description.
21
Demonstration of pigment production.

Microscopy: light microscopy and bright field microscopy.
V Staining techniques: smear preparation, simple staining, 12 CO5
Gram’s staining and endospore staining.
Study on Microbial Diversity using Hay Infusion Broth-Wet
mount to show different types of microbes, hanging drop.
Total 60
Course Outcomes
Outcomes
CO1 Practice sterilization methods; learn to prepare media and their
PO4, PO7, PO8,
quality control. PO9, PO11
CO2 Learn streak plate, pour plate and serial dilution and pigment
PO4, PO7, PO8,
production of microbes. PO9
CO3 Understand Microscopy methods, different Staining PO4, PO7, PO8,
techniques and motility test. PO9, PO11
CO4 Observeculture characteristics of microorganisms. PO4, PO7, PO8,
PO9
CO5 Study on Microbial Diversity using Hay Infusion Broth-Wet PO4, PO7, PO8,
mount PO9
Text Books
James G Cappucino and N. Sherman MB(1996). A lab manual Benjamin Cummins,

1
New York 1996.
2 Kannan. N (1996). Laboratory manual in General Microbiology. Palani Publications.
3 Sundararaj T (2005). Microbiology Lab Manual (1st edition) publications.
Gunasekaran, P. (1996). Laboratory manual in Microbiology. New Age International
4
Ld., Publishers, New Delhi.
5 R C Dubey and D K Maheswari (2002). Practical Microbiology. S. Chand Publishing.
References Books
1 Atlas.R (1997). Principles of Microbiology, 2nd Edition, Wm.C.Brown publishers.
Amita J, Jyotsna A and Vimala V (2018). Microbiology Practical Manual. (1st
2
Edition). Elsevier India
3 Talib VH (2019). Handbook Medical Laboratory Technology. (2nd Edition). CBS
Wheelis M, (2010). Principles of Modern Microbiology, 1st Edition. Jones and
4
Bartlett Publication.
5 Lim D. (1998). Microbiology, 2ndEdition, WCB McGraw Hill Publications.
Web Resources
1 http://www.biologydiscussion.com/micro-biology/sterilisation-and-disinfection-
22
methods-and-principles-microbiology/24403.
2 https://www.ebooks.cambridge.org/ebook.jsf?bid=CBO9781139170635
3 https://www.grsmu.by/files/file/university/cafedry//files/essential_microbiology.pdf
4 https://microbiologyinfo.com/top-and-best-microbiology-books/
https://www.cliffsnotes.com/studyguides/biology/microbiology/introduction-to-
5
microbiology/a-brief-history-of-microbiology
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend
overview
(K2)
Application Suggest idea/concept with examples, Suggest formulae, Solve problems,
(K3) Observe, Explain
Problem-solving questions, Finish a procedure in many steps, Differentiate
Analyze (K4)
between various ideas, Map knowledge
Evaluate
(K5)
Create (K6)
Presentations
CO1 M L M L M
CO2 S L L L
CO3 S M M L M
CO4 S M L L
CO5 S M L L
23
Subject Subject Category L T P S Cre Inst. Marks

Code Name dits Hour CI Exter Total
s A nal
BASIC AND Elective Y - - - 3 4 25 75 100
CLINICAL Generic /
BIOCHEMI Discipline
STRY Specific
Elective-I
Course Objectives
CO1 Attain thorough knowledge on carbohydrates and lipids, their characteristic properties and
organization in carrying out all the living functions which constitute the life.
CO2 Explain the biological activity of amino acids and proteins.
CO3 Identify the metabolic errors in enzymes of carbohydrates and lipids.
CO4 Describe the disorders in amino acid metabolism.
CO5 Interpret the consequences, biochemical, clinical features, diagnosis and treatment of
metabolic diseases of day today life.
Hours Objectives
I Biomolecules -Carbohydrate – General properties, function, structure, 12 CO1
classification– monosaccharides (Glucose, Fructose, Galactose),
Oligoaccharides (Sucrose, Maltose, Lactose) and polysaccharides
(Starch, Glycogen,) and biological significance. Lipids – General
properties, functions, structure, classification (Simple, Derived and
Complex), Cholesterol, LDL, HDL – biological significance.
II Biomolecules - Amino acids – General properties, functions, structure, 12 CO2
classification and biological significance. Proteins– General structure,
Properties, functions, classification and biological significance.
III Disorders of Metabolism: Disorders of carbohydrate metabolism: 12 CO3
diabetes mellitus,ketoacidosis, hypoglycemia, glycogen storage
diseases, galactosemia and lactose intolerance. Disorders of lipid
metabolism: hyperlipidemia, hyperlipoproteinemia,
hypercholesterolemia, hypertriglyceridemia,sphingolipidosis.
IV Disorders of Metabolism: Disorders of amino acid 12 CO4
metabolism:alkaptonuria, phenylketonuria, phenylalaninemia,
homocystineuria, tyrosinemia, aminoacidurias.
V Evaluation of organ function tests: Assessment and clinical 12 CO5
manifestations of renal, hepatic, pancreatic, gastric and intestinal
24
functions.
Diagnostic enzymes: Principles of diagnostic enzymology. Clinical
significance of aspartate aminotransferase, alanine aminotransferase,
creatine kinase, aldolase and lactate dehydrogenase.
Total 60
Course Outcomes
Outcomes
CO1 Explain the structure, classification , biochemical functions PO1
and significance of carbohydrates and lipids
CO2 Differentiate essential and non-essential amino acids, PO1
biologically important modified amino acids and their
functions, Illustrate the role, classification of Proteins and
recognize the structural level organization of proteins, its
functions and denaturation.
CO3 Assess defective enzymes and Inborn errors. Recognize PO4, PO5, PO6
diseases related to carbohydrate and lipid metabolism.
CO4 Discuss and evaluate the pathology of aminoacid metabolic PO4, PO5, PO6
disorders.
CO5 Appraise the imbalances of enzymes in organ function and PO5, PO6, PO9
relate the role of Clinical Biochemistry in screening and
diagnosis.
Text Books
1 Satyanarayana, U. and Chakrapani, U(2014).Biochemistry,4th Edition, Made Simple Publisher.
Jain J L, Sunjay Jain and Nitin Jain (2016).Fundamentals of Biochemistry, 7th Edition, S Chand
2
Company.
AmbikaShanmugam’s (2016). Fundamentals of Biochemistry for Medical Students, 8 th Edition.
3
Wolters Kluwer India Pvt Ltd.
Vasudevan. D.M.Sreekumari.S, KannanVaidyanathan (2019). Textbook Of Biochemistry For
4
Medical Students. Kindle edition, Jaypee Brothers Medical Publishers
Jeremy M. Berg,LubertStryer, John L. Tymoczko, Gregory J. Gatto (2015). Biochemistry, 8 th
5
edition. WH Freeman publisher.
References Books
AmitKessel&Nir Ben-Tal (2018). Introduction to Proteins: structure, function and motion.
1
2ndEdition, Chapman and Hall.
David L. Nelson and Michael M. Cox (2017).Lehninger Principles of Biochemistry, 7 thEdition
2
W.H. Freeman and Co., NY.
LupertStyrer, Jeremy M. Berg, John L. Tymaczko, Gatto Jr., Gregory J (2019). Biochemistry.
3
9thEdition ,W.H.Freeman& Co. New York.
4. Donald Voet, Judith Voet, Charlotte Pratt (2016). Fundamentals of Biochemistry: Life at the
25
Molecular Level, 5th Edition, Wiley.

Joy PP, Surya S. and AswathyC (2015). Laboratory Manual of Biochemistry, Edition
5.
1.,Publisher:Kerala agricultural university.
Web Resources
1 https://www.abebooks.com › plp
2 https://kau.in/document/laboratory-manual-biochemistry
3 https://metacyc.org
4 https://www.medicalnewstoday.com
5 https://journals.indexcopernicus.com
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehen MCQ, True/False, Short essays, Concept explanations, Short summary or overview
d (K2)
(K3) Explain
Analyze Problem-solving questions, Finish a procedure in many steps, Differentiate between
(K4) various ideas, Map knowledge
Evaluate
(K5)
Create (K6)
Presentations
1
CO1 M
CO2 M
CO3 S S S
CO4 S S S
26
CO5 S S S
Subject Subject Category L T P S Credit Inst. Marks
Code Name s Hour CI Externa Total
s A l
Social Skill Y - - - 2 2 25 75 100
and enhancemen
Preventiv t Course
e SEC - 1
medicine (NME)
Course Objectives
CO1 Describe the concepts of health and disease and their social determinants
CO2 Summarize the health management system
CO3 Know about the various health care services
CO4 Outline the goals of preventive medicine
CO5 Gain knowledge about alternate medicine
Hours Objectives
I Introduction to social medicine: 6 CO1
History of social medicine-concepts of health and disease-
social determinants of health and disease-Health and quality of
life-Health information system- measures of population health-
health policies.
II Health management: 6 CO2
Applications of behavioral sciences and psychology in health
management- nutritional programs for health management-
water and sanitation in human health-national programs for
communicable and non-communicable diseases- environmental
and occupational hazards and their control.
III Health care and services: 6 CO3
Health care of the community-information, education,
communication and training in health-maternal & child health-
school health services- Geriatrics-care and welfare of the aged-
mental health-health services through general practitioners.
IV Preventive medicine: 6 CO4
Introduction- role of preventive medicine- levels of prevention-
Risk assessment in communities and vulnerable population –
surveillance, monitoring and reporting of disease outbreaks -
forecasting and control measures in community setting – early
27
detection methods.
V Prevention through alternate medicine: 6 CO5
Unani, Ayurveda, Homeopathy, Naturopathy systems in
epidemic and pandemic outbreaks. International health
regulations. Infectious disease outbreak case studies and
precautionary response during SARS and MERS coronavirus,
Ebola and novel SARS-COV2 outbreaks.
Total 30
Course Outcomes
Outcomes
CO1 Identify the health information system PO1,PO5, PO6
CO2 Associate various factors with health management system PO1,PO2, PO3,PO5, PO6,
PO9
CO3 Choose the appropriate health care services PO1,PO5, PO6
CO4 Appraise the role of preventive medicine in community PO4,PO5, PO6
setting
CO5 Recommend the usage of alternate medicine during PO1,PO5, PO6
outbreaks
Text Books
1. Park.K (2021). Textbook of preventive and social medicine, 26th edition.
BanarsidasBhanot publishers.
2. Mahajan& Gupta (2013). Text book of preventive and social medicine, 4thedition.
Jaypeebrothers medical publishers.
3. Chun-Su Yuan, Eric J. Bieber, Brent Bauer (2006). Textbook of Complementary and
Alternative Medicine. Second Edition. Routledge publishers.
4. Vivek Jain (2020). Review of Preventive and Social Medicine: Including Biostatics. 12th
edition,Jaypee Brothers Medical Publishers.
5. LalAdarshPankaj Sunder (2011). Textbook of Community Medicine: Preventive and
Social Medicine, CBS publisher.
References Books
1 Howard Waitzkin, Alina Pérez, Matt Anderson (2021). Social Medicine and the coming
Transformation. First Edition. Routledge publishers.
2 GN Prabhakara (2010). Short Textbook of Preventive and Social Medicine. Second
Edition. Jaypee publishers.
3 Jerry M. Suls, Karina W. Davidson, Robert M. Kaplan (2010).Handbook of Health
Psychology and BehavioralMedicine.Guilford Press.
4 Marie Eloïse Muller, Marie Muller, MarthieBezuidenhout, KarienJooste (2006).Health
Care Service Management. Juta and Company Ltd.
5 Geoffrey Rose (2008).Rose's Strategy of Preventive Medicine: The Complete.OUP
28
Oxford.
Web Resources
1 https://www.omicsonline.org/scholarly/social--preventive-medicine-journals-articles-
ppts-list.php
2 https://www.teacheron.com/online-md_preventive_and_social_medicine-tutors
3 https://www.futurelearn.com
4 https://www.healthcare-management-degree.net
5 https://www.conestogac.on.health-care-administration-and-service-management
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand
/
MCQ, True/False, Short essays, Concept explanations, Short summary or overview
Comprehen
d (K2)
(K3) Explain
Analyze Problem-solving questions, Finish a procedure in many steps, Differentiate between
Evaluate
(K5)
Create (K6)
Presentations
CO1 S S S
CO2 S S M S S M
CO3 M S S
CO4 S S S M
CO5 S S S
29
SEMESTER II
Subject Subject Name Category L T P S Cre Inst. Marks

Code dits Hour CI Exter Total
s A nal
MICROBIAL Core Y - - - 3 4 25 75 100
PHYSIOLOGY Course III
AND
METABOLISM
Course Objectives
CO1 Study the basic principles of microbial growth.
CO2 Understand the basic concepts of aerobic and anaerobic metabolic pathways.
CO3 Analyze the role of individual components in overall cell function.
CO4 Provide information on sources of energy and its utilization by microorganisms.
CO5 Study the different types of metabolic strategies.
Unit Details No.of Course
Hours Objectives
I Physiology of microbial growth: Batch – continuous - synchronous 12 CO1
cultures; Growth Curve and measurement method (turbidity,
biomass, and cell count). Control of microbial growth.
II Nutrition requirements - Photoautotrophs, Photoorganotrophs, 12 CO2
Chemolithotrophs (Ammonia, Nitrite, Sulfur, Hydrogen, Iron
oxidizing Bacteria), Chemoorganotrophs. Nutrition transport
mechanisms – Passive diffusion and Active transport. Factors
affecting microbial growth.
III An overview of Metabolism - Embden Meyerhof Pathway, Entner- 12 CO3
Doudoroff Pathway, Pentose Phosphate Pathway, Tricarboxylic
Acid Cycle. Electron Transport Chain and Oxidative
Phosphorylation. ATP synthesis. Fermentation-Homolactic
Fermentation, Heterolactic Fermentation, Mixed Acid Fermentation,
Butanediol Fermentation.
IV Photosynthesis - An Overview of chloroplast structure. 12 CO4
Photosynthetic Pigments, Light Reaction-Cyclic and non-cyclic
Photophosphorylation. Dark Reaction - Calvin Cycle.
V Bacterial reproduction - Binary fission, Budding, Reproduction 12 CO5
30
through conidia, cyst formation, endospore formation. Fungi asexual

and sexual reproduction, Microalgae reproduction. Asexual and
sexual reproduction of protozoa.
Total 60
Course Outcomes
Outcomes
CO1 Describe microorganisms based on nutrition. PO6, PO9
CO2 Know the concept of microbial growth and identify the factors PO6, PO7, PO9
affecting bacterial growth.
CO3 Explain the methods of nutrient uptake. PO6, PO9
CO4 Describe anaerobic and aerobic energy production. PO6, PO9
CO5 Elaborate on the process of bacterial photosynthesis and PO6, PO9
reproduction.
Text Books
Schlegal, H.G. (1993). General Microbiology.,7th Edition, Press syndicate of the
1
University of Cambridge.
2 RajapandianK.(2010). Microbial Physiology, Chennai: PBS Book Enterprises India.
3 MeenaKumari. S. Microbial Physiology, Chennai 1st Edition MJP Publishers 2006.
Dubey R.C. and Maheswari, S. (2003). A textbook of Microbiology, New Delhi: S.
4
Chand & Co.
5 S. Ram Reddy, S.M. Reddy (2008). Microbial Physiology. Anmol Publications Pvt Ltd.
References Books
Robert K. Poole (2004). Advances in Microbial Physiology, Elsevier Academic Press,

1
New York, Volume 49.
Kim B.H., Gadd G.M. (2008). Bacterial Physiology and Metabolism. Cambridge
2
University Press, Cambridge.
Daniel R. Caldwell. (1995). Microbial Physiology & Metabolism Wm.C. Brown
3
Communications, Inc. USA.
Moat, A.G and J.W Foaster (1995). Microbial Physiology, 3 rd edition. Wiley – LISS, A
4
John Wiley & Sons. Inc. Publications.
5 BhanuShrivastava. (2011). Microbial Physiology and Metabolism: Study of Microbial
Physiology and Metabolism. Lambert academic Publication.
Web Resources
1 https://sites.google.com/site/microbial physiologyoddsem/teaching-contents.
2 https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-Nutrition
3 https://onlinecourses.swayam2.ac.in/cec20_bt14/preview
31
4 http://web.iitd.ac.in/~amittal/2007_Addy_Enzymes_Chapter.pdf
5 https://www..frontiersin.org.microbial-physiology-and-metabolism
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
(K2)
(K3) Explain
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations

CO1 M M
CO2 M L M
CO3 M M
CO4 M M
CO5 M M
32
Subject Subject Name Catego L T P S Cre Inst. Marks

Code ry dits Hours CIA Exter Total
nal
CCIV- - - Y - 4 5 40 60 100
MICROBIAL CORE
PHYSIOLOGY PRAC
AND TICAL
METABOLISM II
Course Objectives
CO1 Understand the principles of motility test.
CO2 Understand the basic concepts of staining methods.
CO3 Learn the bacterial count using different methods and anaerobic culture.
CO4 Study the morphological demonstration of microorganisms and identification.
CO5 Study the biochemical identification of the bacteria.
Hours Objectives
I Motility demonstration: hanging drop, wet mount preparation, semi- 12
solid agar, Craigie’s tube method. Staining techniques: Smear CO1
preparation, permanent specimen preparation, Capsular, and Acid-fast
staining
II Direct counts – Direct cell count (Petroff-Hausser counting chamber), 12 CO2
Turbidometry. Viable count - pour plate, spread plate.
Bacterial growth curve.
III Anaerobic culture methods. Antibiotic sensitivity testing: Disc 12 CO3
diffusion test- quality control with standard strains.
IV Morphological variations in algae, fungi and protozoa. Micrometry: 12 CO4
Demonstration of the size of yeast, fungal filaments and protozoa.
V Methods of bacterial identification- morphological, physiological, and 12 CO5
biochemical methods - IMViC test, H2S, TSI, Oxidase, catalase,
urease test, and Carbohydrate fermentation test.Maintenance of pure
culture, paraffin method, stab culture, maintenance of mold culture.
Total 60
Course Outcomes
Outcomes
CO1 Describe hanging drop, wet mount preparation, semi-solid agar, PO6, PO7, PO8, PO9,
Craigie’s tube method. PO11
CO2 Demonstrate Smear preparation, permanent specimen PO6, PO7, PO8, PO9,
33
preparation, Capsular, and Acid-fast staining. PO11

CO3 Explain antibiotic sensitivity testing: Disc diffusion test- quality PO6, PO7, PO8, PO9,
control with standard strains. PO11
CO4 Describe demonstration of the size of yeast, fungal filaments and PO6, PO7, PO8, PO9,
protozoa. PO11
CO5 Elaborate on the bacterial identification- morphological, PO6, PO7, PO8, PO9,
physiological, and biochemical methods. PO11
Text Books
James G Cappucino and N. Sherman MB (1996). A lab manual Benjamin Cummins, New
1
York .
2 Kannan. N (1996).Laboratory manual in General Microbiology. Palani Publications.
3 Sundararaj T (2005). Microbiology Lab Manual (1st edition) publications.
Gunasekaran. P (2007). Laboratory manual in Microbiology. New age international
4
publisher.
Elsa Cooper (2018). Microbial Physiology: A Practical Approach. Callisto Reference
5
publisher.
References Books
DavidWhite., James Drummond., Clay Fuqua (2012) Physiology and Biochemistry of
1
Prokaryotes. 4th Ed. Oxford University Press, New York.
Robert K. Poole (2004). Advances in Microbial Physiology, Elsevier Academic Press,
2
New York, Volume 49.
Kim B.H., Gadd G.M. (2008). Bacterial Physiology and Metabolism. Cambridge
3
University Press, Cambridge.
Dawes, I.W and Sutherland L.W (1992). Microbial Physiology (2nd edition), Oxford
4
Blackwell Scientific Publications.
Moat, A.G and J.W Foaster, (1995). Microbial Physiology, 3 rd edition. Wiley – LISS, A
5
John Wiley & Sons. Inc. Publications.
Web Resources
https://sites.google.com/site/microbial physiologyoddsem/teaching-contents
1
2 https://courses.lumenlearning.com/boundless-microbiology/chapter/microbial-Nutrition
3 https://onlinecourses.swayam2.ac.in/cec20_bt14/preview
4 https://www.studocu.com/microbial-physiology-practicals
5 https://www.agr.hokudai.ac.jp/microbial-physiology
40 Marks
Evaluation Seminars
External End Semester Examination 60 Marks
34
Evaluation
Total 100 Marks
Simple definitions, MCQ, Recall steps, Concept definitions

Recall (K1)
Understand/
(K2)
(K3) Explain
Analyze (K4)
Evaluate
(K5)
Create (K6) Presentations.

CO1 M L M L M
CO2 M M L M L
CO3 L M M L M
CO4 L M M M M
CO5 M M M M M
35

s A nal
BIO Elective Y - - - 3 3 25 75 100
INSTRUMENTA Generic /
TION Discipline
Specific
Elective II
Course Objectives
CO1 Understand the analytical instruments and study the basic principles in the field of sciences.
CO2 To gain knowledge about principles of spectroscopy
CO3 Understand the analytical techniques of Chromatography and electrophoresis
CO4 To understand the principle of different types of scans used in medical diagnosis
CO5 To gain information about the principles of radioactivity and its measurements
Hours Objectives
I Basicinstruments:pH meter, Buffer of biological importance, 12 CO1
Centrifuge- Preparative, Analytical and Ultra, Laminar Air Flow,
Autoclave, Hot Air Oven and Incubator. Biochemical calculations-
preparations of Molar solutions - Buffers- Phosphate, Acetate, TE,
TAE- calculation of Normality ,PPM- Ammonium sulphate
precipitation.
II Spectroscopic Techniques:Spectroscopic Techniques: Colorimeter, 12 CO2
Ultraviolet and visible, Infra red and Mass Spectroscopy.
III Chromatographic and Electrophoresis Techniques:Chromatographic 12 CO3
Techniques: Paper, Thin Layer, Column, HPLC and GC.
Electrophoresis Techniques: Starch Gel, AGE, PAGE.
IV Imaging techniques:Principle, Instrumentation and application of ECG, 12 CO4
EEG, EMG, MRI, CT and PET scan radioisotopes.
V Fluorescence and radiation based techniques:Spectrofluorimeter, Flame 12 CO5
photometer, Scintillation counter, Geiger Muller counter,
Autoradiography.
Total 60
Course Outcomes
Outcomes
CO1 Gain knowledge about the basics of instrumentation. PO1,PO4,PO11
CO2 Exemplify the structure of atoms and molecules by using the PO4,PO10,PO11
36
principles of spectroscopy.
CO3 Evaluate by separating and purifying the components. PO4,PO7,PO11
CO4 Understand the need and applications of imaging techniques. PO7,PO8,PO11
CO5 Categorize the working principle and applications of PO10,PO11
fluorescence and radiation.
Text Books
1. Jayaraman J (2011). Laboratory Manual in Biochemistry, 2nd Edition. Wiley Eastern Ltd.,
New Delhi .
2. Ponmurugan. P and Gangathara PB (2012). Biotechniques.1stEdition. MJP publishers.
3 Veerakumari, L (2009).Bioinstrumentation- 5 thEdition -.MJP publishers.
4 Upadhyay, Upadhyay and Nath (2002). Biophysical chemistry – Principles and techniques 3rd
Edition. Himalaya publishing home.
5 Chatwal G and Anand (1989). Instrumental Methods of Chemical Analysis. S.Himalaya
Publishing House, Mumbai.
References Books
1 Rodney.F.Boyer (2000). Modern Experimental Biochemistry, 3rd Edition. Pearson
Publication.
2 SkoogA.,WestM (2014). Principles of Instrumental Analysis – 14th Edition
W.B.SaundersCo.,Philadephia.
3 N.Gurumani. (2006). Research Methodology for biological sciences- 1st Edition – MJP
Publishers .
4 Wilson K, and Walker J (2010). Principles and Techniques of Biochemistry and Molecular
Biology.7thEdition. Cambridge University Press .
5 Webster, J.G. (2004). Bioinstrumentation- 4th Edition - John Wiley & Sons (Asia)
Pvt.Ltd,Singapore.
Web Resources
1 http://www.biologydiscussion.com/biochemistry/centrifugation/centrifugeintroduction-
typesuses-and-other-details-with-diagram/12489
2 https://www.watelectrical.com/biosensors-types-its-working-andapplications/
3 http://www.wikiscales.com/articles/electronic-analytical-balance/ Page 24 of 75
4 https://study.com/academy/lesson/what-is-chromatography-definition-typesuses.html
5 http://www.rsc.org/learn-chemistry/collections/spectroscopy/introduction
25 Marks
Evaluation Seminars
37
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
(K3) Explain
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO1 L M S
CO2 L M S
CO3 L M S
CO4 S S S
CO5 M S
38

s A nal
Nutrition & Skill Y - - - 2 2 25 75 100
Health Hygiene Enhance
ment
Course -
SEC-2
(NME)
Course Objectives
CO1 Learn about nutrition and their importance
CO2 Make student understand thenutritionalfacts fora better life.
CO3 Learn information to optimize our diet
CO4 Impart knowledge on different health care programs taken up by India
CO5 Learn knowledge on different health indicators and types of hygiene methods
Hour Objectives
s
I Nutrition – definition, importance, Good nutrition, and mal nutrition; 5 CO1
Balanced Diet: Basics of Meal Planning. Carbohydrates, Lipids, Proteins
and Vitamins –functions, dietary sources, effects of deficiency. Macro
and micro minerals –functions, effects of deficiency; food sources of
Calcium, Potassium, and Sodium; food sources of Iron, Iodine, and Zinc.
Importance of water– functions, sources, requirements and effects of
deficiency
II Nutrition for Life Cycle: Balanced diet - Normal, Pregnant, lactating 5 CO2
women, Infancy, young children Adolescents, Adults, and the Elderly;
Diet Chart; Nutritive value of Indian foods.
III Improper diets: Definition, Identification, Signs and Symptoms - 5 CO3
malnutrition, under-nutrition, over-nutrition, Protein Energy
Malnutrition, obesity; Nutritional Disease and Disorder - hypertension,
diabetes, anemia, osteomalacia, cardiovascular disease.
IV Health - Determinants of health, Key Health Indicators, Environment 5 CO4
health & Public health; Health-Education: Principles and Strategies.
Health Policy & Health Organizations: Health Indicators and National
Health Policy of Govt. of India; Functioning of various nutrition and
health organizations in India.
V Hygiene – Definition; Personal, Community, Medical and Culinary 5 CO5
hygiene; WASH (Water, Sanitation and Hygiene) programme. Rural
39
Community Health: Village health sanitation & Nutritional committee.

Community & Personal Hygiene: Environmental Sanitation and
Sanitation in Public places.
Total 25
Course Outcomes

Outcomes
CO1 Learn the importance of nutrition for a healthy life PO5, PO6, PO7, PO8, PO10
CO2 Study the nutrition for life cycle PO5, PO6, PO7, PO8, PO10
CO3 Know the health care programmes of India PO5, PO6, PO7, PO8, PO10
CO4 Learn the importance of community and personal health & PO5, PO6, PO7, PO10
hygiene measures
CO5 Create awareness on community health and hygiene PO5, PO6, PO7, PO10
Text Books
1. Bamji, M.S., K. Krishnaswamy& G.N.V. Brahmam (2009) Textbook of Human
Nutrition(3rd edition) Oxford and IBH Publishing Co. Pvt. Ltd., New Delhi
2. Swaminathan (1995)Food &Nutrition(Vol I, Second Edition) The Bangalore Printing
&Publishing Co Ltd., , Bangalore
3 SK. Haldar(2022). Occupational Health and Hygiene in Industry. CBS Publishers.
4 Acharya, Sankar Kr, Rama Das, MinatiSen (2021). Health Hygiene and Nutrition Perception
and Practices.Satish Serial Publishing House
5 Dass (2021).Public Health and Hygiene, Notion Press
References Books
1 VijayaKhader (2000)Food, nutrition & health, Kalyan Publishers, New Delhi
2 Srilakshmi, B., (2010)Food Science, (5th Edition) New Age International Ltd., New Delhi
3 Arvind Kumar Goel (2005). A College Textbook of Health &Hygiene,ABD Publishers
4 Sharma D. (2015).Textbook on Food Science and Human Nutrition.Daya Publishing
House.
5 Revilla M. K. F., Titchenal A. and Draper J. (2020).Human Nutrition.
University of Hawaii, Mānoa.
Web Resources
1 National Rural Health Scheme:
https://nhm.gov.in/index1.php?lang=1&level=1&sublinkid=969&lid=49
2 National Urban Health Scheme:
3 Village health sanitation & Nutritional committee
4 Health Impact Assessment - https://www.who.int/hia/about/faq/en/
5 Healthy Living https://www.nhp.gov.in/healthylivingViewall
40
Continuous Internal Assessment Test 25 Marks
Evaluation Seminars
Evaluation
Total 100 Marks
Recall (KI) Simple definitions, MCQ, Recall steps, Concept definitions

Understand /
(K2)
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate
Evaluate (K5) Longer essay/ Evaluation essay, Critique or justify with pros and cons
Create (K6) Check knowledge in specific or offbeat situations, Discussion, Debating or

Presentations
Mapping with Programme Outcomes
CO1 S M M M S
CO2 S M M M S
CO3 S M M M S
CO4 S S L S
CO5 S S M S
41
Subject Subject Category L T P S Cre Inst. Marks

Code Name dits Hour CI Exter Total
s A nal
SERICULT Skill Y - - - 2 2 25 75 100
URE Enhanceme
nt Course -
SEC-3
Course Objectives
CO1 Acquire knowledge on the concepts of origin, growth and study of Sericulture as science
and scientific approach of mulberry plant.
CO2 Describe the morphology and physiology of silkworm.
CO3 Discuss effective management of silkworm diseases.
CO4 Demonstrate field skills in mulberry cultivation and silkworm rearing with an emphasis on
technological aspects.
CO5 Demonstrate entrepreneurship abilities, innovative thinking, planning, and setting up small-
scale enterprises.
Hours Objectives
I General introduction to Sericulture, its distribution in India.
Botanical distribution and taxonomical characters of mulberry
5 CO1
varieties and species.Biology of Mulberry plant and Mulberry crop
cultivation and protection.
II Silkworm- biology-morphology of silkworm. Life cycle of
silkworm- egg, larva, pupa, and moth. 5 CO2
III Silkworm pathology: Introduction to Parasitism, Commensalism,

Symbiosis and Parasite relationship - Mulberry Silkworm Diseases:
Introduction, types, Pebrine, Grasserie, Muscardine, Flacherie,
Symptoms and Pathogens, Mode of Infection, Prevention and 5 CO3
Control -Non – mulberry silkworm diseases: Pebrine, Bacterial and
viral diseases. Brief Account of Pests and Predators of Silkworms,
Nature of damage and control measures.
IV Rearing of silkworm. Cocoon assessment and processing
5 CO4
technologies. Value added products of mulberry and silkworms.
V Entrepreneurship and rural development in sericulture:Planning for
EDP, Project formulation, Marketing, Insectary facilities and
equipments: Location, building specification, air conditioning and 5 CO5
environmental control, furnishings and equipment, sanitation and
equipment, subsidiary facilities.
Total 25
42
Course Outcomes
Outcomes
CO1 Discuss the overall aspects of Sericulture and the biology and PO1,PO5,PO7
varieties of mulberry plant.Creates awareness among students
about the economic importance and suitability of Sericulture in
Indian conditions.
CO2 Familiarize with the lifecycle of silk worm. PO1, PO2

Explain common diseases of silkworm encountered during
CO3 rearing, sources of infection, disease symptoms, pre-disposing PO1, PO5
factors and their management practices.
Attain thorough knowledge about the cultivation of mulberry,
CO4 maintenance of the farm, seed technology, silkworm rearing, post PO7, PO8, PO10
cocoon techniques like stifling, reeling, and utilization of by-
products.
Plan the facilities required for establishment of insectary.
CO5 Competent to transfer the knowledge and technical skills to the PO5, PO7, PO8
Seri-farmers.Analyze the importance of sericulture in
entrepreneurship development and emerge as potential
entrepreneur.
Text Books
1 Ganga, G. and SulochanaChetty(2010). Introduction to Sericulture,, J., Oxford and IBH
Pub. Co. Pvt. Ltd., New Delhi.
2 Dr. R. K. Rajan&Dr. M. T. Himantharaj(2005). Silkworm Rearing Technology, Central Silk
Board, Bangalore.
3 Dandin S B, JayantJayaswal and Giridhar K (2010). Handbook of Sericulture
technologies,Central Silk Board, Bangalore.
4 M. C. Devaiah, K. C. Narayanaswamy and V. G. Maribashetty(2010). Advances in
Mulberry Sericulture,,CVG Publications, Bangalore
5 T.V.SatheandJadhav.A.D.(2021). Sericulture and Pest Management, Daya Publishing
House.
References Books
1 S. Morohoshi (2001). Development Physiology of Silkworms 2ndEdition, Oxford & IBH
Publishing Co. Pvt. Ltd. New Delhi
2 Hamamura, Y (2001). Silkworm rearing on Artificial Diet. Oxford & IBH publishing Co.,
Pvt. Ltd. NewDelhi.
3 M.Johnson, M.Kesary (2019).Sericulture, 5th.Edition.Saras Publications.
4 Manisha Bhattacharyya (2019).Economics of Sericulture, Rajesh Publications.
43
5 Muzafar Ahmad Bhat, SurakshaChanotra, ZafarIqbalBuhroo, Abdul Aziz and

Mohd.Azam (2020).A Textbook on Entrepreneurship Development Programme in
Sericulture, IP Innovative Publication.
Web Resources
1 https://egyankosh.ac.in › bitstream
2 https://archive.org › details › SericultureHandbook
3 https://www.academic.oup.com
4 https://www.sericulture.karnataka.gov.in
5 https://www.silks.csb.gov.in
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
(K2)
(K3) Explain
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO1 S S S
CO2 M S
CO3 S S
CO4 S S S
CO5 S S S
44
SEMESTER III
Subject Subject Name Category L T P S Credits Inst. Marks

Code Hours
CIA External Total
Molecular Biology Core 4 1 - - 4 5 25 75 100
and Microbial Course V
Genetics -Theory
Learning Objectives
CO1 Provide knowledge on structure and replication of DNA.
CO2 Illustrate the significance and functions of RNA in protein synthesis.
CO3 Explain the cause and types of DNA mutation and DNA repair mechanisms.
CO4 Outline the role of plasmids and phages in genetics.
CO5 Examine mechanisms of gene transfer and recombination.
Unit Details No. of Course
Hours Objectives
I DNA Structure - Salient features of double helix, forms of DNA. 15 CO1
Denaturation and renaturation. DNA topology – Supercoiling,
linking number, topoisomerases. DNA organization in prokaryotes,
viruses, eukaryotes. Replication of DNA in prokaryotes and
eukaryotes - Bidirectional and unidirectional replication, semi-
conservative and semi-discontinuous replication. Mechanism of
DNA replication – enzymes involved – DNA polymerases, DNA
ligase, primase. DNA replication modes - rolling circle, D-loop
modes.
II Transcription in Prokaryotes. Concept of transcription. RNA 15 CO2
Polymerases - prokaryotic and eukaryotic. General transcription
factors in eukaryotes. Distinction between transcription processes in
prokaryotes versus eukaryotes. Translation in prokaryotes and
eukaryotes - Translational machinery - ribosome structure in
prokaryotes and eukaryotes, tRNA structure and processing.
Inhibitors of protein synthesis in prokaryotes and eukaryotes.
Overview of regulation of gene expression - lac, trp and ara operons
as examples. Regulation of gene expression by DNA methylation.
III Mutation - Definition and types - base substitutions, frame shifts, 15 CO3
deletions, insertions, duplications, inversions. Silent, conditional,
and lethal mutations. Physical and chemical mutagens. Reversion
and suppression. Uses of mutations. Repair Mechanisms -
Photoreactivation, Nucleotide Repair, Base Excision Repair, Methyl
Directed Mismatch Repair and SOS Repair.
45
IV Plasmid replication and partitioning, host range, plasmid 15 CO4

incompatibility, plasmid amplification, regulation of plasmid copy
number, curing of plasmids. Types of plasmids – R Plasmids, F
plasmids, colicinogenic plasmids, metal resistance plasmids, Ti
plasmid, linear plasmids, yeast 2µ plasmid. Bacteriophage-T4,
Virulent Phage – Structure and lifecycle. Lambda phage-Structure,
Lytic and Lysogenic cycle. Applications of Phages in Microbial
Genetics.
V Gene Transfer Mechanisms- Conjugation and its uses. Transduction 15 CO5
- Generalized and Specialized, Transformation - Natural
Competence and Transformation. Transposition and Types of
Transposition reactions. Mechanism of transposition: Replicative
and non- replicative transposition. Transposable elements -
Prokaryotic transposable elements – insertion sequences, composite,
and non-composite transposons. Uses of transposons.
Total 75
Course Outcomes
Outcomes
CO1 Analyze the significance of DNA and elucidate the replication PO4, PO5,
mechanism. PO7,PO9
CO2 Illustrate the types of RNA and protein synthesis machinery. PO4, PO7,PO9
CO3 Infer the causes and types of DNA mutation and summarize the PO5, PO7,PO9
DNA repair mechanisms.
CO4 Evaluate the importance of plasmids and phages in genetics. PO7,PO9
CO5 Analyze gene transfer and recombination methods. PO5, PO6,
PO7,PO9
Text Books
1. Malacinski G.M. (2008). Freifelder’s Essentials of Molecular Biology. 4th Edition. Narosa
Publishing House, New Delhi.
2. Gardner E. J. Simmons M. J. and SnustedD.P.(2006). Principles of Genetics. 8th Edition.
Wiley India Pvt. Ltd.
3. Trun N. and Trempy J. (2009). Fundamental Bacterial Genetics. 1st Edition. Blackwell
Science Ltd.
4. Brown T. A. (2016). Gene Cloning and DNA Analysis- An Introduction. (7th Edition). John
Wiley and Sons, Ltd.
5. Dale J. W., Schantz M.V. and Plant N. (2012). From Gene to Genomes – Concepts and
Applications of DNA Technology. (3rd Edition). John Wileys and Sons Ltd.
References Books
1. Glick B. R. and Patten C.L. (2018). Molecular Biotechnology – Principles and Applications
of Recombinant DNA. 5th Edition. ASM Press.
2. Russell P.J. (2010). iGenetics - A Molecular Approach, 3rd Edition., Pearson New
46
International edn.
3. Nelson, D.L. and Cox, M.M. Lehninger(2017). Principles of Biochemistry. 7 th Edition, W.H.
Freeman.
4. Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics of
Bacteria, 4th Edition, ASM Press Washington-D.C. ASM Press.
5. Primrose S.B. and Twyman R. M. (2006). Principles of Gene Manipulation and Genomics.
(7th Edition). Blackwell Publishing
Web Resources
1. [PDF] Lehninger Principles of Biochemistry (8th Edition) By David L. Nelson and Michael
M. Cox Book Free Download - StudyMaterialz.in
2. https://microbenotes.com/gene-cloning-requirements-principle-steps-applications/
3. https://courses.lumenlearning.com/boundless-biology/chapter/dna-replication/
4. Molecular Biology Notes - Microbe Notes

5. Molecular Biology Lecture Notes & Study Materials | Easy Biology Class
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10
CO1 S S M S M S M
CO2 S M M S M S L
CO3 M S M S M S L
CO4 M M M S M S L
47
CO5 M S S S M S L

Code Hours
CIA Extern Total
al
Molecular Biology Core - - Y - 4 5 40 60 100
Course –VI
and Microbial
– Practical
Genetics III
Learning Objectives
CO1 Provide knowledge on structure and replication of DNA.
CO2 Elucidate the methods of Genomic and Plasmid DNA isolation.
CO3 Explain methods of protein separation.
CO4 Explain artificial transformation method.
CO5 Outline the role of phages in genetics.
Hours Objecti
ves
I Study of different types of DNA and RNA using micrographs and 15 CO1
model / schematic representations.
Study of semi-conservative replication of DNA through micrographs
/ schematic representations.
II Isolation of Genomic and Plasmid DNA from E. coli and Analysis 15 CO2
by Agarose gel electrophoresis.
Estimation of DNA using colorimeter (diphenylamine reagent), UV
spectrophotometer (A260 measurement).
III Resolution and visualization of proteins by polyacrylamide gel 15 CO3
electrophoresis (SDS-PAGE) – Demonstration.
UV induced auxotrophic mutant production and isolation of mutants
by replica plating technique – Demonstration.
IV Perform artificial Transformation in E. coli. 15 CO4
Isolation of antibiotic resistant mutants by gradient plate method. -
Demonstration
V Screening and isolation of phages from sewage. 15 CO5
Perform RNA isolation.
Estimate RNA.
Total 75
48
Course Outcomes
Outcomes
CO1 Illustrate different types of DNA and RNA. PO4, PO7, PO9, PO11
CO2 Utilize hands-on training in isolation of genomic and PO4, PO7, PO9, PO11
plasmid DNA.
CO3 Analyze importance of experimental microbial genetics. PO4, PO7, PO9, PO11
CO4 Apply the knowledge of molecular techniques in various PO4, PO7, PO9, PO11
fields.
CO5 Investigate the significance of Phages. PO4, PO7, PO9, PO11
Text Books
1. Crichton. M. (2014). Essentials of Biotechnology. Scientific International Pvt Ltd.New
Delhi.
2. Sambrook J. and Russell D.W. (2001). Molecular Cloning - A Laboratory Manual – 7 th
Edition. Cold Spring Harbor, N.Y: Cold Spring Harbor Laboratory Press.
3. Dale J. W., Schantz M. V. and Plant N. (2012). From Gene to Genomes – Concepts and
Applications of DNA Technology. (3rd Edition). John Wileys and Sons Ltd.
4. Gunasekaran P. (2007). Laboratory Manual in Microbiology. New Age International.
5. James G Cappucino. and Natalie Sherman. (2016). Microbiology – A laboratory manual.
(5th Edition). The Benjamin publishing company. New York.
References Books
1 Glick B. R. and Patten C.L. Molecular Biotechnology – Principles and Applications of
Recombinant DNA. 5th Edition. ASM Press. 2018.
2 Russell P.J. (2010). iGenetics - A Molecular Approach, 3 rd Edition., Pearson New
International edn.
3 Nelson, D.L. and Cox, M.M. Lehninger(2017). Principles of Biochemistry. 7th Edition,
W.H. Freeman.
4 Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics of
Bacteria, 4th edition, ASM Press Washington-D.C. ASM Press.
5 Brown T.A. (2016). Gene Cloning and DNA Analysis. (7th Edition). John Wiley and Jones,
Ltd.
Web Resources
1 https://www.molbiotools.com/usefullinks.html
2 (PDF) Molecular Biology Laboratory manual (researchgate.net)
3 https://www.molbiotools.com/usefullinks.html
4 https://geneticgenie.org3.
5 https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpet.5
25 Marks
Evaluation Seminars
49
External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO1 S L M S M S M S
CO2 S L M S M S M S
CO3 S L M S M S M S
CO4 S L M S M S M S
CO5 S L M S M S M S
50

Code dits Hour CIA Exte Total
s rnal
CLINICAL ELECTIVE Y - - - 3 4 25 75 100
LABORATOR GENERIC/D
Y ISCIPLINE
TECHNOLOG SPECIFIC
Y ELECTIVE
-III
Learning Objectives
CO1 Demonstrate ethical and professional conduct with patients, laboratory personnel, healthcare
professionals, and the public.
CO2 Explain how accurate and reliable information might be obtained about proper procurement,
storage, and handling of laboratory specimens.
CO3 Develop a sound scientific knowledge foundation that prepares them to interpret, analyze and
evaluate scientific knowledge in clinical practice.
CO4 Perform a full range of laboratory tests with accuracy and precision.
CO5 Establish quality assurance principles and practices to ensure the accuracy and reliability of
laboratory information.
Hours Objectives
I Introduction to Clinical Laboratory Science: Basic laboratory 12 CO1
principles - Code of conduct for medical laboratory personnel -
Organization of clinical laboratory and role of medical laboratory
technician - Safety measures. Assessment of a patient and brief history of
collection. Maintenance of Hygiene & Infection Control Practices.
II Specimen collection and processing - Blood, urine, stool, sputum CSF, 12 CO2
amniotic fluid and bile. Separation of serum and plasma, Handling of
specimens for testing, preservation of specimens, transport of specimens
and factors affecting the clinical results.
III Introduction to histopathology-Methods of examination of tissues and 12 CO3
cells, Fixation of tissues: Classification and properties of fixatives.
Tissue processing - Collection of specimens, Labeling and fixation,
Dehydration, Clearing, Impregnation, Embedding - Paraffin block
making, Section Cutting, Microtomes – types and mounting of sections.
IV Introduction to Haematology- Laboratory methods used in the 12 CO4
investigation of coagulation disorders - coagulation tests , Routine
coagulation tests, (prothrombin time , plasma
recalcificationtime,partialthromboplastin time , activated partial
thromboplastin time, thrombin time), Laboratory diagnosis of bleeding
51
disorders. Estimation of fibrinogen, Assay of coagulation factors.

V Quality Standards in Health Laboratories – Development and 12 CO5
implementation of standards, Accreditation Boards –NABL, ISO, CAP,
COLA, Performing quality assessment - pre-analytical, analytical, and
post-analytical phases of testing.
Total 60
Course Outcomes
Outcomes
CO1 Describe characteristics of laboratory organizations and demonstrate PO3, PO11
professionalism by displaying professional conduct, model ethical behavior
and operate as a vital member of the medical lab team.
Practice safety or infection control procedures in the clinical laboratory,
properly use safety equipment and maintain a clean, safe work environment.
CO2 Accurately collect specimens for various purposes. Determine appropriate PO5, PO6,
tests based on test request, Maintain standard and transmission-based PO11
precautions, Engage in the scientific process by understanding the
principles and practices of clinical study design, implementation, and
dissemination of results.
CO3 Identify the basic structure of cells, tissues and organs and describe their PO6, PO8,
contribution to normal function. Interpret light and electron microscopic PO9, PO11
histological images and identify the tissue source and structures. Relate and
recognize the histological appearance of affected tissues to the underlying
pathology.
CO4 Recognize the pathologies behind benign and malignant disorders of PO5, PO6,
erythrocytes, leucocytes, thrombocytes and familiar with the diagnosis, PO9, PO11
evaluation, and management of hematologic malignancies.
CO5 Interpret, implement, and complying with laws, regulations and accrediting PO1,PO10
standards and guidelines of relevant governmental and non-governmental
agencies.
Text Books
1. Mukharji,K.L. (2000).Medical Laboratory Techniques, Vol - I, II & III, 5 th Edition. Tata
McGrawHill, Delhi.
2. Ochei,A., Kolhatkar.A. (2000).Medical Laboratory Science: Theory and Practice, McGraw Hill
Education.
3 RamnikSood (2015).Concise Book of Medical Laboratory Technology:Methods and
Interpretation, 2ndEdition, Jaypee Brothers Medical Publishers, NewDelhi.
4. S. Ramakrishnan, KN Sulochana(2012). Manual of Medical Laboratory Techniques,Jaypee
Brothers Medical Publishers Pvt. Ltd
5. TalibV.H. (2019).Handbook Medical Laboratory Technology, 2ndEdition, Directorate of health
services, Government of India.
References Books
1 Rutherford, B.H. Gradwohl , A.C. Sonnenwirth L. Jarett. Gradwohls. (2000). Clinical Laboratory
Methods and Diagnosis, Vol-I, 8th edition, Mosby.
52
2 Baker, F.J., Silverton, R.E., and Pallister,.J. (1998). An Introduction to Medical Laboratory
Technology, 7thEdition, CBS Publishers and Distributors Pvt. Ltd.
3 Godkar (2021).Textbook of Medical Laboratory Technology, 3rdEdition,Bhalani Publishing
House.
4 M.N.Chatterjee and RanaShinde.(2008). Textbook of Medical Biochemistry, 7 thEdition, Jaypee
Brothers Medical Publishers Pvt. Limited.
5 James G Cappucino. and Natalie Sherman. (2016). Microbiology – A laboratory manual.(5 th
Edition).The Benjamin publishing company. New York.
Web Resources
1 https://www.jaypeedigital.com › book
2 https://www.pdfdrive.com › wintrobes-clinical-hematology
3 https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpet.5
4 https://vlab.amrita.edu/index.php?sub=3&brch=272
5 https://nptel.ac.in/courses/102105087
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
(K2)
(K3) Explain
Analyze (K4)
Create (K6)
Presentations

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10
CO1 M S
CO2 M S S
CO3 S S S S
CO4 M S S S
53
CO5 M M
Subject Subject Name Category L T P S Cred Inst. Marks

Code its Hours
CIA Exter Total
nal
ORGANIC SKILL Y - - - 1 1 25 75 100
FARMING & ENHANCEM
BIOFERTILI ENT
SER COURSE –
TECHNOLO SEC -4
GY (ENTREPRE
NEURIAL
SKILL)
Learning Objectives
CO1 Impart knowledge about the significance of organic farming and strategies to increase the
yield to conserve environment.
CO2 To encourage organic farming in urban areas.
CO3 Comprehensive knowledge about bacterial biofertilizers, its advantages and future
perspective.
CO4 Structure and characteristic featuresofCyanobacterial and fungal biofertilizer
CO5 Develop the knowledge and skill to produce, analyze the quality of packaging, storage and
assess the shelf life and bioefficacy of biofertilizers.
Hours Objectives
I Principle of organic farming: principles of health, fairness, ecological 6 CO1
balance, and care.Environmental benefits of organic farming:
sustainability- reduces non-renewable energy by decreasing
agrochemical need. Biodiversity-crop rotation, inter-cropping.
Ecological services – biological control, soil formation and nutrient
cycling.
II Organic farming for urban space; Create a Sustainable Organic 6 CO2
Garden (Backyard- Square Foot Gardening, Small Space Gardening,
Mini Farming) Composting, Vermicomposting
III Biofertilizers: Introduction, advantages and future perspective. 6 CO3
Structure and characteristic features of bacterial biofertilizers-
Azospirillum, Azotobacter, Bacillus, Pseudomonas, Rhizobium and
Frankia
IV Structure and characteristic features ofCyanobacterialbiofertilizers- 6 CO4
Anabaena, Nostoc ;Structure and characteristic features
offungalbiofertilizers- AM mycorrhiza
54
V Production of Rhizobium, Azotobacter, Anabena;Biofertilizers - 6 CO5

Storage, shelf life, quality control and marketing
Total 30
Course Outcomes
Outcomes
CO1 Become an Entrepreneur with wide knowledge about farming and PO1, PO2, PO7,
sustainable resources. PO8, PO10
CO2 Implement organic farming in urban areas with knowledge on PO1, PO5, PO10
compost.
CO3 Gain knowledge about the bacterial biofertilizers and its PO1, PO5, PO7,
advantages PO8, PO10
CO4 Understand the significance about Cyanobacterial and fungal PO1, PO5, PO7,
biofertilizers PO8, PO10
CO5 Understand and implement the use of bio fertilizers. PO1, PO5, PO7,
PO8, PO10
Text Books
1. A.K. Sharma (2006). Hand book of Organic Farming

2. A.C.Gaur (2017). Hand book of Organic Farming and Biofertilizers
3. N.S. Subbarao (2017). Bio-fertilizers in Agriculture and Forestry (4th Edition) Med tech
publisher
4. SubbaRao, N. S. (2002). Soil Microbiology. Soil Microorganisms and Plant Growth. (4 th
Edition), Oxford & IBH Publishing Co. Pvt. Ltd., New Delhi.
5. Dubey, R. C. (2008). A Textbook of Biotechnology. S. Chand & Co., New Delhi.
References Books
1 Masanobu Fukuoka, Frances Moore Lappe Wendell Berry (2009). The One-Straw
Revolution: An Introduction to Natural Farming, 1st edition, YRB Classics.
2 SujitChakrabarty(2018). Organic Home Gardening Made Easy, 1st Edition,
3 Singh and Purohit (2008). Biofertilizer technology. Agrobios, India.
4 Bansal M (2019). Basics of Organic Farming CBS Publisher.
5 Hurst, C.J., Crawford R.L., Garland J.L., Lipson D.A., Mills A.L. and StetzenbachL.D.
(2007). Manual of Environmental Microbiology. (3rd Edition). American Society for
Microbiology.
Web Resources
1.https://agritech.tnau.ac.in/org_farm/orgfarm_introduction.html
2.https://www.fao.org/organicag/oa-faq/oa-faq6/en/
3.https://www.india.gov.in/topics/agriculture/organic-farming
4. https://agriculture.nagaland.gov.in/bio-fertilizer/
5. https://vlab.amrita.edu/index.php?sub=3&brch=272
Internal Continuous Internal Assessment Test 25 Marks
Evaluation Assignments
Seminars
55

External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO/PO PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
CO1 S S S S S
CO2 S S S
CO3 S S S S S
CO4 S S S S S
CO5 S S S S S
56
Subject Subject Name Category L T P S Credit Inst. Marks

Code s Hour CI Exter Tota
s A nal l
AQUACULT Skill Y - - - 2 2 25 75 100
Enhancem
URE
ent Course
-5
Learning Objectives
CO1 Provide a deeper knowledge in aquaculture systems and methods.
CO2 Explain the significance and functions of design, types and construction of aquaculture
ponds.
CO3 Demonstrate the biological characteristics of various aquaculture species.
CO4 Discuss the methods involved in post stocking management.
CO5 Illustrate major cultivatable species for aquaculture.
Hours Objecti
ves
I Aquaculture Systems and Methods - Scope and definition. 6 CO1
Traditional, extensive, semi - intensive and intensive culture.
Monoculture, polyculture, composite culture, mixed culture, mono-
sex culture, cage culture, pen culture, raft culture, race way culture.
II Aquaculture Engineering - Design and construction of pond, lay-out 6 CO2
and design of aquaculture farm, construction, water intake system,
drainage system - aeration and aerators. Ponds - Types of ponds.
III Selection of Species - Biological characteristics of aquaculture 6 CO3
species; economic and market considerations; seed resources,
collection and transportation. Pre-Stocking Management-Sun drying,
ploughing / tilling, desilting, liming and fertilization, eradication of
weed fishes. Stocking - Acclimatization of seed and release - species
combinations - stocking density and ratio.
IV Post Stocking Management - Water and soil quality parameters 6 CO4
required for optimum production, control of aquatic weeds and
aquatic insects, algal blooms and microorganisms. Food conversion
ratio (FCR). Growth - Measurement of growth, length - weight
relationship.
V Major cultivable species for aquaculture –Culture of Indian Major 6 CO5
Carps. Culture of Giant fresh water prawn,
57
Macrobrachiumrosenbergii - seed collection formation sources.

Hatchery management. Culture of tiger shrimp, Penaeusmonodon
and LitopenaeusVannamei. Culture of pearl oysters. Culture of sea
weeds. Methods of Crab culture. Culture of ornamental fishes.
Culture of Molluscs.
Total 30
Course Outcomes
Outcomes
CO1 Analyze the significance and importance of aquaculture PO4, PO5,
PO7,PO9
CO2 Illustrate the types and construction of aquaculture ponds PO4, PO7,PO9
CO3 Analyze the biological characteristics of species and choose the PO5, PO7,PO9
best species for aquaculture.
CO4 Follow methods involved for optimal growth of aquaculture PO7,PO9
species
CO5 Summarize major species suitable for aquaculture in a particular PO5, PO6,
PO7,PO9
environment
Text Books
1. Santhanam, R. Velayutham, P. Jegatheesan, G. A (2019).Manual of Freshwater Ecology:
An Aspect of Fishery Environment. Daya Publishing House, New Delhi.
2. Stickney, R.R. (2016). Aquaculture: An Introductory Text. 3rd Edition. Centre for
Agriculture and Bioscience International Publishing.
3. Ackefors H., Huner J and Konikoff M. (2009). Introduction to the General Principles of
Aquaculture. CRC Press.
4. Mushlisin Z. A. (2012). Aquaculture. In Tech.
5. Akpaniteaku R.C. (2018).Basic Handbook of Fisheries and Aquaculture.AkiNik
Publications.
References Books
1. Arumugam N. (2014). Aquaculture. Saras Publication.
2. Pillay T. V. R. and Kutty M.N. (2005). Aquaculture : Principles and Practices. 2 ndEdition.
Wiley India Pvt. Ltd.
3. Tripathi S. D., Lakra W.S. and Chadha N.K. (2018). Aquaculture in India. Narendra
Publishing House.
4. Rath R.K.(2011). Fresh Water Aquaculture. 3rdEdition. Scientific Publishers.
5. Lucas J. S., Southgate P.C. and Tucker C.S. (2019). Aquaculture: Farming Aquatic
Animals and Plants. Wiley Blackwell.
Web Resources
1. Aquaculture: Types, Benefits and Importance (Fish Farming) - Conserve Energy Future
(conserve-energy-future.com)
2. Fisheries Department - Tamil Nadu (tn.gov.in)
3. Aquaculture - Google Books
58
4. aquaculture | Definition, Industry, Farming, Benefits, Types, Facts, & Methods | Britannica
5. Fisheries & Aquaculture (investindia.gov.in)
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO1 S S M S M S M
CO2 S M M S M S L
CO3 M S M S M S L
CO4 M M M S M S L
CO5 M S S S M S L
59
SEMESTER---IV
Code s Hour
s CI Exter Total
A nal
IMMUNOLOGY CORE Y - - - 3 4 25 75 100
AND COURS
IMMUNOTECH E – VII
NOLOGY
Course Objectives
CO1 To gain knowledge about immune system, organs of immunity and cells involved.
CO2 To distinguish the types of antigens and antibodies; their properties.
CO3 To provide in-depth knowledge on immuno-techniques.
CO4 To discuss the role of MHC system in transplantation; functions of Tumor specific
antigens.
CO5 To impart knowledge on immunological disorders.

Hours Objectives
I Organs and Cells in Immune System and Immune 12 CO1
Response:Primary lymphoid organs, secondary lymphoid organs,
and lymphoid tissues; T – cell and B –cell membrane bound
receptors – apoptosis; T - cell processing, presentation and
regulation; T –cell subpopulation, properties, functions and T – cell
suppression; Physiology of immune response- innate, humoral and
cell mediated immunity; Immunohematology.
II Antigen and Antibody:Antigens - Properties of haptens, epitopes, 12 CO2
adjuvants, and cross reactivity; Antibodies- structure, properties,
classes; Antigen and Antibody Reactions: precipitation,
agglutination, complement fixation, opsonization, neutralization;
Vaccines – active and passive immunization; Classification of
vaccines; Other approaches to new vaccines; Types of vaccine -
antibacterial, antiviral; Vaccination schedule.
III Immunoassay and Immunotechniques - Preparation and 12 CO3
standardization of bacterial antigens; Raising of monoclonal and
polyclonal antibodies; Purification of antibodies. Immunotechniques
- RIA, RAST, ELISA, Immuno fluorescence techniques and Flow
60
cytometry
IV Transplantation and TumorImmunology - MHC Antigens - structure 12 CO4
and function; HLA system - Regulation and response to immune
system; Transplantation immunology - tissue transplantation and
grafting; Mechanism of graft acceptance and rejection; HLA typing;
Tumor specific antigens; Immune response to tumors; Immune
diagnosis; cancer immune therapy.
V Immunological disorders and diseases - Hypersensitivity reactions 12 CO5
(Type I, II, III and IV); acquired immunodeficiency syndrome; Auto
immune disorders and diseases: organ specific and non-organ
specific.
Total 60
Course Outcomes

Outcome
s
CO1 Assess the fundamental concepts of immunity, contributions of PO1, PO4, PO6,
the organs and cells in immune responses. PO9,
CO2 Investigate the structures of Ag and Ab; Immunization. PO1, PO4, PO5,
PO9
CO3 Justify the Immunoassay and Immunotechniques. PO1, PO4, PO5,
PO7
CO4 Explain about the immunologic processes governing graft PO1, PO3, PO4,
rejection and therapeutic modalities for immunosuppression in PO5, PO9
transplantation
CO5 Analyze the overreaction by our immune system leading to PO1, PO4, PO5,
hypersensitive conditions and its consequences. PO6
Text Books
1. Richard Coico, Geoffrey Sunshine, Eli Benjamini. (2003). Immunology – A Short

Course. 5thEdition., Wiley-Blackwell, New York.
2. Judith A.Owen, Jenni Punt, Sharon A. Stranford, Janis Kuby. (2013). Immunology,
7thEdition., W. H. Freeman and Company, New York.
3. Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai. (2021). Cellular and Molecular
Immunology, 10thEdition.,Elsevier.
4. Robert R. Rich, Thomas A. Fleisher, William T. Shearer, Harry Schroeder, Anthony J.
Frew, Cornelia M. Weyand. (2018).Clinical Immunology: Principles and Practice, 5th
Edition. Elsevier.
61
5. Pravash Sen. Gupta. (2003). Clinical Immunology. Oxford University Press.
References Books
1 Janeway Travers. (1997).Immunobiology- the immune system in health and disease.

Current Biology Ltd. London, New York. 3rd Edition.
2 Peter J. Delves, Seamus Martin, Dennis R. Burton, Ivan M. Roitt. (2006). Roitt’s
Essential Immunology, 11thEdition.,Wiley-Blackwell.
3 William R Clark. (1991). The Experimental Foundations of Modern Immunology.

3rdEdition. John Wiley and Sons Inc. New York.
4 Frank C. Hay, Olwyn M. R. Westwood. (2002). Practical Immunology, 4thEdition.,
Wiley-Blackwell.
5 Noel R. Rose, Herman Friedman, John L. Fahey. (1986). Manual of Clinical Laboratory
Immunology. ASM.3rd Edition.
Web Resources
1 https://www.ncbi.nlm.nih.gov/books/NBK279395/
2 https://med.stanford.edu/immunol/phd-program/ebook.html
3 https://ocw.mit.edu/courses/hst-176-cellular-and-molecular-immunology-fall-2005/
pages/lecture-notes/
4 Immunology Overview - Medical Microbiology - NCBI Bookshelf (nih.gov)
5 Immunology - an overview | ScienceDirect Topics
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehen
overview
d (K2)
(K3) Explain
62
Analyze Problem-solving questions, Finish a procedure in many steps, Differentiate

(K4) between various ideas, Map knowledge
Evaluate
(K5)
Create (K6)
Presentations
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9

CO1 S M S M
CO2 S M M M
CO3 S S S S
CO4 S M S S M
CO5 S S M M

Code dits Hou CIA Exter Total
rs nal
IMMUNOLOG CORE - - Y - 4 5 40 60 100
Y AND COURSE –
IMMUNOTECH VIII-
NOLOGY PRACTICA
L
IV
Course Objectives
CO1 To gain hands-on knowledge to identify Blood group and typing.
CO2 To acquire adequate skill to perform latex agglutination reactions.
CO3 To analyze precipitation reactions in gels.
CO4 To investigate the antigen & antibody reactions in electrophoresis.
CO5 To familiarize with Separation of Lymphocytes.
Hours Objectives
I Identification of blood group and typing. 12 CO1
Coomb’s test. TPHA
II T cell identification (Demonstration) 12 CO2
Latex Agglutination reactions- RF, ASO, CRP
III Ouchterlony’s Double Diffusion Method (antigen pattern). 12 CO3
Single Radial Immuno Diffusion Method.
63
IV Electrophoresis - Serum, Counter and Immuno. 12 CO4

V Separation of Lymphocytes by gradient centrifugation method. 12 CO5
ELISA: Hepatitis/ HIV
Total 60
Course Outcomes

Outcome
s
CO1 Assess the blood groups and types PO1,PO5, PO6, PO7, PO8
CO2 Competently perform serological diagnostic tests such as PO4, PO5, PO6, PO7, PO8
RF, ASO, CRP
CO3 Illustrate the antigen antibody reactions in gel. PO5, PO6, PO7, PO8, PO9
CO4 Compare & contrast antigens and antibodies in PO5, PO6, PO7, PO8, PO9
electrophoresis
CO5 Examine the concept of ELISA. PO5, PO6, PO7, PO8, PO9
Text Books
1. Talwar. (2006). Hand Book of Practical and Clinical Immunology, Vol. I, 2nd edition, CBS.
2. Asim Kumar Roy. (2019). Immunology Theory and Practical, Kalyani Publications.
3. Richard Coico, Geoffrey Sunshine, Eli Benjamini. (2003). Immunology – A Short Course.
5thEdition., Wiley-Blackwell, New York.
4. Judith A.Owen, Jenni Punt, Sharon A. Stranford, Janis Kuby. (2013). Immunology,
7thEdition., W. H. Freeman and Company, New York.
5. Pravash Sen. Gupta. (2003). Clinical Immunology. Oxford University Press.
References Books
1 Frank C. Hay, Olwyn M. R. Westwood. (2008).Practical Immunology, 4th Edition, Wiley-
Blackwell.
2 Wilmore Webley. (2016). Immunology Lab Manual, LAD Custom Publishing.
3 Rose. (1992). Manual of Clinical Lab Immunology, ASM.
4 Janeway Travers. (1997).Immunobiology- the immune system in health and disease.
Current Biology Ltd. London, New York. 3rd Edition.
5 Peter J. Delves, Seamus Martin, Dennis R. Burton, Ivan M. Roitt. (2006). Roitt’s Essential
Immunology, 11thEdition.,Wiley-Blackwell.
Web Resources
1 https://www.researchgate.net/publication/275045725_Practical_Immunology-
_A_Laboratory_Manual
2 https://www.urmc.rochester.edu/MediaLibraries/URMCMedia/labs/frelinger-lab/
documents/Immunology-Lab-Manual.pdf
3 https://webstor.srmist.edu.in/web_assets/downloads/2021/18BTC106J-lab-manual.pdf
64
4 Immunology Overview - Medical Microbiology - NCBI Bookshelf (nih.gov)

5 Immunology - an overview | ScienceDirect Topics
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations

CO1 M S S S S
CO2 S M M S S
CO3 M S S S M
CO4 M M S S M
CO5 M M S S M
65

Code dits Hours CIA Exter Total
nal
FOOD ELECTIVE Y - - - 2 3 25 75 100
PROCESSING GENERIC/D
TECHNOLOG ISCIPLINE
Y SPECIFIC
ELECTIVE -
IV
Learning Objectives
CO1 To provide knowledge on objectives of food preservation.
CO2 To explain the freshness criteria and quality assessment of meat and fish.
CO3 To outline the methods of milk processing and fermented milk products.
CO4 To explain the importance of fat and oil processing.
CO5 To discuss the methods of microbiological examination of foods.
Hours Objectives
I Introduction to food preservation –objectives and techniques of food 12 CO1
preservation. Preservation: principles of high temperature, low
temperature, radiation, chemical preservatives and bio preservatives.
II Freshness criteria and quality assessment of meat and fish –spoilage 12 CO2
and methods of preservation. Production of byproducts after processing
waste and their utilization. Role of packaging material, types of
packaging material.
III Composition of milk; assessment of milk, thermal processing of fluid 12 CO3
milk-pasteurization (LTH, HTST&UHT techniques). Fermented milk
products-cheese, Butter milk, Yogurt, Kumis, Kefir and Acidophilus
milk. Hygiene and sanitation requirement in food processing and
fermentation industries.
IV Importance of fats and oils in Food-Extraction of fats and Oils- 12 CO4
Rendering, pressing, solvent extraction, pressing of oil- degumming,
refining, bleaching, deodorization, fractionation, pyrolysis of fats,
toxicity of frying oil.
V Methods for the microbiological examination of foods. Food borne 12 CO5
illness and diseases. Microbial cultures for food fermentation. Indian
Factories Act on safety, HACCP, Safety from adulteration of food.
Total 60
Course Outcomes
Outcomes
CO1 Assess the fundamental concepts of food preservation. PO1, PO3, PO5,PO6,
66
PO8
CO2 Investigate the quality assessment of meat and fish. PO1, PO5, PO6,
PO7, PO8
CO3 Design the processing of milk and milk quality assessment. PO1, PO5, PO6,
PO7, PO8
CO4 Explain about the importance of fats and oils. PO1, PO4, PO6,
PO7, PO8
CO5 Plan the food safety and adulteration detection. PO3, PO4, PO6,
PO7, PO8
Text Books
1. Avantina Sharma. (2006). Text Book of Food Science and Technology, International Book
Distributing Co, Lucknow, UP.
2. Sivasankar. (2005). Food Processing and Preservation, 3rd Edition.,Prentice hall of India Pvt
Ltd, NewDelhi.
3 Ramaswamy H &Marcotte M. (2006). Food Processing: Principles & Applications. Taylor &
Francis.
4 NIIR Board of Food and Technologist. (2005). Modern Technology of Food Processing and
Agrobased industries, National Institute of Industrial Research, Delhi.
5 Adams M.R. and Moss M. O (2007).Food Microbiology.New Age International.
Reference Books
1 Fellos PJ. (2005). Food Processing Technology: Principle &Practice 2ndEdition. CRC.
2 Peter Zeuthen and Leif Bogh-Sorenson. (2005). Food Preservation Techniques,
WoodlandPublishing Ltd, Cambridge, England.1
3 Gustavo V. Barbosa-Canovas, Maria S. Tapia, M. Pilar Cano. (2004). Novel Food Processing
Technologies, CRC.
4 SumanBhatti, Uma Varma. (1995). Fruit and vegetable processing organizations and
institutions, 1st Edition., CBS Publishing, New Delhi.
5 MirdulaMirajkar, SreelathaMenon. (2002). Food Science and Processing Technology Vol-
2,Commercial processing and packaging, Kanishka publishers, New Delhi.
Web Resources
1 https://sites.google.com/a/uasd.in/ecourse/food-processing-technology
3 https://engineeringinterviewquestions.com/biology-notes-on-food-adulteration/
4 food processing | Definition, Purpose, Examples, & Facts | Britannica
5 Food Processing Technology | Food News & Views Updated Daily (foodprocessing-
technology.com)
67

25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations

CO1 M M S M S
CO2 M S M S S
CO3 M S M S S
CO4 M S S S S
CO5 M M M S S
68
Subjec Subject Name Category L T P S Credi Inst. Marks

t ts Hou CI Extern Total
Code rs A al
Vaccine Skill Y - - - 2 2 25 75 100

Enhancem
Technology ent Course
SEC -6
Course Objectives
CO1 To provide knowledge on the basics of immunization and induction of immunity.

CO2 To learn the types of vaccines, its immunological effects and regulatory guidelines.
CO3 To learn the role of rDNA in vaccine technology.
CO4 To provide the knowledge on conventional to recent technology of vaccine production
CO5 To learn about ethical issues and regulations in vaccine production and clinical trials
Unit Details No.of Course Objectives
Hours
I History of vaccination, Active and passive immunization; 3hrs CO1
requirements for induction of immunity, Epitopes, linear and
conformational epitopes, characterization and location of
APC, MHC and immunogenicity,
II Viral/bacterial/parasite vaccine differences, methods of 6 CO2
vaccine preparation – Live, killed, attenuated, sub unit
vaccines;Licensed vaccines, Viral Vaccine - Poliovirus
vaccine-inactivated & Live, Rabies vaccines, Hepatitis A &
B vaccines, Bacterial Vaccine - Anthrax vaccines, Cholera
vaccines, Diphtheria toxoid, Parasitic vaccine - Malaria
Vaccine.
III Vaccine technology- Role and properties of adjuvants, 5 CO3
recombinant DNA and protein-based vaccines, plant-based
vaccines, reverse vaccinology; Peptide vaccines, conjugate
vaccines. Recent advances in Malaria, Tuberculosis, HIV.
IV Fundamental research to rational vaccine design. Antigen 5 CO4
identification and delivery, T-Cell expression cloning for
identification of vaccine targets for intracellular
pathogens,Rationale vaccine design based on clinical
requirements: Scope of future vaccine strategies.
69
V Vaccine additives and manufacturing residuals, Regulation 5 CO5

and testing of vaccines, Regulation of vaccines in
developing countries, Quality control and regulations in
vaccine research, Animal testing, Rational design to clinical
trials, Large scale production, Commercialization. Vaccine
safety ethics and Legal issues.
Total 24
Course Outcomes
Outcomes
CO1 Explain the significance of critical antigens, immunogens and PO1,PO10
adjuvants in developing effective vaccines.
CO2 Understand the types of vaccines. PO5
CO3 Construct vaccine applying rDNA technology. PO7,PO10
CO4 Formulate the strategies for developing an innovative vaccine PO9,PO10
technology with different mode of vaccine delivery.
CO5 Evaluate the regulatory issues and guidelines for the management PO3,PO5
of vaccine production.
Text Books
1. Ronald W. Ellis.(2001). New Vaccine Technologies.Landes Bioscience.
2. Cheryl Barton. (2009). Advances in Vaccine Technology and Delivery.Espicom Business
Intelligence.
3 Male, David. Ed. (2007). Immunology. 7th Edition. Mosby Publication.
4 Kuby, RA Goldsby, Thomas J. Kindt, Barbara, A. Osborne. (2002). Immunology. 6 th Edition,
Freeman.
5 Brostoff J, Seaddin JK, Male D, Roitt IM. (2002). Clinical Immunology. 6 th Edition, Gower
Medical Publishing.
References Books
1 Stanley A. Plotkin, Walter Orenstein& Paul A. Offit.(2013). Vaccines, 6 th Edition. BMA
Medical Book Awards Highly Commended in Public Health. Elsevier Publication.
2 Coico, R. etal. (2003). Immunology: A Short Course. 5th Edition, Wiley – Liss.
3 Parham, Peter.(2005). The Immune System. 2nd Edition, Garland Science.
4 Abbas, A.K. etal. (2007). The Cellular and Molecular Immunology. 6th Edition, Sanders /
Elsevier.
5 Weir, D.M. and Stewart, John (2000). Immunology. 8th Edition, Churchill Pvt. Ltd.
Web Resources
1 https://www.slideshare.net/adammbbs/pathogenesis-3-rd-internal-updated-43458567
70
2 https://www.bio.fiocruz.br/en/images/stories/pdfs/mpti/2013/selecao/vaccine-
processtechnology.pdf
3 https://www.dcvmn.org/IMG/pdf/ge_healthcare_dcvmn_introduction_to_pd_for_vaccine_
production_29256323aa_10mar2017.pdf
4 https://www.sciencedirect.com/science/article/pii/B9780128021743000059
5 https://www.researchgate.net/publication/313470959_Vaccine_Scaleup_and_Manufacturing

Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
Comprehend
(K2) overview
Evaluate (K5)
Presentations
CO1 M M
CO2 S
CO3 M M
CO4 L M
CO5 L M
71

Code Hours CIA External Total
APICULTURE SKILL Y - - - 2 2 25 75 100

ENHANCEMENT
COURSE- SEC – 7
Course Objectives
CO1 To understand the biology of honey bees.
CO2 To study on honey bee colony establishment.
CO3 To develop knowledge on honey extraction.
CO4 To understand the diseases of honey bees and their control.
CO5 To gain information on financial assistance and funding agencies for bee keeping industry.
Hours Objectives
I Biology of Bees: Honeybee – Systematic position – Species of 6 CO1

Honey bees – Life history of Honey bee – behaviour – swarming –
Pheromone.
II Social life in Bees:Bee colony – Castes – natural colonies and their 6 CO2
yield – Types of bee hives – Structure – location, care and
management.
III Bee Rearing:Apiary – Care and Management – Artificial bee hives 6 CO3
– types – construction of spaceframes – Selection of sites –
Handling – Maintenance – Instruments employed in Apiary –
Extraction instruments.
IV Bee Economy: Honey – Composition – uses – Bee wax and its uses 6 CO4
– yield in national and international market – Diseases of honey
bees and their control methods. Economics of bee culture.
V Entrepreneurship: venture – Preparing proposals for financial 6 CO5
assistance and funding agencies – Bee Keeping Industry – Recent
Efforts, Modern Methods in employing artificial Beehives for cross
pollination in horticultural gardens.
Total 30
Course Outcomes
Outcomes
CO1 Understand the systematic position and life history of honey bee. PO1, PO2, PO10
CO2 Reveal the different stages and types of bees and discuss about the PO1, PO2, PO4, PO5
care and management of apiculture.
CO3 Describe the practice of bee rearing process and analyze PO2,PO4, PO5, PO10,
72
instruments employed in apiary. PO11

CO4 Compare and contrast the composition of honey and bee wax and PO4, PO5, PO7, PO8,
interpret the yield in National and International markets. PO10
CO5 Clarify the proposal for financial assistance and funding agencies PO5, PO8, PO9, PO10,
and reveal the modern methods employed in artificial bee hives. PO11
Text Books
1. Dewey M. Caron. (2013). Honey Bee Biology and Beekeeping. Revised Edition. Wicwas Press,
Kalamazoo. ISBN 10: 1878075292
2. R. A. Morse. (1993). Rearing queen honey bees. Wicwas press, NY. ISBN-10 : 1878075055
3. Ted Hooper. (2010). Guide to Bees & Honey: The World's Best Selling Guide to Beekeeping.
Northern Bee Books. Oxford. ISBN 10: 1904846513
4. Jayashree K. V., Tharadevi C.S. and Arumugam N. (2014) Apiculture.Saras Publication
5. Raj H. (2020).Vinesh Text Book of Apiculture. S. Vinesh and Co.
References Books
1 Dewey M. Caron. (2020). The Complete Bee Handbook: History, Recipes, Beekeeping Basics,
and More,Rockridge Press. ISBN-10 : 1646119878
2 Joachim Petterson. (2016). Beekeeping: A Handbook on Honey, Hives & Helping the Bees,
Weldon Owen.
3 Eva Crane. (1999). The World History of Beekeeping and Honey Hunting. Routledge. India.ISBN-
10 : 0415924677
4 Pagar B. S. (2016). Textbook Of Apiculture. SahityaSagar.
5 Sehgal P.K. (2018). Text Book of Sericulture, Apiculture and Entomology.Kalayani.
Web Resources
Bee Keeping Basics. Retrieved from:https://denton.agrilife.org/files/2013/08/beekeeping-
1
basics.pdf
Beekeeping as an Entrepreneurship, Retrieved from: https://lupinepublishers.com/agriculture-
2
journal/pdf/CIACR.MS.ID.000270.pdf
Raising Bumble Bees at Home: A Guide to Getting Started. Retrieved from:
3
https://www.ars.usda.gov/ARSUserFiles/20800500/BumbleBeeRearingGuide.pdf
4 Apiculture – Biology for Everybody (homeomagnet.com)
5 Apiculture: Introduction to Apiculture (iasri.res.in)
25 Marks
Evaluation Seminars
73
Evaluation
Total 100 Marks
Understand/
(K2)
(K3) Explain
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations
CO1 S S S
CO2 S S S S
CO3 S S M S S
CO4 S M S S M
CO5 S S S S S
74
V- SEMESTER
Code s Hour CI Exter Tota
s A nal l
BACTERIOLO Core Y - - - 4 5 25 75 100
GY AND Course
IX
MYCOLOGY
Course Objectives
CO1 Understand the role of normal flora and pathogenic microbes of various diseases and
clinical microbiological techniques.
CO2 Basic knowledge about Gram positive pathogenic bacteria and their epidemiology
CO3 Acquire knowledge about Gram negative pathogenic bacteria and nosocomial infections
CO4 Comprehensive knowledge about medically important, its classification and its significance
CO5 Gain knowledge about the general characteristics and mode of action of various
antibacterial agents
Hours Objectives
I History, Classification of Medically Important Microbes, Koch’s, 12 CO1
and River’s postulates-A brief account on the normal microbial flora
of the healthy human body – Host-pathogen interactions: Definitions
of infection, invasion, primary and opportunistic pathogens,
pathogenicity, virulence, toxigenicity, carriers, endemic, epidemic,
pandemic diseases and epidemiology – putative virulence factors of
human pathogens –infectious disease cycle. Collection and transport
of clinical specimens for bacterial and fungal infections.
II Medically important Gram Positive infections - Causative agent, 12 CO2
clinical symptoms, pathogenesis, mode of transmission, prevention
and treatment of the following bacterial diseases (a) Streptococcal
infections (Streptococcus pyogenes, Streptococcus faecalis), (b)
Staphylococcal infections (Staphylococcus aureus), (c) Tetanus
(Clostridium tetani)(d) Diphtheria (Corynebacteriumdiphtheriae) (e)
Anthrax (Bacillus anthracis) (f) Tuberculosis (Mycobacterium
tuberculosis), (g) Leprosy (Mycobacterium leprae).
III Medically important Gram-Negative infections - Causative agent, 12 CO3
clinical symptoms, pathogenesis, mode of transmission, prevention,
and treatment of the following bacterial diseases (a) Meningitis
(Streptococcus pneumoniae, Neisseria meningitidis) (b) typhoid
(Salmonella typhi, Salmonella paratyphi) (c) cholera (Vibrio
75
cholerae) (d) bacillary dysentery (Shigelladysenteriae); Sexually

Transmitted disease (syphilis–Treponemapallidum.Gonorrhoea -
Neisseria gonorrhoeae); Nosocomial infections – definition,
importance, and their control (Pseudomonas aeruginosa).
IV Medically important Fungi - Classification of medically important 12 CO4
fungi; Superficial mycoses: PityriasisVersicolor; TineaNigra;
Piedra. Cutaneous mycoses: Microsporumspps.,Trichophytonspps.,
and Epidermophytonfloccosum. Subcutaneous mycoses:
Chromoblastomycosis; Sporotrichosis; Systemic Mycoses
- Blastomycosis; Histoplasmosis; Opportunistic Infections -
Candidiasis; Cryptococcosis; Zygomycosis; Mycotoxins: Aflatoxin
V Antimicrobial agents -General characteristics and mode of action of 12 CO5
Antibacterial agents: Modes of action with an example for each:
Inhibitor of nucleic acid synthesis; Inhibitor of cell wall synthesis;
Inhibitor of cell membrane function; Inhibitor of protein synthesis;
Inhibitor of metabolism Antifungal agents: Mechanism of action of
Amphotericin B, Griseofulvin.
Total 60
Course Outcomes
Outcomes
CO1 Understand the importance of normal flora of human body and PO1, PO3, PO5,
acquire knowledge on the process of infectious disease. PO7, PO10, PO11
CO2 Explain the various bacterial pathological events during the PO1, PO3, PO5,
progression of an infectious disease, and apply the underlying PO7, PO10, PO11
mechanisms of spread of disease and its control.
CO3 Compile a list of disease-causing bacteria and compare their PO1, PO3, PO5,
modes of infection, symptoms, diagnosis and treatment. PO7, PO10, PO11
CO4 Comprehend human-fungal interaction, which can be applied to PO1, PO3, PO5,
obtain in-depth knowledge on fungal diseases and the PO7, PO10, PO11
mechanism behind the disease process.
CO5 Explain the types of mycoses caused in humans and categorize PO1, PO3, PO4,
the modes of infection, pathogenesis, and treatment with PO5,PO6,
introduction to mycotoxins. PO7,PO9, PO10
Text Books
1 Tom Parker, M. Leslie H. Collier. (1990). Topley&Wilson’s Principles of
Bacteriology, Virology and Immunity,8th Edition. London: Edward Arnold.
2 Greenwood, D., Slack, R.B. and Peutherer, J.F. (2012) Medical Microbiology, 18thEdition.
Churchill Livingstone, London.
3 Finegold, S.M. (2000) Diagnostic Microbiology, 10th Edition. C.V. Mosby Company, St.
76
Louis.
4 Ananthanarayanan, R. and JayaramPanicker C.K. (2020) Text book of Microbiology. Orient
Longman, Hyderabad.
5 JagdishChander (2018). Textbook of Medical Mycology, 4th edition, Jaypeebrothers medical
publishers.
References Books
1 Gerhardt, P., Murray, R.G., Wood, W.A. and Kreig, N.R. (Editions) (1994) Methods for
General and Molecular Bacteriology. ASM Press, Washington, DC.
2 Kevin Kavanagh, (2018). Fungi Biology and Applications 3rd Edition. Wiley Blackwell
publishers.
3 C.J. Alexopoulos, C.W. Mims, M. Blackwell, (2007). Introductory Mycology, 4th edition.
Wiley publishers.
4 A.J. Salle (2007). Fundamental principles of bacteriology, fourth edition, Tata McGraw-Hill
Publications.
5 Christopher C. Kibbler ,RichardBarton,Neil A. R. Gow, Susan Howell,Donna M.
MacCallum, Rohini J. Manuel (2017). Oxford Textbook of Medical Mycology. Oxford
University Press.
Web Resources
1 http://textbookofbacteriology.net/nd
2 https://microbiologysociety.org/members-outreach-resources/links.html
3 http://mycology.cornell.edu/fteach.html
4 https://www.adelaide.edu.au/mycology/
5 https://www.isham.org/mycology-resources/mycological-links
Continuous Internal Assessment Tests 25 Marks
Internal
Evaluation
Assignments
Seminars
Attendance and Class Participitation
Evaluation
Total 100 Marks
Understand /
Comprehend
overview
(K2)
77
Analyse Problem-solving questions, Finish a procedure in many steps, Differentiate

(K4) between various ideas, Map knowledge
Evaluate
(K5)
Presentations

CO1 S S S S M S
CO2 S S S S M S
CO3 S S S S M S
CO4 S S S S M S
CO5 S S M S M S S M
78

s A nal
VIROLOGY AND CORE Y - - - 4 5 25 75 100
PARASITOLOGY COURSE
X
Course Objectives
CO1 To gain knowledge on properties and classification of viruses and collection of relevant clinical
samples for diagnosing viral infections.
CO2 To understand pathogenic microorganisms of viruses and the mechanisms by which they cause
disease in the human body.
CO3 To gain knowledge about reemerging viral infections and develop diagnostic skills, including
the use and interpretation of laboratory test in the diagnosis of infectious diseases.
CO4 Understand the types of parasites causing infections in the intestine.
CO5 To develop skills in the diagnosis of parasitic infections.
Hours Objectives
I General Properties, replication and Classification of viruses (Baltimore 12
classification), Cultivation of viruses- in animals, embryonated eggs CO1
and tissue culture, Virus purification assays - collection and transport

of clinical specimens for viral infections.
II Viral diseases with reference to symptoms, pathogenesis, transmission, 12 CO2
prophylaxis and control – Arboviruses (Flavi virus), Picorna viruses
(Polio virus and Rhinovirus), Hepatitis viruses (HAV, HBV, HCV,
HDV, HEV), Rabies virus, Orthomyoviruses (Influenza virus) and
Paramyxoviruses (Mumps and Measles virus), Pox viruses (Variola,
Vaccinia), Herpes viruses (Herpes simplex, Varicella zoster), Adeno
viruses, Rota viruses and HIV viruses. Oncogenic viruses (Human
Papilloma virus): Introduction, characteristics of transformed cells,
mechanism of viral oncogenesis and clinical manifestations.
III Emerging and reemerging viral infections (SARS, Swine flu, Ebola, 12 CO3
Dengue, Chikungunya- and Corona) – causes, spread and preventive
79
measures. Detection of viruses in clinical specimens – Serological and

Molecular diagnosis of virus infections – Antiviral agents, Interferons
and Viral Vaccines, Immunization schedules.
IV General introduction to Medical Parasitology, Classification of 12 CO4
medically important parasites. Morphology, life cycle, pathogenesis,
clinical features, laboratory diagnosis, prevention and treatment of
diseases caused by the following organisms: Entameobahistolytica,
flagellates (Giardia lamblia, Leishmaniadonovani), Sporozoa-
Plasmodiumspps.
V Introduction to Helminthes, Platyhelminthes – Taenia – Fasciola – 12 CO5
Paragonimus – Schistosomaspps.. Nemathelminthes – Ascaris–
Ankylostoma – Enterobius – Trichuris – Trichinella – Wuchereria –
Dracanculus. Collection, transport and examination of specimen
Laboratory techniques in parasitology Examination of faeces for ova
and cyst by direct wet mount and iodine wet mount, Concentration
methods (Floatation and Sedimentation techniques), Examination of
blood for parasites. Cultivation of parasites.
Total 60
Course Outcomes
Outcomes
CO1 Understand the structure and properties of viruses, cultivation methods and PO5,PO10
diagnosis of viral diseases.
CO2 Knowledge of basic and general concepts of causation of disease by the PO5,PO10
pathogenic microorganisms and various parameters of assessment of their
severity and the methods of diagnosis.
CO3 Insights to treatment options of viral diseases. PO5,PO10
CO4 Knowledge about the importance of protozoans in the intestine. PO5,PO10
CO5 Knowledge of Nematodes as infectious agent PO5,PO10
TEXT BOOKS
1. S., Rajan (2007). Medical microbiology, MJP publisher.
2. Jeyaram Paniker, C.K. (2006). Text Book of Parasitology Jay Pee Brothers, NewDelhi.
3 Arora D.R. and Arora B. (2002). Medical Parasitology, 1 stEdition CBS Publishers &
Distributors, New Delhi.
80
4 Chatterjee (1986). Medical Parasitology. Tata McGraw Hill, Calcutta.

5 Parija S. C. (1996). Text Book of Medical Parasitology.4th edition, Orient Longman, AllIndia
Publishers & Distributors.
References Books
1 Jawetz, E., Melnick, J.L. and Adelberg, E.A. (2000). Review of Medical Microbiology,
19thEdition. Lange Medical Publications, U.S.A.
2 Ananthanarayan, R. and JeyaramPaniker, C.K. (2009). Text Book of Microbiology, 8 thEdition.
Orient Longman, Chennai .
3 Conrat HF, Kimball PC and Levy JA. (1988). Virology. II edition. Prentice Hall,
Englewood Cliff, New Jersey..
4 Topley& Wilsons’s (1990). Principles of Bacteriology, Virology and Immunity, 8 th Edition,
Vol. III Bacterial Diseases, Edward Arnold, London.
5 Finegold, S.M. (2000). Diagnostic Microbiology, 10th Edition. C.V. Mosby Company,St.Louis.
Web Resources
1 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4047123/
2 https://www.ncbi.nlm.nih.gov/pubmed/21722309
3 https://www.sciencedirect.com/science/article/pii/S2211753919300193
4 https://cmr.asm.org/content/30/3/811
5 https://www.nejm.org/doi/full/10.1056/NEJMoa1811400
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
(K2)
(K3) Explain
Presentations
81

CO1 M M
CO2 M M
CO3 M M
CO4 M M
CO5 M M
82
Subject Subject Name Categor L T P S Credit Inst. Marks

Code y s Hour
s CIA Externa Total
l
PRACTICAL V Core Y - - - 4 5 40 60 100
course
XI
Course Objectives
CO1 Learning Objectives

To familiarize students with medical microbiology techniques and technical knowledge on
collection and processing of clinical samples.
CO2 To learn the techniques for isolation and identification of bacterial pathogens.
CO3 To gain expertise in various techniques of clinically important viral pathogens and their
identification.
CO4 To get acquainted with medically important fungi and their metabolism.
CO5 To categorize parasites and understand their role in infections.

Hours Objectives
I 1. Collection and Transport of Clinical specimens. 12
2. Simple, Differential and Special staining of Clinical materials. CO1
3. Culture techniques used to isolate microorganisms.
II 4. Identification of bacterial pathogens by their biochemical reactions. 12 CO2
5. Antimicrobial susceptibility testing by disc-diffusion technique and
determination of Minimum Inhibitory Concentration.
III 6. Isolation of Bacteriophages from Sewage and other natural sources. 12 CO3
7. Identification of Viruses in Slides/Smears/Spotters. Demonstration
of Negri bodies (Staining).
8. Cultivation of Viruses in Embryonated eggs – Amniotic, Allantoic,
Yolk sac routes and Chorio-allantoic membrane.
IV 9. Microscopic identification of medically important Fungi – KOH 12 CO4
and Lactophenol cotton Blue staining.
10. Slide culture techniques for fungal Identification
11. Identification of Dermatophytes.
12. Germ tube test, Carbohydrate fermentation and assimilation tests
for Yeasts.
V 13. Direct Examination of Faeces – wet mount and Iodine mount – 12 CO5
Demonstration of Protozoan cysts and Helminthes eggs.
83
14. Concentration techniques of stool specimen – Floatation and

Sedimentation methods.
15. Examination of blood for Malarial parasites – thin and thick smear
preparations.
16. Identification of Medically important parasites in slides / specimens
as spotters.
Total 60
Course Outcomes

Outcomes
CO1 Demonstrate methods to observe and measure microorganisms by PO4, PO5, PO7.
standard microbiological techniques
CO2 Identify pathogenic microorganisms in the laboratory set-up and PO4, PO5, PO7, PO8.
interpret their sensitivity towards commonly administered
antibiotics.
CO3 Understand experimental tools used to cultivate and characterize PO4, PO5, PO7, PO8.
clinically important viruses and bacteriophages
CO4 Elucidate clinically important fungi. PO4, PO5, PO7, PO8.
CO5 Investigate Parasites of medical importance and identify them PO4, PO5, PO7, PO8.
from clinical specimens.
Text Books
1. Dubey, R.C. and Maheswari, D.K. (2020). S. Chand Publishers. ISBN-13: 978-8121921534,
ISBN-10: 8121921538.
2. K.R. Aneja (2017). Experiments in Microbiology, Plant Pathology, Tissue Culture and
Microbial Biotechnology. 5th Edition. New Age International Publishers. ISBN-10:
9386418304, ISBN-13: 978-9386418302.
3 Collee, J.G., Fraser, A.G., Marnion, B.P. and Simmons, A. (1996). Mackie & McCartney
Practical Medical Microbiology. 14th Edition. Elsevier. ISBN-10: 813120393X, ISBN-13: 978-
8131203934.
4 Prince CP (2009). Practical Manual of Medical Microbiology, Ist edition, Jaypee digital
publishing.
5 James H. Jorgensen, Karen C. Carroll, Guido Funke, Michael A. Pfaller, Marie Louise Landry,
Sandra S. Richter, David W. Warnock (2015). Manual of Clinical Microbiology, 11th Edition,
ASM press
References Books
84
1 Patricia M. Tille (2021). Bailey & Scott’s Diagnostic Microbiology, 15th Edition. Elsevier.
ISBN-10: 0323681050, ISBN-13: 978-0323681056.
2 Monica Cheesbrough (2006). District Laboratory Practice in Tropical Countries. Part 1. 2nd
Edition. Cambridge University Press. ISBN-10: 0521171571, ISBN-13: 978-0521171571.
3 Michael A. Pfaller (ed.) (2015). Manual of Clinical Microbiology. Vol. 1 and 2. 11th Edition.
ASM Press. ISBN-10: 9781555817374, ISBN-13: 978-1555817374.
4 Josephine A. Morello, Paul A. Granato and Helen EckelMizer (2002). Laboratory Manual and
Workbook in Microbiology. 7th Edition. The McGraw Hill Company. ISBN: 0-07-246354-6.
5 Rowland, S.S., Walsh, S.R., Teel, L.D. and Carnahan, A.M. ((1994). Pathogenic and Clinical
Microbiology: A Laboratory Manual. Lippincott Williams & Wilkins. ISBN-10: 0316760498,
ISBN-13: 9780316760492.
Web Resources
1 https://www.microcarelab.in/media/microcarelab.in/files/Sample-Collection-Manual.pdf
2 http://ssu.ac.ir/cms/fileadmin/user_upload/Daneshkadaha/pezeshki/microb/file_amuzeshi/
Lab_QA_Microbiology_QA.pdf
3 https://www.academia.edu/11977315/Basic_Laboratory_Procedures_in_Clinical_Bacteriology
4 https://cmr.asm.org/content/31/3/e00062-17.full.pdf
5 https://microbiologyinfo.com/techniques-of-virus-cultivation/

Internal
Evaluation Assignments
Seminars

External
Evaluation
Total 100 Marks

Understand/ MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend (K2) overview
Suggest idea/concept with examples, Suggest formulae, Solve problems,
Application (K3)
Observe, Explain
85

Analyze (K4)
Create (K6)
Presentations
CO1 S M S
CO2 S S S L
CO3 S S S L
CO4 S S S L
CO5 S S S L

Code s Hour CI Exter Total
s A nal
GROUP Project - - - - 4 5 40 60 100
PROJECT with
Viva-
Voce
CC-XII
Group projects enable students to get hands-on training in microbiological techniques needed for
research. Thus the students can share diverse perspectives resulting in pooling of knowledge and
skills. Group work may approach tasks and solve problems in novel, interesting ways, thereby
converting established theoretical concepts to practical skills. If structured properly, it will
promote team work and collaboration. Group projects also will help students to choose a
research design, solve real life problems and benefit the society at large. Thus group project
facilitates the students to convert ideas to practice thereby creating a research culture among
students.
86
Guidelines for group project:
A research problem need to be selected based on creative ability and scientific thought.
A brief description of the problem needs to be given.
Hypothesis statement should be framed.
Objectives by which the project work is to be carried out should be clearly stated.
Methodology has to be designed to test the hypothesis.
Results obtained need to be replicable.
Documented report has to be submitted on completion of the project.

s A nal
RECOMBINANT ELECTI Y - - 3 4 25 75 100
DNA VE
TECHNOLOGY GENERI
C/
DISCIP
LINE
SPECIFI
C
ELECTI
VE- V
Course Objectives
CO1 Understand the principles of rDNA technology.

CO2 Illustrate the molecular tools employed in gene cloning.
CO3 Discuss the importance of various molecular techniques and their importance in
Biotechnology.
CO4 Acquire knowledge about the concepts of tissue culture methods and transgenic
organisms.
CO5 Examine recent trends in genetic engineering and its application in human welfare.
Hours Objectives
I MilestonesinrDNATechnology-GeneManipulation- 12
StepsinvolvedinGeneCloning.Isolation of Chromosomal and CO1
87
Plasmid DNA. Restriction endonuclease - Discovery,

Types,Mode of action-Application of
Ligase,DNAPolymerase,DNA Modifying
enzymesandTopoisomerases.UseofLinkersandAdapters.
II ArtificialGeneTransfermethods- 12 CO2
CalciumChlorideInduction,Electroporation,Microinjection,
Biolistic method, Liposome and Viral-mediated
delivery.Cloning vectors –Properties and Applications -
Plasmid Based Vectors- Natural Vectors-pSC101 and
pMB1.Artificial Vectors- pBR322 and pUC. Phage Based
Vectors- Lambda phage. Hybrid Vectors,Phagemid, Cosmid,
BAC and YAC.Screening of Recombinants.Genomic DNA
and cDNAlibrary-ConstructionandScreening.
III Molecular Tools- PCR- Types. Gel Electrophoresis- AGE 12 CO3
and PAGEBlottingTechniques-
Southern,Western&Northern.DNAsequencingmethods-
Sanger’sandAutomated method. Recent Trends in Genetic
Engineering- Targeted Genome Editing- ZFNs,TALENs,
CRISPRs. Gene Targeting-Knock-in &Knock-outs.DNA
Finger Printing,
IV Plant Biotechnology – Media, Growth Regulators and 12 CO4
Equipment for Plant Tissue Culture-Explant Culture-
Micropropagation- Callus and Protoplast Culture-
Production of Bio-Active Secondary Metabolites by Plant
Tissue Culture -Agrobacterium and Crown Gall Tumors,
TiPlasmidandRiPlasmid-AnimalBiotechnology-
PrinciplesofAnimalCellCulture,MediaandEquipment for
Animal Cell Culture – Primary and Secondary Cultures-
Cell Lines- Types, Establishment and Maintenance of Cell
Lines.
V Applications of Genetic Engineering - Transgenic Animals 12 CO5
– Mice and Sheep-RecombinantCytokines and their use in
the Treatment of Animal infections- Monoclonal Antibodies
inTherapy- Vaccines and their Applications in Animal
Infections - Human Gene Therapy-
GermlineandSomaticCellTherapy-Ex-vivoGeneTherapy-
SCID(SevereCombinedImmunoDeficiency) – In-vivo Gene
Therapy- CFTR (Cystic Fibrosis Transmembrane
Regulator) –Vectors in Gene Therapy-Viral and Non-Viral
Vectors. Transgenic Plants– BtCotton, BtCorn, Round
88
Ready soy bean, Flavr Savr Tomato and Golden Rice.

Total 60
Course Outcomes
Outcomes
CO1 Illustrate the steps involved in introduction and expression PO4, PO6, PO7, PO9
of foreign DNA into bacteria, animal and plants cells and
their screening.
CO2 Discuss the various cloning vectors and their applications. PO4, PO6, PO7, PO9
CO3 Assess the usage and advantages of molecular tools. PO4, PO6, PO7, PO9
CO4 Explain plant and animal tissue culture protocols and gene PO4, PO6, PO7, PO9
transfer mechanism.
CO5 Elucidate and understand the application of genetic PO4, PO6, PO7, PO9
engineering and gene therapy.
Text Books
1. Brown T.A.(2016). Gene Cloning and DNA Analysis. 7thEdition . John Wiley and Jones,
Ltd.
2. Dale J. W., Schantz M.V. and Plant N. (2012). From Gene to Genomes – Concepts and
Applications of DNA Technology. 3rd Edition. John Wileys and Sons Ltd.
3. KeyaChaudhuri (2013). Recombinant DNA technology. The Energy and Resources Institute
4. SiddraIjaz, Imran UlHaq (2019). Recombinant DNA Technology. Cambridge Scholars
Publishing.
5. Monika Jain (2012). Recombinant DNA Techniques: A Textbook, I Edition,Alpha Science
International Ltd
References Books
1. Maloy S. R., Cronan J.E. Jr. and FreifelderD.(2011). Microbial Genetics. 2nd Edition.
Narosa Publishing Home Pvt Ltd.
2. Glick B. R. and Patten C.L.(2018). Molecular Biotechnology – Principles and Applications
of Recombinant DNA. 5th Edition. ASM Press.
3. Russell P.J. (2010). iGenetics - A Molecular Approach, 3 rd Edition. Pearson New
International Edition.
4. Synder L., Peters J. E., Henkin T.M. and Champness W. (2013). Molecular Genetics of
Bacteria,4th Edition. ASM Press Washington-D.C. ASM Press.
5. James D.Watson, Michael Gilman, Jan Witkowski, Mark Zoller (1992). Recombinant
DNA. Scientific American Books
Web Resources
1 https://www.britannica.com/recombinant-DNA-technology
2 https://www.byjus.com/recombinant-dna-technology
3 https://www..rpi.edu
89
4 https://www..ncbi.nlm.nih.gov
5 https://www.le.ac.uk/recombinant-dna-and-genetic-techniques
Evaluation Seminars
External
Evaluation
Total 100 Marks

Understand /
Comprehend
overview
(K2)
Presentations

CO1 S L S S M S
CO2 S L S S M S
CO3 S L S S M S
CO4 S L S S M S
CO5 S L S S M S
90
Subject Subject Category L T P S Cr Inst. Marks

Code Name edi Hour CI Exter Total
ts s A nal
BIOSAFETY CORE Y - - - 3 4 25 75 100
&BIOETHIC ELECTIV
S E VI
Course Objectives
CO1 To create a research environment - encourage investigation, analysis and studying the bioethical
principles, values, concepts, and social and juridical implications contained in the Universal
Declaration on Bioethics and Human
CO2 Rights in order to assist their application and promotion in the areas of science, biotechnology
and medicine.
CO3 To discuss about various aspects of biosafety regulations, IPR and bioethics concerns arising
from the commercialization of biotech products.
CO4 To introduce fundamental aspects of Intellectual property Rights to students who are going to
play a major role in development and management of innovative projects in industries.
CO5 To understand the importance of IPR, Patents and Patent laws.
Hours Objectives
I Basics of Biosafety - Laboratory Hazards and Hazard symbols. 12 CO1
Definitions on Biohazard, Biosafety and Biosecurity- Biohazard- LAI,
BP. Biohazard Classification. Biological Risk Groups. Need and
application of biosafety. Good Laboratory Practices (GLP),
Good Manufacturing Practices (GMP).
II Hazardous materials in Biotechnology - Categories of Waste in the 12 CO2
Biotechnology Laboratories, Biohazardous waste and their disposal and
treatments- issues in use of GMO’s, risk for animal/human/ agriculture
and environment owing to GMO. Hazardous materials, Emergency
response/ first aids in Laboratories.
III Biological Safety Containment in Laboratory - Primary and secondary 12 CO3
containments - Physical and biological containment. Types of biosafety
containments (level I, II, III), PPE, Biosafety guidelines in India - Roles
of Institutional Biosafety Committee, RCGM, GEAC.
IV Introduction and need of Bioethics - its relationship with other branches, 12 CO4
Ethical implications of biotechnological products and techniques. Ethical
Issues involving human cloning, human genome project, prenatal
diagnosis, agriculture and animal rights, Social and ethical implications
of biological weapons.
91
V IPR, Patents and Patent laws - Intellectual property rights-TRIP- GATT 12 CO5
International conventions patents, Methods of application of patents,
Legal implications. Biodiversity and farmer rights, Objectives of the
patent system, Basic principles and general requirements of patent law,
Biotechnological inventions, and patent law. Legal development-
Patentable subjects and protection in biotechnology. The patenting of
living organisms.
Total 60
Course Outcomes
Outcomes
CO1 Understand the control measures of laboratory hazards (chemical, PO1, PO2, PO3, PO7,
biological and physical) and to practice safety strategies and PO10
personal protective equipment
CO2 Develop stratagems for the use of genetically modified organisms PO1, PO3, PO4
and Hazardous materials
CO3 Develop skills of critical ethical analysis of contemporary moral PO1, PO6
problems in medicine and health care.
CO4 Analyze and respond to the comments of other students regarding PO3, PO4
philosophical issues.
CO5 Pave the way for the students to catch up Intellectual Property(IP) as PO1, PO7, PO10
a career option a. R&D IP Counsel b. Government Jobs – Patent
Examiner c. Private Jobs d. Patent agent and Trademark agent e.
Entrepreneur
Text Books
1. Usharani .B, S Anbazhagi, C K Vidya, (2019). Biosafety in Microbiological Laboratories- 1 st
Edition, Notion Press, ISBN-101645878856
2. Satheesh.M.K.,(2009). Bioethics and Biosafety- 1st Edition, J. K International Publishing House
Pvt. Ltd: Delhi, ISBN :9788190675703
3 DeepaGoel and ShominiParashar, (2013). IPR, Biosaftey and Bioethics- 1 st Edition, Pearson
education: Chennai, ISBN-13: 978-8131774700
4 Rajmohan Joshi (2006). Biosafety and Bioethics. Gyan Books publisher.
5 Sateesh. M.K. (2013). Bioethics and Biosafety. i.K. International pvt,Ltd.
References Books
1 Nithyananda, K V. (2019). Intellectual Property Rights: Protection and Management, India, IN:
Cengage Learning India Private Limited, ISBN-10: 9386668572
2 Neeraj, P., &Khusdeep, D. (2014). Intellectual Property Rights, India, IN: PHI learning Private
Limited, ISBN : 9788120349896
3 Ahuja, V K. (2017). Law relating to Intellectual Property Rights, India, IN: Lexis Nexis, ISBN-10:
8131251659.
4 Edited by Sylvia Uzochukwu, Nwadiuto (Diuto) Esiobu, Arinze Stanley Okoli, Emeka Godfrey
92
Nwoba, EzebuiroNwagboChristpeace, Charles OluwaseunAdetunji, Abdulrazak B. Ibrahim,

Benjamin EwaUbi (2022). Biosafety and Bioethics in Biotechnology-Policy, Advocacy, and
Capacity Building,1st edition. CRC Press
5 Sree Krishna. V (2007). Bioethics and Biosafety in Biotechnology. New age international
publishers.
Web Resources
1 Subramanian, N., &Sundararaman, M. (2018). Intellectual Property Rights – An Overview.
Retrieved from http://www.bdu.ac.in/cells/ipr/docs/ipr-eng-ebook.pdf.
2 World Intellectual Property Organisation. (2004). WIPO Intellectual propertyHandbook. Retrieved
from https://www.wipo.int/edocs/pubdocs/en/intproperty/489/wipo_pub _489.pdf.
3 https://www..niehs.nih.gov/bioethics
4 https://www.sist.sathyabama.ac.in
5 https://www.longdom.org/bioethics-and-biosafety
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand/ MCQ, True/False, Short essays, Concept explanations, Short summary or
Comprehend (K2) overview
Application (K3)
Observe, Explain
Analyze (K4)
Create (K6)
Presentations
PO1 PO2 PO3 PO PO5 PO6 PO7 PO PO9 PO10

4 8
CO1 S S S M M
CO2 S S S
CO3 S S
93
CO4 S S
CO5 S M S
VI - SEMESTER
Subject Subject Name Cate L T P S Credit Inst. Marks
Code gory s Hour CI Exter Total
s A nal
ENVIRONMENTAL COR Y - - - 4 6 25 75 100
AND E
AGRICULTURE COU
MICROBIOLOGY RSE
–XIII
Course Objectives
CO1 To discuss the distribution and association of microorganism in various ecosystems and to know
about the role of microorganism in water pollution and water quality.
CO2 To acquire knowledge about the role of microorganism in water pollution and water quality
CO3 Gain knowledge about microbes as biofertilizers and the aspects of application.
CO4 To learn about the process of solid waste management and sewage water treatment.
CO5 Gain knowledge on various plant diseases and pathogens
Hours Objectives
I Microorganisms and their Habitats: Structure and function of ecosystems 12 CO1
Terrestrial Environment: Soil profile and soil microflora, Microbial
succession in decomposition of soil organic matter. Role of
microorganisms in elemental cycles in nature: Carbon, Nitrogen.
Aquatic Environment: Microflora of fresh water and marine habitats,
factors influencing microbial growth in the aquatic environments.
Atmosphere: Aeromicroflora and dispersal of microbes, Assessment of
air quality, Enumeration of microorganism in air, Air sanitation.
Extreme Habitats: Extremophiles: Microbes thriving at high & low
temperatures, pH, high hydrostatic & osmotic pressures, salinity, & low
nutrient levels.
Predisposing factors for Environmental diseases – infectious (water and
air borne) and pollution related, spread and control of these diseases.
Environmental Protection Agency (EPA) - role in environmental
protection.
II Water potability: Sources and types of water surface, ground, stored, 11 CO2
distilled, mineral and de-mineralized water and their pollution, biological
indicators of water Pollution, Eutrophication. Conventional
94
Bacteriological standards of Water Quality, MPN index, coliform test,

Membrane filtration. BOD, COD. Advanced molecular methods for
water analysis. Water borne diseases. Central Pollution Control Board
(CPCB) standards.
III Microbial Interactions: Rhizospheremicroflora. Concepts of Nitrogen 12 CO3
fixation – Symbiotic and asymbiotic nitrogen fixers.Brief account of
microbial interactions: Symbiosis, neutralism, commensalism,
competition, Ammensalism, Synergism, parasitism, and predation.
General account and Significance of Biofertilizers and biocontrol agents
– Bacterial, cyanobacterial, VAM. Mass production of Rhizobial
biofertilizer. Biocontrol agents – Bacterial, viral, fungal.
IV Waste treatment and bioremediation: Solid waste management: Sources 15 CO4
and types of solid waste, composting, vermin composting, production of
biogas. Liquid waste management: Primary, secondary, and tertiary
sewage treatment. Bioremediation and waste management: Need and
scope of bioremediation. Degradation of hydrocarbons, oil spills, heavy
metals – Chromium, lead, and xenobiotics – PCB.
V Plant pathology: Mode of entry of pathogens, Microbial enzymes, toxins, 10 CO5
growth regulators and suppressor of plant defense in plant diseases. Plant
defense mechanisms. Bacterial diseases – Citrus canker, Blight of paddy.
Viral disease – TMV, CMV. Fungal disease- red rot of sugarcane, Tikka
disease. Plant disease management.
Total 60
Course Outcomes

Outcomes
CO1 Describe about the structure and function of ecosystems and PO1
understand the role of microbes in various environments
CO2 Identify the cause of water pollution, and perform methods to assess PO4,PO5,PO6,PO7,
the quality of water. PO8
CO3 Explain the productionofbiofertilizers and biopesticides. PO1, PO7,PO8
CO4 Explainabout waste treatment process and microbial decomposition PO6
and bio-remediation process.
CO5 Describe about plant diseases caused by microbes and acquire a clear PO1,PO5
idea on plant pathogenic interaction
Text Books
1. Joseph C. Daniel. (2006). Environmental aspects of Microbiology 2nd Edition. BrightSun
Publications.
95
2. Pradipta. K.M. (2008). Textbook of Environmental Microbiology.I.K.Publishing. House.

3. Ramanathan, and Muthukaruppan SM. (2005). Environmental Microbiology.Om
SakthiPathipagam, Annamalai Nagar.
4. K. Vijaya Ramesh.(2004).Environmental Microbiology. 1st Edition. MJP Publishers.
5. SubbaRao.N.S.(2017). Soil Microbiology.4th Edition. Oxford and IBH Publishing Pvt.Ltd.

References Books
1 Dirk, J. Elasas, V., Trevors, J.T., Wellington, E.M.H. (1997). Modern Soil
Microbiology, Marcel Dekker INC, New York, Hong Kong.
2 EcEldowney S, Hardman D.J., Waite D.J., Waite S.(1993). Pollution: Ecology and
Biotreatment – Longman Scientific Technical.
3 Mitchel, R.(1992). Environmental Microbiology. Wiley –John Wiley and Sons. Inc.
Publications, New York.
4 Clescri, L.S., Greenberg, A.E. and Eaton, A.D.(1998). Standard Methods for
Examination of Water and Wastewater, 20thEdition. American Public Health Association.
5 Atlas, R.M. and Bartha, R.(1992). Microbial Ecology: Fundamentals andApplications, 2nd Edition.
The Benjamin / Cummings Publishing Co.,Redwood City, CA.
Web Resources
2 https://www.classcentral.com/course/swayam-plant-pathology-and-soil-health-14236
3 https://www.wasteonline.org.uk/resources/InformationSheets/WasteDisposal.htm
4 https://plantpath.cornell.edu/labs/enelson/PDFs/Hill_et_al_2000.pdf
5 https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2389.2005.00781.x
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
(K2)
(K3) Explain
Analyse (K4) Problem-solving questions, Finish a procedure in many steps, Differentiate between
96

Presentations

CO1 S
CO2 M S S S S
CO3 S S S
CO4 S
CO5 M M
97
Subject Subject Name Catego L T P S Cr Inst. Marks

Code ry edi Hour CI Exter Total
ts s A nal
FOOD, DAIRY CORE Y - - - 4 6 25 75 100
AND PROBIOTIC COUR
MICROBIOLOGY SE –
XIV
Course Objectives
CO1 To impart current knowledge of basic and applied microbiological aspects of fluid milks and
dairy products for improved quality and food safety.
CO2 Gives an insight into various types of food borne diseases and their prevention
CO3 To gain information about microflora of milk
CO4 To study about the production of fermented dairy products
CO5 To impart current knowledge of probiotics, prebiotics and functional dairy foods for the health
benefits
To create a sustainable environmentally and technologically advanced dairy farm
Hours Objectives
I Food as a substrate for micro organisms-.Micro organisms important 12
in food microbiology; Molds, yeasts and bacteria -General CO1
Characteristics - Classification and importance. Principles of food
preservation - Asepsis - Removal of micro organisms, - High
temperature - Low temperature - Drying - Food additives.
Nanoscience in food preservation; microencapsulation.
II Contamination and spoilage of food products -Food borne infections 15 CO2
(Bacillus cereus, ,Salmonellosis, Shigellosis, ,Listeria monocytogenes
and Campylobacter jejuni) and intoxications – (Staphylococcus
aureus, Clostridium botulinum ,Clostridium perfringens and
mycotoxins) Food borne disease outbreaks - newly emerging
pathogens. Conventional and Novel technology in control of food
borne pathogens and preventive measures - Food sanitation - plant
sanitation - Employees’ health standards. Regulatory Agencies
&criteria for food safety.
III Microflora of raw milk - sources of contamination. Spoilage and 15 CO3
preservation of milk and milk products. -antimicrobial systems in raw
milk. Importance of biofilms, their role in transmission of pathogens
in dairy products and preventive strategies.
IV Food fermentations: Indian Pickles Bread,vinegar, fermented 15 CO4
98
vegetables (sauerkraut), fermented dairy products (yoghurt, cheese,

AcidophilusMilk,Kefir,Koumiss). Oriental fermented foods-Miso –
Tempeh Ontjom . Natto, Idli Spoilage and defects of fermented dairy
products -. Functional fermented foods and nutraceuticals, bioactive
proteins and bioactive peptides, genetically modified foods.
V Probiotic microorganisms, concept, definition safety of probiotic 15 CO5
microorganisms, legal status of probiotics Characteristics of Probiotics
for selection: stability maintenance of probiotic microorganisms. Role
of probiotics in health and disease: Mechanism of probiotics.
Application of bacteriocins in foods.Biopreservation. Prebiotics:
concept, definition, criteria, types and sources of prebiotics, prebiotics
and gut microflora - Prebiotics and health benefits: mineral
absorption, immune response, cancer prevention, elderly health and
infant health, prebiotics in foods.
Total 72
Course Outcomes
Cour On completion of this course, students will;
se
Outc
omes
CO1 Gain knowledge about food as a substrate for various microbes, PO7,PO8,PO10
Understand about the principles and application of different types of
food spoilage and preservation technique,
CO2 Acquire a thorough understanding of food borne diseases, testing PO5,PO10
methods, and preventive technique
CO3 Gain information about spoilage of milk and its products and its PO5,PO7
antimicrobial properties
CO4 Learn about the various fermented product and its various stage PO7,PO8,PO10
spoilage
CO5 Impart current knowledge of probiotics, prebiotics and functional dairy PO5,PO6
foods for the health benefits
Text Books
1. Frazier WC and West off DC. (2017). Food microbiology. 5th Edition TATA McGraw Hill
Publishing Company Ltd. New Delhi.
2. Adams, M.R., Moss, M.O.(2018). Food Microbiology 1 stedition. New Age Publishers by New
Age International (P) Ltd., Publishers.
3 R.C. Dubey. (2014). Advanced Biotechnology. S. Chand publishers.
4 Banwart GJ. (1989). Basic food microbiology, Chapman & Hall, New York.
5 Sugumar D. (1997). Outlines of dairy technology, Oxford University press. 1997.
References Books
99
1 Jay JM, Loessner MJ and Golden DA.(2005). Modern Food Microbiology. 7th Edition
CBS Publishers and Distributors, Delhi, India.
2 Prescott, Harley and Klein Wim.(2008). Microbiology, 7th Edition McGraw Hill Publications.
3 Robinson, R. K.(2002). Dairy Microbiology Handbook - The Microbiology of Milk and Milk
Products (Third Edition), A John Wiley & Sons, Inc., New York.
4 Yuankunlee,Sepposalminen. (2008). Handbook of probiotics and prebiotics Second Edition. A
John Wiley & Sons publication Inc.
5 DharumauraiDhansekaran, AlwarappanSankaranarayanan. (2021). Advances in Probiotics
Microorganisms in Food and Health 1st Edition. eBook ISBN:9780128230916.
WEB RESOURCES
1 https://www.researchgate.net/publication/
15326559_A_Dynamic_Approach_to_Predicting_BacterialGrowth_in_Food/link/
5a1d2e02aca2726120 b28eba/download
2 https://www.fda.gov/food/laboratory-methods-food/bam-foodsamplingpreparation-sample-
homogenate
3 https://www.researchgate.net/publication/243462186_Foodborne_diseases_in_India_-
_A_review
4 https://www.researchgate.net/publication/
228662659_Fermented_Dairy_Products_Starter_Cultures_and_Potential_Nutritional_Benefits/
link/000084160cf23f86393d5764/
download
5 https://www.fda.gov/food
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
(K2)
100
(K3) Explain
Analyse Problem-solving questions, Finish a procedure in many steps, Differentiate between
Evaluate
(K5)
Presentations
CO1 S S M
CO2 S M
CO3 S M
CO4 S S M
CO5 M M
101

s A nal
PRACTICAL CORE Y - - - 4 6 25 75 100
VI COURSE
– XV-
PRACTI
CAL VI
Course Objectives
CO1 Toassess the water quality and potability.
CO2 To acquire knowledge on enumeration of bacteria from milk and milk quality analysis
CO3 To investigate various extracellular enzyme producers in soil and to gain knowledge on
preparation of biofertilizers
CO4 Improve knowledge on plant pathogens
CO5 To acquire knowledge on preparation of probiotics and prebiotics
Hours Objectives
I 1. Physical, chemical, and microbiological assessment of water and 12
potability test for water. CO1
o Physical – Color, pH,
o Chemical - alkalinity, acidity, DO, BOD, COD
o Microbiological – MPN index (Presumptive, Completed and
Confirmatory test)
2. Study of air microflora by settle plate method.
II 3. Isolation and identification of bacteria and fungi from fruits and 12 CO2
vegetables
4. Direct microscopic count of milk.
5. Methylene blue reductase test and Resazurin test
6. Microbiological examination of milk by SPC.
III 7. Isolation of extracellular enzyme producers –Amylase, protease, 12 CO3
lipase
8. Microbiological assay of antibiotics by cup plate method and other
methods
9. Isolation of Rhizobium/ Azotobacter/ phosphate solubilizing
organisms
10. Preparation of biofertilizers – Demonstration
IV 11. Study of plant pathogens- Tikka Disease, Red rot of sugarcane, 10 CO4
102
Citrus canker, Blight of paddy.

12. Study of fungi - Mucor,Curvularia, Alternaria, Rhizopus,
Aspergillus
V 13. Isolation of constituent flora of fermented milk. 14
14. Growth of probiotic LAB in broth, milk and whey. CO5
15. Preparation of probiotic fermented milks like dahi, yoghurt, lassi
and whey drink.
16. Effect of prebiotics on the growth of LAB in milk and broth.
17. Survivability of probiotic organisms in fermented milks.
18. Antimicrobial potential of the functional dairy products.
Total 60
Course Outcomes
Outcomes
CO1 Assess the microbial quality of water and relate the experimental PO1,
results to the prescribed standards by the statutory bodies PO4,PO5,PO6,
PO7, PO8
CO2 Evaluate the quality of milk and enumerate bacteria in milk by PO5,PO6, PO7,
standard plate count method PO8
CO3 Identify extracellular enzyme producing and nitrogen fixing PO1,PO8
microorganism form soil and to prepare a biofertilizer.
CO4 Identifyvarious plant pathogenic bacteria PO1
CO5 Synthesize probiotic fermented milks using microorganisms PO1,PO7,PO8
Text Books
1. Cappucino J and Sherman N.(2010). Microbiology: A Laboratory Manual. 9th Edition. Pearson
Education Limited.
2. Kannan. N. (1996). Laboratory manual in General Microbiology. Palani Publications.
3. R C Dubey and D K Maheswari.(2002). Practical Microbiology. S. Chand Publishing.
4. NeelimaGarg, K.L. Garg, K.G. Mukerji (2010).Laboratory Manual of Food Microbiology,
Wiley publication
5. Aneja, KR.(2010). Experiments in Microbiology, Plant pathology and Biotechnology.
New Age International (P) Limited.
References Books
1 Christon J. Hurst Editor in Chief, Ronald L. Crawford, Jay L. Garland, David A. Lipson, Aaron
L. Mills, Linda D. Stetzenbach (2007). Manual of Environmental Microbiology, Third
Edition,Wiley publication.
2 James G Cappucino and Natalie Sherman.(2016). Microbiology – A laboratory
manual. 4thEdition. The Benjamin publishing company, New York.
3 Marylynn V. Yates, Cindy H. Nakatsu, Robert V. Miller, Suresh D. Pillai 2016). Manual of
103
Environmental Microbiology, 4thEdition,ASM press.

4 Burns, Richard G (2005). Environmental MicrobiologyA Laboratory Manual, 2 nd
Edition .Lippincott Williams & Wilkins, Inc.
5 Ian Pepper, Charles Gerba, Jeffrey Brendecke (2004). Environmental Microbiology-A
laboratory manual, Elsevier.
Web Resources
1 https://micobenotes.com/fields-of-microbiology/
2 https://bio.libretexts.org
3 https://www.google.com
4 https://www.sfamjournals.onlinelibrary.wiley.com
5 https://www.degruyter.com
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
(K2)
(K3) Explain
Presentations
PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8
CO1 S M S S S S
CO2 M M M M
CO3 M S
104
CO4 M
CO5 M S S
ELECTIVE GENERIC /DISCIPLINE SPECIFIC ELECTIVE- VIII-

PHARMACEUTICAL MICROBIOLOGY
Code s Hour CI Ext Total
s A ern
al
PHARMACE ELECTIVE Y - - - 2 4 25 75 100
UTICAL GENERIC
MICROBIOL /DISCIPLINE
OGY SPECIFIC
ELECTIVE- VII-
Course Objectives
CO1 To provide the knowledge on basics of chemotherapy
CO2 To learn the assays and testing methods of antibiotics.
CO3 To gain information about spoilage of pharmaceutical products
CO4 To provide the knowledge on drug discovery and clinical trials
CO5 To learn about regulations in pharmaceutical industry
Hours Objectives
I Introduction to Pharmaceutical microbiology: Ecology of 12 CO1
microorganisms in pharmaceutical industry: Atmosphere, water, skin
and respiratory flora of workers, raw materials, packaging, building and
equipments and their control measures; Design and layout of sterile
manufacturing.
II Microbial contamination and spoilage of pharmaceutical products: 10 CO2
Microbial aspects of pharmaceutical products; Sterilization of
pharmaceutical products: Heat, gaseous, radiation and filtration;
Contamination and Spoilage of Pharmaceutical products: sterile
injectable and non-injectable, ophthalmologic preparation, implants.
III Production of antibiotics: Production of antibacterial – Penicillin, 12 CO3
Tetracycline; antifungal – Griseofulvin, Amphotericin; antiparasitic
agents – Artemesin, Metronidazole; Semi-synthetic antibiotics and
anticancerous agents; Additional application of microorganisms in
pharmaceutical sciences: Enzymes- Streptokinase, Streptodornase, L-
asperginase and clinical dextrin; Immobilization procedures for
pharmaceutical applications (liposomes); Biosensors in pharmaceuticals.
105
IV Production of immunological products and their quality control: 16 CO4

Vaccines - DNA vaccines, synthetic peptide vaccines, multivalent
vaccines; Vaccine clinical trials; Immunodiagnostics - immuno sera and
immunoglobulin; Quality control in Pharmaceutical: In – Process and
Final Product Control; Sterility tests.
V Quality Assurance and Validation:Good Manufacturing Practices 10 CO5
(GMP) and Good Laboratory Practices (GLP) in pharmaceutical
industry; Regulatory aspects of quality control; Quality assurance and
quality management in pharmaceuticals – BIS (IS), ISI, ISO, WHO and
US certification.
Total 60
Course Outcomes
Outcomes
CO1 Learn the basics of chemotherapy and action of antibiotics PO1,PO10
CO2 Carry out the microbiological assay of antibiotics PO7
CO3 Analyse Microbiological standardization of Pharmaceuticals PO5,PO8,PO10
,sterility testing of pharmaceutical
productsApplysterilization in pharmaceutical industry
CO4 Evaluate the process and develop new strategies for rational PO9,PO10
drug design
CO5 Learn the Regulatory guidelines in pharmaceuticals product. PO3,PO5
Text Books
1. Chand Pasha Kedernath. (2021). Text book of Pharmaceutical Microbiology. Ramnath Publisher.
2. Hugo WB and Russell AD. (2004).Pharmaceutical Microbiology VII edition. Blackwell Scientific
Publication, Oxford.
3 Franklin,DJ. and Snow, GA. (2013). Biochemistry of antimicrobial action.Chapman& Hall.
4 Kuntal Das (2019). Pharmaceutical Microbiology, second edition, NiraliPrakashan.
5 PriyatamaPowar, ShitalNimbargi, VaijayantiSapre (2020). Pharmaceutical Microbiology, I
edition, Technical publications.
References Books
1 Handa, S.S. and Kapoor, V.K. (2022) .Pharamcognosy. 4thEdition.VallabhPrakashanPublishers,
New Delhi.
2 Kokate, C.K., Durohit, A.P. and Gokhale, S.R.,(2002). Pharmacognosy. 12thedition
NiraliPrakasham Publishers, Pune.
3 S. P. Vyas& V. K. Dixit.(2003). Pharmaceutical Biotechnology. CBS Publishers & Distributors,
New Delhi.
4 Wallis, T.E. (2005). Text book of Pharmacognosy. 5th edition. CBS publishers and distributors,
New Delhi.
106
5 Garrod, L.P., Lambert, HP. And C’Grady, F. (1973). Antibiotics and Chemotherapy. (eds).
Churchill Livingstone.
Web Resources
1 https://www.pharmapproach.com/introduction-to-pharmaceutical-microbiology/
2 https://www.iptsalipur.org/wp-content/uploads/2020/08/BP303T_PMB_UNIT_I.pdf
3 https://www.pharmanotes.org/2021/11/pharmaceutical-microbiology-b-pharma.html
4 https://snscourseware.org/snscphs/notes.php?cw=CW_604b15c6313c5
5 https://www.thermofisher.com
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
Understand /
(K2)
(K3) Explain
Presentations

CO1 M M
CO2 M
CO3 S M M
CO L M
4
CO5 L M
107

s A nal
ENTREPREN ELECTIVE Y - - - 2 4 25 75 100
EURSHIP GENERIC
AND BIO- /DISCIPLIN
BUSINESS E SPECIFIC
ELECTIVE-
VIII
Course Objectives
CO1 Understanding basic concepts in the area of entrepreneurship, the role and importance of
entrepreneurship for economic development
CO2 Developing personal creativity and entrepreneurial initiative, adopting the key steps in the
elaboration of business idea.
CO3 Understanding the stages of the entrepreneurial process and the resources needed for the
successful development of entrepreneurial ventures.
CO4 Explain the central components of successful business strategies in biotechnology, and
create a business plan.
CO5 Understand the various funding resources and develop as Entrepreneur
Hours Objectives
I Bio Entrepreneurship: Introduction to bio-business, SWOT 12 CO1
analysis of bio-business. Ownership, Development of
Entrepreneurship; Stages in entrepreneurial process; Government
schemes and funding. Small scale industries: Definition;
Characteristics; Need and rationale.
II Entrepreneurship Opportunity in Agricultural Biotechnology: 12 CO2
Business opportunity, Essential requirement, marketing, strategies,
schemes, challenges and scope-with case study on Plant cell and
tissue culture technique, polyhouse culture. Herbal bulk drug
production, Nutraceuticals, value added herbal products.
Bioethanol production using Agricultural waste, Algal source.
Integration of system biology for agricultural applications.
Biosensor development in Agriculture management.
108
III Entrepreneurship Opportunity in Industrial Biotechnology: 12 CO3

Business opportunity, Essential requirement, marketing strategies,
schemes, challenges, and scope- Pollution monitoring and
Bioremediation for Industrial pollutants. Integrated compost
production- microbe enriched compost. Bio pesticide/ insecticide
production. Biofertilizer. Single cell protein.
IV Therapeutic and Fermented products: Stem cell production, stem 12 CO4
cell bank, production of monoclonal/polyclonal antibodies,
secondary metabolite production – antibiotics, probiotic and
prebiotics.
V Project Management, Technology Management and Startup 12 CO5
Schemes: Building Biotech business challenges in Indian context-
biotech partners (BIRAC, DBT, Incubation centers. etc.,),
operational biotech parks in India. Indian Company act for Bio
business-schemes and subsidies. Project proposal preparation,
Successful start-ups-case study.
Total 60
Course Outcomes
Outcomes
CO1 Describe and apply several entrepreneurial ideas and business PO1, PO2, PO3,
theories in practical framework. PO4, PO5, PO6,
PO7, PO8, PO9,
PO10, PO11, PO12,
PO13, PO14
CO2 Analyse the business environment in order to identify business PO2, PO5, PO7,
opportunities, identify the elements of success of PO8, PO10, PO12,
entrepreneurial ventures, evaluate the effectiveness of different PO14
entrepreneurial strategies and interpret their own business plan.
CO3 Express the mass production of microbial inoculants used as PO4, PO6, PO9,
Biofertilizers and Bioinsecticides in response with field PO11
application and crop response.
CO4 Analyze the application and commercial production of PO5, PO6, PO9,
Monoclonal antibodies, Cytokines. TPH and teaching kits. PO11
CO5 Integrate and apply knowledge of the regulation of PO2,PO7, PO8
biotechnology industries, utilize effective team work skills
within an effective management team with a common
objective, and gain effective team work skills, with an
awareness of cultural diversity and social inclusiveness.
Text Books
109
1. Craig Shimasaki. (2014). Biotechnology Entrepreneurship: Starting, Managing, and Leading

Biotech Companies. Academic Press.
2. Ashton Acton, O. (2012). Biological Pigments– Advances in Research and Application
Scholorly Editions: Atlanta, Georgia.
3. Jennifer Merritt, Jason Feifer (2018). Start Your Own Business, 7th edition, Entrepreneur
Press publisher.
4. Peter F. Drucker(2006). Innovation and Entrepreneurship. Harper Business publisher.
5. Leah Cannon (2017). How to Start a Life Science Company: A Comprehensive Guide for
First-Time Entrepreneurs. International Kindle paperwhite.
References Books
1 Crueger, W, and Crueger. A.(2000). Biotechnology: A Text Book of Industrialmicrobiology,
2nd Edition, Sinauer Associates: Sunderland.Mass.
2 Paul S Teng. (2008). Bioscience Entrepreneurship in AsiaWorld Scientific Publishing
Company.
3 Charles E. Bamford, Garry D. Bruton(2015). ENTREPRENEURSHIP: The Art, Science, and
Process for Success, 2nd Edition, McGraw Hill publisher.
4 Yali Friedman (2014). Building Biotechnology: Biotechnology Business, Regulations,
Patents, Law, Policy and Science 4th Edition, Logos press publication.
5 Stephanie A. Wisner (2022). Building Backwards to Biotech: The Power of Entrepreneurship
to Drive Cutting-Edge Science to Market, International Kindle paperwhite.
Web Resources
https://www.bio-rad.com/webroot/web/pdf/lse/literature/Biobusiness.pdf
1
https://www.crg.eu/biobusiness-entrepreneurship
2
https://www.entrepreneur.com
3
https://www.birac.nic.in
4
https://www.springer.com
5
25 Marks
Evaluation Seminars
External
Evaluation
Total 100 Marks
110
Understand/
Comprehend
overview
(K2)
Analyze (K4)
Evaluate
(K5)
Create (K6)
Presentations

CO1 S S S S S S S S S S S
CO2 S M S S M
CO3
CO4 S S S S
CO5 S S S
111
PROFESSIONAL COMPETENCY SKILL- MICROBIAL QUALITY CONTROL

Subject Subject Categor L T P S Cre Inst. Marks
Code Name y dits Hour CI Exter Tota
s A nal l
MICROBIAL PROFE Y - - - 2 2 25 75 100
QUALITY SSIONA
CONTROL L
AND COMPE
TESTING TENCY
SKILL
Course Objectives
CO1 To understand the use of various advanced techniques for application in the field of
quality control and quality assurance.
CO2 To cultivate skills involved execution of microbiological techniques and to develop the
good laboratory practices.
CO3 To ensure the food safety regulations and its standards.
CO4 To acquire knowledge on laboratory testing, Control & safety process.
CO5 To analyze microbial standards to establish the quality of food products.
Hours Objectives
I Microbial quality control: definition, history and introduction. 12 CO1
Standard Methods involved in assessment of microbial quality
control. Q.A and Q.C definitions and importance. Traditional
Microbiological Quality Controlling methods: Sampling
methods, TVC, APC and serial dilution techniques. Good
laboratory practices, Good microbiological practices.
II Instruments associated in QC & QA: Principle involved, working 12 CO2
conditions, uses and precautions of Laminar Air Flow (LAF),
Autoclave, Incubator, pH meter, Colony counter, Hot air oven,
Centrifuges, colorimeter/ spectrophotometer, ELISA and storage
devices. Methodology of Disinfection, Autoclaving &
Incineration.
III Culture media used in QC and QA: Design of specialized media 12 CO3
for identification of pathogens. Good laboratory practices in
culture media preparation: raw material, water, pH.Uses of
media.Enrichment culture technique, Detection of specific
microorganisms - on XLD agar, Salmonella Shigella Agar,
Mannitol salt agar, EMB agar, McConkey Agar, Saboraud Agar.
IV Determining Microbes in Pharmaceutical Samples: Sterility 12 CO4
112
testing for pharmaceutical products, Bioburden, pyrogen test,

inprocess and final process control, safety and sterility test.
V HACCP for Food Safety and Microbial Standards: Hazard 12 CO5
analysis of critical control point (HACCP) - Principles, flow
diagrams, limitations. Microbial Standards for Different Foods
and Water – BIS standards for common foods and drinking
water.Ascertaining microbial quality of milk by MBRT, Rapid
detection methods of microbiological quality of milk at milk
collection centers.
Total 60
Course Outcomes
Outcomes
CO1 Understand the theoretical assessment of microbial quality PO1, PO5, PO6,
methods and its good laboratory practices. PO9, PO10
CO2 Describe the microbiological aspects of quality control of food PO1, PO4, PO5,
and pharmaceutical products. PO6
CO3 Explain the identification of pathogenic microorganisms and PO1, PO3, PO5,
good laboratory practices. PO6, PO9
CO4 Acquire the knowledge of different sterility test for the PO1, PO4, PO5,
pharmaceutical products. PO6
CO5 Illustrate the safety concern management and regulations of PO1,PO3, PO4,
food and pharmaceutical industry and learn the basic standard PO5, PO6, PO9,
methods and procedures for the microbiological analysis of PO10
food.
Text Books
1 W.B.Hugo&A.D.Russell. (1998). Pharmaceutical Microbiology.6thEdition. Blackwell
scientific Publications.
2 Kulkarni A. K. Bewoor V. A. ()QualityControl,Wiley India Pvt. Ltd,
3 ChandrakantKokare (2016). Pharmaceutical Microbiology, 1st Edition, Nirali Publication.
4 Brown.M.R.W. (2017).Microbiological Quality Assurance
A Guide Towards Relevance and Reproducibility of Inocula,1st Edition. CRC press.
5 Dev Raj Rakesh Sharma And V K Joshi (2011).Quality Control For Value Addition In Food
Processing, New India Publishing Agency.
References Books
1 Rosamund M. Baird, Norman A. Hodges, Stephen P. Denyer. (2000). Handbook of
Microbiological Quality Control in Pharmaceuticals and Medical Devices. 1st Edition, CRC
Press.
2 Konieczka, (2012). Quality Assurance and Quality Control in the Analytical Chemical
113
Laboratory A Practical Approach (Hb), Routledge, Taylor and Francis group.

3 Singh Gajjar, Budhrani, Usman. (2021). Quality Control And Quality Assurance
(M.Pharm)SVikas And Company.
4 David Roesti, Marcel Goverde (2019). Pharmaceutical Microbiological Quality Assurance
and Control: Practical Guide for Non-Sterile Manufacturing, Wiley publication.
5 Amihud Kramer Bernard A. Twigg(2017). Quality Control For The Food Industry
Fundamentals & Applications (Vol.1) 3rd Edition, MEDTEC publication.
Web Resources
1 https://www.study.com/microbiology-quality-control-testing-definition-procedures.
2 https://www.sigmaaldrich.com
3 https://www.coursera.org
4 https://www.atcc.org
5 https://www.fao.org
25 Marks
Evaluation Seminars
External
Evaluation
Total
100 Marks
Understand/ MCQ, True/False, Short essays, Concept explanations, Short summary
Comprehend (K2) or overview
Application (K3)
Observe, Explain
Problem-solving questions, Finish a procedure in many steps,
Analyze (K4)
Differentiate between various ideas, Map knowledge
Check knowledge in specific or offbeat situations, Discussion, Debating
Create (K6)
or Presentations
114

CO/ PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11
PO
CO1 S S S S S
CO2 S M M M
CO3 S M S S M
CO4 S S M M
CO5 S S M S S S S

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APPENDIX - AL

MADURAI KAMARAJ UNIVERSITY

SYLLABUS FOR B.Sc. MICROBIOLOGY

1. Introduction of the programme

2. Eligibility for admission:

2.1 Duration of the programme:

2.2 Medium of Instructions of the programme: English

 To inculcate the basic and advanced concepts of Microbiology including taxonomy,

4. Outcome of the programme:

6a. Subject elective papers

rDNA Technology & Tissue 4 3 3 25 75 100

6b. Skill based subjects

Mushroom Technology 2 2 3 25 75 100

7. Non-Major Elective papers

Basic Microbiology 2 2 3 25 75 100

8. Unitization: Content of every paper divided into FIVE units.

10. Scheme of Internal Evaluation:

The pattern of Internal valuation:

Tests - 10 marks (Average of the best two tests)

Core Practical Internal examination

The pattern for internal valuation for 40 marks may be

11. External Exam : As per the information furnished in No.9.

12. Question Paper Pattern

THEORY QUESTION PAPER PATTERN

Section- A (10 x 1 = 10 Marks) (Answer all the Question; 1 to 10)

1. Choose the correct answer type questions.

Answer all questions – Either Or type

CORE PRACTICAL QUESTION PAPER PATTERN

Major Practical - 1 : 20 Marks

Internal Marks : 40 Marks

13. Scheme for evaluation

14. Passing minimum

To get a pass, a student should fulfill the following conditions:

S.No Range of CCPA Class

15. Model questions

1. discovered Cellular immunity.

2. Administration of antigens is an example of immunity.

3. is an antibody which is abundantly present in breast milk.

4. Two heavy chains of antibodies are joined together by .

6. In Myasthenia Gravis, auto immunity is developed against .

7. Monoclonal antibodies are specific at the level of .

8. In Precipitation antigen is involved.

9. ABO blood grouping was given by .

10. HIV targets cells.

16. Teaching methodology

17. Text books

42) THEORY QUESTION PAPER PATTERN

Section- A (10 x 1 = 10 Marks) (Answer all the Question; 1 to 10)

Choose the correct answer type questions.

Section- B (5 x 7 =35 marks)

Answers not exceeding two

Answer not exceeding four pages (One question from each

a) Two internal test of 15 marks each: Average = 15 marks

CORE PRACTICAL - QUESTION PAPER PATTERN

Time : 6 hours Maximum Marks (University Exam)

- 60 Major Practical - 1 : 20 Marks

Internal Marks : 40 Marks

The pattern for internal valuation for 40 marks may be

19. Retotalling and Revaluation Provision:

20. Transitory provision