B Cells

Antigen BCR

Igα
Igβ

ITAM

Activated B Cell

Germinal Center

B Cell

Follicular
Helper
T Cell

Plasmablast

Memory B Cell

RnDSy-2945
Novus-2945
Plasma Cell
B Cells
B lymphocytes (B cells) are an integral part of the humoral immune response due to their ability to produce antibodies against
foreign antigens. B cells originate from hematopoietic stem cells (HSCs) in the bone marrow, which is seeded during embryonic
development by HSCs from the fetal liver. The initial stages of B cell development are antigen-independent and involve the
generation of several intermediary precursor cells that arise from B lymphocyte progenitor cells including Pre-pro-B cells, Pro-B
cells, and Pre-B cells, which develop into immature B cells. During these stages of development, B cells undergo immunoglobulin
gene rearrangement resulting in the expression of a mature B cell receptor (BCR) that is capable of binding to antigen. This is
followed by a selection process that involves BCR editing or clonal deletion, designed to eliminate self-reactive immature B cells.
The majority of immature B cells that survive this selection process leave the bone marrow and migrate to the spleen where they
differentiate into transitional immature B cells that then become immunocompetent naïve mature B cells. Most naïve B cells
develop into follicular B cells, while a small population becomes marginal zone B cells (frequently called IgM memory B cells in
human). Following antigen-dependent activation, follicular B cells participate in germinal center reactions where they
differentiate into memory B cells or long-lived, antibody-secreting plasma cells. While these developmental stages are similar for
human B cells and mouse conventional B2 cells, a second mouse cell lineage (B1 cells) has also been described. B1 cells are
abundant in the peritoneal cavities and can be further subdivided into B1a and B1b subsets. These along with other B cell
precursor cells and functionally distinct subtypes including marginal zone B cells, follicular B cells, memory B cells, plasma cells,
and regulatory B cells can be distinguished from each other based on the expression of specific cell surface and intracellular
markers. R&D Systems and Novus Biologicals together offer the widest selection of fluorochrome-conjugated antibodies for
detecting human and mouse B cell subsets and further characterizing these cells.

Cell Surface & Intracellular Markers Expressed at Different Stages of B Cell Development
Cell Type Human Markers Mouse Markers
Common Lymphoid
CD10/Neprilysin CD34 Pax5
+ + +
Lin– CD117/c-kit– Sca-1/Ly6– Ly6D– IL-7 Ra+ Flt-3/Flk-2+
Progenitor
B Lymphocyte Progenitor CD10/Neprilysin+ CD34+ Pax5+ Lin– CD117/c-kit– Sca-1/Ly6– Ly6D+ IL-7 Ra+ Flt-3/Flk-2+
Mouse B1 Progenitor Lin– B220/CD45 Rlow CD19+ C1q R1/CD93+
Mouse B2 Progenitor Lin– B220/CD45 R+ CD19– C1q R1/CD93–
Lin– B220/CD45 R+ CD19– CD24low CD43+ C1q R1/CD93+
Pre-Pro B Cell CD117/c-kitlow CD10/Neprilysin+ CD34+ CD38+ Pax5+
CD117/c-kit– CXCR4+ Flt-3/Flk-2+ IL-7 Ra+ IgM–
CD117/c-kitlow CD10/Neprilysin+ CD19+ CD20/MS4A1+ CD24+ CD34+ CD38+
Pro-B Cell Lin– B220/CD45 R+ CD19+ CD24+ CD43+ CD117/c-kitlow IL-7 Ra+ IgM–
C1q R1/CD93+ IL-3 R+ IL-7 Ra+ Pax5+
CD117/c-kit– CD10/Neprilysin+ CD19+ CD20/MS4A1+ CD24+ CD34– CD38+
Pre-B Cell Lin– B220/CD45 R+ CD19+ CD24+ CD43– IL-7 Ra+ IgM–
C1q R1/CD93+ IL-3 R+ IL-4 Ra+ IL-7 Ra+ Pax5+
CD117/c-kit– CD10/Neprilysin+ CD19+ CD20/MS4A1+ CD21+ CD24+ CD27– B220/CD45 R+ CD19+ CD23/Fce RII– CD24+ CD43– C1q R1/CD93+
Immature B Cell
CD38+ CD40+ C1q R1/CD93+ IL-4 Ra+ IL-7 Ra– IgD– IgM+
B1a Cell CD1dmid CD5+ CD19high CD23/Fce RII– CD43+
B1b Cell CD1dmid CD5– CD19high CD23/Fce RII– CD43+

CD10/Neprilysinlow CD5+ CD19+ CD20/MS4A1+ CD21+ CD23/Fce RII+ CD24+ T1: B220/CD45 R+ CD19+ CD24+ CD43– C1q R1/CD93+ IgM+ IgDlow
Transitional B Cell
CD27– CD38+ C1q R1/CD93+ TACI+ T2: B220/CD45 R+ CD19+ CD24+ CD43– C1q R1/CD93+ IgM+ IgD+
B220/CD45 R+ CD1d+ CD19mid CD21high CD23/Fce RII– CD43– C1q R1/
Marginal Zone B Cell CD1c+ CD19+ CD20/MS4A1+ CD21+ CD27+ FCRL3/FcRH3+ TACI+
CD93– IgMhigh IgDlow
CD10/Neprilysin– CD19+ CD20/MS4A1+ CD21+ CD22/Siglec-2+ CD23/Fce B220/CD45 R+ CD1dmid CD19mid CD21low CD23/Fce RII+ CD43– CXCR5+
Follicular B Cell
RII+ CD24low CD27– CD38low CXCR5+ TACI+ MHC class II+ IgMlow IgDhigh
Activated Germinal Center
CD19+ CD20/MS4A1+ CD27+ CD38+ CD40+ CD83+ TACI+ MHC class II+ B220/CD45 R+ CD19+ CD40+ MHC class II+
B Cell
Memory B Cell CD19+ CD20/MS4A1+ CD21+ CD27mid/+ C1q R1/CD93– TACI+ B220/CD45 R+ CD19+ CD21+ CD27mid/+ CD40+ MHC class II+
Plasmablast BCMA+ CD19low CD27high CD38+ C1q R1/CD93+ Syndecan-1/CD138–/low B220/CD45 Rlow CD19+ CD27high CD38+ Syndecan-1/CD138+
BCMA BLIMP1 CD19 CD20/MS4A1
+ + low –/low
CD27 high
CD38 high
Syndecan-1/ B220/CD45 Rlow BLIMP1+ CD19– CD27high, CD38low CXCR4high
Plasma Cell
CD138+ CXCR4+ MHC class IIlow Syndecan-1/CD138+ MHC class II–/low
CD1d+ CD5+ CD19+ CD23/Fce RII–/low CD24+ C1q R1/CD93–/low TIM-1+
Regulatory B Cell CD1d+ CD5+ CD19+ CD21+ CD24+ IL-10+ IL-35+ TGF-b+
IL-10+ IL-35+ TGF-b+

Note: Lin– for Mouse CLP, BLP, B1 and B2 Progenitor cells:

CD3– CD4– CD8– Gr-1/Ly-6G– Integrin aM/CD11b– TER-119–
Lin– for Mouse Pre-Pro B, Pro-B, Pre-B cells: CD3– Gr-1/Ly-
DOWNLOAD NOW
6G– Integrin aM/CD11b– TER-119– Available as a reference tool.
www.rndsystems.com/posters
B Cell Development
Common Lymphoid B Lymphocyte
Progenitor Progenitor Pre-Pro B Cell Pro-B Cell
Multipotent
Progenitor Cell Pre-B Cell

Pro-BCR Igα/β
Hematopoietic Pre-BCR
Igα/β
Stem Cell

Mature
Immature B Cell BCR
Mouse B2
Common Myeloid Mouse B1 Progenitor Igα/β
Progenitor Progenitor
Bone Marrow
Spleen Transitional
B1 Cell
(mouse; from B1 T1 B Cell
Progenitor Cell) (mouse; from B2
B1a BCR Progenitor Cell) BCR
Igα/β Igα/β
T2 B Cell
B1b
BCR T3 B Cell
Peritoneal Cavity Igα/β (human)

Igα/β BCR
Peripherial Blood Naive Mature
B Cell
BCR
Igα/β

Follicular Marginal
B Cell BCR Zone B Cell
Igα/β
Igα/β
Long-lived BCR
Plasma Cell
Igα/β

Activated Antigen Antigen Activated

BCR BCR
B Cell B Cell
Memory B Cell Igα/β

BCR

Plasmablast
Short-lived Plasma Cell

Follicular Helper
T Cells
Activated Germinal
Center B Cell

Lymph Nodes and Germinal Center

Mucosal-Associated Lymphoid Tissue

Memory B Cell

BCR Plasmablast

Long-lived
Plasma Cell

Peripherial Blood
Analysis of Different Stages of B Cell Development in
Mouse Bone Marrow by Flow Cytometry
Common Lymphoid B Lymphocyte
Progenitor Progenitor Pre-Pro B Cell Pro-B Cell
Multipotent
Progenitor Cell Pre-B Cell

Pro-BCR
Iga/b
Hematopoietic Pre-BCR
Iga/b
Stem Cell

Mature
Immature B Cell BCR
Mouse B2 Iga/b
Common Myeloid Mouse B1 Progenitor
Progenitor Progenitor
Bone Marrow

A B C

BLP
CD117/c-kit
Flt-3/Flk-2

Ly6D
CLP

Lineage cocktail Sca-1/Ly6 IL-7 Rα/CD127

Detection of Common Lymphoid Progenitor Cells (CLPs) and B Lymphocyte Progenitor Cells (BLPs) in Mouse Bone Marrow by Flow Cytometry. C57BL/6 mouse bone marrow cells were
depleted of CD3+, CD4+, CD8+, Gr-1+, CD11b/Integrin aM+, TER-119+ cells by staining the cells with Biotinylated Anti-Mouse Monoclonal Antibodies against each of these markers followed
by magnetic depletion using streptavidin-coated magnetic beads. Common lymphoid progenitor cells (CLPs: Lin–/CD117low/Sca-1low/Flt-3high/IL-7 Ra+/Ly-6D–) and B lymphocyte progenitor
cells (BLPs: Lin–/CD117low/Sca-1low/Flt-3high/IL-7 Ra+/Ly-6D+) in the remaining cell population were detected by staining the cells with (A) an Alexa Fluor 405-conjugated lineage cocktail
containing Anti-Mouse Monoclonal Antibodies against each of the depletion markers and a PE-conjugated Rat Anti-Mouse Flt-3/Flk-2 Monoclonal Antibody (R&D Systems, Catalog #
FAB7681P). Lin–/Flt-3+ cells were gated. (B) CD117low/Sca-1low cells in the Lin–/Flt-3+ population were detected using an Alexa Fluor 700-conjugated Rat Anti-Mouse Sca-1/Ly6 Monoclonal
Antibody (R&D Systems, Catalog # FAB1226N) and an APC-conjugated Rat Anti-Mouse CD117/c-kit Monoclonal Antibody (R&D Systems, Catalog # FAB1356A). (C) IL-7 Ra+/Ly-6D– cells
and IL-7 Ra+/Ly-6D+ cells were detected in the Lin–/Flt-3+/CD117mid/low/Sca-1mid/low population using an Alexa Fluor 488-conjugated Rat Anti-Mouse IL-7 Ra/CD127 Monoclonal Antibody
(R&D Systems, Catalog # FAB47742G) and a fluorochrome-conjugated rat anti-mouse Ly-6D monoclonal antibody.

A B C

B1
CD93

CD19

CD19

B1

B2 B2

Lineage cocktail B220/CD45R B220/CD45R

Detection of B1 and B2 Lymphocyte Progenitor Cells in Mouse Bone Marrow by Flow Cytometry. (A) Lin–/CD93+ and Lin–/CD93– cells were detected in C57BL/6 mouse bone marrow
cells by staining with a lineage cocktail containing Alexa Fluor 405-conjugated Anti-Mouse Monoclonal Antibodies against CD3, CD4, CD8, CD11b/Integrin aM, Gr-1, and TER-119 and an
APC-conjugated Rat Anti-Mouse C1q R1/CD93 Monoclonal Antibody (R&D Systems, Catalog # FAB1696A). Lin–/CD93+ cells and Lin–/CD93–cells were gated. (B) B1 (Lin–/CD93+/B220low/
CD19+) and (C) B2 (Lin–/CD93–/B220+/CD19–) progenitor cells were detected by staining with an Alexa Fluor 488-conjugated Rat Anti-Mouse B220/CD45 R Monoclonal Antibody
(R&D Systems, Catalog # FAB1217G) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966).
 Common Lymphoid B Lymphocyte
Progenitor Progenitor Pre-Pro B Cell Pro-B Cell
Multipotent
Progenitor Cell Pre-B Cell

Pro-BCR
Igα/β
Hematopoietic Pre-BCR Igα/β
Stem Cell

Mature
Immature B Cell BCR
Mouse B2
Common Myeloid Iga/b
Mouse B1 Progenitor
Progenitor Progenitor

Bone Marrow

A B
Pro- and Pre-B Cells
B220/CD45R

CD19

Pre-Pro B Cells

Lineage cocktail IgM

C D

Pre-B Cells Pro-B Cells

CD24
CD24

Pre-Pro B Cells

CD43 CD43
Detection of Pre-Pro-B, Pro-B, and Pre-B Cells in Mouse Bone Marrow by Flow Cytometry. (A) Lin–/B220+ cells were detected in C57BL/6 mouse bone marrow by staining with a lineage
cocktail containing Alexa Fluor 405-conjugated Anti-Mouse Monoclonal Antibodies against CD3, CD11b/Integrin aM, Gr-1, and TER-119 and an Alexa Fluor 750-conjugated Rat Anti-
Mouse B220/CD45 R Monoclonal Antibody (R&D Systems, Catalog # FAB1217S). (B) IgM–/CD19– cells (pre-pro-B) and IgM–/CD19+ cells (pro-B and pre-B) were detected in the Lin–/
B220+ population by staining with a PE-Cy7-conjugated Rat Anti-Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940) and a PE-conjugated Rat Anti-Mouse CD19
Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966). (C) Pre-pro-B cells (Lin–/B220+/CD19–/CD24low/CD43+/IgM–), (D) pro-B cells (Lin–/B220+/CD19+/CD24+/CD43+/IgM–)
and pre-B cells (Lin–/B220+/CD19+/CD24+/CD43–/IgM–) were detected by staining with an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals,
Catalog # NBP1-43413AF488) and an APC-conjugated Rat Anti-Mouse CD24 Monoclonal Antibody (R&D Systems, Catalog # FAB8547A).
 Analysis of Different Stages of B Cell Development in
Mouse Spleen by Flow Cytometry
Spleen Transitional
B1 Cell
(mouse; from B1 T1 B Cell
Progenitor Cell) (mouse; from B2
B1a BCR Progenitor Cell) BCR
Igα/β Igα/β
T2 B Cell
B1b
BCR T3 B Cell
Peritoneal Cavity Igα/β (human)

Iga/b BCR
Peripherial Blood Naive Mature
B Cell
BCR
Iga/b

A B C
T1 T2
Relative Cell Number

CD43

IgM
CD19 B220/CD45R IgD
Detection of Transitional 1 (T1) and Transitional 2 (T2) B Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with a PE-conjugated Rat Anti-Mouse CD19
Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-24966) and CD19+ cells were gated. (B) B220+/CD43– cells in the CD19+ cell population were detected by staining with an Alexa
Fluor 750-conjugated Rat Anti-Mouse B220/CD45 R Monoclonal Antibody (R&D Systems, Catalog # FAB1217S) and an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal
Antibody (Novus Biologicals, Catalog # NBP1-43413AF488). (C) Transitional 1 (T1) B cells (CD19+/B220+/CD43-/IgM+/IgDlow) and transitional 2 (T2) B cells (CD19+/B220+/CD43-/IgM+/
IgD+) were detected in the CD19+/B220+/CD43- population by staining with an Alexa Fluor 647-conjugated rat anti-mouse IgD monoclonal antibody and a PE-Cy7-conjugated Rat Anti-
Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940).

A B
CD23

CD5

B1a

B1b

CD43 CD19
Detection of Transitional B1a and B1b Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal
Antibody (Novus Biologicals, Catalog # NBP1-43413AF488) and an Alexa Fluor 594-conjugated Rat Anti-Mouse CD23/Fce RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T).
CD43+/CD23– cells were gated. CD1dmid cells in the CD43+/CD23– population were detected by staining with an Alexa Fluor 700-conjugated Rat Anti-Mouse CD1d Monoclonal Antibody
(Novus Biologicals, Catalog # NBP1-43461AF700; Data not shown). (B) Transitional B1a cells (CD5+/CD19high/CD1dmid/CD23–/CD43+) and transitional B1b cells (CD5–/CD19high/CD1dmid/
CD23–/CD43+) were detected in the CD1dmid/CD23-/CD43+ population by staining with a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-
24966) and an APC-conjugated Rat Anti-Mouse CD5 Monoclonal Antibody (R&D Systems, Catalog # FAB115A).
 Iga/b (human)
Spleen

Iga/b BCR
Peripherial Blood Naive Mature
B Cell
BCR
Igα/β

Follicular Marginal
B Cell BCR Zone B Cell
Igα/β
Igα/β
Long-lived BCR
Plasma Cell

A B C
▀ MZ B ▀ FO B

MZ B Cell

CD23
CD43

CD1d

Follicular B Cell

CD19 CD21 CD21

Detection of Marginal Zone and Follicular B-2 Cells in Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody
(Novus Biologicals, Catalog # NBP2-24966) and an Alexa Fluor 488-conjugated Rat Anti-Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488). CD19+/
CD43– cells were gated. (B) Follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43–) and marginal zone B-2 cells (CD19mid/CD1dhigh/CD23–/CD21high/CD43–) were detected in the
CD19+/CD43– population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor 700-conjugated Rat Anti-Mouse CD1d Monoclonal
Antibody (Novus Biologicals, Catalog # NBP1-43461AF700). CD1dmid/CD21low and CD1dhigh/CD21high cells were gated. (C) Follicular B-2 cells (CD1dmid/CD21low) and marginal zone B-2 cells
(CD1dhigh/CD21high) were stained for CD21 and CD23 using a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor 594-conjugated Rat Anti-Mouse CD23/
Fce RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T).

A B C
▀ MZ B ▀ FO B

Follicular B Cell
CD23
CD19

IgM

MZ
B Cell

CD43 CD21 IgD

Detection of IgD and IgM on Marginal Zone and Follicular B-2 Cells from Mouse Splenocytes. (A) C57BL/6 mouse splenocytes were stained with an Alexa Fluor 488-conjugated Rat Anti-
Mouse CD43 Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-43413AF488) and a PE-conjugated Rat Anti-Mouse CD19 Monoclonal Antibody (Novus Biologicals, Catalog #
NBP2-24966). CD19mid/CD43– cells were gated. (B) Follicular B-2 cells (CD19mid/CD1dmid/CD23+/CD21low/CD43–) and marginal zone B-2 cells (CD19mid/CD1dhigh/CD23–/CD21high/
CD43–) were detected in CD19+/CD43– population by staining with a fluorochrome-conjugated anti-mouse CD21 monoclonal antibody and an Alexa Fluor 594-conjugated Rat Anti-Mouse
CD23/Fce RII Monoclonal Antibody (R&D Systems, Catalog # FAB6900T). CD21low/CD23+ and CD21high/CD23– cells were gated. (C) Expression of IgM and IgD on follicular B-2 (CD19mid/
CD23+/CD21low/CD43–/IgMlow/IgDhigh) and marginal zone B-2 (CD19mid/CD23–/CD21high/CD43–/IgMhigh/IgDlow) cells was detected using an Alexa Fluor 647-conjugated rat anti-mouse IgD
monoclonal antibody and a PE-Cy7-conjugated Rat Anti-Mouse IgM Monoclonal Antibody (Novus Biologicals, Catalog # NBP1-42940).
Analysis of Memory B Cells and Plasmablasts in
Mouse Spleen by Flow Cytometry
Iga/b
Spleen
Long-lived
Plasma Cell Activated Antigen Antigen Activated
BCR BCR
B Cell B Cell

Iga/b
Memory B Cell

Short-lived
Plasma Cell

Follicular Helper
Plasmablast T Cells Activated Germinal
Center B Cell
Germinal Center

A B Detection of Memory B Cells and Plasmablasts in Mouse

Splenocytes. (A) Memory B cells (CD27+/CD19+) from
immunized mouse Balb/c splenocytes were detected by
Memory B Cell staining with an Alexa Fluor 488-conjugated Rat Anti-
Plasmablast Mouse CD27/TNFRSF7 Monoclonal Antibody (Novus
Biologicals, Catalog # NBP2-11950AF488) and a PE-
conjugated Rat Anti-Mouse CD19 Monoclonal Antibody
CD138

(Novus Biologicals, Catalog # NBP2-24966). CD27+/CD19+

CD19

cells were gated. (B) Plasmablasts (CD19+/CD27+/CD38+/

CD138+) were detected in the CD27+/CD19+ population by
staining with an Alexa Fluor 700-conjugated Rat Anti-
Mouse CD38 Monoclonal Antibody (Novus Biologicals,
Catalog # NB100-77405AF700) and an APC-conjugated
Rat Anti-Mouse CD138/Syndecan-1 Monoclonal Antibody
(R&D Systems, Catalog # FAB2966A).

CD27 CD38

Analysis of Follicular Helper T Cells in Mouse Spleen

B
A
A B
CXCR5

Rat IgG2A

Tfh

CD4 CD4
Detection of T Follicular Helper (Tfh) Cells in Mouse Splenocytes. (A) Immunized Balb/c mouse splenocytes were stained
an Alexa Fluor 405-conjugated Rat Anti-Mouse CD4 Monoclonal Antibody (R&D Systems, Catalog # FAB554V) and either
(A) an APC-conjugated Rat Anti-Mouse CXCR5 Monoclonal Antibody (Catalog # FAB6198A) or (B) an APC-conjugated Rat
IgG2A Isotype Control (Catalog # IC006A).
Analysis of Human Plasma Cells and Memory B Cells by Flow Cytometry
Iga/b
Spleen
Long-lived
Plasma Cell Activated Antigen Antigen Activated
BCR BCR
B Cell B Cell

Iga/b
Memory B Cell

Short-lived
Plasma Cell

Follicular Helper
Plasmablast T Cells Activated Germinal
Center B Cell
Germinal Center

A B C

Plasma Cells
Plasma Cells

BLIMP1
CD38
CD19

CD3 CD20 CD38

Detection of Plasma Cells and Memory B Cells in Human Peripheral Blood Mononuclear Cells by Flow Cytometry. Plasma
cells (CD3–/CD19low/CD20–/low/CD38high/BLIMP1+) in human peripheral blood mononuclear cells were detected by staining
Memory B Cells with (A) an Alexa Fluor 405-conjugated Mouse Anti-Human CD3e Monoclonal Antibody (R&D Systems, Catalog # FAB100V)
and an Alexa Fluor 594-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867T).
CD19

CD3–/CD19+ cells were gated. (B) Expression of CD20/MS4A1 and CD38 on cells in the CD3–/CD19+ gate was determined
by staining with an APC-conjugated Mouse Anti-Human CD20/MS4A1 Monoclonal Antibody (R&D Systems, Catalog #
FAB4225A) and a PerCP-conjugated Mouse Anti-Human CD38 Monoclonal Antibody (R&D Systems, Catalog # FAB2404C).
CD20–/low/CD38+ cells were gated. (C) Expression of BLIMP1 in the CD20–/low/CD38+ cell population was determined by
staining with a PerCP-conjugated Mouse Anti-Human CD38 Monoclonal Antibody (R&D Systems, Catalog # FAB2404C) and
a PE-conjugated Mouse Anti-Human BLIMP1/PRDM1 Monoclonal Antibody (R&D Systems, Catalog # IC36081P). (D)
Memory B cells were also detected in the starting population of human peripheral blood mononuclear cells by staining with
a Fluorescein-conjugated Mouse Anti-Human CD27/TNFRSF7 Monoclonal Antibody (R&D Systems, Catalog # FAB382F)
and an Alexa Fluor 594-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (R&D Systems, Catalog # FAB4867T).

CD27

Detection of Pax5 Expression in Human B Cells by Flow

Pax5

Cytometry. Human peripheral blood mononuclear cells

were surface stained using an APC-conjugated Mouse Anti-
Human CD19 Monoclonal Antibody (R&D Systems, Catalog
# FAB4867A). Cells were then fixed and permeabilized and
stained intracellularly using a Rabbit Anti-Human Pax5
Monoclonal Antibody (R&D Systems, coming soon)
followed by a PE-conjugated Goat Anti-Rabbit Antibody
(R&D Systems, Catalog # F0110).

CD19
Fluorochrome-conjugated Antibodies for Detecting Different Stages of
Human and Mouse B Cell Development
Antibodies for B Cell Lineage Negative Markers used to Enrich for B Cell Progenitors
Fluorochrome-conjugated Antibodies for Flow Cytometry (Catalog #s)

Additional Alexa Fluor Unconjugated

Molecule Species Clone Alexa Fluor
APC Fluorescein PE PerCP conjugates Biotin Antibodies
488 700 350/405/594/647/750 (Applications)

FAB100V/FAB100T/FAB100R/ MAB100 (FA, FC,

Human UCHT1 FAB100A FAB100F FAB100P FAB100C FAB100G FAB100N
FAB100S ICC/IF, IP)

FAB4841V/FAB4841T/ MAB4841 (FA, FC,

Mouse 17A2 FAB4841A FAB4841F FAB4841P FAB4841C FAB4841G FAB4841N BAM4841
CD3 FAB4841R/FAB4841S ICC/IF, IHC, IP)

NBP2-30151 (FC);
NBP2- NBP2- NBP2- FAB484U/FAB484V/FAB484T/
Mouse 145-2C11 FAB484G FAB484N NBP2-30149B MAB484 (Depl, FA,
30149APC 30149PE 30149PCP FAB484R/FAB484S
FC, IP)

Human 11830 FAB3791A FAB3791F FAB3791P FAB3791C FAB3791G FAB3791N

NBP2- NBP2- NBP2- NBP2-27216AF405/ NBP2-25199 (B/N,

Human RPA-T4 NBP2-27245 NBP2-27247 NBP2-27248
CD4 27216PCP 27216AF488 27216AF700 NBP2-27216AF647 FC, IHC, IV)

FAB554V/FAB554T/FAB554R/ MAB554 (Depl, FA,

Mouse GK1.5 FAB554A FAB554F FAB554P FAB554C FAB554G FAB554N BAM554
FAB554S FC, IHC, IP)

FAB1509V/FAB1509T/ MAB1509 (FC,

Human 37006 FAB1509A FAB1509F FAB1509P FAB1509C FAB1509G FAB1509N
FAB1509R/FAB1509S ICC/IF)

NBP2- NBP2- NBP2- NBP2- NBP2- NBP2-34588AF405/ NBP2-32836 (FC,

Human C8/144B NBP2-34588B
34588APC 34588PE 34588PCP 34588AF488 34588AF700 NBP2-34588AF647 ICC/IF, IHC, IP, WB)
CD8a
NBP2- NBP2- NBP2- NBP2- NBP2-25195AF405/ NBP2-25195 (FC,
Human RPA-T8 NBP2-27246 NBP2-27237
27235 25195PCP 25195AF488 25195AF700 NBP2-25195AF647 IHC, IV)

FAB116V/FAB116T/FAB116R/ MAB116 (Depl, FA,

Mouse 53-6.7 FAB116A FAB116F FAB116P FAB116C FAB116G BAM116
FAB116S FC, ICC/IF, IP)

MAB1699 (FC, ICC/IF,

ICRF44 FAB1699A FAB1699P FAB1699G BAM1699
IHC)
Human
Integrin FAB16991V/FAB16991T/ MAB16991 (FC,
238446 FAB16991A FAB16991P FAB16991C FAB16991G FAB16991N
aM/CD11b FAB16991R/FAB16991S ICC/IF)

FAB1124V/FAB1124T/ MAB1124 (FC, ICC/IF,

Mouse M1/70 FAB1124A FAB1124F FAB1124P FAB1124C FAB1124N BAM1124
FAB1124R/FAB1124S IHC, IP)

MAB1037 (FC, ICC/IF,

Gr-1/Ly-6G Mouse RB6-8C5 FAB1037A FAB1037F FAB1037P FAB1037C FAB1037N FAB1037V BAM1037
IHC, IP)

MAB1125 (FC, IHC,

TER-119 Mouse TER-119 FAB1125A FAB1125F FAB1125P FAB1125N FAB1125V BAM1125
IP, WB)

Antibodies for Select Markers used to Identify B Cell Subsets by Flow Cytometry
Fluorochrome-conjugated Antibodies for Flow Cytometry (Catalog #s)

Additional Alexa Fluor Unconjugated

Molecule Species Clone Alexa Fluor
APC Fluorescein PE PerCP conjugates Antibodies
488 700 405/594/647/750 (Applications)

FAB1217V/FAB1217T/ MAB1217 (FC, ICC/IF,

B220/CD45 R Mouse RA3-6B2 FAB1217A FAB1217F FAB1217P FAB1217C FAB1217G FAB1217N
FAB1217R/FAB1217S IP)

MAB140 (B/N, E, FC,

Human 37711 FAB140F FAB140P
IHC)
B7-1/CD80
NBP1-43385 (FA, FC,
Mouse 16-10A1 NBP1-43030 NBP1-43990 NBP1-43842
IHC, IP)

MAB141 (B/N, FC,

Human 37301 FAB141A FAB141F FAB141P FAB141C FAB141N FAB141T/FAB141R
WB)
B7-2/CD86
MAB741 (B/N, FC,
Mouse GL1 FAB741A FAB741P FAB741C FAB741G
WB)

AF1162 (B/N, FC,

BAFF R/ Human Polyclonal FAB1162A FAB1162P
WB)
TNFRSF13C
Mouse 204406 FAB1755F MAB1755 (FC)

BCMA/ AF193 (B/N, E, FC,

Human Polyclonal FAB193A FAB193F FAB193P FAB193N
TNFRSF17 WB)

Human 646702 IC36081A IC36081P IC36081G IC36081N IC36081R

BLIMP1/
PRDM1 NB600- NB600- NB600- NB600- NB600-235AF405/ NB600-235 (ChIP, E,
Human/Mouse 3H2-E8 NB600-235F NB600-235PE
235APC 235PCP 235AF488 235AF700 NB600-235AF647 FC, ICC/IF, IHC, WB)

Human 273107 FAB23791A MAB23791 (FC, WB)

C1q R1/CD93
Mouse 223437 FAB1696A FAB1696P MAB1696 (FC, ICC/IF)

CD1c Human Polyclonal FAB5910A FAB5910P

Application Key: B/N Blocking/Neutralization ChiP Chromatin Immunoprecipitation Depl Depletion E ELISA FA Functional Assay FC Flow Cytometry ▀ R&D Systems product ▀ Novus Biologicals product
ICC/IF Immunocytochemistry/Immunofluorescence IHC Immunohistochemistry IP Immunoprecipitation IV In vitro WB Western Blot
Antibodies for Select Markers used to Identify B Cell Subsets by Flow Cytometry
Fluorochrome-conjugated Antibodies for Flow Cytometry (Catalog #s)

Additional Alexa Fluor Unconjugated

Molecule Species Clone Alexa Fluor
APC Fluorescein PE PerCP conjugates Antibodies
488 700 405/594/647/750 (Applications)

NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-43460AF405/ NBP1-43460 (FC,

Human 51.1
43460APC 43460PE 43460PCP 43460AF488 43460AF700 NBP1-43460AF647 IHC, IP)
CD1d
NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-43461AF405/ NBP1-43461 (FC,
Mouse 1B1
43461APC 43461PE 43461PCP 43461AF488 43461AF700 NBP1-43461AF647 IHC, IP)

MAB1636 (FA, FC,

Human 205919 FAB1636P
WB)
CD5
MAB115 (FA, FC, IHC,
Mouse 53-7.3 FAB115A FAB115F FAB115P FAB115N FAB115V
IP)

Neprilysin/ NB100- NB100- NB100- NB100- NB100- NB100-77917AF405/ NB100-77917 (FC,

Human HI10a NB100-77918
CD10 77917APC 77917PE 77917PCP 77917AF488 77917AF700 NB100-77917AF647 WB)

FAB4867T/FAB4867R/
Human 4G7-2E3 FAB4867A FAB4867F FAB4867P FAB4867C FAB4867N MAB4867 (FC)
FAB4867S

NB500- NB500- NB500- NB500- NB500-338AF405/

LT19 NB100-63513 NB500-338PE NB500-338 (FC, IP)
338APC 338PCP 338AF488 338AF700 NB500-338AF647

NBP2- NBP2- NBP2- NBP2- NBP2-25196AF405/ NBP2-25196 (FC,

CB19 NBP2-26643 NBP2-26646
25196APC 25196PCP 25196AF488 25196AF700 NBP2-25196AF647 ICC/IF, IHC, IV, WB)
CD19 Human
NBP1-50058 (FC,
4G7 NBP1-79128 NBP1-79129
ICC/IF)

NBP1-28375 (FC,
SJ25-C1 NBP1-28379 NBP1-28378
IHC, IP)

NBP2- NBP2- NBP2- NBP2-24965AF405/

Mouse 1D3 NBP2-24968 NBP2-24967 NBP2-24966 NBP2-24965 (FC)
24965PCP 24965AF488 24965AF700 NBP2-24965AF647

Human 396444 FAB4225A FAB4225F FAB4225P FAB4225N FAB4225V MAB4225 (FC)

NB100- NB100- NB100- NB100- NB100- NB100-64858AF405/ NB100-64858 (FC,

Human 2H7 NB100-63542
CD20/MS4A1 64858APC 64858PE 64858PCP 64858AF488 64858AF700 NB100-64858AF647 IHC, IP)

NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-43435AF405/ NBP1-43435 (FC,

Mouse AISB12
43435APC 43435PE 43435PCP 43435AF488 43435AF700 NBP1-43435AF647 WB)

CD21 Human 544408 FAB4909A FAB4909F FAB4909P MAB4909 (FC, WB)

MAB123 (B/N, FC,

Human 138628 FAB123P
WB)
CD23/Fce RII
Mouse 691632 FAB6900A FAB6900P FAB6900T MAB6900 (FC)

Mouse B3B4 NB100-77387 NB100-63615

Human ML5 FAB5247A

NB100- NB100- NB100- NB100- NB100- NB100-77388AF405/

CD24 Human/Mouse M1/69 NBP2-00007 NB100-77388 (FC)
77388APC 77388PE 77388PCP 77388AF488 77388AF700 NB100-77388AF647

Mouse 910019 FAB8547A FAB8547P FAB8547G MAB8547 (FC, IHC)

Human 24212 FAB1020A FAB1020P FAB1020G MAB1020 (FC, WB)

Human BC96 NBP1-43049 NB100-77772 NBP1-43879 NBP1-43430 (FC)

Mouse 280406 FAB2438A FAB2438P FAB2438C FAB2438G MAB2438 (FC)

CD25/IL-2 Ra
NBP2- NBP2- NBP2- NBP2-27425AF405/
Mouse PC61 NBP2-30135 NBP2-30134 NBP2-27426 NBP2-27425 (FC)
27425PCP 27425AF488 27425AF700 NBP2-27425AF647

Mouse 7D4 NBP1-27921 NBP1-27920

MAB382 (B/N, FC,

Human 57703 FAB382A FAB382F FAB382P
WB)

NBP1-43427 (FC,
Human/Mouse LG.3A10 NBP1-50436
CD27 IHC, IP)

Human/ NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-43428AF405/ NBP1-43428 (FA, FC,
LG.7F9 NBP1-44021
Mouse/Rat 43428APC 43428PE 43428PCP 43428AF488 43428AF700 NBP1-43428AF647 IP)

Mouse 137915 FAB5741A FAB5741P MAB5741 (E, FC)

CD30 Human 81337 FAB229F FAB229P MAB229 (FA, FC, WB)

Human QBEnd10 FAB7227A FAB7227P FAB7227G

CD34 MAB72271 (FC, ICC/
Human 756510 FAB72271P FAB72271G
IF)

MAB2404 (FC, ICC/IF,

Human 240742 FAB2404A FAB2404F FAB2404P FAB2404C
IP)

NB500- NB500- NB500-510AF405/ NB500-510 (FC, IHC,

CD38 Human HIT2 NB110-55355 NB500-592 NBP1-44720 NBP1-44723
510AF488 510AF700 NB500-510AF647 WB)

NB100- NB100- NB100- NB100- NB100- NB100-77405AF405/

Mouse 90 NBP1-27957 NB100-77405 (FC)
77405APC 77405PE 77405PCP 77405AF488 77405AF700 NB100-77405AF647
Fluorochrome-conjugated Antibodies for Detecting Different Stages of
Human and Mouse B Cell Development continued

Antibodies for Select Markers used to Identify B Cell Subsets by Flow Cytometry
Fluorochrome-conjugated Antibodies for Flow Cytometry (Catalog #s)

Additional Alexa Fluor Unconjugated

Molecule Species Clone Alexa Fluor
APC Fluorescein PE PerCP conjugates Antibodies
488 700 405/594/647/750 (Applications)

MAB6321 (FA, FC,

Human 82111 FAB6321A FAB6321P
CD40/ ICC/IF)
TNFRSF5 NBP1-43416 (FA, FC,
Human 5C3 NBP1-44013 NBP1-43869
IHC)

Human 290111 FAB2038A FAB2038P MAB2038 (FC, ICC/IF)

NBP2- NBP2- NBP2- NBP2- NBP2-33140AF405/ NBP2-15190 (E, FC,

Human DF-T1 NBP2-33140PE
CD43 33140APC 33140PCP 33140AF488 33140AF700 NBP2-33140AF647 ICC/IF, IHC, IP, WB)

NBP1- NBP1- NBP1- NBP1- NBP1-43413AF405/ NBP1-43413 (FC, IP,

Mouse R2/60 NBP1-44011 NBP1-43413PE
43413APC 43413PCP 43413AF488 43413AF700 NBP1-43413AF647 WB)

MAB332 (B/N, E, FC,

Human 47233 FAB332A FAB332P FAB332C
WB)

NB100- NB100- NB100- NB100- NB100- NB100-77477AF405/ NB100-77477 (FC,

Human/Mouse 2B8 NBP1-43974
77477APC 77477PE 77477PCP 77477AF488 77477AF700 NB100-77477AF647 IHC, IP)
CD117/c-kit
NB600- NB600- NB600- NB600- NB600-765AF405/ NB600-765 (FC, ICC/
Human/Mouse 104D2 NB600-765PE
765APC 765PCP 765AF488 765AF700 NB600-765AF647 IF)

MAB1356 (FC, IHC,

Mouse 180627 FAB1356A FAB1356P
WB)

Human 12G5 FAB170A FAB170F FAB170P FAB170C FAB170G FAB170N MAB170 (B/N)

Human 44717 FAB173A FAB173P FAB173C FAB173G FAB173N MAB173 (B/N, FC)
CXCR4
MAB21651 (B/N, FC,
Mouse 247506 FAB21651A FAB21651F FAB21651P FAB21651C
ICC/IF, IHC)

MAB190 (B/N, FC,

Human 51505 FAB190A FAB190F FAB190P FAB190C FAB190N
CXCR5 ICC/IF, IHC)

Mouse 614641 FAB6198A FAB6198F FAB6198P FAB6198C MAB6198 (FC, ICC/IF)

FCRL3/FcRH3 Human 546828 FAB3126A FAB3126P FAB3126G MAB3126 (FC)

Flt-3/Flk-2/ Human 66903 FAB812A FAB812F FAB812P FAB812N MAB812 (FC)

CD135 Mouse 113308 FAB7681A FAB7681P MAB7681 (FC, ICC/IF)

FAB4869V/FAB4869T/
Human L203 FAB4869A FAB4869F FAB4869P FAB4869C FAB4869N MAB4869 (FC)
FAB4869R/FAB4869S
HLA-DR
NB100- NB100- NB100- NB100- NB100- NB100-77855AF405/ NB100-77855
Human L243 NB100-77856
77855APC 77855PE 77855PCP 77855AF488 77855AF700 NB100-77855AF647 (FC, IHC, IP, WB)

HVEM/
Human 94801 FAB356A MAB356 (E, FC, WB)
TNFRSF14

Mouse 11-26c NBP1-43937 NBP1-43732 NBP1-43258 (FC)

IgD NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-48638AF405/
Mouse AMS-9.1 NBP2-29913 NBP1-48638 (FC)
48638APC 48638PE 48638PCP 48638AF488 48638AF700 NBP1-48638AF647

NBP1-43303 (E, FC,

IgM Mouse II/41 NBP1-42995 NBP1-43947 NBP1-43763
IHC)

MAB301 (B/N, FC,

Human 32703 FAB301A FAB301P FAB301C FAB301G FAB301N
ICC/IF, IHC, WB)
IL-3 Ra
NB600- NB600- NB600- NB600- NB600- NB600-1185AF405/ NB600-1185 (FC,
Human 6H6 NB100-77827
1185APC 1185PE 1185PCP 1185AF488 1185AF700 NB600-1185AF647 IHC, WB)

MAB230 (B/N, FC,

IL-4 Ra Human 25463 FAB230A FAB230F FAB230P FAB230C FAB230N
IHC, WB)

Human 40131 FAB306A FAB306P FAB306C FAB306G FAB306N MAB306 (FC, WB)
IL-7 Ra/CD127
Mouse A7R34 FAB47742A FAB47742P FAB47742G FAB47742N

Human 127107 IC2172F IC2172P

NBP2-27574 (E, FC,

Human JES3-9D7
IL-10 WB)

NBP1- NBP1- NBP1- NBP1- NBP1- NBP1-06673AF405/

Mouse AP-MAB0851 NBP1-06673 (FC, IP)
06673APC 06673PE 06673PCP 06673AF488 06673AF700 NBP1-06673AF647

Application Key: B/N Blocking/Neutralization ChiP Chromatin Immunoprecipitation Depl Depletion E ELISA FA Functional Assay FC Flow Cytometry ▀ R&D Systems product ▀ Novus Biologicals product
ICC/IF Immunocytochemistry/Immunofluorescence IHC Immunohistochemistry IP Immunoprecipitation IV In vitro WB Western Blot
Antibodies for Select Markers used to Identify B Cell Subsets by Flow Cytometry
Fluorochrome-conjugated Antibodies for Flow Cytometry (Catalog #s)

Additional Alexa Fluor Unconjugated

Molecule Species Clone Alexa Fluor
APC Fluorescein PE PerCP conjugates Antibodies
488 700 405/594/647/750 (Applications)

NBP2- NBP2-34848 (FC,

Human CVS20
MHC class II 34848AF488 IHC, IP)

Mouse M5/114.15.2 FAB6118A FAB6118F

TACI/ Human 165604 FAB1741A FAB1741P FAB1741C FAB1741G MAB1741 (B/N, FC)
TNFRSF13B Mouse 166010 FAB1041A FAB1041P MAB1041 (E, FC, WB)

MAB240 (B/N, E, FC,

Human 9016 IC240A IC240F IC240P
TGF-b1 IHC, WB)

Mouse 860206 MAB7666 (FC)

TGF-b1, 2, 3 Multi-species 1D11 IC1835A IC1835P IC1835N

Pax5 Human/Mouse 1H9 NBP2-00257

Sca-1/Ly6 Mouse 177228 FAB1226A FAB1226P FAB1226G FAB1226N MAB1226 (FC, ICC/IF)

Mouse 95218 FAB5761F FAB5761P MAB5761 (FC)

L-Selectin/
CD62L NBP2-00260 (FC,
Mouse MEL-14 NBP1-28010 NBP1-28007 NB100-63971
IHC, IP)

Human 219934 FAB1968F FAB1968P MAB1968 (FC, ICC/IF)

Siglec-2/CD22
Mouse 308501 FAB2296A FAB2296F FAB2296P MAB2296 (FC, WB)

Siglec-G Mouse 805903 FAB7775A MAB7775 (FC)

Human 359103 FAB2780A FAB2780F FAB2780P MAB2780 (FC, IHC)

Syndecan-1/ NB100-64980 (FA,

Human B-A38 NB100-63417 NB110-81751 NBP1-57898
CD138 FC, ICC/IF, IHC, WB)

Mouse 300506 FAB2966A FAB2966F FAB2966P MAB2966 (FC, ICC/IF)

Learn more | rndsystems.com/bcells and novusbio.com/b-cell-research

Additional Products for B Cell Research
In addition to the large selection of fluorochrome-conjugated antibodies that are offered by R&D Systems and Novus Biologicals, R&D Systems
offers several kits to simplify your B cell research including B Cell Isolation and Expansion Kits, and recombinant proteins for B cell culture and
differentiation. We also offer a wide range of assays designed to provide insight into the B cell-mediated immune response such as B Cell
ELISpot Development Modules, Luminex® Immunoglobulin Isotyping Assays, and ELISA Kits to quantify B cell-secreted molecules.

B Cell Isolation Kits

R&D Systems® MagCellect™ B Cell Isolation Kits are designed to isolate B cells from a mononuclear cell suspension by negative selection.
Using these kits, unwanted cells are magnetically tagged with a biotinylated antibody cocktail and streptavidin ferrofluid. The cell suspension is
subsequently placed in a magnetic field, and the desired, untouched B cell population is harvested by aspiration and immediately available for
a variety of downstream applications.

B Cell Isolation Kits

Kit Catalog #
MagCellect™ Human B Cell Isolation Kit MAGH103
MagCellect™ Mouse B Cell Isolation Kit MAGM204
MagCellect™ Rat B Cell Isolation Kit MAGR303

A 104
0.17%
B 104
0.40% 0.31%

22.74%
103 103
CD14

CD14

102 102
68.53%

101 101

8.56% 2.38% 96.91%

100 100
100 101 102 103 104 100 101 102 103 104
CD19 CD19
Enrichment of Human B Cells using the MagCellect Human B Cell Isolation Kit. Ficolled human peripheral blood
mononuclear cells before (A) and after (B) isolation of B cells using the MagCellect Human B Cell Isolation Kit (Catalog #
MAGH103). Dot plots reflect double-staining of all viable cells using a Fluorescein-conjugated Mouse Anti-Human CD19
Monoclonal Antibody (R&D Systems, Catalog # FAB4867F) and a PE-conjugated Mouse Anti-Human CD14 Monoclonal
Antibody (R&D Systems, Catalog # FAB3832P).

Learn more | rndsystems.com/magcellect

Luminex Mouse Immunoglobulin Isotyping Assay

®

This bead-based assay is designed to simultaneously quantify multiple mouse immunoglobulin isotype concentrations
in cell culture supernatants, serum, or EDTA plasma. The assay is designed for use with the Luminex MAGPIX®,
Luminex 100™, Luminex 200™, or Bio-Rad® Bio-Plex® dual laser, flow-based sorting and detection analyzers.
Luminex® Immunoglobulin Isotyping Assay
Analyte Polystyrene Beads Magnetic Beads
Catalog # Catalog #
Mouse IgG1 LXIGM1 LXMIGMG1
Mouse IgG2A LXIGM2 LXMIGMG2
Mouse IgG2B LXIGM3 LXMIGMG3
Mouse IgG3 LXIGM4 LXMIGMG4
Mouse IgA LXIGM5 LXMIGMG5
Mouse IgM LXIGM6 LXMIGMG6
Mouse Immunoglobulin Isotyping Base Kit LXIGM LXMIGMG

Learn more | rndsystems.com/luminex

B Cell Expansion Kit
R&D Systems® CellXVivo™ Human B Cell Expansion Kit contains all of the reagents necessary
to expand a population of 107 human B cells 3–5-fold.

B Cell Expansion Kit

Kit Catalog #
CellXVivo™ Human B Cell Expansion Kit CDK005

50
4500

4000
40
3500

Relative Cell Number

3000
30
Mean RFU

2500

2000

1500
20

1000
10
500
0
Cells Cultured with Basal Media
B Cell Expansion Kit 0
100 101 102 103 104
CD20
Expansion of B Cells using the CellXVivo™ Human B Cell Detection of CD20 in Human B Cells. Human B cells were
Expansion Kit. Human B cells were isolated from peripheral expanded for 5 days using reagents included in the Human
blood mononuclear cells and cultured for five days using B Cell Expansion Kit. The cells were labeled with a PE-
reagents included in the CellXVivo™ Human B Cell conjugated Mouse Anti-Human CD20 Monoclonal Antibody
Expansion Kit (R&D Systems, Catalog # CDK005). B cell (R&D Systems, Catalog # FAB4225P; filled histogram) or a
expansion was measured with Resazurin (R&D Systems, PE-conjugated Mouse IgG1 Isotype control (R&D Systems,
Catalog # AR002). Catalog # IC002P; open histogram).

Learn more | rndsystems.com/cellxvivo

Recombinant Proteins
R&D Systems is the most referenced manufacturer of recombinant and natural proteins in the
scientific literature. By maintaining stringent production and purification standards, we ensure
that our proteins provide researchers with industry-leading bioactivity and lot-to-lot
consistency. In addition to the more than 4,800 proteins that we manufacture under standard
conditions, we also offer Animal-Free™ and GMP-grade recombinant proteins as well as
custom protein development services.

Select Recombinant Proteins for B Cell Culture & Differentiation

Molecule Species Catalog #
Human 6420-CL
CD40 Ligand
Mouse 8230-CL
Human 202-IL
IL-2
Mouse 402-IL
Human 204-IL
IL-4
Mouse 404-ML
Human 206-IL
IL-6
Mouse 406-ML
Human 217-IL
IL-10
Mouse 417-ML
Human 8879-IL
IL-21
Mouse 594-ML
Recombinant Human IL-2, IL-4, and IL-6 are also available as GMP-grade proteins.

Learn more | rndsystems.com/proteins

B Cell-related ELISA Kits
R&D Systems offers complete Quantikine® ELISA Kits and DuoSet® ELISA
Development Systems for detecting B cell-secreted cytokines

Select B Cell-related ELISA Kits

Molecule Species Quantikine® ELISA Catalog # DuoSet® ELISA Catalog #
Human D6050 DY206
IL-6
Mouse M6000B DY406
Human D1000B DY217B
IL-10
Mouse M1000B DY417
Human DB100B DY240
TGF-b1
Mouse MB100B DY1679
Human DTA00C DY210
TNF-a
Mouse MTA00B DY410

B Cell ELISpot Development Modules

B Cell ELISpot Development Modules contain the basic components required to develop an
ELISpot assay to detect IgE, IgG, or IgM-secreting B cells.

B Cell ELISpot Development Modules

Kit Catalog #
Human IgE B Cell ELISpot Development SELB001
Human IgG B Cell ELISpot Development SELB002
Human IgM B Cell ELISpot Development SELB003
Mouse IgG B Cell ELISpot Development SELB004
Mouse IgM B Cell ELISpot Development SELB005

Tocri-2945
RnDSy-2945 Novus-2945

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