SAFETY DATA SHEET

Creation Date 10-Apr-2014 Revision Date 24-Dec-2021 Revision Number 5

1. Identification
Product Name Crystal Violet (Certified Biological Stain)

Cat No. : C581-25; C581-100

CAS No 548-62-9
Synonyms Gentian Violet; Basic Violet 3

Recommended Use Laboratory chemicals.

Uses advised against Food, drug, pesticide or biocidal product use.

Details of the supplier of the safety data sheet

Company
Fisher Scientific Company
One Reagent Lane
Fair Lawn, NJ 07410
Tel: (201) 796-7100

Emergency Telephone Number CHEMTRECÒ, Inside the USA: 800-424-9300

CHEMTRECÒ, Outside the USA: 001-703-527-3887

2. Hazard(s) identification
Classification
This chemical is considered hazardous by the 2012 OSHA Hazard Communication Standard (29 CFR 1910.1200)

Acute oral toxicity Category 4

Serious Eye Damage/Eye Irritation Category 1
Carcinogenicity Category 2

Label Elements

Signal Word
Danger

Hazard Statements
Harmful if swallowed
Causes serious eye damage
Suspected of causing cancer

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Precautionary Statements
Prevention
Obtain special instructions before use
Do not handle until all safety precautions have been read and understood
Use personal protective equipment as required
Wash face, hands and any exposed skin thoroughly after handling
Do not eat, drink or smoke when using this product
Response
IF exposed or concerned: Get medical attention/advice
Eyes
IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
Immediately call a POISON CENTER or doctor/physician
Ingestion
IF SWALLOWED: Call a POISON CENTER or doctor/physician if you feel unwell
Rinse mouth
Storage
Store locked up
Disposal
Dispose of contents/container to an approved waste disposal plant
Hazards not otherwise classified (HNOC)
Very toxic to aquatic life with long lasting effects
WARNING. Cancer - https://www.p65warnings.ca.gov/.

3. Composition/Information on Ingredients
Component CAS No Weight %
C.I. Basic violet 1 548-62-9 ~ 100.0

4. First-aid measures
General Advice If symptoms persist, call a physician.

Eye Contact Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Get
medical attention.

Skin Contact Wash off immediately with plenty of water for at least 15 minutes. If skin irritation persists,
call a physician.

Inhalation Remove to fresh air. If not breathing, give artificial respiration. Get medical attention if
symptoms occur.

Ingestion Clean mouth with water and drink afterwards plenty of water. Get medical attention if
symptoms occur.

Most important symptoms and None reasonably foreseeable. Causes severe eye damage.
effects
Notes to Physician Treat symptomatically

5. Fire-fighting measures

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Suitable Extinguishing Media Water spray, carbon dioxide (CO2), dry chemical, alcohol-resistant foam.

Unsuitable Extinguishing Media No information available

Flash Point Not applicable

Method - No information available

Autoignition Temperature No information available

Explosion Limits
Upper No data available
Lower No data available
Sensitivity to Mechanical Impact No information available
Sensitivity to Static Discharge No information available

Specific Hazards Arising from the Chemical

Thermal decomposition can lead to release of irritating gases and vapors. Do not allow run-off from fire-fighting to enter drains or
water courses.

Hazardous Combustion Products

Carbon monoxide (CO). Carbon dioxide (CO2). Nitrogen oxides (NOx). Hydrogen chloride gas.
Protective Equipment and Precautions for Firefighters
As in any fire, wear self-contained breathing apparatus pressure-demand, MSHA/NIOSH (approved or equivalent) and full
protective gear.

NFPA
Health Flammability Instability Physical hazards
2 1 1 N/A

6. Accidental release measures

Personal Precautions Use personal protective equipment as required. Ensure adequate ventilation. Avoid dust
formation.
Environmental Precautions Do not flush into surface water or sanitary sewer system. Do not allow material to
contaminate ground water system. Prevent product from entering drains. Local authorities
should be advised if significant spillages cannot be contained.

Methods for Containment and Clean Sweep up and shovel into suitable containers for disposal. Keep in suitable, closed
Up containers for disposal.

7. Handling and storage

Handling Wear personal protective equipment/face protection. Ensure adequate ventilation. Avoid
dust formation. Do not get in eyes, on skin, or on clothing. Avoid ingestion and inhalation.

Storage. Keep containers tightly closed in a dry, cool and well-ventilated place. Protect from direct
sunlight. Incompatible Materials. Strong oxidizing agents. Strong acids. Strong reducing
agents.

8. Exposure controls / personal protection

Exposure Guidelines This product does not contain any hazardous materials with occupational exposure
limitsestablished by the region specific regulatory bodies.

Engineering Measures Ensure adequate ventilation, especially in confined areas. Ensure that eyewash stations
and safety showers are close to the workstation location.

Personal Protective Equipment

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Eye/face Protection Wear appropriate protective eyeglasses or chemical safety goggles as described by
OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard
EN166.

Skin and body protection Wear appropriate protective gloves and clothing to prevent skin exposure.

Respiratory Protection Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard
EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if
exposure limits are exceeded or if irritation or other symptoms are experienced.

Hygiene Measures Handle in accordance with good industrial hygiene and safety practice.

9. Physical and chemical properties

Physical State Solid
Appearance Green
Odor Odorless
Odor Threshold No information available
pH No information available
Melting Point/Range No data available
Boiling Point/Range Not applicable
Flash Point Not applicable
Evaporation Rate Not applicable
Flammability (solid,gas) No information available
Flammability or explosive limits
Upper No data available
Lower No data available
Vapor Pressure negligible
Vapor Density Not applicable
Specific Gravity No information available
Solubility Soluble in water
Partition coefficient; n-octanol/water No data available
Autoignition Temperature No information available
Decomposition Temperature No information available
Viscosity Not applicable
Molecular Formula C25H30ClN3
Molecular Weight 407.6831

10. Stability and reactivity

Reactive Hazard None known, based on information available

Stability Stable under normal conditions. Light sensitive.

Conditions to Avoid Avoid dust formation. Incompatible products. Excess heat. Exposure to light.

Incompatible Materials Strong oxidizing agents, Strong acids, Strong reducing agents

Hazardous Decomposition Products Carbon monoxide (CO), Carbon dioxide (CO2), Nitrogen oxides (NOx), Hydrogen chloride
gas

Hazardous Polymerization Hazardous polymerization does not occur.

Hazardous Reactions None under normal processing.

11. Toxicological information

Acute Toxicity

Product Information
Component Information

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Component LD50 Oral LD50 Dermal LC50 Inhalation

C.I. Basic violet 1 LD50 = 420 mg/kg ( Rat ) Not listed Not listed

Toxicologically Synergistic No information available

Products
Delayed and immediate effects as well as chronic effects from short and long-term exposure

Irritation Severe eye irritant

Sensitization No information available

Carcinogenicity The table below indicates whether each agency has listed any ingredient as a carcinogen.

Component CAS No IARC NTP ACGIH OSHA Mexico

C.I. Basic violet 1 548-62-9 Group 2B Not listed Not listed X Not listed
Mutagenic Effects No information available

Reproductive Effects No information available.

Developmental Effects No information available.

Teratogenicity No information available.

STOT - single exposure None known

STOT - repeated exposure None known

Aspiration hazard No information available

Symptoms / effects,both acute and No information available

delayed

Endocrine Disruptor Information No information available

Other Adverse Effects The toxicological properties have not been fully investigated.

12. Ecological information

Ecotoxicity
Very toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment. The product contains following
substances which are hazardous for the environment.

Component Freshwater Algae Freshwater Fish Microtox Water Flea

C.I. Basic violet 1 EC50 = 0.025 - 0.8 mg/l, 72 Not listed Not listed EC50 = 0.24 - 5 mg/l, 48 h
h (Pseudokirchneriella (Daphnia magna (Water
subcapitata) flea)) OECD 202
OECD 201

Persistence and Degradability Soluble in water Persistence is unlikely based on information available.

Bioaccumulation/ Accumulation No information available.

Mobility . Will likely be mobile in the environment due to its water solubility.

Component log Pow

C.I. Basic violet 1 0.51

13. Disposal considerations

Waste Disposal Methods Chemical waste generators must determine whether a discarded chemical is classified as a
hazardous waste. Chemical waste generators must also consult local, regional, and
national hazardous waste regulations to ensure complete and accurate classification.

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14. Transport information

DOT
UN-No UN3077
Proper Shipping Name Environmentally hazardous substances, solid, n.o.s.
Technical Name C.I. Basic violet 1
Hazard Class 9
Packing Group III
TDG
UN-No UN3077
Proper Shipping Name Environmentally hazardous substances, solid, n.o.s.
Hazard Class 9
Packing Group III
IATA
UN-No UN3077
Proper Shipping Name Environmentally hazardous substances, solid, n.o.s.
Hazard Class 9
Packing Group III
IMDG/IMO
UN-No UN3077
Proper Shipping Name Environmentally hazardous substances, solid, n.o.s.
Hazard Class 9
Packing Group III
15. Regulatory information
United States of America Inventory

Component CAS No TSCA TSCA Inventory notification - TSCA - EPA Regulatory

Active-Inactive Flags
C.I. Basic violet 1 548-62-9 X ACTIVE -

Legend:
TSCA US EPA (TSCA) - Toxic Substances Control Act, (40 CFR Part 710)
X - Listed
'-' - Not Listed

TSCA 12(b) - Notices of Export Not applicable

International Inventories
Canada (DSL/NDSL), Europe (EINECS/ELINCS/NLP), Philippines (PICCS), Japan (ENCS), Japan (ISHL), Australia (AICS), China (IECSC), Korea
(KECL).

Component CAS No DSL NDSL EINECS PICCS ENCS ISHL AICS IECSC KECL
C.I. Basic violet 1 548-62-9 X - 208-953-6 X X X X X KE-07006

KECL - NIER number or KE number (http://ncis.nier.go.kr/en/main.do)

U.S. Federal Regulations

SARA 313 Not applicable

SARA 311/312 Hazard Categories See section 2 for more information

CWA (Clean Water Act) Not applicable

Clean Air Act Not applicable

OSHA - Occupational Safety and Not applicable

Health Administration

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CERCLA Not applicable

California Proposition 65 This product contains the following Proposition 65 chemicals.

Component CAS No California Prop. 65 Prop 65 NSRL Category

C.I. Basic violet 1 548-62-9 Carcinogen - Carcinogen
U.S. State Right-to-Know Not applicable
Regulations

U.S. Department of Transportation

Reportable Quantity (RQ): N
DOT Marine Pollutant N
DOT Severe Marine Pollutant N

U.S. Department of Homeland This product does not contain any DHS chemicals.
Security

Other International Regulations

Mexico - Grade No information available

Authorisation/Restrictions according to EU REACH

Component REACH (1907/2006) - Annex XIV - REACH (1907/2006) - Annex XVII - REACH Regulation (EC
Substances Subject to Restrictions on Certain Dangerous 1907/2006) article 59 - Candidate
Authorization Substances List of Substances of Very High
Concern (SVHC)
C.I. Basic violet 1 - Use restricted. See item 28. SVHC Candidate list - Carcinogenic
(see link for restriction details) (Article 57a)
Use restricted. See item 72.
(see link for restriction details)
Use restricted. See item 75.
(see link for restriction details)

After the sunset date the use of this substance requires either an authorization or can only be used for exempted uses, e.g. use in
scientific research and development which includes routine analytics or use as intermediate.

https://echa.europa.eu/authorisation-list
https://echa.europa.eu/substances-restricted-under-reach
https://echa.europa.eu/candidate-list-table

Safety, health and environmental regulations/legislation specific for the substance or mixture

Component CAS No OECD HPV Persistent Organic Ozone Depletion Restriction of

Pollutant Potential Hazardous
Substances (RoHS)
C.I. Basic violet 1 548-62-9 Not applicable Not applicable Not applicable Not applicable

Component CAS No Seveso III Directive Seveso III Directive Rotterdam Basel Convention
(2012/18/EC) - (2012/18/EC) - Convention (PIC) (Hazardous Waste)
Qualifying Quantities Qualifying Quantities
for Major Accident for Safety Report
Notification Requirements
C.I. Basic violet 1 548-62-9 Not applicable Not applicable Not applicable Not applicable

16. Other information

Prepared By Regulatory Affairs
Thermo Fisher Scientific
Email: EMSDS.RA@thermofisher.com

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Creation Date 10-Apr-2014

Revision Date 24-Dec-2021
Print Date 24-Dec-2021
Revision Summary This document has been updated to comply with the US OSHA HazCom 2012 Standard
replacing the current legislation under 29 CFR 1910.1200 to align with the Globally
Harmonized System of Classification and Labeling of Chemicals (GHS).

Disclaimer
The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the
date of its publication. The information given is designed only as a guidance for safe handling, use, processing, storage,
transportation, disposal and release and is not to be considered a warranty or quality specification. The information
relates only to the specific material designated and may not be valid for such material used in combination with any other
materials or in any process, unless specified in the text

End of SDS

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