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Lab#9 - Movement in Plants

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CSEC BIOLOGY LABS

Ms. Ramlogan
DATE:

TITLE: Transport in Plants

AIM: To observe the movement of water through a plant, Peperomia pellucida.

APPARATUS/ MATERIALS:

• Four small plants


• Water
• Beaker

METHOD:
1. The roots of the plants were washed to remove the soil.
2. Two plants were placed in a beaker containing water.
3. One was placed in a beaker containing blue dye and the other was placed in a beaker
containing orange dye.
4. The plants were left for several days.
5. The plants were then removed, and the stems were cut through for each plant.
6. The stems were compared and annotated diagrams were drawn.

RESULTS:
Draw a cross - section of the Peperomia pellucida and label it clearly to show the parts that
were covered by the dye.

DISCUSSION:

• Name the tissue involved in water transport and state one adaptation for this purpose.
• How does water enter the root of a plant?
• Explain the three ways water moves up the xylem- root pressure capillary action and
transpiration pull.
• What was noticed after the experiment and explain the observations? Explain how does
the coloured dye reached various parts of the plant.

PRECAUTIONS:

1. Ensure that there was no puncture or bruise on the stem since damaged xylem vessels
prevent capillarity action.
2. Ensure that the plants are placed in the same volume of solution.

SOURCES OF ERROR:

1. Some of the leaves were unhealthy therefore the stomata were closed.
2. Poor uptake of water by plants and therefore conditions do not encourage transpiration.
CSEC BIOLOGY LABS
Ms. Ramlogan
LIMITATIONS:

• Stems were thicker in some plants hence they have more xylem vessels.

REFLECTION: How can this experiment be improved? What is the relevance of this
experiment to you and society?

Suggested ways that experiment can be improved:


• Add a paper collar or oil around the stem to reduce evaporation of the coloured dye from
the flask.
• Measure the initial volume of dye solution at fixed intervals.
• Leave the stems for a longer time period and then observe.
• Using plants with a more transparent stem since this allows the movement of dye
upwards through the plant.
CONCLUSION: State any conclusions and relate them to the aim.

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