Chemistry

Chemistry project
Name: Rishi Shah

Class: 12th
Teacher: Rumita
Sengupta
School: SNBP
International school
Subject code:043
SNBP’s INTERNATIONAL SCHOOL,
MANJRI
CERTIFICATE
This is to certify that, Rishi shah a student of

th
class 12 has successfully completed the
project under
the guidance of Mrs. Ankita Singh during the year 2023-
2024 in the partial fulfilment of practical examination
conducted by AISSCE, New Delhi.
Date
School Stamp
Principal’s Signature
Signature of English Teacher

ACKNOWLEDGEMENT
I have taken efforts in this project. However, it would not have been
possible without the kind support and help of many individuals.
I would like to thank my principal

and school for providing me with facilities required to do my project.
I am highly indebted to my English teacher,

for her invaluable guidance which has
sustained my efforts in all the stages of this project work.
I would also like to thank my parents for their continuous support and
encouragement.
My thanks and apperceptions also go to my fellow classmates and

laboratory assistant in developing the project and to the people who
have willingly helped me out with their abilities.
INDEX
SR TOPIC
NO.
1. Certificate
2. Acknowledgement
3. Main content
4. Bibliography
Study of digestion of starch by salivary amylase and effect of

pH and temperature on it.
The main objective of this chemistry project

report is “To Study the digestion of starch by
salivary amylase and effect of temperature
and pH on it” and
1) To study digestion of starch by saliva.
2) To study the effect of temperature on the digestion of starch
by saliva.
3) To study the effect of pH on the salivary digestion of starch.
Introduction
Every Health book insists on the chewing of food. The act of
chewing stimulates the excretion of saliva. Saliva mixes up with
the food and helps its digestion. That is, the enzyme ptyalin or
amylase present in human saliva hydrolyzes the big molecules
of food into many molecules. For example starch into mono-
saccnaricies maltose and glucose; proteins into amino acids and
fats into fatty acids and glycerol. Thus saliva not only helps in
digestion of food but convert it into energy generating
substances. Further, enzymes and their activity are very
sensitive to temperature and ph. Even a slight variation in these
two factors, can disturb the action of enzymes. In other words,
digestion of food by salivary amylase is also effected by pH and
temperature and can be verified experimentally. For example,
hydrolysis of starch can be verified by testing it with iodine
solution. Starch forms blue coloured complex with iodine. If no
starch is present in a system it will not give blue colour with
iodine.
Material Required
The requirements for experiment of Chemistry Project
Report are as under:
1) Test tubes
2) Test tube stand
3) One dropper
4) Beaker
5) Stop watch
6) Starch and Iodine solution
7) Thermometer
8) Dilute HCL and Dilute NaOH solution.
Procedure for Chemistry Experiment 1 is:
1) Collection of Saliva – Rinse mouth throughly with cold

water and ensure that it does not contain any food particles.
Now take about 20ml of lupe warm water in the mouth and
gangle for about three minutes so that saliva mixes up well
with it. Spit this into a beaker. Filter, if there is any
suspended impurity clear filtrate is saliva solution and
contains enzyme ptyalin.
2) Preparation of starch solution – Take about 0.5g of starch
in 100ml beaker and add enough water to make a paste.
Dilute the paste by adding 50ml water and boil for about 5
minutes.
3) Digestion of starch :-
(a) Take 5ml of the starch solution in a test tube. Add
2ml of saliva solution into it. Mix the solutions well by
shaking the tube carefully and start a step watch.
(b) After one minute take out two drops of the mixture
solution from the test tube with the help of a dropper and
transfer it into another test tube containing about one 1ml
of 1% iodine solution. Note the colour produced, if any.
(c) Repeat this test after every one minute taking two
drops of the mixture solution and fresh 1% iodine
solution continue until the test shows no blue colour.
Record the time and blue colour intensity.
Observation
Time 1 2 3 4
Passed Minute Minute Minute Minute
after
Mixing
Colour Deep Blue Light No Blue
Intensity Blue Blue
Absence of blue colour on addition to iodine solution means

absence of starch in the mixture solution. That is whole of
the starch has got digested or hydrolysed.
Procedure: - Effect of temperature on the digestion of starch
by saliva.
1) Take three test tubes and label these 1, 2, and 3.

2) Take 5ml of the starch solution, 2ml of the saliva solution and
5ml of water in each test tube.
3) Place test tube Number 1 in water at room temperature, test
tube Number 2 in a beaker containing water at 500 C and test
tube Number 3 in boiling water.
4) After 5 minutes, observe the colour change on mixing two
drops of the mixture of every tube with one ml of 1% iodine
solution. Note the intensity of blue coloured form.
Conclusion
Starch get hydrolysed by saliva amylase
Chemistry Experiment 3
Procedure: - To study the effect of pH on the salivary

digestion of starch
1) Take three test tubes and label these 1, 2, and 3.

2) Add 5ml of the starch solution, 2ml of the saliva solution in
each test tube.
3) Now ass 2ml of water in test tube Number 1 2ml of Dilute
HCL in test tube Number 2 and 2ml of Dilute NaOH solution
in test tube Number 3 and shake carefully.
4) Keep the three test tubes in water at room temperature for
about 10 minutes.
5) Add 2 drops of the solution of each test tube with 1% iodine
solution and observe the colour change
Conclusion
Temperature effects the digestion of starch by salivary with

increase in temperature salivary amylase get inactivated and
process of digestion do not take place.
BIBLIOGRAPHY
1 http://www.google.com/
2 http://en.wikipedia.org
3 N.C.E.R.T Class 12 Chemistry
4 Dinesh Chemistry
5 Comprehensive Chemistry

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Chemistry project

Name: Rishi Shah

This is to certify that, Rishi shah a student of

Signature of English Teacher

I would like to thank my principal

I am highly indebted to my English teacher,

My thanks and apperceptions also go to my fellow classmates and

Study of digestion of starch by salivary amylase and effect of

The main objective of this chemistry project

2) Test tube stand

6) Starch and Iodine solution

8) Dilute HCL and Dilute NaOH solution.

Procedure for Chemistry Experiment 1 is:

1) Collection of Saliva – Rinse mouth throughly with cold

Absence of blue colour on addition to iodine solution means

1) Take three test tubes and label these 1, 2, and 3.

Starch get hydrolysed by saliva amylase

Procedure: - To study the effect of pH on the salivary

1) Take three test tubes and label these 1, 2, and 3.

Temperature effects the digestion of starch by salivary with

3 N.C.E.R.T Class 12 Chemistry

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