Science Reviewer.

Module 1:
Photosynthesis is the food-making process which converts light energy from

the sun into chemical energy in food. The process requires molecules of water
and carbon dioxide in the presence of sunlight and chlorophyll to produce the
food (glucose) and release the by-product oxygen. Organisms which contain
chlorophyll like algae and plants can perform photosynthesis. The
chlorophylls of the chloroplast are the green pigment which absorbs the light
energy from the sun. Inside the chloroplasts are disc shaped structures called
thylakoids which contain chlorophylls. Thylakoids are stacked to form a
granum. Not all organisms which can perform photosynthesis possess
chloroplasts. If chlorophylls are present in any part of the organism, food
making can take place.
The leaves are the main area for photosynthesis. The leaf has mesophyll
layers in between the upper and lower epidermis. Each epidermis has stomata
which allows the carbon dioxide to enter and the oxygen to leave. Stomates
are guard cells which regulate the gas exchange in the leaves. When the guard
cells absorb water, it becomes turgid and opens. When it loses water, it
becomes flaccid and closes. Lower epidermis has more stomates than upper
epidermis. The mesophyll layers are the palisade and the spongy.
Photosynthesis occurs more on the palisade with closely packed parenchyma
cells. The spongy layer is loosely arranged to allow gas to move.
Photosynthesis is divided into two phases. The light dependent phase and the
light independent phase. The light dependent phase occurs in the thylakoids
of the chloroplast. It requires the presence of light energy and water. This part
produces ATP and NADPH needed for the next phase of photosynthesis
The absorption of light in the light dependent phase or light reaction involves
two photosystems: PI and PII. The photosystems contain light harvesting
pigments and proteins. The light harvesting pigments passes the energy to
the reaction center. PII contains the reaction center P680 and the PI contains
the reaction center P700.
When the light energy strikes the leaves, chlorophylls in photosystem II
capture the energy and passes it to the P680 reaction center. The energy
excites the electrons of the chlorophyll and moves to a series of electron
acceptors. The lost electron is replaced by the electron from the water due to
photolysis (the splitting of water into H ion, electron, and oxygen due to the
heat from the sun)
The same event occurs in photosystem II. The high energy electron ejected
out and moves to a series of electron acceptors. In the PII, the lost electrons
are replaced by electrons from water while in PI, the lost electrons are
replaced by the electrons from PII. The final electron acceptor in the process
is NADP- to produce NADPH. It is very important to remember that the
movement of energy in both photosystems is simultaneous since electrons in
both P680 and P700 are activated by the energy received from the sun.
As the high energy electron moves from one acceptor to another, it loses
energy. This released energy is used to produce ATP in the presence of the
enzyme
ATP synthase. The production of ATP can be of two ways. Cyclic
Photophosphorylation and Non-cyclic Photophosphorylation. In the non-cyclic
photophosphorylation, the electron moves from the PII. to PI, until the last
electron acceptor NADP-. In this process ATP and NADPH are produced. In
the cyclic photophosphorylation, the electrons follow a cyclic path in
producing ATP. It is important to remember that in light-dependent reaction,
the following
events occurred: 1. activation of the electron in the chlorophylls of P680 and
P700; 2. photolysis; and 3. Photophosphorylation: Non-cyclic and Cyclic. The
raw material is water, and the products are ATP and NADPH. Oxygen is a by-
product. The light-independent phase of photosynthesis occurs in the stroma
of the chloroplasts. It is also called Calvin Cycle or Dark reaction. This process
occurs once the products of the light-dependent reaction are available.
The Calvin cycle has four main steps: carbon fixation, reduction phase,
carbohydrate formation, and regeneration phase. Energy to fuel chemical
reactions in this sugar-generating process is provided by ATP and NADPH,
chemical compounds which contain the energy plants have captured from
sunlight.
Carbon fixation. Carbon in the molecule of carbon dioxide (CO2) is fixed from
an inorganic to an organic molecule. An enzyme Rubisco combines carbon
dioxide and RuBp to produce an organic molecule with six carbon organic
compound.
Reduction Phase. the six-carbon compound is reduced into phosphoglyceric
acid (3-PGA) and further converted into phosphoglyceraldehyde (G3P) using
electrons supplied by ATP and NADPH, then ATP and NADPH are converted to
ADP and NADP+, respectively.
Carbohydrate Formation. The G3P will be converted into glucose. Two G3Ps
are needed to make one glucose. For every turn of the cycle, only one carbon
dioxide molecule is needed to produce one G3P, thus six carbon dioxide
molecules are needed to make two G3P for one glucose molecule and the rest
for regeneration.
Regeneration Phase. The G3Ps produced in the cycle are used to make
glucose and regenerate RuBP. Three more molecules of ATP are used in these
regeneration reactions.
It is important to remember that the Calvin Cycle uses six carbon dioxides to
produce one glucose. In the process, ATPs and NADPH from the light reaction
are used to fix the carbon dioxide and produce glucose and regenerate the
RuBP. It is called light independent phase because it is not directly powered
by light.

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Module 1:

Photosynthesis is the food-making process which converts light energy from

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