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    Lab Report 2

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    sara.a.alabdulkarim
    This lab report describes bacterial culture techniques including transferring bacteria between media using aseptic techniques and isolating single bacterial species using plate streaking. The objective was to learn how to subculture bacteria between broth, agar slants, and agar plates while maintaining aseptic technique. The procedure involved streaking bacteria in a quadrant pattern on agar plates from broth cultures to isolate single colonies, and transferring bacteria between broth and agar media using flaming loops. Results showed bacterial growth on agar plates as isolated colonies and along the streaked line on agar slants.

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    Lab Report 2

    Uploaded by

    sara.a.alabdulkarim
    18 views4 pages
    This lab report describes bacterial culture techniques including transferring bacteria between media using aseptic techniques and isolating single bacterial species using plate streaking. The objective was to learn how to subculture bacteria between broth, agar slants, and agar plates while maintaining aseptic technique. The procedure involved streaking bacteria in a quadrant pattern on agar plates from broth cultures to isolate single colonies, and transferring bacteria between broth and agar media using flaming loops. Results showed bacterial growth on agar plates as isolated colonies and along the streaked line on agar slants.

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    This lab report describes bacterial culture techniques including transferring bacteria between media using aseptic techniques and isolating single bacterial species using plate streaking. The objective was to learn how to subculture bacteria between broth, agar slants, and agar plates while maintaining aseptic technique. The procedure involved streaking bacteria in a quadrant pattern on agar plates from broth cultures to isolate single colonies, and transferring bacteria between broth and agar media using flaming loops. Results showed bacterial growth on agar plates as isolated colonies and along the streaked line on agar slants.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    18 views4 pages

    Lab Report 2

    Uploaded by

    sara.a.alabdulkarim
    This lab report describes bacterial culture techniques including transferring bacteria between media using aseptic techniques and isolating single bacterial species using plate streaking. The objective was to learn how to subculture bacteria between broth, agar slants, and agar plates while maintaining aseptic technique. The procedure involved streaking bacteria in a quadrant pattern on agar plates from broth cultures to isolate single colonies, and transferring bacteria between broth and agar media using flaming loops. Results showed bacterial growth on agar plates as isolated colonies and along the streaked line on agar slants.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    Lab Report 2

    Bacterial Culture Techniques


    - BIO 223 L -

    Student Name: Sara Alabdulkarim


    Student ID: 201136
    Submission date: September 26, 2021

    Abstract:

    Objective:

    To transfer bacteria between different types of media cultures in an aseptic manner


    and to learn how to isolate a single species in pure culture by plate streaking
    technique.

    Introduction:

    Materials:
     Nutrient broth and nutrient agar slant culture.
     Nutrient agar plates.
     Agar slant, broth tubes.
     Disposable inoculating loops.
     Flame.
    Procedure:
    (A) Aseptic Transfer Techniques:

    I. From Broth to Agar slant:

    1. Flame the mouth of the broth tube before you start.


    2. Insert the loop into the broth culture and withdraw a loopful of liquid. You
    should see watery film on the loop and re-flame the mouth of the tube.
    3. The broth-containing loop is immediately inserted into the end of the agar
    slant tube.
    4. Place the loop flat on the agar surface and move the loop rapidly in a zigzag
    motion, gently across the agar surface from bottom to top.
    5. Remove and dispose the loop.

    II. From Agar plate to Broth:

    1. Flame the mouth of the broth tube before you start.


    2. Insert the loop into the agar plate culture and touch the agar surface where
    the bacteria are growing.
    3. Remove a minute sample of bacteria.
    4. Insert the loop is immediately into the broth tube.
    5. Shack the loop gently to suspend the bacteria.
    6. Withdraw the loop from the broth and dispose it.

    (B) Quadrant Streak plate Technique:

    1. Select an agar plate and label the bottom side of the plate by group number
    and the date.
    2. Obtain a broth culture and Flame the mouth of the broth tube before you
    start.
    3. Insert the loop into the broth culture and withdraw a loopful of liquid and re-
    flame the mouth of the tube.
    4. Streak the loop (zigzag motion) along one area/quadrant of the plate.
    5. Pick a new loop and streak some of the bacteria from the first area to the
    second area.
    6. Repeat step 5 on the third and fourth areas. Replace the lid.
    Invert the plate and incubate it for 24 h at the appropriate temperature
    Results:

    Figure 1: Agar plate with growing bacteria . Figure 2: Agar slant with bacterial cultures.

    Figure 3: Agar broth, slight bacterial growth.


    Discussion:
    In this lab we preformed Aseptic Transfer of bacteria between different types of
    media culture. First one as an agar plate where we took a loopful of specimen and
    transferred it on to the surface or the plate by streaking a series of parallel lines in
    different parts of the plate that resulted in separated colonies over the streaked
    segments. Second one was the broth where we picked up a segment of the bacteria
    and twirled it around in the broth and results were that the broth got “foggy”. As for
    the third and last one (agar slant) we again to a segment of the bacteria and dragged
    the loop in a ridged line across the slant, result where that the bacteria appeared on
    the surface across the slant ridged line we drew.

    References:
    o Lab manual.
    o Lab PowerPoint.
    o

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