Spinach Ink - Research Proposal - Revise
Spinach Ink - Research Proposal - Revise
Spinach Ink - Research Proposal - Revise
Region VIII
EXPERIMENTAL STUDY
ENTITLED
INK
Researchers:
Charity Maraon
Shary Labastida
Hazel S. Casono
Vhalyrie Del Valle
Axcel Tomaub
Chrisjoy Rodes
John Lloyd Tortor
Research Adviser:
0ctober, 2022
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Table of Contents
Page
Front page…………………………………………………………………………………....1
Table of Contents……………………………………………………………………………2
Methodology
Research Design…………………………………………………………………...…14
Materials………………………………………………………………..…………….14
General Procedure……………………………………………………………………14
Collection and Preparation………………………………………….…………….….15
Experimental Design…………………………………………………………………15
Statistical Treatment……………………………………………………………….…16
References…………………………………………………………………………………...18
Curriculum Vitae …………………………………………………………………………..20
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BACKGROUND OF THE STUDY
Rationale
For many years, natural materials including berries, bark, and leaf extracts
have been used to make ink. Ink is a liquid or paste containing dyes and pigments, used for
writing or drawing with a pen, brush, or quill or for marking a text, design, image, or colored
been used as raw ingredients to create various colors and to produce ink, dye, or paint when
mixed with other substances. Due to their ability to produce yellow, green, brown, or black
ink, tea leaves, have been used to make ink from ancient times, according to historical
documents. Numerous applications of ink, such as inkjet printing and bio-ink, have been
solvents, which are damaging to both people and the environment, are frequently used to
make inks. Unprotected contact with ink has the potential to cause serious headaches, skin
rashes, or harm to the neurological system, most likely as a result of the solvents or pigments,
like p-anisidine, that are present in the ink. This is the sole reason why consumers prefer
natural pigments over synthetic dyes. (Downham and Collins 2000). The harmful effect of
synthetically made ingredients of stamp pad inks drive the researchers to conduct a study of
using spinach (alugbati) fruit as the colorant of ink to lessen the use of the harmful substance.
In other research, only the color and the odor acceptability of alugbati-fruit-
based-ink are being studied. There are other significant elements that need to be looked out
for in making a better stamp pad ink like how fast it dries and how it absorbs (behavioral). In
this research, however, the study of absorption behavior, how fast it dries, and the difference
in its market price to the leading stamp pad ink product is included while the odor is excluded
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Objectives of the Study
This research study aims to know how effective to use spinach seed as a natural
colorant of ink for stamping pads. Researchers will study its quality in terms of:
1. Color it produces
2. Absorption
4. Longevity
The finished product will be compared to its market price to the leading stamp pad
brand.
The study aims to determine the effectiveness of the alugbati fruit extract as an
alternative ink for stamp pad and hope to benefit the following:
To users. By using natural inks such as alugbati fruit extract, users can benefit
The health of the people. Conscious people are one of those who can benefit
from this product in terms of their health. Organic ink reduces the risks of exposure to
chemicals from commercial inks. Furthermore, the alugbati fruit extract ink has lower
toxicity than commercial inks in terms of odor and other factors, especially for young
children.
atmosphere and the surroundings from getting destroyed. Commercial inks for markers
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Scope and Limitations
The general intent of this study is to test the effectiveness of spinach seed as the
alternative dye for stamp pad ink. Like industrial-made stamping pads, there are additional
ingredients to enhance the capability of spinach fruit extract. Researchers will then
investigate its effectiveness in terms of color, its behavior during absorption, cost, and
especially the time it takes to dry. Also, this study is conducted with a limited amount of
financial resources and time framework. Hence, the process of making stamp pad ink is
mostly done manually; no machinery will be use, only tools. This study no longer includes
other possible parameters (i.e.texture of the paper). This research only investigates the extract
of the alugbati fruits, not the alugbati plant itself or its stem, leaves, and other parts. The
experimentation will be conducted on the premises of Sogod National High School (SNHS),
specifically the science laboratory. For the research equipment during the experimentation,
the researchers will be given the lab apparatus from the science laboratory. Researchers aim
Conceptual Framework
This study is about using alugbati fruit extract as an alternative ink for a stamp
pad. The independent variable is the alugbati fruit extract. Researchers are going to use it as a
substitute for inks in a stamp pad. The alugbati fruit extract is tested in order for the
dependent variables’ results to be shown. The dependent variables are color, absorption,
longevity, and the time it takes to dry. These are the variables that are to be observed.
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Input PROCESS
Phase I: Collecting Spinach
Mature Spinach seeds, ingredients, and
Fruit materials needed.
Glycerol
Reverse Osmosis/ Phase II: Preparation of
Demineralized Spinach Fruit Extract
Water Put the spinach seeds in an
Fire soot electric blender.
Electrical Blender Squeeze the Spinach puree
Steering Rod using a clean cloth to separate
Beaker the extract from its pulp.
Clean Cloth Put it in a pan and boil it in
Emptied Container half an hour.
Stamp Set aside.
Pan
Phase III: Making the ink
Measure the desired amount of
the spinach fruit extract along
with the other ingredients.
Prepare the Beaker.
Add the glycerin to the empty OUTPUT
beaker.
Stamp Pad Ink
Next. Add DM water to the
beaker.
Stir the solution with the
stirring rod.
Add the spinach fruit extract
and fire soot.
Stir the solution again and mix
it well.
After mixing, let it rest for
about an hour.
Put the finished product in an
empty container.
Feedback
I. Testing of the ink in terms of the quality and effectiveness of the following:
1.1 Stick Consistency
1.2 Color it produces
1.3 Absorption
1.4 Time it takes to dry
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Figure 1
Ink is invented four thousand five hundred years ago. It was invented by both
Egyptians and Chinese around the same time. It has components of mainly two: pigment and
the carrier. The pigment is the dye itself, this is what the carrier transfers to the paper or
printing medium. Ink is usually colored, however, the early ink utilized fire soot or charcoal
as the primary pigment, which is why the majority of the earliest written works were created
in black ink. Additionally, charcoal was a pigment that was inexpensive and widely available,
The pigment is blended with the carrier (or vessel) in order to transmit it to the
media. Despite the fact that this solution must be a liquid of some kind, oil-based containers
are superior at securing the pigment to the medium. Currently, this is typically some kind of
vegetable oil, whereas, in the early days of ink production, the vessel was frequently some
drying properties of petroleum-based inks enabled newspapers to be printed and dried much
faster than with non-petroleum ink carriers in the early 1900s, resulting in the popularity of
these chemical ink combinations. With the advent of the 1970s, as oil prices skyrocketed
during the oil crisis, printers began looking for alternatives. As a result, vegetable-based inks
and other eco-friendly inks have gained popularity. These inks are far more environmentally
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The printing revolution really took off with the development of the Gutenberg
printing press in 1457 (based on much older Chinese discoveries like block printing and
moveable type). Although only available to a select few, printing presses employed an oil-
based ink and enabled mass communication. Businesses were able to create their own modes
of communication faster once the typewriter was created in 1860 and put into mass
production in 1870. Typewriters eventually gave way to printers, of which daisy wheel and
dot matrix printers were the earliest varieties. Inkjet printers were created in the 1970s and
were made accessible to homes in the late 1980s. Inkjet printers employ dye inks with a water
base. wherein tiny particles are sprayed onto paper. Since the dye's solvent is absorbed by the
paper, the color is guaranteed to stay. However, toner—a powdered form of ink—is
transferred to paper by laser printers via an electrostatic method. Both contemporary printers
Modern ink formulations are far more complicated. In addition to the pigment,
premature drying, polymeric resins to impart binding, and other properties. Thickeners or
prevent bacterial and fungal growth that causes fouling, and wetting agents like surfactants to
control surface properties. Over 90% of inks are printing inks, which use pigments rather than
the dyes found in writing inks to add color. Despite the fact that both phrases are occasionally
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used interchangeably in commercial literature, dyes are soluble whereas pigments are
insoluble. Both inorganic and organic colors are used in ink. The majority of red inks for
writing are diluted solutions of red dye ink. Triphenylmethane dyes that have been replaced
can provide blue color. In addition to dyes, many permanent writing inks also contain iron
sulfate, Gallic, and tannic acids. Ballpoint ink is typically a paste that contains 40–50% dye.
Stamp Pad its Ink: Definition, History, Application, and Its Significance
A stamp pad is a porous pad, often made of a felt-like material, impregnated with ink,
The history of stamps goes back more than 9,000 years. From cylinder seals to the
advanced digital stamps that are currently marketed online, it changed over the years.
Cylinder seals (7,800 BCE) – The cylinder seal was the first type of stamp. These
elaborate impression stamps were used to sign messages and seal business deals throughout
Mesopotamia. Each seal would have a completely distinct design. The cylinder was worn
around the owner's neck on a string, and whenever they needed to sign something, they
would take it off and roll it in wet clay to make an impression that acted something like a
signature.
Woodblock printing (220 CE) --The earliest example of what we would recognize as
stamping comes from ancient China and existed long before the invention of the printing
press. Called woodblock printing, the process involved carving images and characters into a
block of wood and then applying ink and fabric to form an impression. The process was used
to make decorative silks and books, and the oldest surviving example is the Diamond Sutra, a
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Wax seals (500 CE) – Like many seals, the wax seal first appeared in the Middle
Ages and was used to seal letters as well as the ends of official documents. Wax sealing was
adopted by the ruling classes, and as it spread, individuals in authority began to wear signet
rings, which are rings that bear the owner's seal, rather than carrying around handheld stamps.
This made it much simpler to transport and contributed to the development of traditions like
ring kissing.
The printing press (1450 CE) - Woodblock printing was commonplace in 14th-
century Europe but was costly and time-consuming. This led many metalsmiths to
experiment with movable type. Johannes Gutenberg perfected the technique in 1450,
adapting an old wine press and metal handset blocks. His invention became famous and made
it easier to mass-produce books like the Bible. Unfortunately, he didn’t get to enjoy his
invention for long, as it was taken from him in a lawsuit and given to one of his debtors.
Quick-drying ink (the 1800s) - Ink has been made from different substances over the
centuries, from soot and turpentine to lead and egg whites. Printers added chemicals to their
inks for a number of reasons. But it wasn’t until the 19th century that solvents were added,
Rubber stamps (the 1860s) - The rubber stamp came about in the mid-1800s after
Charles Goodyear discovered the vulcanization process. Soon after, rubber stamps became
commercially viable and were sold primarily to businesses for dating and pricing. In fact, one
of the oldest existing self-inking stamps is a vintage pricing stamp circa 1886. Inks for use
with rubber stamps are generally made by dissolving dyes in a mixture of glycerol and water.
They will not dry on the pad, because glycerol is practically non-volatile at ordinary
temperatures. The ink dries by being absorbed when an imprint is created on paper. If the
paper is absorbent, the ink may penetrate rapidly enough, but on well-sized paper, it may take
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many seconds, or even a few minutes, for the ink to be absorbed. If many papers must be
stamped in quick succession, the danger is incurred of blurring the impressions. Alcohol is
one of a number of volatile organic liquids that can be added to ink made with glycerol and
water, to make it sink rapidly into paper. Alcohol has the drawback of being much more
volatile than water, and if ink containing it is put on a stamp pad, much of the alcohol may be
Electronic stamps (modern-day) --Today, we sell a wide variety of stamps. Our most
futuristic is the Colop e-mark, which allows you to create, edit and transfer full-color
imprints. Our range of products can be customized to suit your needs and includes stamps of
A self-inking stamp can have the ability to mark thousands of impressions. For
marking impressions, you need to simply press it. This stamp can make a clear print on wood,
paper, or plastic. Self-inking stamps are manufactured from durable materials, allowing
frequent use on any kind of surface. As they are quite strong in nature, so you can use them
for so many purposes. Usually, they showcase a perfect blend of technology and quality;
which makes them one of the premier choices in the stamping industry. They do not need any
messy re-inking as the ink is already in the rubber. Just you need to remove the older pad and
replace it with a new one, without spilling it over your hands and clothes. They can last a
longer period; which makes them the best option to choose from for big business houses. You
can imprint your address, name logos, signatures, and much more with these simple stamping
accessories. They are available in the market at reasonable price quotes and rest assured that
their prices will never exceed your budget. You can find a wide array of self-inking stamps
with different shapes like round, square, or rectangle to suit your unique requirements. If you
think that they are only limited to black color, then you might be mistaken as there is a huge
assortment of color options; which you can choose as per your preferences and needs. These
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stamps can save a lot of time and effort for you as they just need you to press to make an
The National Bureau of Standards found out that Butyl Carbitol, the
monobutyl ether of diethylene glycol, is not only as efficient as alcohol for making glycerol-
water ink sink rapidly into paper, but is also so slowly volatile that a pad can be exposed to
the air for weeks, yet the ink will still be absorbed quickly.
Alugbati
Originally from India, alugbati (Basella alba L., B. rubra L.), also known as
Malabar spinach or Ceylon spinach, is commonly found in populated areas, hedges, former
agricultural land, etc. across the Philippines. It is frequently grown in market gardens and
backyard plots, and it is also offered for sale in supermarkets in the Visayas and Mindanao.
Additionally, it is grown in Malaysia, Africa, and tropical Asia. The plant is a silky, twining,
an herbaceous vine that is succulent. Either green or purple stems are present. The leaves are
5–12 centimeters (cm) long, fleshy, oval, or heart-shaped, stalked, tapering to the pointy tip,
and have a heart-shaped base. The axillary, solitary, 5-29 cm long spikes are present. The
pink blossoms measure approximately 4 mm in length. When ripe, the fruit is juicy, stalkless,
almost spherical in shape, 5-6 mm long, and purple. Alugbati comes in three popular
varieties: Basella alba, with a green stem and practically round, oval-shaped leaves; Basella
rubra, with a red stem and green, similar-shaped leaves; and a third variety, which is a cross
between the two. The plant has a lot of soluble fiber, which promotes healthy digestion. It
contains a lot of saponins, which function as phytochemicals and prevent cancer. The
antioxidant xanthones is abundant in the red species of alugbati. One of the B vitamins that is
essential for females who are pregnant or breastfeeding is folic acid, often known as folate. It
aids in preventing neural tube abnormalities in fetuses, for instance, spina bifida
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In the study conducted by the NATIONAL BUREAU OF STANDARDS, it
has been found that Butyl Carbitol, the monobutyl ether of diethylene glycol, is not only as
efficient as alcohol for making glycerol-water ink sink rapidly into paper, but is also so
slowly volatile that a pad can be exposed to the air for weeks, yet the ink will still be
absorbed quickly.
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Methodology
Research Design
This section contains information about the study's methodology, such as plans for
various stages, experiments, tools, specific procedures, or approaches. This study uses an
experimental design and uses spinach seed extract as a natural colorant dye ink (stamping
pad).
Materials
The main ingredient in the production of stamp pads is spinach (Spinacia Oleracea).
Reverse Osmosis/ Demineralized water and Glycerin were also used in the study. A
preliminary experiment was carried out in order to improve the formulation of the stamp pad.
A feasible formulation was used for the study, with all variables not held constant except the
level of pure Spinach (Spinacia Oleracea), which serves as the independent variable.
Demineralized Water is purified water that has all of its minerals removed through a process
called ion exchange. Raw water has many impurities, like magnesium, calcium, and sodium.
That is why, DM water is ideal to be an ingredient of stamp pad ink as the researchers do not
want any other chemicals to agitate the formulation as it may contaminate other substances
and interfere with the intended result. The Glycerin prevents the ink from drying out on the
sponge and prevents the ink to spread as it absorbs into the paper.
General Procedure
First, prepare and accumulate the ingredients and tools needed. Second,
prepare the spinach fruit juice/extract. And finally, make the stamp pad ink (see fig. I).
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Collection and Preparation
The spinach seeds were gathered in the locality of Sogod, Southern Leyte. The seeds
were thoroughly washed to remove the dust and other materials attached. After that, the
spinach seeds were turned into a paste-like form by the use of an electric blender. All
materials were then prepared for the utilization of the stamp pad. The complete process:
figure 2
Experimental Design
with one independent variable: the mixture of Spinach Seeds {Spinacia oleracea} and
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Demineralized water and glycerin. The study presented in Table 3 used a total of three
experimental replication for each treatment, including the stamp pad ink using organic
ingredients.
Distribution of Components
T1 20 55 25 5
T2 15 50 35 5
T3 20 50 30 5
Statistical Treatment
Quality testing will be used in the testing of stamp pad ink to notice the level of
Time dries – the extracted seeds were put on a clean paper using a drop of a 5ml syringe
while timed with minute check-ups until the seeds is fully dried.
Test 1.1
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Determine the time duration of the product when applied to minutes (Test 1 – Test 3)
Absorption – By using the first test researcher will check the time in the second to
Determine the time duration of the product when applied to seconds (Test 1 – Test 3)
Color produced – To determine what color it produced, the researcher will extract the juice
Longevity – the researcher will also test whether the Spinach seed extract will be much
more effective when it comes to the longevity of the product. To determine the longevity of
the product, researchers conduct a new test while timing with minute check-ups until the
Test 1.2
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References
extract-as-an-alternative-ink-for-markers2docx-pdf-free.html
https://www.scienceinschool.org/article/2015/artistic-introduction-anthocyanin-inks/
charcoal. (n.d.). In The Merriam-Webster.com Dictionary. Retrieved October 26, 2022, from
https://www.merriam-webster.com/dictionary/charcoal
https://www.scribd.com/document/444552672/Experimental-Research-2018
Final paper formulation of organic ink using ipomoea batatas. (n.d.). Retrieved October 26,
of-organic-ink-using-ipomoea-batatas
Johnson, S. (2019, March 2). What Is the Chemical Composition of Pen Ink? Sciencing.
https://sciencing.com/chemical-composition-pen-ink-17194.html
Pauser, D. (2019, January 10). How to Make Ink From Alugbati. Our Pastimes.
https://ourpastimes.com/make-ink-alugbati-6524991.html
Picincu, A. (2018, December 6). What Are the Benefits of Alugbati? Healthy Eating | SF
Gate. https://healthyeating.sfgate.com/benefits-alugbati-9863.html
Promotions, L. (2017a, October 2). Self Inking Stamp — Know How It Works - Laserwrite
how-it-works-e2b803d7bcf2
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Promotions, L. (2017b, October 21). Top 10 Benefits Of Using Self Inking Stamps In Sydney.
Medium. https://medium.com/@laserwritepro/top-10-benefits-of-using-self-inking-
stamps-in-sydney-89b13505d9ed
Tech Nexus 4U. (2017, December 28). Stamp Pad Ink Making 100% Real Formula [Video].
YouTube. https://www.youtube.com/watch?v=GomC60Gk5Mk
The World’s Fastest Dictionary | Vocabulary.com. (n.d.). Retrieved October 26, 2022, from
https://www.vocabulary.com/dictionary/stamp+pad?
fbclid=IwAR1InvOfRoKaYO3pVfFGcfyd_mr-
uDMzG4qMgCZ07NTtOjJ6lBFcQrnDxb4
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CURRICULUM VITAE
I. PERSONAL INFORMATION
Secondary:
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I. PERSONAL INFORMATION
Name: Chris Joy Rodes
Address: Ezperanza, Bontoc, Southern Leyte
Age: 17
Place of Birth: Ezperanza, Bontoc, Southern Leyte
Birthday: November 28, 2004
Civil Status: Single
Nationality: Filipino
Sex: Male
Religion: Roman Catholic
Parents / Guardian: Mr. and Mrs. Crispin and Abundia Rodes
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
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I. PERSONAL INFORMATION
Name: Vhalyrie Del Valle
Address: Hilaan, Bontoc, Southern Leyte
Age: 18
Place of Birth: Hilaan, Bontoc, Southern Leyte
Birthday: December 13, 2003
Civil Status: Single
Nationality; Filipino
Sex: Female
Religion: Iglesia Filipina Independiente (IFI)
Parents / Guardian: Edgardo Celeste
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
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I. PERSONAL INFORMATION
Name: John Lloyd Tortor
Address: San Vicente, Bontoc, Southern Leyte
Age: 18
Place of Birth: Bucana, Davao City
Birthday: September 23, 2004
Civil Status: Single
Nationality: Filipino
Sex: Male
Religion: Seventh Day Adventist
Parents / Guardian: Mrs. Loida and Mr. Bobby Tortor
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
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I. PERSONAL INFORMATION
Name: Axcel Bautista Tomaub
Address: Mauylab, Bontoc, Southern Leyte
Age: 17
Place of Birth: Mangaldan, Pangasinan
Birthday: May 28, 2005
Civil Status: Single
Nationality: Filipino
Sex: Male
Religion: Roman Catholic
Parents / Guardian: Mr. and Mrs. Jethro and Analyn Tomaub
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
24
I. PERSONAL INFORMATION
Name: Shary A. Labastida
Address: San Vicente, Bontoc, Southern Leyte
Age: 17
Place of Birth: San Vicente, Bontoc, Southern Leyte
Birthday: December 18, 2004
Civil Status: Single
Nationality; Filipino
Sex: Female
Religion: Seventh Day Adventist
Parents / Guardian: Mrs. Charon and Mr. Benjamin Labastida
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
25
I. PERSONAL INFORMATION
Name: Charity Maraon
Address: Divisoria, Bontoc, Southern Leyte
Age: 17
Place of Birth: Divisoria, Bontoc, Southern Leyte
Birthday: August 31, 2005
Civil Status: Single
Nationality: Filipino
Sex: Female
Religion: Roman Catholic
Parents / Guardian: Mr. and Mrs. Dante and Mary Grace Maraon
II. EDUCATIONAL BACKGROUND
Secondary:
(Senior High): Sogod National High School
Zone 1, Sogod Southern Leyte
Science Technology Engineering Mathematics (STEM)
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