Expt. No.

:
Date: 15/03/a014
Experiment Water Quality Monitoring: Total Dissolved Oxygen Assessment in
Different Water Samples by Winkler's Method
Problem definition Dissolved oxygen (DO) is essential to
living organisms in water but
harmful if present in boiler feed water
leading to boiler corrosion.
Methodology Winkler's titration method is used to assess DO in water.
Solution Estimation of total dissolved oxygen in different water
samples.
Student learning Students will learn to
outcomes a) perform Winkler's titration method
b) assessthe total dissolved oxygen in different water samples
Principle: Estimation of dissolved oxygen (DO) in water is useful in studying corrosion

effect of boiler feed water and in studying water pollution. DO is usually determined by
Winkler's titration method. t is based on the fact that DO oxidize potassium iodide (K]) to

iodine. The liberated iodine is titrated against standard sodium thiosulphate solution using

starch indicator. Since DO in water is in molecular state, as such it cannot oxidize KI. Hence,
and
manganese hydroxide is used as an oxygen carrier to bring about the reaction between KI
Oxygen. Manganese hydroxide, in turn, is obtained by the action of NaOH on MnSO4.
MnSO+2NaOH Mn (OH)2 Naz SO
2.Mn(OH), +0, >2MnO(OH),
MnO(OH), +H,SO, > MnSO, +2H,0+[0]
2KT+H,SO, +[O]> K,SO, + H,0+1,
2Na,S,0, +1, > Na,$,0, +2Nad
Starch+I Blue colored complex.
solution
The liberated iodine (la) is titrated against standard sodium thiosulphate (Na,S203)
indicator.
using starch as

Requirements:
(0.01 N),
dichromate sodium
of pH 7, standard potassium
Reagents and solutions: Standard buffer
conc. HSO,
alkali KI solution (KI + KOH in water),
thiosulphatesolution, 10% KI solution,
starch solution as indicator
manganese sulphate,

Beakers.
Apparatus: Conical flask, Burette, Measuring flask,

1
Procedure:
Titration 1: Standardization
of Sodium
Rinse and till the
burette with Thiosulphate
0.0IN KC,0, given sodium
solution (Bottle A) into thiosulphate solution (Bottle B).
ml (1/2 T.T.) of
10% KI, and titrate
a clean
conical flask. To this, add 5 m.Pipette out 20 ml of
straw yellow against sodium thiosulphate H,SO, (1/2 T.T),.5
colour, add starch indicator solution. When the solution becomes
bluish brown colour. and continue the
Repeat titration to get concordant titration. End point is the disappcarance of
the
value.

Titration 2: Estimation of
Dissolved Oxygen
USing a
measuring cylinder, add 100 mL of sample water in a conical flask.
MnSO and 2 mL of alkali KI Further, add 2 ml of
solution and shake well for the
the flask for few
minutes to allow the
rough mixing the reagents. Set aside
of
precipitate to settle down and then add 2 mL of conc. H,SO, for
complete dissolution of the precipitate. Then, titrate
the solution turn into
against std. sodium thiosulphate solution. When
light yellow, add starch indicator. End point is the disappearance of bluish
brown colour. Repeat the titration to
get the concordant value. Calculate the strength of dissolved
oxygen from the titer value. Based on that, calculate the amount of dissolved oxygen in the given
water sample.

OBSERVATION AND CALCULATIONS

Titration I: Standardization of Sodium Thiosulphate

Burette reading (mL)

Volume of sodium
S. No. Volume of K,Cr;0, (mL)
Initial Final thiosulphate (mL)

018.4 18.4
2
18.3 18.3
3
I6.4 18.4
Concordant value

Calculations:
Volume of potassium dichromate V 20 mL
0.01 N
Strength of potassium dichromate N
=

Volume of sodium thiosulphate V25........mL (From Titration 1)

-

Strength ofsodium thiosulphate N

=..? (To caclate)
V,N = V2N

N=V,N/V2
=20 x 0.01/V2
=.09O. N
Strength of sodium thiosulphate N,
=
Titration- 1l: Estimation of Dissolved Oxygen
Burette reading (mL) Volume of sodium
Volume of water sample
S. No.
Final
thiosulphate
(mL) Initial
(mL)

1.
0 12.9 12.9
12.8 12.8 ml
.
100

100 12. 2.8n

Concordant value
12.3

Calculation:
mL (From Titration -2)
ofsodium thiosulphate V, .
=

Volume
U.J08 N (From Titration 1 calculation)
Strength ofsodium thiosulphate N,
=

Volume of water sample taken V= 100 mL

?
Strength of given water sample N,
=

V,N=V,Na
N =V2 XN/100
-D.008N
*****

Amount of dissolved oxygen (ppm) = normality x equivalent weight of O,x 1000 mg/L of the given

water sample.
0.00136 Nx8x 1000 mg/
11. p p m .

Result: Amount of dissolved oxygen in the given water sample = . . . ppm.