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Microbial Growth
Foodborne illnesses
Conclusion
Introduction
Food microbiology encompasses the study of microorganisms, which have both
beneficial and deleterious effects on the quality, and safety of food.Food
microbiology focuses on the general biology of the microorganisms that are found in
foods including: their growth characteristics, identification, and pathogenesis.
Specifically, areas of interest which concern food microbiology are food poisoning,
food spoilage, food preservation, and food legislation. Pathogens in product, or
harmful microorganisms.
"The study of food microbiology has come a long way since its beginnings in the
19th century. Early pioneers in the field, such as Louis Pasteur and Robert Koch,
laid the groundwork for our understanding of foodborne pathogens and the role of
microbes in food spoilage.
MICROBIAL GROWTH
Introduction
In microbiology, a bacterial food source is referred to as the substrate. A
substrate may be in the form of meat, vegetables, debris on a countertop, soil on
your hands.Bacteria will reproduce wherever they can find substrates and convert
them into usable resources—as long as the surrounding environmental conditions are
suitable. By understanding the conditions under which bacteria reproduce, we can
begin to understand how we can either harness that growth to our advantage (as in
the production of cheese and wine) or inhibit the growth to prevent food spoilage
and foodborne illnesses.
Potentially hazardous food (PHF) is the term we use to describe a food substrate in
which microbes are able to survive, replicate, and colonize. The BC Food Premises
Regulation (Government of British Columbia, 1999) defines a potentially hazardous
food as a “food in any form or state that is capable of supporting
the growth of disease-causing microorganisms or the production of toxins.” Bacteria
can easily grow in some types of food—for example, raw fish and dairy products—
because there are plenty of nutrients and moisture in those foods.
Figure 1 — Bacteria growing on a nutrient agar plate

In Figure 1, bacteria are seen growing on a nutrient agar plate.
The growth of microorganisms like bacteria and fungi can cause food spoilage,
resulting in wasted food and financial loss.
NUTRIENT AVAILABILITY:
Nutrients availability. Microorganisms need certain nutrients, like carbohydrates,
proteins, and fats, to grow. The more nutrients a food contains, the more likely it
is to spoil.This is because the microorganisms use the nutrients to create energy
for themselves. So, when a food has a lot of nutrients, the microorganisms have a
lot of energy to grow quickly. This is why canned and processed foods often have a
longer shelf life than fresh foods. They have less nutrients, and are therefore
less likely to spoil.
Oxygen
Some microorganisms, like aerobic bacteria, need oxygen to grow. Other
microorganisms, like anaerobic bacteria, don't need oxygen to grow. The amount of
oxygen in a food product can affect which types of microorganisms can grow in.
FOOD PROCESSING:
Food processing is another method that is used to prevent spoilage. Food processing
can include methods like pasteurization, dehydration, and canning. These methods
can remove moisture, add preservatives, or otherwise alter the food product to make
it less susceptible to spoilage. Some methods, like canning, can also preserve the
food for a long time.
METHODS OF FOOD PROCESSING:
DRYING:Drying, or dehydration, removes the moisture from a food product. This makes
it harder for microorganisms to grow and cause spoilage. Drying can be done in a
number of ways, including air drying, sun drying, and freeze drying. Dehydration is
often used to preserve fruit and meat products.
Pathogenic Bacteria:which are bacteria that can cause disease in humans. Some
examples of pathogenic bacteria include Salmonella, Listeria, and E. coli. These
bacteria can contaminate food through a number of routes, including cross-
contamination, contact with animals, and contamination of food-processing
equipment.
Molds:which are a type of fungus that can grow on food. Some molds can cause food
spoilage and produce toxins that can be harmful to humans. However, some molds are
actually used in the production of certain foods, like blue cheese. And some molds
are used in the production of medicines and other products.
TECHNIQUES
Culture techniques:This is a way of growing bacteria in a controlled environment,
such as a petri dish. The bacteria can be grown on different types of media, such
as nutrient agar or blood agar. This technique allows scientists to study the
growth rate and other characteristic of the bacteria.
Molecular technique:which use tools like DNA sequencing and polymerase chain
reaction (PCR) to study the genetic makeup of bacteria. These techniques allow
scientists to identify the specific types of bacteria that are present in a food
sample, as well as the genes that they possess.
Another example:Is the use of PCR to detect the presence of E. coli O157:H7 in
ground beef. This is a type of E. coli that can cause severe illness. By using PCR,
scientists can quickly and accurately detect the presence of this bacteria in
ground beef.
YEAST FERMENTATION
Yeast fermentation is another type of fermentation that uses yeast instead of
lactic acid bacteria. Yeast fermentation is used to make bread, beer, wine, and
many other products. The yeast converts sugars into carbon dioxide and alcohol. The
carbon dioxide makes bread rise and gives beer its fizziness. And the alcohol gives
wine and beer their signature taste.

PROBIOTICS
Probiotics are live microorganisms that have health benefits when consumed in
adequate amounts. They're found in many fermented foods, like yogurt, kimchi, and
sauerkraut. Probiotics can help improve gut health, reduce inflammation, and even
boost the immune system.
Different types of probiotic microorganisms:
The two most common types are Lactobacillus and Bifidobacterium. Lactobacillus is
found in yogurt, sauerkraut, and kimchi. It can help improve digestion, reduce
bloating, and even fight off harmful bacteria. Bifidobacterium is found in yogurt
and fermented milk products. It can help with digestion, inflammation, and even
allergies.
Probiotics help to balance the microorganisms in the gut, which is called the gut
microbiome. They can help reduce the growth of harmful microorganisms like E. coli
and Clostridium difficile. They can also improve the digestive system by producing
enzymes that break down food. And they can even improve the immune system by
stimulating the production of antibodies.They can also improve the quality of food
by keeping it fresh for longer and improving its nutritional value.
SPOILAGE
Food spoilage is caused by a number of different factors, including temperature,
humidity, and the presence of oxygen. However, the growth of microorganisms is
often the main cause of spoilage. Bacteria and fungi can release enzymes and toxins
that cause food to deteriorate and develop off-flavors. For example, the bacteria
that cause spoilage in milk products produce enzymes that break down milk proteins
and cause the milk to curdle.
Types of food spoilage
Rancidity:is a process that causes fats and oils to become oxidized and develop
off-flavors. It can be caused by a number of factors, including exposure to oxygen,
light, and heat. Rancidity is often accompanied by the development of free
radicals, which can cause other chemical reactions that further deteriorate the
food.
Toxins:Certain microorganisms can produce toxins that can cause illness even after
the food has been cooked. The most common example of this is botulism, which is
caused by the toxin produced by Clostridium botulinum. This toxin is not destroyed
by cooking, so it's important to prevent its growth in food. Other examples of
toxins that can cause food poisoning include staphylococcal enterotoxin and shiga
toxin.
FOOD ATTRIBUTION
Food attribution is the study of finding out what types of foods are sources of
foodborne illness. One of the questions asked during food attribution research is,
“At what point in the food chain does a pathogen originate?”This often happens at
the farm level; where failure to control the pathogen occurs, contamination may
take place or be amplified during processing or at home.The most common method of
food attribution is called epidemiological investigation. This involves
interviewing people who are sick and gathering information about what they ate
before they got sick. The information is then analyzed to see if there is a pattern
that points to a specific food or ingredient as the cause of the illness.
The critical control points (CCPs) are the steps in a food production process where
hazards can be controlled. These are the points where the greatest risk of
foodborne illness exists. Each CCP should have a critical limit, which is the
maximum level of a hazard that is acceptable. For example, a critical limit for
pathogenic bacteria in a food product might be zero. If the critical limit is
exceeded, corrective action must be taken to bring the hazard back within the
acceptable range.
Control point:There are several different types of control measures that can be
used at critical control points. One type is preventive control, which involves
measures that are taken to prevent the hazards from occurring in the first place.
Examples of preventive control measures include proper cleaning and sanitation of
equipment, using pasteurized ingredients, and sourcing raw materials from reputable
suppliers. Corrective control is another type of control measure. It involves
taking action to correct a problem if the critical limit is exceeded.
Verification:is the process of checking to make sure that the critical control
points are being followed and that they are effective. This can be done by testing
for the presence of microorganisms or by reviewing records and monitoring systems.
FOOD FRAUD
Food fraud is the intentional substitution, addition, tampering, or
misrepresentation of a food product.Food fraud can have serious health and economic
consequences, and it is a growing concern in the food industry.
Food fraud can take many forms, but the two most common types are adulteration and
mislabeling. Adulteration is when a food product is intentionally diluted or
substituted with another substance, such as adding water to juice or using cheaper
ingredients in place of more expensive ones. Mislabeling is when a food product is
labeled in a way that is misleading or false, such as labeling a product as organic
when it is not.
METHODS OF FOOD FRAUD
There are many different methods that can be used to commit food fraud. One common
method is called "economically motivated adulteration," or EMA. This is when food
products are intentionally adulterated in order to increase profits. Another method
is called "pass-off," which is when a lower-quality food product is sold as a
higher-quality product. Food fraud can also be committed through mislabeling,
counterfeiting, or the addition of hazardous or prohibited substances. Another
specific method of food fraud is called "substitution." This is when a food product
is intentionally substituted with a similar, but less expensive, product. For
example, a fish product may be labeled as a more expensive type of fish than what
it actually is. Another common method is called "fraudulent origin." This is when a
food product is labeled as coming from a certain location or region when it
actually comes from somewhere else. This can be done in order to increase the price
of the product or to meet labeling requirements.
Food commonly subject to food fraud: There are a number of foods that are commonly
subject to food fraud. One example is olive oil. Olive oil is often substituted
with other types of oil, such as sunflower oil or soybean oil, in order to increase
profits. Coffee is another food that is commonly subject to fraud. In some cases,
low-quality coffee is labeled as high-quality coffee. Honey is another food that is
often subject to fraud. It is often diluted with water or sugar syrup in order to
increase profits.
CONCLUSION
In summary, food microbiology is the study of the microorganisms that are present
in food. These microorganisms can be beneficial, like those used in fermentation,
or they can be harmful, like those that cause foodborne illness. Food microbiology
helps to ensure the safety of the food supply, and it also plays a role in the
development of new food products. There are a number of methods for controlling
microorganisms in food, including sanitation, food processing, and food packaging.
Certification standards help to ensure that the food industry is meeting the
highest standards for food safety.