Antibody Generation Handbook [2022 edition]

www.genscript.com
Make People and Nature
Healthier Through Biotechnology
ABOUT US
GenScript Biotech Corporation (stock code: HK.1548) is a leading global provider of life
science research, development and manufacturing services. Rooted in solid gene synthesis
technology, GenScript has established four major platforms: life science service and product
platform, biomedical contract development manufacture organization (CDMO) platform, cell
therapy platform and industrial synthetic biological products platform.

Founded in 2002, GenScript established its R&D and manufacturing headquarters in Nanjing,
China in 2004. In 2015, GenScript was listed on the Main Board of the Stock Exchange of Hong
Kong, with legal entities in the United States, China, Hong Kong, Japan, Singapore, the
Netherlands and Ireland. It operated business in over 100 countries and regions worldwide,
providing quality, convenient and reliable services and products for more than 100,000
customers.

As of December 31, 2021, GenScript owned more than 5,200 employees worldwide, with over
40% of them holding a Ph.D. or master's degree. GenScript has a number of intellectual
property rights, including more than 180 granted patents and more than 670 patent applications,
as well as a high dense technical secrets.

With its mission of “making people and nature healthier with biotechnology”, GenScript is
committed to be one of the most trusted biotechnology companies in the world. As of December
31, 2021, GenScript’s services and products have been cited in over 65,600 peer-reviewed
international academic periodical articles.
HISTORY & MILESTONES

2014
Established Legend Biotech
(Cell Therapy Division)
Awarded the Commissioned
Research Institute Leadership
Award
2011
Established a new R&D and production
base in Nanjing

2004 Participated in the synthetic yeast

genome Sc2.0 project as the only
commercial entity selected
Introduced custom protein and Established Japanese subsidiary
antibody services Number of employees reached 1,000
Established research and production
facilities in Nanjing, China

2015
Listed on the Hong
Kong Stock Exchange
(stock code: HK.1548)

2009 2013
2002 Received investment from Established Bestzyme
KPCB China/The Balloch (Industrial Synthetic
GenScript established in New
Group Bioproducts Division)
Jersey, USA
 2019
New GMP Biologics CDMO R&D Center was in operation
2017 LCAR-B38M/JNJ-4528 was granted orphan drug status by
the FDA and priority drug status by the EMA
LCAR-B38M /JNJ-4528 U.S. Phase 1b /2 clinical data
Legend Biotech and Janssen Biotech entered achieved excellent performance of 100%ORR and 69%CR
into a global strategic partnership for BCMA
products
CFDA accepted Legend Biotech's IND
application
Acquired 100% shares of CustomArray and
obtained chip gene synthesis technology

2020 2021
2018 The Company jointly developed
the world's first neutralization
GenScript ProBio (Biologics
CDMO) became a leading CDMO
antibody detection kit with
BCMA Product received IND approval in in gene therapy and cell therapy in
Singapore to combat the
China and U.S. China through A round of financing
epidemic of COVID-19
BCMA program progressed well in China Legend Biotech Cilta-cel cell
and U.S. Legend Biotech was publicly therapy product submitted a
Biologics CDMO business unit officially listed on NASDAQ biologics license application (BLA)
established to the FDA and expected to be
approved by the end of February
2022
With more than 5,200 employees,
the group distributed life science
production capacity in Singapore
and the United States and cell
therapy production capacity in
Belgium to serve the global
market
Content

01 Antibody Service Introduction

Our Advantage
Antibody Development Process
01
02
Antibody Application 03

02 Antibody Service

Polyclonal Antibody
Monoclonal Antibody
07
11
Antibody Sequencing 23
Kit Development 25
Phosphorylated antibody 28
Antibody Production 30
Antibody Modification 31

03 Resource Center

Technical Resources 33
Bioinformatics Tools 34
FAQ 35
增值服务
Articles Published by Customers 37

04 Order Method and Order Query

Order Method 42
Order Query 42
 01
Antibody Service
Introduction
GenScript Antibody Services
Introduction to

Our Advantage
Antibodies are essential research tools that enable researchers in different scientific fields to define, identi-
fy, locate or quantify protein targets based on antibodies. As an award-winning supplier of customized
antibodies, GenScript has successfully delivered over 150,000 antibody projects. GenScript has animal
facilities certified by OLAW and AAALAC in which all rodents and rabbits can produce antibodies. From
antigen design to customized antibody production, our professional scientists and technical support team
will support your R&D projects!

More than 18 years of experience Delivery of 150,000+ antibody project

Serve for 5,000+ organizations Customers in more than 100 countries

110,000+ polyclonal antibody preparation project

14,000+ monoclonal antibody preparation project

400+ therapeutic lead antibody drug development project

Polyclonal antibody project 800-1000 antibodies/month

Monoclonal antibody project 80-100 antibodies/month

Antibody related projects 50 antibodies/month

01
 GenScript Antibody Services
Introduction to
Antibody Development Process

Antigen Animal Clone Antibody

Preparation Immunization Screening Purification
and Production
OptimumAntigenTM Proprietary immune Experienced hybrid- Antigen affinity
antigen design adjuvant library oma technology purification and
software independent- platform Protein A affinity
Immunized animals
ly developed by purification
like rabbits, rats, and B cell cloning platform
GenScript
mice can be chosen with high diversity and Antibody production:
High throughput high success rate Shake-flask produc-
6,000 m2 of animal
protein expression tion and ascites
house First introduction of
production
Fast delivery of 6 Beacon® single B cell
Certified by OLAW
working days with sorting platform to Transient and stable
and AAALAC
traceability facilitate antibody recombinant antibody
development at full expression
Customized protein
speed
expression

Flexible package
content

Cost-effective service

02
GenScript Antibody Services
Introduction to

Antibody Application

Protein detection and quantification

ELISA
Protein expression & interaction Customized monoclonal antibody
Western Blot & IP Antibody labeling

Customized monoclonal antibody

Customized polyclonal antibody
PolyExpress™ rapid polyclonal
antibody

Cell analysis
FACS

Customized monoclonal
antibody (MonoRabTM)
Fluorescence labeling

Protein localization
IHC

Customized monoclonal
antibody (MonoRabTM)
Fluorescent labeling

Protein identification and quantification

Mass Spectrometry

Customized monoclonal
antibody

03
 02
Antibody Service
GenScript Antibody Service

Antibody Service

Antibody Sequencing
Antibody variable region or full-length sequencing
High-throughput sequencing of variable regions

Monoclonal Antibody
Murine monoclonal antibody
MonoRabTM rabbit monoclonal antibody
Beacon® single B cell sorting platform

Polyclonal Antibody
Rapid polyclonal antibody
Customized polyclonal antibody

05
 GenScript Antibody Service
Kit Development Service
ADA ELISA kit development
HCP ELISA kit development
PK kit development
Development of micromagnetic particle
based chemiluminescence kit Phosphorylated antibody
Committed antibody ELISA titer ≥ 1:256,000
Cross-reaction with non-phosphorylated peptides < 10%

Antibody Production
Shake-flask production
Ascites production
Recombinant antibody preparation

Antibody Modification
Biotin labeling
HRP labeling
Fluorescein molecular labeling
Antibody fragmentation

06
GenScript Antibody Service

Polyclonal Antibody
Most antigen molecules have multiple epitopes, and each epitope can stimulate the body to produce
a specific antibody. After animals are immunized with antigens, the immune serum was collected and
purified to obtain polyclonal antibodies. Polyclonal antibodies recognize multiple epitopes of antigens
and detect multi-epitope antigens with good stability.

Polyclonal Antibody Service

Rapid polyclonal antibody
Customized polyclonal antibody

Effectiveness Animal Type Antigen Source Antigen Type Antibody Delivery

Rapid antibody Rabbit Provided by customers Peptide Antigen affinity purified

production (45 days) Peptides designed and antibodies
Rat Protein
synthesized by GenScript Pro A/G purified antibody
Mice Micromolecule
Expressed protein (total antibody)
Guinea pig designed by GenScript Plasmid DNA Antiserum

Goat Cell, etc.

Specialized production facilities

Certified by AAALAC and OLAW
6000 m2 well-equipped animal house with constant temperature and humidity
The experimental staff are professionally trained and experienced, and operate in strict accordance with SOP
A complete range of animals, including rabbits, mice, rats, guinea pigs, and SPF level of animals are available.

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 GenScript Antibody Service
Rapid polyclonal antibody

The PolyExpress™ Rabbit Polyclonal Antibody Service is a new rapid antibody preparation service from
GenScript that combines antigen design software, proprietary optimized adjuvants and rapid immunization
protocols. Our service can help customers complete polyclonal antibody production in 45 natural days with a
promise of final ELISA titer greater than 1:256,000 and a promise of positive Western Blot results for protein
antigens.

Service Advantages
Fast delivery with 45 days
More antibody delivery: Budget saving and more economy
Higher ELISA titer: titer >1:256,000, antigen affinity purification about 2.5 mg/ rabbit or 100-300 mg/ goat

We offer proprietary optimization technology, focusing on each step of polyclonal antibody production

Antigen Immunized Purified

Synthesis Animals Antibody

Intelligent design: Effective immunity: Guaranteed ELISA titer:

More rational antigen design greatly Serum titer is 2 gradients higher than 1:256,000, industry-leading
improves the probability of antibody that of Freund's adjuvant Guaranteed delivery:
application success Strict production: Meet the demand for experimental
Flexible service: Each step of the experiment was antibodies
Customers can provide antigens or carried out in strict accordance with Guaranteed cycle:
provide sequences for antigen SOP specifications, as the quality is
synthesis determined by the process Make sure the experiment proceeded
on schedule

Service Details
Scientific research makes it so convenient
Antigen Serum + Antibody

Antigen Immunized Antibody

Preparation Animals Purification

Peptide or proteins Rabbit ELISA titer: 1:256,000

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GenScript Antibody Service

Service Name Antigen Antibody delivery* Antigen Delivery Delivery Standard Cycle

ELISA titer
2 purified polyclonal
Cat SC1676 1 Protein 200 μg antigenic protein 1:256,000 10-11 weeks
antibodies (1.5-6 mg/rabbit)
WB positive

Antigenic peptide ≥ 2 mg ELISA titer

Cat SC1180 1 peptide 1 purified polyclonal antibody 45 days
≥ 2 mg 1:256,000

* Hint: Selecting proteins and multiple peptides designed based on different regions of proteins as antigens has a higher success rate of obtaining
antibodies matching antigen proteins.

Case:

GenScript provides Arr2 antibody for Western Blot to detect the effect of Osi21 knockdown on Rh1 and Arr2.

09
 GenScript Antibody Service
Customized Polyclonal Antibody

GenScript provides one-stop customized polyclonal antibodies solutions, from antigen design, antigen
preparation, animal immunization to antibody purification and QC testing (ELISA test, etc. ), offering you
with a full range of customized antibody services.
Our polyclonal antibody service covers rabbit, mouse, rat and other species. Antibody tests can be carried
out in AAALAC and OLAW international certified laboratories. We advocate humane and scientific treatment
of animals and ensure the quality of animal experiments.

Service Details

Antigen Immunized
Testing Purification QC Shipping
Preparation Animals

Antibody delivery Amount of Antibody Delivery Delivery Cycle

Antigen Delivery
Source (different ways of
Peptide Protein Standard (ELISA) Peptide Protein
antibody purification)
Antigen Antigen Antigen Antigen
Protein A affinity purified
200-500 mg 1:256,000 13 weeks
antibodies
GenScript
Welcome Welcome
synthetic antigen your enquiry
Antigen affinity purified 1-30 mg
(peptide/protein) your enquiry
antibodies (phosphorylat- (0.1 mg "Pan" 1:256,000 16 weeks
ed polyclonal antibody) antibody)

Antigen Antigen affinity purified 3-50 mg 1:256,000 11 weeks

(peptide/protein) antibodies
provided by
customer Protein A affinity purified 200-500 mg 1:256,000
antibodies 11 weeks

Pan antibody: Most protein families have different members. Different members may use the same motif or sequence, but different proteins have different
functions. Antibodies that recognize different members of the same protein family are called Pan antibodies.

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GenScript Antibody Service

Monoclonal Antibody
Monoclonal antibodies are highly homogeneous antibodies produced by a single B cell and directed only
against a specific antigenic epitope. Hybridoma technology, single B cell screening technology and phage
display technology are commonly used to obtain monoclonal antibodies. Hybridoma technology refers to
the fusion of B cells that produce specific antibodies and proliferative myeloma cells to form cells with
antibody-secreting function and permanent proliferation, so as to obtain monoclonal antibodies. Single B
cell screening technology refers to the direct screening of IgG secreting single B cells from the animal B
cell antibody library with advanced high-throughput DNA sequencing technology, such as the Next Genera-
tion Sequence (NGS), so as to obtain natural paired antibodies with high specificity and affinity.

Beacon® single
Murine hybridoma B Cell Cloning Platform B cell platform
Mouse: Cells of the
Antibody Cells of the spleen Antibody Antibody
Memory B cell spleen or lymph node
Source or lymph node Source Source
Rabbit: Memory B cell
Delivery of Cells and Delivery of Antibody and its
Products antibodies Products sequence Delivery of Antibody and its
Products sequence
Overall About 14 About 10
Overall Cycle
Cycle weeks1 weeks2 Overall
About 8 weeks3
Cycle
Price Low Price Medium
Price High

Calculation standard:
1: non-difficult target, rapid immunization, no additional immunization required, one step antibody discovery, hybridoma antibody production and
purification
2: non-difficult target, rapid immunization, no additional immunization required, one step antibody discovery, sequencing, antibody production and
purification
3: non-difficult target, rapid immunization, no additional immunization required, a chip, sequencing, antibody production and purification

Service Features
Rapid preparation of monoclonal antibodies and customized monoclonal antibodies
Three major platforms: hybridoma platform, B cell cloning platform, Beacon® single B cell sorting platform
High quality, the antibody titer up to 1:512,000

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 GenScript Antibody Service
Guaranteed Murine Monoclonal Antibody

To meet customer needs for monoclonal antibodies, GenScript rapid preparation service for monoclonal
antibodies allows clone delivery within 10 weeks. We guarantee to provide customers with multiple
positive clones and purified antibodies with high affinity through OptimunAntigenTM antigen design software
and independently developed adjuvants. For this service, we will guarantee the delivered antibodies with
positive WB results with immunogens.

Service Advantages

Fast delivery of supernatant of High quality antibody Production specification

a mother clone with 45 days

Service Details

Cell fusion and Subcloning and fixed Antibody expression

Animal Immunization
supernatant delivery virus delivery and purification
Conventional
monoclonal
antibody
Day 0 FCA Week 6-8 Week 9-10 Week 13-14 Week 15-17

Cell fusion and Subcloning and fixed Antibody expression

Animal Immunization Saving 6-8 weeks
supernatant delivery virus delivery and purification

Rapid
immunization
(MonoBoost)
Day 0 T-Max Week 2 Week 5-6 Week 10 Week 12-15

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GenScript Antibody Service

Number
Protein Antigen of immunized Customer Deliverables First
Guarantee Starting Material
Package animals Screening Delivery

1 WB positive clone for

immunogen
Accelerated By At least 1 WB positive GenScript generated 30 ELISA
MonoBoost clone for immunogen antigen positive Up to 5 hybridoma cell
supernatants for lines (2 vials/clone)
(SC2129) ELISA ≥ 1:64,000 Customer provided 3 8 wks
initial evaluation 2 mg purified mAb from
antigen
by client 1 clone
0.2 mg purified antigen
(if GenScript provides)

1 WB positive clone for

Classic At least 1 clone that GenScript generated 20 ELISA immunogen
is WB positive for antigen positive Up to 5 hybridoma cell
(SC2128)
immunogen supernatants lines (2 vials/clone)
Customer provided 3 18-25 wks
for initial
ELISA ≥ 1:64,000 antigen 2 mg purified mAb from
evaluation by
client 1 clone
0.2 mg purified antigen
(if GenScript provides)

Cases:

ImmunoPlus™ can greatly improve the immune response of antigens with 96% homology.

1 00 00 00
Hybridoma number

1 00 00 0

1 00 00

1 00 0

1 00
Traditional method GenScript high efficiency fusion

By comparing the traditional method (left) with the GenScript high-efficiency capacitor method (right), the GenScript
fusion method has higher electrofusion efficiency, which effectively enhances the library capacity and improves the
success rate of positive antibody screening.

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 GenScript Antibody Service
Customized murine monoclonal antibody

GenScript offers a comprehensive customized service for murine monoclonal antibodies including the animal
species, number of immunized animals, immunization method (rapid and traditional), screening method
(ELISA or FAC), and other stages to fully meet your needs for antibody downstream applications. GenScript
can also assist you in scaling up production when the developed antibody is on demand.

Multiple immunization protocol

Immunogen
Breaking immune tolerance by
preparation
ImmunoPlus™
Improving screening
technology and
success rate
Animal CD138 screening and enrichment,
Immunization high efficiency electrofusion

Cell fusion HTP flow cytometry

and screening Other high throughput screening
High throughput
binding capacity
screening
Subcloning and
monoclonal High throughput screening technology
selection

High affinity antibody screening, EC50,

Antibody Multiple identification
IC50, epitope classification, epitope
production and screening tools
mapping, pairing

Service Details

Phase Package selection Delivery Cycle

MonoBoost or traditional immunization methods 8-10 weeks
(increase by 6-8 weeks) (conventional
Phase I ImmunoPlus™ technology test report immunization)
Immunization DNA immunization 2-4 weeks
Animals Balb/ C mice, C57BL6 mice or rats (MonoBoostTM
Number of immunized animal immunization)

Indirect ELISA or Westen Blot assay

Phase II
Capture-ELISA
Cell fusion and Competitive ELISA 2 mL supernatant/strain 4-6 weeks
screening Flow cytometry
Additional monoclonal screening

Phase III Subclonal screening

Cryopreservation Hybridoma cell lines
Subclonal
Cell banking 5 mL subclonal supernatant 6-8 weeks
screening and
Roller bottle production Purified antibody
amplification Antibody purification

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GenScript Antibody Service

Case 1: Preparation of murine monoclonal antibody for FACs assay

Antigen: Peptide Application: FACS

<Serum titer> <Clone 1> <Clone 2>

Control cell line

Antigen
overexpressed
cell lines

Difficulty 1: The homology of immunogen is 100% with mouse Difficulty 2: Immunization with peptides, for FACs application

Proprietary ImmunoPlus™ technology to break immune tolerance and produce

murine monoclonal antibodies with high homologous antigens

Case 2: Preparation of murine monoclonal antibodies that recognize specific

phosphorylated sites
Immunogen: Dual phosphorylated peptide Test material: 4 different peptides Immunization protocol: MonoBoost™
3.0
2.5
2.0
Dual phosphorylated peptide
OD450 1.5
Single phosphorylated peptide 1
1.0
Single phosphorylated peptide 2
0.5
0 Non-phosphorylated peptide
Clone 1 2 3 4 5

Clone 1 & 2: Only recognized dual phosphorylated peptide

Clone 3: Recognized dual phosphorylated peptide and single phosphorylated peptide 1
Clone 4 & 5: Recognized dual phosphorylated peptide and single phosphorylated peptide 2

MonoBoost™ Immunization improves the success rate of

murine monoclonal antibody o recognize the PTM sites

Case 3: Improvement of hybridoma positive rate by plasma cell enrichment

Fusion with Number of
Project Number of plate Positive rate
initiating cells positive clone
CD138 + spleen cells 5 58 12%
Project I: Conventional
peptide immunization Spleen cells 5 5 1%

Project II: MonoBoostTM CD138 + lymph node cells 10 826 86%

protein immunization Lymph node cells 10 120 13%

CD138 + lymph node cells 7 190 28%

Project III: MonoBoostTM
protein immunization Lymph node cells 7 33 5%

CD138+ enrichment increases the positive rate of hybridoma,

which is suitable for difficult projects and projects with high diversity requirements

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 GenScript Antibody Service
MonoRabTM rabbit monoclonal antibody

Rabbit possess a unique B cell maturation and development mechanism, which is more likely to produce
strong immune response to small molecules or weak immunogenicity antigens. Therefore, it is easier to
obtain antibodies against specific modified sites, saccharides, haptens and small molecules through rabbit
monoclonal antibody technology. With its unique advantages, the rabbit monoclonal antibody has been
widely used in the field of life science, including basic research, diagnosis and treatment. GenScript
provides high affinity, specificity and diversity of rabbit monoclonal antibody services, covering two R&D
platforms (B cell cloning and single B cell sorting), to assist you in the R&D process at full speed!

Service Features

High affinity High specificity

Rabbit B cells can produce Rabbit monoclonal antibodies have

picomolar dissociation coefficient stronger ability to distinguish similar
(i.e., high affinity) during epitopes and better specificity
maturation, which is 10-100
times higher than rodents

Strong stability Diversity

The unique IgG structure of Compared with mice, rabbits have

rabbits enhances the stability of higher recognition coverage to
the antibody, which is better antigens and produce antibodies
than that by rodents with higher diversity

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GenScript Antibody Service

Customized rabbit monoclonal antibody

GenScript MonoRab™ rabbit monoclonal antibody service has a R&D platform with B-cell cloning 周期 and
single B-cell sorting as its core. Combined with the advantages of rabbit autoimmunity, we can provide
high quality rabbit monoclonal antibodies for a variety of difficult projects for your multiple applications.

An average delivery of 50 clones Cost-effective Multiple applications

Service Details (B cell cloning as an example)

Phase Description Timeline Deliverables

4 rabbits (express or Express: 5 - 6 weeks Antiserum report

I) Animal Immunization conventional immunization)
Conventional: 8 - 10 weeks Small scale pAb purification (optional)

Positive clones report

3 weeks 1 ml B cell supernatant delivery, up to 50
II) B Cell Clonin 1 round of B cell cloning clones (optional)
and Screening
Additional screening and increased clone
number delivery options available

Sequence reports
III) Antibody Sequencing & Sequencing of 5 customer-selected Up to 5 rAb supernatants (2 ml), or
Small Scale Transfection clones, followed by small scale rAb 3 weeks
purified rAb (0.1 mg)
expression
Additional sequencing and supernatant
delivery options available

IV) Recombinant Antibody Scalable expression of selected 3 weeks Sequencing report

Production recombinant rabbit mAbs Purified antibody

PBMC Enrichment & Expansion Screening & Recombinant

Animal Antibody Sequencing &
Isolation of positive Supernatant antibody
Immunization Small Scale Transfection
Antigen-positive B Cells Harvest production

Fast with 11 weeks

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 GenScript Antibody Service
Case 1:

Combined immunization with multiple transmembrane proteins

CLDN18.2 + cells CLDN18.2 - cells

count

count
count

Alexa Fluor 488-H Alexa Fluor 488-H Alexa Fluor 488-H

[Pre-immunized Serum] [Positive reference antibody] [4790# Rabbit immunized serum]

The positive reference antibody was used to compare the immunized results, which showed that the rabbit immunized
serum obtained by our combined immunization scheme was equivalent to the positive reference antibody.

Case 2: High specificity of monoclonal antibody against small molecule

Target small molecule Analogues 1 Analogues 2

Affinity: KD≈10-11M Cross reaction: < 0.0329% Cross reaction: < 0.0304%

The antibody affinity of the target molecule reaches 10-11, and its cross-reactivity with analogues is low.

Case 3: Recognized different antigen conformations for application in immunohistochemistry

Nature protein Denatured protein Peptide

53 20 19

53 ELISA positive clones Top 20 clones by affinity ranking 19 clones used for IHC

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GenScript Antibody Service

Single B Cell Sorting Platform

GenScript introduced Beacon® in early 2020 and became the first antibody R&D service company
using Beacon® in Asia Pacific. Beacon® is a single-cell sorting device based on integrated microfluidic
technology, signal detection system and photoinduced two-dimensional electrophoresis. The screen-
ing scope of single B cell sorting platform covers more than 90% B cells; in terms of screening time,
the screening with Beacon® is completed in only one day, saving 12 weeks compared with hybrid-
oma. Combined with the above advantages, the single B cell sorting platform is a superior choice for
large-scale B cell screening.

Service Advantages

Captured target clone Faster screening time Automated Higher diversity and affinity
with high probability screening process

Average cell Multiple antigen

Project
loading number Screening: 3-5 binding screening
management
per chip rounds/day team (Ph.D.)
> 10,000
Cell functional
screening

Average monoclones High throughput:

Project
per chip 4 chips screened Other customized experience: > 40
> 200 simultaneously protocols

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 GenScript Antibody Service
Service Details

Phase Ia Phase IIIb

Phase II Phase IIIa
Animal Phase Ib Recombinant
Beacon® cell Single B cell
immunization ASC enrichment antibody
screening sequencing
or PBMC (small scale)

Cycle: 5 days (rabbit) On Beacon®

Cycle: 4-6 weeks Cycle:7 days Cycle:4 days
/ 5 hours (mouse) Cycle:1 day

Three Phases of Single B Cell Screening:

Phase I: Animal immunization, serum detection and enrichment of ASC cells;
Phase II: Beacon® cell screening, including ASC cell introduction, chip assay and positive cell export;
Phase III: Single B cell sequencing, small-scale antibody expression.

Case 1:

OptoSelectChip,14k pens IgG Assay Antigen Assay

Secondary
Secreted antibody+Fluor
antibody

Secreted
antibody
Anti-IgG
bead
SA
Anti-IgG Anti-IgG
bead
+Fluor
Biotinylated
Antigen

5704 single B cells on chip 5704

534single
IgG positive
B cells on chip 12 antigen specific

(A) (B) (C)

Antibodies against transmembrane protein (murine)

(A) 5704 B cells were screened on the chip with Beacon®; (B) 534 IgG-positive clones were obtained by chip screening;
(C) 12 positive clones were obtained by antigen screening. In other cases, 21 antigen-specific clones were harvested
for glycosylated transmembrane proteins.

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GenScript Antibody Service

Case 2:

Immunized Animals with Collection of Single CD138 + Positive

Peptides Cells from the Spleen Cell Enrichment

Beacon® Beacon® Beacon®

24 IgG positive hits 1073 IgG Positive Clone Harvested 10,633 Single B Cells

Peptide Antigen (murine)

Peptides were applied as the antigen for animal immunization, then CD138 + cells were collected from spleen cells,
then 1073 IgG positive clones were harvested by Beacon® single B cell screening, and 24 antigen positive clones were
obtained by final antigen screening.

In addition to the above cases, GenScript also has other cases of monoclonal antibodies against COVID-19 N and S
proteins. The immunized animals include rabbits and mice, and the antigens include general proteins, peptides,
glycosylated transmembrane proteins, and GPI-linked glycoproteins.

Case 3: High affinity and diversity of rabbit monoclonal antibodies

against SARS-CoV-2 S protein

Epitope 1 BS-R2B12 BS-R2B16 BS-R2B30

Epitope 2 BS-R2B17 BS-R2B27 BS-R2B50

Epitope 3 BS-R2B2 4G6 12D3 39G6

Epitope 4 BS-R1B8

Clone
Name BS-R2B1 BS-R2B2 BS-R2B12 BS-R2B16 BS-R2B17 BS-R2B27 BS-R2B30 BS-R2B50 BS-R1B8 4G6 12D3 39G6

EC50
(ng/ml) 9.57 0.96 1.56 4.28 1.68 12.74 0.96 20.12 2.67 1.28 0.90 1.17

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 GenScript Antibody Service
Applications

Basic Research
The monoclonal antibody is widely used in the field of basic scientific research. It can be used to study the relationship
between the antigen structure and its function at different levels, like molecular, cell and tissue, and then to clarify its
mechanism theoretically. For example, fluorescent labeled monoclonal antibodies can be used as probes to convenient-
ly determine the location and distribution of biomacromolecules bound to them in cells.

ELISA Immunohistochemical FACS

Diagnostics
In vitro: Various In Vitro diagnostic (IVD) assays, such as immunohistochemistry, ELISA and radio immunoassays
(RI), are based on the principle of antigen and antibody binding.

In vivo: Visualization of monoclonal antibodies can be achieved by a variety of approaches; The most common
approach is to label monoclonal antibodies with radioactive or fluorescent molecules to help tracking monoclonal
antibodies in the body and quantifying the results.

Treatment
Anti-idiotype antibody: Pharmacokinetic (PK) is used to measure the absorption and excretion rates, distribution,
and half-life of a candidate small molecule or monoclonal antibody treatment. The results help to determine the
optimal dose and toxicity of the therapeutic molecule. Therefore, anti-idiotypic antibodies require sufficient sensitivity
to capture antibody targets either in free type, binding type or both two types in biological fluid samples.

Monoclonal antibody treatment: The high specificity and sensitivity of antibody to target antigen can both improve
the safety and reduce the toxicity, highly specific antibodies avoid off-target binding and provide safety guarantee;
highly sensitive antibodies require low doses of administration, further reducing the toxicity.

抗体药 独特型抗体
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GenScript Antibody Service

Antibody Sequencing
The antibody sequences are obtained by sequencing the hybridoma cells, which can avoid the inability of hybridoma
with negative transformation in long-term culture. Antibody sequencing plays an important role in antibody drug
development and antibody engineering.

GenScript provides monoclonal antibody sequencing services based on advanced antibody discovery platforms.
Monoclonal antibody sequencing includes conventional/sanger sequencing and high-throughput sequencing.

Service Advantages

High accuracy Rich experience Fast delivery within 10 High throughput

working days

Conventional Monoclonal Antibody Sequencing

01 02 03
mRNA reversely PCR amplified
Total RNA Isolation transcribed into the genes of heavy
first-strand cDNA chain and light chain

06 05 04
Amplified genes
Report preparation Sequencing cloned into the
results analysis sequencing vector

Service Content Delivery Time Deliverables

Final sequence report

Sequencing of Variable Regions Fast delivery within
10 working days Sequence raw data

Heavy chain and light chain sequence comparison file#

Plasmids containing antibody fragments sequence#

Full-length antibody sequencing service Fast delivery within
10 working days Expression vectors containing heavy and light chains#*

* The latest upgrade of monoclonal antibody sequencing service offers preferential price, speed improvement and promises no charge for
inaccurate sequencing
# Delivery of optional items requires additional fees
#* Expression vectors can be selected from GenScript list or provided by the customer

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 GenScript Antibody Service
High Throughput Sequencing

RNA extraction cDNA synthesis Library Sequencing Data analysis

/purification construction

Number of
Platform Starting materials Delivery Cycle
Clones
21-50
Final sequence report
Hybridoma: Cell suspension Cell
51-100 Expression vectors containing
Variable NGS 10 working days
precipitation/RNA/cDNA heavy and light chains# (additional
regions
101-200 (Miseq)
Single B cell charge and 1 week lead time
201-300 required)

# Deliverables are not a default and can be provided on your request at an additional cost

Category Additional service Delivery Cycle

Light and heavy chain expression vectors 10 μg research level expression vector 1 week

High throughput 1 mg recombinant antibody Guaranteed delivery 18 working days

sequencing Antigen binding confirmation
Laboratory report 5 working days
(contained in 1 mg recombinant antibody)
Subtype testing Subtype testing report 1 working day

Sequencing plasmid and QC file Plasmid and QC file /

Subcloned into expression vectors 10 μg research level expression vector 1 week

Light and heavy chain comparison Comparison file /

Conventional monoclonal
antibody sequencing Sequencing raw data File in ab1 format /

1 mg recombinant antibody Guaranteed delivery 18 working days

Antigen binding confirmation Laboratory report 5 working days

(contained in 1 mg recombinant antibody)
Subtype testing Subtype testing report 1 working day

Cases:
Type Subtype
Mice IgG, IgM, IgA Kappa/Lambda

Rat IgG, IgM Kappa/Lambda

Human IgG, IgM, IgE Kappa/Lambda

Rabbit IgG, IgM Kappa/Lambda

Hamster IgG Kappa/Lambda

Macaque IgG Kappa/Lambda

Alpaca IgG Kappa/Lambda

Transgenic animals IgG Kappa/Lambda

24
GenScript Antibody Service

Kit Development
With the development of biomedical research and the innovation of biotechnology, antibody drugs and other
biological agents have made remarkable progress in the treatment in a variety of diseases (including
cancer, autoimmune diseases, and inflammation). Therefore, the immunogenicity detection, pharmacoki-
netic detection and host cell protein residue detection of biopharmaceutical clinical research is particularly
important.

Leveraging advanced scientific research concept and strong development capacity, GenScript provides
customers with efficient and fast immunodetection kit development services based on advanced technology
and rich experience in antigen preparation, protein expression and purification, antibody preparation and
various immunoassays,.
The kit is used throughout the drug development and IND filling process

CMC Clinical phase I Clinical phase II Clinical phase III

Host cell protein /HCP testing New drug

application
Dose escalation and Concept validation and Massive efficacy
PK testing/Pharmacodynamics
preliminary PK dose determination verification and PK testing
/BLA
or
Animal toxicity experiment Immunogenicity study/ADA testing
NDA
PK / Pharmacodynamics study in special populations

Preclinical study Clinical study

Service Advantages

Fast Turnaround time Abundant production experi- Released above FDA One stop solution
9-week fast production from ence standards from research
antibody to kit >17 years of antibody development Precision: C.V.<10% grade to GMP
experience Meet the needs of
Substrate different applications

Substrate Substrate

Substrate Secondary Inhibited

antibody-drug antigen
conjugate
Primary
antibody-drug
conjugate Captured antibody
Antigen

Direct Indirect Sandwich Competitive Sandwich Competitive

[ELISA kit] [Micro magnetic particle
chemiluminescence kit]
GenScript provides excellent ELISA kit development platforms and experienced antigen and antibody development
systems, which can provide the ELISA kit development empowered by micro magnetic particle chemiluminescence
technology.

25
 GenScript Antibody Service
ELISA Micro magnetic particle
chemiluminescence
Testing signal Chromogenic product Optical signal

Sensitivity pg-ng/mL pg/mL

Linear coverage ＜104 ＞104

Device requirement Low High

Application Preferred in research Preferred in industry

Timeline 9-19 weeks

Service Details

Steps Original materials Deliverables Cycle

Preliminary determination of methods

Prove of concept Preliminary establishment of standard Periodic report 1-2 weeks
curve

Optimization of kit reaction conditions and

parameters
Assay development Determination of standard curve Periodic report 3-4 weeks
Stability evaluation, etc.

Determination of sensitivity
Measurement of intra-batch difference and
Assay validation Periodic report and 10 kits 3-4 weeks
inter-batch difference
Determination of recovery range
Determination of samples, etc.

Kit manufacturing* Provided kit Kit 2-3 weeks

* For more kits, you can order with an MOQ of 5 pcs.

Kit application

Agriculture diagnosis Quantitative detection of Food safety inspection

pesticide residues and drugs

Pharmacokinetic study Diagnosis of animal and Immunogenicity analysis

plant diseases

26
GenScript Antibody Service

Case 1:
PK kit development
PK kits developed by GenScript meet or exceed FDA requirements

Parameter Quantity FDA requirement

Testing range 0.5-24 ng/mL -

Sensitivity 0.25 ng/mL -

Precision - intra-batch difference ≤5% ≤20%

Precision - inter-batch difference ≤5% ≤20%

Recovery 80-120% 80-120%

Selectivity 80% 80%

Specificity Undetected -

Matrix Human serum -

Case 2:
HCP Kit Development HCP kits developed by GenScript exceed FDA requirements

Parameter Quantity FDA standard

CHO HCP detected by sandwich ELISA Testing range 1-100 ng/mL -

Sensitivity 0.608 ng/mL -

Precision - intra-batch difference ≤5% ≤20%

Precision - inter-batch difference ≤10% ≤20%

Recovery 85-115% 70-130%

Specificity Drug -

Matrix Solution for injection -

Preparation of HCP antibody: The preparation was

Case 3: conducted respectively according to molecular weight,
resulting in high antibody coverage
HCP Kit Development
Molecular weight
kDa
HMW LMW 95
72
HCP with molecular weight HCP with molecular
55
greater than 30 kD weight less than 30 kD
43

Respectively for antibody 34

preparation 26

Purified antibody
17
1. Antibody purity: > 90%

2. ELISA titer > 1:64,000

3. Recognition coverage of PAGE and WB 10

detection > 50% anti-HMW:LMW=2:1 anti-HMW:LMW=1:1

27
 GenScript Antibody Service
Phosphorylated antibody
GenScript provides customized phosphorylated protein-specific polyclonal antibodies and monoclonal
antibodies with a delivery success rate of more than 95%. Post-transcriptional modification of proteins,
especially phosphorylation of proteins, is involved in almost all cellular activities in the body, so the detec-
tion of phosphorylated proteins is crucial for the study of various developmental disorders and human
diseases. Our advanced antigen design tool OptimumAntigenTM, proprietary immune adjuvants, optimized
screening and purification technology of specific antibodies make our affinity purified phosphorylated
specific antibodies highly sensitive in detecting the phosphorylation of highly complex protein mixtures. In
addition to phosphorylated protein antibodies, GenScript also provides acetylation, methylation and other
modified protein antibody preparation services.

Service Advantages

Advanced antigen design High quality antibody Short delivery cycle

Service Details
Provided by
Service Content Deliverables Cycle
customers

First delivery
Advantages of rabbit monoclonal antibody

Final delivery
2-5 antibody hybridoma cell lines
Monoclonal antibody 2 mg purified antibody
(5 Balb/c mice or 3 rats) Target antigen/protein 2 mg phosphorylated peptide (<15 aa, 90% purity) First delivery
sequence 10-11 weeks
2 mg non-phosphorylated peptide (<15 aa, 90% purity)
ELISA, MS, HPLC report

Commitment
2-5 antibody hybridoma cell lines, at least one purified antibody
ELISA titer ≥1:256,000, cross-reaction with non-phosphorylated
peptides < 10%

Customized phosphorylated polyclonal Antigen provided by

antibody/monoclonal antibody service customers
Dependent on the content of the customized package

28
GenScript Antibody Service

Case 1:
ELISA test of polyclonal antibody against phosphorylated peptide

3
Coating phosphorylated peptides

Coating non-phosphorylated peptides

O D4 50

0
0 1 2 3 4
Concentration (ng /mL)

The affinity of phosphorylated peptide (EC50=157.5 ng/mL) was significantly

increased compared with that of non-phosphorylated peptide.

Case 2: ELISA test of murine monoclonal antibody against to

methylated peptide

3
Coating methylated peptides

Coating non-methylated peptides

2
O D4 50

0
0 1 2 3 4
Concentration (ng /mL)

The affinity of methylated peptide (EC50 = 9.6 ng/mL) was significantly

increased compared with that of non-methylated peptide.

Case 3: ELISA test of murine monoclonal antibody against to

acetylated peptide

3
Coating acetylated peptides

Coating non-acetylated peptides

2
O D4 50

0
0 1 2 3 4
Concentration (ng /mL)

The affinity of acetylated peptide (EC50 = 6.9 ng/mL) was significantly

increased compared with that of non-acetylated peptide.

29
 GenScript Antibody Service
Antibody Production
GenScript can provide large-scale antibody production services from microgram level to gram level for
in vitro diagnosis and preclinical antibody drug discovery. In order to further meet the various needs of
our customers, we can provide in vivo ascites production, in vitro shake-flask production, recombinant
expression and other antibody production services, including mycoplasma detection and clone develop-
ment with high-quality services. Certified by AAALAC and OLAW, GenScript ensures service quality at an
international level.

Service Features

In vitro antibody production In vivo antibody production Flexible scale-up

Service Details

Antibody production Ascites production Roller bottle production Recombinant production

Used in vitro for poorly Scalable

performing hybridoma cells in Scalable High lot-to-lot stability
Service features vitro culture (e.g. glycosylation, Lot-to-lot consistency Sequence optimized
low yield)
Highly customized

Volume 2-5 mL/mouse >1 L 1mL-200 L

30
GenScript Antibody Service

Antibody Modification
GenScript offers two kinds of modification services, antibody labeling and antibody fragmentation. The
antibody labeling services mainly include biotin, HRP and fluorescein molecular labeling. Biotin labeling is
a chemical reaction that covalently binds biotin to antibody molecules, while biotin labeled antibodies are
mainly used in protein chip analysis and immunohistochemistry experiments. Horseradish peroxidase
(HRP) is a commonly used enzyme in clinical and immunoassay, and HRP labeled antibody is widely used
in ELISA kits and Western Blot analysis. Fluorescein isothiocyanate (FITC) is a widely used luciferin at
present, and FITC labeled antibodies are commonly used in immunofluorescence and flow cytometry anal-
ysis. Labeled antibodies were used to perform qualitative, localized, or quantitative testing for the corre-
sponding antigen in different immunoassays.

Service Details

Modification categories Modification number

Biotin 、HRP 、FITC Up to 10 mg

Alexa Fluor™ 488、Alexa Fluor™ 568、Alexa Fluor™ 647、APC、PE Up to 5 mg

In order to meet the needs of more customers, GenScript also provides peptide antigen design, antiserum
preparation, ELISA titer determination, Western blot detection and other antibody preparation related
services. Equipped by our strong antibody service team, we provide customers with high quality antibody
supporting services efficiently.

Antibody Modification

Fluorescein Antibody
Biotin labeling HRP labeling fragmentation
molecular labeling
Immunochemical
Protein chip analysis ELISA kit Immunofluorescence technology
Immunohistochemical Western Blot analysis Flow cytometry assay Clinic diagnose and
test
treatment

31
 03
Resource Center
Antibody Resource Center

Technical Resources
White paper - GenScript's leading rabbit monoclonal antibody
www.genscript.com.cn
technology platform - MonoRabTM
This white paper is a technical resource for scientists who are in the antibody
engineering, therapeutics, and development areas of research and are working to
MonoRab™
GenScript's leading rabbit monoclonal antibody technology platform optimize their system.
White paper

You will learn from this white paper:

Advantages of rabbit monoclonal Application of rabbit monoclonal

antibody antibody
Features of MonoRabTM platform Cases

www.genscript.com.cn

White paper 1 - Hybridoma Technology for the Win!

This manual is designed to give you a deeper understanding of the process and
Victory of hybridoma technology
details of hybridoma.
Analysis of hybridoma antibody development technology

White paper 1

You will learn from this handbook:

Introduction of Host animal Hybridoma screening

hybridoma technology immunization & selection
Antigen design Cell fusion produced Monoclonal antibody
hybridoma purification

White paper 2 - Hybridoma Technology for the Win!

www.genscript.com.cn

This manual is a supplement to white paper 1, focusing on the advantages and

Victory of hybridoma technology characteristics of hybridoma technology.
Comparison of common antibody production platforms

White paper 2

You will learn from this handbook:

Introduction of hybridoma technology

Advantages of hybridoma technology in producing monoclonal antibodies
Selection of hybridoma technology
Misconceptions about hybridomas

33
 Antibody Resource Center
Bioinformatics Tools

A patented in vitro soluble and folding platform to recover purified proteins from
inclusion bodies produced by high protein expression, including high hydrostatic
FoldArtTM
pressure, small molecule additives and column folding technology to recover
high-purity soluble proteins.

GenSmart™ Codon Optimization technology is accessible and user-friendly. All

the key parameters are integrated into the algorithm, which can be completed by
GenSmart TM filling in the basic information (such as sequence and host), and customized
optimization of each gene leads to higher probability of obtaining functional and
active proteins.

The E.coli expression vector with unique solubility marker developed by

GenScript's professional scientists is specially used for the production of soluble
Solubility Tags
recombinant protein.
The success rate of E. Coli project is greater than 95%.

The advantage of OptimumAntigen ™ is to avoid unexposed epitopes, and aim to

OptimumAntigen™ have the required cross-reactivity to specific antigens with strong antigenicity, to

Antigen Design Tool identify the optimal coupling sites for the desired experiment, to apply the built-in
peptide synthesis and solubility course, and to ensure antigen immune response.

34
Antibody Resource Center

FAQ
To help you solve the problem more quickly, you can scan the following QR code to enter the antibody
resources page:

Questions Answers

Q: How can you get high-quality A: We will discuss this in terms of antibodies based on your application
antibodies? needs. Antibodies need to have sufficient specificity and affinity. One kind of
antibody may not meet all application needs, as different applications have
different requirements for antibodies.

Q: What antigens are available for A: Antigens can be generally classified as follows:
antibody development?
Peptide
Fragment or full length of recombinant protein
Natural protein (purified)
Whole cell
DNA antigen
Micromolecule
Antibody (anti-idiotype antibody)
VLP virus-like particles

Q: What is the fusion ratio between A: It's usually 2:1.

myeloma cells and spleen cells during
cell fusion?

Q: What animals can be used for

A: Rabbits, mice, rats, guinea pigs, goat, etc.
polyclonal antibody service?

Q: What factors should be considered in A: The antigenicity of peptides is greater than 0.6 (conventional); greater than
peptide antigen design? 1.0 (rapid)
Not difficult in peptide synthesis
Homology with immunized animals: Less than 90% for mice, and less than
95% for rabbits
Try to choose peptides with disorder value as antigens

35
 Antibody Resource Center
Questions Answers

Q: What is the difference between ascites A: The following aspects are included:
preparation and roller bottle production of
1. Ascites preparation
monoclonal antibodies?
BALB/C mice are used for ascites preparation to produce antibodies. Ascites
are prepared from rat hybridoma cells in nude mice. Ascites have a short
production cycle and a high yield, but the antibodies produced will contain
1%-10% of the mouse's own IgG.
2. Roller bottle production
Antibodies are produced by in vitro culture.
If there is a high requirement for experimental specificity and interference with
the later application of rat IgG, it is suggested to choose roller bottle production.
2-5 mg antibody can be obtained from ascites of a mouse, and 15-20 mg
antibody can be obtained from 1L roller bottle with SDS-PAGE purity above
90%.

Q: What antibody labeling services does A: HRP, FITC, Biotin, and other small molecule fluorescent dyes are available,
GenScript offer? and the customer is required to provide the name of the dye for our evaluation.

Q: How does GenScript use and store the A: It is suggested to store the antibody separately after receiving it, and it can be
antibodies we provide? stored at 4°C for 1 week; for long-term storage, it should be kept at -20°C or
-80°C.

Q: What is special about the adjuvant that A: GS adjuvant independently developed by GenScript has twice the immune
GenScript used? effect than Freund's adjuvant.

Q: What are the advantages of GenScript A: In the application of antibodies, affinity and specificity are the key points in
rabbit monoclonal antibodies? evaluating the usefulness of antibodies. Rabbit monoclonal antibodies are a
type of antibody with high specificity and affinity. KD of rabbit monoclonal
antibody mostly ranges from 10-9 to 10-11M, and some can reach 10-12M. In
addition, rabbit monoclonal antibody has higher diversity and is more likely to
obtain target antibodies. With its unique advantages, the rabbit monoclonal
antibody has been widely used in the field of life science, including basic
research, diagnosis and treatment. GenScript provides high affinity, specificity
and diversity of rabbit monoclonal antibody services, covering two R&D
platforms (B cell cloning and single B cell sorting). Preparation of rabbit
monoclonal antibody with B cell cloning of GenScript and 100% delivery of
antigen binding positive clones can promote your R&D process at full speed!

36
Antibody Resource Center

Literature Published by Customers

Title: Prokaryotic viperins produce diverse antiviral moleculesProkaryotic viperins produce diverse antiviral molecules

Journal: Nature IF: 43.07 (2021-01)

Doi：10.1038/s41586-020-2762-2

Introduction: DNA synthesis and cloning into pBad/His A was performed by Genscript.

Title: Central memory CD8+ T cells derive from stem-like Tcf7hi effector cells in the absence of cytotoxic differentiation

Journal: Immunity IF: 22.845 (2020-11)

Doi：10.1016/j.immuni.2020.09.005

Introduction: Tcf7GFP mice were injected sub-cutaneously (s.c.) at the base of tail with a modified synthetic long Ovalbumin peptide

(KKKKKLEQLEAAYSIINFEKL, termed KL-SLP) (15.86 nmole).(GenScript)

Title: Selenium-Doped Carbon Quantum Dots Act as Broad-Spectrum Antioxidants for Acute Kidney Injury Management

Journal: Adv Sci (Weinh) IF: 15.804 (2020-04-01)

Doi：10.1002/advs.202000420

Introduction: "Calnexin, Recombinant Calnexin, Rabbit anti-human, Mouse anti-human, Anti-Mouse IgG HRP.(GenScript etc)"

Title: Engineering Secretory Amyloids for Remote and Highly Selective Destruction of Metastatic Foci.

Journal: Adv Mater IF: 14.829 (2020)

Doi：10.1002/adma.201907348

Introduction: The amount of protein was quantified by WB using anti-His monoclonal antibody (Genscript).

Title: An ultra-stable cytoplasmic antibody engineered for in vivo applications.

Journal: Nat Commun IF: 12.124 (2020)

Doi：10.1038/s41467-019-13654-9

Introduction: HA tag (YPYDVPDYA)-fused scFv constructs (s3Flag-scFv-HA) were synthesised and codon-optimised for expression

in mice using Genscript.

Title: Optogenetic regulation of endogenous proteins.

Journal: Nat Commun IF: 12.124 (2020)

Doi：10.1038/s41467-020-14460-4

Introduction: Anti-RAS monobody NS1, referred in this paper as “iB(RAS)” was synthetized by GenScript, using amino acid

sequence from 5E95 Protein Data Bank entry for reverse translation53.

37
 Antibody Resource Center
Literature Published by Customers
Title: The natural function of the malaria parasite’s chloroquine resistance transporter

Journal: Nat Commun IF: 12.124 (2020-08)

Doi：10.1038/s41467-020-17781-6

Introduction: All of the host-derived peptides were custom-synthesised (GenScript; see Supplementary Data 1 and 2 for peptide

sequences).

Title: A possible universal role for mRNA secondary structure in bacterial translation revealed using a synthetic operon

Journal: Nature Communications IF: 11.878 (2020-09)

Doi：10.1038/s41467-020-18577-4

Introduction: SDS gels [Genscript] and transferred to a PVDF membrane using an E-blot protein transfer apparatus (Genscript).

Title: Forces during cellular uptake of viruses and nanoparticles at the ventral side.

Journal: Nature Communications IF: 11.878 (2020)

Doi：10.1038/s41467-019-13877-w

Introduction: Cells were fixed with 4% (w/v) paraformaldehyde in PBS for 20 min Samples were washed twice in PBS and for

membrane permeabilization cells were treated with 0.5% (v/v) Triton X-100 for 10 min Virusfactories were detected with an antibody

against the non-structural reovirus μNS protein (anti μNS rabbit IgG, 1:200, generated by GenScript,) and secondary antibody Alexa

Fluor 488 goat anti-rabbit IgG.

Title: Structure and dynamics of the active Gs-coupled human secretin receptor

Journal: Nature Communications IF: 11.878 (2020-08)

Doi：10.1038/s41467-020-17791-4

Introduction: This polyclonal antibody52 was raised by GenScript using the peptide antigen.

Title: On-demand drug release nanoplatform based on fluorinated aza-BODIPY for imaging-guided chemo-phototherapy

Journal: Biomaterials IF: 8.402 (2020-07-01)

Doi：10.1016/j.biomaterials.2020.120211

Introduction: Recombinant proteins were generated and obtained from GenScript.

Title: Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care

diagnosis

Journal: Biosens Bioelectron IF: 7.78 (2020)

Doi：10.1016/j.bios.2020.112573

Introduction: The T. cruzi-chimeric antigen IBMP 8.1 was obtained according to the methods reported by Santoset al. (2016a).

Briefly, the T. cruzi recombinant gene was optimized for Escherichia coli expression and synthesized by a commercial supplier

(GenScript).

38
Antibody Resource Center

Literature Published by Customers

Title: Soluble collectin-12 mediates C3-independent docking of properdin that activates the alternative pathway of complement

Journal: Elife IF: 7.725 (2020)

Doi：10.7554/eLife.60908

Introduction: DNA encoding human properdin was synthesized (GenScript) with the endogenous signaling peptide and a C-terminal

6xHis-tag and cloned into the pCEP4 mammalian expression vector using HindIII and BamHI restriction sites.

Title: Berberine Chloride is an Alphavirus Inhibitor That Targets Nucleocapsid Assembly

Journal: MBio IF: 6.956 (2020-06)

Doi：10.1128/mBio.01382-20

Introduction: ".Protein purity was confirmed by SDS-PAGE followed by Coomassie staining and Western blotting (WB) analysis

using polyclonal antibody (PAb) to the Strep tag (GenScript)."

Title: Risk1, a Phosphatidylinositol 3-Kinase Effector, Promotes Rickettsia typhi Intracellular Survival

Journal: MBio IF: 6.956 (2020-06)

Doi：10.1128/mBio.00820-20

Introduction: Codon-optimized recombinant proteins for wild-type (WT) full-length Risk1 (Risk1 WT), and the catalytically dead

mutant (Risk1 H297A) were expressed and purified by GenScript.

Title: A CD63 Homolog Specially Recruited to the Fungi-Contained Phagosomes Is Involved in the Cellular Immune Response of

Oyster

Journal: Front Immunol IF: 6.429 (2020)

Doi：10.3389/fimmu.2020.01379

Introduction: After incubating at 18°C for 2 h followed by three times of washing, 100 μl mouse anti-His tag monoclonal antibody

(Genscript) diluted to 1:2,000 was added and incubated at 37°C for 1 h.

Title: Calreticulin S-Domain Binds to Human Complement C1q to Interfere With C1q-Mediated Immune Functions

Journal: Front Immunol IF: 6.429 (2020-11)

Doi：10.3389/fimmu.2020.572326

Introduction: Endotoxin removal was confirmed using the ToxinSensor Endotoxin Detection System (GenScript).

Title: Properdin Is a Key Player in Lysis of Red Blood Cells and Complement Activation on Endothelial Cells in Hemolytic Anemias

Caused by Complement Dysregulation

Journal: Front Immunol IF: 6.429 (2020)

Doi：10.3389/fimmu.2020.01460

Introduction: Anti-properdin hybridoma cell lines were developed by Genscript usingour purified human properdin.

39
 Antibody Resource Center
Literature Published by Customers
Title: Receptor-Bound Perfluoroalkyl Carboxylic Acids Dictate Their Activity on Human and Mouse Peroxisome

Proliferator-Activated Receptor γ

Journal: Environ Sci Technol IF: 6.198 (2020-07-01)

Doi：10.1021/acs.est.0c02386

Introduction: Human and mouse PPARγ 138 ligand binding domains (LBDs) were prepared by Genscript.

Title: Extracellular Histones Inhibit Fibrinolysis through Noncovalent and Covalent Interactions with Fibrin

Journal: Thromb Haemost IF: 6.094 (2020-11)

DOi: 10.1055/s-0040-1718760

Introduction: Anti-his tag anti- bodies (A00186) were from Genscript .

In terms of services and products, GenScript have been cited nearly 10,000 times by more than 1300
journals of biomedicine such as Cell, Nature, Science and PNAS.
Please review literatures published by customers:
https://www.genscript.com/reference_peer-reviewed_literature.html。

Molecular Biology Services

8000
Catalog Products
7000
6000 Peptide Services
5000 Protein Services
4000 Antibody Services
3000
Oligo Services
2000
1000 Bioinformatics Tools
0 Gene Editing Services
2010 2011 2012 2013 2014 2015 2016 2017 2018 2019 2020 2021
COVID19

Number of Literatures cited GenScript from 2010 to 2021 Proportion Distribution of GenScript
Products and Services in the Literature

40
 04
Order Method
and Order Query
 Order Method and Order Query
Order Method

Email US: You can send your demand information to antibody@genscript.com

Call US: USA: +1-732-885-9188

Netherlands: +31 (0) 71 569 0120
United Kingdom: +44 (1865) 679988
Singapore: +65 3159 1898
Japan: +81-3-6811-6572
Korea: +82-10-9311-9208

Order Query

How to query? Log on GenScript website

1. Log in to your GenScript account
2. Click Account Name - User Center User Center
3. Click "My Order/Query" in the taskbar on the left of the page
4. Select "All Types of Orders” in the Order Type My Order/Query

5. Click the order number to enter the "Order Details" page to view the
order progress. For delayed or difficult orders, please email us for Select Service

consultation and confirmation. We will reply and follow up as soon as

possible. Click the Order Number

For delayed or difficult orders, please email us for consultation and

confirmation. We will reply and follow up as soon as possible.

42
Memo No.
Date
GenScript has always been committed to meeting the needs of its customers and
to bringing advanced technology to millions of laboratories.

For more events,

please follow us on “GenScript Reagent Service”.