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    Mini PCR Dog Genetics Webinar - Student Worksheet

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    nperales32
    The document discusses dog genetics and the RSPO2 gene which determines whether a dog has furnishings or not. It provides information about alleles, genotypes and phenotypes as they relate to this gene. It also includes Punnett squares and gel electrophoresis results to determine genotypes and predict phenotypes of dog puppies.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    Mini PCR Dog Genetics Webinar - Student Worksheet

    Uploaded by

    nperales32
    27 views4 pages
    The document discusses dog genetics and the RSPO2 gene which determines whether a dog has furnishings or not. It provides information about alleles, genotypes and phenotypes as they relate to this gene. It also includes Punnett squares and gel electrophoresis results to determine genotypes and predict phenotypes of dog puppies.

    Original Description:

    Molecular Diagnostics Assignment - “Help”

    Original Title

    Mini PCR dog genetics webinar_student worksheet

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    © © All Rights Reserved

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    PDF, TXT or read online from Scribd

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    The document discusses dog genetics and the RSPO2 gene which determines whether a dog has furnishings or not. It provides information about alleles, genotypes and phenotypes as they relate to this gene. It also includes Punnett squares and gel electrophoresis results to determine genotypes and predict phenotypes of dog puppies.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    27 views4 pages

    Mini PCR Dog Genetics Webinar - Student Worksheet

    Uploaded by

    nperales32
    The document discusses dog genetics and the RSPO2 gene which determines whether a dog has furnishings or not. It provides information about alleles, genotypes and phenotypes as they relate to this gene. It also includes Punnett squares and gel electrophoresis results to determine genotypes and predict phenotypes of dog puppies.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    https://www.youtube.com/watch?

    v=TPbGFTzGJew&t=19s

    Dog Genetics Webinar

    Review
    1. What does it mean for a dog to have furnishings?

    2. What gene determines whether a dog has furnishings?

    3. What is an allele?

    4. Describe the genetic difference between the two alleles of the RSPO2 gene.

    5. Is the presence of furnishings dominant or recessive?

    6. Summarize the relationship between genotype and phenotype.

    7. Fill in the table below using the following words and phrases: no furnishings,
    furnishings, FF, ff, Ff
    Phenotype Possible genotype(s)

    8. Why is gel electrophoresis a good tool to differentiate between alleles for the
    furnishings gene?

    9. Use the gel below to determine each dog’s RSPO2 genotype

    Dog RSPO2
    Genotype

    ©miniPCR bio 2020, all rights reserved ∙ www.minipcr.com


    Critical thinking
    Use the following information to answer the questions in this section:
    Dog RSPO2 genotype

    Molly Ff

    Zeus FF

    Otto Ff

    1. Assume Zeus is the father of Molly’s puppies.


    a. Fill in the Punnett square for a cross between Molly and Zeus

    Molly
    (labradoodle)

    Zeus
    (Poodle)

    b. What are the expected genotypic ratios?

    c. What are the expected phenotypic ratios?

    Continued on next page

    ©miniPCR bio 2020, all rights reserved ∙ www.minipcr.com


    2. Assume Otto is the father of Molly’s puppies.
    a. Fill in the Punnett square for a cross between Molly and Otto

    Molly
    (labradoodle)

    Otto
    (Labra-
    doodle)

    b. What are the expected genotypic ratios?

    c. What are the expected phenotypic ratios?

    3. Use the illustration of the electrophoresis results to determine each puppy’s


    RSPO2 genotype. Then, use that information to predict whether each puppy will
    have furnishings or not once their fur grows longer.

    Puppy Genotype Phenotype

    Astro

    Buster

    Chewy

    Daisy

    Elsa

    Flora

    ©miniPCR bio 2020, all rights reserved ∙ www.minipcr.com


    Ginger

    Hugo

    4. Is Zeus the Poodle or Otto the Labradoodle is the father of Molly’s puppies?
    Explain your reasoning.

    ©miniPCR bio 2020, all rights reserved ∙ www.minipcr.com

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