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    2.2 - Magnification and Calibration

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    jpvnym85gd
    The document discusses microscopy concepts including magnification, resolution, contrast, diffraction, calibration, and using a graticule and stage micrometer to measure samples. Formulas and examples are provided for calculating magnification and object sizes seen through the microscope.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    2.2 - Magnification and Calibration

    Uploaded by

    jpvnym85gd
    18 views8 pages
    The document discusses microscopy concepts including magnification, resolution, contrast, diffraction, calibration, and using a graticule and stage micrometer to measure samples. Formulas and examples are provided for calculating magnification and object sizes seen through the microscope.

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    2.2 - Magnification And Calibration

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    The document discusses microscopy concepts including magnification, resolution, contrast, diffraction, calibration, and using a graticule and stage micrometer to measure samples. Formulas and examples are provided for calculating magnification and object sizes seen through the microscope.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    18 views8 pages

    2.2 - Magnification and Calibration

    Uploaded by

    jpvnym85gd
    The document discusses microscopy concepts including magnification, resolution, contrast, diffraction, calibration, and using a graticule and stage micrometer to measure samples. Formulas and examples are provided for calculating magnification and object sizes seen through the microscope.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 8

    Thursday 14th September 2023 Vg

    Magnification ! Resolution !

    How many times The ability to distinguish


    larger an image between two separate points
    appears in (the smallest distance
    comparison to the between two points at which
    real image! they can be seen as separate)

    Resolution of the light microscope !

    • Resolution of the light microscope is limited by


    wavelengths of light !
    • The maximum is 200 um !

    Resolution of the electron microscope !


    The electron microscope has a much higher
    resolution due to its resolution power being
    much higher!

    We use this triangle when


    1
    A M
    S working out the image, actual
    and magnification of samples!
    Pg.17/18 Exam Questions ! HOMEWORK

    1. Eyepiece Graticule and Stage Micrometer.

    2. 20 = 4 5 um
    .

    Igrat 10YM=

    p 5 X10 4 75 YM
    =
    P5UM/20 =
    .
    .

    = 4 75X
    .

    3a. 14

    3b.
    14

    1 11
    .
    x 4 75.

    . 16 X 4 75
    2 ·

    . I4X4 75
    3
    ·

    =
    52 25 UM
    . =
    76YM = 66 50 YM
    .

    3c.
    52 25 + 76 + 66 50
    . .

    =
    64 PYM.

    3d. The eyepiece graticle has to be calibrated so we


    can measure accurately at varying magnifications
    + this is necessary to calculate real object
    size seen!
    3. Contrast = the varying shades of two objects.
    Resolution = The ability to distinguish between two
    points.

    4. 23mm 66um
    ,
    Mag =
    Image/actual
    ↓
    23000UM
    23000/66 =
    348x Iwluncertainty

    5. Diffraction can cause rays of light to spread out in


    many other directions than when it may have
    entered a medium. Due to this random spread of rays,
    some may cross over each other which can affect
    how we see images - blurriness. WVV
    + individual
    objects not separate.
    6. Graticule scales are different for each lens that you
    use and we also have a stage micrometer which has
    units on it as well.

    Friday 22nd September 2023 ⑤


    --

    Calibration: the conversion of graticule units into real


    units.
    1mm = 42 GU
    1000 UM = 42 GU Our microscope’s
    1G.U = 1000/42 = 23.8 um calibration

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