chapter 8 (glycolysis) أسئلة السنوات

General Biochemistry
Quiz 8 Glycolysis
1) How many H2O molecules are produced by glycolysis?

a) 1 b) 2 c) 3 d) 4
Answer: B, H2O is released when 2-phospoglycerate is converted to phosphoenolpyruvate and this step happens
2 times in glycolysis of 1 glucose molecule.
2) The enzyme that catalyzes the phosphorylation of glucose in glycolysis is:

a) Hexokinase b) Phosphofructokinase 1 c) Glucokinase d)Hexokinase and glucokinase
Answer: D, Tip: any enzyme that ends with “kinase” is a phosphorylation enzyme. Phosphofructokinase
phosphorylates F6P not glucose.
3) GLUT-1 transporter is found in which cells?

a) Leukocytes b) Erythrocytes c) Muscles d) Liver
Answer: B, muscles(and adipose) have GLUT 4(insulin dependent), and liver has GLUT 2.
4) Which of the following can be transported by GLUT-2 in mucosal intestinal cells?

a) Glucose b) Fructose c) Galactose d) All
Answer: D, GLUT 2 transports all types of sugar, GLUT 5 is fructose only, SGLT-1 is for galactose and glucose.
5) Phosphofructokianse-1(PFK-1) is activated by:

a) Fructose1,6-bisphosphate b) AMP c)Citrate d)ATP
Answer: B, PFK-1 is activated by fructose2,6-bisphoshate and AMP.
6) Which of the following stimulates pyruvate kinase?

a) Glucagon b) Glucocorticoids c) Fructose 2,6- bisphosphate d) Fructose 1,6- bisphosphate
Answer: D, pyruvate kinase is stimulated by Fructose 1,6-bisphosphate and insulin.
BRIEF team Page 1

7) Glycolysis takes place in which part of the cell?
a) Mitochondrial Matrix b) Intermembrane space c) Nucleus d) Cytosol
Answer: D
8) The enzyme involved in converting glyceraldehyde 3-phosphate to 1,3-BPG is:

a) glyceraldehyde 3-phospahte carboxylase
b) glyceraldehyde 3-phosphate dehydrogenase
c) phosphoglycerate kinase
d) phosphoglycerate mutase
Answer: B
9) The rate limiting step of glycolysis is(IMPORTANT)

a) Hexokinase
b) PFK-1
c) Pyruvate Kinase
d) PFK-2
Answer: B
10) Glucose 6-phosphatase is found in?

a) Hepatocytes(Liver)
b) Erythrocytes
c) Alpha cells of pancreas
d) Beta cells of pancreas
Answer: A, this enzyme produces glucose for the blood, and the liver produces glucose so it’s found in liver.
11) How many NADH will be produced by anaerobic glycolysis?

a) 4 b) 2 c) 1 d) 0
Answer: B, one glucose molecule produces 2 NADH during glycolysis.
12) Which of the following is NOT true about Glucokinase?

a) activated by translocation to nucleus
b) the sugar specificity of the enzyme is similar to that of other hexokinases.
c) predominate in B cells of pancreas
d) has higher Km and Vmax than other hexokinases
Answer: A, GK is activated when it’s out of the nucleus, other choices are true.
BRIEF team Page 2

13) cAMP hydrolysis to 5’AMP(AMP) is carried out by:
a) G-protein
b) cAMP Phosphodiesterase
c) cAMP Adenylyl cyclase
d) cAMP UCPs
e) Protein kinase A
Answer: B, adenylyl cyclase transforms ATP to cAMP, phosphodiesterase transforms cAMP to AMP.
14) The products of the pathway from glucose to lactate are:

a) 0 ATP 2 Lactate
b) 1 ATP 1 Lactate
c) 0 ATP 1 Lactate
d) 2 ATP 2 Lactate
Answer: D, 2 ATP from glycolysis and 2 lactate from each pyruvate molecule.
15) Hexokinase has higher affinity to glucose because of higher Km.

a) True b) False
Answer: False, higher Km = less affinity.
16) PFK-1 deactivated/inhibited by:

a) AMP b) Fructose 2,6- bisphosphate c) ATP d) A+B
Answer: C, PFK-1 is deactivated by ATP and citrate, and activated by AMP and Fructose 2,6- bisphosphate.
17) In the formation of PEP(phospoenolpyruvate), which of the following occurs?

a) production of H2O
b) consumption of H2O
c) NAD+ TO NADH
d) Production of ADP
e) Production of ATP
Answer: A
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18) Phosphoglucose isomerase enzyme forms:
a) Fructose-6-P or Glucose-6-P
b) NADH
c) Glucose 6 P
d) Fructose 6 P
e) ADP
Answer: A, this enzyme catalyzes reversible reaction between F6P and G6P
19) Which of the following molecules is the substrate for the aldolase enzyme?
a) Pyruvate
b) Aldose hexose sugars
c) Oxaloacetate
d) Phosphoenol pyruvate
e) Fructose 1,6- Bisphosphate
Answer: E
20) Which of the following enzymes in the glycolysis pathway does NOT involve ATP?
a) Glyceraldehyde-3-p-dehydrogenase
b) Pyruvate kinase
c) Phosphoglycerate kinase
d) Glucokinase
e) Hexokinase
Answer: A, Tip: all other enzymes are “kinases” which adds pho sphate to a molecule so they require ATP.
BRIEF team Page 4

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General Biochemistry

1) How many H2O molecules are produced by glycolysis?

2) The enzyme that catalyzes the phosphorylation of glucose in glycolysis is:

3) GLUT-1 transporter is found in which cells?

4) Which of the following can be transported by GLUT-2 in mucosal intestinal cells?

5) Phosphofructokianse-1(PFK-1) is activated by:

Answer: B, PFK-1 is activated by fructose2,6-bisphoshate and AMP.

6) Which of the following stimulates pyruvate kinase?

Answer: D, pyruvate kinase is stimulated by Fructose 1,6-bisphosphate and insulin.

BRIEF team Page 1

8) The enzyme involved in converting glyceraldehyde 3-phosphate to 1,3-BPG is:

9) The rate limiting step of glycolysis is(IMPORTANT)

10) Glucose 6-phosphatase is found in?

11) How many NADH will be produced by anaerobic glycolysis?

Answer: B, one glucose molecule produces 2 NADH during glycolysis.

12) Which of the following is NOT true about Glucokinase?

BRIEF team Page 2

14) The products of the pathway from glucose to lactate are:

15) Hexokinase has higher affinity to glucose because of higher Km.

Answer: False, higher Km = less affinity.

16) PFK-1 deactivated/inhibited by:

17) In the formation of PEP(phospoenolpyruvate), which of the following occurs?

BRIEF team Page 3

BRIEF team Page 4

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