Chapter#5 Variety of Life
Chapter#5 Variety of Life
Chapter#5 Variety of Life
PGC Bhakkar)
WHAT IS SPECIES?
A species is a group of natural population which can interbreed freely among each
other and produce fertile offsprings similar to themselves, but are reproductively
isolated from all other such groups in nature.
CHARACTERISTICS OF A SPECIES:
Each species possessed its own distinct structural, ecological and behavioral characteristics.
Species are independent evolutionary units.
Different species do not exchange genes between them.
CLASSIFICATION:
Biologists have classified all living things into groups showing similarities. All organisms are
related to one another at some point in their evolutionary histories. However, some organisms are
more closely related than others.
Example: Sparrows are more closely related to pigeons and both are different from insects.
NEED OF CLASSIFICATION:
There is huge diversity of organisms on planet earth. One and a half million species of animals and
over a half million species of plants are known.
To understand such a large collection of organisms with dissimilar forms, classification is needed.
Initially the classification was based on the appearance or morphology of plants and animals
For example we could classify flowering plants according to their colour, height, or any other
character. This type of classification is not important because it does not provide any information
about the basic differences and similarities among different individuals. But with advancement in
the knowledge of cytology, comparative biochemistry, physiology, genetics and molecular biology
the classification of organism has been modified.
LEVELS OF CLASSIFICATION:
Levels of classifications start from larger groups such as kingdom and are divided into smaller
groups upto species level.
Kingdom: Since long the living things are divided in two kingdoms: plants and animals.
Phylum/Division: kingdom is divided into smaller groups called phyla (also divisions for
plants, algae and fungi).
Class: A phylum is divided into classes.
Order: Classes are divided into orders.
Family: An order is divided into families.
Genus: A family contains related genera
Species: A genus is composed of one or more species. Species is the basic
unit of classification.
CLASSIFICATION OF CORN:
Zea
NOMENCLATURE
The process of giving the scientific names to organisms which consists of two words, the genus and
species, is called binomial nomenclature.
DISCOVERY:
Carlous Linnaeus (1707-1778), a Swedish botanist during the 18th century, devised a system
for naming and classifying all the organisms known to him.
The first word of scientific name is genus and always begins with a capital letter. The second word
is species which follows the generic name and begins with small letter.
The same generic name for potato and brinjal relects close relationship between these two-species.
Common names have no scientific basis. Common names had long caused confusion. From the
earliest times plants and animals have been given common names by the people. Since no system
was used in choosing common names, in many cases, various regions had their own
names for the same plant or animal.
Onion: If we take the example of onion, the common urdu name is „Piyaz‟ but in different
regions of Pakistan it is also known as „ganda‟ or „bassal‟ or „vassal‟. In different countries it
would have another set of names.
Amaltas: has also different common names as argvad, gurmala, golden shower, purging
cassia. Thus the same plant may have diferent names.
Blue Bell: Dozen of plants with bell shaped flowers are called „blue bells‟.
Black Bird: The word „black bird‟ would mean a crow as well as a raven.
Unrelated names:
To a biologist, a fish is a vertebrate animal with a backbone, fins and gills. But the word fish is
attached with unrelated animals such as „silver fish‟ which is an insect, and a „cray fish‟, „jelly fish‟
and „starish‟ are all invertebrates and do not fit the biologist‟s definition of a fish.
For centuries, the living organisms have been classified into two kingdoms, plants and animals.
Plants (Autotrophs):
Plants can prepare their own food from simple inorganic material and store energy and are called
autotrophs. Bacteria were also included in plants.
Animals (Heterotrophs):
Animals can not synthesize their own food from simple inorganic material and depend for their
food either on autotrophs or on decaying organic matter and are also known as heterotrophs.
Protista:
In 1866, Ernst Hackel proposed a third kingdom protista to accommodate Euglena like organisms
and bacteria. Many biologists found this system satisfactory.
1. Euglena:
Some biologists disagreed with three kingdom system for many unicellular organisms like
Euglena that have both plant like (presence of chlorophyll) and animal like (lack of cell wall)
characters.
2. Difference between prokaryotic and eukaryotic cells:
It ignores the differences between prokaryotic and eukaryotic cells.
3. Fungi:
Some biologists also disagree about the classification of fungi, such as bread mold, yeast
and mushrooms, which resemble plants in many ways but are not autotrophs. Fungi are
special forms of heterotrophs that obtain energy and structural material by breaking down
(decomposing) and absorbing food substances from the surroundings, and possess chitin as
a major structural component in their cell walls.
The five kingdom system, was proposed by Robert Whittaker (1969). It is relatively recent system
of classification.
Kingdoms:
Cellular Basis:
This system of classification is based on three different levels of cellular organization.
1. Unicellular prokaryotes.
2. Unicellular eukaryotes.
3. Multicellular eukaryotes.
Modes of Nutrition:
1. Photosynthesis (autotrophs).
Plants are autotrophic in nutritional mode, making their own food by photosynthesis such
as mosses, ferns, lowering plants.
2. Absorption (decomposers/reducers).
Fungi are heterotrophic organisms that are absorptive in their nutritional mode. Most fungi
are decomposers that live on organic material, secrete digestive enzymes and absorb small
organic molecules which are produced by digestion.
3. Ingestion (heterotrophs).
Animals live mostly by ingesting food and digesting it within specialized cavities. They lack
cellulose and show movements for example birds and reptiles.
E-Chatton (1937):
He suggested differentiating terms procariotique (from Greek pro, meaning before, and karyon,
meaning nucleus) to describe bacteria and blue-green algae, and the term eu-cariotique (from
Greek eu, mean true) to describe animal and plant cells.
They modified five kingdom classification of Whittaker by considering cellular organization, mode
of nutrition, cytology, genetic and organelles of symbiotic origin (mitochondria , chloroplast).
VIRUSES
DEFINITION:
The word virus is derived from Latin word venome which means poisonous fluid. Viruses can be
defined as non cellular infectious entities which contain either RNA or DNA, normally encased in
proteinaceous coat, and reproduce only in living cells. Viruses utilize the biosynthetic machinery of
the host for its own synthesis and then transfer themselves efficiently to other cells.
VIROLOGY: The branch of biology which deals with the study of viruses is known as virology.
HISTORY:
About a century ago at the time of Louis Pasteur (1822-1895) and Robert Koch (1843-1910), the
word “virus” was generally referred to as a poison associated with disease and death. Now viruses
are recognized as particles of nucleic acid often with a protein coat.
DISEASES OF VIRUSES:
Some viral diseases have been known for centuries. Viruses cause many diseases such as influenza,
hepatitis, small pox and AIDS.
HISTORY OF VIRUSES:
Vaccination of James:
In 1796, Edward Jenner removed the material from cowpox lesion on the hand of milkmaid and
injected it into 8 years old boy James. After six weeks the boy was inoculated with pus from a small
pox victim, but the boy did not develop small pox disease.
Vaccination of 23 persons:
Later, Jenner used material from cowpox lesions for vaccination and successfully vaccinated 23
persons. As the material he used was obtained from cow (latin vacca), the term vaccination was
used by Louis Pasteur for inoculation against disease.
First disease to have effective method of prevention: The first infectious disease against
which effective method of prevention was developed was a viral disease.
One of Pasteur‟s associates, Charles Chamberland, found that bacteria can not pass through
porcelain filters, while agent responsible for rabies (a disease which is transferred to human by
bites of rabid dogs, foxes, cats, bats and other animals) can pass through these filters. As in those
days the word virus was loosely used to describe any toxic substance that caused disease, those
unseen filterable agents of disease were described as filterable viruses.
Ivanowski (1892):
In 1892, Ivanowski discovered that the agent which caused tobacco mosaic disease was filterable.
He obtained filtrate free of bacteria from ground up leaves of infected plants and placed it on
healthy leaves of tobacco. He observed that filtrate produced the disease in healthy plants.
Presence of similar filter-passing, ultramicroscopic agents was seen in the victims of many diseases,
including foot and mouth disease (1898) and yellow fever (1901).
Twort (1915):
Twort in 1915 and D‟Herelle in 1917 independently discovered bacteriophages, viruses that infect
bacteria. Twort observed that bacteria colonies sometimes undergo Lysis (dissolved and
disappeared) and that this lysis can be transferred from one colony to other. Even highly dilute
material from lysed colony can transfer the lytic effect.
However, heating the filtrate destroyed its lytic property. From these observations he
concluded that lytic agent might be a virus.
D’Herelle (1917):
D‟Herelle rediscovered the same phenomenon as Twort did and used the word bacteriophages
meaning “bacteria eater”.
Stanley (1935):
The filterable agents were first purified in 1935, when Stanley was successful in crystallizing
the tobacco mosaic virus. Chemical analysis of these particles showed that they contained
only nucleic acid and protein. This suggested that, unlike other forms, viruses are of simple
chemical composition.
CHARACTERISTICS OF VIRUSES:
SIZE:
Viruses are extremely small infectious agents, which can only be seen under an electron
microscope. They range in size from 250 nanometer (nm) of poxviruses to the 20 nm of
parvoviruses.
FILTERABLE:
Viruses are 10 to 1000 times smaller than most bacteria, so they can pass through the pores of
filter, from which bacteria cannot pass.
Viruses cannot be grown on artificial media. They can reproduce only in animal and plant cells or
in microorganisms. They reproduce by replication (a process by which many identical copies of
virus are formed). Thus the viruses are obligate intracellular parasites.
Viruses lack metabolic machinery for the synthesis of their own nucleic acid and protein. They
depend on the host cell to carry out these vital functions.
During reproduction in the host cells, viruses may cause disease. All viruses are generally resistant
to broad range of available antibiotics such as penicillin, streptomycin and others.
STRUCTURE OF VIRUSES:
NUCLEOCAPSID: The complete, mature and infectious particle is known as virion. The virions
are composed of a central core of nucleic acid, either DNA or RNA, which is also known as the
genome and is surrounded by a protein coat, the capsid. Capsid gives definite shape to virion.
CAPSOMERES:
Example:
ENVELOPE:
In some animal viruses the nucleocapsid (nucleic acid and capsid) is covered by another
membrane which is derived from the host cell. This covering of nucleocapsid is known as the
envelope. Non enveloped viruses are known as naked virions.
SHAPES:
SHAPES OF BACTERIOPHAGES:
Cubical symmetry:
In general appearance cubical phages are regular solid or icosahedral having 20 faces.
Helical symmetry:
Helical phages are rod shaped
Many phages consist of head and tail. In those cases heads are polyhedral but tails are rod
shaped.
Prions are least understood microorganisms which are infectious proteins. They were most recently
discovered in 1983.
Composition:
Their nature is very controversial. They are composed of protein only that contains the information
that codes for their own replication. All other organisms contain their genetic information in
nucleic acid (DNA or RNA).
Diseases:
Prions are responsible for “mad cow infection” and “mysterious brain infection in man”.
BACTERIOPHAGES:
The viruses which infect bacteria such as Escherichia coli are called bacteriophages.
T-PHAGES:
Bacteriophages are also simply known as phages. Earlier researches on bacteriphages were mainly
on limited number of phages that infect these bacteria. Of these the best known phages are T
phages (T for type).
Among T phages, the T2 and T4 phages are mainly used in phage studies.
STRUCTURE OF T-PHAGE:
The overall structure of T4, studied with electron microscopy, resembles that of tadpole consisting
of following parts:
Head:
The head is an elongated pyramidal (having two triangular structures with common base),
hexagonal, prism -shaped structure, to which straight tail is attached. Within the head double
stranded DNA molecule is present.
TAIL:
The structure of phage tail is more complex than head. It consists of following parts:
Tail sheath: The outer most tube of tail present between head and tail fibers is called tail
sheath.
Collar: At one end, the tail is connected with head through a structure called collar.
End plate: At other end, the tail is connected with tail fibers with a structure called end
plate.
Tail Core: A tube of distinct protein which is enclosed by tail sheath is called tail core.
Tail fibers: Tail fibers are the structures which are attached with end plate. are attached,
which are the structures for attachment. The volume of the phage is about
1/1000 of the host.
(A bacteriophage)
The bacteriophage replicates only inside the bacterial cell. The different steps of the life cycle are
as:
LYTIC CYCLE:
Attachment (adsorption) Phage attaches at receptor site on the cell wall of bacterium. During
attachment, week chemical union between virion and receptor site takes place.
Penetration:
The tail of bacteriophage releases the enzyme called lysozyme to dissolve a portion of the
bacterial cell wall. The tail sheath contracts and tail core is forced into the cell through cell wall and
cell membrane. The virus injects its DNA into the cell same as the syringe is used to inject the
vaccine.
The protein coat, which forms the phage head and tail structure of virus remains outside the cell.
Many animal viruses, however enter the host cell as a whole.
Multiplication:
Immediately after entering the host cell , the viral nucleic acid takes the control of the host‟s
biosynthetic machinery and induces the host cell to synthesize necessary viral components (dna,
proteins), and starts multiplying.
Lysis:
About 25 minutes after initial infection, approximately 200 new bacteriophages are formed inside
the bacterial cell. The result is bacterial cell bursts, i.e. it undergoes lysis. Newly formed phages are
released to infect the other bacteria and start lytic cycle.
Lytic phage:
The phage which causes lysis of the host cell is known as lytic or virulent phage. All infections of
bacterial cells by phages do not result in lysis.
LYSOGENIC CYCLE:
Lysogeny:
In some cases when the viral DNA is penetrated into bacterium, it becomes incorporated into the
bacterial chromosome instead of taking over the control of host‟s machinery. Phage in this state is
called prophage and this process is known as lysogeny.
During lysogeny, the bacterium continues to live and reproduce normally. Viral DNA being the part
of bacterial chromosome passes to each daughter cell in all successive generations.
Resistant to Infection:
Temperate phage: The phage which causes lysogeny is called temperate (lysogenic) phage.
Induction:
Some times, the viral DNA gets detached from the host‟s chromosome and lytic cycle starts. This
process is called induction.
(Replication of Bacteriophages)
CLASSIFICATION OF VIRUSES:
On the basis of morphology viruses are classified into rod shaped (T.M.V), spherical (poliovirus)
and tadpole like bacteriophage viruses etc.
Nucleic acid properties are most important for classifying plant, animal and
bacterial viruses. The viruses may be DNA or RNA on the basis of genetic material.
If the viruses are without covering of their nucleocapsids, these are called naked viruses. If these
are with outer covering called enveloped or complex virues.
There are many diseases which are caused by viruses. The diseases which have been or
common in Pakistan are as follows:
SMALL POX:
Causative agent: Smallpox is caused by pox viruses (the DNA enveloped virus).
History: It is an ancient disease that is known to have occurred as epidemic in China as early as
the twelfth century B.C. Until the early twentieth century, small pox was a common disease
throughout the world.
Symptoms: In small pox, raised fluid-filled vesicles are formed on the body which later on
become pustules and form pitted scars. These pitted scars are also called the pocks.
Control: By 1950‟s, immunization and other control measures had largely decreased the danger,
but it is still present in the third world countries where many people are affected. In 1980, it was
declared by World Health Organization that small pox has been eradicated from the world.
HERPES SIMPLEX:
Symptoms: In this disease, vascular lesions in the epithelial layers of ectodermal tissues are
formed.
Infected body areas: Most commonly this disease occurs in the mouth, on the lips, and at other
skin sites.
INFLUENZA:
Spread: Influenza is wide spread disease in man and occurs in epidemic form.
Causative agent:
It is caused by Mumps and Measles viruses which belong to group paramyxoviruses. They are large,
enveloped, RNA viruses.
Spread:
Mumps is highly contagious, wide spread, but seldom fatal. Measles is one of the commonest
diseases of the childhood and adult human population is equally susceptible the world over.
Control: About 60% of adults are immune to it. This disease develops immunity in its victim.
POLIO:
Causative agent: Poliomyelitism is caused by polio virus. The polioviruses are the smallest
known viruses and contain RNA in spherical capsid.
Spread: It is found all over the world. It occurs mostly in childhood. The age at which primary
infection occurs varies with social and economic factors.
RETROVIRUSES:
The viruses which are associated with tumor production in a number of animal species, such as
fowl, rodents and cats, are known as retroviruses. RNA tumor viruses have been known for many
years. These viruses are widely distributed in nature.
EXAMPLE: The most familiar of retroviruses is the human immunodeficiency virus (HIV)
which causes acquired immune deficiency syndrome (AIDS).
The single stranded RNA tumor viruses, which also include retroviruses (oncoviruses), are
spherical in form, about 100nm in diameter and enveloped by host plasma membrane.
NON-SPECIFIC: A few retroviruses are non-specific that is they can infect any cell.
HOST SPECIFIC: Most of them can infect only host cells that possess required receptors.
Example:
In the case of AIDS virus, the host cell possesses a receptor that allows the viral adsorption and
penetration in several types of leukocytes (white blood cells) and tissue cells.
REVERSE TRANSCRIPTASE:
The retroviruses have a special enzyme called reverse transcriptase, which can convert a single -
stranded RNA genome into double stranded viral DNA.
PROVIRUS:
The DNA of retrovirus after infection in host cells when becomes incorporated into its genome, it is
called as a provirus. Provirus can can be passed on to daughter cells. In this way, some of
retroviruses can convert normal cells into cancer cells.
DISCOVERY IN HOMOSEXUALS:
The AIDS was reported by some physicians in young patients with homosexual activity early in
1980‟s. They had one or more of complex symptoms such as:
severe pneumonia
a rare vascular cancer
DISCOVERY IN NON-HOMOSEXUALS:
Soon after the disease was discovered in non-homosexual patients who were given blood (blood
transfusion) or blood products.
CAUSATIVE AGENT:
INFECTION OF T-LYMPHOCYTES:
The major cell infected by HIV is the helper T-lymphocyte, which is major component of immune
system. As the HIV infection continues in the host, the decrease of helper T-lymphocytes results in
failure of the immune system and the infected person becomes susceptible to other diseases. Cells
in central nervous system can also be infected by HIV.
HOST SPECIFIC:
Recent studies on HIV reveal that the virus infects and multiplies in monkey but does not cause
disease in them, which means that HIV is host specific.
TRANSMISSION:
The HIV is transmitted by intimate sexual contact, contact with blood and breast feeding.
Healthcare workers can also acquire HIV during professional activities.
CONTROL:
Avoiding the direct contact with HIV is important measure for preventing the disease. Prevention
of‟ intravenous drugs with common syringes and use of sterile needles/syringes and utensils is
important.
VACCINATION:
Now vaccine against HIV has been synthesized and its experimental administration in humans
started in early 2001 in South Africa.
HEPATITIS:
Hepatitis is an inflammation of the liver. It is usually caused by viral infection, toxic agents or
drugs.
It is characterized by jaundice, abdominal pain, liver enlargement, fatigue and sometimes fever. It
may be mild or can be acute and can lead to liver cancer.
TYPES OF HEPATITIS:
The different types of viral hepatitis are: Viruses of hepatitis A, B and C are better known.
Causative agents: Hepatitis A virus (HAV) is an RNA virus (non enveloped). It causes mild
short term, less virulent disease.
Causative agent:
Hepatitis B (HBV) is the second major form of hepatitis. It is caused by DNA virus which is very
common in Asia, China, Philippines, Africa and the Middle East.
Transmission:
Hepatitis B is transmitted by the exchange of body fluids, for example blood serum, breast milk and
saliva, from mother to child during birth or afterward and by sexual contact.
Symptoms:
During acute attacks of Hepatitis B, fatigue, loss of appetite and jaundice are reported.
Treatment:
Infected persons can recover completely and become immune to the virus. Genetically engineered
vaccine is available for HBV.
Causative agent:
It is caused by Hepatitis C virus (HCV) which is RNA enveloped virus. It causes infusion hepatitis,
which is less severe than hepatitis A or hepatitis B.
Transmission:
It is transmitted through blood transfusion, from mother to child during and after pregnancy and
by sexual contact. Hepatitis C often leads to chronic liver disease.
HEPATITIS E
Transmission:
Its virus is transmitted through the feces of an infected person. Most recent work of Halbur and
coworker (2001) reveals that pig could be the source of infection of hepatitis E.
HEPATITIS F & G
People with chronic hepatitis infection are at the risk of liver damage. Hepatitis can be controlled
by adopting hygienic measures, with routine vaccination and screening of blood/ organ/ tissue of
the donor.