B.Sc.

II year (Biotechnology), NEP [ Session - 2024-25]

Paper I (Major)
Month Class Paper Topics to be covered No. of
expected
lectures

September B.Sc II Paper I – Basic Unit 1 20 theory

Year Molecular 1.1 Genome organization: 04 practicals
Biology
Anatomy of gene, gene structure of
prokaryotes and eukaryotes- Eukaryotic
nuclear genomes, Packaging of DNA into
chromosomes, Special features of
metaphase chromosomes, Families of
genes. The repetitive DNA content of
Genomes- Tandemly repeated DNA.
Satellite DNA.

Minisatellites and microsatellite. Flow of

genetic information- Central Dogma-
DNA replication, transcription and
translation. Gene Regulation in
Prokaryotes and Eukaryotes.

1.2 Cell signaling:

Modes of Cell Signaling- Exocrine,

endocrine, Autocrine, Paracrine,
Juxtacrine. Pathways of cell signaling
with examples. Hormones and their
receptors, secondary messengers,
signaling through G protein coupled
receptors.

Enzyme-linked receptors, Cell surface

receptors, Intra cellular receptors.

1.3 Cancer:

Causes of Cancer

Types of Cancer: Begnin and Malignant

Development of Cancer.

Oncogenes, Tumor suppressor genes,

Cancer and the cell cycle, Necrosis.

And Practicals.
Month Class Paper Topics to be covered No. of
expected
lectures
October B.Sc II Paper I – Basic Unit 2: 18 theory
Year Molecular 2.1 Replication: 04 practicals
Biology
Prokaryotic and Eukaryotic replication:
models for replication, Unit of
replication, replication initiation,
elongation and termination, replication
inhibitors.

2.2 DNA repair:

Direct reversal, Excision repair -

nucleotide and base excision, Mismatch
repair Trans lesion DNA synthesis,
Recombination repair, SOS Response.

2.4 DNA recombination:

Models for recombination, Enzymes and

proteins involved in recombination, Site-
specific recombination.

And Practicals.
November B.Sc II Paper I – Basic Unit 3: 22 theory
Year Molecular 05 practicals
Biology Transcription: Prokaryotic and Eukaryotic
transcription: Typical Features-
Transcription factors, Initiation,
Elongation, Termination.

RNA polymerases, Types of RNA

polymerases- Prokaryotic and
Eukaryotic.

General and specific transcription

factors, Promoters, insulator, repressor,
enhancer.

And Practicals.
 Month Class Paper Topics to be covered No. of
expected
lectures
December B.Sc II Paper I – Basic Unit 4: 22 theory
Year Molecular Translation: Prokaryotic and eukaryotic 04 practicals
Biology translation: Translation machinery,
initiation, elongation and termination
factors, translational inhibitors.

Regulation of translation.

And Practicals.
January B.Sc II Paper I – Basic Unit 5: 22 theory
Year Molecular 05 practicals
Biology 5.1 Control of gene expression in
Prokaryotes: DNA binding proteins,

posttranscriptional control of gene

expression. Gene regulation in Bacteria,
Gene silencing.

Overview of ribozyme technology

5.2 Control of gene expression in

Eukaryotes: enhancers, chromatin
remodeling.

5.3 Mutation:

Types and causes, mutant types-lethal,

conditional, biochemical, loss of
function, gain of function

Mutation:- Types of mutations- Point

mutation (Base pair change, frame shift,
deletion). Structural changes- Deletion,
Duplication, inversion and translocation
and numerical changes ploidy.

And Practicals.

Dr. Prabhat Kumar Saxena Head of the Department

 B.Sc I Year (Biotechnology) NEP – Session 2023 – 2024
Paper I (Major)
Month Class Paper Topics to be covered No. of
expected
lectures
September B.Sc I Paper I –Cell Unit 1: 22 theory
Year Biology and 04 practicals
Biochemistry Cell as a Basic Unit:

1. Historical background of the Cell

1.1 History of Cell Biology.
1.2 Cell Structure.
1.3 Cell Theory.
2. Prokaryotic Cell and Cell Organells:
2.1 Ultrastructure of Prokaryotic Cell.
2.2.Structure and function of cell
organelles: Cytoplasmic membrane,
Nuclear region,
Metachromatic granules, Spores and
Microfilaments, Centriole. Flagella. Pili,
Cell, wall.
Ribosomes, Vacuoles. Cysts,
Microtubules.

2.3 Difference between Prokaryotic

and Eukaryotic cells.

And Practicals.
October B.Sc I Paper I –Cell Unit 2: 20 theory
Year Biology and 05 practicals
Biochemistry Cell Organelles and Cell Cycle:
1. Eukaryotic Cell and Cell Organells:
1.1 Ultratructure of Eukaryotic cell
(Plant and Animal cells).
1.2 Structure and function of cell
organelles: Cell membrane, Mitochondria,
Chloroplast, Endoplasmic reticulum,
Golgi bodies. Lysosomes. Peroxisomes,
Nucleus
2. Cell Cycle:
2.1 Cell cycle and Cell division.
2.2 Apoptosis or Cell death

And Practicals.
November B.Sc I Paper I –Cell Unit 3: 22 theory
Year Biology and Molecular Structure of Water: 04 practicals
Biochemistry 1. Water structure and Buffer:
1.1 Properties of Water.
1.2 Interaction of Water.
1.3 Role of Water in Bio molecular
Structure
1.4 Acid and Bases, Buffer solutions.
2. Chemical Bonds:
2.1 Chemical Bonds (lonic Bond, Covalent
Bond. Coordinate Bond, Non
Covalent Bonds, Hydrogen Bond)

And Practicals.
December B.Sc I Paper I –Cell Unit 4: 18 theory
Year Biology and Biomolecules: 04 practicals
Biochemistry Sources, Nomenclature, Classification,
Structures. Characteristics, and Functions:
Carbohydrates,
2.Lipids.
3. Proteins and Nucleic Acids.

And Practicals.
January B.Sc I Paper I –Cell Unit 5: 22 theory
Year Biology and 05 practicals
Biochemistry Tools and Techniques
1.1 Principle and Applications of Light
Microscopy. Centrifugation. Chromatography
(Paper. Thin layer and Column). Colorimeter
and Spectrophotometer.

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

B.Sc I Year (Biotechnology) NEP – Session 2024 – 2025

Paper I (Major)
Month Class Paper Topics to be covered No. of
expected
lectures
July B.Sc I Paper I –Cell Unit 1: 22 theory
Year Biology and Cell as a Basic Unit: 04 practicals
Biochemistry 1. Historical background of the Cell
1.1 History of Cell Biology.
1.2 Cell Structure.
1.3 Cell Theory.
2. Prokaryotic Cell and Cell Organells:
2.1 Ultrastructure of Prokaryotic Cell.
2.2.Structure and function of cell
organelles: Cytoplasmic membrane,
Nuclear region,
Metachromatic granules, Spores and
Microfilaments, Centriole. Flagella. Pili,
Cell, wall.
Ribosomes, Vacuoles. Cysts,
Microtubules.
2.3 Difference between Prokaryotic
and Eukaryotic cells.

And Practicals.
August B.Sc I Paper I –Cell Unit 2: 20 theory
Year Biology and 05 practicals
Biochemistry Cell Organelles and Cell Cycle:
1. Eukaryotic Cell and Cell Organells:
1.1 Ultratructure of Eukaryotic cell
(Plant and Animal cells).
1.2 Structure and function of cell
organelles: Cell membrane, Mitochondria,
Chloroplast, Endoplasmic reticulum,
Golgi bodies. Lysosomes. Peroxisomes,
Nucleus,
2. Cell Cycle:
2.1 Cell cycle and Cell division.
2.2 Apoptosis or Cell death

And Practicals.
September B.Sc I Paper I –Cell Unit 3: 22 theory
Year Biology and Molecular Structure of Water: 04 practicals
Biochemistry 1. Water structure and Buffer:
1.1 Properties of Water.
1.2 Interaction of Water.
1.3 Role of Water in Bio molecular
Structure
1.4 Acid and Bases, Buffer solutions.
2. Chemical Bonds:
2.1 Chemical Bonds (lonic Bond, Covalent
Bond. Coordinate Bond, Non
Covalent Bonds, Hydrogen Bond)

And Practicals.
October B.Sc I Paper I –Cell Unit 4: 18 theory
Year Biology and Biomolecules: 04 practicals
Biochemistry Sources, Nomenclature, Classification,
Structures. Characteristics, and Functions:
Carbohydrates,
2.Lipids.
3. Proteins and Nucleic Acids.

And Practicals.
November B.Sc I Paper I –Cell Unit 5: 22 theory
Year Biology and 05 practicals
Biochemistry Tools and Techniques
1.1 Principle and Applications of Light
Microscopy. Centrifugation. Chromatography
(Paper. Thin layer and Column). Colorimeter
and Spectrophotometer.

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. Semester-1 [Session 2024 – 2025]

Paper 3 (Molecular Endocrinology and Reproductive Technology)
Month Class Paper Topics to be covered No. of
expected
lectures
July M.Sc Paper 3 – Unit 1: 22 theory
I Sem Molecular 1. Definition and scope of 04 practicals
Endocrinology molecular Endocrinology.
and 2. Chemical nature of Hormones.
Reproductive 3. Purification and
Technology characterization of Hormones.
4. Production of Hormones by
DNA technology.

And Practicals.
August M.Sc Paper 3 – Unit 2: 20 theory
I Sem Molecular 04 practicals
Endocrinology 1. Hormones receptors-
and Identification, quantitation,
Reproductive purification, and physico
Technology 2. chemical properties.
3. Membrane receptors Structure
and signal transudation
Mechanism. Nuclear receptors
-Stricter and function, orphan
receptors.
4. Neurohormantes as neural
messengers.

And Practicals.
September M.Sc Paper 3 – Unit 3: 20 theory
I Sem Molecular 05 practicals
Endocrinology 1. Eicosanoids and Hormones
and action
Reproductive 2. Molecular mechanism of
Technology prostaglandin metabolism.
Hormonal regulation of energy
metabolism.
 3. Genetic analysis of hormonal
disorders.

And Practicals.
Month Class Paper Topics to be covered No. of
expected
lectures
October M.Sc I Paper 3 – Unit 4: 18 theory
Sem Molecular 04 practicals
Endocrinology 1. Contraception
and 2. Multiple ovulation and
Reproductive embryo transfer technology.
Technology 3. Study of estrus cycle by
vaginal smear technique.
4. Surgical technique-
castration, ovaneciomy,
vasectomy, tubectomy and
laprotomy.

And Practicals.
November M.Sc I Paper 3 – Unit 5: 21 theory
Sem Molecular 05 practicals
Endocrinology 1. Sex determination.
and 2. Embryo sexing and cloning
Reproductive 3. Hormonts and againg
Technology 4. Transcriptional and post
Transcriptiorial
regulation of Hormones

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. I Semester [Session 2023 – 2024]

Paper 3 (Molecular Endocrinology and Reproductive Technology)
Month Class Paper Topics to be covered No. of
expected
lectures
September M.Sc Paper 3 – Unit 1: 22 theory
I Sem Molecular 1. Definition and scope of 04 practicals
Endocrinology molecular Endocrinology.
and 2. Chemical nature of Hormones.
Reproductive 3. Purification and characterization
Technology of Hormones.
4. Production of Hormones by DNA
technology.

And Practicals.
October M.Sc Paper 3 – Unit 2: 20 theory
I Sem Molecular 04 practicals
Endocrinology 1. Hormones receptors-
and Identification, quantitation,
Reproductive purification, and physico
Technology 2. chemical properties.
3. Membrane receptors Structure
and signal transudation
Mechanism. Nuclear receptors -
Stricter and function, orphan
receptors.
4. Neurohormantes as neural
messengers.

And Practicals.
November M.Sc Paper 3 – Unit 3: 20 theory
I Sem Molecular 05 practicals
Endocrinology 1. Eicosanoids and Hormones
and action
Reproductive 2. Molecular mechanism of
Technology prostaglandin metabolism.
Hormonal regulation of energy
metabolism.
3. Genetic analysis of hormonal
disorders.
 And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
December M.Sc I Paper 3 – Unit 4: 22 theory
Sem Molecular 05 practicals
Endocrinology 1. Contraception
and 2. Multiple ovulation and
Reproductive embryo transfer technology.
Technology 3. Study of estrus cycle by
vaginal smear technique.
4. Surgical technique-castration,
ovaneciomy, vasectomy,
tubectomy and laprotomy.

Unit 5:

1. Sex determination.
2. Embryo sexing and cloning
3. Hormonts and againg
4. Transcriptional and post
Transcriptiorial
regulation of Hormones

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. Semester-1 [Session 2024 – 2025]

Paper 4 (BIOINSTRUMENTATION)
Month Class Paper Topics to be covered No. of
expected
lectures
January M.Sc Paper 4 – Unit 1: 22 theory
II BIOINSTRUMENTATION 1. Centrifugation: Principal, 04 practicals
Sem techniques, preparative
ulimscentrifugation and
analytical ultracentrifugation,
Sedimentation coefficient and
factors affecting sedimentation
coefficient.
2. Application of Centrifugation.
3. Photometry: Basic principal of
colonmeiry, UV- visible
spectrophotometry and IR
spectrophotometry
4. Atomic absorption
spectroscopy. Principles,
instrumentation and
applications.

And Practicals.
February M.Sc Paper 4 – Unit 2: 20 theory
II BIOINSTRUMENTATION 04 practicals
Sem 1. Theory, Principle and
applications of paper and TLC
Gel filtration and lon exchange
chromatography
2. Affinity chromatography and
HPCL
3. Gas-liquid chromatography

And Practicals.
March M.Sc Paper 4 – Unit 3: 22 theory
II BIOINSTRUMENTATION 05 practicals
Sem 1. Electrophoresis Principal and
Theory
2. Paper electrophoresis, starch
gel, agarose, PAGE-types, 2D-E
Isoelectricfocussing,
Immunodiffusion,
Immunoelectrophoresis.
 3. Southem, Northern and
Western Blotting

And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
April M.Sc II Paper 4 – Unit 4: 22 theory
Sem BIOINSTRUMENTATION 04 practicals
1. Fluorescence spectroscopy.
Principals, mistrumentation
and applications
2. Nephlomery & Turbidometry
NMR, Principles,
instrumentahon and
applications
3. X ray crystallography
Principles, marumentation
and applications

And Practicals.
May M.Sc II Paper 4 – Unit 5: 20 theory
Sem BIOINSTRUMENTATION 04 practicals
1. Microtomy, types, principals
and applications
2. Microscopy Light, Phase
comuras, fubrescence and
electron microscopies
Radioactivity Liquid.
Scinullation Counter, solsd
Scinnflation counters.g RIA
and Autoradiography:
Principles and applications

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. Semester-1 [Session 2023 – 2024]

Paper 4 (BIOINSTRUMENTATION)
Month Class Paper Topics to be covered No. of
expected
lectures
January M.Sc Paper 4 – Unit 1: 22 theory
II BIOINSTRUMENTATION 1. Centrifugation: Principal, 04 practicals
Sem techniques, preparative
ulimscentrifugation and
analytical ultracentrifugation,
Sedimentation coefficient and
factors affecting sedimentation
coefficient.
2. Application of Centrifugation.
3. Photometry: Basic principal of
colonmeiry, UV- visible
spectrophotometry and IR
spectrophotometry
4. Atomic absorption
spectroscopy. Principles,
instrumentation and
applications.

And Practicals.
February M.Sc Paper 4 – Unit 2: 20 theory
II BIOINSTRUMENTATION 04 practicals
Sem 1. Theory, Principle and
applications of paper and TLC
Gel filtration and lon exchange
chromatography
2. Affinity chromatography and
HPCL
3. Gas-liquid chromatography

And Practicals.
March M.Sc Paper 4 – Unit 3: 22 theory
II BIOINSTRUMENTATION 05 practicals
Sem 1. Electrophoresis Principal and
Theory
2. Paper electrophoresis, starch
gel, agarose, PAGE-types, 2D-E
Isoelectricfocussing,
Immunodiffusion,
Immunoelectrophoresis.
 3. Southem, Northern and
Western Blotting

And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
April M.Sc II Paper 4 – Unit 4: 22 theory
Sem BIOINSTRUMENTATION 04 practicals
1. Fluorescence spectroscopy.
Principals, mistrumentation
and applications
2. Nephlomery & Turbidometry
NMR, Principles,
instrumentahon and
applications
3. X ray crystallography
Principles, marumentation
and applications

And Practicals.
May M.Sc II Paper 4 – Unit 5: 20 theory
Sem BIOINSTRUMENTATION 04 practicals
1. Microtomy, types, principals
and applications
2. Microscopy Light, Phase
comuras, fubrescence and
electron microscopies
Radioactivity Liquid.
Scinullation Counter, solsd
Scinnflation counters.g RIA
and Autoradiography:
Principles and applications

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. Semester-3 [Session 2024 – 2025]

Paper 2 (Bioprocess Engineering and Bioinformatics)
Month Class Paper Topics to be covered No. of
expected
lectures
July M.Sc Paper 2 – Bioprocess Unit 1: 22 theory
III Engineering and 1. Isolation, screening and 04 practicals
Sem Bioinformatics maintenance of industrially
important microbes
2. Strain improvement for
increased yield and other
desirable characteristics
3. Bioreactor design: types of
fermentation and fermenters
4. Fermentation media, large scale
animal and plant cell cultivation
And Practicals.
August M.Sc Paper 2 – Bioprocess Unit 2: 20 theory
III Engineering and 1. Filtration, centrifugation, 04 practicals
Sem Bioinformatics sedimentation and flocculation
method of biosepration of
fermentation products
2. Drying, crystallization, storage
and packaging of fermentation
products
3. Enzymatic bioconversions e g.
starch and sugar conversion
process.
4. Cheese making by proteases
and various other enzyme
catalytic action in food
processing
And Practicals.
September M.Sc Paper 2 – Bioprocess Unit 3: 22 theory
III Engineering and 1. Food ingredients and additives 05 practicals
Sem Bioinformatics prepared by fermentation and
their purification
2. Microbes and their use in
pickling, produong colors and
flavors.
3. Process wastes-whey, molasses,
starch substrates and other food
wastes for bioconversion to
useful products
4. Bacteriocins from lactic acid
bacteria, its production and
 applications in food
preservation
And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
October M.Sc III Paper 2 – Bioprocess Unit 4: 18 theory
Sem Engineering and 04 practicals
Bioinformatics 1. Proteins and nucleic acid
databases
2. The NCBI publicly available
tools
3. The gene bank sequences to
the databases, submitting
ONA sequences to the
databases and database
searching
4. Computational tools for DNA
sequence analysis

And Practicals.
November M.Sc III Paper 2 – Bioprocess Unit 5: 22 theory
Sem Engineering and 04 practicals
Bioinformatics 1. Sequence alignment, pair
wise alignment techniques,
multiple sequence alignment
2. Use of CLUSTAL for the
multiple sequence augment
3. Submitting ONA protein
sequences 10 databases
4. Submitting aligned set of
sequences updates and
internet resources

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

M.Sc. Semester-3 [Session 2023 – 2024]

Paper 2 (Bioprocess Engineering and Bioinformatics)
Month Class Paper Topics to be covered No. of
expected
lectures
September M.Sc Paper 2 – Bioprocess Unit 1: 22 theory
III Engineering and 1. Isolation, screening and 04 practicals
Sem Bioinformatics maintenance of industrially
important microbes
2. Strain improvement for
increased yield and other
desirable characteristics
3. Bioreactor design: types of
fermentation and fermenters
4. Fermentation media, large scale
animal and plant cell cultivation
And Practicals.
October M.Sc Paper 2 – Bioprocess Unit 2: 20 theory
III Engineering and 1. Filtration, centrifugation, 04 practicals
Sem Bioinformatics sedimentation and flocculation
method of biosepration of
fermentation products
2. Drying, crystallization, storage
and packaging of fermentation
products
3. Enzymatic bioconversions e g.
starch and sugar conversion
process.
4. Cheese making by proteases
and various other enzyme
catalytic action in food
processing
And Practicals.
November M.Sc Paper 2 – Bioprocess Unit 3: 22 theory
III Engineering and 1. Food ingredients and additives 05 practicals
Sem Bioinformatics prepared by fermentation and
their purification
2. Microbes and their use in
pickling, produong colors and
flavors.
3. Process wastes-whey, molasses,
starch substrates and other food
wastes for bioconversion to
useful products
4. Bacteriocins from lactic acid
bacteria, its production and
applications in food
preservation
 And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
December M.Sc III Paper 2 – Bioprocess Unit 4: 23 theory
Sem Engineering and 04 practicals
Bioinformatics 1. Proteins and nucleic acid
databases
2. The NCBI publicly available
tools
3. The gene bank sequences to
the databases, submitting
ONA sequences to the
databases and database
searching
4. Computational tools for DNA
sequence analysis

Unit 5:

1. Sequence alignment, pair

wise alignment techniques,
multiple sequence alignment
2. Use of CLUSTAL for the
multiple sequence augment
3. Submitting ONA protein
sequences 10 databases
4. Submitting aligned set of
sequences updates and
internet resources

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

B.Sc. I Year (Biotechnology) NEP – Session 2023 – 2024

Paper II (Major/Minor/Open Elective)
Month Class Paper Topics to be covered No. of
expected
lectures
September B.Sc. I Paper II – Unit 1: 20 theory
Year Microbiology History, Basic concepts of Microbiology and 04 practicals
and Culture Media preparation
Immunology
1. History, Basic concepts of Microbiology:

1.1 Fundamental, History and evolution of

microbiology, Development of microbiology.
Application of microbiology in human welfare.

1.2 Classification, General characteristic and

structure of Bacteria, Fungi and Viruses.

2. Media Preparation:

2.1 Methods and Types: Culture, Minimal,

Selective, differential. Transport media.

2.2 Synchronous, Batch and Continuous culture.

And Practicals.
October B.Sc. I Paper II – Unit 2: 22 theory
Year Microbiology 05 practicals
and Microbial Growth and Growth measurement:
Immunology 1. Microbial Growth:

1.1. Definition of growth, Mathematical

expression of growth Growth Curve, Generation
time. Growth yield, Effect of nutrients on
growth.

1.2. Factor affecting growth: Nutrient,

Temperature, Oxygen, pH, Osmotic pressure.

2. Growth measurement:

2.1 Measurement of Growth (Direct and Indirect

methods): cell number, Cell Mass and Cell
Activity.

2.2. Cell Count: Turbidometric method, Plate

count method. Membrane count method, Dry
weight and Wet method by measurement of
cellular activity.
 And Practicals.

Month Class Paper Topics to be covered No. of

expected
lectures
November B.Sc I Paper II – Unit 3: 22 theory
Year Microbiology Basics of Immunology: 05 practicals
and 1. Basics of Immunology:
Immunology
11. Concept of Innate and Acquired immunity,
Phygocytosis complement and Inflammatory
responses.

1.2. Immune cells and organs: Structure. Function

and Properties of immune cells - Stem cell, T-cell,
B-cell, NK-cell. Macrophagus, Neutrophil,
Eosinophil, Basophil, Mastcell, Dentric cell.

1.3. Immune organ: Bone marrow, Thymus,

Lymph Node. Spleen, Lymphatic System.

And Practicals.
December B.Sc I Paper II – Unit 4: 20 theory
Year Microbiology 04 practicals
and Immunoglobulins and Immune response:
Immunology
1. Immunoglobulins

1.1. Antigens: Characteristics of an antigen:

Foreignness. Molecular size, Chemical
composition and Heterogeneity, Antigen
Adjuvants, Epitopes, Haptens.

1.2. Antibodies: Structure, Types, Functions and

Properties of antibodies Antigenic determinant
on antibodies (Isotypic. Allotypic, Idiotypic).
Monoclonal, Polyclonal and Chimeric antibody.

2.Immune response:

2.1. Generation of immune response: Primary

and Secondary immune response, generation of
Humoral response (Plasma and Memory cell).

Generation of cell mediated immune response

(self MHC restriction, T-cell activation, Co-
 stimulatory signals). Killing Mechanisms by CTL
and NK cells. Introduction to tolerance.
And Practicals.
January B.Sc I Paper II – Unit 5: 16 theory
Year Microbiology Microbial, Immunological Techniques and 04 practicals
and Vaccination:
Immunology
1. Microbial Techniques :

1.1. Principle, Working and applications of

instruments -Laminar airflow, Autoclave, Hot air
oven.

2. Immunological techniques:

2.1. RIA, ELISA, Western blotting. Principles of

Precipitation. Agglutination, Immunodiffsion,
Immunoelectophorosis.

3. Vaccination:

3.1. Vaccines and vaccination: Rubella, Varicella

(Chickenpox), Polio, Diptheria. Hepatitis vaccine.

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department

B.Sc I Year - Biotechnology– Session 2023 – 2024

I paper (Major - I)
Month Class Paper Topics to be covered No. of
expected
lectures
July B.Sc I Paper I –Cell Unit 1: History, Basic concepts of 20 theory
Year Biology and Microbiology and Culture Media preparation 04 practicals
Biochemistry 1. History, Basic concepts of Microbiology:
1.1 Fundamental, History and evolution of
microbiology. Development of microbiology.
Application of microbiology in human welfare.
1.2 Classification, General characteristic and
structure of Bacteria, Fungi and Viruses.
2. Media Preparation:
2.1 Methods and Types: Culture, Minimal,
Selective, differential. Transport media.
2.2 Synchronous. Batch and Continuous culture.

And Practicals.
August B.Sc I Paper I –Cell Unit 2: Microbial Growth and Growth 22 theory
Year Biology and measurement: 05 practicals
Biochemistry 1. Microbial Growth:
1.1. Definition of growth. Mathematical expression
of growth Growth Curve, Generation time. Growth
yield. Effect of nutrients on growth.
1.2. Factor affecting growth: Nutrient,
Temperature. Oxygen, pH, Osmotic pressure.
2. Growth measurement:
2.1 Measurement of Growth (Direct and Indirect
methods) cell number, Cell Mass and Cell Activity.
2.2. Cell Count: Turbidometric method. Plate count
method. Membrane count method, Dry weight
and Wet method by measurement of
cellular activity
And Practicals.
September B.Sc I Paper I –Cell Unit 3: Basics of Immunology: 22 theory
Year Biology and 1. Basics of Immunology: 05 practicals
Biochemistry
1.1. Concept of Innate and Acquired
immunity, Phygocytosis complement and
Inflammatory responses.

1.2. Immune cells and organs: Structure.

Function and Properties of immune cells -
Stem cell, T-cell, B-cell. NK-cell, Macrophagus,
Neutrophil, Eosinophil, Basophil, Mastcell,
Dentric cell.
 1.3. Immune organ: Bone marrow, Thymus.
Lymph Node. Spleen, Lymphatic System.
And Practicals.
Month Class Paper Topics to be covered No. of
expected
lectures
October B.Sc I Paper I –Cell Unit 4: Immunoglobulins and Immune 20 theory
Year Biology and response: 04 practicals
Biochemistry 1. Immunoglobulins
1.1. Antigens: Characteristics of an antigen:
Foreignness. Molecular size, Chemical
composition and Heterogeneity. Antigen
Adjuvants, Epitopes, Haptens.
1.2. Antibodies: Structure, Types, Functions
and Properties of antibodies Antigenic
determinant on antibodies (Isotypic. Allotypic,
Idiotypic). Monoclonal. Polyclonal and
Chimeric antibody.
2.Immune response:
2.1. Generation of immune response: Primary
and Secondary immune response, generation
of Humoral response (Plasma and Memory
cell).
Generation of cell mediated immune
response (self MHC restriction. T-cell
activation, Co-stimulatory signals). Killing
Mechanisms by CTL and NK cells. Introduction
to tolerance.

And Practicals.
November B.Sc I Paper I –Cell Unit 5: Microbial, Immunological Techniques 22 theory
Year Biology and and Vaccination: 05 practicals
Biochemistry 1. Microbial Techniques:
1.1. Principle. Working and applications of
instruments-Laminar airflow, Autoclave, Hot
air oven.
2. Immunological techniques:
2.1. RIA, ELISA. Western blotting. Principles of
Precipitation. Agglutination, Immunodiffsion.
Immunoelectophorosis.
3. Vaccination:
3.1. Vaccines and vaccination: Rubella,
Varicella(Chickenpox), Polio, Diptheria,
Hepatitis vaccine.

And Practicals.
Dr. Prabhat Kumar Saxena Head of the Department