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Culti-Loops
Sector: Clinical
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OXOID CULTI-LOOPS
Culti-Loops® enable standardised cultures for quality control testing to be prepared
quickly, easily and safely. These ready to use, disposable bacteriological loops contain stabilised viable micro-organisms. For information on organisms available in the range, please download the Culti-Loop Range (58KB) in PDF format .
Culti-Loops are recommended for use in the performance testing of culture media, stains, diagnostic kits and reagents, for the maintenance of stock cultures and in the evaluation of bacteriological procedures. Each loop is individually packaged in a foil pouch, and each pack contains five loops.
Procedure Cut open the end of the foil packet as indicated on the label. The film in each loop is made from a gelatin formulation and then dried by special processing. To rehydrate the film, the loops must come into contact with both warmth and moisture.
Direct inoculation of Culti-Loops onto selective media may result in slow or absent growth. It is therefore recommended that where this is observed, inoculation onto a nonselective medium (such as blood agar) should precede subculture onto selective media.
The following two methods may be used for inoculation. Utilise the appropriate method for the selected micro-organism.
Direct Streak Method
This procedure is recommended for all nonfastidious micro-organisms.
1. Warm the appropriate plate medium to 37°C
2. Remove the sheath from the loop 3. Stab the loop into the medium or lay it flat on the warm, moist surface. Hold it in this manner for 10-15 seconds to allow for absorption of moisture 4. Streak the plate in the usual manner. As many as five plates may be streaked with the same loop 5. Incubate the plates in an appropriate atmosphere and temperature for the optimal growth of the organism.
Indirect (Broth) Method
This procedure is recommended for all fastidious micro-organisms.
1. Remove the sheath from the loop
2. Cut off the loop shaft from the handle using sterilised scissors into a tube containing 0.5 to 1.0 ml of liquid medium. Use: 1. Tryptone Soya Broth (CM0129) or freshly prepared Thioglycollate USP (CM0173) for bacterial specimens. 2. Sterile saline for mycological specimens 3. Place tube in a 37°C incubator just long enough for the film to dissolve completely out of the loop. Shake the tube gently to suspend the organism 4. Using a Pasteur pipette, inoculate appropriate media with several drops and streak in the usual manner 5. Incubate the plates in an appropriate atmosphere and temperature for the optimal growth of the organism
Most organisms grow in 24-48 hours under the proper conditions. However, some exhibit a considerable lag phase and should be incubated for an additional 24 hours.
For further information on the use of Oxoid Culti-Loops, simply download the Quality Control Organisms data sheet (937KB) in PDF format.
Storage Store Culti-Loops at 2-8°C (or frozen for Campylobacter spp.). Remove only the quantity of loops required for immediate use. Under these conditions, Culti-Loops will retain their viability until the date shown on the foil pouch.
Precautions Culti-Loops contain viable micro-organisms and should be used only by individuals with bacteriological training. Each loop should contain an intact dried film. Do not use the loop if there is any evidence of hydration.Refer to national Guidelines for Microbiological Containment Category information. After use, all loops and packaging should be placed into an appropriate container and sterilised by autoclaving before their final disposal. Do not place the loops into bunsen burners.
Disclaimer Those who receive Culti-Loops are responsible for their safe storage, handling and use. Oxoid is not liable for damages or injuries resulting from the receipt and/or use of Culti- Loops. Oxoid is not liable for damages arising from the misidentification or misrepresentation of strains.
Reference Prier J., Bartola E. and Friedman H. (1973) Quality Control in Microbiology. University Park Press, Baltimore.
Note The organisms used in Culti-Loops® are derived from original ATCC® stock cultures according to the number shown. Culti-Loops® are manufactured for Oxoid Ltd by Remel Inc., an FDA approved company (FDA Registration No. 1625984). Culti-Loops® is a registered trademark of Remel Inc.. ATCC® is a registered trademark of American Type Culture Collection. The ATCC Licensed Derivative Emblem™, the ATCC Licensed Derivative word mark®, and the ATCC catalogue marks are trademarks of ATCC. Oxoid Limited is licensed to use these trademarks and to sell products derived from ATCC® cultures. Look for the ATCC Licensed Derivative Emblem™ for products derived from ATCC® cultures.
