Viral diagnostics notes-pdf

Embryonated Hen’s Egg
• Embryonated hen’s egg was first used for cultivation of

viruses by Ernest William Goodpasture.
• The embryonated egg offers several sites for the

cultivation of viruses.
• Specimens are inoculated into pathogen-free fertilised

eggs that are 10-11 days old.
• The eggs are incubated for 2-9 days before harvesting the
viruses.
• Growth and multiplication of the viruses are indicated by

the death of the embryo or by the formation of typical
pocks or lesions on the egg membrane.
Embryonated Hen’s Egg (continued)
Sites and organisms cultured in hen’s eggs

Site Viruses cultured
Chorioallantoic Poxvirus, herpes zoster virus and roux sarcoma virus
membrane
Yolk sac Herpes simplex virus, arbovirus (eg. Japanese encephalitis virus),
bacteria such as chlamydiae and rickettsiae
Allantoic cavity Vaccine production for influenza virus, some paramyxoviruses,
yellow fever (17D strain) and rabies (Flury strain) virus
Amniotic sac Primary isolation of the influenza virus, mumps virus
HBsAg rapid test
• Test: HBsAg rapid test
• Principle of test: lateral flow immuochromatography
• Type of test: Qualitative and screening test
• Test detects the presence of hepatitis B virus surface antigen

(HBsAg) in the patient sample.
• Sample required for testing: Human serum or human plasma
• Interpretation:
Observation Result
Single red line at C (control) Negative
Two red lines at C and T (test) Positive
No red line seen at C Invalid
HIV 1/2 Ab rapid test
• Interpretation:
• Test: HIV 1/2 antibody rapid test
Observation Result
Single red line at C (control) Non-reactive
• Type of test: Qualitative and screening test Two red lines at C and HIV-1 Reactive for
HIV-1
• Test detects the presence of antibodies against Two red lines at C and HIV-2 Reactive for
HIV-1 or HIV-2 in the patient sample. HIV-2
Three red lines at C, HIV-1 & HIV-2 Reactive for
• Sample required for testing: Human serum, HIV-1 & HIV-2
plasma or whole blood
HIV TRI-DOT
• Interpretation:
Observation Result
Single red dot at C (control) Non-reactive
Two red dots at C and HIV-1 Reactive for
HIV-1
• Test: HIV TRI-DOT
Two red dots at C and HIV-2 Reactive for
• Principle of test: flow through assay HIV-2
immunoconcentration Three red dots at C, HIV-1 & HIV-2 Reactive for
HIV-1 & HIV-2
• Type of test: Rapid, qualitative and screening test
No red dot seen at C Invalid
• Test detects the presence of antibodies against
HIV-1 or HIV-2 in the patient sample.
• Sample required for testing: Human serum or plasma

HCV Ab Rapid Test
• Test: HCV antibody rapid test
• Type of test: Qualitative and screening test
• Test detects the presence of antibodies against hepatitis C virus

in the patient sample.
• Sample required for testing: Human serum, plasma or whole blood
• Interpretation:
Observation Result
Single red line at C (control) Non-reactive
Two red lines at C and T (test) Reactive
HCV TRI-DOT
• Interpretation:
Observation Result
Single red dot at C (control) Non-reactive
Two red dots at C and T-1 Reactive for
• Test: 4th Generation HCV TRI-DOT HCV
Two red dots at C and T-2 Reactive for
• Principle of test: flow through assay HCV
immunoconcentration
Three red dots at C, T-1 & T-2 Reactive for
• Type of test: Rapid, qualitative and HCV
in-vitro diagnostic test No red dot seen at C Invalid
• Test detects the presence of antibodies against

hepatitis C virus in the patient sample.

Dengue rapid test
• Test: Dengue test (two device/combo)
• Principle of test: lateral flow immunochromatography
• Type of test: Rapid, qualitative and in-vitro diagnostic test
• Test detects the presence of NS1 dengue antigen and IgM & IgG antibodies against dengue virus in
the patient sample.

Dengue rapid test (continued)
• Interpretation for NS1 antigen: • Interpretation for IgM & IgG antibodies:
Observation Result Observation Result Inference

Single red line at C Negative Single red line at C Negative No Abs for dengue
(control) Red line at C & IgM IgM positive Primary acute
Two red lines at C and Positive dengue infection
NS1 Red line at C & IgG IgG positive Past or secondary
No red line seen at C Invalid dengue infection
Red line at C, IgM & IgM & IgG Secondary dengue
IgG positive infection
No red line at C Invalid

Viral diagnostics notes-pdf

Uploaded by

Copyright:

Available Formats

Viral diagnostics notes-pdf

Uploaded by

Document Information

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Viral diagnostics notes-pdf

Uploaded by

Copyright:

Available Formats

Embryonated Hen’s Egg

• Embryonated hen’s egg was first used for cultivation of

• The embryonated egg offers several sites for the

• Specimens are inoculated into pathogen-free fertilised

• Growth and multiplication of the viruses are indicated by

Sites and organisms cultured in hen’s eggs

• Test: HBsAg rapid test

• Principle of test: lateral flow immuochromatography

• Type of test: Qualitative and screening test

• Test detects the presence of hepatitis B virus surface antigen

• Sample required for testing: Human serum or human plasma

• Sample required for testing: Human serum or plasma

• Principle of test: lateral flow immuochromatography

• Type of test: Qualitative and screening test

• Test detects the presence of antibodies against hepatitis C virus

• Sample required for testing: Human serum, plasma or whole blood

• Test detects the presence of antibodies against

• Sample required for testing: Human serum or plasma

• Test: Dengue test (two device/combo)

• Principle of test: lateral flow immunochromatography

• Type of test: Rapid, qualitative and in-vitro diagnostic test

• Sample required for testing: Human serum or plasma

Observation Result Observation Result Inference

You might also like