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    Lactic Acid Production

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    espada_four
    This document outlines the materials, procedures, and analysis methods for producing lactic acid via fermentation using Lactobacillus sp. The key steps are: 1) Preparing inoculum by growing Lactobacillus in flasks containing glucose, yeast extract, phosphate, and other nutrients. 2) Conducting fermentation in a 2L bioreactor containing corn steep liquor, glucose, yeast extract, and other nutrients to produce lactic acid over 24 hours. 3) Analyzing samples every 4 hours via HPLC to quantify lactic acid and measuring cell growth spectrophotometrically.

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    Lactic Acid Production

    Uploaded by

    espada_four
    438 views4 pages
    This document outlines the materials, procedures, and analysis methods for producing lactic acid via fermentation using Lactobacillus sp. The key steps are: 1) Preparing inoculum by growing Lactobacillus in flasks containing glucose, yeast extract, phosphate, and other nutrients. 2) Conducting fermentation in a 2L bioreactor containing corn steep liquor, glucose, yeast extract, and other nutrients to produce lactic acid over 24 hours. 3) Analyzing samples every 4 hours via HPLC to quantify lactic acid and measuring cell growth spectrophotometrically.

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    This document outlines the materials, procedures, and analysis methods for producing lactic acid via fermentation using Lactobacillus sp. The key steps are: 1) Preparing inoculum by growing Lactobacillus in flasks containing glucose, yeast extract, phosphate, and other nutrients. 2) Conducting fermentation in a 2L bioreactor containing corn steep liquor, glucose, yeast extract, and other nutrients to produce lactic acid over 24 hours. 3) Analyzing samples every 4 hours via HPLC to quantify lactic acid and measuring cell growth spectrophotometrically.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    438 views4 pages

    Lactic Acid Production

    Uploaded by

    espada_four
    This document outlines the materials, procedures, and analysis methods for producing lactic acid via fermentation using Lactobacillus sp. The key steps are: 1) Preparing inoculum by growing Lactobacillus in flasks containing glucose, yeast extract, phosphate, and other nutrients. 2) Conducting fermentation in a 2L bioreactor containing corn steep liquor, glucose, yeast extract, and other nutrients to produce lactic acid over 24 hours. 3) Analyzing samples every 4 hours via HPLC to quantify lactic acid and measuring cell growth spectrophotometrically.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 4

    UNIVERSITI KUALA LUMPUR MALAYSIAN INSTITUTE OF CHEMICAL AND BIOENGINEERING TECHNOLOGY

    FERMENTATION TECHNOLOGY
    (CBB20203)

    MINI PROJECT

    PRODUCTION OF LACTIC ACID


    (MATERIAL & PROCEDURE)

    GROUP

    : MOHD SALEHAN BIN HASSAN


    AHMAD SHAFIQ BIN AHMAD FUAD MOHAMAD NORAZWAN BIN BAHARIN QAIYYUM AZIZI BIN MOHD NOOR

    55202210266 55202210134 55202210381 55202210221

    SECTION LECTURER

    : 3 BCB EX
    : SHARIFAH SOPLAH BT. SYED ABDULLAH

    MATERIAL: A) Bacteria:

    Lactobacillus sp.

    B) Chemical:

    y y y y y y y y

    Glucose. Yeast extract. Ammonium hydrogen phosphate, (NH4)2HPO4 Manganese sulfate, MnSO4 Corn steep liquor. Distilled water. Natrium hydroxide, NaOH. Hydrochloric acid, HCL.

    C) Apparatus:

    y y y y y y y

    2 L Bioreactor Incubator shaker 50 ml Erlenmeyer flask 250 ml Erlenmeyer flask Loop 1l Beaker 1L Measuring cylinder

    METHODS: A) Inoculum:

    Prepare 30 g/L of glucose, 10 g/L of yeast extract, 2g/L of (NH4)2HPO4, and 0.1 g/L of MnSO4 in 1L of distilled water. Adjust culture medium to pH 6.0. Dispense 15 mL in a 50 mL Erlenmeyer flask and autoclave for 15 min at 121OC. Transfer a loopful of Lactobacillus culture from agar slant into the first medium and incubate for 12-14 h at 36 C in incubator shaker at 200 rpm. Prepare another fermentation medium of volume 100 mL in 250 mL and autoclave for 15 min at 121OC. Transfer 2 ml of the inoculum to the flask and incubate for 12-14 h to reach the exponential growth at shaking 200 rpm at 36 C.

    y y y

    B) Fermentation:

    Prepare 30 g/L of corn steep liquor, 75 g/L of glucose, 1.5 g/L of yeast extract, 2g/L of (NH4)2HPO4, and 0.1 g/L of MnSO4 in 1L of distilled water. Adjust fermentation medium to pH 6.0. A 2 L tank bioreactor is used for this fermentation. Transfer 1.35 L of fermentation medium into bioreactor and ready to autoclave for 15 min at 121OC. Transfer 150 ml of innoculum into bioreactor. During all fermentation, agitation speed is fixed at 200 rpm and the temperature within the fermenter was controlled at 36 C and pH at 6.0.

    y y y

    y y

    y y

    Calibrate the pump rate before starting any continuous fermentation. The samples were removed aseptically at regular intervals every 4 hours until 24 hours for further analyses.

    C) Analysis y Lactic acid was quantified by HPLC analysis coupled with a UV variable wavelength detector set to 210 nm. y An Aminex HPX-87H ion-exclusion column was eluted with 5 mM sulfuric acid solution at 0.6 mL/min, and the column temperature was maintained at 35 C. y Cell growth was determined turbidimetrically by a spectrophotometer at 660 nm, and the values thus obtained were then converted to dry cell mass via calculation with the appropriate standard curve.

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