AUTOMATION IN MICROBIOLOGY (For Blood Cultures)
AUTOMATION IN MICROBIOLOGY (For Blood Cultures)
AUTOMATION IN MICROBIOLOGY (For Blood Cultures)
Beginning of Microbiology
Leeuwenhoek
described the first bacteria seen through the microscope, thus providing the technical basis for studying the morphology of microorganisms
The explosion of knowledge in the last century, pioneered by Pasteur, Koch and many others immortalised in modern generic and specific names was dependent on improvements in procedures for isolating and identifying organisms of importance in industry and medicine.
Rapid methods and automation is a dynamic area in applied microbiology dealing with the study of improved methods in the isolation, early detection, characterization, and enumeration of microorganisms and their products in clinical, food, industrial, and environmental samples.
Changing perceptions,
Microbiology labs are beginning to accept the shift toward automation for reasons as numerous and varied as microbiology itself. Microbiologists and lab technicians recognize that automated solutions are not intended to replace cognitive decision-making but rather, simply replace tedious, repetitive steps.
Man has Evolved So also the Microbes, so the need for Automation
Beginning of Automation
The field started around mid-1960s and with the development of a variety of miniaturized microbiological techniques developed Streamlining workflow maintains consistency but allows microbiologists to devote more time to operations that require their unique skills and experience
Hospital Acquired Infection a threat to Medical Profession- needs faster methods to Identify.
Microbiology labs play a crucial role by establishing a front line of defence against the threat of Nosocomial infections. According to the Centres for Disease Control and Prevention, in American hospitals alone, HAIs account for an estimated 1.7 million infections and 99,000 associated deaths annually. Of these infections, 32% are urinary-tract, 22% are surgical-site, 15% are pneumoniae, and 14% are bloodstream.
The shift from manual test processing to automated solutions can aid in reducing HAIs by providing health systems with standard, consistent lab processing that yields quicker, more accurate test results.
Rapid Methods and Automation in Microbiology has developed into an important sub-discipline of applied microbiology in the past 15 years. The field deals with improved methods in the isolation, early detection, characterization, and enumeration of microorganisms and their products in clinical, food, industrial, and environmental samples.
Automated processes have gone well beyond changing outdated procedures. They minimise potentially dangerous practices, lower turnaround time, reduce errors, enhance quality control, improve specimen handling and boost accuracy. Moreover, technologists in automated labs tend to embrace innovation more readily.
Because automation largely circumvents repetitive manual processing, lab technicians find it easier to focus on complex tasks that require their specific skills. It is therefore remarkable that, despite shortages of skilled personnel and increases in the volume of work, it has taken years for automation to become acceptable to microbiologists.
Laboratory automation for improved efficiency and error reduction Create an informatics continuum
Lab Test
Faster TOT Accuracy, Precision, Safety
Trending
Life Style Adjustments Appropriate Therapeutics
infection
Physician Physician
can initiate life-saving support measures can start targeted antibiotic therapy
A blood culture is a laboratory test in which blood is injected into bottles with culture media to determine whether microorganisms have invaded the patients bloodstream.
A clinically suspected infection is ultimately confirmed by isolation or detection of the infectious agent. Subsequent identification of the microorganism and antibiotic susceptibility tests further guide effective antimicrobial therapy. Bloodstream infection is the most severe form of infection and is frequently life-threatening, and blood culture to detect circulating microorganisms has been the diagnostic standard.
Blood culture is a microbiological culture of blood. It is employed to detect infections that are spreading through the bloodstream
(such as bacteraemia, septicaemia amongst others).
Most microbiological culture procedures require the use of solid media, like blood agar and MacConkey agar plates that need to be visually monitored by trained personnel at intervals of 24 hours. These conventional cultures using normal media take at least a minimum of 72 hours to isolate the pathogen and carry out susceptibility test to know the efficacy of antibiotics on simple aerobic bacteria
Phlebotomist
Defined as a person who collects blood for clinical laboratory test or examination purposes
Gloves will be worn in accordance with standard precautions. A physicians order must be obtained for specimen collection. Appropriate verification of the patient's identity, by means of an armband or area specific procedure, will occur before the specimen collection. Cultures should be drawn before administration of antibiotics, if possible. If at all possible, blood cultures should note drawn from lines, but should be drawn viavenipuncture.
Materials
Chlorhexidine swabs (1-2 packages) Alcohol swabs Blood culture bottles (2 bottles per set) 2 syringes (adult: 20 cc, paediatric: 5 cc) 2 needles (adult: 22 gauge or preferably larger butterfly or standard needle; pediatric: 25 or 23 gauge butterfly or standard needle) Gloves (sterile &nonsterile) Tourniquet Sterile gauze pad Adhesive strip or tape Self-sticking patient labels Plastic zip lock specimen bags
1.Identify the patient by checking the arm band or area-specific procedure. 2.Explain the procedure to the patient. 3.Wash hands with soap and water with friction for 15 seconds or use alcohol based hand rub
Prep the rubber cap of the blood culture bottles with an alcohol pad in a circular motion. Allow the alcohol to dry.
Do not wipe the site after cleansing the skin with Chlorhexidine.
Step 6 -Gloves
Apply gloves: If palpation of site prior to puncture is anticipated, wear sterile gloves. If palpation of site prior to puncture is not anticipated, wear nonsterile gloves.
Step 8 -Mix
Gently rotate the bottles to mix the blood & the broth (do not shake vigorously).
Step 11
11.Document the following in the medical record Date & time specimen obtained Site of specimen collection If 2 sets of blood cultures have been ordered, obtain the second set in the same manner as the first, making a new venepuncture at a different site
Tourniquet Application
Need to maximize chances of successful collection Remember that the vein is still developing and might need to rely on firm tightness Ideally, tourniquet should not be kept on for more than one minute If possible, apply heat If using a hand, consider a bucket of warm water
Insertion Principles
Cantilevering of elbow The option factor:
-Choose your options of direction before insertion -Minimize the odds of unnecessary digging
Avoid plunging the needle right up to the hilt Often, a drawback does the trick If vacutainer is slowing down, replace with a syringe
Self Protection
A few ways to make sure your role in the collection process is carried out with efficiency, orderliness and safety
Process Control
Reporting
Storage
RFID 2D-Codes
Recording Biorepository
Analysis
Pre-analytical Analytical and Automation
Transportation
Mobile Robot
Accessioning
A blood culture is a laboratory test in which blood is injected into bottles with culture media to determine whether microorganisms have invaded the patients bloodstream.
Blood cultured by the BacT/Alert 3D leads to early detection of pathogens (>89 per cent within 24 hours and 97 per cent within 48 hours) especially in cases of septicaemia, enteric fevers, bacterial endocarditis and other pyrexias of bacterial origin. Activated charcoal neutralises antimicrobials and toxins enhancing early recovery of pathogens. Positives are detected faster than Bactec even at low concentrations in blood and body fluids like CSF, CT guided aspirates etc. Delayed transport does not compromise results. The instrument is capable of recovering significantly more organisms that resin.
BacT/AlerT 3D culture system. This is the first automated non-radiometric and noninvasive culture system that continuously monitors system for culture of bacteria
(both aerobic and anaerobic), fungi and mycobacteria. All these bacteria can be
cultured using different media as prescribed..
Principles in BacT/AlerT 3D
culture system
This is a closed system and works on the colorimetric principle of detection of CO2 produced by the organisms. The CO2 causes a lowering of the pH of the medium, which in turn produces a colour change in a sensor attached to the CO2sensitive base of each bottle.
The instrument reacts before this colour change is apparent by means of an audible or visible alert flagged by the computer. The bottles are constantly agitated and are read at 10-minute intervals. The readings are transmitted to a computer compiler, which computes results. This
bioMrieux BacT/ALERT 3D
The bioMrieux BacT/ALERT 3D provides an optimal environment for the recovery of a wide range of pathological organisms, including bacteria, yeasts and mycobacteria; utilizing proprietary plastic culture bottles ensuring added safety to the user.
Microorganisms multiply in the media, generating CO2. As CO2 increases, the sensor in the bottle turns a lighter colour. Measuring reflected light, the BacT/ALERT 3D monitors and detects color changes in the sensor.
Algorithms analyze the data to determine positivity, and the laboratory is notified immediately with visual and audible alarms.
Full microbiology laboratory automation needs have never been so apparent, with financial constraints and increasing testing volumes at the same time that labour is becoming both harder to find and more expensive. Implementation of full microbiology lab automation is one solution, as fewer technologists are required to process automated tests..
Overall, laboratories transitioning from conventional to automated processes find that technologists and microbiologists are more open to innovation and improved quality.
An entire industry of microbial diagnostic kits flourished to the present day. Next in the 70s the development was in immunological test kits and instruments to monitor the presence of food borne pathogens and biomass and to predict microbial growth automatically.
Advantages of automation
Automated solutions have recently emerged in the marketplace that address key areas of the microbiology lab. Automating these processes-simple, standard, or complex-can revolutionize the microbiology lab with more efficient, standardized practices that will improve quality, safety, and cost-efficiency.
For example, automating small, yet vitally important tasks, can make a huge impact on the efficiency and accuracy of laboratories. Lab technicians streak an estimated ??? agar plates a day, a process that is laborious, tedious, and inconsistent. Each lab technician has his own streaking technique
Automation is Advantageous
Include the elimination of subjective variability, Savings in media and reagents, and the earlier production of useful information in many instances, all of winch can make a substantial contribution to productivity and the control of runaway cost escalation.
The combined use of laboratory automation and laboratory information management software (LIMS) has been shown to increase productivity, reduce human error and improve tracking and traceability in a microbiology lab
Workflow Management
Automation
Information
Data
DATA
Accessioning Specimen tracking Data logging and reporting Quality control documentation
PROCESS MANAGEMENT
Bar coding, robotics and computers that replace manual transcription significantly reduce data loss and errors. Automation also makes it less likely that plate information and patient identification will be duplicated or transposed. Three trends will drive laboratory automations future: smaller, more-flexible analysers and automation based on next-generation technology, including micro fluidics, easy-to-use, powerful software for centralised lab management, and internet-based real-time service for better up-time.
The key to this real-time automation was real time barcode labelling of all sample carriers (such as bags, tubes, dishes, bottles) provided by Kiestra's Barcode system. This was used in conjunction with Auto scribe's Matrix LIMS. Real time barcode reading is known to reduce transcription error rates to only 1 in 36 trillion characters - compared to 1 in 300 characters with manual reading.
Kiestra's BarcodA
Kiestra's BarcodA automatically places an optical barcode on all tubes, bottles and petri-dishes that contains important information such as composition, sell-by date etc.
The barcode makes every sample carrier unique and recorded meaning full traceability for the laboratory. Samples are also provided with a barcode which is generated by Matrix LIMS.
Quality issues are becoming increasingly important in diagnostic laboratories. The fact of quality is no longer sufficient and we must now develop mechanisms to assure consumers, the public and, most importantly, ourselves of the continuing quality of our service. Moving towards a quality-assured system is not easy, requiring a meticulous attention to detail in all areas of a laboratory's working and organization.
A further plus for lab automation is that it promotes consistency and quality. Without automation, lab tasks that are necessarily repetitive can lead to inconsistent or inappropriate ways of work and, from there, to improper treatment, longer patient stays, medication errors and unwanted drug side-effects.