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    Sime Darby Nursing and Health Sciences College: Diploma in Medical Laboratory Technology July 2012 Intake

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    Salmonella typhi is a bacterium that causes typhoid fever in humans. It is transmitted through the fecal-oral route. S. typhi is gram-negative, facultatively anaerobic rod shaped bacteria. It can be identified through culture media such as MacConkey agar and biochemical tests including the indole, methyl red, Voges-Proskauer, and citrate tests. Diagnosis is confirmed through isolation from blood, stool, or urine specimens and serological tests such as the Widal test.

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    Sime Darby Nursing and Health Sciences College: Diploma in Medical Laboratory Technology July 2012 Intake

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    29 views34 pages
    Salmonella typhi is a bacterium that causes typhoid fever in humans. It is transmitted through the fecal-oral route. S. typhi is gram-negative, facultatively anaerobic rod shaped bacteria. It can be identified through culture media such as MacConkey agar and biochemical tests including the indole, methyl red, Voges-Proskauer, and citrate tests. Diagnosis is confirmed through isolation from blood, stool, or urine specimens and serological tests such as the Widal test.

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    Salmonella typhi is a bacterium that causes typhoid fever in humans. It is transmitted through the fecal-oral route. S. typhi is gram-negative, facultatively anaerobic rod shaped bacteria. It can be identified through culture media such as MacConkey agar and biochemical tests including the indole, methyl red, Voges-Proskauer, and citrate tests. Diagnosis is confirmed through isolation from blood, stool, or urine specimens and serological tests such as the Widal test.

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    Download as pptx, pdf, or txt
    29 views34 pages

    Sime Darby Nursing and Health Sciences College: Diploma in Medical Laboratory Technology July 2012 Intake

    Uploaded by

    visa
    Salmonella typhi is a bacterium that causes typhoid fever in humans. It is transmitted through the fecal-oral route. S. typhi is gram-negative, facultatively anaerobic rod shaped bacteria. It can be identified through culture media such as MacConkey agar and biochemical tests including the indole, methyl red, Voges-Proskauer, and citrate tests. Diagnosis is confirmed through isolation from blood, stool, or urine specimens and serological tests such as the Widal test.

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    SIME DARBY NURSING AND HEALTH SCIENCES COLLEGE

    DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY JULY 2012 INTAKE

    MEDICAL MICROBIOLOGY 2 MLTC 1222


    Salmonella typhi
    PRESENTED BY VISANATHAN A/L SEVANATHAN DML/0542/12

    HISTORY

    Genus- Salmonella
    Was named after Daniel Elmer Salmon, an American veterinary pathologist (1850-1914) Theobald Smith was the actual discover of the type Bacterium (Salmonella enterica var. cholerasuis) in 1985

    Daniel Elmer Salmon

    Theobald Smith

    Species- Salmonella typhi


    Typhi got its name from Typhoid fever that caused by the typhoid bacillus Typhoid bacillus was 1st observed by Eberth (1880) and was isolated by Gaffky (1884) Known as the Eberth Gafky bacillus or Eberthella typhi

    INTRODUCTION

    Salmonella typhi is only can be found in human beings It is a type of bacterium that can cause typhoid fever in human beings

    CLASSIFICATION

    Kingdom: Eubacteria
    Phylum: Proteobacteria

    Class: Gammaproteobacteria
    Order: Enterobacteriales Family: Enterobacteriaceae Genus: Salmonella Species: S. typhimurium

    MORPHOLOGY

    Gram-negative rod (bacilli bacteria) Diameter : 0.7 - 1.5 microns Length : 2 - 5 microns Facultatively anaerobic Non-sporing Non-capsule

    Motile (peritrichous flagella) Chemoorganotroph Do not form spores

    Salmonella typhi

    Salmonella typhi

    CULTURE MEDIA

    Salmonella typhi in Macconkey Agar

    Salmonella typhi in Wilson Blair Medium

    Xylose Lysine Deoxychocolate (XDL) Agar

    BIOCHEMICAL TESTS

    Indole Test

    Methyl Red Test


    Voges Proskaeur Test Citrate Test

    Indole Test

    Methyl Red Test

    Voges-Proskaeur Test

    Citrate Test

    SEROLOGICAL TESTS

    Widal Test :
    Tube dilution tests Measure agglutinating antibodies against O and H antigens. The Vi antigen is used

    PATHOGENESIS

    Salmonella infections are a zoonotic disease meaning that the infection can spread between animals and people Salmonella is transmitted from animals to humans and humans to humans by the fecal oral route. Salmonella can be passed from one person to another through poor hygiene. Example, Salmonella can be passed from one person to another by not properly washing hands after using the bathroom or after changing a diaper

    LABORATORY ANALYSIS:
    SPECIMEN COLLECTION
    CONFORMATION ANALYSIS

    SPECIMEN COLLECTION

    Main specimens:
    Whole blood/Clotted blood Stool

    Urine

    Other specimens: Bone marrow Cerebrospinal fluid Culture of bile obtained by duodenal aspiration

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