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    Journal Review The Effect of Aeration Conditions On The Fermentation Kinetics of Bacillus Thuringiensis Kurstaki

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    suci apsari pebrianti
    Effect of aeration on volumetric mass transfer coefficient to determine the optimum conditions for delta endotoxin production

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    Journal Review The Effect of Aeration Conditions On The Fermentation Kinetics of Bacillus Thuringiensis Kurstaki

    Uploaded by

    suci apsari pebrianti
    13 views23 pages
    Effect of aeration on volumetric mass transfer coefficient to determine the optimum conditions for delta endotoxin production

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    Journal review The effect of aeration conditions on the fermentation kinetics of bacillus thuringiensis kurstaki

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    Effect of aeration on volumetric mass transfer coefficient to determine the optimum conditions for delta endotoxin production

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    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    13 views23 pages

    Journal Review The Effect of Aeration Conditions On The Fermentation Kinetics of Bacillus Thuringiensis Kurstaki

    Uploaded by

    suci apsari pebrianti
    Effect of aeration on volumetric mass transfer coefficient to determine the optimum conditions for delta endotoxin production

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
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    You are on page 1of 23

    Marta Tika Handayani 17/PTP/419/015

    Suci Apsari Pebrianti 17/PTP/419891/01556


    INTRODUCTIONS

    Chemical insecticidal
    Natural or bioinsecticidal
    δ-endotoxin
    Produced by Bacillus thuringiensis during sporulation

    cost production Byproduct medium : CMB


    cost energy consumption Constant operating conditions
    toxin productivity

    2% Dissolved oxygen (DO) as a key parameter

    Volumetric mass transfer coefficient (KLa) Aeration flow


    THEORETICAL BASIS
    Sporulating cell
    Bacillus thuringiensis kurstaki

    Rod-shape, spore-forming bacteria, facultative anaerobe TEM


    And Gram +

    Sporulation phase
    δ-endotoxin production Cell lysis
    Late
    sporulation
    phase

    δ-endotoxin
    release SEM

    Vegetative growth
    Dissolved oxygen
    Volumetric mass transfer coefficient (KLa)
    THE OBJECTIVE OF THE RESEARCH

    To study and analyze the effect of aeration on the fermentation kinetics of Bacillus
    thuringiensis kurstaki (Btk) cultivated in cereal milling byproduct (CMB) mono-
    component medium in order to determine the optimum conditions for improving
    the δ-endotoxin productivity.

    THE ADVANTAGES OF THE RESEARCH

    Can be used as reference and guideline to determine the optimum fermentation


    condition of Bacillus thuringiensis kurstaki using CMB medium to produce δ-endotoxin
    with high yield and productivity and cost effective.
    MATERIALS AND METHODS

    Preparation of seed culture and culture conditions at different KLa

    2% v/v
    B.Thuringiensis
    kurstaki 100 mL Luria 98 mL Tryptose
    from soil Bertani broth Phosphate broth

    30oC, 340 rpm 30oC, 340 rpm


    8 h incubation 12 h incubation

    50 mL

    Analysis
    Tryptose
    Phosphate broth

    1.45 L CMB (6%) medium at 30oC and different aeration flow


    Culture conditions at optimum KLa and different CMB ratios

    50 mL

    Analysis
    Tryptose 3% CMB 6% CMB 9% CMB
    Phosphate broth

    1.45 L CMB medium at 30oC and optimum KLa value


    Analytical Methods

    Cell, spores and crystal protein (δ-endotoxin) counts


    microscopically determined by Petroff hausser counting chamber

    Toxin protein estimation


    determined by Bradford method

    Oxygen Transfer Rate Oxygen Uptake Rate Specific Oxygen Transfer Rate

    Specific growth rate Toxin protein Productivity

    Cell yield based on O2 consumption


    RESULTS AND DISCUSSIONS
    Growth, sporulation and δ-endotoxin production in a 6% CMB culture medium at different values of kLɑ
    Variations of the CO2 percent in the effluent gazes
    pH variations at different values of kL ɑ

    The initial pH was 6,2 and slight variations were observed in all studies condition
    with a pH in the range of 5,6 – 6,8 through out the fermentation, except for kL ɑ
    equal to 7,2 h-1 where the pH decrease reach a low values 5,4
    Variations of the specific growth rate and the maximum specific oxygen uptake rate for
    the different values of kL ɑ
    Productivity
    Cell yields based on O2

    Decreased from 2,63.1012 to 1,38.1012 cell/g consumed O2 when kL ɑ was


    increased from 13,3 h-1 to 106,2 h-1
    Growth, sporulation and δ-endotoxin production for different CMB ratios
    Conclusions

    The best toxin proteins productivity (85.7 mg/L/h) was reached in 6% CMB
    culture medium for KLa of 65.5 h -1.
    Komentar

    • Peneliti tidak menampilkan data analisa pH tidak ditampilkan dan hanya


    menyebutkan range pH saja. Sehingga pembaca kesulitan untuk melihat pola
    penurunan pH nya.
    • Data sugar consumption tidak ditunjukkan, sedangkan peneliti membahas tentang
    hubungan pH dan sugar consumption.
    • Pada bagian Growth, sporulation and δ-endotoxin production for different CMB
    ratios, data yang ditampilkan tidak lengkap.
    Terima Kasih
    Stage I: axial filament formation; Stage II: forespore formation;
    Stage III: engulfment; Stage IV-VI: formation of cell wall, cortex,
    and spore coats accompanied by transformation of the spore
    nucleoid; Stage VII: spore maturation

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