This study aims to test the effectiveness of Dalanghita peel oil extract as a larvicide against Aedes aegypti mosquitoes. The researchers will expose A. aegypti larvae to three different concentrations of Dalanghita peel oil extract (50%, 75%, 100%) and measure mortality rates after 24 and 48 hours. They hypothesize that the extracts will not have a significant larvicidal effect. If effective, Dalanghita peel extract could serve as a low-cost natural pesticide for remote areas in the Philippines. Statistical analysis using ANOVA will determine if mortality rates differ significantly between concentrations.
This study aims to test the effectiveness of Dalanghita peel oil extract as a larvicide against Aedes aegypti mosquitoes. The researchers will expose A. aegypti larvae to three different concentrations of Dalanghita peel oil extract (50%, 75%, 100%) and measure mortality rates after 24 and 48 hours. They hypothesize that the extracts will not have a significant larvicidal effect. If effective, Dalanghita peel extract could serve as a low-cost natural pesticide for remote areas in the Philippines. Statistical analysis using ANOVA will determine if mortality rates differ significantly between concentrations.
Original Title
Larvicidal Effect of Dalanghita (Citrus nobilis).pptx
This study aims to test the effectiveness of Dalanghita peel oil extract as a larvicide against Aedes aegypti mosquitoes. The researchers will expose A. aegypti larvae to three different concentrations of Dalanghita peel oil extract (50%, 75%, 100%) and measure mortality rates after 24 and 48 hours. They hypothesize that the extracts will not have a significant larvicidal effect. If effective, Dalanghita peel extract could serve as a low-cost natural pesticide for remote areas in the Philippines. Statistical analysis using ANOVA will determine if mortality rates differ significantly between concentrations.
This study aims to test the effectiveness of Dalanghita peel oil extract as a larvicide against Aedes aegypti mosquitoes. The researchers will expose A. aegypti larvae to three different concentrations of Dalanghita peel oil extract (50%, 75%, 100%) and measure mortality rates after 24 and 48 hours. They hypothesize that the extracts will not have a significant larvicidal effect. If effective, Dalanghita peel extract could serve as a low-cost natural pesticide for remote areas in the Philippines. Statistical analysis using ANOVA will determine if mortality rates differ significantly between concentrations.
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Group Leader: Contreras, Christian Alexis L.
Group Members: Mendova, Andrea C.
Santos, Joshua Troy Suarez, Viva Gia T. Tolentino, Ma. Elaisa M. Introduction Mosquitoes have always been a danger that people have to deal with. A mosquito bite can transmit Dengue and it can also transmit the Zika virus, Chikungunya virus, Malaria virus, Rickettsia infection and other various parasites and diseases they harbor. Larvicide however can be quite costly for the remote areas in the Philippines. 20 kilograms of Larvicide can go range from P7920, if imported from outside the country. With these facts, the researchers found that citrus fruits such as Dalanghita are of high medicinal value because of their multiple uses, such as in the food industry, cosmetics and folk medicine. Dalanghita peel oil extract which contains a considerable amount of limonene, has numerous applications including natural insect repellent for use on humans. Their waste such as their peelings can be used in the production of other by- products. The researchers would like to prove that the dalanghita peel oil extract can be economically viable and effective answer to the mosquito proliferation to prevent another outbreak occurs. Statement of the Problem What is the mortality rate for the A. aegypti larvae using Dalanghita peel oil extract after 24 hours? And 48 hours of exposure period? Which of the following extract concentrations would exhibit optimum larvicidal effect? (50% g/mL, 75% g/mL, 100% g/mL) Is the dalanghita peel oil extract effective in being a larvicidal agent against Aedes aegypti larvae? Statement of Hypothesis Ho: Dalanghita peel oil extracts will not leave a significant effect as a larvicide in the 50%, 75%, and 100% extract in all concentrations. Conceptual Framework (Schematic Diagram) Significance of the Study For the people in the Medical Field, this study will provide these people with basic knowledge and information as to the potential use of natural pesticide. For the students, this study will provide information for them to become critical thinkers. For the researchers, this study will provide an open opportunity for the future researchers to conduct further investigation related to the problem. Scope and Delimitation of the Study • This study aims to use the peelings of dalanghita as an alternative larvicidal agent. This study will only focus on the effectiveness of the dried peelings of dalanghita as a larvicidal. The researcher does not aim to analyze other possible outcomes that can be done with the dalanghita peel. Method of Research Research Design The study uses an experimental method of research which includes: * collection of plant sample * collection of plant extraction The result shows proof of the larvicidal effect Dalanghita peel oil extracts towards Aedes aegypti larvae. Methods of Research Instrumentation and Validation The researchers will use dalanghita fruits that will be collected from Calamba market and will be authenticated by the botanist of the Botanical Herbarium of the University of the Philippines Los Baños Laguna. The Aedes aegypti larvae will be purchased and verified from the Institute of Biological Sciences of the University of the Philippines Los Baños Laguna. The dalanghita peels will be extracted using Soxhlet apparatus that are made of Pyrex glass, with high thermal resistance Statistical Analysis The statistical tools were employed for the analysis and interpretation of data. The one-way analysis of variance (ANOVA) is used to determine whether there are any statistically significant differences between the means of two or more independent (unrelated) groups. This will be used to determine if there is significant difference in mortality rate among different dalanghita peel extract concentrations.