THE GEOMETRY CONTROL OF 3D

PCL SCAFFOLD BY A
COMBINATION OF
PARTICULATES LEACHING FOR
BONE TISSUE ENGINEERING
APPLICATIONS
STUDENT: TRAN NGUYEN TUYET NGAN
INSTRUCTOR: ASS.PROF. NGUYEN THI HIEP
CONTENTS

I. INTRODUCTION
II. METHODOLOGY
III. RESULTS & DISCUSSION
IV. CONCLUSION

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INTRODUCTION

Global Dental Bone Graft Substitutes Market Worth Reach USD 931 Million By 2025- MENAFN - Zion
3
Market Research 2019
 INTRODUCTION
There are many kinds of bone graft in orthopedic surgery, each has advantages and
disadvantages.

*Image and information from Voss Dental (Houston) website.

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 INTRODUCTION

TOPIC 01 TOPIC 02

ALLO/AUTO/XENO GRAFTS SYNTHETICS GRAFT

• Limited donor source, costly • Polymer base graft need
• Limited donor site, pain researching

• Rejection

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INTRODUCTION
• Dental pulp stem cells (DPSC)
• Stem cells from human exfoliated
deciduous teeth (SHED)

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INTRODUCTION

PARTICULATE LEACHING ;
POROGENS

• Particulate leaching : easy pore control

• Degradable
• Porogens are easily leached out by water:
• Biocompatible
a. NaCl: creates micropores 100-300µm []
• Good mechanical properties:
b. Sucrose : creates macropores from 500 µm []
Compression and reform
c. Monosodium glutamate: rod-shape, expected
to raise degree of interconnection, non-toxic
to cells. PCL

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METHODOLOGY
Particulate leaching

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METHODOLOGY

Figure 1: Fabrication of 3D PCL scaffolds 9

METHODOLOGY

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RESULTS & DISCUSSION

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RESULTS & DISCUSSION

Figure 2. a) NaCl (N) b) Sucrose (S) c)MG (M)

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 RESULTS & DISCUSSION

A B C D

Figure 3 . PCL concentration : A) PCL 30%,B) PCL35%, C) PCL40% and D) PCL45%

(NaCl-PCL (1-1)(w/w))

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RESULTS & DISCUSSION

A B D E

Figure 4: SEM 35x of PCL scaffolds by different porogens A) MN; B) MS; C) SN; D) MSN;

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RESULTS & DISCUSSION

Count Count Coun

Count t (%)
(%) (%) (%) MSN
MN MS SN
Mean=0.61 Mean=0.55 Mean=0.52
Mean=0.42
St.dev=0.28 St.dev=0.31 St.dev=0.22
St.dev=0.23
N=51 N=52 N=63
N=50

Diameter (µm) Diameter (µm) Diameter (µm) Diameter (µm)

Figure 5: Pore size distributions. A) MN; B) MS; C) SN; D) MSN.

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RESULTS & DISCUSSION

Table 1: Pore diameter distribution summary of 3D PCL scaffolds made by different combination

  MN MS SN MSN
Sample Size 52 53 51 64

Normal
Failed Failed Failed Passed
distribution

Median 571 476 391 467

Outlier value 3 1 0 0
25%-75% 421-748 312-775 245-611 348-667

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RESULTS & DISCUSSION
0.4

0.35

0.3

0.25
Stress (Mpa)

0.2
MN
MSN
0.15 SM
SN

0.1

0.05

0
0 500 1000 1500 2000 2500 3000 3500 4000 4500

-0.05

Strain (%)

Figure 6: Stress-strain curves for PCL scaffold by different mixture of

porogens. 17
RESULTS & DISCUSSION
100

90
90

80
80
76.5
Recovery (%)

70

60

50

40
40

30
MN MS MSN SN

Figure 7:Percentage recovery for the scaffolds after a deformation of 80%.

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RESULTS & DISCUSSION
A

Figure 8A: Image of water droplet, from left to right is SN, MN, MSN,
MS; 8B) Contact angles 19
RESULTS & DISCUSSION

Figure 9: Water absorption rate of PCL scaffolds

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RESULTS & DISCUSSION
3000

2500

Time (s)
2000

1500 SN
SM
MN
MSN
1000

500

0
1st 2nd 3rd 4th

Drop

Figure 10: Water absorption rate of scaffolds

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RESULTS & DISCUSSION
110

108

106
Cell viability (%)

104
0

102
12.5

100 25

98 50

100
96

94
MN SN MS MSN

Extract concentration (%)

Figure 12: Cell viability in different concentrations of extract solution of the scaffolds.
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 CONCLUSION

• Morphologies: Various shapes of pore and the pore size range is wide,
macropore (>300µm) is dominant, which suitable for various further
researches on vascularization.
• Structure: high porosity but low compressive strength.
• Cell-materials interaction: non-toxic to cells

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