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    DNA Extraction

    Uploaded by

    khdabhi
    This document provides instructions for extracting DNA from peas in 3 easy steps: 1. Blend peas with salt and water to create a pea-cell soup, then strain and mix with detergent. 2. Add meat tenderizer enzymes and stir gently. 3. Pour rubbing alcohol on top to form separate layers, and the DNA will rise into the alcohol layer where it can be drawn out.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd

    DNA Extraction

    Uploaded by

    khdabhi
    2K views8 pages
    This document provides instructions for extracting DNA from peas in 3 easy steps: 1. Blend peas with salt and water to create a pea-cell soup, then strain and mix with detergent. 2. Add meat tenderizer enzymes and stir gently. 3. Pour rubbing alcohol on top to form separate layers, and the DNA will rise into the alcohol layer where it can be drawn out.

    Original Description:

    It describes simple procedure to extract DNA from green peas at home.

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    © Attribution Non-Commercial (BY-NC)

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    This document provides instructions for extracting DNA from peas in 3 easy steps: 1. Blend peas with salt and water to create a pea-cell soup, then strain and mix with detergent. 2. Add meat tenderizer enzymes and stir gently. 3. Pour rubbing alcohol on top to form separate layers, and the DNA will rise into the alcohol layer where it can be drawn out.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    2K views8 pages

    DNA Extraction

    Uploaded by

    khdabhi
    This document provides instructions for extracting DNA from peas in 3 easy steps: 1. Blend peas with salt and water to create a pea-cell soup, then strain and mix with detergent. 2. Add meat tenderizer enzymes and stir gently. 3. Pour rubbing alcohol on top to form separate layers, and the DNA will rise into the alcohol layer where it can be drawn out.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
    Download as ppt, pdf, or txt
    of 8

    DNA Extraction

    DR. K.H.Dabhi
    Asst. Research Scientist
    Dept. of Agricultural Botany
    JAU, Junagadh
    Put in a blender
    • The blender separates the pea
    cells from each other, so you
    now have a really thin pea-cell
    soup.
    • Your DNA source (about
    100ml or 1/2 cup of split peas)
    • A large pinch of table salt (less
    than 1ml or 1/8 teaspoon)
    • Twice as much cold water as
    the DNA source (about 200ml
    or 1 cup)
    • Blend on high for 15 seconds.
    And now, 3 easy steps: 1
    • Pour your thin pea-cell
    soup through a strainer
    into another container
    (like a measuring cup).
    • Add about 1/6 of that
    amount of liquid Detergent
    (about 30ml or 2
    tablespoons) and swirl to
    mix. Let the mixture sit for
    5-10 minutes.
    • Pour the mixture into test
    tubes or other small glass
    containers, each about
    1/3 full.
    And now, 3 easy steps: 2
    • Add a pinch of enzymes
    to each test tube and stir
    gently. Be careful! If you
    stir too hard, you'll break
    up the DNA, making it
    harder to see.
    • Use meat tenderizer for
    enzymes. If you can't find
    tenderizer, try using
    pineapple juice or contact
    lens cleaning solution.
    And now, 3 easy steps: 3
    • Tilt your test tube and
    slowly pour rubbing
    alcohol (70-95% isopropyl
    or ethyl alcohol) into the
    tube down the side so that
    it forms a layer on top of
    the pea mixture. Pour until
    you have about the same
    amount of alcohol in the
    tube as pea mixture.
    •
    DNA will rise
    • DNA will rise into
    the alcohol layer
    from the pea layer.
    You can use a
    wooden stick or
    other hook to draw
    the DNA into the
    alcohol.
    What is that stringy stuff?
    • Alcohol is less dense than
    water, so it floats on top. Since
    two separate layers are formed,
    all of the grease and the protein
    that we broke up in the first two
    steps and the DNA have to
    decide:
    "Hmmm...which layer should I
    go to?"

    • The protein and grease parts


    find the bottom, watery layer
    the most comfortable place,
    while the DNA prefers the top,
    alcohol layer.
    • DNA is a long, stringy molecule
    that likes to clump together.
    Congratulations! You have just
    completed a DNA extraction!

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