Course: Post Harvest Management and

Value Addition of Fruits and Vegetables

(6th Semester)
Lecture 2: Fruit Ripening and Changes occurring
during Ripening
Presented by
Dr. Asmita
Department of Horticulture
BAU, Ranchi
e-mail: asmitakumari97@gmail.com
 Fruit ripening is a genetically programmed stage of
development overlapping with senescence.
 The fruit is said to be ripe when it attains its full flavour
and aroma and other characteristics of the best fruit of
that particular cultivar.
 The words “mature “and “ripe” are essentially
synonymous when used to describe these fruits that ripe
on the plants known as non-climacteric.
 However, in case of climacteric fruits, a mature fruit
require period before attaining a desirable stage of
edibility.
 Climacteric and Climacteric Non-climacteric fruits
Climacteric fruits Non-climacteric fruits
That can ripen after being Will not ripen, once separated
picked/harvested. from the plant.
Enhanced rate of ethylene Produce very small amount of
production and respond to ethylene and don’t respond to
ethylene treatment. ethylene treatment.
Increased rate of respiration or No characteristics increased
production of carbon dioxide. rate of respiration or
production of carbon dioxide.
Apple, Apricot, Avocado, Banana, Carambola, Cherries, Citrus,
Ber, Cherimoya, Fig, Guava, Grape, Litchi, Loquat, Olive,
Kiwifruit, Mango, Melons, Pear, Pineapple, Pomegranate,
Peach, Plum, Persimmon, Papaya, Strawberry are non-climacteric
Tomato, Sapota, Passion fruit are fruits.
the climacteric fruits.
Green Banana
 Changes during Fruit Ripening
Cell Wall Changes
Starch
Organic acids
Colour
Flavouring
Compounds
Ascorbic Acid
Phenolics
Amino Acids and
Proteins
Respiration
 Changes during Fruit Ripening
Cell Wall Changes
 Cell wall consists of pectic substances and cellulose
as the main components along with small amounts
of hemicellulose and non-cellulosic polysaccharides.
 In cell wall, the changes particularly in the middle
lamella which is rich in pectic polysaccharides are
degraded and solubilized during ripening.
 During this softening, there is a loss of neutral sugars
(galactose and arabinose-major components of
neutral protein) and acidic pectin
(rhamnogalacturonan) of all cell wall.
 The major enzymes implicated in the softening of
fruits are hydrolase, pectinase, kinase, amylase etc.
Starch
 During fruit ripening, sugar levels within fruit tend to
increase due to either increased sugar importation from
the plant or to the mobilization of starch reserves within
the fruit, depending on the fruit type and whether it is
ripened on or off the plant.
 With the advancement of maturity, the accumulated
starch is hydrolysed into sugars (glucose, fructose or
sugars) which are known as a characteristic event for
fruit ripening.
 Further breakdown of sucrose into glucose and fructose
is probably mediated by the action of invertase.
 In vegetables like potato and peas on the other hand,
the higher sucrose content which remains high at fresh
immature stage, converts into starch with the approach
of maturity.
Organic Acids

With the onset of fruit ripening there is

downward trend in the levels of organic acids.

The decline in the content of organic acids during

fruit ripening might be the result of an increase in
membrane permeability which allows acids to be
stored in the respiring cells, formation of salts of
malic acid, reduction in the amounts of acid
translocated from the leaves, reduced ability of
fruits to synthesize organic acids with fruit
maturity, translocation into sugars and dilution
effect due to the increase in the volume of fruit.
Colour
The most obvious change which take place is the
degradation of chlorophyll and is accompanied by
the synthesis of other pigments usually either
anthocyanins or carotenoids.
They can give rise to a wide range of colours (from
red to blue).
The chloroplasts in green immature fruit generally
lose chlorophyll on ripening and change into
chromoplasts which contain carotenoid pigments.
Carotenoids are normally synthesized in green plant
tissue a major product being 3-carotene. However,
in many fruits additional carotene and lycopene is
synthesized during ripening.
Flavouring Compounds
Although fruit flavour depends on the
complex interaction of sugars, organic
acids, phenolics and volatile compounds
but the characteristic flavour of an
individual fruit or vegetable is derived
from the production of specific flavouring
volatile compounds.
These compounds are mainly esters,
alcohols, aldehydes, acids and ketones.
Ascorbic Acid
L-ascorbic acid (Vitamin C) is the naturally
occurring ascorbic acid in fruits.
A reduced amount of ascorbic acid is noticed in
pome, stone and berry fruits at the time of
harvest.
An increase in ascorbic acid content with the
increase in fruit growth has been and the levels
declined with the advancement of maturity and
onset of fruit ripening in pear, sweet potatoes,
potato, asparagus and okra during the course of
post harvest handling.
Phenolics
The phenolic content of most fruits declines
from high levels during early growth to low
levels when the fruit is considered to be
physiologically mature
Thereafter, susceptible to the induction of
ripening.

Amino Acids and Proteins

Decrease in free amino acid which often reflects
an increase in protein synthesis.
During senescence the level of free amino acids
increases reflecting a breakdown enzymes and
decreased metabolic activity.
Ethylene Production and Respiration
Physiological events responsible to ripening
process are as follows
Ethylene production
Rise in respiration

Ethylene production in climacteric fruits

such as mango, banana, ethylene increase
and causes:
• Rise in respiration
• Rise in temperature
• Rise in activity of hydrolytic enzymes.
 Ethylene is produced from an essential amino acid
-methionine. Following the steps as below:

SAM: S′adenosyl methionine

ACC: 1-Aminocyclopropane-1-carboxylic acid
Rise in respiration
 Respiration is required for releasing energy and the
substrate for synthesis of several organic compounds
required in the ripening process.
 During ripening in climacteric fruits, there is rise in
respiration called climacteric. The climacteric peak is
obtained very fast when temperature is relatively high.
 Respiration is a most deteriorating process of the harvested
fruits and vegetables which leads to the oxidative
breakdown of the complex materials (carbohydrates or
acids) of cell into simpler molecules (CO2 and water) with
the concurrent production of energy required by the cell for
the completion of chemical reactions.
 In brief, the process of respiration can be summed up with
the following reaction: C6H1206 +6O2 6 CO2 + 6 H20 +
energy
 Different Ripening Methods
 Calcium carbide (CaC2): widely used chemical to
hasten the ripening (release acetylene gas)
 Contains traces of arsenic and phosphorus (toxic
and hazardous to health, believed to affect the
nervous system by reducing the supply of oxygen to
the brain)
 It is banned under 44-AA of PFA Rules, 1955.
 Different Ripening Methods
 Ripening with Artificial ethylene
 Safe method
 Natural gaseous plant hormone emitted by
fruits as ripening initiates.
 Commercial form: Ethrel or ethaphon (2-
chloroethane phosphonic acid)
 Use of ethylene to promote ripening is permitted
under FDA Regulation 120, 1016.
• Application of ethephon promotes degreening
and early ripening in grape, tomato, coffee, pear,
plum, peach and citrus.
• Smoking is commercially employed to hasten de-
greening and ripening of banana and mango.
De-greening in citrus: Carried out in ripening room and this
process takes 2-3 days.
Ventilate daily to ensure that CO2 level does not exceed to 1%
The most rapid de-greening occurs at temperature between 25-
30°c but the best colour development occurs at 15-25°c.
De-greening improves external skin colour and market
acceptance.
Artificial ripening of Tomatoes
How to minimize ethylene production and increase the shelf life????
 Temperature regulation
 With increase in temperature, rate of ripening
increases
 Storage at low temperature reduce the rate of respiration
and ethylene production.
 Storing at low temperature below the optimum level results in
cold injury and decrease in fruit quality. For example, mango is
stored at 8-13°c
Regulation of storage atmosphere
 Natural atmospheric air is conductive for the ethylene
synthesis.
 Decreasing O2 concentration below 5% and increasing CO2
concentration between 3 to 10% delayed ripening by inhibiting
ethylene.

Use of Chemicals for Increasing Shelf Life of Fruits and Vegetables
1. Ethylene absorbent: Ethylene is responsible for decreasing
shelf life. KMnO4 is a good ethylene absorbent. Putting KMnO4
@ 100 ppm soaked filter paper can minimized ripening and
increase shelf life. In Banana, this method is very useful.
2. Antifungal Agents
• SOPP: Sodium orthophenylphenate
• Diphenyl wraps provide protection against moulds, stem-end
rot.
• Dibromoletrachloroethane and esters give better flavour.
3. Use of Inhibitors
Treatment Crop Chemical Concentration
Post-harvest Mango MH 1000-2000 ppm
After fruit Apple 2-Dimethyl- 10,000 ppm
formation hydrazide
4. Use of Auxins: helpful to advance in ripening
and may increase shelf life.
Chemical Concentration Crop Stage

2,4-D 5 ppm Grape Pre-harvest

2,4,5-T 25 ppm Fig Pre-harvest
2,4,5-T 100 ppm Mango After harvesting

5. Vegetables can be preserved by lactic acid and

may increase the shelf life.
6. Post harvest dipping of papaya fruits either in l00
ppm GA3 or CaCl2 al 2% extended shelf life up to 9
days without any decline in quality.