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Chapter 10 Section 4: Andrew Burtsfield

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Chapter 10 section 4

Andrew Burtsfield
Flow of genetic information
 Ribonucleic acid, or RNA acts as an intermediate for
directing the making of proteins
 During transcription, DNA acts as a template for the
synthesis of RNA. In translation, RNA directs the assembly
of proteins
 Forming proteins based on information in DNA and carried
out by RNA is called protein synthesis, or gene expression
RNA STRUCTURE AND
FUNCTION
 RNA contains the sugar ribose, not the sugar deoxyribose
found in DNA
 RNA contains uracil instead of the nitrogenous base thymine
found in DNA
 RNA is usually single-stranded, though some regions may
fold to form short double-stranded sections
 RNA is usually much shorter than length in DNA (about
length of one gene)
TYPES OF RNA
 Cells have three major types of RNA. Each type plays a
different role in protein synthesis.
 The first type is messenger RNA (mRNA), a single-stranded
RNA molecule that carries the instructions from a gene to
make a protein
 The second type is ribosomal RNA (rRNA), which is part of
the structure of ribosomes
 The third type of RNA is transfer RNA (tRNA), which
transfers amino acids to the ribosome to make a protein
TRANSCRIPTION
 Transcription is the process by which the genetic instructions in a
specific gene are transcribed or “rewritten” into an RNA molecule.

 STEPS OF TRANSCRIPTION

 (1) RNA polymerase, an enzyme thatcatalyzes the formation of RNA on


a DNA template, binds to a promoter

 (2) RNA polymerase adds free nucleotides that are complementary to the
nucleotides on one of the DNA strands

 RNA polymerase reaches a termination signal, a specific sequence of


nucleotides that marks the end of a gene. The RNA polymerase now
releases both the DNA and the newly formed RNA.
TRANSCRIPTION
The genetic code is the term for the rules that relate
how a sequence of nitrogenous bases in the
nucleotides corresponds to a particular amino acid
Each three-nucleotide sequence in mRNA that
encodes an amino acid or signifies a start or stop
signal is called a codon
TRANSLATION
 Every protein is made of one or more polypeptides
 They are chains of amino acids linked by peptide bonds
 Steps of Translation
 Two ribosomal units, tRNA and an mRNA, join together.
Enzymes first attach a specific amino acid to one end of each
tRNA according to the genetic code. The other end of each
tRNA contains the anticodon, three nucleotides on the RNA
that are complementary to the sequence of a codon in mRNA
Steps of Translation

 (2) The polypeptide chain is put together. A tRNA carrying


the appropriate amino acid pairs its anticodon with the
second codon in the mRNA. The ribosome then detaches
methionine from the first tRNA, and a peptide bond forms
between methionine and the second amino acid
 (3) The polypeptide chain continues to grow as the mRNA
moves along the ribosome. A new tRNA moves in, carrying
an amino acid for the next mRNA codon
Steps of Translation

 (4) The ribosome reaches the stop codon, and the newly
made polypeptide falls off.
 (5) The components of translation come apart. The last tRNA
leaves the ribosome, and the ribosome move away from the
mRNA
Human Genome
 Genome, the complete genetic content
 An important new field called bioinformatics uses computers
to compared different DNA sequences.
 This is to learn what information the DNA sequences
actually encode

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