Sample Size Calculation
Sample Size Calculation
Sample Size Calculation
CALCULATION
Must remember:
• smaller margin of error means larger sample size
•Higher confidence level means larger sample size
EXAMPLE CALCULATION
A health department nutritionist, wishing to conduct a
survey among a population of teenage girls to
determine the average daily protein intake
What information is needed to estimate the sample
size?
The nutritionist must provide three items of
information: the desired width of the confidence
interval, the level of confidence desired and the
magnitude of the population variance.
WHAT SAMPLE SIZE DO I NEED IF …?
Solution: the nutritionist would like an interval about
10 unit wide; that is, the estimate should be within
about 5 unit of the true value in either direction. A
confidence co-efficient of 95 is decided and on that,
from past experience, the nutritionist feels that the
population standard deviation is probably about 20
grams.
Summarizing the information: z=1.96, p=20,e= 5
n= (1.96)² 20(1-20) = 3.84×380 = 1459.9 = 58.39s
5² 25 25
2. sample size for experimental study
in experimental study where testing the hypothesis that
the difference between two groups, such as
experimental group and control group is significant or
not , the following formula my be used:
n= (α₁+α₂)² (Z₁α⁄₂+Z₁‐β)
(m₁-m₂)²
where,
Z₁α⁄₂: the level of significance for two tails, when
researcher considers that intervention may have
positive or negative effect on outcome variable.
Otherwise, one tail effect is considered only in one
direction (Z₁α)
Z₁‐β : the power researcher considers for
detecting the difference, which is generally
considered 80% or 90%. Z₀.₉₀= 1.28 from
Gaussian table.
σ1=SD of the outcome variable in group -1.
σ2= SD of the outcome variable in group-2.
m₁= mean of the outcome variable in group-1
m₂= mean of the outcome variable in group-2
For example:
A researcher wants to conduct an experiential study to
assess the effect of lowering sodium in diet on systolic
blood pressure (SBP) among hypertensive patients
visiting OPD of AIIMS, Rishikesh.
based on similar research conducted at a different
place or a pilot study, the standard deviation of SBP in
group with low sodium diet is 11.3 mmHg. If α=0.05
and β= 0.10, how many persons from each group
should be studied, if one wants to detect 3.0 mmHg
difference in SBP in the two groups.
to estimate the sample size, researcher will
consider to generalize the findings.
n= (α₁+α₂)² (Z₁α⁄₂+Z₁‐β)
(m₁-m₂)²
Z₁α⁄₂: the level of significance, which is considered as
0.05 for two trails, because researcher considers that
salt intake increase or decrease the SBP. The
Z₀.₉₅=1.96.
Z₁‐β : the power researcher considers for detecting the
difference, which is considered as 0.10 in this study.
Z₀.₉₀= 1.28 from Gaussian table.
σ1=SD of the outcome variable in group -1=11.3 mmHg
σ2= SD of the outcome variable in group-2=13.0 mmHg
m₁-m₂= mean difference of the outcome variable in
group-1 and group-2 =3.0
n= (11.3+13.0)² (1.96+1.28)² =347
(3.0)²
Therefore, a minimum of 347 subjects in each
group would be required to be included in
this study. This calculation assumes the
subjects must be randomized between the
two group. There may not be any non
response in such kind of trials. Other
consideration will be as per mentioned in
example of estimating sample size for
descriptive study.
SAMPLE SIZE CALCULATION
USING COMPUTER
The beginner health care professionals may
find the sample size calculation using
different formulas. For example, they may
use the user friendly, free webpage called
RAOSOFT
http://www.raosoft.com/samplesize.html
BIBLIOGRAPHY