The document provides details about the mass production of bioagents and biopesticides done by a student group in the Entomology Department. The group followed a weekly schedule that included media preparation, inoculation, packaging, and cleaning. They prepared media, grew cultures of Metarhizium, and produced and packaged over 800kg of biopesticide powder. The group also engaged in other activities like rearing insects and preparing artificial diets.
The document provides details about the mass production of bioagents and biopesticides done by a student group in the Entomology Department. The group followed a weekly schedule that included media preparation, inoculation, packaging, and cleaning. They prepared media, grew cultures of Metarhizium, and produced and packaged over 800kg of biopesticide powder. The group also engaged in other activities like rearing insects and preparing artificial diets.
The document provides details about the mass production of bioagents and biopesticides done by a student group in the Entomology Department. The group followed a weekly schedule that included media preparation, inoculation, packaging, and cleaning. They prepared media, grew cultures of Metarhizium, and produced and packaged over 800kg of biopesticide powder. The group also engaged in other activities like rearing insects and preparing artificial diets.
The document provides details about the mass production of bioagents and biopesticides done by a student group in the Entomology Department. The group followed a weekly schedule that included media preparation, inoculation, packaging, and cleaning. They prepared media, grew cultures of Metarhizium, and produced and packaged over 800kg of biopesticide powder. The group also engaged in other activities like rearing insects and preparing artificial diets.
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R.C.S.M.
College of Agriculture, Kolhapur • Name – Savant Aniket Anil • Reg no – K-19/173 • Sem – VII/VIII( NEW) • Course no –SRP-EL ENTO-406 • Course Title – Mass production of • Bioagents and Biopesticides. Actual work Done Entomology department [Mass Production Of Bioagent And Biopesticide] For daily work our module incharge Dr. A.S. Bagade sir made our groups. Group no:- A2 & Group Member :- 5 1. Miss.Kamble Sneha Krushna 2. Miss.Markad Tejaswini Vilas 3. Miss.Kolekar Manasi Kishor 4. Miss.Lomate Pragati Manohar 5. Mr.Kshirsagar Vishal Dattatray.
Group Photo Introduction • As we are allotted the “Entomology Department” for the module, we come to know about different labs of the department. • A lot things we learn starting from, what is biopesticide , how media is prepared , how culture is grown, Metarhizium preparation, packaging and sealing of biopesticide during the course of time . • Here some of the things I am glad to share. Weekly Schedule
Tuesday Wednesday Thursday
Cleaning Mixing & packaging inoculation
Sunday Friday Saturday cleaning Media preparation Selling & marketing Media preparation On Monday we prepare media by following procedure : Material Used: Distilled Water, Autoclave, Jaggery ,Grinder, flasks, plastic container , Cotton plugs, Rubber bands, Paper etc. 1.Take about 1kgof Jaggery for 5 Lit media.(7 Lit of distilled water + 1kg of Jaggery ) 2.Mix it by using Grinder. 3.Fill the clean glass bottles with 100 ml media in each. 4.Cover it with cotton plug and tied with paper and rubber . 5.Autoclaved the bottles at 121°C ,15psi. 5 lit Distilled water 1kg of Jaggery Griend it
Autoclaved the bottle
Filling of glass bottle Plug the bottle Cover with paper Inoculation • On Thursday we do the inoculation by using following procedure. 1.The autoclaved bottles are allowed to cool down and then put it on the table . 2.Switched on UV. Light of the laminar air flow cabinet 20 minutes prior of actual work. 3. After that switched off the U.V. Light and switch on the blower and day light. 4. The working platform of laminar air flow cabinet and hands also disinfected with 0.1%HgCl 2 (mercury chloride) solution. 5. The inoculating needle also flame sterilized then used for inoculation purpose. 6. Then autoclaved media is inoculated with mother culture of Metarhizium anisopliae with the help of sterilized needle. And covered with cotton plug. 7. Bottles are kept on the racks for growth of culture horizontal. The culture become ready for use when it turns Greenish. Laminar air flow Cleaned the laminar air Selecting Mother Culture flow (HgCl2 )
Bottle Rack
Inoculation Metarhizium preparation • On Wednesday in the morning session, we prepared Metarhizium powder by following procedure: 1.First we collect the mature culture in bucket along with media i.e. jaggery . 2.Then grind it well with help of grinder. 3.The grinded material is then well mixed with talcum powder. 4.The mixture is then sieved twice with the help of sieves to make it homogenous. 5.The sieved powder is then weighed on weighing balance and packed in packets of 1 Kg. each. 6.Then it is sealed with help of packaging machine & stored in storage Collection of Collection of Mixing Of Culture With Talcum Powder Grinding Mother Culture Talcum Powder
Sieving Weight Packaging Storage
Lab cleaning
• On every Tuesday & Sunday in the morning session ,
we have to clean inoculation room, store room ,EPN laboratory and porch. • Also washes the glass bottles, flasks , petri plates and other laboratory equipment’s that are to be used in lab. Cleaning of lab Cleaning of glass bottle Cost Of Production • Total Metarhizium Production-3940kg • Batch – A2 Metarhizium production - 858 kg. Activities :- P. D. A. Preparation • Ingredients: Potato : 250 g. Dextrose: 20 g. Agar-Agar: 20 g. Distilled water:1000 ml • Procedure : 1.Wash 250 g. of potato , peel of the skin and slice them into small pieces. 2. Cook the sliced potatoes in 500mL of water in open vessel for 30 minutes. 3.Simultaneously mix 20 g. of agar in 500 mL of distilled water and boil for 30 minutes. 4. Collect the extract by filter through muslin cloth . 5. Add 20 g. Of dextrose to potato extract. 6. Mix thoroughly the molten agar with potato – dextrose mixture and make up the volume to 1 li with distilled water 7.The media then autoclaved at 15 psi Serial dilution • Procedure: 1.Take 10 ml of distilled water in one test tube and 9 ml each in remaining 9 test tubes. It will be 10^-1 concentration. 2.Add 1g. of Metarhizium powder in first test tube. 3.Shake it well. 4.Then transfer 1ml dilute from first test tube to second . 5.Shake it well. Again transfer 1 ml of dilute in next test tube. Follow the same procedure up to 10th test tube. It will be 10^-10 concentration. Preparation Of Serial Dilution COLONY COUNTING • Transfer 1 ml of dilute from each test tube of concentration 10^-1 to 10^-10 into petri-plates • Add small quantity of PDA in petri-plates • Allow it to solidify • Then tie it with rubber • Keep it in BOD incubator for 72hr in inverted position • After 72hr take colony count Rearing of Corcyra Cephalonica Take the wooden box /plastic box of 45 x 30 x 15 cm and clean it properly. The sorghum or wheat grains should be properly heat sterilised in oven for 30 minutes at 100o C The grains should be sprayed with 0.1% formalin Mill the jowar or wheat grains to make 3-5 pieces of grains of each grain Fill the wooden boxes/plastic box with 1. Crushed jowar or wheat grains - 2.5 Kg 2. Crushed groundnut – 25 g 3. Protein – 2 g 4. Sulphur powder – 1 to 1.5 g 5. Streptocyclin – 0.5 g 6. Corcyra cephalonica eggs – 0.5 cc Filled material should be mixed properly and cover the box with lid kept in iron rack for 40 – 45 days After that moths of Corcyra cephalonica will emerge out Collect the moth in ovi-position cage Grain Sterilization Grinding Streptocyklin Taking of Grains
Added Eggs Formalin Mixing Rearing of Mealy Bugs
Medium sized pumpkins with ridges and grooves with a stalk be selected for mass rearing Clean the pumpkins with distilled water and dip in to 0.1% Bavistin solution The wounds are be plugged by paraffin wax to prevent the infection Keep such red pumpkin in the plastic trays introduce the ovi-sacs of mealy bugs on pumpkins After 2 days such pumpkins be kept into wooden Cages provided with the sliding glass at the front end wire and wire gauge and cloth on the other sides These cages are then placed on working tables / racks in the room Mealy bugs will develop in 30-40 days Procedure for prepared of artificial diet First we weighed gram flour 105g, distilled water – 600 mL agar-agar 12.5 g, ascorbic acid -2 g Sorbic acid -1 g, B complex - 1 tablet. We warmed agar-agar in 300 ml water in a big container Then grinded rest material in 300 ml water. This grinded material mixed in agar-agar solution. This semi solid diet poured in aluminum tray. It allowed to cool. Cut the cube of size 1x1x1 cm approximately. This one cube provided to each larvae for feeding Then we changed the diet and plastic bottles simultaneously of Extra Activities
Maize cultivation Gap filling Weeding
Lawn Making Extra Activities
Beautification Work At Main Building
Institute Visit Entrepreneurship skills acquired:
• Business management skill
• Time management and organization skill • Communication and listening skill • Courage and risk taking • confidence • Ambition • Creativity • Ability to Listen Strength Weakness 1 .Better utilization of resources 1. Regular application required. 2. Infrastructure availability 2. Tedious legal procedure for 3. Proper Guidance. registration 4. Available manpower. 3. Lack of awareness among farmers 5. Customer availability 6. Better teamwork. SWOT Analysis
Opportunity Threats 1. Wide market in Kolhapur. Risk in Entrant in 2. May extend business coverage. marketing Thank You
