Autopsy
Autopsy
Autopsy
By
Dr.
Aishwarya
Autopsy is also called as
- Necropsy
- Postmortem examination.
INTRODUCTI Autopsy is a scientific & systemic study of dead body.
ON Auto- self, Opis- view: to see for oneself.
Necros- dead, Opis- view: them most accurate term for
the investigative dissection of the dead body.
Autopsies are of 3 types:
1. Clinical or pathological autopsy,
2. Medicolegal autopsy,
3. Anatomical autopsy.
Clinical/Pathological/Academic Autopsy-
Consent It is carried out under the laws of State only on the requisition of a
legal authority responsible for the investigation of sudden, suspicious
or unnatural death.
The legal authority is usually a police officer not below the rank of sub-
inspector or an Executive Magistrate.
Anatomical autopsy:
It is usually done on an unclaimed dead body, handed over to anatomy
department by government authority. Here permission must be
obtained from government authority
It is also performed on the voluntarily donated dead body. In this
consent from the legal heirs is essential prior to the dissection.
An anatomist performs this.
It is a postmortem examination without compromising the
integrity of the body, even without collection of sample.
Due to some reason or disease the autopsy of dead body is
not possible then by the help of radiological examination we
can detect the cause of death..
VIRTUAL X-rays, ultrasound, CT scan, MRI.
AUTOPSY
A medicolegal autopsy is thus a special type of postmortem
examination, ordered by the government/legal authorities in
all unnatural deaths, such as homicide, suicide, accident.
Objectives for Medicolegal Autopsy
1. To determine the cause of death
2. To determine the manner of death
MEDICOLEG 3. To estimate time since death
AL 4. To establish identity of deceased when not known
Rules - An inquest report is preliminary investigation to ascertain the matter of fact, the
details of body, presence of any injury etc.
5. Autopsy should be done at authorized center, preferably well-equipped mortuary.
6. The doctor-conducting autopsy should carefully read the inquest report and
requisition along with treatment record, if available.
7. The body should be identified by police accompanying the body
8. No unauthorized person should be allowed to be present at autopsy
9. Video recording – in case of death occurring in custody, video recording should be
done and the video tape should be send to the Human Right Commission by doctor
himself.
10. Visit to scene of crime- Examination of scene of crime, though neglected aspect in
India, is important and may provide evidence or clues to the nature and
circumstances of crime.
Autopsy report consist of following parts
1. Introductory part/ Preamble- in this part name, age,
sex, and residence of deceased is mentioned. The place
from where the body was brought, date and time of
Autopsy examination of dead body, mention of authority ordering
the examination are included.
Report 2. Examination part- it consists of external examination
and internal examination and findings recorded by doctor.
3. Opinion or conclusion- the opinion regarding the cause
of death is given. The opinion is deduced from autopsy
findings.
For conducting autopsy along with well-equipped mortuary,
instruments and chemicals are required.
Requiremen
ts
Procedures vary and depend on the skill and experience of forensic expert.
External Examination
It is the most important part of the medicolegal autopsy procedure as most of the
information gathered at this stage can always help a lot in solving the most mysterious
deaths. This includes examination of:
a. Clothes
b. Stains of mud, blood, urine, stool, etc.
c. Identity
d. Body orifices
e. Finger/toe-nails
I shaped
incision
Commences at a point close to acromial process and
extends down below the breast and then medially across
the xipiod process. A similar incision is made at opposite
side of the body and from xipiod process the incision is
carried downwards in a straight line to the pubis.
Indication- Preferred in females
Advantages: It prevents cutting of the chest skin in midline
Y shaped and also allows detailed study of neck structures.
incision
Commence as a straight incision from sternal notch to pubis is
made. Now this incision is extended from suprasternal notch
to the mid-point of clavicle and then upwards towards the
neck behind ear. Similar incision is made on opposite side..
Indications- preferred when a detailed study of neck structures
required, e.g. asphyxial death due to neck compression.
Advantages- It has a better exposure and allows study of neck
Modified Y structures.
shaped
incision
Methods of
Virchow’s Methods
Rokitansky's Method
Dissecting Letulle’s Method
Organs Ghon’s Method
METHODS OF DISSECTION OF
INTERNAL ORGANS
ROKITANSK
VIRCHOW LETULLE GHON
Y
Organ
dissection Organ Organ
dissection dissection
Advocated by Rudolf Virchow (Father of Pathology)
German pathologist (1821-1902)
Organs are removed separately one by one and
studied individually.
VIRCHOW’S Cranial cavity Thoracic cavity Cervical region
METHOD Abdominal cavity
Advantage: Quick & suitable for beginners
Disadvantage: Causes loss of continuity
Difficult to evaluate inter-relationship b/w organs
Advocated by Carl Rokitansky, German pathologist
(1804-1878)
In situ dissection of organs, combined with en bloc
ROKITANSKY
removal.
Used for forensic autopsy to preserve anatomical
’S METHOD relationships & minimize disruption of evidence
Advantages- in infected bodies (HIV, Hepatitis-B),
considered good in children
Disadvantage: difficult to perform
Advocated by Maurice Letulle, French pathologist en
masse method
All organs are removed en masse & dissected as
LETULLE organ block.
Advantage:
METHOD 1) organs inter-relationships are preserved
2) body can be handed over quickly
Disadvantage: organs difficult to handle
Advocated by Anton Ghon, Austrian pathologist
En block method- removed in functional blocks/ groups
Cervical, thoracic, abdominal, pelvic organs are
removed as 3 blocks.
Neuronal system is removed as another block.
GHON’S Advantage:
METHOD 1) Excellent preservation
2) Handling of organs easier
Disadvantage: inter-relationships is difficult to study, if
disease is extending to all blocks.
The skin, subcutaneous and soft tissues in the neck and
chest are then reflected sideward (Bruising of the thoracic
wall, fracture of ribs, etc. should be noted if present).
Cut along the costochondral junction, and reflect the chest
plate.
Keep hands into pleural cavities, look for blood/fluid
Opening of collection.
Disarticulate the sternoclavicular joints on either side, cut
Thoracic the cartilage of 1st rib and separate the chest plate and
remove it.
Cavity Examine the mediastinum for injuries and other findings
and notice accordingly.
In situ inspection is done before removal of thoracic organs
that includes observation of the atrium and ventricle for air
embolism, distension or collapse of lungs, the chest cavity
for fluid, hemorrhage or pus, pleural adhesions, injuries
including fracture of ribs.
The rectus abdominis muscles are incised upto 5 cm above
the symphysis pubis.
A small nick is made in the fascia to put the left index and
middle fingers with palmar surfaces up, to protect the
underlying structures, and the peritoneum is cut up to the
Opening of xiphoid.
Examinatio Poisoning
Mechanical asphyxia
n of Spine Burns
Criminal abortion
Road traffic accident
Newborn/infant/foetus—infanticide case
Firearm injuries
Exhumation
Examination of skeletal remains.
It may be remembered at this juncture that at times unless these special procedures
are opted, it may render difficulties in compiling final opinion in the postmortem
report.
Viscera should be preserved in cases of:
a. Suspected death due to poisoning
b. Deceased was intoxicated or used to drugs
c. Cause of death could not be found after autopsy
Preservatio d. Accidental death involving driver of a vehicle or machine
n of Viscera operator
e. Death due to burns (if needed)
f. Advanced decomposition*
g. Any case, if requested by the Magistrate
Samples preserved in case of suspected poisoning in living-
i. Vomit- 300 ml (whole, if quantity is less)
ii. Stomach washout- 500 ml
iii. Blood- 10 ml
iv. Urine- 100 ml
Examinatio o Weight
n
o Midpoint of body in relation to sternum and umbilicus.
o Skin—wrinkled or smooth with presence of fat, covered with vernix caseosa.
o Nails—appeared or not. If present–extent of growth.
o Scalp hairs—appeared or not.
o Eyelashes and eyebrows—appeared or not.
o Eyelids—adherent or open.
o Testicles—ascertain the position by incising the scrotum and inguinal canal if
necessary.
Ossification centres
Caput succedaneum- over the head (scalp) or buttocks.
Examination of head-
The scalp is reflected by coronal incision as in adults.
For opening the skull cap:
1. Beneke’s technique- dotted line indicate incision
Internal 2. Barr's technique- dotted line indicate incision
Examinatio
n
Abdomen is open first to note the position of diaphragm and
then chest is opened.
In fetal autopsy, technique described by Letulle's is more
beneficial.
Rest part of examination is same as that of adult.
Another technique is outlined by MacPherson and Valdes-
Examinatio Dapera (1991) depending on gestational age of fetus.
HYDROSTATI Procedure
Put each lung separately into a jar of water, see whether it
C TEST sinks or floats.
Cut each into 10 to 12 pieces observe the blood exuding.
Test each of them for buoyancy.
Pick up the floating pieces, and press firmly under water.
Observe the nature of bubbles streaming up. Note whether
they continue to float or not.
Note: Fix a few lung pieces for histopathological examination.
Interpretation
If the entire lung floats- child had not only respired but
respiration had been completely established.
In the absence of putrefaction this test itself is conclusive.
If some pieces float but others sink- child has of course
respired but for a very short period and ineffectively. Yet it is
a live born child.
Interpretati If all pieces sink, the child never respired and is stillborn.
ons Fallacies
Putrefaction: An un respired lung may float due to gases of
decomposition.
Oedema lungs, congenital syphilis, pneumonia, lung
collapse, etc.: These conditions can make a respired lung
sink in water.
But unaffected areas may give a clue to respiration having
been established if that is the case.