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Published November 7, 2017 | Version v1.0
Dataset Open

A Simulated Heterozygous Diploid Genome for Third-gen Sequencing, Assembly, and Curation

  • 1. Australian Wine Research Institute

Description

A simulated heterozygous diploid genome based on Saccharomyces cerevisiae, and S. paradoxus homologous chromosomes.

Simulated PacBio subreads were generated from both parent haplomes and mixed together. A phased assembly was produced using FALCON assembler and FALCON Unzip (doi:10.1038/nmeth.4035). This dataset and assembly were then used to validate the Purge Haplotigs pipeline (https://bitbucket.org/mroachawri/purge_haplotigs). See workflow.sh for commands, comments and file descriptions.

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