The etiology and pathogenesis of polycystic ovary syndrome (PCOS) is still unknown. Using real-ti... more The etiology and pathogenesis of polycystic ovary syndrome (PCOS) is still unknown. Using real-time PCR, we detected that polycystic ovaries showed almost ten times lower expression of ghrelin mRNA than normal ovaries, whereas the mRNA levels in blood cells were similar in both study groups. This suggests that the presence of ghrelin in PCOS and normal ovaries may have an autocrine/paracrine modulatory effect on ovary functions and local significance in the etiology of PCOS.
Ghrelin is one of the peptides involved into GH-release, binding to specific GHS receptors on hyp... more Ghrelin is one of the peptides involved into GH-release, binding to specific GHS receptors on hypothalamus and pituitary. The ghrelin peptide and ghrelin mRNA have been detected in several regions of hypothalamus, in normal pituitary, as well as in various types of pituitary adenoma, with different levels of expression in different tumour types. We decided to determine the expression of ghrelin in somatotroph adenomas. Human pituitary somatotroph adenoma tissues were obtained at the time of transsphenoidal surgery from 3 acromegalic patients and studied for ghrelin mRNA expression. Before surgery each patient received a somatostatin analogue treatment at doses 20 mg, 30 mg, 30 mg at 30 days intervals. 20 mg of each tissue sample was used for the isolation of total cellular RNA. The reverse transcription and real-time PCR were performed according to Korbonits et al. method. The reverse transcription of total RNA to cDNA was performed using Super Script TM Rnase H RT kit according to ...
Cervical cancer in Poland is second most common type of cancer, after breast cancer. There are kn... more Cervical cancer in Poland is second most common type of cancer, after breast cancer. There are known risk factors of cervical cancer development and the most serious one is human papilloma virus infection (HPV). The aim of our paper is present the result study 414 cervical cancer of women from Wielkopolska region treated at the Department of Gynecology and Obstetrics, Karol Marcinkowski University of Medical Sciences in Poznań. In morphological study grading, staging and vascular invasion were estimated. In cervical cancer tissue papillomaviruses PCR method were used. In immunohistochemical study, expression of proteins checking the cell cycle, share in signal transduction to nucleus, cell receptors for steroid hormones and viruses oncogenic proteins were investigated. In the part of cancer gene mutation of p53 (60 cancers) i k-RAS (40 cancers) were searched. In cancers HPV 16/18 infected vascular invasion were more frequently (p < 0.013). No statistically significant difference ...
There are few publication in the literature on DNA HPV 16 in the lymph nodes of cervical cancer p... more There are few publication in the literature on DNA HPV 16 in the lymph nodes of cervical cancer patients. Presence of HPV 16 in the lymph nodes is correlated with metastasis. Our investigation suggests that HPV 16 may be located in the lymph nodes without the presence of cancer cells.
Covalent modifications of histones can regulate all DNA-dependent processes. In the last few year... more Covalent modifications of histones can regulate all DNA-dependent processes. In the last few years, it has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. This review discusses the biology and biochemistry of covalent histone posttranslational modifications (PTMs) and evaluates the dual role of their modifiers in cancer: as oncogenes that can initiate and amplify tumorigenesis or as tumor suppressors.
Plant S1-like nucleases, often referred to as nuclease I enzymes, are the main class of enzymes i... more Plant S1-like nucleases, often referred to as nuclease I enzymes, are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death. The catalytically active site of these enzymes shows a significant similarity to the well-described P1 nuclease from Penicillium citrinum. Previously published studies reported that plant S1-like nucleases possess catalytic activities similar to their fungal orthologs, i.e. they hydrolyze single-stranded DNA and RNA, and less efficiently double-stranded DNA, in the presence of zinc ions. Here we describe a comprehensive study of the nucleolytic activities of all Arabidopsis S1-like paralogs. Our results revealed that different members of this family are characterized by a surprisingly large variety of catalytic properties. We found that, in addition to Zn(2+)-dependent enzymes, this family also comprises nucleases activated by Ca(2+) and Mn(2+), which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Moreover, particular members of this class differ in their optimum pH value and substrate specificity. These results shed new light on the widely accepted classification of plant nucleases which is based on the assumption that the catalytic requirements of plant nucleases reflect their phylogenetic origin. Our results imply the need to redefine the understanding of the term &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;nuclease I&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;. Analysis of the phylogenetic relationships between S1-like enzymes shows that plant representatives of this family evolve toward an increase in catalytic diversity. The importance of this process for the biological functions of plant S1-type enzymes is discussed.
The ability of cells to control the degradation of their own DNA is a common feature of most livi... more The ability of cells to control the degradation of their own DNA is a common feature of most living organisms. In plants, extensive hydrolysis of nuclear DNA occurs during different forms of programmed cell death (PCD). In addition to the removal of unwanted cells, the PCD process allows for the remobilization of cellular constituents, including the products of DNA hydrolysis. Although programmed cell death occurs widely during normal development and plant defense responses to pathogens, only one class of deoxyribonucleases, the S1 type, involved in these processes, has been well characterized. Using DNA-SDS-PAGE, we identified the activities of 14 deoxyribonucleases expressed in different organs of cauliflower seeds, seedlings and the flower head. These enzymes represent several classes based on their substrate specificity and ion dependency. In addition to four Zn(2+)-dependent enzymes, we identified five Ca(2+)-dependent, two Mg(2+)-dependent, three Ca(2+)/Mg(2+)-dependent and one nuclease whose activities seem to be independent of any divalent cations. We also identified a set of DNases whose expression seems to be common for different organs and different stages of development, as well as a few highly tissue-specific nucleases. Expression of three nucleases was inducible by drought stress and hydrogen peroxide.
We have recently identified PARP10 as a novel functional poly(ADP-ribose) polymerase. The gene en... more We have recently identified PARP10 as a novel functional poly(ADP-ribose) polymerase. The gene encoding PARP10 is conserved in vertebrates but no orthologs were found in lower organisms. In addition to the poly(ADP-ribose) polymerase domain, PARP10 possesses several additional sequence motifs, including an RNA recognition motif and two ubiquitin interaction motifs. We characterized the murine genomic locus of the Parp10 gene. We noticed that 3&amp;amp;amp;amp;amp;amp;amp;amp;#39; Parp10 sequences overlapped with the plectin 1 gene in a head-to-tail arrangement. Detailed analyses revealed that the two most 3&amp;amp;amp;amp;amp;amp;amp;amp;#39; Parp10 exons (exons 10 and 11) are also used for plectin 1. While these two exons code for part of the poly(ADP-ribose) polymerase domain in Parp10, they are noncoding for plectin 1 due to the lack of appropriate start codons. Furthermore our findings suggest that at least one of the plectin 1 promoters is located within intron 9 of the Parp10 gene.
The etiology and pathogenesis of polycystic ovary syndrome (PCOS) is still unknown. Using real-ti... more The etiology and pathogenesis of polycystic ovary syndrome (PCOS) is still unknown. Using real-time PCR, we detected that polycystic ovaries showed almost ten times lower expression of ghrelin mRNA than normal ovaries, whereas the mRNA levels in blood cells were similar in both study groups. This suggests that the presence of ghrelin in PCOS and normal ovaries may have an autocrine/paracrine modulatory effect on ovary functions and local significance in the etiology of PCOS.
Ghrelin is one of the peptides involved into GH-release, binding to specific GHS receptors on hyp... more Ghrelin is one of the peptides involved into GH-release, binding to specific GHS receptors on hypothalamus and pituitary. The ghrelin peptide and ghrelin mRNA have been detected in several regions of hypothalamus, in normal pituitary, as well as in various types of pituitary adenoma, with different levels of expression in different tumour types. We decided to determine the expression of ghrelin in somatotroph adenomas. Human pituitary somatotroph adenoma tissues were obtained at the time of transsphenoidal surgery from 3 acromegalic patients and studied for ghrelin mRNA expression. Before surgery each patient received a somatostatin analogue treatment at doses 20 mg, 30 mg, 30 mg at 30 days intervals. 20 mg of each tissue sample was used for the isolation of total cellular RNA. The reverse transcription and real-time PCR were performed according to Korbonits et al. method. The reverse transcription of total RNA to cDNA was performed using Super Script TM Rnase H RT kit according to ...
Cervical cancer in Poland is second most common type of cancer, after breast cancer. There are kn... more Cervical cancer in Poland is second most common type of cancer, after breast cancer. There are known risk factors of cervical cancer development and the most serious one is human papilloma virus infection (HPV). The aim of our paper is present the result study 414 cervical cancer of women from Wielkopolska region treated at the Department of Gynecology and Obstetrics, Karol Marcinkowski University of Medical Sciences in Poznań. In morphological study grading, staging and vascular invasion were estimated. In cervical cancer tissue papillomaviruses PCR method were used. In immunohistochemical study, expression of proteins checking the cell cycle, share in signal transduction to nucleus, cell receptors for steroid hormones and viruses oncogenic proteins were investigated. In the part of cancer gene mutation of p53 (60 cancers) i k-RAS (40 cancers) were searched. In cancers HPV 16/18 infected vascular invasion were more frequently (p < 0.013). No statistically significant difference ...
There are few publication in the literature on DNA HPV 16 in the lymph nodes of cervical cancer p... more There are few publication in the literature on DNA HPV 16 in the lymph nodes of cervical cancer patients. Presence of HPV 16 in the lymph nodes is correlated with metastasis. Our investigation suggests that HPV 16 may be located in the lymph nodes without the presence of cancer cells.
Covalent modifications of histones can regulate all DNA-dependent processes. In the last few year... more Covalent modifications of histones can regulate all DNA-dependent processes. In the last few years, it has become more and more evident that histone modifications are key players in the regulation of chromatin states and dynamics as well as in gene expression. Therefore, histone modifications and the enzymatic machineries that set them are crucial regulators that can control cellular proliferation, differentiation, plasticity, and malignancy processes. This review discusses the biology and biochemistry of covalent histone posttranslational modifications (PTMs) and evaluates the dual role of their modifiers in cancer: as oncogenes that can initiate and amplify tumorigenesis or as tumor suppressors.
Plant S1-like nucleases, often referred to as nuclease I enzymes, are the main class of enzymes i... more Plant S1-like nucleases, often referred to as nuclease I enzymes, are the main class of enzymes involved in nucleic acid degradation during plant programmed cell death. The catalytically active site of these enzymes shows a significant similarity to the well-described P1 nuclease from Penicillium citrinum. Previously published studies reported that plant S1-like nucleases possess catalytic activities similar to their fungal orthologs, i.e. they hydrolyze single-stranded DNA and RNA, and less efficiently double-stranded DNA, in the presence of zinc ions. Here we describe a comprehensive study of the nucleolytic activities of all Arabidopsis S1-like paralogs. Our results revealed that different members of this family are characterized by a surprisingly large variety of catalytic properties. We found that, in addition to Zn(2+)-dependent enzymes, this family also comprises nucleases activated by Ca(2+) and Mn(2+), which implies that the apparently well-known S1 nuclease active site in plant nucleases is able to cooperate with different activatory ions. Moreover, particular members of this class differ in their optimum pH value and substrate specificity. These results shed new light on the widely accepted classification of plant nucleases which is based on the assumption that the catalytic requirements of plant nucleases reflect their phylogenetic origin. Our results imply the need to redefine the understanding of the term &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;nuclease I&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;. Analysis of the phylogenetic relationships between S1-like enzymes shows that plant representatives of this family evolve toward an increase in catalytic diversity. The importance of this process for the biological functions of plant S1-type enzymes is discussed.
The ability of cells to control the degradation of their own DNA is a common feature of most livi... more The ability of cells to control the degradation of their own DNA is a common feature of most living organisms. In plants, extensive hydrolysis of nuclear DNA occurs during different forms of programmed cell death (PCD). In addition to the removal of unwanted cells, the PCD process allows for the remobilization of cellular constituents, including the products of DNA hydrolysis. Although programmed cell death occurs widely during normal development and plant defense responses to pathogens, only one class of deoxyribonucleases, the S1 type, involved in these processes, has been well characterized. Using DNA-SDS-PAGE, we identified the activities of 14 deoxyribonucleases expressed in different organs of cauliflower seeds, seedlings and the flower head. These enzymes represent several classes based on their substrate specificity and ion dependency. In addition to four Zn(2+)-dependent enzymes, we identified five Ca(2+)-dependent, two Mg(2+)-dependent, three Ca(2+)/Mg(2+)-dependent and one nuclease whose activities seem to be independent of any divalent cations. We also identified a set of DNases whose expression seems to be common for different organs and different stages of development, as well as a few highly tissue-specific nucleases. Expression of three nucleases was inducible by drought stress and hydrogen peroxide.
We have recently identified PARP10 as a novel functional poly(ADP-ribose) polymerase. The gene en... more We have recently identified PARP10 as a novel functional poly(ADP-ribose) polymerase. The gene encoding PARP10 is conserved in vertebrates but no orthologs were found in lower organisms. In addition to the poly(ADP-ribose) polymerase domain, PARP10 possesses several additional sequence motifs, including an RNA recognition motif and two ubiquitin interaction motifs. We characterized the murine genomic locus of the Parp10 gene. We noticed that 3&amp;amp;amp;amp;amp;amp;amp;amp;#39; Parp10 sequences overlapped with the plectin 1 gene in a head-to-tail arrangement. Detailed analyses revealed that the two most 3&amp;amp;amp;amp;amp;amp;amp;amp;#39; Parp10 exons (exons 10 and 11) are also used for plectin 1. While these two exons code for part of the poly(ADP-ribose) polymerase domain in Parp10, they are noncoding for plectin 1 due to the lack of appropriate start codons. Furthermore our findings suggest that at least one of the plectin 1 promoters is located within intron 9 of the Parp10 gene.
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Papers by Elzbieta Poreba