Annali d’Italia VOL. 1 ISSN 3572-2436 Annali d’Italia (Italy’s scientific journal) is a peer-revi... more Annali d’Italia VOL. 1 ISSN 3572-2436 Annali d’Italia (Italy’s scientific journal) is a peer-reviewed European journal covering top themes and problems in various fields of science. The journal offers authors the opportunity to make their research accessible to everyone, opening their work to a wider audience. Chief editor: Cecilia Di Giovanni Managing editor: Giorgio Bini
This article is devoted to the development of new environmentally friendly growth regulators of a... more This article is devoted to the development of new environmentally friendly growth regulators of an important technical crop – oilseed flax (Linum usitatissimum L.) of the cultivar Iceberg based on synthetic compounds, derivatives of N-oxide-2,6-dimethylpyridine (Ivin), 6-methyl-2-mercapto-4-hydroxypyrimidine sodium and po-tassium salts (Methyur and Kamethur). The effect of synthetic plant growth regulators Ivin, Methyur and Kamethur used separately or in complex with microfertilizers Rostok Extra and Radix Tim forte+ on the growth of oilseed flax of the cultivar Iceberg during the vegetative period was investigated. The stimulatory effect of synthetic plant growth regulators and microfertilizers on improving the growth indicators of oilseed flax of the cultivar Iceberg under the conditions of their separate use or complex use was established. The activity of the synthetic plant growth regulators Ivin, Methyur and Kamethur under the conditions of their separate use or complex use with microferti-lizers was similar to or exceeded the activity of the phytohormone auxin IAA (1H-indole-3-acetic acid). The high-est values of average length of shoots (cm) and average length of roots (cm) of oilseed flax of the cultivar Iceberg were obtained under conditions of separate use of synthetic plant growth regulators Methyur and Kamethur, or microfertilizers Rostok Extra and Radix Tim forte+, as well as under conditions of complex use of synthetic plant growth regulators with microfertilizers: Ivin+Radix Tim forte+, Methyur+Rostok Extra, Methyur + Radix Tim forte+, Kamethur+Rostok Extra, Kamethur+ Radix Tim forte+.
Keywords: oilseed flax (Linum usitatissimum L.), Ivin, Methyur, Kamethur, Rostok Extra, Radix Tim forte+.
The regulatory effect of synthetic plant growth regulators, pyrimidine and pyridine derivatives: ... more The regulatory effect of synthetic plant growth regulators, pyrimidine and pyridine derivatives: Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives, on growth and photosynthesis of microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered was studied. The growth-regulating effect of synthetic plant growth regulators Methyur, Kamethur, Ivin, as well as new pyrimidine derivatives, was compared with the growth-regulating effect of phytohormones auxins IAA and NAA. The results obtained showed that synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, have both auxin-like and cytokinin-like effect on enhancing the growth of shoots and roots of three-week-old microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. It was also found that the synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, exhibit a cytokinin-like effect on the increasing the content of photosynthetic pigments (chlorophylls a, b, a+b and carotenoids) in the leaves of three-week-old microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. The use of synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic pyrimidine derivatives at a concentration of 10-7M to improve the growth of shoots and the roots of pea microgreens, as well as to increase the content of chlorophylls a, b, a+b and carotenoids in pea microgreens is proposed. Keywords: Pea microgreens; Shoots and Roots, Chlorophylls a, b, a+b and Carotenoids; IAA and NAA; Methyur; Kamethur; Ivin; Pyrimidine and Pyridine Derivatives.
The scientific heritage (Budapest, Kossuth Lajos utca 84,1204, Hungary). The journal is registere... more The scientific heritage (Budapest, Kossuth Lajos utca 84,1204, Hungary). The journal is registered and published in Hungary. The journal publishes scientific studies, reports and reports about achievements in different scientific fields.
The journal is registered and published in Czech Republic (Křižíkova 384/101 Karlín, 186 00 Praha... more The journal is registered and published in Czech Republic (Křižíkova 384/101 Karlín, 186 00 Praha). Articles in all spheres of sciences are published in the journal.
This work is devoted to the screening for new effective plant growth regulators based on syntheti... more This work is devoted to the screening for new effective plant growth regulators based on synthetic compounds, pyrimidine and pyridine derivatives. Study of the plant growth-regulating activity of synthetic compounds was carried out on spring barley (Hordeum vulgare L.) variety Avatar. The effect of synthetic compounds on the morphometric parameters of barley plants grown in laboratory conditions was studied. The plant growth-regulating activity of synthetic compounds was compared with the plant growth-regulating activity of phytohormones auxins IAA (1H-indol-3-yl)acetic acid) and NAA (1-naphthylacetic acid). The most biologically active synthetic compounds were selected, which show plant growth-regulating activity, similar to or exceeding the activity of auxins IAA and NAA. The highest parameters of the average length of the shoots, which exceeded similar parameters of control plants by 13.73-24.06 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of sodium and potassium salts of 6-methyl-2-mercapto-4hydroxypyrimidine (Methyur and Kamethur), 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, 2-benzylsulfanyl-6methylpyrimidin-4-ol, and sodium 4-hydroxypy-rimidine-2-thiolate, respectively. The highest parameters of the average length of the roots, which exceeded similar parameters of control plants by 66.67-121.35 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of N-oxide-2,6dimethylpyridine (Ivin), sodium and potassium salts of 6-methyl -2-mercapto-4-hydroxypyrimidine (Methyur and Kamethur), 2-ethylsulfanyl-6-methylpyrimidin-4-ol, and 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, respectively. The highest parameters of the average biomass of 10 plants, which exceeded similar parameters of control plants by 30.59-86.46 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of N-oxide-2,6-dimethylpyridine (Ivin), sodium and potassium salts of 6-methyl-2-mercapto-4hydroxypyrimidine (Methyur and Kamethur), 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, and sodium 4hydroxypyrimidine-2-thiolate, respectively. A comparative analysis of the growth-regulating activity of auxins showed that the morphometric parameters obtained on 2-week-old barley plants treated with IAA and NAA, exceeded similar parameters of control plants, as follows: plant shoot length by-7.51-20.57 %, plant root lengthby 15.45-32.81 %, plant biomassby 39.59-49.01 %, respectively. It was shown that pyrimidine and pyridine derivatives at a concentration of 10-7 M exhibit cytokinin-like activity in increasing the content of photosynthetic pigments in the leaves of barley plants. The content of chlorophyll a increased by 6.46-68.33%, chlorophyll b increased by 6.2-64.11%, chlorophyll a+b increased by 6.37-66.81%, carotenoids increased by 2.65-29.81%, respectively, compared with similar parameters of control barley plants treated with distilled water. The practical use of the synthetic compounds, pyrimidine and pyridine derivatives: Methyur, Kamethur, Ivin, and other selected most active synthetic compounds for improving the development of shoots and roots, increasing the biomass of spring barley (Hordeum vulgare L.) variety Avatar in the vegetative phase, and increasing the content of photosynthetic pigments in plant leaves is proposed.
Our chapter is devoted to the biological screening of new effective substitutes of plant hormones... more Our chapter is devoted to the biological screening of new effective substitutes of plant hormones auxins and cytokinins among synthetic low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidines, pyrazolotriazinones, oxazoles, oxazolopyrimidines and isoflavonoids. The auxin-like and cytokinin-like activity of chemical low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole was studied. A specific bioassay on auxin-like activity showed a high stimulating effect of the chemical heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole and isoflavones used at the concentration 10–8 M on the formation of adventitious roots on the 14th-day-old leaf petioles isolated from seedlings of haricot bean (Phaseolus vulgaris L.) cultivar Belozernaya, which was similar or higher of the effect of plant hormones auxins IAA and NAA used at the same concentration 10−8 M. A specific bioassay on cytokinin-like activity showed a high stimulating effect of the chemical heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole used at the concentrations 10–8 M and 10–9 M on the growth of biomass of 16th-day-old cotyledons isolated from seeds of muscat pumpkin (Cucurbita moschata Duch. et Poir.) cultivar Gilea, which was similar or higher of the effect of plant hormone cytokinin Kinetin used at the same concentrations 10–8 M and 10–9 M. The results obtained confirmed the inducing auxin-like and cytokinin-like effect of chemical low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole on plant cell elongation, division, and differentiation that are the basic processes of plant growth. The practical application of derivatives of pyrimidine, pyrazole, isoflavones, pyridine, oxazolopyrimidine and oxazole as new plant growth regulators was proposed. Keywords Plant hormones IAA NAA Kinetin Pyridine Pyrimidine Pyrazole Isoflavones Oxazolopyrimidine Oxazole Phaseolus vulgaris Cucurbita moschataDuch. et Poir
Wheat is one of the economically important cereals that are widely used in the agricultural sect... more Wheat is one of the economically important cereals that are widely used in the agricultural sector of many countries. A very important issue is the development of new effective growth regulators to improve the growth and development of this crop in order to increase its productivity. In our investigations the regulating activity of the synthetic low molecular weight heterocyclic compounds, pyrimidine derivatives on growth and development of wheat (Triticum aestivum L.) variety Tyra was studied. It was found that the use of pyrimidine derivatives in a concentration of 10-7M in water solution revealed a positive effect on the growth and development of both shoot and root systems of wheat plants during the vegetation period. Comparative analysis of the growth regulating activity of synthetic compounds showed that the activity of pyrimidine derivatives was differentiated and depended on substituents in their chemical structure. The application of pyrimidine derivatives as new effective...
The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of... more The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of new effective and ecologically safe plant growth regulating substances on the base of synthetic low molecular weight five and six-membered heterocyclic compounds derivatives of pyridine, pyrimidine, pyrazole, oxazole, and isoflavones as an effective substitutes of phytohormones and traditional plant growth regulators to develop agricultural crops with genetically improved commercially important traits such as accelerated growth and development, increased plant productivity and improved quality of production, and enhanced plant tolerance to abiotic stress factors such as drought, salinity, heat, chilling, osmotic stress, soil pollution by anthropogenic factors, and to biotic stress factors such as pathogens and pests [1]. Maize (Zea mays L.) is ancient cereal crop traditionally used as source for dietary food and pharmaceutical industry due to high content of nutrients such as lipids, proteins, carbohydrates, vitamins, dietary fiber and biological active compounds in the seed and seed oil, and as raw material for the biofuel industry and animal feed [2]. Our work was aimed to study of plant growth regulating activity of new synthetic low molecular weight heterocyclic compounds phosphorylated derivatives of oxazole and pyrimidine with perspective their application as a new effective substitutes of phytohormones for improving of cultivation of maize (Zea mays L.) hybrid Palmyra FAO 190. The conducted in the laboratory conditions researches showed high growth stimulating auxin-like effect of tested chemical compounds on germination of seeds, growth and development of maize seedlings during the vegetation. The comparative statistical analysis showed that the biometric indexes of 14 th-day-old seedlings of maize grown on the water solution of heterocyclic compounds used at concentration 10-9 M were higher than the biometric indexes of 21 st-day-old seedlings of maize grown either on the distilled water (control) or on the water solution of auxins IAA and NAA used at the same concentration 10-9 M in average: at the 23 %-by total length of seedlings, 5-37 %-by total length of roots, and at the 7-87 %-by total number of roots. The positive effect of some from tested chemical heterocyclic compounds on increase of synthesis of photosynthetic pigments in the leaves of 14 th-day-old seedlings of maize was also found: the content of chlorophyll a was increased in average at the 5-10 %; the content of chlorophyll b was increased in average at the 10-74 %; the content of chlorophylls a+b was increased in average at the 5-11 %. The practical application in the agricultural biotechnology of synthetic low molecular weight heterocyclic compounds phosphorylated derivatives of oxazole and pyrimidine as new effective substitutes of phytohormones for improving of growth and development of maize (Zea mays L.) hybrid Palmyra FAO 190 was proposed. 1. V. Tsygankova, Y. Andrusevich, O. Shtompel, O. Myroljubov, A. Hurenko, R. Solomyanny, G. Mrug, M. Frasinyuk, O. Shablykin, V. Brovarets. Study of Auxin, Cytokinin and Gibberellin-like Activity of Heterocyclic Compounds Derivatives of Pyrimidine, Pyridine, Pyrazole and Isoflavones. European Journal of Biotechnology and Bioscience, 2016, 4(12): 29-44. 2. Shah T.R., Prasad K., Kumar P. Maize-A potential source of human nutrition and health: A review. Cogent Food & Agriculture. 2016, 2: 1166995.
The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of... more The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of new effective and ecologically safe plant growth regulating substances on the base of synthetic low molecular weight five and six-membered heterocyclic compounds derivatives of pyridine, pyrimidine, pyrazole, oxazole, and isoflavones as an effective substitutes of phytohormones and traditional plant growth regulators to develop agricultural crops with genetically improved commercially important traits such as accelerated growth and development, increased plant productivity and improved quality of production, and enhanced plant tolerance to abiotic stress factors such as drought, salinity, heat, chilling, osmotic stress, soil pollution by anthropogenic factors, and to biotic stress factors such as pathogens and pests [1-3]. Tomato is a commercially important food crop in the world. Tomato is a widely used crop, moreover, it is a source of carotenoids and flavonoids [4]. The protective properties of carotenoids are mainly due to their high antioxidant activity, as well as the antioxidant activity attributed to lycopene, which is an acyclic isomer of beta-carotene and is abundantly contained in red tomatoes [5]. Unlike beta-carotene, lycopene can not be metabolized to vitamin A in the human body, but it has a dual antioxidant potential of beta-carotene. The using of tomato and tomato products that contain lycopene reduces the risk of developing chronic diseases, such as cancer, cardiovascular and some age-related diseases [6]. This work is aimed to study of stimulating effect of new synthetic low molecular weight compounds derivatives of pyrimidine, on the growth and development of tomato (Solanum lycopersicum L.) plants under laboratory conditions. Key words: tomato (Solanum lycopersicum L.), auxins IAA and NAA, cytokinin Kinetin, synthetic low molecular weight heterocyclic compounds, pyrimidine, plant growth regulating activity, agricultural biotechnology.
Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used i... more Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used in biotechnology as a source for bioactive compounds. Flax fiber and seed have industrial significance as a source of cellulosic fiber for textile and paper industry and seed oil for pharmaceutical, cosmetic and food industry. The elaboration of new effective techniques for in vitro regeneration of Linum usitatissimum L. is very actual problem today. The influence of low molecular weight five and six membered heterocyclic compounds on organogenesis of Linum usitatissimum L. cultivar heavenly in vitro has been studied. It was found that heterocyclic compounds derivatives pyridine, pyrimidine, pyrazole and isoflavones revealed high stimulating effect on direct shoot organogenesis of Linum usitatissimum L. in vitro. The maximal shoot regeneration frequency was obtained on the MS media containing heterocyclic compounds at the concentration 10-8 M/l as compared to lower regeneration frequency obtained on the control MS medium containing 1 mg/l BAP and 0.05 mg/l NAA. The maximal index of explants with regeneration (in %) that exceeded almost twice the same index in control MS medium was obtained on the MS medium containing heterocyclic compound derivative of isoflavones. At the same time, the index of explants with regeneration (in %) that exceeded one and a half times the same index in control MS medium and the maximal index of shoots elongated more than 1 cm (in %) that exceeded almost twice the same index in control MS medium were obtained on the MS media containing heterocyclic compounds derivatives of pyridine, pyrimidine and pyrazole. The current study confirms perspective of using of low molecular weight five and six-membered heterocyclic compounds at the concentration 10-8 М/l of the MS medium as new effective substitutes of traditional growth regulators auxin NAA and cytokinin BAP for regeneration of Linum usitatissimum L. cultivar heavenly in vitro. Keywords: screening of new plant growth regulators, nitrogen-containing heterocyclic compounds, pyridine, pyrimidine, pyrazolotriazinone and isoflavones, Linum usitatissimum L., direct shoot organogenesis in vitro
Application of Oxazole and Oxazolopyrimidine as New Effective Regulators of Oilseed Rape Growth V... more Application of Oxazole and Oxazolopyrimidine as New Effective Regulators of Oilseed Rape Growth Victoria Tsygankova*, Yaroslav Andrusevich, Victor Kopich, Olexandra Shtompel, Yevheniia Veligina, Stepan Pilyo, Maryna Kachaeva, Andrii Kornienko, Volodymyr Brovarets Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 1, Murmanskaya str., Kyiv, Ukraine.
Tomato (Solanum lycopersicum L.) is a major agricultural crop cultivated over the world. The elab... more Tomato (Solanum lycopersicum L.) is a major agricultural crop cultivated over the world. The elaboration of new effective plant growth regulators for improving growth of tomato is an important task for plant biotechnology. In the present work the comparative effect of plant hormones auxins IAA and NAA, cytokinin Kinetin, and new synthetic compounds pyrimidine derivatives on acceleration of vegetative growth of tomato was studied. The obtained biometric indices of 24 th-day-old tomato seedlings grown on the water solution of pyrimidine derivatives used at the concentration 10-8 M were similar or higher of the biometric indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin used at the same concentration 10-8 M in average: at the 29-255 %, 22-235 %, 10-156 %, and 13-95 %-by total number of roots, and at the 23-255 %, 21-115 %, 19-138 %, and 9-61 %-by total length of roots, respectively. The content of photosynthetic pigments in tomato seedlings grown on the water solution of pyrimidine derivatives was increased in average: chlorophyll a-at the 17-116 %, 17-122 %, 19-22 %, and 19-52 %; chlorophyll bat the 19-155 %, 21-161 %, 12-45 %, and 11-81 %; chlorophylls a+b-at the 16-126 %, 13-132 %, 23-28 %, and 14-60 %; carotenoids-at the 15-86 %, 19-107 %, 20-31%, and 16-50 %, as compared with similar indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin, respectively. The content of total soluble proteins in tomato seedlings grown on the water solution of pyrimidine derivatives was increased in average: at the 40-241 %, 9-145 %, 15-122 %, and 17-123 %, as compared with similar indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin, respectively. The relationship between chemical structure and plant growth regulatory activity of pyrimidine derivatives was set. The highest growth regulatory activity revealed the compounds, derivatives of dihydroimidazo[1,2-c]pyrimidine. The obtained results confirmed the possibility of application of pyrimidine derivatives as new effective regulators of vegetative growth of tomato.
European Journal of Biotechnology and Bioscience, 2016
Impact of microbial biostimulants on the increase (up to 75-87%) of resistance of wheat plants of... more Impact of microbial biostimulants on the increase (up to 75-87%) of resistance of wheat plants of two varieties Yatran 60 and Zimoyarka to the cereal cyst nematode Heterodera avenae was found. The increase of difference in the index of Dot-blot hybridization (up to 37-65%) between cytoplasmic mRNA and si/miRNA isolated from control and experimental (affected by nematode and treated with biostimulants) plants was observed. In the wheat embryo cell-free system the increase of silencing activity (up to 48-78%) of si/miRNA isolated from experimental as compared to control wheat plants was revealed. These data indicate that microbial biostimulants cause reprogramming wheat genome by inducing synthesis si/miRNA with immuneprotective properties against nematode; as a result the wheat resistance to nematode invasion is increased. To obtain new lines of wheat cells resistant to nematode H. avenae the microbial biostimulants were used for preparing of MS media for cultivation of isolated whea...
This book covers all areas of biotechnology, genetics and other related fields. The contributions... more This book covers all areas of biotechnology, genetics and other related fields. The contributions by the authors include biosurfactants; antimicrobial activity; bio-preservation; shelf life; plasmid; algal plasmid; plasmid like DNA; fungal contamination; mycotoxins; Detarium microcarpum; Brachystegia eurycoma; Huanglongbing disease; Candidatus liberibacter asiaticus; citrus; Jatropha curcas; euphorbiaceae; essential oil; palmitic acid; linoleic acid; alloisolongifolene; β-farnesene; moisture barrier; shelf-life of horticultural produce; SGOT; SGPT; alloxan monohydrate; aspirin; membrane stabilization; cellulase activity; actinomycetes; fermentation; carboxymethyl cellulose; xylanolytic activity; Trametes sp.; Annona senegalensis; Oulotricha subspecies; thermophiles; bacterial isolates; antibiotic resistance; pathogenicity; urinary tract infections; Dental Pulp Stem Cells (DPSC); Bone Morphogenic Protein (BMP-2) etc. This book contains various materials suitable for students, researchers and academicians in the field of biotechnology and genetics. Key words: Biotechnology, Genetics
Annali d’Italia VOL. 1 ISSN 3572-2436 Annali d’Italia (Italy’s scientific journal) is a peer-revi... more Annali d’Italia VOL. 1 ISSN 3572-2436 Annali d’Italia (Italy’s scientific journal) is a peer-reviewed European journal covering top themes and problems in various fields of science. The journal offers authors the opportunity to make their research accessible to everyone, opening their work to a wider audience. Chief editor: Cecilia Di Giovanni Managing editor: Giorgio Bini
This article is devoted to the development of new environmentally friendly growth regulators of a... more This article is devoted to the development of new environmentally friendly growth regulators of an important technical crop – oilseed flax (Linum usitatissimum L.) of the cultivar Iceberg based on synthetic compounds, derivatives of N-oxide-2,6-dimethylpyridine (Ivin), 6-methyl-2-mercapto-4-hydroxypyrimidine sodium and po-tassium salts (Methyur and Kamethur). The effect of synthetic plant growth regulators Ivin, Methyur and Kamethur used separately or in complex with microfertilizers Rostok Extra and Radix Tim forte+ on the growth of oilseed flax of the cultivar Iceberg during the vegetative period was investigated. The stimulatory effect of synthetic plant growth regulators and microfertilizers on improving the growth indicators of oilseed flax of the cultivar Iceberg under the conditions of their separate use or complex use was established. The activity of the synthetic plant growth regulators Ivin, Methyur and Kamethur under the conditions of their separate use or complex use with microferti-lizers was similar to or exceeded the activity of the phytohormone auxin IAA (1H-indole-3-acetic acid). The high-est values of average length of shoots (cm) and average length of roots (cm) of oilseed flax of the cultivar Iceberg were obtained under conditions of separate use of synthetic plant growth regulators Methyur and Kamethur, or microfertilizers Rostok Extra and Radix Tim forte+, as well as under conditions of complex use of synthetic plant growth regulators with microfertilizers: Ivin+Radix Tim forte+, Methyur+Rostok Extra, Methyur + Radix Tim forte+, Kamethur+Rostok Extra, Kamethur+ Radix Tim forte+.
Keywords: oilseed flax (Linum usitatissimum L.), Ivin, Methyur, Kamethur, Rostok Extra, Radix Tim forte+.
The regulatory effect of synthetic plant growth regulators, pyrimidine and pyridine derivatives: ... more The regulatory effect of synthetic plant growth regulators, pyrimidine and pyridine derivatives: Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives, on growth and photosynthesis of microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered was studied. The growth-regulating effect of synthetic plant growth regulators Methyur, Kamethur, Ivin, as well as new pyrimidine derivatives, was compared with the growth-regulating effect of phytohormones auxins IAA and NAA. The results obtained showed that synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, have both auxin-like and cytokinin-like effect on enhancing the growth of shoots and roots of three-week-old microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. It was also found that the synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, exhibit a cytokinin-like effect on the increasing the content of photosynthetic pigments (chlorophylls a, b, a+b and carotenoids) in the leaves of three-week-old microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. The use of synthetic plant growth regulators Methyur, Kamethur and Ivin, as well as new synthetic pyrimidine derivatives at a concentration of 10-7M to improve the growth of shoots and the roots of pea microgreens, as well as to increase the content of chlorophylls a, b, a+b and carotenoids in pea microgreens is proposed. Keywords: Pea microgreens; Shoots and Roots, Chlorophylls a, b, a+b and Carotenoids; IAA and NAA; Methyur; Kamethur; Ivin; Pyrimidine and Pyridine Derivatives.
The scientific heritage (Budapest, Kossuth Lajos utca 84,1204, Hungary). The journal is registere... more The scientific heritage (Budapest, Kossuth Lajos utca 84,1204, Hungary). The journal is registered and published in Hungary. The journal publishes scientific studies, reports and reports about achievements in different scientific fields.
The journal is registered and published in Czech Republic (Křižíkova 384/101 Karlín, 186 00 Praha... more The journal is registered and published in Czech Republic (Křižíkova 384/101 Karlín, 186 00 Praha). Articles in all spheres of sciences are published in the journal.
This work is devoted to the screening for new effective plant growth regulators based on syntheti... more This work is devoted to the screening for new effective plant growth regulators based on synthetic compounds, pyrimidine and pyridine derivatives. Study of the plant growth-regulating activity of synthetic compounds was carried out on spring barley (Hordeum vulgare L.) variety Avatar. The effect of synthetic compounds on the morphometric parameters of barley plants grown in laboratory conditions was studied. The plant growth-regulating activity of synthetic compounds was compared with the plant growth-regulating activity of phytohormones auxins IAA (1H-indol-3-yl)acetic acid) and NAA (1-naphthylacetic acid). The most biologically active synthetic compounds were selected, which show plant growth-regulating activity, similar to or exceeding the activity of auxins IAA and NAA. The highest parameters of the average length of the shoots, which exceeded similar parameters of control plants by 13.73-24.06 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of sodium and potassium salts of 6-methyl-2-mercapto-4hydroxypyrimidine (Methyur and Kamethur), 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, 2-benzylsulfanyl-6methylpyrimidin-4-ol, and sodium 4-hydroxypy-rimidine-2-thiolate, respectively. The highest parameters of the average length of the roots, which exceeded similar parameters of control plants by 66.67-121.35 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of N-oxide-2,6dimethylpyridine (Ivin), sodium and potassium salts of 6-methyl -2-mercapto-4-hydroxypyrimidine (Methyur and Kamethur), 2-ethylsulfanyl-6-methylpyrimidin-4-ol, and 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, respectively. The highest parameters of the average biomass of 10 plants, which exceeded similar parameters of control plants by 30.59-86.46 %, were obtained on 2-week-old barley plants treated with synthetic compounds, derivatives of N-oxide-2,6-dimethylpyridine (Ivin), sodium and potassium salts of 6-methyl-2-mercapto-4hydroxypyrimidine (Methyur and Kamethur), 6-methyl-2-propylsulfanyl-pyrimidin-4-ol, and sodium 4hydroxypyrimidine-2-thiolate, respectively. A comparative analysis of the growth-regulating activity of auxins showed that the morphometric parameters obtained on 2-week-old barley plants treated with IAA and NAA, exceeded similar parameters of control plants, as follows: plant shoot length by-7.51-20.57 %, plant root lengthby 15.45-32.81 %, plant biomassby 39.59-49.01 %, respectively. It was shown that pyrimidine and pyridine derivatives at a concentration of 10-7 M exhibit cytokinin-like activity in increasing the content of photosynthetic pigments in the leaves of barley plants. The content of chlorophyll a increased by 6.46-68.33%, chlorophyll b increased by 6.2-64.11%, chlorophyll a+b increased by 6.37-66.81%, carotenoids increased by 2.65-29.81%, respectively, compared with similar parameters of control barley plants treated with distilled water. The practical use of the synthetic compounds, pyrimidine and pyridine derivatives: Methyur, Kamethur, Ivin, and other selected most active synthetic compounds for improving the development of shoots and roots, increasing the biomass of spring barley (Hordeum vulgare L.) variety Avatar in the vegetative phase, and increasing the content of photosynthetic pigments in plant leaves is proposed.
Our chapter is devoted to the biological screening of new effective substitutes of plant hormones... more Our chapter is devoted to the biological screening of new effective substitutes of plant hormones auxins and cytokinins among synthetic low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidines, pyrazolotriazinones, oxazoles, oxazolopyrimidines and isoflavonoids. The auxin-like and cytokinin-like activity of chemical low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole was studied. A specific bioassay on auxin-like activity showed a high stimulating effect of the chemical heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole and isoflavones used at the concentration 10–8 M on the formation of adventitious roots on the 14th-day-old leaf petioles isolated from seedlings of haricot bean (Phaseolus vulgaris L.) cultivar Belozernaya, which was similar or higher of the effect of plant hormones auxins IAA and NAA used at the same concentration 10−8 M. A specific bioassay on cytokinin-like activity showed a high stimulating effect of the chemical heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole used at the concentrations 10–8 M and 10–9 M on the growth of biomass of 16th-day-old cotyledons isolated from seeds of muscat pumpkin (Cucurbita moschata Duch. et Poir.) cultivar Gilea, which was similar or higher of the effect of plant hormone cytokinin Kinetin used at the same concentrations 10–8 M and 10–9 M. The results obtained confirmed the inducing auxin-like and cytokinin-like effect of chemical low molecular weight heterocyclic compounds, derivatives of pyridine, pyrimidine, pyrazole, isoflavones, oxazolopyrimidine and oxazole on plant cell elongation, division, and differentiation that are the basic processes of plant growth. The practical application of derivatives of pyrimidine, pyrazole, isoflavones, pyridine, oxazolopyrimidine and oxazole as new plant growth regulators was proposed. Keywords Plant hormones IAA NAA Kinetin Pyridine Pyrimidine Pyrazole Isoflavones Oxazolopyrimidine Oxazole Phaseolus vulgaris Cucurbita moschataDuch. et Poir
Wheat is one of the economically important cereals that are widely used in the agricultural sect... more Wheat is one of the economically important cereals that are widely used in the agricultural sector of many countries. A very important issue is the development of new effective growth regulators to improve the growth and development of this crop in order to increase its productivity. In our investigations the regulating activity of the synthetic low molecular weight heterocyclic compounds, pyrimidine derivatives on growth and development of wheat (Triticum aestivum L.) variety Tyra was studied. It was found that the use of pyrimidine derivatives in a concentration of 10-7M in water solution revealed a positive effect on the growth and development of both shoot and root systems of wheat plants during the vegetation period. Comparative analysis of the growth regulating activity of synthetic compounds showed that the activity of pyrimidine derivatives was differentiated and depended on substituents in their chemical structure. The application of pyrimidine derivatives as new effective...
The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of... more The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of new effective and ecologically safe plant growth regulating substances on the base of synthetic low molecular weight five and six-membered heterocyclic compounds derivatives of pyridine, pyrimidine, pyrazole, oxazole, and isoflavones as an effective substitutes of phytohormones and traditional plant growth regulators to develop agricultural crops with genetically improved commercially important traits such as accelerated growth and development, increased plant productivity and improved quality of production, and enhanced plant tolerance to abiotic stress factors such as drought, salinity, heat, chilling, osmotic stress, soil pollution by anthropogenic factors, and to biotic stress factors such as pathogens and pests [1]. Maize (Zea mays L.) is ancient cereal crop traditionally used as source for dietary food and pharmaceutical industry due to high content of nutrients such as lipids, proteins, carbohydrates, vitamins, dietary fiber and biological active compounds in the seed and seed oil, and as raw material for the biofuel industry and animal feed [2]. Our work was aimed to study of plant growth regulating activity of new synthetic low molecular weight heterocyclic compounds phosphorylated derivatives of oxazole and pyrimidine with perspective their application as a new effective substitutes of phytohormones for improving of cultivation of maize (Zea mays L.) hybrid Palmyra FAO 190. The conducted in the laboratory conditions researches showed high growth stimulating auxin-like effect of tested chemical compounds on germination of seeds, growth and development of maize seedlings during the vegetation. The comparative statistical analysis showed that the biometric indexes of 14 th-day-old seedlings of maize grown on the water solution of heterocyclic compounds used at concentration 10-9 M were higher than the biometric indexes of 21 st-day-old seedlings of maize grown either on the distilled water (control) or on the water solution of auxins IAA and NAA used at the same concentration 10-9 M in average: at the 23 %-by total length of seedlings, 5-37 %-by total length of roots, and at the 7-87 %-by total number of roots. The positive effect of some from tested chemical heterocyclic compounds on increase of synthesis of photosynthetic pigments in the leaves of 14 th-day-old seedlings of maize was also found: the content of chlorophyll a was increased in average at the 5-10 %; the content of chlorophyll b was increased in average at the 10-74 %; the content of chlorophylls a+b was increased in average at the 5-11 %. The practical application in the agricultural biotechnology of synthetic low molecular weight heterocyclic compounds phosphorylated derivatives of oxazole and pyrimidine as new effective substitutes of phytohormones for improving of growth and development of maize (Zea mays L.) hybrid Palmyra FAO 190 was proposed. 1. V. Tsygankova, Y. Andrusevich, O. Shtompel, O. Myroljubov, A. Hurenko, R. Solomyanny, G. Mrug, M. Frasinyuk, O. Shablykin, V. Brovarets. Study of Auxin, Cytokinin and Gibberellin-like Activity of Heterocyclic Compounds Derivatives of Pyrimidine, Pyridine, Pyrazole and Isoflavones. European Journal of Biotechnology and Bioscience, 2016, 4(12): 29-44. 2. Shah T.R., Prasad K., Kumar P. Maize-A potential source of human nutrition and health: A review. Cogent Food & Agriculture. 2016, 2: 1166995.
The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of... more The recent innovation strategy used in the modern agricultural biotechnology is an elaboration of new effective and ecologically safe plant growth regulating substances on the base of synthetic low molecular weight five and six-membered heterocyclic compounds derivatives of pyridine, pyrimidine, pyrazole, oxazole, and isoflavones as an effective substitutes of phytohormones and traditional plant growth regulators to develop agricultural crops with genetically improved commercially important traits such as accelerated growth and development, increased plant productivity and improved quality of production, and enhanced plant tolerance to abiotic stress factors such as drought, salinity, heat, chilling, osmotic stress, soil pollution by anthropogenic factors, and to biotic stress factors such as pathogens and pests [1-3]. Tomato is a commercially important food crop in the world. Tomato is a widely used crop, moreover, it is a source of carotenoids and flavonoids [4]. The protective properties of carotenoids are mainly due to their high antioxidant activity, as well as the antioxidant activity attributed to lycopene, which is an acyclic isomer of beta-carotene and is abundantly contained in red tomatoes [5]. Unlike beta-carotene, lycopene can not be metabolized to vitamin A in the human body, but it has a dual antioxidant potential of beta-carotene. The using of tomato and tomato products that contain lycopene reduces the risk of developing chronic diseases, such as cancer, cardiovascular and some age-related diseases [6]. This work is aimed to study of stimulating effect of new synthetic low molecular weight compounds derivatives of pyrimidine, on the growth and development of tomato (Solanum lycopersicum L.) plants under laboratory conditions. Key words: tomato (Solanum lycopersicum L.), auxins IAA and NAA, cytokinin Kinetin, synthetic low molecular weight heterocyclic compounds, pyrimidine, plant growth regulating activity, agricultural biotechnology.
Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used i... more Linum usitatissimum L. (flax or linseed) is one of the oldest economically important crops used in biotechnology as a source for bioactive compounds. Flax fiber and seed have industrial significance as a source of cellulosic fiber for textile and paper industry and seed oil for pharmaceutical, cosmetic and food industry. The elaboration of new effective techniques for in vitro regeneration of Linum usitatissimum L. is very actual problem today. The influence of low molecular weight five and six membered heterocyclic compounds on organogenesis of Linum usitatissimum L. cultivar heavenly in vitro has been studied. It was found that heterocyclic compounds derivatives pyridine, pyrimidine, pyrazole and isoflavones revealed high stimulating effect on direct shoot organogenesis of Linum usitatissimum L. in vitro. The maximal shoot regeneration frequency was obtained on the MS media containing heterocyclic compounds at the concentration 10-8 M/l as compared to lower regeneration frequency obtained on the control MS medium containing 1 mg/l BAP and 0.05 mg/l NAA. The maximal index of explants with regeneration (in %) that exceeded almost twice the same index in control MS medium was obtained on the MS medium containing heterocyclic compound derivative of isoflavones. At the same time, the index of explants with regeneration (in %) that exceeded one and a half times the same index in control MS medium and the maximal index of shoots elongated more than 1 cm (in %) that exceeded almost twice the same index in control MS medium were obtained on the MS media containing heterocyclic compounds derivatives of pyridine, pyrimidine and pyrazole. The current study confirms perspective of using of low molecular weight five and six-membered heterocyclic compounds at the concentration 10-8 М/l of the MS medium as new effective substitutes of traditional growth regulators auxin NAA and cytokinin BAP for regeneration of Linum usitatissimum L. cultivar heavenly in vitro. Keywords: screening of new plant growth regulators, nitrogen-containing heterocyclic compounds, pyridine, pyrimidine, pyrazolotriazinone and isoflavones, Linum usitatissimum L., direct shoot organogenesis in vitro
Application of Oxazole and Oxazolopyrimidine as New Effective Regulators of Oilseed Rape Growth V... more Application of Oxazole and Oxazolopyrimidine as New Effective Regulators of Oilseed Rape Growth Victoria Tsygankova*, Yaroslav Andrusevich, Victor Kopich, Olexandra Shtompel, Yevheniia Veligina, Stepan Pilyo, Maryna Kachaeva, Andrii Kornienko, Volodymyr Brovarets Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 1, Murmanskaya str., Kyiv, Ukraine.
Tomato (Solanum lycopersicum L.) is a major agricultural crop cultivated over the world. The elab... more Tomato (Solanum lycopersicum L.) is a major agricultural crop cultivated over the world. The elaboration of new effective plant growth regulators for improving growth of tomato is an important task for plant biotechnology. In the present work the comparative effect of plant hormones auxins IAA and NAA, cytokinin Kinetin, and new synthetic compounds pyrimidine derivatives on acceleration of vegetative growth of tomato was studied. The obtained biometric indices of 24 th-day-old tomato seedlings grown on the water solution of pyrimidine derivatives used at the concentration 10-8 M were similar or higher of the biometric indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin used at the same concentration 10-8 M in average: at the 29-255 %, 22-235 %, 10-156 %, and 13-95 %-by total number of roots, and at the 23-255 %, 21-115 %, 19-138 %, and 9-61 %-by total length of roots, respectively. The content of photosynthetic pigments in tomato seedlings grown on the water solution of pyrimidine derivatives was increased in average: chlorophyll a-at the 17-116 %, 17-122 %, 19-22 %, and 19-52 %; chlorophyll bat the 19-155 %, 21-161 %, 12-45 %, and 11-81 %; chlorophylls a+b-at the 16-126 %, 13-132 %, 23-28 %, and 14-60 %; carotenoids-at the 15-86 %, 19-107 %, 20-31%, and 16-50 %, as compared with similar indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin, respectively. The content of total soluble proteins in tomato seedlings grown on the water solution of pyrimidine derivatives was increased in average: at the 40-241 %, 9-145 %, 15-122 %, and 17-123 %, as compared with similar indices of tomato seedlings grown on the distilled water (control), or on the water solution of IAA, NAA and Kinetin, respectively. The relationship between chemical structure and plant growth regulatory activity of pyrimidine derivatives was set. The highest growth regulatory activity revealed the compounds, derivatives of dihydroimidazo[1,2-c]pyrimidine. The obtained results confirmed the possibility of application of pyrimidine derivatives as new effective regulators of vegetative growth of tomato.
European Journal of Biotechnology and Bioscience, 2016
Impact of microbial biostimulants on the increase (up to 75-87%) of resistance of wheat plants of... more Impact of microbial biostimulants on the increase (up to 75-87%) of resistance of wheat plants of two varieties Yatran 60 and Zimoyarka to the cereal cyst nematode Heterodera avenae was found. The increase of difference in the index of Dot-blot hybridization (up to 37-65%) between cytoplasmic mRNA and si/miRNA isolated from control and experimental (affected by nematode and treated with biostimulants) plants was observed. In the wheat embryo cell-free system the increase of silencing activity (up to 48-78%) of si/miRNA isolated from experimental as compared to control wheat plants was revealed. These data indicate that microbial biostimulants cause reprogramming wheat genome by inducing synthesis si/miRNA with immuneprotective properties against nematode; as a result the wheat resistance to nematode invasion is increased. To obtain new lines of wheat cells resistant to nematode H. avenae the microbial biostimulants were used for preparing of MS media for cultivation of isolated whea...
This book covers all areas of biotechnology, genetics and other related fields. The contributions... more This book covers all areas of biotechnology, genetics and other related fields. The contributions by the authors include biosurfactants; antimicrobial activity; bio-preservation; shelf life; plasmid; algal plasmid; plasmid like DNA; fungal contamination; mycotoxins; Detarium microcarpum; Brachystegia eurycoma; Huanglongbing disease; Candidatus liberibacter asiaticus; citrus; Jatropha curcas; euphorbiaceae; essential oil; palmitic acid; linoleic acid; alloisolongifolene; β-farnesene; moisture barrier; shelf-life of horticultural produce; SGOT; SGPT; alloxan monohydrate; aspirin; membrane stabilization; cellulase activity; actinomycetes; fermentation; carboxymethyl cellulose; xylanolytic activity; Trametes sp.; Annona senegalensis; Oulotricha subspecies; thermophiles; bacterial isolates; antibiotic resistance; pathogenicity; urinary tract infections; Dental Pulp Stem Cells (DPSC); Bone Morphogenic Protein (BMP-2) etc. This book contains various materials suitable for students, researchers and academicians in the field of biotechnology and genetics. Key words: Biotechnology, Genetics
Book Publisher International. SCIENCEDOMAIN international Ltd. ISBN 978-81-19491-04-9 (Print), ISBN 978-81-19491-05-6 (eBook), 2023
This book covers key areas of chemistry and biochemistry. The contributions by the authors includ... more This book covers key areas of chemistry and biochemistry. The contributions by the authors include cytogenetic methods, plant test-system, animal test-system in vivo, human lymphocytes in vitro, Pr3+-doped CdWO4 nanoparticles, fiber morphology, phenylbenzopyrone, flavonoids, anti-oxidant, anti-inflammmatory, immunomodulatory, antiviral properties, antimutagenic and anticarcinogenic properties, new schiff base complexes, pulp blending, chipping, beating, physical strength, metal complexes, anti-cancer activity, cell viability, photocatalytic, kinetic study, current photocatalytic system, metal tungstates, Scheelite, wolframite, stoichiometric amount, charge transfer molecules, molecular docking, MEP mapping and mulliken charge distribution, thermogravimetric analysis, Brunauer-Emmett-Teller (BET) analysis, carboxyl group, inflammatory proteins, acrylate-based high internal Phase emulsions, differential scanning calorimetry, chemical potential and electrophilic index. This book contains various materials suitable for students, researchers and academicians in the field of chemistry and biochemistry.
Book Publisher International. SCIENCEDOMAIN international Ltd. India.United Kingdom. ISBN 978-81-19315-43-7 (Print) ISBN 978-81-19315-40-6 (eBook), 2023
This book covers key areas of Microbiology and Biotechnology. The contributions by the authors in... more This book covers key areas of Microbiology and Biotechnology. The contributions by the authors include biosciences, biotechnology, bioethics, catalase, purification, enzyme characterization, bacteriocins, serum cholesterol, fermentation, lactobacilli, crop production, organic, mineral compounds, endophytic fungi. This book contains various materials suitable for students, researchers and academicians in the field of Microbiology and Biotechnology.
Book Publisher International. SCIENCEDOMAIN international Ltd.ISBN 978-81-19217-26-7 (Print), ISBN 978-81-19217-06-9 (eBook), 2023
This book covers key areas of chemistry and biochemistry. The contributions by the authors includ... more This book covers key areas of chemistry and biochemistry. The contributions by the authors include heavy metals, effluents, anthropogenic activities, environmental pollution, polyaromatic hydrocarbon, adenine-guanine transition, SMD simulations, solution reaction mechanisms, genetic mutation, agricultural wastes, aquatic system, mutagenic effect, electro dialysis, reverse osmosis, electrochemical treatments, kidney dysfunction, hepatic damage, hypertension, phosphate solubilizing microbes, oxidation pathway, bioproducts, biorefinery, lignocellulosic biomass, technical lignin, flavonoids, lipid peroxidation, hydrolytic stability, thermal stability, adsorption, copper ions, agricultural waste, acetylcholine receptors, hydrolases, photo switches, diazo carbonyls, thin layer chromatography, anorexia, eating disorder, fatty acid molecules, redundant, isotherms, herbicide, and biowaste. This book contains various materials suitable for students, researchers and academicians in the field of chemistry and biochemistry.
Research Advances in Microbiology and Biotechnology Vol. 3 / Ed. Dr. Tsygankova Victoria Anatolyivna. Book Publisher International. SCIENCEDOMAIN international Ltd. 2023. 166 p. ISBN 978-81-19102-24-2 (Print) ISBN 978-81-19102-26-6 (eBook)., 2023
This book covers key areas of Microbiology and Biotechnology. The contributions by the authors in... more This book covers key areas of Microbiology and Biotechnology. The contributions by the authors include biosafety, risk assessment, antifungal activity, biocontrol agent, polymerase chain reaction assays, DNA sequencing, molecular characteristics, bacteriophages, antimicrobial resistance, lytic phages, lysogenic phages, biofilm, amide derivatives, antimicrobial activity, disk diffusion method, molecular identification, low postharvest physiological deterioration, COVID-19, thromboembolic events, arterial thrombotic events, venous thrombotic events, bleedings, hemostasis, and anticoagulant therapy. This book contains various materials suitable for students, researchers and academicians in the field of Microbiology and Biotechnology.
МАТЕРІАЛИ ДОПОВІДЕЙ V МІЖНАРОДНОЇ НАУКОВО-ПРАКТИЧНОЇ КОНФЕРЕНЦІЇ «Розвиток аграрної галузі та впровадження наукових розробок у виробництво». (19-21 жовтня 2022 р.) Миколаїв., 2022
Метою цієї роботи є вивчення впливу мікродобрив Росток Екстра виробництва ТОВ «Український Аграрн... more Метою цієї роботи є вивчення впливу мікродобрив Росток Екстра виробництва ТОВ «Український Аграрний Ресурс» і Radix Tim forte plus виробництва компанії Forcrop окремо та у комплексному застосуванні із синтетичними регуляторами росту рослин Івіном, Метіуром і Каметуром на покращення ростових показників важливої зернової культури – озимої пшениці (Triticum aestivum L.) сорту Шестопалівка протягом періоду вегетації у лабораторних умовах. З цією метою насіння пшениці обробляли розчинами мікродобрив: Росток Екстра (концентрація розчину 100 мл на 1л води), або Radix Tim forte plus (концентрація розчину 50 мл на 1л води), або кожним з регуляторів росту рослин Івіном, Метіуром або Каметуром (концентрація 10-7М/л розчину) окремо, або у комплексі з кожним із зазначених мікродобрив у вказаних вище концентраціях. Оброблене насіння вирощували у лабораторних умовах протягом 30-ти діб. Порівняльний аналіз ростових показників рослин пшениці (середньої довжини пагонів, середньої довжини найдовшого кореня, середньої довжини коренів, середньої біомаси 10 рослин) показав позитивний вплив мікродобрив Росток Екстра і Radix Tim forte plus на ці показники рослин при їх застосуванні окремо або у комплексі із синтетичними регуляторами росту Івіном, Метіуром і Каметуром. Запропоновано практичне використання мікродобрив Росток Екстра і Radix Tim forte plus у комплексі із синтетичними регуляторами росту Івіном, Метіуром і Каметуром для поліпшення росту та розвитку зернової культури – озимої пшениці (Triticum aestivum L.) сорту Шестопалівка протягом періоду вегетації рослин.
Міжнародна науково-практична онлайн конференція, присвячена 60-річчю спеціальності «Захист і карантин рослин» «Інноваційні технології в захисті рослин за умов глобалізації». МІНІСТЕРСТВО ОСВІТИ І НАУКИ УКРАЇНИ НАІОНАЛЬНИЙ УНІВЕРСИТЕТБІОРЕСУРСІВ І ПРИРОДОКОРИСТУВАННЯ УКРАЇНИ, 2022
Досліджено протекторний ефект біостимуляторів Біосил та Радостим на стійкість пшениці озимої (Tri... more Досліджено протекторний ефект біостимуляторів Біосил та Радостим на стійкість пшениці озимої (Triticum aestivum L.) до водного дефіциту відповідно фізіологічних показників рослин та молекулярно-генетичних маркерів резистентності рослин - малих регуляторних РНК (si/miРНК).
This book covers key areas of Microbiology and Biotechnology. The contributions by the authors in... more This book covers key areas of Microbiology and Biotechnology. The contributions by the authors include syphilis, laboratory tests, genomic expression analysis, single cell, rotavirus, genotypes, vaginal microflora, reproductive diseases, opportunistic pathogens, bacterial population, mushroom, basidiocarp, basidiomycotina, mycelium, basidiospores, vegetable oil spill, bioremediation, bacteria artificial chromosome, and cassava mosaic disease. This book contains various materials suitable for students, researchers and academicians in the field of Microbiology and Biotechnology.
This book covers key areas of Microbiology and Biotechnology. The contributions by the authors in... more This book covers key areas of Microbiology and Biotechnology. The contributions by the authors include syphilis, laboratory tests, genomic expression analysis, single cell, rotavirus, genotypes, vaginal microflora, reproductive diseases, opportunistic pathogens, bacterial population, mushroom, basidiocarp, basidiomycotina, mycelium, basidiospores, vegetable oil spill, bioremediation, bacteria artificial chromosome, and cassava mosaic disease. This book contains various materials suitable for students, researchers and academicians in the field of Microbiology and Biotechnology.
Book: Synthesis and bioactivity of functionalized nitrogen-containing heterocycles / Eds. A.I. Vovk. Kyiv: Interservice, 2021
The review is devoted to the solution of an actual problem: the development of new effective and... more The review is devoted to the solution of an actual problem: the development of new effective and ecologically safe plant growth regulators based on low molecular weight heterocyclic compounds of the azoles, azines and their condensed derivatives. Data from the world literature devoted to the screening of auxin- and cytokinin-related compounds of natural or synthetic origin using modern SAR (Structure-Activity Relationship) analysis of compounds are presented. The data of experimental studies of the regulatory activity of low-molecular heterocyclic compounds of the classes of azoles, azines and their condensed derivatives on the growth and development of various types of plants during their ontogenesis in laboratory and field conditions, as well as on the processes of organogenesis and regeneration of plants in the conditions of isolated cells and plant tissues in vitro are presented . The issue of the possibility of using the most promising chemical compounds selected in the process of biological screening, which show high regulatory activity of plant growth and development, in the practice of agriculture and biotechnology is discussed. Key words: plant growth regulators, auxins, cytokinins, azoles, azines and their condensed derivatives.
Proceedings of the 3rd International Electronic Conference on Agronomy, 15–30 October 2023, MDPI: Basel, Switzerland, 2023
THE USE OF NEW PLANT GROWTH REGULATORS METHYUR, KAMETHUR AND IVIN TO INCREASE THE PRODUCTIVITY OF... more THE USE OF NEW PLANT GROWTH REGULATORS METHYUR, KAMETHUR AND IVIN TO INCREASE THE PRODUCTIVITY OF SORGHUM
Victoria Anatolyivna Tsygankova * 1 , Iryna Valeriivna Voloshchuk 1 , Stepan Grihorovych Pilyo 2 , Svitlana Viktorivna Klyuchko 1 , Volodymyr Serhiyovych Brovarets 2
1 Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry (IBOPC) of the National Academy of the Sciences (NAS) of Ukraine, Kyiv, Ukraine, East Europe
2 Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 1, Academician Kukhar str., 02094, Kyiv-94, Ukraine
Academic Editor: Gianni Bellocchi
Published: 26 October 2023 by MDPI in The 3rd International Electronic Conference on Agronomy session Poster session https://doi.org/10.3390/IECAG2023-15222 (registering DOI)
Abstract:
Sorghum is one of the most important food, fodder and technical crops grown in the world. Global climate change and environmental pollution with toxic industrial and agricultural waste are the most unfavorable environmental factors affecting the growth and development of sorghum, which leads to a decrease in product quality. The main purpose of this work is to study the effect of new plant growth regulators Methyur (derivative of sodium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine), Kamethur (derivative of potassium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine) and Ivin (N-oxide-2,6-dimethylpyridine) on the productivity of field-grown sorghum. Field experiments were carried out on grain sorghum (Sorghum bicolor L.) cv. Yarona, and sweet sorghum (Sorghum saccharatum L.) cv. Favorite. Statistical analysis showed that the productivity parameters of 4 month-old sorghum plants obtained from seeds treated before sowing in the soil with water solution of Methyur, Kamethur or Ivin at a concentration of 10-7M, increased as follows: the average panicle length (in cm) increased by 7% - 20% - for grain sorghum cv. Yarona and by 25% - 37% - for sweet sorghum cv. Favorite, accordingly, and the average fresh weight of grain (in gram) increased by 13% – 26% - for grain sorghum cv. Yarona, and by 24% - 38% - for sweet sorghum cv. Favorite, compared to control.
Global Meet on Food Science and Technology. March 23, 2023. Webinar GMFST 2023, LAS VEGAS, USA., 2023
Global climate change and environmental pollution negatively affect growth and reduce the yield o... more Global climate change and environmental pollution negatively affect growth and reduce the yield of the important cereal crop wheat (Triticum aestivum L.). Our research is aimed at creating new effective and environmentally friendly wheat growth regulators based on pyridine and pyrimidine derivatives synthesized in V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine. In our experiments, we studied the plant growth regulatory activity of both known plant growth regulators based on pyridine and pyrimidine derivatives: Ivin (N-oxide-2,6-dimethylpyridine), Methyur (sodium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine) and Kamethur (potassium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine), as well as new chemical compounds, pyrimidine derivatives: №1 - 2-ethylsulfanyl-6-methylpyrimidin-4-ol; №2 – 6-methyl-2-propylsulfanyl-pyrimidin-4-ol; №3 – 2-benzylsulfanyl-6-methylpyrimidin-4-ol; №4 – 2-isopropyl-6-methyl-pyrimidin-4-ol; №5 – sodium 4-hydroxypyrimidine--2-thiolate; №6 – 2-methylsulfanylpyrimidin-4-ol; №7 – 2-benzylsulfanylpyrimidin-4-ol. The plant growth regulatory activity of pyridine and pyrimidine derivatives was compared with the activity of plant hormones - auxins IAA (1H-indol-3-yl)acetic acid) and NAA (1-naphthylacetic acid), which were used in a concentration of 10-7М. Experiments have shown that pyridine and pyrimidine derivatives, which were used in a similar concentration of 10-7M, exhibit plant growth regulatory activity similar to auxins IAA and NAA, improving the formation and growth of roots and shoots of spring wheat (Triticum aestivum L.) variety Svitlana within two weeks. The highest plant growth regulatory activity was shown by known chemical compounds pyridine and pyrimidine derivatives: Ivin, Methyur and Kamethur, as well as new chemical compounds, pyrimidine derivatives № 1, 2, 4 and 7. Growth parameters of wheat plants grown on solutions of these compounds exceeded the parameters of control plants grown on distilled water, and equaled or exceeded the parameters of plants grown on solutions of auxins IAA and NAA. The average shoot length (cm) increased: by 26.19 % - under the influence of IAA, by 20.71 % - under the influence of NAA, by 47.43 % - under the influence of Methyur, by 43.67 % - under the influence of Kamethur, by 41.43 % - under the influence Ivin, by 18.1 – 28.89 % - under the influence of compounds № 1, 2, 4 and 7, respectively, compared to similar parameters of control wheat plants grown on distilled water. The average root length (cm) increased: by 27.72 % - under the influence of IAA, by 23.17 % - under the influence of NAA, by 33.37 % - under the influence of Methyur, by 36.49 % - under the influence of Kamethur, by 43.80 % - under the influence of Ivin, by 24.31 – 47.33 % - under the influence of compounds № 2 and 4, respectively, compared to similar parameters of control wheat plants grown on distilled water. The use of chemical compounds of pyridine and pyrimidine derivatives: Ivin, Methyur and Kamethur, as well as compounds № 1, 2, 4 and 7 acting in nanomolar concentrations, non-toxic to humans and animals, as new wheat growth regulators is proposed. Keywords: wheat; auxins; pyridine; pyrimidine.
The international conference "Innovative technologies in plant protection under the conditions of globalization" is dedicated to the 60th anniversary of the "Plant protection and quarantine" specialty. The National University of Life and Environmental Sciences of Ukrain, Kyiv, 2022
The effectiveness of the use of biostimulants Regoplant and Stimpo to increase the resistance of ... more The effectiveness of the use of biostimulants Regoplant and Stimpo to increase the resistance of wheat plants to pathogenic micromycetes Fusarium graminearum in accordance with plant physiological parameters and molecular genetic markers of plant resistance - small regulatory RNAs (si/miRNAs) is shown.
The international conference "Innovative technologies in plant protection under the conditions of globalization" is dedicated to the 60th anniversary of the "Plant protection and quarantine" specialty. The National University of Life and Environmental Sciences of Ukrain, Kyiv,, 2022
The protective effect of biostimulants Biosil and Radostim on the resistance of winter wheat (Tri... more The protective effect of biostimulants Biosil and Radostim on the resistance of winter wheat (Triticum aestivum L.) to water deficit in accordance with plant physiological parameters and molecular genetic markers of plant resistance - small regulatory RNAs (si/miRNAs) was shown.
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Papers by Victoria Tsygankova
Keywords: oilseed flax (Linum usitatissimum L.), Ivin, Methyur, Kamethur, Rostok Extra, Radix Tim forte+.
as well as new synthetic compounds, pyrimidine derivatives, on growth and photosynthesis of microgreens of pea (Pisum sativum
L.) variety Slobozhansky whiskered was studied. The growth-regulating effect of synthetic plant growth regulators
Methyur, Kamethur, Ivin, as well as new pyrimidine derivatives, was compared with the growth-regulating effect of phytohormones
auxins IAA and NAA. The results obtained showed that synthetic plant growth regulators Methyur, Kamethur
and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, have both auxin-like and
cytokinin-like effect on enhancing the growth of shoots and roots of three-week-old microgreens of pea (Pisum sativum L.)
variety Slobozhansky whiskered. It was also found that the synthetic plant growth regulators Methyur, Kamethur and Ivin,
as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, exhibit a cytokinin-like effect on the
increasing the content of photosynthetic pigments (chlorophylls a, b, a+b and carotenoids) in the leaves of three-week-old
microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. The use of synthetic plant growth regulators Methyur,
Kamethur and Ivin, as well as new synthetic pyrimidine derivatives at a concentration of 10-7M to improve the growth of
shoots and the roots of pea microgreens, as well as to increase the content of chlorophylls a, b, a+b and carotenoids in pea
microgreens is proposed.
Keywords: Pea microgreens; Shoots and Roots, Chlorophylls a, b, a+b and Carotenoids; IAA and NAA; Methyur; Kamethur; Ivin; Pyrimidine and Pyridine Derivatives.
Keywords: oilseed flax (Linum usitatissimum L.), Ivin, Methyur, Kamethur, Rostok Extra, Radix Tim forte+.
as well as new synthetic compounds, pyrimidine derivatives, on growth and photosynthesis of microgreens of pea (Pisum sativum
L.) variety Slobozhansky whiskered was studied. The growth-regulating effect of synthetic plant growth regulators
Methyur, Kamethur, Ivin, as well as new pyrimidine derivatives, was compared with the growth-regulating effect of phytohormones
auxins IAA and NAA. The results obtained showed that synthetic plant growth regulators Methyur, Kamethur
and Ivin, as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, have both auxin-like and
cytokinin-like effect on enhancing the growth of shoots and roots of three-week-old microgreens of pea (Pisum sativum L.)
variety Slobozhansky whiskered. It was also found that the synthetic plant growth regulators Methyur, Kamethur and Ivin,
as well as new synthetic compounds, pyrimidine derivatives at a concentration of 10-7M, exhibit a cytokinin-like effect on the
increasing the content of photosynthetic pigments (chlorophylls a, b, a+b and carotenoids) in the leaves of three-week-old
microgreens of pea (Pisum sativum L.) variety Slobozhansky whiskered. The use of synthetic plant growth regulators Methyur,
Kamethur and Ivin, as well as new synthetic pyrimidine derivatives at a concentration of 10-7M to improve the growth of
shoots and the roots of pea microgreens, as well as to increase the content of chlorophylls a, b, a+b and carotenoids in pea
microgreens is proposed.
Keywords: Pea microgreens; Shoots and Roots, Chlorophylls a, b, a+b and Carotenoids; IAA and NAA; Methyur; Kamethur; Ivin; Pyrimidine and Pyridine Derivatives.
Victoria Anatolyivna Tsygankova * 1 , Iryna Valeriivna Voloshchuk 1 , Stepan Grihorovych Pilyo 2 , Svitlana Viktorivna Klyuchko 1 , Volodymyr Serhiyovych Brovarets 2
1 Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry (IBOPC) of the National Academy of the Sciences (NAS) of Ukraine, Kyiv, Ukraine, East Europe
2 Department for Chemistry of Bioactive Nitrogen-Containing Heterocyclic Compounds, V.P. Kukhar Institute of Bioorganic Chemistry and Petrochemistry, National Academy of Sciences of Ukraine, 1, Academician Kukhar str., 02094, Kyiv-94, Ukraine
Academic Editor: Gianni Bellocchi
Published: 26 October 2023 by MDPI in The 3rd International Electronic Conference on Agronomy session Poster session https://doi.org/10.3390/IECAG2023-15222 (registering DOI)
Abstract:
Sorghum is one of the most important food, fodder and technical crops grown in the world. Global climate change and environmental pollution with toxic industrial and agricultural waste are the most unfavorable environmental factors affecting the growth and development of sorghum, which leads to a decrease in product quality. The main purpose of this work is to study the effect of new plant growth regulators Methyur (derivative of sodium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine), Kamethur (derivative of potassium salt of 6-methyl-2-mercapto-4-hydroxypyrimidine) and Ivin (N-oxide-2,6-dimethylpyridine) on the productivity of field-grown sorghum. Field experiments were carried out on grain sorghum (Sorghum bicolor L.) cv. Yarona, and sweet sorghum (Sorghum saccharatum L.) cv. Favorite. Statistical analysis showed that the productivity parameters of 4 month-old sorghum plants obtained from seeds treated before sowing in the soil with water solution of Methyur, Kamethur or Ivin at a concentration of 10-7M, increased as follows: the average panicle length (in cm) increased by 7% - 20% - for grain sorghum cv. Yarona and by 25% - 37% - for sweet sorghum cv. Favorite, accordingly, and the average fresh weight of grain (in gram) increased by 13% – 26% - for grain sorghum cv. Yarona, and by 24% - 38% - for sweet sorghum cv. Favorite, compared to control.
Keywords: wheat; auxins; pyridine; pyrimidine.