Chilli (Capsicum annuum L.) is one of the most important vegetable as well as spice crops grown w... more Chilli (Capsicum annuum L.) is one of the most important vegetable as well as spice crops grown worldwide for its wide usage. Production of chilli is profoundly affected by anthracnose disease which is one of the most serious and destructive fungal diseases of chilli, especially in tropical and subtropical regions causing around 60–80% yield loss under severe infection. In order to understand the pattern of gene expression and development of molecular markers in chilli for management of anthracnose disease, transcriptome analysis of a resistant (IIVRC-452) and susceptible (Pusa Jwala) genotypes was carried out. Transcriptome data yielded a total of 53,921,012 and 50,079,890 reads with HQ bases 5.18 GB and 4.78 GB of FASTAq sequences and were used for de novo assembly of transcriptome for IIVRC-452 and Pusa Jwala, respectively. More than 50 thousand unigenes were identified in each genotype and around 40,000 of them could be annotated with 30% cut off identity. Digital gene expression analysis revealed a total of 3124 transcripts differentially expressed in resistant and susceptible lines. A total of 871 differential transcripts were annotated and 814 differential genes were present in both resistant and susceptible lines. Furthermore, five key candidate genes (CaLOX, CaLAG-1, CaPG, CaCYP76A2, and CaSAP-13) in pathogen-responsive pathway were identified by quantitative real-time PCR (qRT-PCR). Transcriptome data generated in the present study is a valuable resource for focused investigation on plant–pathogen-interaction, to study the pathways involved in resistance mechanism and to identify markers for use in resistance breeding programmes.
Eggplant has limited shelf-life of only 3 days under ambient storage conditions. The functional q... more Eggplant has limited shelf-life of only 3 days under ambient storage conditions. The functional quality of carnauba wax (CW) is assessed with various additives for shelf-life related attributes in eggplant during ambient storage (20 ± 1 C and RH 52e54%) in both unpackaged and packaged in 35m polypropylene pouches. Minimum decrease (8.56 N-6.92 N and 8.56 N-5.63 N) in firmness was recorded in Poly ethylene glycol (PEG) and 0.5% Sodium alginate (SA) in diluted (1:4) CW emulsion (T 2) while maximum decrease (8.56 N-5.54 N and 8.56 N-3.57 N) in control (T 4) packaged and unpackaged eggplants after 12 days of storage, respectively. Maximum antioxidant activity (67.63 and 51.52 mmol Trolox equivalent antioxidant capacity (TEAC)/100 g FW) was observed in T 2 treatment and minimum antioxidant activity (23.61 and 20.60 mmol TEAC/100 g FW) in control fruits in packaged and unpackaged respectively after 12 days. The minimum decrease (2.64e2.20 and 2.64e2.24) mmol tetraguaiacol (TG)/min/100 g FW) of peroxidase activity was recorded in T 2 treated fruits in packaged and unpackaged eggplant respectively and maximum decrease (2.64e1.37 and 2.64e1.63 mmol TG/min/100 g FW) was obtained in control unpackaged and packaged eggplant respectively after 12 days. Packaged eggplant treated with PEG and SA in CW emulsion remained acceptable upto 12 days.
In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity,... more In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orche-stration of complex molecular networks. In the pre-sent study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, per-formed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with nota-ble differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 a...
In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity,... more In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orche-stration of complex molecular networks. In the pre-sent study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, per-formed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with nota-ble differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 a...
Chilli (Capsicum annuum L.) is one of the most important vegetable as well as spice crops grown w... more Chilli (Capsicum annuum L.) is one of the most important vegetable as well as spice crops grown worldwide for its wide usage. Production of chilli is profoundly affected by anthracnose disease which is one of the most serious and destructive fungal diseases of chilli, especially in tropical and subtropical regions causing around 60–80% yield loss under severe infection. In order to understand the pattern of gene expression and development of molecular markers in chilli for management of anthracnose disease, transcriptome analysis of a resistant (IIVRC-452) and susceptible (Pusa Jwala) genotypes was carried out. Transcriptome data yielded a total of 53,921,012 and 50,079,890 reads with HQ bases 5.18 GB and 4.78 GB of FASTAq sequences and were used for de novo assembly of transcriptome for IIVRC-452 and Pusa Jwala, respectively. More than 50 thousand unigenes were identified in each genotype and around 40,000 of them could be annotated with 30% cut off identity. Digital gene expression analysis revealed a total of 3124 transcripts differentially expressed in resistant and susceptible lines. A total of 871 differential transcripts were annotated and 814 differential genes were present in both resistant and susceptible lines. Furthermore, five key candidate genes (CaLOX, CaLAG-1, CaPG, CaCYP76A2, and CaSAP-13) in pathogen-responsive pathway were identified by quantitative real-time PCR (qRT-PCR). Transcriptome data generated in the present study is a valuable resource for focused investigation on plant–pathogen-interaction, to study the pathways involved in resistance mechanism and to identify markers for use in resistance breeding programmes.
Eggplant has limited shelf-life of only 3 days under ambient storage conditions. The functional q... more Eggplant has limited shelf-life of only 3 days under ambient storage conditions. The functional quality of carnauba wax (CW) is assessed with various additives for shelf-life related attributes in eggplant during ambient storage (20 ± 1 C and RH 52e54%) in both unpackaged and packaged in 35m polypropylene pouches. Minimum decrease (8.56 N-6.92 N and 8.56 N-5.63 N) in firmness was recorded in Poly ethylene glycol (PEG) and 0.5% Sodium alginate (SA) in diluted (1:4) CW emulsion (T 2) while maximum decrease (8.56 N-5.54 N and 8.56 N-3.57 N) in control (T 4) packaged and unpackaged eggplants after 12 days of storage, respectively. Maximum antioxidant activity (67.63 and 51.52 mmol Trolox equivalent antioxidant capacity (TEAC)/100 g FW) was observed in T 2 treatment and minimum antioxidant activity (23.61 and 20.60 mmol TEAC/100 g FW) in control fruits in packaged and unpackaged respectively after 12 days. The minimum decrease (2.64e2.20 and 2.64e2.24) mmol tetraguaiacol (TG)/min/100 g FW) of peroxidase activity was recorded in T 2 treated fruits in packaged and unpackaged eggplant respectively and maximum decrease (2.64e1.37 and 2.64e1.63 mmol TG/min/100 g FW) was obtained in control unpackaged and packaged eggplant respectively after 12 days. Packaged eggplant treated with PEG and SA in CW emulsion remained acceptable upto 12 days.
In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity,... more In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orche-stration of complex molecular networks. In the pre-sent study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, per-formed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with nota-ble differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 a...
In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity,... more In Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orche-stration of complex molecular networks. In the pre-sent study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, per-formed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with nota-ble differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 a...
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Papers by Ashutosh Rai