Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015
The resolving power of spectrophotometric assisted mathematical techniques were demonstrated for ... more The resolving power of spectrophotometric assisted mathematical techniques were demonstrated for the simultaneous determination of perindopril arginin (PER) and amlodipine besylate (AML) in presence of their degradation products. The conventional univariate methods include the absorptivity factor method (AFM) and absorption correction method (ACM), which were able to determine the two drugs, simultaneously, but not in the presence of their degradation products. In both methods, amlodipine was determined directly at 360nm in the concentration range of 8-28μgmL(-1), on the other hand perindopril was determined by AFM at 222.2nm and by ACM at 208nm in the concentration range of 10-70μgmL(-1). Moreover, the applied multivariate calibration methods were able for the determination of perindopril and amlodipine in presence of their degradation products using concentration residuals augmented classical least squares (CRACLS) and partial least squares (PLS). The proposed multivariate methods were applied to 19 synthetic samples in the concentration ranges of 60-100μgmL(-1) perindopril and 20-40μgmL(-1) amlodipine. Commercially available tablet formulations were successfully analysed using the developed methods without interference from other dosage form additives except PLS model, which failed to determine both drugs in their pharmaceutical dosage form.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Jan 5, 2015
Smart and novel spectrophotometric and chemometric methods have been developed and validated for ... more Smart and novel spectrophotometric and chemometric methods have been developed and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and dexamethasone sodium phosphate (DSP) in presence of interfering substances without prior separation. The first method depends upon derivative subtraction coupled with constant multiplication. The second one is ratio difference method at optimum wavelengths which were selected after applying derivative transformation method via multiplying by a decoding spectrum in order to cancel the contribution of non labeled interfering substances. The third method relies on partial least squares with regression model updating. They are so simple that they do not require any preliminary separation steps. Accuracy, precision and linearity ranges of these methods were determined. Moreover, specificity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied for analysis of bo...
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015
This work is concerned with development and validation of three simple, specific, accurate and pr... more This work is concerned with development and validation of three simple, specific, accurate and precise spectrophotometric methods for determination of flumethasone pivalate (FP) and clioquinol (CL) in their binary mixture and ear drops. Method A is a ratio subtraction spectrophotometric one (RSM). Method B is a ratio difference spectrophotometric one (RDSM), while method C is a mean center spectrophotometric one (MCR). The calibration curves are linear over the concentration range of 3-45 μg/mL for FP, and 2-25 μg/mL for CL. The specificity of the developed methods was assessed by analyzing different laboratory prepared mixtures of the FP and CL. The three methods were validated as per ICH guidelines; accuracy, precision and repeatability are found to be within the acceptable limits.
Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitomet... more Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitometric methods were developed for determination of oxybutynin hydrochloride (OX) in presence of its degradation product and additives in its pharmaceutical formulations. Method A is the first derivative of ratio spectra (DD(1)) which allows the determination of OX in presence of its degradate in pure form and tablets by measuring the peaks amplitude at 216 nm. Method B and C are principal component regression (PCR) and partial least-squares (PLS) for determination of OX in presence of its degradate in pure form, tablets and syrup. While, the developed high performance thin layer chromatography HPTLC-densitometric method was based on the separation of OX from its degradation product, methylparaben and propylparaben followed by densitometric measurement at 220 nm which allows the determination of OX in pure form, tablets and syrup. The separation was achieved using HPTLC silica gel F(254) plates and chloroform:methanol:ammonia solution:triethylamine (100:3:0.5:0.2, v/v/v/v) as the developing system. The accuracy, precision and linearity ranges of the developed methods were determined. The results obtained were statistically compared with each other and to that of a reported HPLC method, and there was no significant difference between the proposed methods and the reported method regarding both accuracy and precision.
Journal of Dispersion Science and Technology, 2012
In this paper, three deferent surfactants as corrosion inhibitor were prepared in two steps; In t... more In this paper, three deferent surfactants as corrosion inhibitor were prepared in two steps; In the first step, maleic anhydride was amidated with dodecylamine to produce polyoxy ethylenyl-2-ene-4-dodecanamide butanoic acid. In the second step, the resulting product was further was further esterified with different molecular weights of polyethylene glycol (m. wt. = 200, 400 and 600) namely; MP2, MP4 and MP6. The
Norfloxacin (Nor), (1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7 (1-piperazinyl)-3-quinoline carboxylic a... more Norfloxacin (Nor), (1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7 (1-piperazinyl)-3-quinoline carboxylic acid is a synthetic an-tibacterial drug.1,2) Methods were reported for its determina-tion as titrimetric,3) LC,410) LC-MS,1113) spectrometric,1416) spectrofluorimetric1720) ...
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Jan 8, 2015
Smart spectrophotometric methods have been applied and validated for the simultaneous determinati... more Smart spectrophotometric methods have been applied and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and prednisolone acetate (PA) without preliminary separation. Two novel methods have been developed; the first method depends upon advanced absorbance subtraction (AAS), while the other method relies on advanced amplitude modulation (AAM); in addition to the well established dual wavelength (DW), ratio difference (RD) and constant center coupled with spectrum subtraction (CC-SS) methods. Accuracy, precision and linearity ranges of these methods were determined. Moreover, selectivity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied to the assay of drugs in their pharmaceutical formulations. No interference was observed from common additives and the validity of the methods was tested. The obtained results have been statistically compared to that of official spectrophotometric methods t...
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015
The resolving power of spectrophotometric assisted mathematical techniques were demonstrated for ... more The resolving power of spectrophotometric assisted mathematical techniques were demonstrated for the simultaneous determination of perindopril arginin (PER) and amlodipine besylate (AML) in presence of their degradation products. The conventional univariate methods include the absorptivity factor method (AFM) and absorption correction method (ACM), which were able to determine the two drugs, simultaneously, but not in the presence of their degradation products. In both methods, amlodipine was determined directly at 360nm in the concentration range of 8-28μgmL(-1), on the other hand perindopril was determined by AFM at 222.2nm and by ACM at 208nm in the concentration range of 10-70μgmL(-1). Moreover, the applied multivariate calibration methods were able for the determination of perindopril and amlodipine in presence of their degradation products using concentration residuals augmented classical least squares (CRACLS) and partial least squares (PLS). The proposed multivariate methods were applied to 19 synthetic samples in the concentration ranges of 60-100μgmL(-1) perindopril and 20-40μgmL(-1) amlodipine. Commercially available tablet formulations were successfully analysed using the developed methods without interference from other dosage form additives except PLS model, which failed to determine both drugs in their pharmaceutical dosage form.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Jan 5, 2015
Smart and novel spectrophotometric and chemometric methods have been developed and validated for ... more Smart and novel spectrophotometric and chemometric methods have been developed and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and dexamethasone sodium phosphate (DSP) in presence of interfering substances without prior separation. The first method depends upon derivative subtraction coupled with constant multiplication. The second one is ratio difference method at optimum wavelengths which were selected after applying derivative transformation method via multiplying by a decoding spectrum in order to cancel the contribution of non labeled interfering substances. The third method relies on partial least squares with regression model updating. They are so simple that they do not require any preliminary separation steps. Accuracy, precision and linearity ranges of these methods were determined. Moreover, specificity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied for analysis of bo...
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2015
This work is concerned with development and validation of three simple, specific, accurate and pr... more This work is concerned with development and validation of three simple, specific, accurate and precise spectrophotometric methods for determination of flumethasone pivalate (FP) and clioquinol (CL) in their binary mixture and ear drops. Method A is a ratio subtraction spectrophotometric one (RSM). Method B is a ratio difference spectrophotometric one (RDSM), while method C is a mean center spectrophotometric one (MCR). The calibration curves are linear over the concentration range of 3-45 μg/mL for FP, and 2-25 μg/mL for CL. The specificity of the developed methods was assessed by analyzing different laboratory prepared mixtures of the FP and CL. The three methods were validated as per ICH guidelines; accuracy, precision and repeatability are found to be within the acceptable limits.
Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitomet... more Simple, accurate, sensitive and validated UV spectrophotometric, chemometric and HPTLC-densitometric methods were developed for determination of oxybutynin hydrochloride (OX) in presence of its degradation product and additives in its pharmaceutical formulations. Method A is the first derivative of ratio spectra (DD(1)) which allows the determination of OX in presence of its degradate in pure form and tablets by measuring the peaks amplitude at 216 nm. Method B and C are principal component regression (PCR) and partial least-squares (PLS) for determination of OX in presence of its degradate in pure form, tablets and syrup. While, the developed high performance thin layer chromatography HPTLC-densitometric method was based on the separation of OX from its degradation product, methylparaben and propylparaben followed by densitometric measurement at 220 nm which allows the determination of OX in pure form, tablets and syrup. The separation was achieved using HPTLC silica gel F(254) plates and chloroform:methanol:ammonia solution:triethylamine (100:3:0.5:0.2, v/v/v/v) as the developing system. The accuracy, precision and linearity ranges of the developed methods were determined. The results obtained were statistically compared with each other and to that of a reported HPLC method, and there was no significant difference between the proposed methods and the reported method regarding both accuracy and precision.
Journal of Dispersion Science and Technology, 2012
In this paper, three deferent surfactants as corrosion inhibitor were prepared in two steps; In t... more In this paper, three deferent surfactants as corrosion inhibitor were prepared in two steps; In the first step, maleic anhydride was amidated with dodecylamine to produce polyoxy ethylenyl-2-ene-4-dodecanamide butanoic acid. In the second step, the resulting product was further was further esterified with different molecular weights of polyethylene glycol (m. wt. = 200, 400 and 600) namely; MP2, MP4 and MP6. The
Norfloxacin (Nor), (1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7 (1-piperazinyl)-3-quinoline carboxylic a... more Norfloxacin (Nor), (1-ethyl-6-fluoro-1,4-dihydro-4-oxo-7 (1-piperazinyl)-3-quinoline carboxylic acid is a synthetic an-tibacterial drug.1,2) Methods were reported for its determina-tion as titrimetric,3) LC,410) LC-MS,1113) spectrometric,1416) spectrofluorimetric1720) ...
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Jan 8, 2015
Smart spectrophotometric methods have been applied and validated for the simultaneous determinati... more Smart spectrophotometric methods have been applied and validated for the simultaneous determination of a binary mixture of chloramphenicol (CPL) and prednisolone acetate (PA) without preliminary separation. Two novel methods have been developed; the first method depends upon advanced absorbance subtraction (AAS), while the other method relies on advanced amplitude modulation (AAM); in addition to the well established dual wavelength (DW), ratio difference (RD) and constant center coupled with spectrum subtraction (CC-SS) methods. Accuracy, precision and linearity ranges of these methods were determined. Moreover, selectivity was assessed by analyzing synthetic mixtures of both drugs. The proposed methods were successfully applied to the assay of drugs in their pharmaceutical formulations. No interference was observed from common additives and the validity of the methods was tested. The obtained results have been statistically compared to that of official spectrophotometric methods t...
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