Malignant cells require a large amount of iron for cell growth. Transferrin transfers iron from b... more Malignant cells require a large amount of iron for cell growth. Transferrin transfers iron from blood to cells by reacting with transferrin receptor (TfR). In humans, transferrin and transferrin receptor correlate with oral squamous cell carcinoma (OSCC); however, the association with canine oral tumor has not been studied. This study aimed to investigate saliva and serum transferrin and tissue transferrin receptor in canine OSCC and oral melanoma (OM). Saliva and serum transferrin samples were collected pre- and 14 days post-operation, compared with healthy control and evaluated by a sandwich ELISA assay. Serum transferrin concentration in the experimental group both before and after surgery was significantly lower than that in the normal group (p < 0.05). However, no significant difference in serum transferrin levels before and after surgery was observed. Saliva transferrin expression of the dogs with oronasal cancer before surgical treatment tended to be higher than that of th...
The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-P... more The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa were 85% (17/20), 58% (11/19) and 40% (6/15), respectively. In addition, the positive detection of amplified NP gene by RT-PCR from the brain, lungs, and gastrointestinal tissues were 100% (18/18), 83% (15/18) and 75% (12/16), respectively. Necropsy diagnosis made by histopathology and immunoh...
The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department ... more The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department of Surgery, Small Animal Hospital, Chulalongkorn University, between November 2000 and November 2003, in relation to breed, sex, age, location, tumor size, histopathological diagnosis and surgical treatment is reported. The average age of the affected dogs was 7.85 years. Mixed breeds, males and the upper eyelid were at a higher risk. The average size of the mass was 6.49 mm. Benign tumors (88.41%) were more frequent than malignant ones (11.59%). Sebaceous gland tumors were more frequently encounted (79.7%) than malignant tumors, malignant melanomas, squamous cell carcinomas and mastocytomas. Amputation and liquid nitrogen spray cryotherapy is the effective treatment for all eyelid masses with a 100% cure rate.
Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns ... more Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A few acid fast bacilli were found within the multinucleated giant cells at the periphery of the granulomas. Mycobacterium tuberculosis was identified from affected organs using specific Rv1970 PCR primer. The aim of this study is to report a rare case of disseminated tuberculosis caused by M. tuberculosis ...
Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were am... more Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the result of the introduction of a new variant into the endemic area or by local evolution.
Songklanakarin Journal of Science and Technology, 2010
Canine distemper is a highly contagious and immunosuppressive viral disease caused by canine dist... more Canine distemper is a highly contagious and immunosuppressive viral disease caused by canine distemper virus (CDV), an enveloped RNA virus of the family Paramyxoviridae. The susceptible host spectrum of CDV is broad and includes all families of the order Carnivora. To accomplish the infection, CDV requires an expression of signaling lymphocyte activation molecule (SLAM) functioning as a cellular receptor which generally presents in a variety of different lymphoid cell subpopulations, including immature thymocytes, primary B cells, activated T cells, memory T cells, macrophages and mature dendritic cells. The distribution of SLAM-presenting cells is in accordance with the lymphotropism and immunosuppression following morbillivirus infection. In the present study, the C57BL/6 mice engrafted with dog-specific SLAM sequence (DogSLAM) were used. The weanling (3-week-old) transgenic offspring C57BL/6 mice were infected with CDV Snyder Hill (CDV-SH) strain via the intranasal (n=6), intrace...
The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development ... more The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was found in 17/60 cases (28.3%); 3/15 (20%) of SCC, 3/15 (20%) of basal cell tumors (BCT) and 2/5 (40%) of apocrine gland tumors (AGT). Positive cytoplasmic staining for c-yes protein was detected in 16/60 cases (26.7%); 1/15 (6.7%) of SCC and 7/15 (46.7%) of BCT. The expression of these onco...
The study looked at the relationship between the expression of c-erbB2 oncogene protein, histolog... more The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade I...
Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was suc... more Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was successfully carried out by amputation of the nictitan. No recurrence of the mass or pulmonary metastasis was evident within a period of twelve months after the treatment.
An 8 year-old male mongrel dog and 12 year-old male German Shepherd had a 10 x 10 cm mass and 15 ... more An 8 year-old male mongrel dog and 12 year-old male German Shepherd had a 10 x 10 cm mass and 15 x 10 cm mass located in the mandibular region. The encapsulated firm white masses had multiple cysticlike nodules filled with mucous. Histopathology revealed clusters of pleomorphic, large, round to oval shaped cells, irregular ovoid to spindle nuclei with highly mitotic figures that showed osteoid and bony formation. The neoplastic cells were immunoreactive in some parts for vimentin and cytokeratin but not for desmin, α-SMA and S-100 protein. Hyperplastic salivary gland cells were also present. Electron microscopy revealed prominent nucleoli with an abundant, dilated, rough, endoplasmic reticulum. A final diagnosis of extraskeletal osteosarcoma originating from interstitial cells of the salivary gland was made.
Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting bo... more Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the neoplastic cell revealed typical features of SCC and immunoreactive to cytokeratin in the cytoplasm of the neoplastic cells. This case report is the first to describe a well-differentiated SCC in a clouded leopard.
Skin biopsied tissues from two dogs, initially diagnosed as hemophagocytic histiocytic sarcoma (H... more Skin biopsied tissues from two dogs, initially diagnosed as hemophagocytic histiocytic sarcoma (HHS) by histopathology, were immunostained with a series of antibodies against lambda light chains, kappa light chains, multiple myeloma oncoprotein 1 (MUM-1), CD-3, paired box 5 (PAX-5), E-cadherin and lysozyme. Heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) was additionally performed on formalin-fixed paraffin-embedded (FFPE) tissues to rule out lymphomas from histiocytomas. Results showed that both cases were immunopositive for lambda light chains, MUM-1, PAK-5 and lysozyme, indicating a diagnosis of extramedullary plasmacytoma (EMP). Moreover, negative PCR result provided additional evidence confirming the diagnosis. Even though hPARR could not differentiate between histiocytomas and EMP, it could rule out these tumors as malignant lymphomas. This report emphasizes the advantage of using a panel of antibodies, besides histopathology, for HHS diagnosis.
Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisi... more Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed...
A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, h... more A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, high fever, dysuria, nasal discharge and moist rale lung sounds. Gross examination revealed a single, white, firm, pituitary mass with a diameter 2.5 x 2.1 x 1.5 cm, compressed and overlying the hypothalamus. Histopathological and immunohistochemical examinations revealed the characteristic of a ACTH-derived pituitary adenoma.
The purpose of this paper is to evaluate the relationship between Melan A protein expression, his... more The purpose of this paper is to evaluate the relationship between Melan A protein expression, histopathological appearance, clinical data and proliferative marker indices for the diagnosis and prognosis of canine oral melanomas. A retrospective study was performed on biopsy specimens of 65 dogs submitted during the years of 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results revealed that Melan A protein expression was detected in 50.77% (33/65) malignant melanomas. The results did not show any significant differences between Melan A protein expression and histopathological classification, clinical data, average AgNORs and PCNA indices. Melan A expression could be a useful marker for melanocytic derivation diagnosis in dogs. The study indicated that the expression of Melan A protein in combination with AgNORs and PCNA indices could be used to provide a prognosis for oral melanoma in dogs.
BackgroundVarious types of oral tumors, either benign or malignant, are commonly found in dogs. S... more BackgroundVarious types of oral tumors, either benign or malignant, are commonly found in dogs. Since saliva directly contacts the tumors and saliva collection is non-invasive, easily accessible and cost effective, salivary biomarkers are practical to be used for the diagnosis and/or prognosis of these diseases. However, there is limited knowledge of protein expression in saliva for canine oral tumors. The present study aimed to investigate novel biomarkers from the salivary proteome of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP), using an in-gel digestion coupled with mass spectrometry (GeLC-MS/MS). The relationships between protein candidates and chemotherapy drugs were explored and the expression of potential biomarkers in saliva and tissues was verified by western blot analysis.ResultsFor saliva samples, increased expression of protein tyrosine...
Background Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubul... more Background Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubulointerstitial nephritis, but FeMV is also detected in healthy cats. This research aimed to identify and characterize the FeMV strains detected in a Thai cat population. Results Two-hundred and ninety-two samples (131 urine and 161 blood) derived from 261 cats (61 sheltered and 200 household cats) were included for investigating the FeMV prevalence using real-time reverse transcription PCR. The overall prevalence of FeMV detection was 11.9% (31/261) among both samples, which accounted for 14.5% (19/131) and 7.5% (12/161) of the urine and blood samples, respectively. Among the FeMV-PCR positive cats, the FeMV-detected prevalence was insignificantly associated with healthy cats (58.1%; 18/31) or urologic cats (41.9%; 13/31). Full-length genome analysis of these FeMV-Thai strains revealed that their genomes clustered together in the FeMV-1A clade with up to 98.5% nucleotide identity. Selecti...
The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression o... more The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression of drug resistant and anti-apoptotic proteins in tumor cells. In a rescue protocol we evaluated the ability of toceranib phosphate concurrent with lomustine (CCNU) or L-asparaginase and vincristine to decrease drug resistant and apoptotic proteins in relapsed and refractory canine lymphomas. The peripheral blood samples were collected before and after the rescue treatment from fourteen dogs that were refractory to cyclophosphamide-vincristine-prednisolone (COP) or COP-doxorubicin (CHOP) treatment and had recurrent multicentric lymphoma. The mRNA expression level of ABCB1, ABCG2, Bcl2 and Bax were determined by quantitative real-time PCR. The fold-change in ABCB1, ABCG2, Bcl2 and Bax mRNA levels were analyzed in correlation with the progression-free survival (PFS). After the rescue treatment, the ABCB1 and ABCG2 mRNA expression levels were 1.57- and 1.85-fold lower (p = 0.4 and p = 0.87), ...
Malignant cells require a large amount of iron for cell growth. Transferrin transfers iron from b... more Malignant cells require a large amount of iron for cell growth. Transferrin transfers iron from blood to cells by reacting with transferrin receptor (TfR). In humans, transferrin and transferrin receptor correlate with oral squamous cell carcinoma (OSCC); however, the association with canine oral tumor has not been studied. This study aimed to investigate saliva and serum transferrin and tissue transferrin receptor in canine OSCC and oral melanoma (OM). Saliva and serum transferrin samples were collected pre- and 14 days post-operation, compared with healthy control and evaluated by a sandwich ELISA assay. Serum transferrin concentration in the experimental group both before and after surgery was significantly lower than that in the normal group (p < 0.05). However, no significant difference in serum transferrin levels before and after surgery was observed. Saliva transferrin expression of the dogs with oronasal cancer before surgical treatment tended to be higher than that of th...
The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-P... more The diagnosis of canine distemper (CD) by a reverse transcriptase polymerase chain reaction (RT-PCR) technique using 2 sets of primer which were specific to two regions on the cDNA of nucleocapsid protein (NP) gene of the canine distemper virus (CDV), Onderstepoort strain. Together with pathology and immunohistochemistry, the RT-PCR test was used to confirm the diagnosis of canine distemper in 20 dogs. Histopathological examination revealed that 70% (14/20) had pneumonia, 50% (10/20) had non-suppurative encephalitis, 33% (7/20) had splenic follicular necrosis and 35% (7/ 20) had gastroenteritis. Immunohistochemical detection of CDV antigens in the brain, lungs, and the gastrointestinal mucosa were 85% (17/20), 58% (11/19) and 40% (6/15), respectively. In addition, the positive detection of amplified NP gene by RT-PCR from the brain, lungs, and gastrointestinal tissues were 100% (18/18), 83% (15/18) and 75% (12/16), respectively. Necropsy diagnosis made by histopathology and immunoh...
The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department ... more The prevalence and treatment of 69 eyelid masses in dogs at the Ophthalmology clinic, Department of Surgery, Small Animal Hospital, Chulalongkorn University, between November 2000 and November 2003, in relation to breed, sex, age, location, tumor size, histopathological diagnosis and surgical treatment is reported. The average age of the affected dogs was 7.85 years. Mixed breeds, males and the upper eyelid were at a higher risk. The average size of the mass was 6.49 mm. Benign tumors (88.41%) were more frequent than malignant ones (11.59%). Sebaceous gland tumors were more frequently encounted (79.7%) than malignant tumors, malignant melanomas, squamous cell carcinomas and mastocytomas. Amputation and liquid nitrogen spray cryotherapy is the effective treatment for all eyelid masses with a 100% cure rate.
Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns ... more Tuberculosis caused by Mycobacterium tuberculosis is an important zoonotic disease that concerns the survival of endangered species and public health aspects. A 17-year-old, female Malayan tapir from a zoo in Thailand died with a sign of panting after anesthesia for the treatment of her oral problems. Grossly, multiple caseous white nodules were scattered throughout the parenchyma and bulged from the capsular surface in affected organs such as lung, lymph node and liver. Histologic examination revealed multifocal to coalescing granulomas characterized by caseous necrotic center, loosely organized inflammatory zone with low numbers of Langhans’s giant cells and thin fibrotic zone. A few acid fast bacilli were found within the multinucleated giant cells at the periphery of the granulomas. Mycobacterium tuberculosis was identified from affected organs using specific Rv1970 PCR primer. The aim of this study is to report a rare case of disseminated tuberculosis caused by M. tuberculosis ...
Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were am... more Ten selected Thai isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) were amplified in an open reading frame 5 by a reverse, transcriptase, polymerase chain reaction (RT-PCR) and cut with restriction enzymes: MluI, HincII, SacII and HaeIII for the US genotype and PstI, HaeII and ClaI for the EU genotype. None of the field isolates had a similar cutting pattern when compared to modified live virus vaccines, however, several cutting patterns were obtained from this study. The results suggested that genetic variation was present among the Thai isolates, even within the same genotype. The variations may be the result of the introduction of a new variant into the endemic area or by local evolution.
Songklanakarin Journal of Science and Technology, 2010
Canine distemper is a highly contagious and immunosuppressive viral disease caused by canine dist... more Canine distemper is a highly contagious and immunosuppressive viral disease caused by canine distemper virus (CDV), an enveloped RNA virus of the family Paramyxoviridae. The susceptible host spectrum of CDV is broad and includes all families of the order Carnivora. To accomplish the infection, CDV requires an expression of signaling lymphocyte activation molecule (SLAM) functioning as a cellular receptor which generally presents in a variety of different lymphoid cell subpopulations, including immature thymocytes, primary B cells, activated T cells, memory T cells, macrophages and mature dendritic cells. The distribution of SLAM-presenting cells is in accordance with the lymphotropism and immunosuppression following morbillivirus infection. In the present study, the C57BL/6 mice engrafted with dog-specific SLAM sequence (DogSLAM) were used. The weanling (3-week-old) transgenic offspring C57BL/6 mice were infected with CDV Snyder Hill (CDV-SH) strain via the intranasal (n=6), intrace...
The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development ... more The exact role of the p53 tumor suppressor gene, c-erbB-2 and c-yes oncogenes in the development of canine skin tumors is still debatable. To elucidate the involvement of these oncogenes in canine skin carcinogenesis, we examined the expression of the protein in 60 skin tumor samples using avidin-biotin complex immunohistochemistry, with polyclonal antibodies directed against each protein, on formalinfixed, paraffin-embedded sections. Positive nuclear staining for p53 protein was observed in 12/60 cases (20%) which included, 6/15(40%) squamous cell carcinomas (SCC) and 4/8(50%) perianal gland tumors (PGT). Out of all the tumors examined, mixed membrane/ cytoplasmic staining of c-erbB-2 protein was found in 17/60 cases (28.3%); 3/15 (20%) of SCC, 3/15 (20%) of basal cell tumors (BCT) and 2/5 (40%) of apocrine gland tumors (AGT). Positive cytoplasmic staining for c-yes protein was detected in 16/60 cases (26.7%); 1/15 (6.7%) of SCC and 7/15 (46.7%) of BCT. The expression of these onco...
The study looked at the relationship between the expression of c-erbB2 oncogene protein, histolog... more The study looked at the relationship between the expression of c-erbB2 oncogene protein, histological types, PCNA and AgNORs indices, in feline mammary tumors. Sixty, surgically resected, mammary tumors from cats were collected and histological processed. The expression of c-erbB2 oncogene protein was detected by immunohistochemistry and was seen in benign hyperplasia, adenosis and malignant groups. The c-erbB2 oncogene protein expression in malignant, feline mammary tumors was 41.67% (25/60) which could be divided into simple tubular ACC, 25% (6/24), simple papillary ACC, 78.6% (11/14), solid carcinoma, 26.7% (4/15) and cribiform carcinoma, 57.14% (4/7). There were statistically significantly differences (p<0.05) in the expression of c-erbB2 oncogene protein between simple tubular ACC, compared with simple papillary ACC and solid carcinomas. The expression of c-erbB2 oncogene protein in specimens, classified by histological malignancy grades revealed, grade I, 42.11% and grade I...
Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was suc... more Hemangiosarcoma of the nictitating membrane of two Great Dane dogs is reported. Treatment was successfully carried out by amputation of the nictitan. No recurrence of the mass or pulmonary metastasis was evident within a period of twelve months after the treatment.
An 8 year-old male mongrel dog and 12 year-old male German Shepherd had a 10 x 10 cm mass and 15 ... more An 8 year-old male mongrel dog and 12 year-old male German Shepherd had a 10 x 10 cm mass and 15 x 10 cm mass located in the mandibular region. The encapsulated firm white masses had multiple cysticlike nodules filled with mucous. Histopathology revealed clusters of pleomorphic, large, round to oval shaped cells, irregular ovoid to spindle nuclei with highly mitotic figures that showed osteoid and bony formation. The neoplastic cells were immunoreactive in some parts for vimentin and cytokeratin but not for desmin, α-SMA and S-100 protein. Hyperplastic salivary gland cells were also present. Electron microscopy revealed prominent nucleoli with an abundant, dilated, rough, endoplasmic reticulum. A final diagnosis of extraskeletal osteosarcoma originating from interstitial cells of the salivary gland was made.
Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting bo... more Squamous cell carcinoma (SCC) is a malignant neoplasm of keratinized epithelial cell affecting both domestic and wild animals. A few cases of SCC have been reported in wild felids. A fifteen year old, intact female, clouded leopard showed signs of lameness and swelling of the right hind paw, which upon physical examination was noted to contain an abscess. Surgical biopsy of the site revealed a well-differentiated SCC. Attempts at surgical excision and cryosurgery proved unsuccessful and there was a recurrence of the mass. Therefore, mid-femoral amputation was performed. Macroscopically, the amputated limb revealed multiple dermal, cavitated whitish masses. Microscopically, the neoplastic cell revealed typical features of SCC and immunoreactive to cytokeratin in the cytoplasm of the neoplastic cells. This case report is the first to describe a well-differentiated SCC in a clouded leopard.
Skin biopsied tissues from two dogs, initially diagnosed as hemophagocytic histiocytic sarcoma (H... more Skin biopsied tissues from two dogs, initially diagnosed as hemophagocytic histiocytic sarcoma (HHS) by histopathology, were immunostained with a series of antibodies against lambda light chains, kappa light chains, multiple myeloma oncoprotein 1 (MUM-1), CD-3, paired box 5 (PAX-5), E-cadherin and lysozyme. Heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) was additionally performed on formalin-fixed paraffin-embedded (FFPE) tissues to rule out lymphomas from histiocytomas. Results showed that both cases were immunopositive for lambda light chains, MUM-1, PAK-5 and lysozyme, indicating a diagnosis of extramedullary plasmacytoma (EMP). Moreover, negative PCR result provided additional evidence confirming the diagnosis. Even though hPARR could not differentiate between histiocytomas and EMP, it could rule out these tumors as malignant lymphomas. This report emphasizes the advantage of using a panel of antibodies, besides histopathology, for HHS diagnosis.
Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisi... more Moringa oleifera Lam (horseradish tree; tender pod or fruits) is a major ingredient in Thai cuisine and has some medicinal properties. Previous studies have shown potentially antioxidant, antitumor promoter, anticlastogen and anticarcinogen activities both in vitro and in vivo. The present study was conducted to investigate chemopreventive effects on azoxymethane (AOM)-initiated and dextran sodium sulfate (DSS)-promoted colon carcinogenesis in mice. Male ICR mice were divided into 8 groups: Group 1 served as a negative control; Group 2 received AOM/DSS as a positive control; Groups 3-5 were fed boiled freeze-dried M. oleifera (bMO) at 1.5%, 3.0% and 6.0%, respectively supplemented in basal diets for 5 weeks; Groups 6-8 were fed with bMO diets at the designed doses above for 2 weeks prior to AOM, during and 1 week after DSS administration. At the end of the study, colon samples were processed for histopathological examination. PCNA indices, and iNOS and COX-2 expression were assessed...
A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, h... more A female, grey, 24-year-old horse was showing clinical signs of weight loss, ataxia, hersutism, high fever, dysuria, nasal discharge and moist rale lung sounds. Gross examination revealed a single, white, firm, pituitary mass with a diameter 2.5 x 2.1 x 1.5 cm, compressed and overlying the hypothalamus. Histopathological and immunohistochemical examinations revealed the characteristic of a ACTH-derived pituitary adenoma.
The purpose of this paper is to evaluate the relationship between Melan A protein expression, his... more The purpose of this paper is to evaluate the relationship between Melan A protein expression, histopathological appearance, clinical data and proliferative marker indices for the diagnosis and prognosis of canine oral melanomas. A retrospective study was performed on biopsy specimens of 65 dogs submitted during the years of 2000 to 2003 to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University. Our results revealed that Melan A protein expression was detected in 50.77% (33/65) malignant melanomas. The results did not show any significant differences between Melan A protein expression and histopathological classification, clinical data, average AgNORs and PCNA indices. Melan A expression could be a useful marker for melanocytic derivation diagnosis in dogs. The study indicated that the expression of Melan A protein in combination with AgNORs and PCNA indices could be used to provide a prognosis for oral melanoma in dogs.
BackgroundVarious types of oral tumors, either benign or malignant, are commonly found in dogs. S... more BackgroundVarious types of oral tumors, either benign or malignant, are commonly found in dogs. Since saliva directly contacts the tumors and saliva collection is non-invasive, easily accessible and cost effective, salivary biomarkers are practical to be used for the diagnosis and/or prognosis of these diseases. However, there is limited knowledge of protein expression in saliva for canine oral tumors. The present study aimed to investigate novel biomarkers from the salivary proteome of dogs with early- and late-stage oral melanoma (EOM and LOM, respectively), oral squamous cell carcinoma (OSCC), benign oral tumors (BN), and periodontitis and healthy controls (CP), using an in-gel digestion coupled with mass spectrometry (GeLC-MS/MS). The relationships between protein candidates and chemotherapy drugs were explored and the expression of potential biomarkers in saliva and tissues was verified by western blot analysis.ResultsFor saliva samples, increased expression of protein tyrosine...
Background Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubul... more Background Feline morbillivirus (FeMV) has been discovered in domestic cats associated with tubulointerstitial nephritis, but FeMV is also detected in healthy cats. This research aimed to identify and characterize the FeMV strains detected in a Thai cat population. Results Two-hundred and ninety-two samples (131 urine and 161 blood) derived from 261 cats (61 sheltered and 200 household cats) were included for investigating the FeMV prevalence using real-time reverse transcription PCR. The overall prevalence of FeMV detection was 11.9% (31/261) among both samples, which accounted for 14.5% (19/131) and 7.5% (12/161) of the urine and blood samples, respectively. Among the FeMV-PCR positive cats, the FeMV-detected prevalence was insignificantly associated with healthy cats (58.1%; 18/31) or urologic cats (41.9%; 13/31). Full-length genome analysis of these FeMV-Thai strains revealed that their genomes clustered together in the FeMV-1A clade with up to 98.5% nucleotide identity. Selecti...
The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression o... more The main problems that cause unresponsiveness to an anti-neoplastic drug are the overexpression of drug resistant and anti-apoptotic proteins in tumor cells. In a rescue protocol we evaluated the ability of toceranib phosphate concurrent with lomustine (CCNU) or L-asparaginase and vincristine to decrease drug resistant and apoptotic proteins in relapsed and refractory canine lymphomas. The peripheral blood samples were collected before and after the rescue treatment from fourteen dogs that were refractory to cyclophosphamide-vincristine-prednisolone (COP) or COP-doxorubicin (CHOP) treatment and had recurrent multicentric lymphoma. The mRNA expression level of ABCB1, ABCG2, Bcl2 and Bax were determined by quantitative real-time PCR. The fold-change in ABCB1, ABCG2, Bcl2 and Bax mRNA levels were analyzed in correlation with the progression-free survival (PFS). After the rescue treatment, the ABCB1 and ABCG2 mRNA expression levels were 1.57- and 1.85-fold lower (p = 0.4 and p = 0.87), ...
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