Acinetobacter baumannii is a Gram-negative bacterium increasingly implicated in hospital-acquired... more Acinetobacter baumannii is a Gram-negative bacterium increasingly implicated in hospital-acquired infections and outbreaks. Effective prevention and control of such infections are commonly challenged by the frequent emergence of multidrug-resistant strains. Here we introduce Ab-web (https://www.acinetobacterbaumannii.no), the first online platform for sharing expertise on A. baumannii. Ab-web is a species-centric knowledge hub, initially with 10 articles organized into two main sections, ‘Overview’ and ‘Topics’, and three themes, ‘epidemiology’, ‘antibiotic resistance’, and ‘virulence’. The ‘workspace’ section provides a spot for colleagues to collaborate, build, and manage joint projects. Ab-web is a community-driven initiative amenable to constructive feedback and new ideas.
Introduction: Many people worldwide attempt to lose weight or adopt strategies to control it. Som... more Introduction: Many people worldwide attempt to lose weight or adopt strategies to control it. Some have resorted to the consumption of commercialized diet pills to achieve this goal. Multiple brands exist without clearly indicating their mechanism of action or adverse effects on human health. This study aims to determine the antibacterial effect of commercial diet pills on members of the intestinal microbiota. Methodology: Commercialized diet pills were bought from a pharmacy in the North of Lebanon. Broth microdilution test was performed to determine the Minimum Inhibitory Concentrations (MICs) of the aqueous suspension against forty-two isolates distributed into four Enterobacterales species. MIC of the digested form was determined against six different strains. GC-MS analysis was performed to elucidate the components of the diet pill compared to the manufacturer's list. Results: Broth microdilution results revealed that MICs of the diet pill aqueous suspension ranged from 3.9...
Poultry are currently regarded as reservoirs from which multidrug resistance can be readily trans... more Poultry are currently regarded as reservoirs from which multidrug resistance can be readily transferred to the surrounding ecosystem. The aim of this study was to explore the prevalence of extended spectrum beta-lactamases (ESBLs) and ampC and mcr-1 Gram-negative bacilli in chicken, farmers, and environment in Lebanon. In May 2017, we revisited the farm where the first mcr-1 was detected in 2015 in Lebanon. Overall 200 chicken fecal swabs, 6 farmers' fecal samples, and 41 environmental samples were collected. Real time (RT)-PCR was performed for beta-lactamases and mcr genes' screening using newly designed primers/probes. Multilocus sequence typing (MLST) was also performed. ESBL/ampCs were found in 118 samples from chicken, 4 from workers, and all environmental ones. mcr-1 was detected in all chicken and farmers' samples from which 314 and 7 strains were isolated, respectively. Three mcr-1 Escherichia coli strains were isolated from litter and feed. Compared to 2015, the prevalence of ESBL/ampC producers, TEM and CTX-M genes increased significantly in 2017. Main spectra profiles dendrogram of isolated E. coli strains in 2015/2017 and MLST revealed the presence of different clones and sequence types. The evolution of resistance appears to be multi-clonal and related to the diffusion of plasmids carrying ESBL and mcr-1 genes. More work is needed to quantify the magnitude of this emerging problem in Lebanon.
Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union,... more Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 114 kb)
Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based... more Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 113 kb)
This table represents all the bacterial species isolated at least once from the human being, clas... more This table represents all the bacterial species isolated at least once from the human being, classified according to its phylum and combining the present work and the previous species reported by Hugon et al., in 2015. (XLSX 73 kb)
Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union,... more Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 114 kb)
Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based... more Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 113 kb)
This table represents all the bacterial species isolated at least once from the human being, clas... more This table represents all the bacterial species isolated at least once from the human being, classified according to its phylum and combining the present work and the previous species reported by Hugon et al., in 2015. (XLSX 73 kb)
Acinetobacter baumannii is a Gram-negative bacterium increasingly implicated in hospital-acquired... more Acinetobacter baumannii is a Gram-negative bacterium increasingly implicated in hospital-acquired infections and outbreaks. Effective prevention and control of such infections are commonly challenged by the frequent emergence of multidrug-resistant strains. Here we introduce Ab-web (https://www.acinetobacterbaumannii.no), the first online platform for sharing expertise on A. baumannii. Ab-web is a species-centric knowledge hub, initially with 10 articles organized into two main sections, ‘Overview’ and ‘Topics’, and three themes, ‘epidemiology’, ‘antibiotic resistance’, and ‘virulence’. The ‘workspace’ section provides a spot for colleagues to collaborate, build, and manage joint projects. Ab-web is a community-driven initiative amenable to constructive feedback and new ideas.
Introduction: Many people worldwide attempt to lose weight or adopt strategies to control it. Som... more Introduction: Many people worldwide attempt to lose weight or adopt strategies to control it. Some have resorted to the consumption of commercialized diet pills to achieve this goal. Multiple brands exist without clearly indicating their mechanism of action or adverse effects on human health. This study aims to determine the antibacterial effect of commercial diet pills on members of the intestinal microbiota. Methodology: Commercialized diet pills were bought from a pharmacy in the North of Lebanon. Broth microdilution test was performed to determine the Minimum Inhibitory Concentrations (MICs) of the aqueous suspension against forty-two isolates distributed into four Enterobacterales species. MIC of the digested form was determined against six different strains. GC-MS analysis was performed to elucidate the components of the diet pill compared to the manufacturer's list. Results: Broth microdilution results revealed that MICs of the diet pill aqueous suspension ranged from 3.9...
Poultry are currently regarded as reservoirs from which multidrug resistance can be readily trans... more Poultry are currently regarded as reservoirs from which multidrug resistance can be readily transferred to the surrounding ecosystem. The aim of this study was to explore the prevalence of extended spectrum beta-lactamases (ESBLs) and ampC and mcr-1 Gram-negative bacilli in chicken, farmers, and environment in Lebanon. In May 2017, we revisited the farm where the first mcr-1 was detected in 2015 in Lebanon. Overall 200 chicken fecal swabs, 6 farmers' fecal samples, and 41 environmental samples were collected. Real time (RT)-PCR was performed for beta-lactamases and mcr genes' screening using newly designed primers/probes. Multilocus sequence typing (MLST) was also performed. ESBL/ampCs were found in 118 samples from chicken, 4 from workers, and all environmental ones. mcr-1 was detected in all chicken and farmers' samples from which 314 and 7 strains were isolated, respectively. Three mcr-1 Escherichia coli strains were isolated from litter and feed. Compared to 2015, the prevalence of ESBL/ampC producers, TEM and CTX-M genes increased significantly in 2017. Main spectra profiles dendrogram of isolated E. coli strains in 2015/2017 and MLST revealed the presence of different clones and sequence types. The evolution of resistance appears to be multi-clonal and related to the diffusion of plasmids carrying ESBL and mcr-1 genes. More work is needed to quantify the magnitude of this emerging problem in Lebanon.
Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union,... more Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 114 kb)
Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based... more Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 113 kb)
This table represents all the bacterial species isolated at least once from the human being, clas... more This table represents all the bacterial species isolated at least once from the human being, classified according to its phylum and combining the present work and the previous species reported by Hugon et al., in 2015. (XLSX 73 kb)
Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union,... more Table S1. P. aeruginosa percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 114 kb)
Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based... more Table S1. E. coli percent susceptibility to carbapenems in countries of the European Union, based on the 2015 and 2016 annual reports of the European Antimicrobial Resistance Surveillance Network (EARS-Net)1,2, and comparison to 2015â 2016 Lebanese data. (DOCX 113 kb)
This table represents all the bacterial species isolated at least once from the human being, clas... more This table represents all the bacterial species isolated at least once from the human being, classified according to its phylum and combining the present work and the previous species reported by Hugon et al., in 2015. (XLSX 73 kb)
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