The aim of the present study is to evaluate the effect of aqueous extract of Thonningia sanguinea... more The aim of the present study is to evaluate the effect of aqueous extract of Thonningia sanguinea on blood electrolytes of broiler chickens. To do this, two hundred (200) 15-day-old Hubbard broiler chickens were divided into 2 batches of 100 chickens each. The chickens in the control group (Batch C) received unsupplemented water while those in the test group (Batch E) received water supplemented with 10 g / L of aqueous extract of T. sanguinea for one week. Then, the zootechnical parameters such as weight growth, weight gain, consumption index and the level of serum electrolytes were determined. The results of the study showed that the aqueous extract of Thonningia sanguinea improved weight growth, weight gain and consumption index. At the same time, the results also showed that T. sanguinea did not significantly (p <0.05) affect the level of blood electrolytes of broilers chickens. From the results obtained in the present study, the aqueous extract of Thonningia sanguinea could ...
BACKGROUND The aetiologies of fever are poorly understood in sub-Saharan Africa. We aimed to asse... more BACKGROUND The aetiologies of fever are poorly understood in sub-Saharan Africa. We aimed to assess the burden of malaria and bacteria in Côte d'Ivoire. METHODS Blood samples from 438 febrile and 346 afebrile people were screened using molecular tools. RESULTS Plasmodium falciparum was the most common microorganism associated with fever (46.8% in febrile; 23.4% in afebrile people p<0.001). Bacteraemia was detected in 21.7% of febrile people and 12.7% of afebrile people (p=0.001). Streptococcus pneumoniae was the main cause of bacteraemia (7.1% of febrile and 0.6% of afebrile individuals; p<0.001). Non-typhoidal Salmonella spp. was detected in 4.5% of febrile people and 1.2% of afebrile individuals (p<0.001). Salmonella enterica Typhi and S. enterica Paratyphi were only detected in febrile subjects (1.4% and 2.1%), as well as Tropheryma whipplei (0.9%), Streptococcus pyogenes (0.7%), and Plasmodium ovale (4.6%). The prevalence in febrile and afebrile people was similar for Staphylococcus aureus (3.6-4.9%), Rickettsia felis (5.5-6.4%), Mansonella perstans (3.0-3.2%), and Plasmodium malariae (1.6-2.3%). Comorbidities were higher in febrile than in afebrile subjects (10.3% versus 5.5%; p=0.01); 82% involving P. falciparum. All patients co-infected with P. falciparum and S. pneumoniae were febrile whereas 30.0% of those infected by P. falciparum alone were not (p=0.02). Among febrile participants, 30.4% with malaria and 54.7% with bacteraemia had received neither antimalarial nor antibiotic therapy. CONCLUSION Identification of etiologies of acute febrile diseases in sub-Saharan Africa proposes keys to successful treatment and prevention of infectious diseases. Vaccination campaigns may decrease the morbidity of mono- and co-infections by preventable microorganisms.
This study aims to highlight the anti-inflammatory activity of ethanol extract of Annona senegale... more This study aims to highlight the anti-inflammatory activity of ethanol extract of Annona senegalensis and do its phytochemical screening. Rats were divided into three groups. The first group received only saline injection and instillation by intraperitoneal injection on days D0 and D7. This phase was the sensitization of that group. Then, on days D21, D22 and D23, the rats of the same group (Group 1) were injected with saline under anesthesia. The second group (Group 2) was composed of rats had not undergone treatment with the extract of Annona senegalensis. The rats in this batch have been sensitized by intraperitoneal injection (50 μL) of a solution of albumin (50 mg/rat) dissolved in aluminum hydroxide on days 0 and 7. Then during the challenge phase, saline containing 0.9% sodium chloride were injected intraperitoneally on days D21, D22 and D23. The sacrifice took place at D24 or 24 hours after the last challenge to ovalbumin. Similarly, rats of the third group (Group 3) have been sensitized by ovalbumin combined with aluminum hydroxide on days D0 and day D7. Then during the stage of provocation, rats in this batch received at days D21, D22 and D23, conjugated injection of albumin and ethanol extract of Annona senegalensis (injection of 0.4 mL of 7.10(-2) mg/kg body weight). The aqueous extract of Annona senegalensis has been previously prepared in saline. Twenty four hours after the last injection corresponding to D23, the rats were sacrificed under anesthesia. Secondary metabolites have been characterized by physico-chemical properties. Rats in the control (Group 1) gave an average of 24 ± 0.02 mast cells, 7 ± 0.1 macrophages, 9 ± 0.05 eosinophils. In the control group was not revealed the presence of neutrophils. Following the steps of provocation and awareness albumin (Group 2), we observed a significant increase in the number of inflammatory cells compared to control group (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Indeed, mast cells and macrophages have suffered increased respectively to 164 ± 0.01 and 253 ± 0.04. While eosinophils have increased from 9 ± 0.05 to 81 ± 0.01. There were 31 ± 0.02 neutrophils in Group 2. Group 3 treated with Annona senegalensis (7.10(-2) mg/kg) induced a significant decrease in the number of inflammatory cells compared to control group (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Indeed, mast cells decreased from 164 ± 0.01 to 89 ± 0.03. Similarly, the number of macrophages decreased from 253 ± 0.04 to 175 ± 0.06 and neutrophils decreased from 31 ± 0.02 to 10 ± 0.05. Finally, the eosinophils have suffered a decrease (from 81 ± 0.01 to 61 ± 0.08). However, after treatment with the extract, the values of different cell types have always been significantly higher (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) compared to those in the control group (except neutrophils). This result indicates that the extract of Annona senegalensis did not completely inhibit the inflammatory effect induced by albumin. The major classes of secondary metabolites, terpenoids, coumarins, flavonoids and tannins were detected in the leaves of the plant. However, they are low in alkaloids and substances quinone. The extract induced a significant decrease in the number of inflammatory cells. This effect is likely due to higher concentrations of tannins and phenolic compounds in the extract of plant. Thus this study provides a scientific validation of the use of this plant against asthma and cough in the Ivorian pharmacopoeia. However, its mechanism of action remains to be elucidated.
The present study was conducted to evaluate growth performance, selected biochemical serum parame... more The present study was conducted to evaluate growth performance, selected biochemical serum parameters adding in broiler chickens provide with drinking water containing an aqueous extract of Thonningia sanguinea (THOS). Two hundred 1-days-old broiler chickens were acclimated for two weeks. Then they were randomly divided into two equal groups of 100 chickens. In experimental group, aqueous extracts of T. sanguinea were added to water drinkers (10 g.L-1 water) from day 15 to day 22. Through the experience, body weight, feed consumption, and number of dead birds were recorded once a week. At 15, 22, 29, 36 and 45 days of rearing, 20 birds were randomly selected in each group for serum biochemical profile analysis involving, alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), creatin phospoho-kinase (CPK) and Lactate dehydrogenase (LDH). The analyses reveal that the supplementing of aqueous extract of THOS contributed to increase in body weight (p<0.05), decrease cons...
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, Jan 10, 2008
Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity o... more Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat's isolated heart. In this work we studied the effect of MYTA on microsomal Na(+)/K(+) dependant ATPase (Na(+), K(+) ATPase) extracted from the heart of a rabbit since digitalics inhibit Na(+), K(+) ATPase. Our results revealed that the Na(+)/K(+) ATPase has an optimum pH of 7.4 and temperature of 37 degrees C respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 microM/min. LINEWEAVER and BURK plots showed that MYTA did not alter K(M) (1.31 mM) but decreased V(MAX). This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na(+)/K(+) ATPase extracted from rabbit heart with a Ci(50) of 48 microg/ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na(+)/K(+) AT...
The aim of the present study is to evaluate the effect of aqueous extract of Thonningia sanguinea... more The aim of the present study is to evaluate the effect of aqueous extract of Thonningia sanguinea on blood electrolytes of broiler chickens. To do this, two hundred (200) 15-day-old Hubbard broiler chickens were divided into 2 batches of 100 chickens each. The chickens in the control group (Batch C) received unsupplemented water while those in the test group (Batch E) received water supplemented with 10 g / L of aqueous extract of T. sanguinea for one week. Then, the zootechnical parameters such as weight growth, weight gain, consumption index and the level of serum electrolytes were determined. The results of the study showed that the aqueous extract of Thonningia sanguinea improved weight growth, weight gain and consumption index. At the same time, the results also showed that T. sanguinea did not significantly (p <0.05) affect the level of blood electrolytes of broilers chickens. From the results obtained in the present study, the aqueous extract of Thonningia sanguinea could ...
BACKGROUND The aetiologies of fever are poorly understood in sub-Saharan Africa. We aimed to asse... more BACKGROUND The aetiologies of fever are poorly understood in sub-Saharan Africa. We aimed to assess the burden of malaria and bacteria in Côte d'Ivoire. METHODS Blood samples from 438 febrile and 346 afebrile people were screened using molecular tools. RESULTS Plasmodium falciparum was the most common microorganism associated with fever (46.8% in febrile; 23.4% in afebrile people p<0.001). Bacteraemia was detected in 21.7% of febrile people and 12.7% of afebrile people (p=0.001). Streptococcus pneumoniae was the main cause of bacteraemia (7.1% of febrile and 0.6% of afebrile individuals; p<0.001). Non-typhoidal Salmonella spp. was detected in 4.5% of febrile people and 1.2% of afebrile individuals (p<0.001). Salmonella enterica Typhi and S. enterica Paratyphi were only detected in febrile subjects (1.4% and 2.1%), as well as Tropheryma whipplei (0.9%), Streptococcus pyogenes (0.7%), and Plasmodium ovale (4.6%). The prevalence in febrile and afebrile people was similar for Staphylococcus aureus (3.6-4.9%), Rickettsia felis (5.5-6.4%), Mansonella perstans (3.0-3.2%), and Plasmodium malariae (1.6-2.3%). Comorbidities were higher in febrile than in afebrile subjects (10.3% versus 5.5%; p=0.01); 82% involving P. falciparum. All patients co-infected with P. falciparum and S. pneumoniae were febrile whereas 30.0% of those infected by P. falciparum alone were not (p=0.02). Among febrile participants, 30.4% with malaria and 54.7% with bacteraemia had received neither antimalarial nor antibiotic therapy. CONCLUSION Identification of etiologies of acute febrile diseases in sub-Saharan Africa proposes keys to successful treatment and prevention of infectious diseases. Vaccination campaigns may decrease the morbidity of mono- and co-infections by preventable microorganisms.
This study aims to highlight the anti-inflammatory activity of ethanol extract of Annona senegale... more This study aims to highlight the anti-inflammatory activity of ethanol extract of Annona senegalensis and do its phytochemical screening. Rats were divided into three groups. The first group received only saline injection and instillation by intraperitoneal injection on days D0 and D7. This phase was the sensitization of that group. Then, on days D21, D22 and D23, the rats of the same group (Group 1) were injected with saline under anesthesia. The second group (Group 2) was composed of rats had not undergone treatment with the extract of Annona senegalensis. The rats in this batch have been sensitized by intraperitoneal injection (50 μL) of a solution of albumin (50 mg/rat) dissolved in aluminum hydroxide on days 0 and 7. Then during the challenge phase, saline containing 0.9% sodium chloride were injected intraperitoneally on days D21, D22 and D23. The sacrifice took place at D24 or 24 hours after the last challenge to ovalbumin. Similarly, rats of the third group (Group 3) have been sensitized by ovalbumin combined with aluminum hydroxide on days D0 and day D7. Then during the stage of provocation, rats in this batch received at days D21, D22 and D23, conjugated injection of albumin and ethanol extract of Annona senegalensis (injection of 0.4 mL of 7.10(-2) mg/kg body weight). The aqueous extract of Annona senegalensis has been previously prepared in saline. Twenty four hours after the last injection corresponding to D23, the rats were sacrificed under anesthesia. Secondary metabolites have been characterized by physico-chemical properties. Rats in the control (Group 1) gave an average of 24 ± 0.02 mast cells, 7 ± 0.1 macrophages, 9 ± 0.05 eosinophils. In the control group was not revealed the presence of neutrophils. Following the steps of provocation and awareness albumin (Group 2), we observed a significant increase in the number of inflammatory cells compared to control group (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Indeed, mast cells and macrophages have suffered increased respectively to 164 ± 0.01 and 253 ± 0.04. While eosinophils have increased from 9 ± 0.05 to 81 ± 0.01. There were 31 ± 0.02 neutrophils in Group 2. Group 3 treated with Annona senegalensis (7.10(-2) mg/kg) induced a significant decrease in the number of inflammatory cells compared to control group (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). Indeed, mast cells decreased from 164 ± 0.01 to 89 ± 0.03. Similarly, the number of macrophages decreased from 253 ± 0.04 to 175 ± 0.06 and neutrophils decreased from 31 ± 0.02 to 10 ± 0.05. Finally, the eosinophils have suffered a decrease (from 81 ± 0.01 to 61 ± 0.08). However, after treatment with the extract, the values of different cell types have always been significantly higher (p &amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001) compared to those in the control group (except neutrophils). This result indicates that the extract of Annona senegalensis did not completely inhibit the inflammatory effect induced by albumin. The major classes of secondary metabolites, terpenoids, coumarins, flavonoids and tannins were detected in the leaves of the plant. However, they are low in alkaloids and substances quinone. The extract induced a significant decrease in the number of inflammatory cells. This effect is likely due to higher concentrations of tannins and phenolic compounds in the extract of plant. Thus this study provides a scientific validation of the use of this plant against asthma and cough in the Ivorian pharmacopoeia. However, its mechanism of action remains to be elucidated.
The present study was conducted to evaluate growth performance, selected biochemical serum parame... more The present study was conducted to evaluate growth performance, selected biochemical serum parameters adding in broiler chickens provide with drinking water containing an aqueous extract of Thonningia sanguinea (THOS). Two hundred 1-days-old broiler chickens were acclimated for two weeks. Then they were randomly divided into two equal groups of 100 chickens. In experimental group, aqueous extracts of T. sanguinea were added to water drinkers (10 g.L-1 water) from day 15 to day 22. Through the experience, body weight, feed consumption, and number of dead birds were recorded once a week. At 15, 22, 29, 36 and 45 days of rearing, 20 birds were randomly selected in each group for serum biochemical profile analysis involving, alanine aminotransferase (ALAT), aspartate aminotransferase (ASAT), creatin phospoho-kinase (CPK) and Lactate dehydrogenase (LDH). The analyses reveal that the supplementing of aqueous extract of THOS contributed to increase in body weight (p<0.05), decrease cons...
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, Jan 10, 2008
Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity o... more Mitragyna ciliata (MYTA) (Rubiaceae) inhibits plasmodia activity. MYTA induces a cardiotonicity of the digitalic type on rat's isolated heart. In this work we studied the effect of MYTA on microsomal Na(+)/K(+) dependant ATPase (Na(+), K(+) ATPase) extracted from the heart of a rabbit since digitalics inhibit Na(+), K(+) ATPase. Our results revealed that the Na(+)/K(+) ATPase has an optimum pH of 7.4 and temperature of 37 degrees C respectively. There is a linear relationship between the organic phosphate formed and the incubation time over 25 mins incubation period. The ATP hydrolysis rate in the presence of MYTA was 0.775 microM/min. LINEWEAVER and BURK plots showed that MYTA did not alter K(M) (1.31 mM) but decreased V(MAX). This study shows that MYTA exerts a non-competitive inhibition on the microsomal Na(+)/K(+) ATPase extracted from rabbit heart with a Ci(50) of 48 microg/ml. We conclude that the mechanism of action of MYTA is linked to the inhibition of the Na(+)/K(+) AT...
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