Background: Microorganisms and their by-products are responsible for establishing pulpal and peri... more Background: Microorganisms and their by-products are responsible for establishing pulpal and periapical diseases. Healing is compromised in patients under bisphosphonate therapy, and the presence of periapical infections can potentially lead to the development of medication-related osteonecrosis of the jaw (MRONJ). This work aimed to evaluate if bisphosphonate therapy is a risk factor for MRONJ development in the presence of periapical lesions. Methods: Two groups of 10 female Wistar rats were used. The experimental group received zoledronate (0.1 mg/kg) intraperitoneally, and the control received a saline solution, three times a week for three weeks. One week after the last injection, apical periodontitis was induced through pulpal exposure in the mandibular first molars. Twenty-one days later, the animals were intravenously injected with 99mTc-HMDP, and the radioactivity uptake by mandibular specimens was counted. In addition, sample radiographs and a histological examination were...
This study aimed to evaluate and compare the microleakage of composite resin restorations under a... more This study aimed to evaluate and compare the microleakage of composite resin restorations under a total-etch adhesive system applied with two different techniques, with and without cure. Cavities were made on the buccal surface of twenty-six intact teeth and subsequently restored with bulk-fill flow composite resin (SDR®® flow+) and conventional resin (Spectra™ ST HV). Two experimental groups were created, one where the total-etch adhesive (Prime & Bond Active®) was cured and another where the adhesive was not cured before placing the flowable resin. Two control groups were also created, negative and positive. After the restorations were finished the, teeth were submerged in a solution of sodium pertechnetate (99mTcNaO4) for 3 h to evaluate the microleakage. The results showed more infiltration of radioisotopes characterized by the highest total count values of microleakage in group 1 (cured adhesive) and in the positive control. Group 2 (noncured adhesive) and the negative control ...
Background: Obturation represents a critical step in endodontic treatment, which relies on a core... more Background: Obturation represents a critical step in endodontic treatment, which relies on a core material and a sealer. This study aims to evaluate the sealing ability and bond strength to the root canal walls of an epoxy resin-based sealer (AH-Plus®, Dentsply Sirona, Johnson City, TN, USA) and a bioceramic sealer (GuttaFlow Bioseal®, Coltène/Whaledent, GmbH + Co. KG, Langenau, Germany). Methods: Thirty-eight maxillary anterior teeth with single roots and identical round sections were separated into two experimental groups according to the root canal sealers used, namely, G1 = AH-Plus® and G2 = GuttaFlow Bioseal®, and two control groups, specifically, G3 = the negative control and G4 = the positive control. The sealing capacity was measured by the penetration of the radioactive isotope 99mTc. For the push-out test, the compressive force test was performed in a universal machine and the force was applied by exerting pressure on the surface of the material to be tested in the apical ...
Esthetic demands makes dental bleaching an increasingly requested therapy, however this is not a ... more Esthetic demands makes dental bleaching an increasingly requested therapy, however this is not a risk-free procedure due to the cytotoxicity of whitening products. This work evaluates possible cyto...
Background: Diffuse large B cell lymphoma (DLBCL) is recognized as a heterogeneous group of hemat... more Background: Diffuse large B cell lymphoma (DLBCL) is recognized as a heterogeneous group of hematological malignancies, and collectively forms the most common type of aggressive, non-Hodgkin lymphoma. The aim of our study was to evaluate the cellular and molecular effects of high doses X radiation in a DLBCL cell line. Materials and Methods: Farage cells were cultured and exposed to 0.5-60 Gy of Ionizing Radiation (IR). Cell viability and proliferation were assessed by trypan blue assay. Cell survival was determined with clonogenic assay. Cell death was assessed by flow cytometry (FC) and by optical microscopy. Cell cycle, mitochondrial membrane potential, reactive oxygen species, GSH and BAX/BCL-2 ratio were measured by FC. DNA damage was evaluated using comet assay. Total and phosphorylated P53 was assessed by western blot. Results: IR induced cytotoxic and cytostatic effects in Farage cells in a dose and time dependent manner with an LD50 of 1.73 Gy. Cell death occurs mainly by a...
AimTo investigate the association between root canal treatment outcome, diabetes mellitus, and al... more AimTo investigate the association between root canal treatment outcome, diabetes mellitus, and alterations of the angiogenic process.MethodologyA retrospective observational study was conducted in healthy (control group, CG) and diabetic (type II diabetes mellitus group, DG) patients after root canal treatment. The follow‐up appointments were performed to clinically and radiographically observe symptoms, the healing of periapical lesions and the quality of root fillings. In the animal model study, diabetic Goto‐Kakizaki (GK) rats and control Wistar rats were used. After 21 days of pulp exposure and the development of apical periodontitis (AP), the mandibles were removed for scintigraphic, radiographic, histopathological and molecular analyses. Chi‐square tests were performed to examine the variables related to endodontic outcome and differences between animal groups were assessed using the Student's t‐test.ResultsThe group of patients with diabetes had a significantly lower rate...
ObjectivesTo compare the treatments used to treat dentin hypersensitivity (DH), based on its effi... more ObjectivesTo compare the treatments used to treat dentin hypersensitivity (DH), based on its efficacy and effect duration.MethodsMedline/PubMed, Cochrane Library, EMBASE and ClinicalTrials were searched for articles published between 1 January 2008 and 14 November 2018, in English, Portuguese or Spanish, reporting clinical trials, completed and with results. This systematic review protocol was registered in PROSPERO, number CRD42019121986.ResultsSeventy‐four randomised clinical trials were included in the systematic review, reporting patients from 16 to 65 years old, with a clinical diagnosis of DH, that evaluate the efficacy of a desensitising product, compared to pre‐treatment, used the evaporative method stimulation and the visual analogue scale. These studies evaluated 5366 patients and at least 9167 teeth. Seven follow‐up periods were considered corresponding to an immediate, medium or long‐time effect. Sixty‐six studies were included in the quantitative synthesis. Glutaraldehy...
Cancer stem cells (CSCs) are a small population of resistant cells inhabiting the tumors. Althoug... more Cancer stem cells (CSCs) are a small population of resistant cells inhabiting the tumors. Although comprising only nearly 3% of the tumor mass, these cells were demonstrated to orchestrate tumorigenesis and differentiation, underlie tumors' heterogeneity and mediate therapy resistance and tumor relapse. Here we show that CSCs may be formed by dedifferentiation of terminally differentiated tumor cells under stress conditions. Using a elegant co-culture cellular system, we were able to prove that nutrients and oxygen deprivation activated non-malignant stromal fibroblasts, which in turn established with tumor cells a paracrine loop mediated by Interleukine-6 (IL-6), Activin-A and Granulocyte colony-stimulating factor (G-CSF), that drove subsequent tumor formation and cellular dedifferentiation. However, by scavenging these cytokines from the media and/or blocking exosomes' mediated communication it was possible to abrogate dedifferentiation thus turning these mechanisms into p...
The Journal of nutritional biochemistry, Jan 3, 2018
A diet rich in fiber is associated with a low risk of developing colorectal cancer. Dietary fiber... more A diet rich in fiber is associated with a low risk of developing colorectal cancer. Dietary fiber fermentation by intestinal microflora results in the production of butyrate, which has been reported as a chemopreventive agent and a histone deacetylase inhibitor (HDACi). Irinotecan is used as second-line treatment and induces adverse effects with serious life-threatening toxicities in at least 36% of patients. Our study intends to find a synergy that could improve the efficacy and decrease the toxicity of chemotherapy. Results demonstrate that milimolar concentrations of butyrate has an anti-proliferative effect in all three colon cancer cell lines under study, leading to a decrease on cell viability, expression of P21, P53 and β-catenin, being able to modulate P-glycoprotein activity and to induce apoptosis by modulation of BAX/BCL-2 ratio. Combined therapy has a cytotoxic potential, resulting in a synergistic effect, and allows a reduction in irinotecan concentration needed to redu...
Teduglutide is an enterotrophic analogue of glucagon-like peptide-2, with an indirect and poorly ... more Teduglutide is an enterotrophic analogue of glucagon-like peptide-2, with an indirect and poorly understood mechanism of action, approved for the rehabilitation of short-bowel syndrome. This study aims to analyze the response of tissue growth factors to surgical injury and teduglutide administration on an animal model of intestinal anastomosis. Wistar rats (n = 59) were distributed into four groups: "ileal resection" or "laparotomy", each one subdivided into "postoperative teduglutide administration" or "no treatment"; and sacrificed at the third or the seventh day, with ileal sample harvesting. Gene expression of insulin-like growth factor 1 (Igf1), vascular endothelial growth factor a (Vegfa), transforming growth factor β1 (Tgfβ1), connective tissue growth factor (Ctgf), fibroblast growth factor 2 (Fgf2), fibroblast growth factor 7 (Fgf7), epidermal growth factor (Egf), heparin-binding epidermal-like growth factor (Hbegf), platelet-derived g...
Background: Microorganisms and their by-products are responsible for establishing pulpal and peri... more Background: Microorganisms and their by-products are responsible for establishing pulpal and periapical diseases. Healing is compromised in patients under bisphosphonate therapy, and the presence of periapical infections can potentially lead to the development of medication-related osteonecrosis of the jaw (MRONJ). This work aimed to evaluate if bisphosphonate therapy is a risk factor for MRONJ development in the presence of periapical lesions. Methods: Two groups of 10 female Wistar rats were used. The experimental group received zoledronate (0.1 mg/kg) intraperitoneally, and the control received a saline solution, three times a week for three weeks. One week after the last injection, apical periodontitis was induced through pulpal exposure in the mandibular first molars. Twenty-one days later, the animals were intravenously injected with 99mTc-HMDP, and the radioactivity uptake by mandibular specimens was counted. In addition, sample radiographs and a histological examination were...
This study aimed to evaluate and compare the microleakage of composite resin restorations under a... more This study aimed to evaluate and compare the microleakage of composite resin restorations under a total-etch adhesive system applied with two different techniques, with and without cure. Cavities were made on the buccal surface of twenty-six intact teeth and subsequently restored with bulk-fill flow composite resin (SDR®® flow+) and conventional resin (Spectra™ ST HV). Two experimental groups were created, one where the total-etch adhesive (Prime & Bond Active®) was cured and another where the adhesive was not cured before placing the flowable resin. Two control groups were also created, negative and positive. After the restorations were finished the, teeth were submerged in a solution of sodium pertechnetate (99mTcNaO4) for 3 h to evaluate the microleakage. The results showed more infiltration of radioisotopes characterized by the highest total count values of microleakage in group 1 (cured adhesive) and in the positive control. Group 2 (noncured adhesive) and the negative control ...
Background: Obturation represents a critical step in endodontic treatment, which relies on a core... more Background: Obturation represents a critical step in endodontic treatment, which relies on a core material and a sealer. This study aims to evaluate the sealing ability and bond strength to the root canal walls of an epoxy resin-based sealer (AH-Plus®, Dentsply Sirona, Johnson City, TN, USA) and a bioceramic sealer (GuttaFlow Bioseal®, Coltène/Whaledent, GmbH + Co. KG, Langenau, Germany). Methods: Thirty-eight maxillary anterior teeth with single roots and identical round sections were separated into two experimental groups according to the root canal sealers used, namely, G1 = AH-Plus® and G2 = GuttaFlow Bioseal®, and two control groups, specifically, G3 = the negative control and G4 = the positive control. The sealing capacity was measured by the penetration of the radioactive isotope 99mTc. For the push-out test, the compressive force test was performed in a universal machine and the force was applied by exerting pressure on the surface of the material to be tested in the apical ...
Esthetic demands makes dental bleaching an increasingly requested therapy, however this is not a ... more Esthetic demands makes dental bleaching an increasingly requested therapy, however this is not a risk-free procedure due to the cytotoxicity of whitening products. This work evaluates possible cyto...
Background: Diffuse large B cell lymphoma (DLBCL) is recognized as a heterogeneous group of hemat... more Background: Diffuse large B cell lymphoma (DLBCL) is recognized as a heterogeneous group of hematological malignancies, and collectively forms the most common type of aggressive, non-Hodgkin lymphoma. The aim of our study was to evaluate the cellular and molecular effects of high doses X radiation in a DLBCL cell line. Materials and Methods: Farage cells were cultured and exposed to 0.5-60 Gy of Ionizing Radiation (IR). Cell viability and proliferation were assessed by trypan blue assay. Cell survival was determined with clonogenic assay. Cell death was assessed by flow cytometry (FC) and by optical microscopy. Cell cycle, mitochondrial membrane potential, reactive oxygen species, GSH and BAX/BCL-2 ratio were measured by FC. DNA damage was evaluated using comet assay. Total and phosphorylated P53 was assessed by western blot. Results: IR induced cytotoxic and cytostatic effects in Farage cells in a dose and time dependent manner with an LD50 of 1.73 Gy. Cell death occurs mainly by a...
AimTo investigate the association between root canal treatment outcome, diabetes mellitus, and al... more AimTo investigate the association between root canal treatment outcome, diabetes mellitus, and alterations of the angiogenic process.MethodologyA retrospective observational study was conducted in healthy (control group, CG) and diabetic (type II diabetes mellitus group, DG) patients after root canal treatment. The follow‐up appointments were performed to clinically and radiographically observe symptoms, the healing of periapical lesions and the quality of root fillings. In the animal model study, diabetic Goto‐Kakizaki (GK) rats and control Wistar rats were used. After 21 days of pulp exposure and the development of apical periodontitis (AP), the mandibles were removed for scintigraphic, radiographic, histopathological and molecular analyses. Chi‐square tests were performed to examine the variables related to endodontic outcome and differences between animal groups were assessed using the Student's t‐test.ResultsThe group of patients with diabetes had a significantly lower rate...
ObjectivesTo compare the treatments used to treat dentin hypersensitivity (DH), based on its effi... more ObjectivesTo compare the treatments used to treat dentin hypersensitivity (DH), based on its efficacy and effect duration.MethodsMedline/PubMed, Cochrane Library, EMBASE and ClinicalTrials were searched for articles published between 1 January 2008 and 14 November 2018, in English, Portuguese or Spanish, reporting clinical trials, completed and with results. This systematic review protocol was registered in PROSPERO, number CRD42019121986.ResultsSeventy‐four randomised clinical trials were included in the systematic review, reporting patients from 16 to 65 years old, with a clinical diagnosis of DH, that evaluate the efficacy of a desensitising product, compared to pre‐treatment, used the evaporative method stimulation and the visual analogue scale. These studies evaluated 5366 patients and at least 9167 teeth. Seven follow‐up periods were considered corresponding to an immediate, medium or long‐time effect. Sixty‐six studies were included in the quantitative synthesis. Glutaraldehy...
Cancer stem cells (CSCs) are a small population of resistant cells inhabiting the tumors. Althoug... more Cancer stem cells (CSCs) are a small population of resistant cells inhabiting the tumors. Although comprising only nearly 3% of the tumor mass, these cells were demonstrated to orchestrate tumorigenesis and differentiation, underlie tumors' heterogeneity and mediate therapy resistance and tumor relapse. Here we show that CSCs may be formed by dedifferentiation of terminally differentiated tumor cells under stress conditions. Using a elegant co-culture cellular system, we were able to prove that nutrients and oxygen deprivation activated non-malignant stromal fibroblasts, which in turn established with tumor cells a paracrine loop mediated by Interleukine-6 (IL-6), Activin-A and Granulocyte colony-stimulating factor (G-CSF), that drove subsequent tumor formation and cellular dedifferentiation. However, by scavenging these cytokines from the media and/or blocking exosomes' mediated communication it was possible to abrogate dedifferentiation thus turning these mechanisms into p...
The Journal of nutritional biochemistry, Jan 3, 2018
A diet rich in fiber is associated with a low risk of developing colorectal cancer. Dietary fiber... more A diet rich in fiber is associated with a low risk of developing colorectal cancer. Dietary fiber fermentation by intestinal microflora results in the production of butyrate, which has been reported as a chemopreventive agent and a histone deacetylase inhibitor (HDACi). Irinotecan is used as second-line treatment and induces adverse effects with serious life-threatening toxicities in at least 36% of patients. Our study intends to find a synergy that could improve the efficacy and decrease the toxicity of chemotherapy. Results demonstrate that milimolar concentrations of butyrate has an anti-proliferative effect in all three colon cancer cell lines under study, leading to a decrease on cell viability, expression of P21, P53 and β-catenin, being able to modulate P-glycoprotein activity and to induce apoptosis by modulation of BAX/BCL-2 ratio. Combined therapy has a cytotoxic potential, resulting in a synergistic effect, and allows a reduction in irinotecan concentration needed to redu...
Teduglutide is an enterotrophic analogue of glucagon-like peptide-2, with an indirect and poorly ... more Teduglutide is an enterotrophic analogue of glucagon-like peptide-2, with an indirect and poorly understood mechanism of action, approved for the rehabilitation of short-bowel syndrome. This study aims to analyze the response of tissue growth factors to surgical injury and teduglutide administration on an animal model of intestinal anastomosis. Wistar rats (n = 59) were distributed into four groups: "ileal resection" or "laparotomy", each one subdivided into "postoperative teduglutide administration" or "no treatment"; and sacrificed at the third or the seventh day, with ileal sample harvesting. Gene expression of insulin-like growth factor 1 (Igf1), vascular endothelial growth factor a (Vegfa), transforming growth factor β1 (Tgfβ1), connective tissue growth factor (Ctgf), fibroblast growth factor 2 (Fgf2), fibroblast growth factor 7 (Fgf7), epidermal growth factor (Egf), heparin-binding epidermal-like growth factor (Hbegf), platelet-derived g...
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Papers by Maria Filomena Botelho