Authors: Chalatsa, Ioanna | Arvanitis, Demetrios A. | Mikropoulou, Eleni V. | Giagini, Athina | Papadopoulou-Daifoti, Zeta | Aligiannis, Nektarios | Halabalaki, Maria | Tsarbopoulos, Anthony | Skaltsounis, Leandros A. | Sanoudou, Despina
Article Type:
Research Article
Abstract:
Background: Natural products are a significantly underutilized source of potential treatments against human disease. Alzheimer’s disease (AD) is a prime example of conditions that could be amenable to such treatments as suggested by recent findings. Objective: Aiming to identify novel potentially therapeutic approaches against AD, we assessed the effects of Cichorium spinosum and Sideritis scardica extracts, both distinct components of the Mediterranean diet. Methods/Results: After the detailed characterization of the extracts’ composition using LC-HRMS methods, they were evaluated on two AD neuronal cell culture models, namely the Aβ PP overexpressing SH-SY5Y-Aβ PP and the hyperphosphorylated tau expressing PC12-htau. Initially their
…effect on cell viability of SH-SY5Y and PC12 cells was examined, and subsequently their downstream effects on Aβ PP and tau processing pathways were investigated in the SH-SY5Y-Aβ PP and PC12-htau cells. We found that the S. scardica and C. spinosum extracts have similar effects on tau, as they both significantly decrease total tau, the activation of the GSK3β , ERK1 and/or ERK2 kinases of tau, as well as tau hyperphosphorylation. Furthermore, both extracts appear to promote Aβ PP processing through the alpha, non-amyloidogenic pathway, albeit through partly different mechanisms. Conclusions: These findings suggest that C. spinosum and S. scardica could have a notable potential in the prevention and/or treatment of AD, and merit further investigations at the in vivo level.
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Keywords: Alzheimer’s disease, amyloid, amyloidosis, Cichorium spinosum, Mediterranean diet, mountain tea, neurodegenerative diseases, prevention, Sideritis scardica, tauopathies
DOI: 10.3233/JAD-170862
Citation: Journal of Alzheimer's Disease,
vol. 64, no. 3, pp. 787-800, 2018
Price: EUR 27.50
