Authors: Zeng, Fu-Yue
Article Type: Research Article
Abstract: G protein-coupled receptors represent one of the largest families of cell surface receptors in nature. These receptors play fundamental roles in diverse physiological processes such as neurotransmission, cellular metabolism, cell differentiation and growth as well as immune response. In response to extracellular ligands, G protein-coupled receptors specifically interact with heterotrimeric G proteins that can then activate or inhibit effector enzymes, ultimately leading to the physiological response. Biochemical and mutational studies have revealed the molecular mechanisms of ligand-receptor interaction, receptor activation and receptor-G protein coupling selectivity. In the last few years, increasing evidence suggested that G protein-coupled receptor are also involved …in other novel signaling pathways in addition to classical G protein-mediated pathways. This review will briefly summarize recent studies on structural and functional aspects of G protein-coupled receptors with emphasis on molecular mechanisms of receptor activation. Show more
Keywords: G protein-coupled receptors, ligand-receptor interaction, receptor structure, receptor activation
Citation: Electronic Journal of Pathology and Histology, vol. 6, no. 1, pp. 13-13, 2000
Authors: Jiao, Yuwen | Fu, Yue | Gong, Yu | Wang, Guangyao | Chen, Shuai | Cai, Gengdi | Wu, Siyuan | Tang, Liming
Article Type: Research Article
Abstract: BACKGROUND: Gastric cancer (GC) remains a huge challenge to the heathy of human beings, largely due to lacking of effective therapeutic measures. Though an oncogenic role for circular RNAs (circRNAs) circ_0067997 in the progression of GC has been described recently, the molecular modulatory mechanism of it still remains to be further explored. The aim of present study is to examine the molecular network of circ_0067997 in GC. METHODS: qRT-PCR was carried out to determine the mRNA levels of circ_0067997, miR-615-5p and AKT1 in cisplatin (DDP)-insensitive or sensitive GC tumor tissues and cells, while the correlations among the contents of these …molecules were determined by statistical analysis. The expression of circ_0067997 was manipulated by short-hairpin RNA and lentiviral-mediated approaches, while that of miR-615-5p was achieved by the application of its inhibitor or mimic. The in vivo action of circ_0067997 on tumor formation was determined by measuring tumor weight/volume/size and analyzing tumor apoptosis through TUNEL staining in mouse xenograft model and, while the in vitro effects of this circRNA and its target miR-615-5p on the cell survival and death were separately evaluated by CCK-8 assay and flow cytometry. Additionally, luciferase reporter assays were executed to determine the sequentially regulatory relationships of circ_0067997, miR-615-5p, and AKT1. RESULTS: Our data demonstrated that the level of circ_0067997 level was increased in DDP-insensitive GC tissues and cell line, while miR-615-5p presented the opposite results. Moreover, the relationships between circ_0067997 and miR-615-5p levels, circ_0067997 and AKT1 contents presented negative and positive correlations in clinic samples, respectively. Importantly, circ_0067997 was found to repress miR-615-5p expression, consequently leading to increased growth while reduced apoptosis of GC cells in the presence of DDP. Furthermore, the validated sequential regulation was circ_0067997 modulating miR-615-5p adjusting AKT1. CONCLUSIONS: This study demonstrated that circ_0067997 functioned as a sponge of miR-615-5p to target AKT1 expression, thereby enhancing the growth and restricting the apoptosis of DDP-insensitive GC cells. These new findings offered a valuable target for the detection and management of GC. Show more
Keywords: Circ_0067997, Cell proliferation and apoptosis, gastric cancer, Cisplatin (DDP), miR-615-5p/AKT1 axis
DOI: 10.3233/CBM-220145
Citation: Cancer Biomarkers, vol. 37, no. 1, pp. 27-38, 2023
Screening and identification of key biomarkers in alimentary tract cancers: A bioinformatic analysis
Authors: Cai, Zeling | Wei, Yi | Chen, Shuai | Gong, Yu | Fu, Yue | Dai, Xianghua | Zhou, Yan | Yang, Haojun | Tang, Liming | Liu, Hanyang
Article Type: Research Article
Abstract: BACKGROUND: Alimentary tract cancers (ATCs) are the most malignant cancers in the world. Numerous studies have revealed the tumorigenesis, diagnosis and treatment of ATCs, but many mechanisms remain to be explored. METHODS: To identify the key genes of ATCs, microarray datasets of oesophageal cancer, gastric cancer and colorectal cancer were obtained from the Gene Expression Omnibus (GEO) database. In total, 207 differentially expressed genes (DEGs) were screened. KEGG and GO function enrichment analyses were conducted, and a protein-protein interaction (PPI) network was generated and gene modules analysis was performed using STRING and Cytoscape. RESULTS: Five hub genes were screened, and …the associated biological processes indicated that these genes were mainly enriched in cellular processes, protein binding and metabolic processes. Clinical survival analysis showed that COL10A1 and KIF14 may be significantly associated with the tumorigenesis or pathology grade of ATCs. In addition, relative human ATC cell lines along with blood samples and tumour tissues of ATC patients were obtained. The data proved that high expression of COL10A1 and KIF14 was associated with tumorigenesis and could be detected in blood. CONCLUSION: In conclusion, the identification of hub genes in the present study helped us to elucidate the molecular mechanisms of tumorigenesis and identify potential diagnostic indicators and targeted treatment for ATCs. Show more
Keywords: Alimentary tract cancers, differentially expressed genes, microarray datasets, enrichment analysis, survival analysis
DOI: 10.3233/CBM-201580
Citation: Cancer Biomarkers, vol. 29, no. 2, pp. 221-233, 2020