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Authors: Sun, Shu-Wei | Nishioka, Christopher | Labib, Wessam | Liang, Hsiao-Fang

Article Type: Research Article

Abstract: Background: Synaptic deficits and neuronal loss are the major pathological manifestations of Alzheimer’s disease. However, the link between the early synaptic loss and subsequent neurodegeneration is not entirely clear. Cell culture studies have shown that amyloid-β (Aβ) applied to axonal terminals can cause retrograde degeneration leading to the neuronal loss, but this process has not been demonstrated in live animals. Objective: To test if Aβ applied to retinal ganglion cell axonal terminals can induce axonal damage in the optic nerve and optic tract in mice. Methods: Aβ was injected into the terminal field of the optic tract, in the left …lateral geniculate nucleus of wildtype C57BL/6 mice. Following the injection, monthly diffusion tensor imaging was performed. Three months after the injection, mice underwent visual evoked potential recordings, and then sacrificed for immunohistochemical examination. Results: There were no significant changes seen with diffusion tensor imaging in the optic nerve and optic tract 3 months after the Aβ injection. The myelin and axons in these regions remained intact according to immunohistochemistry. The only significant changes observed in this study were delayed transduction and reduced amplitude of visual evoked potentials, although both Aβ and its reversed form caused similar changes. Conclusion: Despite the published in vitro studies, there was no significant axonal damage in the optic nerve and optic tract after injecting Aβ onto retinal ganglion cell axonal terminals of wildtype C57BL/6 mice. Show more

Keywords: Amyloid-β injection, diffusion tensor image, mouse, retinal ganglion cell, retrograde degeneration, visual evoked potential

DOI: 10.3233/JAD-142154

Citation: Journal of Alzheimer's Disease, vol. 45, no. 4, pp. 1139-1148, 2015

Authors: Sun, Shu-Wei | Liang, Hsiao-Fang | Mei, Jennifer | Xu, Dan | Shi, Wei-Xing

Article Type: Research Article

Abstract: Background: Diffusion tensor imaging (DTI) suggests the presence of white matter abnormality at the prodromal stage in human Alzheimer’s disease (AD). Objective: To use a mouse model of AD to determine whether the white matter abnormality detected by in vivo DTI is associated with functional deficits and axon damage. Methods: Amyloid-β1-42 (Aβ1-42 ) was injected into the left lateral ventricle in mice. Two months after the injection, in vivo DTI and visual evoked potential (VEP) recordings were performed, followed by immunohistochemistry of phosphorylated neurofilament and myelin basic protein. Results: DTI of Aβ1-42 -treated mice showed a significant increase of radial …diffusivity in white matter including the optic nerves and tracts. The abnormality was associated with decreased amplitude and increased latency of VEP. Immunohistochemistry confirmed a significant loss of axons and myelin integrity. Conclusion: White matter damage induced by Aβ1-42 in mice can be detected non-invasively by DTI. Show more

Keywords: Amyloid-β1-42, diffusion tensor imaging, mice, optic nerve, white matter diseases

DOI: 10.3233/JAD-130236

Citation: Journal of Alzheimer's Disease, vol. 38, no. 1, pp. 93-101, 2014