Abstract— Different amounts of dietary phospholipid were tested for their effect on the digestibi... more Abstract— Different amounts of dietary phospholipid were tested for their effect on the digestibility of total neutral lipids in the diet of the prawn Penaeus monodon. A purified source of the phospholipid, L-α-phosphatidylcholine, was included at 0. 5, 10, 20 or 40 g/kg in a purified diet containing 80 g/kg olive oil as the predominant neutral lipid source. The apparent digestibility of the neutral lipid (ADNL) in these diets was determined by comparing concentrations of the digestibility marker, cholestane in the feed and faeces of the prawns. ADNL (%) increased curvilinearly with increasing dietary phospholipid content (X, %) as described by the equation: ADNL = 80.4 + 9.08X -1.705X2 (P < 0.05; R2= 0.71); the asymptote of 92.5% was achieved with a phosphatidylcholine value of 27 g/kg. These results demonstrate that phosphatidylcholine significantly increases the digestibility of neutral lipids by penaeid prawns. However, olive oil was used as the predominant neutral lipid source to examine the effects with a relatively poorly digestible lipid source. The effects of phosphatidylcholine on more readily digested lipid sources such as fish oils, may not be as great, but still be sufficient to improve the utilisation of these nutrients.
The growth, condition, and feed utilization patterns of juvenile southern bluefin tuna (SBT) fed ... more The growth, condition, and feed utilization patterns of juvenile southern bluefin tuna (SBT) fed moist-pellets were examined over a 19-wk period from March to July 1999. The SBT had significant weight gain over the course of the study, increasing on average from about 27 to 34 kg (dependent on size class). No significant weight gain by the SBT occurred in the first 5 wk of the study. Following this initial period of slow growth, the rate of weight gain increased, ranging between 40 to 90 g/d. Weight gain peaked after 11 wk, with no fbrther gain occurring after this time point. Weight gain was strongly related to average daily feed intake (AFI) which was predominantly influenced by water temperature. Weight gain was minimal following the decrease of water temperatures below 15 C, consistent with a decrease in feed intake from peak values at the beginning of the study to basal levels by week 13. Basal feeding levels were maintained for the remainder of the study through to week 19. Although water temperature and AFI were strongly related, other time related effects also appeared to be signillcant. A relationship between condition index and feed intake was also identified. Condition index of the SBT increased from about 19 kg/m3 to 22 kg/m3 over the 19-wk period. Similar to the patterns observed in growth, there was also an initial delay in increases of condition index. The results from this study support that the majority of weight gain by juvenile SBT occurs during the early part of the production season and that this is most likely influenced indirectly through responses to water temperature. The results of this study also suggest that there is little value in conducting growth trials beyond the point where water temperatures decrease below 15 C. Although there was minimal weight gain or improvement in condition beyond the 11-wk time point, these parameters would need to be considered in conjunction with flesh quality characteristics to identify optimum harvesting regimes.
The evaluation of feed ingredients is crucial to nutritional research and feed development for aq... more The evaluation of feed ingredients is crucial to nutritional research and feed development for aquaculture species. In evaluating ingredients for use in aquaculture feeds, there are several important knowledge components that should be understood to enable the judicious use of a particular ingredient in feed formulation. This includes information on (1) ingredient digestibilities, (2) ingredient palatability and (3) nutrient utilization and interference.Diet design, feeding strategy, faecal collection method and method of calculation all have important implications on the determination of the digestible value of nutrients from any ingredient. There are several ways in which palatability of ingredients can be assessed, usually based on variable inclusion levels of the ingredient in question in a reference diet and feeding of those diets under an apparent satietal or self-regulating feeding regimes. However, the design of the diets, the parameters of assessment and the feeding regime can all be subject to variation depending on subtleties of the experimental design. Clearly, issues relating to feed intake are the key performance criteria in palatability assessments, and it is important that such experiments maintain sufficient stringency to allow some self-discrimination of the test feeds by the fish. The ability of fish to use nutrients from the test ingredient, or defining factors that interfere with that process, is perhaps the most complex and variable part of the ingredient evaluation process. It is crucial to discriminate effects on feed intake from effects on utilization of nutrients from ingredients (for growth and other metabolic processes). To allow an increased focus on nutrient utilization by the animals, there are several experimental strategies that can be adopted, which are based on variations in diet design and feeding regime used. Other issues such as ingredient functionality, influence on immune status and effects on organoleptic qualities are also important consideration in determining the value of ingredients in aquaculture feed formulations. A key aspect to note is the need to design all experiments with sufficient experimental capacity to detect significant effects.
Oxygen demand by all animals is driven primarily by their needs for sustaining metabolism. Typica... more Oxygen demand by all animals is driven primarily by their needs for sustaining metabolism. Typically, larger animals require more oxygen and cellular fuel to carry out respiration than smaller animals. This relationship in most cases is not linear and is usually described by a coefficient and exponent (e.g. axb): the exponent b showing the relationship between live-weight and energy/oxygen demand and is often termed the metabolic body weight (MBW) exponent, while the coefficient (a) tends to be temperature specific and describes the relationship between MBW and maintenance metabolic energy and oxygen demand at that specific temperature. Across all temperatures (range 26.0–32.0°C), the relationship between barramundi (Lates calcarifer) live-weight (x; g) and relative oxygen consumption as standard metabolic rate (y; mg O2 kg−1 h−1) at 29.4±1.5°C (mean±SD) was described by the exponential curve: y=710.19 x−0.3268, R2=0.6875 (n=222 assessments). Examination of the same data but on a gross oxygen consumption (mg O2 h−1) basis showed a relationship between live-weight (x; g) and gross oxygen consumption (y; mg O2 h−1) that was described by the exponential curve: y=0.710 x0.6732, R2=0.9033. Evaluation of the combined relationship between fish live-weight (y; g) and water temperature (x; °C) on gross oxygen consumption rate (z; mg O2 h−1) was described by the equation: z=(−20.7818+1.4017x−0.0227x2) ×y0.673.
... While some types of starch can be well digested ( [Bergot and Breque, 1983] , [Amirkolaie et ... more ... While some types of starch can be well digested ( [Bergot and Breque, 1983] , [Amirkolaie et al., 2006] , [Enes et al., 2008] and [Moreira et al., 2008] ), there ... Lupins are typically devoid of starch (&amp;amp;amp;lt; 10 g/kg), but have high levels of galactose based polysaccharides (Petterson, 2000 ...
Abstract— Different amounts of dietary phospholipid were tested for their effect on the digestibi... more Abstract— Different amounts of dietary phospholipid were tested for their effect on the digestibility of total neutral lipids in the diet of the prawn Penaeus monodon. A purified source of the phospholipid, L-α-phosphatidylcholine, was included at 0. 5, 10, 20 or 40 g/kg in a purified diet containing 80 g/kg olive oil as the predominant neutral lipid source. The apparent digestibility of the neutral lipid (ADNL) in these diets was determined by comparing concentrations of the digestibility marker, cholestane in the feed and faeces of the prawns. ADNL (%) increased curvilinearly with increasing dietary phospholipid content (X, %) as described by the equation: ADNL = 80.4 + 9.08X -1.705X2 (P < 0.05; R2= 0.71); the asymptote of 92.5% was achieved with a phosphatidylcholine value of 27 g/kg. These results demonstrate that phosphatidylcholine significantly increases the digestibility of neutral lipids by penaeid prawns. However, olive oil was used as the predominant neutral lipid source to examine the effects with a relatively poorly digestible lipid source. The effects of phosphatidylcholine on more readily digested lipid sources such as fish oils, may not be as great, but still be sufficient to improve the utilisation of these nutrients.
The growth, condition, and feed utilization patterns of juvenile southern bluefin tuna (SBT) fed ... more The growth, condition, and feed utilization patterns of juvenile southern bluefin tuna (SBT) fed moist-pellets were examined over a 19-wk period from March to July 1999. The SBT had significant weight gain over the course of the study, increasing on average from about 27 to 34 kg (dependent on size class). No significant weight gain by the SBT occurred in the first 5 wk of the study. Following this initial period of slow growth, the rate of weight gain increased, ranging between 40 to 90 g/d. Weight gain peaked after 11 wk, with no fbrther gain occurring after this time point. Weight gain was strongly related to average daily feed intake (AFI) which was predominantly influenced by water temperature. Weight gain was minimal following the decrease of water temperatures below 15 C, consistent with a decrease in feed intake from peak values at the beginning of the study to basal levels by week 13. Basal feeding levels were maintained for the remainder of the study through to week 19. Although water temperature and AFI were strongly related, other time related effects also appeared to be signillcant. A relationship between condition index and feed intake was also identified. Condition index of the SBT increased from about 19 kg/m3 to 22 kg/m3 over the 19-wk period. Similar to the patterns observed in growth, there was also an initial delay in increases of condition index. The results from this study support that the majority of weight gain by juvenile SBT occurs during the early part of the production season and that this is most likely influenced indirectly through responses to water temperature. The results of this study also suggest that there is little value in conducting growth trials beyond the point where water temperatures decrease below 15 C. Although there was minimal weight gain or improvement in condition beyond the 11-wk time point, these parameters would need to be considered in conjunction with flesh quality characteristics to identify optimum harvesting regimes.
The evaluation of feed ingredients is crucial to nutritional research and feed development for aq... more The evaluation of feed ingredients is crucial to nutritional research and feed development for aquaculture species. In evaluating ingredients for use in aquaculture feeds, there are several important knowledge components that should be understood to enable the judicious use of a particular ingredient in feed formulation. This includes information on (1) ingredient digestibilities, (2) ingredient palatability and (3) nutrient utilization and interference.Diet design, feeding strategy, faecal collection method and method of calculation all have important implications on the determination of the digestible value of nutrients from any ingredient. There are several ways in which palatability of ingredients can be assessed, usually based on variable inclusion levels of the ingredient in question in a reference diet and feeding of those diets under an apparent satietal or self-regulating feeding regimes. However, the design of the diets, the parameters of assessment and the feeding regime can all be subject to variation depending on subtleties of the experimental design. Clearly, issues relating to feed intake are the key performance criteria in palatability assessments, and it is important that such experiments maintain sufficient stringency to allow some self-discrimination of the test feeds by the fish. The ability of fish to use nutrients from the test ingredient, or defining factors that interfere with that process, is perhaps the most complex and variable part of the ingredient evaluation process. It is crucial to discriminate effects on feed intake from effects on utilization of nutrients from ingredients (for growth and other metabolic processes). To allow an increased focus on nutrient utilization by the animals, there are several experimental strategies that can be adopted, which are based on variations in diet design and feeding regime used. Other issues such as ingredient functionality, influence on immune status and effects on organoleptic qualities are also important consideration in determining the value of ingredients in aquaculture feed formulations. A key aspect to note is the need to design all experiments with sufficient experimental capacity to detect significant effects.
Oxygen demand by all animals is driven primarily by their needs for sustaining metabolism. Typica... more Oxygen demand by all animals is driven primarily by their needs for sustaining metabolism. Typically, larger animals require more oxygen and cellular fuel to carry out respiration than smaller animals. This relationship in most cases is not linear and is usually described by a coefficient and exponent (e.g. axb): the exponent b showing the relationship between live-weight and energy/oxygen demand and is often termed the metabolic body weight (MBW) exponent, while the coefficient (a) tends to be temperature specific and describes the relationship between MBW and maintenance metabolic energy and oxygen demand at that specific temperature. Across all temperatures (range 26.0–32.0°C), the relationship between barramundi (Lates calcarifer) live-weight (x; g) and relative oxygen consumption as standard metabolic rate (y; mg O2 kg−1 h−1) at 29.4±1.5°C (mean±SD) was described by the exponential curve: y=710.19 x−0.3268, R2=0.6875 (n=222 assessments). Examination of the same data but on a gross oxygen consumption (mg O2 h−1) basis showed a relationship between live-weight (x; g) and gross oxygen consumption (y; mg O2 h−1) that was described by the exponential curve: y=0.710 x0.6732, R2=0.9033. Evaluation of the combined relationship between fish live-weight (y; g) and water temperature (x; °C) on gross oxygen consumption rate (z; mg O2 h−1) was described by the equation: z=(−20.7818+1.4017x−0.0227x2) ×y0.673.
... While some types of starch can be well digested ( [Bergot and Breque, 1983] , [Amirkolaie et ... more ... While some types of starch can be well digested ( [Bergot and Breque, 1983] , [Amirkolaie et al., 2006] , [Enes et al., 2008] and [Moreira et al., 2008] ), there ... Lupins are typically devoid of starch (&amp;amp;amp;lt; 10 g/kg), but have high levels of galactose based polysaccharides (Petterson, 2000 ...
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