The transmission and prevention of gonococcal infection is a global health problem and an effecti... more The transmission and prevention of gonococcal infection is a global health problem and an effective drug or vaccine against the pathogen is yet to be developed. In our previous studies by analyzing metabolic pathways and membrane proteome of N. gonorrhoeae we found that four membrane associated targets could be better option in developing antigonorrhoeae drugs and vaccine. Here we showed that among these putative targets, D-alanine—D-alanine ligase (ddl) is the best candidate for development of both drug and ...
In spite of genome sequences of both human and N. gonorrhoeae in hand, vaccine for gonorrhea is y... more In spite of genome sequences of both human and N. gonorrhoeae in hand, vaccine for gonorrhea is yet not available. Due to availability of several host and pathogen genomes and numerous tools for in silico prediction of effective B-cell and T-cell epitopes; recent trend of vaccine designing has been shifted to peptide or epitope based vaccines that are more specific, safe, and easy to produce. In order to design and develop such a peptide vaccine against the pathogen, we adopted a novel computational approache based on sequence, ...
Integrative biology : quantitative biosciences from nano to macro, 2013
Although attempts have been made to unveil protein-protein and host-pathogen interactions based o... more Although attempts have been made to unveil protein-protein and host-pathogen interactions based on molecular insights of important biological events and pathogenesis in various organisms, these efforts have not yet been reported in Corynebacterium pseudotuberculosis (Cp), the causative agent of Caseous Lymphadenitis (CLA). In this study, we used computational approaches to develop common conserved intra-species protein-protein interaction (PPI) networks first time for four Cp strains (Cp FRC41, Cp 316, Cp 3/99-5, and Cp P54B96) followed by development of a common conserved inter-species bacterial PPI using conserved proteins in multiple pathogens (Y. pestis, M. tuberculosis, C. diphtheriae, C. ulcerans, E. coli, and all four Cp strains) and E. Coli based experimentally validated PPI data. Furthermore, the interacting proteins in the common conserved inter-species bacterial PPI were used to generate a conserved host-pathogen interaction (HP-PPI) network considering human, goat, sheep...
Gonococcal infection and transmission is a global health problem and till date no effective vacci... more Gonococcal infection and transmission is a global health problem and till date no effective vaccine is available to prevent the disease transmission. Recently, analysing membrane proteome of N. gonorrhoeae we have shown that, several membrane associated proteins of the gonococcus might be important in developing anti-gonorrhoea drugs. Here, we explored the possibility of such 19 essential membrane transporter targets those may be useful in developing peptide vaccines too. Using a classical in silico technique, we have ...
Although attempts have been made to unveil protein–protein and host–pathogen interactions based o... more Although attempts have been made to unveil protein–protein and host–pathogen interactions based on molecular insights of important biological events and pathogenesis in various organisms, these efforts have not yet been reported in Corynebacterium pseudotuberculosis (Cp), the causative agent of Caseous Lymphadenitis (CLA). In this study, we used computational approaches to develop common conserved intra-species protein–protein interaction (PPI) networks first time for four Cp strains (Cp FRC41, Cp 316, Cp 3/99-5, and Cp P54B96) followed by development of a common conserved inter-species bacterial PPI using conserved proteins in multiple pathogens (Y. pestis, M. tuberculosis, C. diphtheriae, C. ulcerans, E. coli, and all four Cp strains) and E. Coli based experimentally validated PPI data. Furthermore, the interacting proteins in the common conserved inter-species bacterial PPI were used to generate a conserved host–pathogen interaction (HP-PPI) network considering human, goat, sheep...
Abstract Seven-day-old kidney bean and cabbage seedlings were treated with 0.1–0.3 M NaCl solutio... more Abstract Seven-day-old kidney bean and cabbage seedlings were treated with 0.1–0.3 M NaCl solutions for 3 days. Chlorophyll content decreased in NaCl-treated Phaseolus seedlings, but did not significantly decrease in Brassica seedlings. Photochemical efficiency of photosystem II at dark-adapted state was similar in both Phaseolus and Brassica. The de-epoxidation state of violaxanthin increased more than sixfold in Phaseolus but showed no significant change in Brassica seedlings during NaCl treatment under low light. Maximum ...
Gonorrhea is one of the most common sexually transmitted diseases all over the world caused by Ne... more Gonorrhea is one of the most common sexually transmitted diseases all over the world caused by Neisseria gonorrhoeae. Till date only antibiotic treatment is recommended and no specific drug is available. The number of drug resistant stains is also increasing. In this study, using in silico subtractive genomics approach, membrane associated candidate drug targets have been identified in N. gonorrhoeae
Abstract: The genus Jatropha is distributed through out the tropics and sub-tropics growning in m... more Abstract: The genus Jatropha is distributed through out the tropics and sub-tropics growning in marginal lands and is a potential biodiesel crop world wide. The plants can prevent soil erosion, grown as a live fence and used as an alternate commercial crop. The seed oil can be used as a feed stock for biodiesel. Alternatively Jatropha oil is used in soap, glue or dye industry. The seed cake is rich in nitrogen and phosphorus, and can be used as manure. All parts of the plant including seeds have medicinal properties.
Phytohormones like IAA and kinetin inhibit chlorophyll loss during aging of wheat chloroplasts du... more Phytohormones like IAA and kinetin inhibit chlorophyll loss during aging of wheat chloroplasts duringin vivo andin vitro. GA, on the other hand, stimulates the pigment degradation during aging of attached leaves in contrast to its senescence inhibiting action in detached leaves and isolated chloroplasts. A shift in optimum concentration of hormone in inhibiting chlorophyll degradation suggests a differential pool size of endogenous hormone regulating aging of chloroplastsin vivo andin vitro. The retardation of chlorophyll loss by kinetin, IAA and GA during aging of chloroplastsin vitro would indicate that the effect of hormones in preventing yellowing of senescing leaves may be mediated through their direct action on chloroplasts.
The transmission and prevention of gonococcal infection is a global health problem and an effecti... more The transmission and prevention of gonococcal infection is a global health problem and an effective drug or vaccine against the pathogen is yet to be developed. In our previous studies by analyzing metabolic pathways and membrane proteome of N. gonorrhoeae we found that four membrane associated targets could be better option in developing antigonorrhoeae drugs and vaccine. Here we showed that among these putative targets, D-alanine—D-alanine ligase (ddl) is the best candidate for development of both drug and ...
In spite of genome sequences of both human and N. gonorrhoeae in hand, vaccine for gonorrhea is y... more In spite of genome sequences of both human and N. gonorrhoeae in hand, vaccine for gonorrhea is yet not available. Due to availability of several host and pathogen genomes and numerous tools for in silico prediction of effective B-cell and T-cell epitopes; recent trend of vaccine designing has been shifted to peptide or epitope based vaccines that are more specific, safe, and easy to produce. In order to design and develop such a peptide vaccine against the pathogen, we adopted a novel computational approache based on sequence, ...
Integrative biology : quantitative biosciences from nano to macro, 2013
Although attempts have been made to unveil protein-protein and host-pathogen interactions based o... more Although attempts have been made to unveil protein-protein and host-pathogen interactions based on molecular insights of important biological events and pathogenesis in various organisms, these efforts have not yet been reported in Corynebacterium pseudotuberculosis (Cp), the causative agent of Caseous Lymphadenitis (CLA). In this study, we used computational approaches to develop common conserved intra-species protein-protein interaction (PPI) networks first time for four Cp strains (Cp FRC41, Cp 316, Cp 3/99-5, and Cp P54B96) followed by development of a common conserved inter-species bacterial PPI using conserved proteins in multiple pathogens (Y. pestis, M. tuberculosis, C. diphtheriae, C. ulcerans, E. coli, and all four Cp strains) and E. Coli based experimentally validated PPI data. Furthermore, the interacting proteins in the common conserved inter-species bacterial PPI were used to generate a conserved host-pathogen interaction (HP-PPI) network considering human, goat, sheep...
Gonococcal infection and transmission is a global health problem and till date no effective vacci... more Gonococcal infection and transmission is a global health problem and till date no effective vaccine is available to prevent the disease transmission. Recently, analysing membrane proteome of N. gonorrhoeae we have shown that, several membrane associated proteins of the gonococcus might be important in developing anti-gonorrhoea drugs. Here, we explored the possibility of such 19 essential membrane transporter targets those may be useful in developing peptide vaccines too. Using a classical in silico technique, we have ...
Although attempts have been made to unveil protein–protein and host–pathogen interactions based o... more Although attempts have been made to unveil protein–protein and host–pathogen interactions based on molecular insights of important biological events and pathogenesis in various organisms, these efforts have not yet been reported in Corynebacterium pseudotuberculosis (Cp), the causative agent of Caseous Lymphadenitis (CLA). In this study, we used computational approaches to develop common conserved intra-species protein–protein interaction (PPI) networks first time for four Cp strains (Cp FRC41, Cp 316, Cp 3/99-5, and Cp P54B96) followed by development of a common conserved inter-species bacterial PPI using conserved proteins in multiple pathogens (Y. pestis, M. tuberculosis, C. diphtheriae, C. ulcerans, E. coli, and all four Cp strains) and E. Coli based experimentally validated PPI data. Furthermore, the interacting proteins in the common conserved inter-species bacterial PPI were used to generate a conserved host–pathogen interaction (HP-PPI) network considering human, goat, sheep...
Abstract Seven-day-old kidney bean and cabbage seedlings were treated with 0.1–0.3 M NaCl solutio... more Abstract Seven-day-old kidney bean and cabbage seedlings were treated with 0.1–0.3 M NaCl solutions for 3 days. Chlorophyll content decreased in NaCl-treated Phaseolus seedlings, but did not significantly decrease in Brassica seedlings. Photochemical efficiency of photosystem II at dark-adapted state was similar in both Phaseolus and Brassica. The de-epoxidation state of violaxanthin increased more than sixfold in Phaseolus but showed no significant change in Brassica seedlings during NaCl treatment under low light. Maximum ...
Gonorrhea is one of the most common sexually transmitted diseases all over the world caused by Ne... more Gonorrhea is one of the most common sexually transmitted diseases all over the world caused by Neisseria gonorrhoeae. Till date only antibiotic treatment is recommended and no specific drug is available. The number of drug resistant stains is also increasing. In this study, using in silico subtractive genomics approach, membrane associated candidate drug targets have been identified in N. gonorrhoeae
Abstract: The genus Jatropha is distributed through out the tropics and sub-tropics growning in m... more Abstract: The genus Jatropha is distributed through out the tropics and sub-tropics growning in marginal lands and is a potential biodiesel crop world wide. The plants can prevent soil erosion, grown as a live fence and used as an alternate commercial crop. The seed oil can be used as a feed stock for biodiesel. Alternatively Jatropha oil is used in soap, glue or dye industry. The seed cake is rich in nitrogen and phosphorus, and can be used as manure. All parts of the plant including seeds have medicinal properties.
Phytohormones like IAA and kinetin inhibit chlorophyll loss during aging of wheat chloroplasts du... more Phytohormones like IAA and kinetin inhibit chlorophyll loss during aging of wheat chloroplasts duringin vivo andin vitro. GA, on the other hand, stimulates the pigment degradation during aging of attached leaves in contrast to its senescence inhibiting action in detached leaves and isolated chloroplasts. A shift in optimum concentration of hormone in inhibiting chlorophyll degradation suggests a differential pool size of endogenous hormone regulating aging of chloroplastsin vivo andin vitro. The retardation of chlorophyll loss by kinetin, IAA and GA during aging of chloroplastsin vitro would indicate that the effect of hormones in preventing yellowing of senescing leaves may be mediated through their direct action on chloroplasts.
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