IL-18 expression in clinical human pituitary adenoma
Abstract
Background:
IL-18 is known as an interferon-inducing factor that belongs to the IL-1 family, and is synthesized as an inactive precursor protein.
Objective:
The present study aims to investigate the expression of IL-18, IL-18R, R and IL-18 binding protein (BP) mRNA in various types of human pituitary tumors, such as adrenocorticotropic hormone (ACTH), growth hormone (GH), prolactin (PRL), thyroid stimulating hormone (TSH)-producing adenomas and non-function adenomas.
Methods:
Pituitary adenoma tissues were obtained during the surgery of 41 patients: nine patients had ACTH-producing pituitary adenomas, nine patients had GH-producing pituitary adenomas, five patients had TSH-producing pituitary adenomas, seven patients had PRL-producing pituitary adenomas, and 11 patients had non-functioning adenomas. The mRNA expression levels of IL-18, IL-18BP, IL-18R and IL-18R were quantified using real-time quantitative PCR.
Results:
The mRNA expression of IL-18 was significantly higher in ACTH-, GH- and PRL-producing adenomas, when compared to non-function tumors. Similarly, a significantly higher mRNA expression of IL-18BP and IL-18R was observed in ACTH-, GH- and PRL-producing adenomas, when compared with non-functional adenomas. In contrast, no upregulation of IL-18R mRNA was observed in any of the pituitary adenomas.
Conclusions:
The mRNA levels of IL-18, IL-18BP and IL-18R are significantly elevated in clinical pituitary tumors, such as ACTH-, GH- and PRL-producing adenomas, when compared to non-functional adenomas. These present results suggest the possibility that IL-18 may be involved in the pathogenesis of pituitary adenoma.
References
[1]
Nakanishi K. Unique Action of Interleukin-18 on T Cells and Other Immune Cells. Front Immunol. 2018; 9: 763.
[2]
Sharma A, Steven S, Bosmann M. The pituitary gland prevents shock-associated death by controlling multiple inflammatory mediators. Biochem Biophys Res Commun. 2019; 509(1): 188-193.
[3]
Nagai Y, Nochi T, Watanabe K, Watanabe K, Aso H, Kitazawa H, et al. Localization of interleukin-18 and its receptor in somatotrophs of the bovine anterior pituitary gland. Cell Tissue Res. 2005; 322(3): 455-62.
[4]
Majima T, Ichikura T, Chochi K, Kawabata T, Tsujimoto H, Sugasawa H, et al. Exploitation of interleukin-18 by gastric cancers for their growth and evasion of host immunity. Int J Cancer. 2006; 118(2): 388-95.
[5]
Park H, Byun D, Kim TS, Kim YI, Kang JS, Hahm ES, et al. Enhanced IL-18 expression in common skin tumors. Immunol Lett. 2001; 79(3): 215-9.
[6]
Eissa SA, Zaki SA, El-Maghraby SM, Kadry DY. Importance of serum IL-18 and RANTES as markers for breast carcinoma progression. J Egypt Natl Canc Inst. 2005; 17(1): 51-5.
[7]
Srabović N, Mujagić Z, Mujanović-Mustedanagić J, Muminović Z, Cickusić E. Interleukin 18 expression in the primary breast cancer tumour tissue. Med Glas (Zenica). 2011; 8(1): 109-15.
[8]
Chang CM, Wang ML, Lu KH, Yang YP, Juang CM, Wang PH, et al. Integrating the dysregulated inflammasome-based molecular functionome in the malignant transformation of endometriosis-associated ovarian carcinoma. Oncotarget. 2017; 9(3): 3704-3726.
[9]
Amo Y, Ohta Y, Hamada Y, Katsuoka K. Serum levels of interleukin-18 are increased in patients with cutaneous T-cell lymphoma and cutaneous natural killer-cell lymphoma. Br J Dermatol. 2001; 145(4): 674-6.
[10]
Tangkijvanich P, Thong-Ngam D, Mahachai V, Theamboonlers A, Poovorawan Y. Role of serum interleukin-18 as a prognostic factor in patients with hepatocellular carcinoma. World J Gastroenterol. 2007; 13(32): 4345-9.
[11]
Street SE, Trapani JA, MacGregor D, Smyth MJ. Suppression of lymphoma and epithelial malignancies effected by interferon gamma. J Exp Med. 2002; 196(1): 129-34.
[12]
Ahechu P, Zozaya G, Martí P, Hernández-Lizoáin JL, Baixauli J, Unamuno X, et al. NLRP3 Inflammasome: A Possible Link Between Obesity-Associated Low-Grade Chronic Inflammation and Colorectal Cancer Development. Front Immunol. 2018; 9: 2918.
[13]
Pagès F, Berger A, Henglein B, Piqueras B, Danel C, Zinzindohoue F, et al. Modulation of interleukin-18 expression in human colon carcinoma: consequences for tumor immune surveillance. Int J Cancer. 1999; 84(3): 326-30.
[14]
Cao R, Farnebo J, Kurimoto M, Cao Y. Interleukin-18 acts as an angiogenesis and tumor suppressor. FASEB J. 1999; 13(15): 2195-202.
[15]
Kaplanski G. Interleukin-18: Biological properties and role in disease pathogenesis. Immunol Rev. 2018; 281: 138-153.
[16]
Zhang H, Wang J, Wang L, Xie H, Chen L, He S. Role of IL-18 in atopic asthma is determined by balance of IL-18/IL-18BP/IL-18R. J Cell Mol Med. 2018; 22(1): 354-373.
[17]
Chandrasekar B, Colston JT, de la Rosa SD, Rao PP, Freeman GL. TNF-alpha and H2O2 induce IL-18 and IL-18R beta expression in cardiomyocytes via NF-kappa B activation. Biochem Biophys Res Commun. 2003; 303(4): 1152-8.
[18]
Hurgin V, Novick D, Rubinstein M. The promoter of IL-18 binding protein: activation by an IFN-gamma -induced complex of IFN regulatory factor 1 and CCAAT/enhancer binding protein beta. Proc Natl Acad Sci U S A. 2002; 99(26): 16957-62.
[19]
Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact. 2006; 160(1): 1-40.
[20]
Fontella FU, Siqueira IR, Vasconcellos AP, Tabajara AS, Netto CA, Dalmaz C. Repeated restraint stress induces oxidative damage in rat hippocampus. Neurochem Res. 2005; 30(1): 105-11.
[21]
Kim ST, Choi JH, Chang JW, Kim SW, Hwang O. Immobilization stress causes increases in tetrahydrobiopterin, dopamine, and neuromelanin and oxidative damage in the nigrostriatal system. J Neurochem. 2005; 95(1): 89-98.
Index Terms
- IL-18 expression in clinical human pituitary adenoma
Index terms have been assigned to the content through auto-classification.
Recommendations
Prognostic Factor Analysis for Breast Cancer Using Gene Expression Profiles
DTMBIO '15: Proceedings of the ACM Ninth International Workshop on Data and Text Mining in Biomedical InformaticsThe survival of patients with breast cancer is highly sporadic, from a few months to more than 15 years. Recently, the large-scale gene expression profiling of tumors has been used as a promising means of predicting prognosis factors. In this study, we ...
Microarray analysis reveals CC chemokine CCL-1 responsive gene expression in human HeLa cells
SCSC '07: Proceedings of the 2007 Summer Computer Simulation ConferenceHuman CC chemokine, CCL-1 is a chemotactic cytokine implicated in a variety of biological responses, namely inflammation and chemotaxis, in many cell types. We have previously shown that the CC chemokine, CCL-1 and its receptor CCR8 are expressed and ...
MicroRNA Expression Profiling of Pancreatic Cancer Histological Subtypes in the Identification of Potential Biomarkers for Diagnosis and Tumor Prognosis
ICBRA '23: Proceedings of the 2023 10th International Conference on Bioinformatics Research and ApplicationsPancreatic cancer (PC) is the seventh leading cause of cancer-related deaths and 12th in malignant frequency globally. It has a significantly high incidence rate in countries with high human development index (HDI) and, generally, regions able to use ...
Comments
Information & Contributors
Information
Published In
© 2022 – IOS Press. All rights reserved.
Publisher
IOS Press
Netherlands
Publication History
Published: 01 January 2022
Author Tags
Qualifiers
- Research-article
Contributors
Other Metrics
Bibliometrics & Citations
Bibliometrics
Article Metrics
- 0Total Citations
- 0Total Downloads
- Downloads (Last 12 months)0
- Downloads (Last 6 weeks)0
Reflects downloads up to 28 Jan 2025