Abstract
An endophytic bacteriumn identified as Acinetobacter johnsonii strain 3–1 was isolated from surface-sterilized roots of Beta vulgaris. Its effect on sugar beet seedling growth was studied using pot assays and field experiments. This strain promoted beet seedling growth following seed inoculation by seed dipping. Plant height and dry weight of beet increased by 19% and 69%, respectively, compared with controls. Strain 3–1 exhibited the ability to increase absorption of N, P, K, and Mg elements from soil and increase the content of vitamins B and C, and protein within beet. In addition, the strain also produced a phytohormone-auxin, produced nearly twice as much IAA as that produced by strain 2–2, and was able to solubilize phosphates. The concentration of dissolved P in the medium was 180.5 mg L−1 after 4 days of incubation. In field experiments, strain 3–1 significantly increased the content of sucrose, fructose, and the yield of the beet. The growth-promoting properties of Acinetobacter johnsonii strain 3–1 indicates that this promising isolate merits further investigation into its symbiosis with beet plants and its potential application in agriculture.
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References
Ali B, Sabri AN, Ljung K, Hasnain S (2009) Auxin production by plant associated bacteria: impact on endogenous IAA content and growth of Triticum aestivum L. Lett Appl Microbiol 48:542–547
Antoun H, Prevost D (2006) Ecology of plant growth promoting rhizobacteria. In: Siddiqui ZA (ed) PGPR: Biocontrol and Biofertilization. Springer, Dordrecht, pp 1–38
Basile DV, Basile MR, Li QY, Corpe WA (1985) Vitamin B12- stimulated growth and development of Jungermannia leiantha Grolle and Gymnocolea inflata (Huds.) Dum. (Hepaticae). Bryologist 88:77–81
Brooks DS, Gonzalez CF, Appel DN, Filer TH (1994) Evaluation of endophytic bacteria as potential biological control agents for oak wilt. Biol Control 4:373–381
Caitriona D, Wilson LW, McFadden H (2004) Gene expression profile changes in cotton root and hypocotyl tissues in response to infection with Fusarium oxysporum f. sp. Vasinfectum Mol Plant Microbe In 17:654–667
Chabot R, Antoun H, Cescas MP (1996) Growth promotion of maize and lettuce by phosphate solubilizing Rhizobium leguminosarium biovar phaseoli. Plant Soil 184:311–321
Compant S, Duffy B, Nowak J, Clement C, Barka EA (2005a) Use of plant growth-promoting bacteria for biocontrol of plant diseases: principles, mechanisms of action, and future prospects. Appl Environ Microbiol 71:4951–4959
Compant S, Reiter B, Sessitsch A, Nowak J, Clement C, Barka EA (2005b) Endophytic colonization of Vitis vinifera L. by a plant growth-promoting bacterium, Burkholderia sp. Strain PsJN. Appl Environ Microbiol 71:1685–1693
Dworkin M, Foster J (1958) Experiments with some microorganisms which utilize ethane and hydrogen. J Bacteriol 75:592–601
El-Shanshoury AR, El-Sououd SMA, Awadalla OA, El-Bandy NB (1996) Effects of Streptomyces corchorusii, Streptomyces mutabilis, pendimethalin and metribuzin on the control of bacterial and Fusarium wilt of tomato. Can J Bot 74:1016–1022
Fisher PJ, Petrini O, Lazpin SHM (1992) The distribution of some fungal and bacterial endophytes in maize (Zea mays L.). New Phytol 122:299–305
Glick BR, Karaturovic DM, Newell PC (1995) A novel procedure for rapid isolation of plant growth promoting pseudomonas. Can J Microbiol 41:533–536
Gordon SA, Weber RP (1951) Colorimetric estimation of indoleacetic acid. J Plant Physiol 26:192–195
He H, Qiu SX, Cai XQ, Guan X, Hu FP (2004) Colonization in plants and identification of endophytic bacteria BS-1 and BS-2 from Capsicum annuum. Acta Microbiol Sinica 44:13–18
Holland MA (1997) Occams razor applied to hormonology (Are cytokinins produced by plants?). J Plant Physiol 115:865–868
Holland MA, Polacco JC (1992) Urease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants. J Plant Physiol 98:942–948
Holland MA, Polacco JC (1994) PPFMs and other covert contamination: is there more to plant physiology than just plant? Ann Rev Plant Physiol Plant Mol Biol 45:197–209
Jacobs MJ, William MB, David AG (1985) Enumeration, location, characterization of endophytic bacteria within sugar beet roots. Can J Bot 63:1262–1265
Kennedy IR, Choudhury ATMA, Kecskes ML (2004) Nonsymbiotic bacterial diazotrophs in crop-farming systems: can their potential for plant growth promotion be better exploited? Soil Biol Biochem 36:1229–1244
Kodaka H, Mizuochi S, Teramura H, Nirazuka T (2005) Comparison of the compact dry TC method with the standard pour plate method (AOAC official method 966.23) for determining aerobic colony counts in food samples: Performance-tested method. J AOAC Int 88:1702–2713
Larran S, Monaco C, Alippi HE (2004) Endophytic fungi in beet (Beta vulgaris var. esculenta L.) leaves. Adv Hortic Sci 4:193–196
Yao T (2004) Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region II Phosphate-solubilizing power and auxin production. Acta Pratacultural Science 3:85–90
Liu SF, Tang WH (1996) The study on endophytic streptomyces of cotton. In: Tang WH, Cook RJ, Rovira A (eds) Advances in biological control of plant diseases. China Agricultural University Press, China, pp 212–213
Lucas-Garca JA, Schloter M, Durkaya T, Hartmann A, Gutirrez-Maero FJ (2003) Colonization of pepper roots by a plant growth promoting Pseudomonas fluorescens strain. Biol Fertil Soils 37:381–385
Mayak S, Tirosh T, Glick BR (2004) Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem 42:565–572
Patten CL, Glick BR (2002) Role of Pseudomonas putida indoleacetic acid in development of the host plant root system. Appl Environ Microbiol 68:3795–3801
Pieterse CMJ, Van Wees SCM, Ton J, Van Pelt JA, Van Loon LC (2002) Signalling in rhizobacteria-induced systemic resistance in Arabidopsis thaliana. Plant Biol 5:535–544
Rajkumar M, Nagendran R, Lee KJ, Lee WH, Kim SZ (2006) Influence of plant growth promoting bacteria and Cr6+ on the growth of Indian mustard. Chemosphere 62:741–748
Riehm H (1985) Arbeitsvorschrift zur Bestimmung der Phosphorsaure und des Kaliums nach der Laktatmethode. Zeitschrift fur Pflanzen Dungung und Bodenkunde 40:152–156
Schachtschnabel P, Heinemann CG (1974) Beziehungen zwischen den Kaliumgehalten in Boden und in jungen Haferpflanzen. Zeitschrift fur Pflanzen Dungung und Bodenkunde 137:123–134
Taylor KACC (1995) A colorimetric method for the quantitation of fructose. Appl Biochem Biotechnol 53:215–227
Zhang XS (2008) Analysis of the factors affecting the available P content in the fermentation liquid of P bacteria determined by Mo-Sb colorimetry. Journal of Anhui Agri Sci 36:4822–4823
Zhao XR, Lin QM, Li BG (2003) The relationship between rock phosphate solubilization and pH and organic acid production of microorganisms. J Microbiol 23:5–7
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This work was supported by the National Natural Science Foundation of China (Project No. 31060018).
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Shi, Y., Lou, K. & Li, C. Growth promotion effects of the endophyte Acinetobacter johnsonii strain 3-1 on sugar beet. Symbiosis 54, 159–166 (2011). https://doi.org/10.1007/s13199-011-0139-x
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DOI: https://doi.org/10.1007/s13199-011-0139-x