Abstract
General principles are formulated for the objective reconstruction of a three dimensional object from a set of electron microscope images. These principles are applied to the calculation of a three dimensional density map of the tail of bacteriophage T4.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on SpringerLink
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Klug, A., and Berger, J. E., J. Mol. Biol., 10, 565 (1964).
Klug, A., and De Rosier, D. J., Nature, 212, 29 (1966).
Klug, A., Crick, F. H. C., and Wyckoff, H. W., Acta Cryst., 11, 199 (1958).
Leith, E. N., and Upatnieks, J., J. Opt. Soc. Amer., 54, 1295 (1964).
Stroke, G. W., App. Phys. Lett., 6, 201 (1965).
Bragg, W. L., Nature, 166, 399 (1950).
Vander Lugt, A., Trans. IREE, IT-10, 139 (1964).
Brenner, S., Streisinger, G., Horne, R. W., Champe, S. P., Barnett, L., Benzer, S., and Rees, M. W., J. Mol. Biol., 1, 281 (1959).
Finch, J. T., Klug, A., and Nermut, M. V., J. Cell Sci., 2, (December, 1967).
Moody, M. F., J. Mol. Biol., 25, 201 (1967).
Krimm, S., and Anderson, T. F., J. Mol. Biol., 27, 197 (1967).
See for example, Kellenberger, E., and Boy de la Tour, E., J. Ultrastruct. Res., 11, 545 (1964).
Williams, R. C., and Fraser, D., Virology, 2, 289 (1956).
Hall, C. E., Introduction to Electron Microscopy ch. 9 (McGraw-Hill, New York, 1953).
Heidenreich, R. D., Fundamentals of Transmission Electron Microscopy, ch. 1 (Interscience, London, 1964).
Various contributions in Symp. on Quantitative Electron Microscopy (edit. by Bahr, G. F., and Zeitler, E. H.) (The Williams and Wilkins Company, Baltimore, 1965).
Franklin, R. E., and Klug, A., Acta Cryst., 8, 777 (1955).
Finch, J. T., and Klug, A., J. Mol. Biol., 15, 344 (1966).
Klug, A., and Finch, J. T., J. Mol. Biol. (in the press, 1968).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DE ROSIER, D., KLUG, A. Reconstruction of Three Dimensional Structures from Electron Micrographs. Nature 217, 130â134 (1968). https://doi.org/10.1038/217130a0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1038/217130a0
This article is cited by
-
Real-time 3D analysis during electron tomography using tomviz
Nature Communications (2022)
-
Observation of formation and local structures of metal-organic layers via complementary electron microscopy techniques
Nature Communications (2022)
-
Grayscale uncertainty and errors of tomographic reconstructions based on projection geometries and projection sets
The Visual Computer (2022)
