Location via proxy:   [ UP ]  
[Report a bug]   [Manage cookies]                
Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Identification of an enzyme in platelet microsomes which generates thromboxane A2 from prostaglandin endoperoxides

Nature volume 261, pages 558–560 (1976)Cite this article

Abstract

The microsomal fraction of horse and human platelets contains an enzyme which converts prostaglandin cyclic endoperoxides (PGG2 or PGH2) to a substance which is much more potent in contracting strips of rabbit aorta. This substance has the same characteristics as thromboxane A2, and can be distinguished from other products of arachidonic acid metabolism by differential bioassay.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on SpringerLink
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Piper, P. J., and Vane, J. R., Nature, 223, 29–35 (1969).

    Article  ADS  CAS  Google Scholar 

  2. Piper, P. J., and Vane, J. R., Ann. N. Y. Acad. Sci., 180, 363–385 (1971).

    Article  ADS  CAS  Google Scholar 

  3. Vargaftig, B. B., and Dao, N., Pharmacology, 6, 99–108 (1971).

    Article  CAS  Google Scholar 

  4. Palmer, M. A., Piper, P. J., and Vane, J. R., Br. J. Pharmac., 49, 226–242 (1973).

    Article  CAS  Google Scholar 

  5. Gryglewski, R., and Vane, J. R., Br. J. Pharmac., 46, 449–457 (1972).

    Article  CAS  Google Scholar 

  6. Willis, A. L., Circulation, 48, Suppl. 4, 55 (1973).

    Google Scholar 

  7. Willis, A. L., Science, 183, 325–327 (1974).

    Article  ADS  CAS  Google Scholar 

  8. Vargaftig, B. B., and Zirinis, P., Nature new Biol., 244, 114–116 (1973).

    Article  CAS  Google Scholar 

  9. Vargaftig, B. B., Tranier, Y., and Chignard, M., Prostaglandins, 8, 133–156 (1974).

    Google Scholar 

  10. Svensson, J., Hamberg, M., and Samuelsson, B., Acta physiol. scand., 94, 222–228 (1975).

    Article  CAS  Google Scholar 

  11. Nugteren, D. H., Beerthuis, R. K., and van Dorp, D. A., Proc. Second Nobel Symp. (edit. by Bergstrom, S., and Samuelsson, B.), 45–50 (Nobel Foundation, Stockholm, 1967).

    Google Scholar 

  12. Samuelsson, B., Granstrom, E., and Hamberg, M., Proc. Second Nobel Symp. (edit. Bergstrom, S., and Samulesson, B.), 31–44 (Nobel Foundation, Stockholm, 1967).

    Google Scholar 

  13. Hamberg, M., and Samuelsson, B., Proc. natn. Acad. Sci. U.S.A., 70, 899–903 (1973).

    Article  ADS  CAS  Google Scholar 

  14. Nugteren, D. H., and Hazelhof, E., Biochim. biophys. Acta, 326, 448–461 (1973).

    Article  CAS  Google Scholar 

  15. Hamberg, M., Hedqvist, P., Strandberg, K., Svensson, J., and Samuelsson, B., Life Sci., 16, 451–462 (1975).

    Article  CAS  Google Scholar 

  16. Hamberg, M., Svensson, J., Wakabayashi, T., and Samuelsson, B., Proc. natn. Acad. Sci. U.S.A., 71, 345–349 (1974).

    Article  ADS  CAS  Google Scholar 

  17. Smith, J. B., Ingerman, C., Kocsis, J. J., and Silver, M. J., J. clin. Invest., 53, 1468–1472 (1974).

    Article  CAS  Google Scholar 

  18. Zucker, M. B., and Peterson, J., Proc. Soc. exp. Biol. Med., 127, 547–551 (1968).

    Article  CAS  Google Scholar 

  19. Hamberg, M., Svensson, J., and Samuelsson, B., Proc. natn. Acad. Sci. U.S.A., 72, 2994–2998 (1975).

    Article  ADS  CAS  Google Scholar 

  20. Gornal, A. G., Bardawill, C. J., and David, M. M., J. biol. Chem., 177, 751–776 (1949).

    Google Scholar 

  21. Vane, J. R., Br. J. Pharmac., 12, 344–349 (1957).

    CAS  Google Scholar 

  22. Gilmore, N., Vane, J. R., and Wyllie, H. J., Nature, 218, 1135–1140 (1968).

    Article  ADS  CAS  Google Scholar 

  23. Eckenfels, A., and Vane, J. R., Br. J. Pharmac., 45, 451–462 (1972).

    Article  CAS  Google Scholar 

  24. Willis, A. K., Prostaglandins, 5, 1–25 (1974).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Wellcome Research Laboratories, Beckenham, Kent, BR3 3BS, UK

    P. Needleman, S. Moncada, S. Bunting & J. R. Vane

  2. Department of Chemistry, Karolinska Institutet, S-104 01, Stockholm, 60, Sweden

    M. Hamberg & B. Samuelsson

Authors
  1. P. Needleman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Moncada
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Bunting
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. R. Vane
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Hamberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. B. Samuelsson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Needleman, P., Moncada, S., Bunting, S. et al. Identification of an enzyme in platelet microsomes which generates thromboxane A2 from prostaglandin endoperoxides. Nature 261, 558–560 (1976). https://doi.org/10.1038/261558a0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1038/261558a0

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing